8791 results about "Inoculation" patented technology

Porcine circovirus-2 (PCV-2) is a recently identified agent wherein the potential spectrum of PCV-2-associated disease has been expanded by evidence of vertical and sexual transmission and associated reproductive failure in swine populations. PCV-2 was isolated from a litter of aborted piglets from a farm experiencing late term abortions and stillbirths. Severe, diffuse myocarditis was present in one piglet associated with extensive immunohistochemical staining for PCV-2 antigen. Variable amounts of PCV-2 antigen were also present in liver, lung and kidney of multiple fetuses. Inoculation of female pigs with a composition including an immunogen from PCV-2 or an epitope of interest from such an immunogen or with a vector expressing such an immunogen or epitope of interest prior to breeding, such as within the first five weeks of life, or prior to the perinatal period, or repeatedly over a lifetime, or during pregnancy, such as between the 6th and 8th and/or the 10th and 13th weeks of gestation, can prevent myocarditis, abortion and intrauterine infection associated with porcine circovirus-2. In addition, innoculation of male and/or female pigs with the aforementioned compositions can be carried out to prevent transmission of PCV-2 from male to female (or vice versa) during mating. Thus, the invention involves methods and compositions for preventing myocarditis, abortion and intrauterine infection associated with porcine circovirus-2.

An automatic repeater vaccinator apparatus (10) for dispensing a predetermined volume of a fluid into an animal, particularly a fluid which is a vaccine, and reloading after each volume of fluid is dispensed. The apparatus (10) comprises a handheld syringe (100) for dispensing the fluid, a flexible conduit (12) for transferring the fluid from a dispensing means to the syringe (100), and a reservoir (48) for providing the fluid to the dispensing means. The dispensing means is electrically activated by a magnetically closeable switch (preferably a reed switch (142)) in the syringe (100), which enables a predetermined volume of the fluid to be dispensed from the syringe (100) when the dispensing means is activated and reloading fluid from the reservoir (48) to replace the volume of fluid which has been dispensed from the syringe (100) when the dispensing means is deactivated. Preferably, the dispensing means comprises a pump (200 or 500) operated by a linear actuator such as a solenoid (300), which is electrically activated by the magnetically closeable switch in the syringe (100). The apparatus (10) is particularly useful for inoculating poultry, particularly inoculating the poultry by the wing web method using the needle and hub assembly (400) which comprises the filament (402) to prevent leakage between inoculations.

A hypodermic syringe and a plurality of single use cartridges able to be successively loaded into said syringe for providing rapid dispensing of a medicant to numerous users without possibility of contamination. The cartridges are continuously fed through the syringe for the quick and efficient inoculation of patients. An operator inserts a needle into a patient by pushing in a trigger. Prior to the needle being inserted into the patient, a disinfectant is dispersed from the cartridge to maintain sterility. An operator then pushes in a plunger forcing the medication through the needle into the patient. The plunger is pulled back into its original location after use. The needle is pulled back with the plunger out of the patient's skin and back into the cartridge. A new cartridge on a clip is then advanced into the syringe for use in inoculating the next patient needing medication. The syringe and cartridge dispenser further maintains a sterile environment during successive vaccinations reducing the transmission of any disease from patient to patient.

The invention discloses a method for cultivating pure white needle mushrooms. The method comprises the following steps: a strain is prepared; a culture material is prepared; bagging and sterilization are carried out; inoculation is carried out; a mycelium is cultured; nutrition, humidity, temperature, light, air and other culture conditions are strictly controlled during a fruiting period; and harvesting is carried out in an optimum period. the formulation of the culture material is 0 to 32 percent of weed tree sawdust, 0 to 93 percent of cotton seed hulls or wheat straw, straw, corncob and other crop straw, 0 to 20 percent of wheat bran, 0 to 10 percent of corn meal, 0 to 10 percent of soybean meal, 0 to 1 percent of calcium carbonate, 0 to 0.2 percent of monopotassium phosphate, 0 to 0.2 percent of magnesium sulfate, 1 to 1.2 percent of sucrose, 0 to 10 percent of rice bran, 0 to 1 percent of plaster and 0 to 0.02 percent of urea. Quicklime and wettable carbendazim are added during the preparation of the culture material so as to prevent the pollution of undesired bacteria. The technical proposal aims to select proper breeds, popularize local large scale planting and improve cultivation benefit.

