719 results about "Inoculation" patented technology

The invention belongs to the technical field of health-care products, and particularly relates to a fermented composition beneficial to enhancing immunity and a preparation method thereof. The fermentation composition for improving immunity is prepared by taking yeast beta-glucan, an elderberry extract, glucoraphanin, sea cucumber peptide, inulin and a traditional Chinese medicine zymolyte as fermentation raw materials, firstly inoculating bacillus coagulans and staphylococcus xylosus for aerobic fermentation, and then inoculating bifidobacterium longum for anaerobic fermentation, the aerobic fermentation stage and the anaerobic fermentation stage have a synergistic effect, so that the fermentation speed can be increased, the fermentation time can be shortened, and richer nutrient substances can be provided, and therefore, the composition has an excellent effect of enhancing the immunity; the fermentation composition is applied to preparation of capsules helpful for enhancing immunity, is helpful for enhancing human immunity, and can solve the problem that active substances in existing health-care products are difficult to effectively absorb by human bodies, so that the effect of enhancing immunity is influenced.

The invention discloses an alternate medicine discharging method, belongs to the technical field of automatic distribution of vaccines, and solves the problem that in the prior art, the vaccination station is low in vaccination and emergence efficiency. The alternate medicine discharging method comprises the specific steps that the AGV medicine conveying trolley conveys vaccines into the drawer frame of the inoculation table, the vaccine guiding device is switched between the two electric drawers, the AGV medicine conveying trolley drives away from the drawer frame and conveys the vaccines into the electric drawers, and the two electric drawers are opened alternately. By arranging the AGV medicine delivery trolley, the vaccine pouring device, the drawer switching device and the vaccine guiding device, vaccines can be alternately put into the two electric drawers, and the electric drawers are vertically arranged, so that the vaccination efficiency can be greatly improved, and meanwhile, the leg space of medical staff cannot be affected.

The invention discloses an alternate medicine discharging groove, belongs to the technical field of automatic distribution of vaccines, and solves the problem of low seedling emergence efficiency of an alternate medicine discharging groove in the prior art. The alternate medicine discharging groove comprises an inoculation table, an AGV driving channel, a drawer frame, an electric medicine discharging device, an AGV medicine conveying trolley, a vaccine pouring device, a drawer switching device and a vaccine guiding device. Three vertically-arranged electric medicine discharging devices are arranged in the drawer frame, a vaccine pouring device is arranged on the AGV medicine conveying trolley, the AGV medicine conveying trolley can push the drawer switching device to enable the vaccine guiding device to change the vaccine guiding position, and vaccines in the vaccine pouring device alternately enter the two electric medicine discharging devices on the lower portion. According to the alternate medicine discharging groove, vaccines can be alternately placed into the electric medicine discharging devices on the middle portion and the lower portion, the electric medicine discharging devices are vertically arranged, two drawers for alternately receiving the vaccines can be vertically arranged on one inoculation station, the comfort degree of medical staff is not affected, and the seedling emergence efficiency is improved.

The invention provides a method for producing fragrance through co-culture of gliocladium neurospora and lachnum brachytrichum, and relates to the technical field of microorganisms. The method for producing fragrance by co-culture of the gliocladium neurosporum and the lachnum brachytrichum comprises the following steps: S1, strain activation: respectively inoculating the gliocladium neurosporum and the lachnum brachytrichum into a PSA solid culture medium for activation culture; s2, co-culture inoculation and culture: inoculating the activated gliocladium neurospora and lachnum brachytrichum in the step S1 into a PSA solid culture medium in the same culture container by adopting a five-point confrontation method; and S3, volatile matter collection and analysis: from the second day after inoculation, collecting headspace volatile matters of the culture every 48 hours by adopting a headspace solid-phase microextraction method, and then identifying the co-culture specific volatile organic compounds. Five kinds of specific VOCs which are not cultured independently can be induced to be generated, and the fragrance diversity is enriched; the method is stable and repeatable, the product contains clear aroma components, and an efficient and green new strategy is provided for natural perfume development.

