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1066 results about "Inoculation" patented technology

The terms inoculation, vaccination, and immunization are often used synonymously to refer to artificial induction of immunity against various infectious diseases. However, there are some important historical and current differences. In English medicine, inoculation referred only to the practice of variolation until the very early 1800s. When Edward Jenner introduced smallpox vaccine in 1798, this was initially called cowpox inoculation or vaccine inoculation. Soon, to avoid confusion, smallpox inoculation continued to be referred to as variolation (from Variola = smallpox) and cowpox inoculation was referred to as vaccination (from Jenner's use of variolae vaccinae = smallpox of the cow). Then, in 1891, Louis Pasteur proposed that the terms vaccine and vaccination should be extended to include the new protective procedures being developed. Immunization refers to the use of all vaccines but also extends to the use of antitoxin, which contains preformed antibody such as to diphtheria or tetanus exotoxins. Inoculation is now more or less synonymous in nontechnical usage with injection and the like, and questions along the lines of "Have you had your flu injection/vaccination/inoculation/immunization?" should not cause confusion. The focus is on what is being given and why, not the literal meaning of the technique used.

Microbacterium capable of producing siderophore, dissolving potassium, promoting growth and relieving saline-alkali stress of plants and application of microbacterium

The invention discloses a microbacterium capable of producing siderophores, dissolving potassium, promoting growth and relieving saline-alkali stress of plants and application of the microbacterium. The invention relates to the field of microorganisms, and provides a dendrobium nobile microbacterium, the dendrobium nobile microbacterium is a dendrobium nobile microbacterium, the strain number of the dendrobium nobile microbacterium is 2T1, and the registration number of the dendrobium nobile microbacterium in the China General Microbiological Culture Collection Center is CGMCC No. 32856. The strain is a new strain of microbacterium and has the capabilities of secreting siderophores and dissolving potassium. Pot experiments show that compared with uninoculated control, the root length and dry weight of wheat can be improved after the strain is inoculated under saline-alkali stress. The microbacterium dendrobii 2T1 can be used as a microbial organic fertilizer, and is used for improving the soil fertility, relieving the saline-alkali stress of crops and improving the saline-alkali adaptability of the crops.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI +1

Fermentation composition helpful for enhancing immunity and preparation method thereof

The invention belongs to the technical field of health-care products, and particularly relates to a fermented composition beneficial to enhancing immunity and a preparation method thereof. The fermentation composition for improving immunity is prepared by taking yeast beta-glucan, an elderberry extract, glucoraphanin, sea cucumber peptide, inulin and a traditional Chinese medicine zymolyte as fermentation raw materials, firstly inoculating bacillus coagulans and staphylococcus xylosus for aerobic fermentation, and then inoculating bifidobacterium longum for anaerobic fermentation, the aerobic fermentation stage and the anaerobic fermentation stage have a synergistic effect, so that the fermentation speed can be increased, the fermentation time can be shortened, and richer nutrient substances can be provided, and therefore, the composition has an excellent effect of enhancing the immunity; the fermentation composition is applied to preparation of capsules helpful for enhancing immunity, is helpful for enhancing human immunity, and can solve the problem that active substances in existing health-care products are difficult to effectively absorb by human bodies, so that the effect of enhancing immunity is influenced.
Owner:DING MA GELI (BEIJING) BIOTECHNOLOGY CO LTD

Recombinant live virus vector rabies vaccine without neurotoxicity

The invention discloses a neurotoxicity-free recombinant live virus vector rabies vaccine, which is prepared by replacing G protein of vesicular stomatitis virus with G protein of HEP-Flury rabies virus strain or G protein mutant of HEP-Flury rabies virus strain, and saving recombinant virus VSV-HEP-Flury by using a reverse genetic manipulation technology. The recombinant live virus vector rabies vaccine has no neurotoxicity after being inoculated. The method is free of neurotoxicity, high in safety, simple in inoculation procedure, good in immune effect and high in productivity.
Owner:HEFEI BAIYU BIOTECHNOLOGY CO LTD +1

Bacillus amyloliquefaciens C-50-11 with high H2O2 yield and application of bacillus amyloliquefaciens C-50-11

