181 results about "Fusarium solani" patented technology

The invention relates to the technical field of microorganisms, and discloses burkholderia gladioli as well as a bacterial agent and application of the burkholderia gladioli. The Burkholderia gladioli (Burkholderia gladioli) CCTCC (China Center For Type Culture Collection) NO: M20242288 provided by the invention has good capacities of fixing nitrogen, dissolving phosphorus, dissolving zinc, producing siderophores and oxidizing sulfur, and can be used for regulating the nutrient structure of soil and converting substances which are difficult to utilize by plants in the soil, so that the growth of the plants is promoted, and the stress resistance of the plants is improved; the strain also can be used for antagonizing Fusarium solani, Fusarium equiseti, Fusarium verticillium, Fusarium verticillium and Rosellinia necatrix, can be used for antagonizing Fusarium solani, Fusarium equiseti, Fusarium verticillium and Rosellinia necatrix, and can also be used for antagonizing Fusarium solani, Fusarium equiseti, Fusarium verticillium, Rosellinia necatrix, Fusarium moniliforme and Rosellinia necatrix, Fusarium moniliforme and Rosellinia necatrix. Besides, the burkholderia gladioli provided by the invention is a strain separated from rape farmland soil, and compared with a strain screened by extreme environment and laboratory mutagenesis, the burkholderia gladioli has better adaptability when being applied to a planting environment, and can be stably colonized in plant rhizosphere and soil, so that the burkholderia gladioli stably plays a growth promoting role for a long time, is safe and environment-friendly, and has a wide application prospect. Wide application prospects are realized.

The invention relates to the technical field of microorganisms, and discloses trichoderma yunnanense and application thereof. The Trichoderma yunnanense CCTCC NO: M 2025645 provided by the invention is a dominant strain of a high-yield siderophore, the activity of the siderophore is 69.79%, and the Trichoderma yunnanense can be used for synthesizing and secreting carboxylic acid type siderophore; the strain has a sulfur oxidation function; in addition, the invention also provides an application of the trichoderma sp. In regulation and control of plant diseases, the trichoderma sp. Disclosed by the invention can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae (Fr.) Kissler, and the trichoderma sp. Can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae (Fr.) Kissler, and can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae. The Trichoderma yunnanense provided by the invention is a strain separated from tobacco planting soil, and compared with a strain subjected to mutagenesis screening in an extreme environment and a laboratory, the Trichoderma yunnanense has better adaptability when being applied to a planting environment, and can be stably colonized in plant rhizosphere and soil, so that the Trichoderma yunnanense stably plays a growth promoting role for a long time, is safe and environment-friendly, and has a wide application prospect.

The invention discloses a Burkholderia gladioli (Burkholderia gladioli) CT-5 strain and an application of the Burkholderia gladioli CT-5 strain, and relates to the technical field of microorganisms. The strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M20242732, and the preservation date is December 05, 2024. The Burkholderia gladioli (Burkholderia gladioli) CT-5 provided by the invention has a remarkable prevention and treatment effect (Plt, Plt) on idesia root rot caused by Fusarium solani (Fusarium solani), and the Burkholderia gladioli (Burkholderia gladioli) CT-5 has a remarkable prevention and treatment effect on idesia root rot caused by Fusarium solani (Busarium solani). The compound has the advantages that the compound can be used for inhibiting pathogenic bacteria of various plants, and the compound can be used for inhibiting pathogenic bacteria of various plants, such as Schizophyllum commune, Diaporthe erens, Colletotrichum acutatum and the like, and has a remarkable inhibiting effect on other various plant pathogenic bacteria such as the Schizophyllum commune, the Diaporthe erens, the Colletotrichum acutatum and the like. In a pot experiment, the biomass, soil nutrients, SOD, POD and CAT enzyme activities of idesia polycarpa seedlings in a CT-5 treatment group are remarkably improved compared with those of a pathogenic bacteria control group (FP) (Plt; 0.05), which indicates that the strain can promote plant growth and activate the activity of defensive enzyme in the body, so that effective control of idesia root rot is realized, and powerful theoretical basis and technical support are provided for sustainable development of idesia.