The invention discloses a high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation. According to the high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation, yield increase and quality improvement are achieved through fungus material composite strengthening treatment and a composite strain synergistic inoculation technology. The method comprises the following steps: (1) fermenting a tilia brachypoda substrate by lactobacillus plantarum and soaking in a nano-composite nutrient solution to form a high-activity bacterial material; (2) nitrogen-fixing bacteria, disease-resistant actinomycetes and armillaria mellea are mixed and inoculated according to the proportion of 3: 1: 1, nitrogen supply is improved, and the occurrence rate of black rot is reduced; 3) activating a GASA stress-resistant gene of the seed hemp through a pulsed electric field (5 kV / cm, 10 microseconds), and enabling the germination rate to reach 88.5% in combination with a calcium signal channel strengthening technology; according to the method, the yield per mu of gastrodia elata is increased by 30%-40%, the gastrodin content is increased by 30% or above, the chemical pesticide dosage is reduced by 55%, the strain passage purity is stable, the method is suitable for large-scale planting in multiple climate areas, and an innovative solution is provided for green production of traditional Chinese medicinal materials.

The invention relates to a streptomyces sp. Strain EC15 and an application of the streptomyces sp. Strain EC15. The streptomyces strain EC15 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33195. The streptomyces strain EC15 has the advantages that the streptomyces strain EC15 can be used for preparing the streptomyces strain EC15; the strain has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing a biological membrane, producing an iron carrier, producing IAA and producing ACC deaminase. The EC15 bacterial liquid is used for soaking seeds under the stress of 200 mmol / L and 250 mmol / L NaCl, so that the germination potential, the germination rate, the germination index, the radicle length, the germ length and the fresh weight of group seedlings of the alfalfa seeds can be obviously improved. After the EC15 strain is inoculated, under the stress of 200 mmol / L NaCl, the plant height, stem diameter, leaf area and overground part fresh and dry weight of alfalfa plants can be obviously improved.

The utility model discloses a poultry vaccination marking mechanism which comprises an ink jet mechanism, the ink jet mechanism is flat and hollow and is connected with an external liquid pump through a hose, one side of the ink jet mechanism is provided with a nozzle, the other side of the ink jet mechanism is rotatably provided with a limiting ball, one end of the limiting ball is connected with a connecting mechanism through a connecting rod, and the other end of the limiting ball is connected with the connecting mechanism. The input end of the liquid pump is connected with an ink box containing marking ink through a pipeline, and the output end of the liquid pump is connected with the ink jetting mechanism through a hose. The trigger switch of the liquid pump can be linked with the vaccine injector, when the vaccine injector is started to inject vaccines once, the liquid pump operates once, a certain amount of ink is sprayed to feathers on poultry bodies, and the ink-jet mechanism is linked with the vaccine injection mechanism to inject the vaccines into the feathers. Meanwhile, the poultry feather marking device is small and exquisite in design, light, simple in structure, low in use and maintenance cost and suitable for small-scale poultry breeding.

The invention discloses a method for preparing organic selenium from saussurea involucrata and application of the method, and belongs to the technical field of organic selenium preparation. The preparation method of the organic selenium provided by the invention comprises the following steps: the inoculation amount of saussurea involucrata is 2.5%, a sterile Na2SeO3 solution is added after culture is performed for 2 hours, the working concentration of Na2SeO3 is 25 mu g / mL, and culture is continued for 36 hours after uniform mixing; the organic selenium is successfully prepared by adopting the preparation method provided by the invention, the final total selenium content is 1356.60 + / -23.75 mu g / g, the selenium conversion rate is 19.94 + / -0.14%, the organic selenium content is 1177.74 + / -28.82 mu g / g, and the proportion is 86.81 + / -0.89%; the provided preparation method of the selenium-rich saussurea involucrata bacteria is simple and easy to operate, has low requirements on instruments and equipment and high conversion rate of organic selenium, and can be used for industrial production of organic selenium.