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus amyloliquefaciens with high H2O2 yield and application of the bacillus amyloliquefaciens. According to the bacillus amyloliquefaciens C-50-11, H2O2 can be continuously generated in the high-temperature period of compost, H2O2 free radicals can oxidize and decompose complex organic matter to promote humus condensation in a mediated mode, the content of humus in the compost in the high-temperature period is further effectively increased, and compared with the content of humus in uninoculated compost, the content of the humus after inoculation is increased by 20% or above. According to the method disclosed by the invention, chemical degradation is realized by combining an endogenous H2O2 strengthening effect of thermophilic bacteria and utilizing an oxidizing agent generated by organisms, so that the condition of a high-temperature period of compost is fully utilized, and a new method way is provided for increasing the content of humus in the compost.
Owner:CHINA AGRI UNIV

Construction and application of a reverse genetic manipulation platform for nadc34-like porcine reproductive and respiratory syndrome virus

The present application relates to a virus of reverse genetic operation construction of NADC34-like PRRSV2. Specifically, the full gene sequence of NADC34-like PRRSV2 BJ1805-2 isolated strain is segmented and connected to the vector by using PCR amplification, enzyme digestion and other methods with pACY177 plasmid as the carrier, and the BJ1805-2 full-length infectious clone recombinant plasmid (rBJ1805-2) is obtained. The infectious clone virus rBJ1805-2 is rescued in vitro, and the first NADC34-like PRRSV2 strain infectious clone platform in China is successfully built. The present application also relates to a modification method for constructing NADC34-like PRRSV2 strain which can adapt to Marc-145 cell passage culture. The infectious clone virus constructed by the present application has good in vitro and in vivo proliferation efficiency, and can cause specific viremia after inoculation in pigs; it has good safety, does not cause fever after inoculation in pigs, and will not cause piglet death. The modified strain can be used as a candidate strain for developing the first NADC34-like PRRSV2 specific vaccine in China, and is conducive to the prevention and control of PRRSV epidemic in China.
Owner:YANGZHOU UNIV

Alternate medicine discharging method

The invention discloses an alternate medicine discharging method, belongs to the technical field of automatic distribution of vaccines, and solves the problem that in the prior art, the vaccination station is low in vaccination and emergence efficiency. The alternate medicine discharging method comprises the specific steps that the AGV medicine conveying trolley conveys vaccines into the drawer frame of the inoculation table, the vaccine guiding device is switched between the two electric drawers, the AGV medicine conveying trolley drives away from the drawer frame and conveys the vaccines into the electric drawers, and the two electric drawers are opened alternately. By arranging the AGV medicine delivery trolley, the vaccine pouring device, the drawer switching device and the vaccine guiding device, vaccines can be alternately put into the two electric drawers, and the electric drawers are vertically arranged, so that the vaccination efficiency can be greatly improved, and meanwhile, the leg space of medical staff cannot be affected.
Owner:SHEN SU AUTOMATION TECH DEV CO LTD

Biological control fermentation liquor as well as preparation method and application thereof

The invention belongs to the technical field of biological control, and particularly relates to biological control fermentation liquor and a preparation method and application thereof, the preparation method comprises the following steps: S1, raw material treatment: selecting solidago canadensis as a plant raw material, cutting the solidago canadensis into pieces, performing autoclaved sterilization, adding water, and mixing to obtain a mixture; s2, adding the cultured bacillus subtilis and saccharomyces cerevisiae into the mixture in the step S1, mixing and inoculating, and stirring and fermenting to obtain a fermentation stock solution; s3, centrifuging the fermentation stock solution, taking supernate, and filtering to obtain the biological control fermentation liquor. The biological control fermentation liquor prepared by the invention can be used for controlling seed germination, seedling growth and flowering processes of solidago canadensis, and meanwhile, the biological control fermentation liquor can also be used for preparing products for controlling solidago canadensis. The problem that a biological medicament for preventing and treating solidago canadensis is lacked at present is solved.
Owner:JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY

Plant gene editing vector based on TRV, kit and gene editing method

The invention provides a structure, a sequence and a construction method of a plant virus gene editing vector and application of the plant virus gene editing vector in plant gene editing. The method is characterized in that a TRV2 genome of a tobacco embrittlement virus (TRV) is used for carrying and expressing a small Cas gene AsCas12f and gRNA of the small Cas gene AsCas12f. Preferably, the AsCas12f and the gRNA of the AsCas12f are subjected to tandem expression by utilizing the TRV2 to form an expression cassette structure of the AsCas12f-tRNA-gRNA, the expression cassette substitutes a part of 2b gene sequence of the TRV2 on a TRV2 genome to form a plant gene editing vector pTRV2-Cas12f-gRNA based on the TRV virus, and long-time stable expression and gene editing can be performed after wild type nicotiana benthamiana is inoculated. The invention also provides a method for carrying out gene editing in a wild type plant by utilizing the plant virus.
Owner:SANYA RES INST OF CHINESE ACAD OF TROPICAL AGRI +1

Dendrobium officinale unmanned inoculation tissue culture method and application thereof

The invention discloses a dendrobium officinale unmanned inoculation tissue culture method and application thereof. Belongs to the technical field of tissue culture. The vertical gradient culture medium is adopted for the first time, and automatic conversion of the development stage is achieved; a culture medium osmotic pressure gradient design guides directional growth of a root system; according to the nutrition phase change technology, the agar concentration difference of an induction layer, a differentiation layer and a strong seedling layer is utilized, the physical characteristic gradient of a culture medium is achieved, unmanned inoculation tissue culture of dendrobium officinale seeds is achieved through automatic equipment and an optimized tissue culture process, and the problems that in the prior art, artificial inoculation is low in efficiency, high in pollution rate and cost and long in culture period are solved; the efficiency, the quality and the economic benefit of dendrobium officinale tissue culture are improved, and the requirements of large-scale factory production can be met.
Owner:GUIZHOU FANTIAN AGRI TECH CO LTD

Alternate medicine discharging groove

The invention discloses an alternate medicine discharging groove, belongs to the technical field of automatic distribution of vaccines, and solves the problem of low seedling emergence efficiency of an alternate medicine discharging groove in the prior art. The alternate medicine discharging groove comprises an inoculation table, an AGV driving channel, a drawer frame, an electric medicine discharging device, an AGV medicine conveying trolley, a vaccine pouring device, a drawer switching device and a vaccine guiding device. Three vertically-arranged electric medicine discharging devices are arranged in the drawer frame, a vaccine pouring device is arranged on the AGV medicine conveying trolley, the AGV medicine conveying trolley can push the drawer switching device to enable the vaccine guiding device to change the vaccine guiding position, and vaccines in the vaccine pouring device alternately enter the two electric medicine discharging devices on the lower portion. According to the alternate medicine discharging groove, vaccines can be alternately placed into the electric medicine discharging devices on the middle portion and the lower portion, the electric medicine discharging devices are vertically arranged, two drawers for alternately receiving the vaccines can be vertically arranged on one inoculation station, the comfort degree of medical staff is not affected, and the seedling emergence efficiency is improved.
Owner:SHEN SU AUTOMATION TECH DEV CO LTD

Method for producing fragrance by co-culture of gliocladium neurosporum and lachnum brachytrichum

ActiveCN121555582AFungiComponent separationBiotechnologyGongronella butleri
The invention provides a method for producing fragrance through co-culture of gliocladium neurospora and lachnum brachytrichum, and relates to the technical field of microorganisms. The method for producing fragrance by co-culture of the gliocladium neurosporum and the lachnum brachytrichum comprises the following steps: S1, strain activation: respectively inoculating the gliocladium neurosporum and the lachnum brachytrichum into a PSA solid culture medium for activation culture; s2, co-culture inoculation and culture: inoculating the activated gliocladium neurospora and lachnum brachytrichum in the step S1 into a PSA solid culture medium in the same culture container by adopting a five-point confrontation method; and S3, volatile matter collection and analysis: from the second day after inoculation, collecting headspace volatile matters of the culture every 48 hours by adopting a headspace solid-phase microextraction method, and then identifying the co-culture specific volatile organic compounds. Five kinds of specific VOCs which are not cultured independently can be induced to be generated, and the fragrance diversity is enriched; the method is stable and repeatable, the product contains clear aroma components, and an efficient and green new strategy is provided for natural perfume development.
Owner:SOUTHWEST FORESTRY UNIVERSITY