Porcine circovirus-2 (PCV-2) is a recently identified agent wherein the potential spectrum of PCV-2-associated disease has been expanded by evidence of vertical and sexual transmission and associated reproductive failure in swine populations. PCV-2 was isolated from a litter of aborted piglets from a farm experiencing late term abortions and stillbirths. Severe, diffuse myocarditis was present in one piglet associated with extensive immunohistochemical staining for PCV-2 antigen. Variable amounts of PCV-2 antigen were also present in liver, lung and kidney of multiple fetuses. Inoculation of female pigs with a composition including an immunogen from PCV-2 or an epitope of interest from such an immunogen or with a vector expressing such an immunogen or epitope of interest prior to breeding, such as within the first five weeks of life, or prior to the perinatal period, or repeatedly over a lifetime, or during pregnancy, such as between the 6and 8and/or the 10and 13weeks of gestation, can lower viral titer, prevent myocarditis, abortion and intrauterine infection associated with porcine circovirus-2. In addition, innoculation of male and/or female pigs with the aforementioned compositions can be carried out to prevent transmission of PCV-2 from male to female (or vice versa) during mating. Thus, the invention involves methods and compositions for preventing myocarditis, abortion and intrauterine infection associated with porcine circovirus-2.

The invention discloses a mixture of poly-pneumococcal capsular polysaccharide-protein conjugates. The mixture contains 13 pneumococcal capsular polysaccharide-protein conjugates and an immunity-enhancement adjuvant, wherein each pneumococcal capsular polysaccharide-protein conjugate is formed by combining corresponding serum-type pneumococcal capsular polysaccharide with a same protein carrier through a covalent bond; the 13 pneumococcal capsular polysaccharides are obtained by purifying and extracting 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F and 23F bacteria; the protein carrier is A chain of a diphtherin mutant CRM197 obtained from expression of genetic recombinant escherichia coli. Meanwhile, the invention discloses a preparation method of the mixture. The mixture, compared to conventional bacterial fermentation total-chain diphtherin mutant CRM197, is easy to purify, high in yield and low in cost; experiments prove that the mixture disclosed by the invention is immunogenic, and is applicable to clinical inoculation.

In a distributed network having a number of server computers and associated client devices, method of creating an anti-computer virus agent is described. As a method, the innoculation is carried out by parsing a selected computer virus into a detection module that identifies a selected one of the client devices as a target client device, an infection module that causes the virus to infect those target client devices not infected by the selected virus, and a viral code payload module that infects the targeted client device modifying the infection module to infect those computers already infected by the selected virus; and incorporating innoculation viral code in the payload module that acts to prevent further infection by the selected virus.

The invention relates to a fermentation method for production of nutritionally balanced natural fruit enzymes. The method comprises the following steps: (1) pretreatment on raw materials; (2) a first bacterium inoculation: inoculating a saccharomyces cerevisiae liquor with concentration of 106 CFU / ml into a pulp according to an inoculation proportion of 5%; (3) early stage fermentation; (4) a secondary bacterium inoculation: after the early fermentation, inoculating acetic acid bacterium liquid and lactic acid bacterium liquid in a weight ratio of 1:1 for a secondary bacterium inoculation according to an inoculation amount of 5%; (5) middle-term fermentation; (6) coarse filtration; (7) secondary fermentation; (8) ripening; and (9) fine filtration to obtain the natural fruit enzyme. The fermentation method provided by the invention through low-temperature sterilization and strict control on the fermentation temperature retains the enzyme activity to the maximum; and twice inoculation of dominant bacteria effectively inhibits breeding of other infectious microbes, so as to realize stable product quality.