The invention discloses double-milk-source fermented milk of double-peak camel milk and brown milk and a preparation method of the double-milk-source fermented milk, and the preparation method comprises the following steps: mixing raw materials: mixing the double-peak camel milk and the brown milk according to a mass ratio to obtain double-milk-source mixed milk; pretreatment: sequentially carrying out homogenization treatment and heat treatment on the double-milk-source mixed milk; strain inoculation: inoculating the pretreated double-milk-source mixed milk with a fermentation strain, wherein the fermentation strain comprises a core strain and an auxiliary strain; the core strains are lactobacillus and streptococcus thermophilus which have tolerance to antibacterial components of the camel milk, and the core strains contain strains capable of producing exopolysaccharides. Through scientific matching of lactobacillus, streptococcus thermophilus and specific auxiliary strains which have tolerance to antibacterial components of the camel milk, the inhibition effect of natural antibacterial components such as lysozyme and lactoferrin in the camel milk on conventional strains is successfully overcome.

The application discloses a kind of exogenous bacteriophage inoculation methods for strengthening soil microbial arsenic methylation capacity, comprising the following steps: S1. preparation bacteriophage crude extract;S2. preparation concentrated solution;S3. preparation bacteriophage extract;S4. preparation inoculation soil sample;S5. in the 2, 4, 6th day of culture, bacteriophage extract is added to inoculation soil;S6. after three times inoculation, continue to flood culture 9 days.The application proposes a kind of inoculation method for retaining virulent bacteriophage, not retaining original host bacteria, inoculating after flooding two days, and inoculating three times, so that the proportion of lysogenization of bacteriophage in soil after inoculation is increased by 156%~347%, so that arsenic metabolism genes carried by bacteriophage genome can function, the arsenic methylation capacity of soil microorganism is strengthened, and the arsenic methylation capacity is increased by more than 300%, to efficiently methylate arsenic in soil.

The invention relates to the technical field of food processing machinery, discloses an inoculation device for natto production and a production method, and aims to solve the problems of non-uniform inoculation, bacterial liquid waste, incapability of continuous production and the like in the prior art. The device comprises a tank body assembly, a material turning and path switching mechanism, a jacking type gate mechanism, an electrostatic spraying system and an online quality monitoring system. The tank body is divided into a mixing cavity and a backflow cavity by an arc-shaped partition plate. The core is that the material turning and guiding piece rotating in one direction is provided with a first guiding face and a second guiding face which have different functions, and the material turning and guiding piece and the liftable lifting table work cooperatively. In the working process, the lifting table is at a high position, materials are lifted up by the first flow guide face and roll down, circular flow is formed through the backflow cavity, and in the process, the electrostatic spraying system accurately adsorbs liquid with bacteria to beans; the on-line monitoring system judges the uniformity in real time, the lifting platform descends to a low position after the uniformity reaches the standard, and the material turning and guiding piece guides the material to the material discharging and guiding plate to complete automatic material discharging.

The invention discloses application of a tea tree CsGT-3b gene in improving the disease resistance of plants and a method of the tea tree CsGT-3b gene. The CsGT-3b gene is a Trihelx transcription factor family gene, the CsGT-3b gene is constructed to a plant expression vector and is transferred into tobacco, a transgenic tobacco plant is obtained through stable genetic transformation, and the CsGT-3b gene shows relatively strong fungal inhibitory activity on botrytis cinerea inoculation; according to the present invention, the CsGT-3b gene on the tea tree leaf is subjected to instantaneous silencing by using the antisense oligonucleotides (AsODNs) technology, such that the disease resistance of the plant on the lasiodiplodia theobroma is significantly weakened, and the CsGT-3b gene on the tea tree leaf is subjected to the enzyme-linked immunosorbent assay so as to significantly reduce the disease resistance of the plant on the lasiodiplodia theobroma; therefore, the CsGT-3b gene has the effect of improving the disease resistance of the plants, can be used as a disease-resistant gene, can improve the disease resistance of the plants by being introduced into the plants such as tobacco, tea trees or vegetables, and has a wide market application prospect.