Monitoring and early warning system for adverse reaction after vaccination

The invention relates to the technical field of medical monitoring, in particular to a monitoring and early warning system for adverse reactions after vaccination, which comprises a circadian rhythm recognition module, an extreme value offset processing module, a stage reaction mapping module, an abnormal grade recognition module and a linkage path judgment module. According to the method, a cross matching relation between abnormal response and rhythm change is established through positioning of a day and night body temperature extreme value interval midpoint before and after inoculation and continuous tracking of a time axis coordinate change trend, and whether the abnormality has rhythm driving characteristics or not is judged through a time period alignment relation between abnormality occurrence time and a rhythm offset interval. According to the method, an early warning path identification chain is constructed, participation items are expanded to sequence trend change, rhythm structure axial displacement, abnormal signal coverage rate and other elements, the sensitive capture capability for potential risks of adverse reactions after vaccination is improved, and the linkage early warning output capability for time-structure dual abnormal performance is also enhanced.
Owner:HANGZHOU CENT FOR DISEASE CONTROL & PREVENTION

Cholesterol-modified cationic liposome tumor vaccine, preparation method therefor, and use thereof

The present invention belongs to the technical field of cancer immunotherapy, and particularly relates to a cholesterol-modified cationic liposome tumor vaccine, a preparation method therefor, and use thereof. In order to solve the problems of poor targeting and strong side effects of TLR agonists in anti-tumor treatment, the present invention provides a cationic liposome prepared from a cholesterol-modified 1V209 molecule, a cationic lipid component, cholesterol, and DSPE-PEG2000, and then the cationic liposome and ovalbumin 5 form the tumor vaccine by electrostatic adsorption. Animal experiments show that the vaccine can induce antigen-specific CD8+ T cells, activate lymphocytes, and generate stronger antigen cross-presentation, more memory T cells, antibodies, and cytokines. Prophylactic inoculation with the vaccine can significantly delay the progression of mouse melanoma and lymphoma and prolong the survival of mice. The combination use of the vaccine and a PD-1 checkpoint inhibitor can further enhance the anti-tumor effect. Therefore, the vaccine is a promising cancer vaccine.
Owner:SICHUAN UNIV

Treatment method for fermenting smelly mandarin fish by coupling gradient salt control and directional inoculation

The invention discloses a treatment method for fermenting smelly mandarin fish by coupling gradient salt control and directional inoculation, which comprises the following steps of: pretreating the smelly mandarin fish, putting the smelly mandarin fish into ozone-containing water for bacteria reduction treatment, then putting the smelly mandarin fish into a salt solution for pickling, finally inoculating bacteria liquid into the pickled smelly mandarin fish for fermentation treatment, cleaning, draining, packaging and quickly freezing after fermentation is finished, and thus obtaining the smelly mandarin fish. According to the method, the fermentation production process of the smelly mandarin fish is adjusted and standardized, the microorganism content of the slaughtered smelly mandarin fish is reduced through pretreatment, then the bacteria-reduced smelly mandarin fish is pickled with a low-concentration sodium chloride aqueous solution, meanwhile, the screened strains (psychrobacter halophilus and lactobacillus plantarum) are inoculated, and compared with traditional fermentation, the method is easy to operate, low in cost and high in yield. The product quality is stable, the defective rate is low, the labor cost is saved, the speed is high, the volatile basic nitrogen of the obtained smelly mandarin fish is less than or equal to 30mg / 100g, and the peroxide value (in terms of fat) is less than or equal to 2.5 g / 100g.
Owner:JIANGNAN UNIV