The invention discloses a tea vinegar beverage and a method for preparing the same. The tea vinegar beverage is prepared from fresh tea leaves by the steps of spreading, green killing, crushing, leaching, filtration, inoculation of microzyme, fermentation of acetic bacteria, filtration and sterilization. The tea vinegar beverage has a heavy fragrance of vinegar, a bright golden yellow liquor color, proper taste of sourness and sweetness and an obvious tea smell. According to the preparation method provided by the invention, the tea vinegar beverage is directly prepared by fermenting fresh tea leaves serving as the raw material, thereby enriching the variety of tea beverage products, contributing to the development and utilization of fresh tea leaves in China and enjoying a wide application prospect.

The invention comprises a novel method for protecting subjects against malaria. The method of the invention involves inoculation with attenuated sporozoites, and in particular, but not limited to subcutaneous, intramuscular, intradermal, mucosal, submucosal, and cutaneous administration.

An inoculation training kit contains a simulated body part and at least one inoculation needle, allowing a user to practice inoculations upon the simulated body part.

The invention provides a multivalence pneumococcus capsular polysaccharide-protein conjugate composition and a preparation method thereof. The conjugate composition is prepared from capsular polysaccharides of pneumococcus of 24 different serotypes and a carrier protein in a covalence connection manner, wherein the 24 different serotypes are 1, 2, 3, 4, 5, 6A, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F and 33F. The conjugate composition is good in adsorption effect and stability, has multiple immunogenicity and protection properties aiming at invasion of pneumococcus of the 24 serotypes, and is superior to the low-valence pneumonia composition in the market, and the immune response is higher than that of uncombined compositions. By inoculating a multivalence pneumococcus capsular polysaccharide conjugate vaccine prepared from the conjugate composition, the inoculation injection times can be reduced, the immunization procedure can be simplified, and meanwhile human beings and animals can be effectively prevented from diseases resulted from the pneumococcus of the 24 serotypes, and the conjugate composition is wide in coverage range and good in immune effect.

The invention provides a micro-organism restoration product and a restoration method used for soils polluted by petroleum and petroleum products. The micro-organism restoration product consists of degrading bacteria of hydrocarbon, biological nutrient, biology surfactant and soil activator; the four components are singly stored; in the micro-organism restoration method, the four matters are added according to a certain proportion and steps and mixed at the same time so as to complete the inoculation of biology restoration; subsequently, diluent of the biological nutrient and the soil surfactant are sprayed periodically, meanwhile, water is supplemented and the plowing and oxygen solarisation of the soil are carried out till that the required indexes are achieved. The micro-organism restoration method and the micro-organism restoration product of the invention solidify the degrading bacteria on wheat bran, solve the problem of long-term storage of the miro-organism, and can obtain the required micro-organism with the activation can be obtained by activating the micro-organism by warm water when in use; the method can adopt in-situ biological restoration or ex-situ biological restoration.

The invention discloses a method for propagating Phytoseiulus Persimilis, which comprises the steps: planting and cultivation of host plant, inoculation and feeding of Tetranychus cinnabarinus, inoculation and feeding of Phytoseiulus Persimilis as well as collection, package and refrigeration of Phytoseiulus Persimilis. The method takes mass propagation of Phytoseiulus Persimilis as the purpose, provides technical support for feeding Phytoseiulus Persimilis on scale, and provides special predacity natural enemy for Tetranychus pest mites on greenhouse crops for biological control. The invention is a production method in which living hosts (firstly host plant and host Tetranychidae are cultivated) are adopted for feeding Phytoseiulus Persimilis, and makes a series of breakthroughs on key techniques for artificial mass propagation of Phytoseiulus Persimilis. The method of the invention can lead the production cost of Phytoseiulus Persimilis to be stabilized, the production period to be 25 days, and the production scale to reach 10,000/m<2>.