The invention discloses 10-hydroxydecanoic acid as well as a preparation method and application thereof, and belongs to the technical field of cosmetics, Candida lipolytica is taken as a fermentation thallus, the Candida lipolytica contains alkane monooxygenase (CYP450), alcohol dehydrogenase (ADH), fatty alcohol oxidase (FAO) and fatty aldehyde dehydrogenase (FALDH), amplification and activation are performed on the Candida lipolytica in a seed culture medium, and the Candida lipolytica is obtained. The method comprises the following steps: inoculating a seed solution into a liquid culture medium, enabling a strain to adapt to a culture environment in advance, reducing strain death caused by environmental mutation after inoculation, inoculating the seed solution into the liquid culture medium, culturing until OD600 is 45-55, realizing high-density enrichment of the strain, being beneficial to increasing the content of CYP450, ADH, FAO and FALDH, adding decane and Tween 80, and enabling the Tween 80 to be capable of increasing the permeability of a cell membrane and increasing the reaction rate, the CYP450 is used for catalyzing decane to carry out hydroxylation, and ADH, FAO and FALDH are used for carrying out oxidation, so that the high-purity 10-hydroxydecanoic acid is obtained.

The invention discloses a novel soybean downy mildew resistance molecule detection method. The invention provides a method for predicting soybean downy mildew resistance, which comprises the following steps: 1) acquiring the expression quantity ratio of Pm18s gene to GmActin gene in inoculated leaves of to-be-detected soybean inoculated with downy mildew, and recording the expression quantity ratio as Pm18s / GmActin ratio as sample data; and 2) predicting the downy mildew resistance of the soybean to be detected according to the sample data. The method comprises the following steps of: inoculating downy mildew bacteria on the back of a main leaf by using a smearing method by taking a soybean of which the first main leaf is completely expanded as a material, extracting RNA (Ribonucleic Acid) 8 days after inoculation, measuring the ratio of a downy mildew bacteria Pm18s gene / soybean Actin by semi-quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction), and screening resistant soybeans. The technology not only has the advantages of accurate detection result and no climate influence, but also can greatly shorten the identification period and save land and labor.

The invention discloses a method for cultivating a heat-resistant broad-spectrum durable rice blast-resistant rice breeding material based on polygene polymerization. According to the method, a high-heat-resistance and high-dominance indica-japonica hybrid indica rice restorer line is selected as a recurrent parent, a breeding intermediate material for polymerizing complementary broad-spectrum rice blast resistance genes Pigm and Pi-jx is used as a donor parent, and polymerization of the heat-resistance and broad-spectrum lasting rice blast resistance genes Pigm and Pi-jx is realized through a method of combining molecular breeding with conventional breeding. Meanwhile, by selecting representative strains to carry out artificial inoculation identification of seedling blast and panicle blast, natural induction identification of a rice blast retransmission area and heat resistance identification, and by combining main agronomic trait evaluation in the whole growth period, a heat-resistant broad-spectrum durable rice blast-resistant breeding material is obtained. The breeding material bred by using the method disclosed by the invention has relatively strong heat resistance and broad-spectrum and lasting rice blast resistance, and the breeding process of heat-resistant and rice blast-resistant rice varieties is accelerated.

The present application relates to the technical fields of corn disease resistance research, a kind of corn stem stem after drilling wound smearing tool, including shell, the shell inside is provided with connecting shaft, one end of the connecting shaft is fixedly connected with smearing part, another shell is also provided with the drive mechanism of moving back and forth with the connecting shaft and smearing part, another in the shell is provided with medicine box, the medicine box outside is connected with lifting connecting mechanism, the lifting connecting mechanism is connected with drive mechanism, for driving the medicine box moves up and down.The present application is moved by the cooperation of drive mechanism and connecting shaft, drives smearing part to rotate while moving, the ointment on smearing part is applied to the inner wall of small hole, and the small hole on the corn stem is protected, which provides guarantee for subsequent smooth observation of corn inoculation colony manifestation, in addition, the present application is moved up and down by controlling medicine box, so that smearing part will automatically dip the ointment in medicine box during movement, facilitate continuous work of smearing part, improve efficiency.