Ganoderma lucidum fermentation liquor and preparation method thereof

The invention belongs to the technical field of fungal fermentation, and particularly discloses ganoderma lucidum fermentation liquor and a preparation method thereof. The preparation method of the ganoderma lucidum fermentation liquor comprises the following steps: (1) activating a strain, namely inoculating the strain into a slant culture medium for culturing, and carrying out transfer grafting once to obtain an activated strain; (2) liquid culture: inoculating the activated strain into a liquid culture medium, and carrying out shake culture for 7 days to obtain a liquid strain; (3) fermentation culture: inoculating the liquid strain into a fermentation culture medium according to the inoculation amount of 7-9%, and carrying out three-stage culture to obtain a fermented product; and (4) centrifugal filtration: centrifuging the fermented product, collecting a supernatant, and filtering the obtained supernatant to obtain the ganoderma lucidum fermentation broth. According to the preparation method disclosed by the invention, three-stage variable-condition culture is specifically carried out according to the growth condition of the ganoderma lucidum mycelia, so that rapid growth of the ganoderma lucidum mycelia and mass accumulation of polysaccharides are facilitated, and the polysaccharide content of the prepared ganoderma lucidum fermentation liquor is increased.
Owner:GUANGZHOU HANHE LIFE SCIENCES CO LTD

Macrosporium globosum capable of efficiently producing spores and promoting plant growth and application of macrosporium globosum

The invention discloses a strain of globosoma macrosporium capable of efficiently producing spores and promoting plant growth and application of the globosoma macrosporium. The preservation number of the globular cystospora margarita LBN1103 is GDMCC (China General Microbiological Culture Collection Center) No: 66650, and the preservation number of the globular cystospora margarita LBN1103 is GDMCC No: 66650. The globular cystomycosis LBN1103 has extremely high spore production capacity, and the spore production proportion is about 1: 353.4 and is 5.20-18.03 times that of globular cystomycosis strains reported in other literatures. On the premise that the inoculation amount is very low, the biomass of paspalum natatum is remarkably increased by inoculating the globular cystosporium macrosporum LBN1103 into the sterilized soil, and the biomass of tomatoes is also remarkably increased by inoculating the globular cystosporium macrosporum LBN1103 into the unsterilized soil. The globosporium macrosporum LBN1103 disclosed by the invention is high in spore production efficiency and strong in ecological niche competitive advantage, obviously promotes plant growth, and has a wide application prospect in the aspects of biological reagents, biological agriculture and the like.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

Selenium-rich bacillus velezensis with strong tolerance and application thereof

The invention belongs to the technical field of microorganisms, and relates to a selenium-rich bacillus velezensis with high tolerance and application of the selenium-rich bacillus velezensis. The invention relates to a selenium-rich bacillus velezensis 915-1 with strong tolerance, which is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is (GDMCC No: 64021). The bacillus velezensis 915-1 can effectively inhibit growth of escherichia coli, salmonella and staphylococcus aureus after inoculation as first inoculation culture bacteria, and can be used as probiotics or drugs for colonization in intestinal tracts to play roles in inhibiting pathogenic bacteria and benefiting life. The bacillus velezensis is applied to production of organic selenium. The bacillus velezensis can absorb, convert and utilize sodium selenite, the sodium selenite is enriched into thalli to be converted into organic selenium, the content of the organic selenium is increased along with increase of the number of cells, and the bacillus velezensis can be used for production and preparation of the organic selenium.
Owner:GUANGXI VETERINARY RES INST +2

High-yield gastrodia elata planting method based on tilia brachypoda fungus material optimization and seed gastrodia elata stress resistance activation

The invention discloses a high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation. According to the high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation, yield increase and quality improvement are achieved through fungus material composite strengthening treatment and a composite strain synergistic inoculation technology. The method comprises the following steps: (1) fermenting a tilia brachypoda substrate by lactobacillus plantarum and soaking in a nano-composite nutrient solution to form a high-activity bacterial material; (2) nitrogen-fixing bacteria, disease-resistant actinomycetes and armillaria mellea are mixed and inoculated according to the proportion of 3: 1: 1, nitrogen supply is improved, and the occurrence rate of black rot is reduced; 3) activating a GASA stress-resistant gene of the seed hemp through a pulsed electric field (5 kV / cm, 10 microseconds), and enabling the germination rate to reach 88.5% in combination with a calcium signal channel strengthening technology; according to the method, the yield per mu of gastrodia elata is increased by 30%-40%, the gastrodin content is increased by 30% or above, the chemical pesticide dosage is reduced by 55%, the strain passage purity is stable, the method is suitable for large-scale planting in multiple climate areas, and an innovative solution is provided for green production of traditional Chinese medicinal materials.
Owner:INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI

Streptomyces strain EC15 and application thereof

The invention relates to a streptomyces sp. Strain EC15 and an application of the streptomyces sp. Strain EC15. The streptomyces strain EC15 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33195. The streptomyces strain EC15 has the advantages that the streptomyces strain EC15 can be used for preparing the streptomyces strain EC15; the strain has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing a biological membrane, producing an iron carrier, producing IAA and producing ACC deaminase. The EC15 bacterial liquid is used for soaking seeds under the stress of 200 mmol / L and 250 mmol / L NaCl, so that the germination potential, the germination rate, the germination index, the radicle length, the germ length and the fresh weight of group seedlings of the alfalfa seeds can be obviously improved. After the EC15 strain is inoculated, under the stress of 200 mmol / L NaCl, the plant height, stem diameter, leaf area and overground part fresh and dry weight of alfalfa plants can be obviously improved.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY

Poultry vaccination marking mechanism

The utility model discloses a poultry vaccination marking mechanism which comprises an ink jet mechanism, the ink jet mechanism is flat and hollow and is connected with an external liquid pump through a hose, one side of the ink jet mechanism is provided with a nozzle, the other side of the ink jet mechanism is rotatably provided with a limiting ball, one end of the limiting ball is connected with a connecting mechanism through a connecting rod, and the other end of the limiting ball is connected with the connecting mechanism. The input end of the liquid pump is connected with an ink box containing marking ink through a pipeline, and the output end of the liquid pump is connected with the ink jetting mechanism through a hose. The trigger switch of the liquid pump can be linked with the vaccine injector, when the vaccine injector is started to inject vaccines once, the liquid pump operates once, a certain amount of ink is sprayed to feathers on poultry bodies, and the ink-jet mechanism is linked with the vaccine injection mechanism to inject the vaccines into the feathers. Meanwhile, the poultry feather marking device is small and exquisite in design, light, simple in structure, low in use and maintenance cost and suitable for small-scale poultry breeding.
Owner:JIANGXI XINJISEN POULTRY IND CO LTD

Novel efficient biological cultivation equipment for black land based on earthworm-driven inoculation

The invention discloses novel efficient biological cultivation equipment for black land based on earthworm driven inoculation, and designs novel pure-electric-driven unmanned earthworm complex large-field throwing equipment in order to solve the problem that existing throwing machinery for earthworm complexes is lacked. According to the device, mixed liquid acts on materials at the bottom of the material box in a spraying mode, the overhead phenomenon formed in the material box due to adhesion of the materials is avoided, and it is guaranteed that the materials are continuously and fully filled into the discharging mechanism; according to the feeding device with the earthworm feeding hopper and the straw mixture feeding hopper as main components, accurate feeding is achieved under the condition that the reasonable sowing amount is controlled, and the feeding distance and the feeding amount can be controlled according to actual needs. According to the design, the requirement of intelligent agricultural machinery is met, quantitative and damage-free putting of the earthworm complex is met, and a feasible solution is provided for solving the problems of insufficient soil porosity and soil hardening.
Owner:JILIN UNIVERSITY

Combined flora YB2 capable of degrading PET (Polyethylene Terephthalate) plastic and application of combined flora YB2

The invention discloses a combined flora YB2 capable of degrading PET (Polyethylene Terephthalate) plastic and application of the combined flora YB2, and belongs to the technical field of microorganisms. The combined flora YB2 is composed of Burkholderia cepacia ZY1 and Pseudomonas hardendinii G1B, the optimal inoculation ratio of the Burkholderia cepacia ZY1 to the Pseudomonas hardendinii G1B is 4%: 5%, and the optimal growth conditions are that the pH is 7.0 and the temperature is 30 DEG C. The combined flora YB2 not only can effectively degrade the PET film, but also can effectively degrade PET intermediate products BHET, MHET and TPA. The weight loss ratio of the PET film treated by the combined flora YB2 is 4.3%, obvious cracks and pores are formed in the surface of the PET film, key functional groups C-O, C = O and C-H of the PET film are obviously reduced, and a new solution is provided for efficient degradation of PET.
Owner:FUZHOU UNIV