The invention discloses a large-scaled culture method of high-concentration nitrosobacteria and application thereof. The method comprises the following steps of: (1) adding an enrichment culture solution with initial ammonia nitrogen mass concentration of 30-500mg/L in a reaction device with functions of stirring, heating and aeration; preparing activated sludge of a waste water treatment plant in activated sludge of 0.5-20% for primary inoculation to conduct the enrichment culture of the nitrosobacteria by 2-6d as a culture cycle, wherein extra ammonium salts and a growth promoter are added during the enrichment culture of the nitrosobacteria; (2) ending the culture when the concentration of NO2-N in the solution is accumulated to reach 70-95% of the concentration of total nitrogen; adding a flocculating agent for flocculation and sedimentation; and allowing to stand still to remove supernate, and then adding the enrichment culture solution to start the culture of the second cycle; and (3) continuously conducting 3-8 cycles of the enrichment culture to obtain the high-concentration nitrosobacteria. By adopting the nitrosobacteria to strengthen the bi-nitrification treatment of ammonia nitrogen contained waste water, the method has the advantages of high stress resistance, low cost and high efficiency and is suitable for the complicated treatment of ammonia nitrogen contained industrial waste water.

The present invention provides an aphidius gifuensis scale breeding technique, and belongs to the branch of technology of biological control of agricultural destructive insects for solving problems that the prior breeding technique of aphidius gifuensis is inefficiency and adj. industrialization is not liable to realize.The invention provides a aphidius gifuensis scale breeding technique and the embodiment scheme of the present invention comprises the following steps:(a) tobacco plant replanting: when growing to a resettling growth stage, the tobacco plants are replanted to a honeybee breeding booth;(b) myzus inoculation to tobacco plant: myzus is inoculated to the tobacco plant when the tobacco plant grows out 9-12 real leaves, and each tobacco plant is inoculated with 20-30 myzuses;(c) honeybee inoculation in parasitism: when myzuses are 2000-3000 per tobacco plant, artificial inoculation is performed, after 10-14 days, deadlocked myzuses are formed, and then after a week, the deadlocked myzuses will become aphidius gifuensis.By using the scale breeding technique of the present invention, each plant can obtain more than 8000 eadlocked myzuses, thus, the breeding cycle is shortened, and large-scale production can be performed.

The invention relates to a compound microbial agent which comprises compound bacteria for decomposing kitchen wastes and auxiliary materials for solid fermentation, wherein the compound bacteria comprise 10-15 parts of cellulose degradation bacteria, 15-30 parts of starch degradation bacteria, 15-20 parts of protein degradation bacteria, 15-20 parts of grease degradation bacteria, 10-15 parts of bacillus subtilis and 5-15 parts of pseudomonas aeruginosa; the auxiliary materials comprise 50-60 kg of wheat bran, 15-20 kg of bean pulp, 0.4-0.6 kg of magnesium sulfate, 0.4-0.6 kg of potassium dihydrogen phosphate, 0.1-0.2 kg of disodium hydrogen phosphate and 8-10 kg of glucose. The inoculation amount of the compound bacteria in the auxiliary materials is 0.8%-1%, and the compound bacteria is subjected to solid fermentation to obtain the compound microbial agent. The compound microbial agent has a good decomposing effect on greases, proteins and fiber substances and can be tolerant to salt with a certain concentration; optimally, the degradation rate can reach more than 90%.

The invention discloses vertical equipment for treating organic waste by dry anaerobic digestion without stirring and a method thereof. The equipment comprises that a feed port and an air outlet are arranged on the vertical digester; a discharge port is arranged on the bottom of the vertical digester; a feeding storage tank is connected with the discharge port of the vertical digester through a pipeline, an inoculation mixing tank and a delivery pump; the discharge port of the vertical digester is connected with the inoculation mixing tank through a control valve; a biogas slurry discharge pipe is arranged on the lower part of the vertical digester, and is connected with a spraying device arranged on the upper part of the vertical digester through a pH value on-line monitor, a reflux pump sequentially; and an alkali solution storage tank is connected with the reflux pump through the pipeline and the control valve. The vertical equipment for treating organic waste by dry anaerobic digestion without stirring and the method thereof overcome the defects of difficult isotrope mixing, easy isotrope acidification and the like of the prior dry anaerobic digestion system, and is applicable to treatment on household organic garbage, kitchen garbage, excrement of fowls and livestocks, agricultural waste, garden garbage, sewage sludge and other organic waste.