This invention discloses a processing technology for antibody-enhancing probiotic feed, which includes raw material pretreatment, substrate preparation, sterilization, cooling, strain activation, aseptic inoculation, constant-temperature anaerobic fermentation, immune-enhancing compounding, filtration and homogenization, and aseptic filling and storage. The fermentation substrate liquid includes purified water, molasses, yeast extract, compound trace elements, and buffer salts. A compound microbial community composed of *Lactobacillus plantarum*, *Bacillus subtilis*, *Bifidobacterium*, and yeast is used for fermentation. The temperature is controlled at 35–39°C during fermentation, and intermittent stirring is employed. After fermentation, astragalus polysaccharide, echinacea extract, and trehalose are added for compounding. This invention reduces the loss of active ingredients by adding immune-enhancing components after fermentation, and improves the survival rate of probiotics and fermentation stability through intermittent stirring. The resulting liquid feed has a high live bacteria content and immune-enhancing effect.

This invention discloses a *Armillaria mellea* contact induction device and control system for improving the inoculation rate after *Gastrodia elata* seed germination. It relates to the field of co-cultivation technology of edible and medicinal fungi and medicinal plants, and includes a shell, a germination support module, a mycelial induction module, a contact bridge module, a nutrient pulse module, an environmental modulation module, a dual-evidence detection module, and a main control module. The main control module generates an inoculation window index and, after trial contact, switches between a maintained contact state and a withdrawal state based on the recovery curve to complete the contact induction and colonization maintenance of *Armillaria mellea* with *Gastrodia elata* protocorms.

This invention belongs to the field of culture medium preparation technology, specifically a device for preparing a culture medium for Poria cocos spawn. The device includes a frame with a hopper mounted on it. One side of the hopper has a discharge pipe and an auxiliary bagging sleeve for bagging. The other side of the hopper has an inoculation channel generating component. The inoculation channel generating component includes a first auger rotatably mounted inside the discharge pipe. The first auger has a hollow central shaft, with one end of the central shaft extending out of the hopper and communicating with a feed pipe. The feed pipe is clearance-fitted with the central shaft. An extrusion tube is positioned above the feed pipe. This invention significantly improves the efficiency of culture medium bagging and inoculation. It also provides a slow-release effect for nutrients, continuously supplying nutrition for mycelial growth. Furthermore, because the extrusion component remains inside the bag during bagging, there is no need for careful handling to prevent channel collapse after the stick is pulled out, further facilitating automated inoculation.

There is a modified coronavirus spike antigen protein and uses therefor. The spike antigen protein of coronavirus exhibits suppression of cell membrane fusion ability and improves safety by modifying two protein cleavage sites present in the coronavirus spike protein. In addition, inoculation with a vaccine having the antigen protein induces production of a large number of neutralizing antibodies to inhibit invasion of coronavirus into cells, thereby suppressing viral proliferation.

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.

The invention provides application of platanus sp. Su100 in promotion of synthesis of poplar melatonin and disease resistance, and relates to the technical field of microbial resource development and forest cultivation. The soft disc fungus Su100 can activate the activity of a key enzyme for synthesizing melatonin in a poplar body, and the content of the melatonin is remarkably increased. As a plant endogenous antioxidant and a multifunctional signal molecule, the melatonin plays a core role in plant stress resistance, growth regulation and control, quality improvement and ecological restoration. The floppy disc fungi have high colonization efficiency in plants, and can form a symbiotic system with the poplar by utilizing a floppy disc fungi inoculation technology to play a role for a long time. The application of the invention can significantly reduce the production cost of agriculture and forestry, is especially suitable for crop cultivation under the adverse conditions of drought, salt and alkali, diseases and the like, reduces the application of chemical fertilizers and pesticides, and lays a foundation for the construction of an efficient, green and sustainable forestry planting and cultivation system.