Intelligent vaccine supply chain collaborative optimization system based on multi-source data fusion

The invention provides an intelligent vaccine supply chain collaborative optimization system based on multi-source data fusion, and relates to the technical field of vaccine supply chains, and the system comprises a data acquisition and processing module which is used for collecting and preprocessing multi-source heterogeneous data in real time; the intelligent prediction and optimization module is used for constructing a hybrid prediction model and predicting vaccine demands; the storage and logistics management module is used for constructing a virtual warehouse model; the collaborative decision module is used for generating a multi-dimensional supply chain scheduling scheme and evaluating the feasibility and efficiency of the scheme; and the dynamic priority marking module is used for carrying out priority ranking on vaccines. By constructing the hybrid prediction model, the future vaccine demand can be accurately predicted, the prediction accuracy and reliability are improved, vaccine shortage or excess can be avoided, and the response speed and flexibility of a supply chain are improved; through accurate priority ranking of vaccines, when emergency situations such as epidemic situations and the like are coped, resources can be quickly allocated, and the inoculation requirement is met to the maximum extent.
Owner:HUBEI PROVINCIAL CENT FOR DISEASE CONTROL & PREVENTION (HUBEI ACAD OF PREVENTIVE MEDICINE)

Tremella liquid strain inoculation device

The utility model relates to a tremella liquid strain inoculation device which comprises a rack, a conveying roller mechanism used for transversely conveying a culture bottle tray is arranged on the rack, a first station and a second station are sequentially arranged on the conveying roller mechanism in the conveying direction, a first blocking mechanism used for blocking the tray is arranged on the first station, and a second blocking mechanism used for blocking the tray is arranged on the second station. A drilling mechanism which can ascend and descend and is used for enabling a culture medium in the culture bottle to form a concave hole is arranged above the first station, a second blocking mechanism which is used for blocking the tray is arranged on the second station, and a bacterium injection mechanism which can ascend and descend and is used for injecting a liquid strain into the concave hole of the culture medium is arranged above the second station. The device is compact in structure, inoculation of liquid strains in the tremella culture bottle can be conveniently achieved, and the liquid strains can enter a culture medium more quickly.
Owner:FUJIAN XIANGYUN BIOTECH DEV CO LTD

Method for preparing organic selenium by using saussurea involucrata and application of organic selenium

PendingCN120966925AFermentationFood scienceBiotechnologySaussurea involucrata
The invention discloses a method for preparing organic selenium from saussurea involucrata and application of the method, and belongs to the technical field of organic selenium preparation. The preparation method of the organic selenium provided by the invention comprises the following steps: the inoculation amount of saussurea involucrata is 2.5%, a sterile Na2SeO3 solution is added after culture is performed for 2 hours, the working concentration of Na2SeO3 is 25 mu g / mL, and culture is continued for 36 hours after uniform mixing; the organic selenium is successfully prepared by adopting the preparation method provided by the invention, the final total selenium content is 1356.60 + / -23.75 mu g / g, the selenium conversion rate is 19.94 + / -0.14%, the organic selenium content is 1177.74 + / -28.82 mu g / g, and the proportion is 86.81 + / -0.89%; the provided preparation method of the selenium-rich saussurea involucrata bacteria is simple and easy to operate, has low requirements on instruments and equipment and high conversion rate of organic selenium, and can be used for industrial production of organic selenium.
Owner:NORTHWEST UNIV

Schizochytrium limacinum with high DHA content and low DPA content and application of schizochytrium limacinum

The invention belongs to the field of microbial genetic engineering, and provides schizochytrium limacinum with high DHA content and low DPA content and application of the schizochytrium limacinum. According to the strain, an n3 front-end desaturase gene derived from crypthecodinium cohnii is integrated to a 18S rDNA site of schizochytrium limacinum ALS01, and omega-6 type DPA is converted into DHA by utilizing an extension-desaturation way. Under the optimized conditions of a 5L fermentation tank (the inoculation amount is 5-20%, the temperature is 25-28 DEG C, the ventilation ratio is 1.0-2.0 vvm, and fermentation is performed for 72-168 hours), the biomass is larger than or equal to 150g / L, the total grease is larger than or equal to 60%, the DHA total grease proportion is larger than or equal to 53%, the DPA total grease proportion is smaller than or equal to 2%, and the DHA yield is larger than or equal to 47.82 g / L. The invention solves the problems of unstable fish oil resource and insufficient DHA purity in the traditional DHA production, provides a new strain for the industrial production of high-purity DHA, and has important application value.
Owner:HANG ZHOU HE TAN CHUANG WU KE JI YOU XIAN GONG SI +1