The invention discloses a composite plant enzyme containing probiotics and an application of the composite plant enzyme. The composite plant enzyme comprises amylase, lipase, protease, cellulase, beta-dextranase, xylanase and lactase. The composite plant enzyme is obtained by discontinuously feeding materials and fermenting for a plurality of periods, selecting a plurality of types of fruits and vegetables, and edible and medical plants as fermentation substrates and taking a plurality of types of probiotics/yeasts as fermentation inoculation substances through a plurality of grades of deep fermentation. The composite plant enzyme contains seven enzymes needed by a human body at the same time so that the plant enzyme has the synergistic effects of promoting in-vivo metabolism and decomposing toxic substances; the plant enzyme contains a high-activity compound enzyme and has a wide acting condition, good adaptability, and good stability, acid-base resistance and high-temperature resistance; the plant enzyme can effectively and rapidly improve the biochemical reaction speed and can be used as a raw material to be applied to foods and chemicals for daily use.

The invention discloses a special amino acid fertilizer-water paste for aquiculture and a preparation method thereof. The special amino acid fertilizer-water paste for aquiculture is prepared from the following raw materials in certain proportion: an amino acid solution, microelements, fermentation organic substances, inorganic fertilizers, minerals, a water quality improver, beneficial bacteria and a thickening agent. The preparation method comprises the following steps: A. production of amino acid solution namely, a. hydrolyzing; b. neutralizing; c. filtering; d. concentrating; B. production of fermentation organic substances: a. mixing: evenly mixing organic raw materials with the amino acid solution; b. inoculation: evenly mixing the organic raw materials with beneficial bacteria; c. fermentation; and C. production of amino acid fertilizer-water paste: a. chelation: heating the amino acid solution, adding microelements while stirring to perform chelation; b. mixing: sequentially adding the fermentation organic substances, inorganic fertilizer, minerals, water quality improver and beneficial bacteria, and evenly mixing; c. thickening: adding a thickening agent, and evenly mixing; d. inspection. The invention enhances the immunity of fish, reduces the occurrence of diseases, increases the culture yield of bighead, lowers the fish culture cost, and improves the fish quality.

The invention relates to a method for producing L-lactic acid. In the method, crop straw and Rhizopus oryzae serve as raw materials. The method particularly comprises the following operation steps: A. preparing straw saccharified liquid; B. adding auxiliary material to inoculate Rhizopus oryzae for fermentation; C repeatedly fermenting 25 batches; D. acidizing fermented clear liquid; E. ultrafiltration; F. nanofilteration; G. reverse osmosis; H. ion exchange; I. concentrating in vacuum to obtain the L-lactic acid product with the mass percentage concentration of 85%. The invention takes the straw hydrolysis saccharified liquid as the main raw material to prepare L-lactic acid, which obviously lowers the production cost of L-lactic acid; Rhizopus oryzae is adopted for once inoculation proliferation, thallus can be reused for several times to produce L-lactic acid, thus shortening L-lactic acid fermentation period, and obviously improving the fermentation strength of L-lactic acid; an advanced membrane separation and ion exchange integration technology is adopted to improve the lactic acid product quality; and the invention has simple process and strong continuity.

The present invention relates to the field of producing starter cultures. In particular, a method for customers in need of a starter culture with a consistent quality, is provided. Specifically, the method involves the use of subsets of a stock inoculum material, which comprises a concentrate of starter culture organism cells to be propagated for direct inoculation of a cultivation medium, to obtain a starter culture whereby the conventional stepwise preparation of inoculum material for the production of a starter culture can be avoided. This novel method can be used for the manufacturing of starter cultures for the food, feed or pharmaceutical industry. Furthermore, the method is useful in the cultivation of cells expressing desired products, such as primary and secondary metabolites, including e.g. enzymes and flavors.