The application provides a preparation method of polysaccharide vaccine diluent and application to chicken newcastle disease vaccine, and belongs to the technical field of biopharmacy.The application provides a south five canes polysaccharide vaccine diluent and a preparation method thereof, and solves the problems of low uniformity of antibody titer, stress reaction and allergic reaction of the body after inoculation, and influence on the immunization effect of the vaccine caused by the physiological saline or the phosphate buffer solution diluent for diluting the vaccine.The south five canes polysaccharide vaccine diluent can be applied to animal vaccines, including chicken newcastle disease vaccine, and solves the problem that there is no polysaccharide diluent for the chicken newcastle disease vaccine in the prior art.

AAV vectors are ideally suited for long-term delivery of a combination of broadly neutralizing antibodies to achieve sterilizing immunity to HIV. Unfortunately, host immune responses to the delivered antibody have severely limited the efficacy. The TLR9 pathway has been identified in initiating adaptive immune responses against AAV and delivered bNAbs. To enhance this strategy, we validated Hydroxychloroquine, a known drug inhibitor of the TLR9 pathway, as a pretreatment to AAV delivery to avoid anti-drug antibody responses. TLR9 signaling inhibition was validated using a HEK-Blue hTLR9 reporter cell line and CpG stimulation. Hydroxychloroquine was also validated in a 3-macaque trial where AAV9-3BNC117 and AAV9-10-1074 were administered along with 3 doses of hydroxychloroquine once a week starting 1 week before AAV inoculation. Unlike historical controls, where 3BNC117 and 10-1074 expression is lost within the first 4-5 weeks, 2 macaques maintained 10-1074 expression and 1 macaque maintained 3BNC117 for the duration of the trial. Anti-3BNC117 antibodies were only observed in 2 of the 3 macaques and were significantly delayed. Anti-10-1074 antibody responses were a log lower than typically observed in historic controls. The use of hydroxychloroquine as a pretreatment for AAV inoculation is a promising strategy. The significant decrease in anti-10-1074 antibody levels and the successful delivery of 10-1074 in 2 macaques and 3BNC117 in 1 macaque was very encouraging. Extending the dosage of hydroxychloroquine beyond 3 doses may be sufficient to observe long-term bNAb expression in all animals and further decrease ADA responses. Together, these data suggest that the short-term treatment of hydroxychloroquine at the time of AAV inoculation has a meaningful impact on tolerance induction to our AAV-delivered bNAbs.

This invention discloses a cultivated rice from Africa pi- cnv9 The application of genes in improving rice disease resistance belongs to the field of genetic engineering technology. This invention has screened a gene associated with rice blast resistance. pi-cnv9 The gene, whose nucleotide sequence is shown in SEQ ID NO.1, was used in this invention to construct complementary transgenes for *Rhizophora flavescens* and *Rhizophora esculenta*, respectively. pi-cnv9 Rice plants with complementary genes. Inoculation experiments were conducted on wild-type and transgenic plants, and the results demonstrated that complementary transgenic... pi-cnv9 Genetically modified plants are more resistant to disease than wild-type plants; indicating that... pi-cnv9 Genes that positively regulate the disease resistance of rice can be used as target genes for genetic improvement and molecular breeding of rice resistance to rice blast, which is of great significance for breeding rice varieties with improved disease resistance.

The invention discloses application of a glutathione transferase gene EuGST in regulating and controlling ear rot and stem rot resistance of maize fusarium graminearum, and belongs to the technical field of gene engineering. The invention discloses an application of a glutathione transferase gene EuGST in regulation and control of corn fusarium graminearum ear rot and stem rot resistance. A nucleotide sequence of the glutathione transferase gene EuGST is shown as SEQ ID NO.1. The invention also discloses an application of the glutathione transferase gene EuGST in regulation and control of corn fusarium graminearum ear rot and stem rot resistance. Fusarium graminearum is inoculated to ears and stems of an overexpressed EuGST transgenic line, so that the ear rot and stem rot resistance of the fusarium graminearum of the maize inbred line is improved; fusarium graminearum is inoculated to ears and stems of the overexpressed EuGST transgenic hybrid, so that the ear rot and stem rot resistance of the fusarium graminearum of the corn hybrid is improved.