Wehonor coccus and application thereof in relieving subacute rumen acidosis of ruminants

The invention belongs to the technical field of microorganisms, and particularly relates to a Wehonor coccus strain and application thereof in relieving subacute rumen acidosis of ruminants. The invention relates to a Villonella sp. Strain RGHV1, the Villonella sp. Strain is preserved in China General Microbiological Culture Collection Center on January 21, 2025, and the preservation number of the Villonella sp. Strain is CGMCC NO.33487. The Villonella sp. Strain is a Villonella sp. Strain RGHV1. The Villonella sp. Strain is a Villonella sp. Strain RGHV1. The Villonella sp. Strain RGHV1. The Wehonor coccus RGHV1 is separated from the rumen fluid of Hu sheep, the strain name is undefined and is not fully researched before, and the research on the strain finds that the Wehonor coccus RGHV1 has the characteristics of fast growth and strong lactic acid degradation capacity, can metabolize lactic acid to generate acetic acid and propionic acid, and has the advantages of high yield, high yield and the like. By inoculating the low-concentration strain, the lactic acid level in in-vitro fermentation rumen fluid can be obviously reduced, the generation of VFA (Vitamin Fatty Acid) is promoted, and compared with the peste des petits ruminants subsp.lactis, the peste des petits ruminants subsp.lactis has stronger capability of reducing rumen lactic acid.
Owner:ZHEJIANG UNIV

Salt-tolerant bacillus Bah-38 strain and application thereof

The invention provides a salt-tolerant bacillus strain Bah-38, the classification name of the salt-tolerant bacillus strain Bah-38 is the salt-tolerant bacillus strain Bah-38, the salt-tolerant bacillus strain Bah-38 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the salt-tolerant bacillus strain Bah-38 is CGMCC NO.33642. The invention further provides a preparation method of the salt-tolerant bacillus strain Bah-38. According to the invention, a confrontation culture test proves that the Bah-38 strain has a bacteriostatic effect on fusarium solani, and the inhibitory effect of the strain on the fusarium solani reaches 51.4%. In a potting inoculation test, the prevention and control effect on the root rot caused by fusarium solani reaches 70.6% after the fermentation liquor of the strain is subjected to root irrigation treatment. Meanwhile, the obvious growth promoting effect is achieved. The fusarium solani has a high and stable control effect on fusarium solani, and is suitable for agricultural production requirements.
Owner:INSTITUTE OF VEGETABLES & FLOWERS CHINESE ACADEMY OF AGRICULTURAL SCIENCES

Auxiliary inoculation device for animal husbandry and veterinary medicine

The invention discloses an inoculation auxiliary device for animal husbandry and veterinary, and relates to the technical field of animal husbandry, the inoculation auxiliary device comprises a cart, the surface of the cart is fixedly provided with a first fixing frame, the top of the first fixing frame is fixedly provided with a hydraulic cylinder, the surface of the cart is rotatably provided with a limiting plate, and the surface of the cart is fixedly provided with a placing clamping plate; a first fixing frame is fixedly installed on the surface of the cart, a second fixing frame is fixedly installed on the surface of the cart, a clamping plate is slidably installed on the surface of the first fixing frame, and a connecting plate is fixedly installed on the surface of the clamping plate. At the moment, the telescopic arc plate moves downwards to press and fix the body of the livestock, so that the injection operation is more accurately carried out, the stability of the body position of the livestock is ensured, the situation that a needle enters a wrong position by mistake due to animal movement is reduced, and the success rate and the effect of inoculation are improved.
Owner:ZHENJIANG ZHONGHANG INTELLIGENT TECHNOLOGY CO LTD