The application belongs to the technical field of biological nanomaterials, and particularly relates to an immune activation nanosheet as well as a preparation method and application thereof. The immune activation nanosheet comprises a two-dimensional montmorillonite nanosheet with a sheet layer structure, and manganese ions and tumor antigens loaded in the interlayer of the two-dimensional montmorillonite nanosheet. The mass ratio of the manganese ions to the montmorillonite is 1:5-1:20, and the mass ratio of the tumor antigens to the montmorillonite is 1:1-1:10. The immune activation nanosheet has the characteristics of good oral stability, high mucosal targeting, strong immune efficacy, etc. The stability of the immune activation nanosheet in the gastrointestinal tract is good, the immune activation nanosheet can be effectively enriched in the small intestine, a vaccine inoculation pool is formed, the intestinal immune tolerance can be overcome, the immune effect is enhanced, and the immune activation nanosheet has a clinical application prospect.

The invention relates to the technical field of sugarcane smut resistance, and provides a molecular marking method for sugarcane smut resistance, which comprises a sample preparation unit, a double-sugarcane area inoculation identification unit, a whole genome association analysis unit, an SNP (Single Nucleotide Polymorphism) marker development unit, a marker verification unit and a result application unit, the output end of the sample preparation unit is connected with the input end of the double-sugarcane-area inoculation identification unit through a sample transmission channel, the double-sugarcane-area inoculation identification unit outputs phenotypic data to the whole-genome association analysis unit, and the whole-genome association analysis unit outputs remarkably associated SNP sites to the SNP marker development unit; and the SNP marker development unit outputs a specific primer to the marker verification unit. The molecular marker provided by the invention is based on the correlation between the candidate gene of the site and the known disease-resistant pathway of the plant, and the MYB transcription factor can improve or reduce the resistance level of the plant to pathogenic bacteria by regulating the synthesis of secondary metabolites, transduction of plant hormone signals, expression of defensive genes and the like.

The utility model provides a kind of organic fragrant mushroom culture medium rapid inoculation equipment, including main body, feeding conveyor belt, inoculation post, feeding hopper, the side of feeding conveyor belt is provided with discharging conveyor belt, feeding conveyor belt and discharging conveyor belt between are provided with bagging mechanism, bagging mechanism includes the mounting bracket of rotation installation in the side of feeding conveyor belt, the side of mounting bracket is rotatably installed with driving gear ring, the outside of driving gear ring is engaged with installation and is equipped with multiple groups of driven gear ring.The utility model is cooperated with chuck by bagging mechanism, XY axis linear guide rail, expand the flange plate synchronous opening and closing by rack, the packaging bag is accurately adsorbed and is moved by cylinder drive chuck and is sleeved on the outer wall of flange plate, realize the automatic flanging and bagging of packaging bag, replace manual bagging operation, reduce manual intervention, significantly improve bagging efficiency;Conveying chain plate is separately arranged on the upper and lower sides of discharging conveyor belt, realize the continuous supply and recovery of packaging bag, match high-speed inoculation rhythm.

The invention discloses a thrip resistance identification device and application. The device comprises an inoculation barrel, a preservation substrate is laid at the bottom in the inoculation barrel, an isolation structure with one or more through holes covers the preservation substrate, and a sealing structure with one or more air holes covers an opening in the top of the inoculation barrel. When the device is used, the whole plant does not need to be cultivated, in-vitro flowering branches can be picked for identification, rapid screening of large-scale germplasm resources can be achieved, and repeatability and consistency of experimental results are good. Meanwhile, when the device is used, buds and functional leaves of in-vitro flowering branches are reserved, the actual feeding part of the Frankliniella occidentalis is simulated, and compared with in-vitro leaf inoculation, the real resistance level can be better reflected. The device has the advantages of easily available materials, low cost and strong operability, can be used for identifying the resistance of the Frankliniella occidentalis of various materials such as chrysanthemum and related wild species, cultivated species, breeding offspring and the like, provides a high-quality parent material for insect-resistant breeding, can be used for researching an insect-resistant mechanism, and is suitable for popularization and application in basic breeding units.