The invention discloses a pseudomonas synthetic flora and application thereof in prevention and treatment of apple continuous cropping obstacles, and belongs to the technical field of agricultural microorganisms. The synthetic flora is constructed from three strains of pseudomonas, i.e., alkylphenol pseudomonas, oleaginous pseudomonas and pseudomonas putida, and the biological preservation numbers of the three strains of pseudomonas are respectively as follows: alkylphenol pseudomonas S4, CGMCC (China General Microbiological Culture Collection Center) No.36078; the culture medium is characterized in that the culture medium is Pseudomonas oleovorans S38, and the culture medium is CGMCC (China General Microbiological Culture Collection Center) No.36079; pseudomonas putida S42, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.36080, is CGMCC No.36080. The synthetic flora has a relatively strong antagonistic effect on main pathogenic bacteria (fusarium oxysporum and fusarium solani) of apple continuous cropping obstacles, and the inhibition capability of the synthetic flora is remarkably superior to that of a single strain; field and pot experiment results show that the liquid bacterial fertilizer and the solid bacterial fertilizer of the synthetic flora can promote the growth of overground parts and underground parts of the continuous cropping apple trees; the number of harmful fungi in continuous cropping soil is obviously reduced; the root protecting enzyme activity is improved, and the MDA content is reduced; the rhizosphere micro-ecology of the continuous cropping apples is optimized, and the continuous cropping obstacles of the apples are remarkably relieved.

The invention discloses talaromyces purpurogenum as well as a fermented supernatant and application thereof. The preservation number of the talaromyces purpureogenus F-4 is CCTCC NO: M 20252023, and the talaromyces purpureogenus F-4 has the growth promoting effects of dissolving phosphorus, fixing nitrogen and generating siderophores, also has the disease preventing and treating effects of preventing and treating garlic root rot, potato scab and wheat stem rot, and also can resist salt stress, alkali stress, salt-alkali composite stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the talaromyces purpureus F-4 can be used for inhibiting the growth of fusarium solani spores, streptomyces botrytis spores and fusarium pseudograminearum and the germination of the spores of the fusarium solani, the streptomyces botrytis and the fusarium pseudograminearum. The talaromyces purpurogenus and the fermented supernatant thereof can effectively control the growth and reproduction of plant pathogens, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, and reduce the risk of environmental pollution and agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention discloses a Brucella with antibacterial activity and application of the Brucella. The invention discloses Burkholderia sp.HNIV0701, and the preservation number of the Burkholderia sp.HNIV0701 is GDMCC No: 66007. The Burkholderia sp.HNIV0701 disclosed by the invention has a stable, efficient and broad-spectrum antibacterial property, has an inhibition effect on a plurality of plant pathogenic bacteria such as Botryosphaeria dothidea, Phytophthora nicotianae, Fusarium solani, Alternaria alternata, Thanatepora cucurbitae, Ralstonia solanacearum and the like, and also can be used for inhibiting Staphylococcus aureus and / or Listeria monocytogenes, so that the Burkholderia sp.HNIV0701 can be used for inhibiting a plurality of plant pathogenic bacteria such as the Botryosphaeria dothidea, the Phytophthora nicotianae, the Fusarium solanum, the Alternaria solanacearum and the like. The Brucella HNIV0701 can hydrolyze organic phosphorus and can be used for soil improvement. The Brucella HNIV0701 has nitrogen fixation capability and has the potential of promoting plant growth.

The invention relates to a microbial agent for preventing and treating root rot of radix tinosporae and application of the microbial agent. The microbial agent is prepared from the following components: bacillus velezensis with the preservation number of CCTCC (China Center for Type Culture Collection) No: M2022923, bacillus subtilis with the preservation number of CCTCC No: M20221207, a marigold extract, amino acid, humic acid and medium trace elements. When the microbial agent is applied to the planting process of radix tinosporae, the physicochemical property of radix tinosporae planting soil can be remarkably improved, the enzyme activity and the microbial diversity can be improved, the content of phenolic acid and fusarium toxin in the soil can be reduced, the disease index of radix tinosporae root rot caused by fusarium solani and pythium aphanidermatum can be further reduced, and finally prevention and control of radix tinosporae root rot can be achieved.

The content of IAA produced by the pantoea agglomerans JHS0317 can reach 26.81 mg / L, the content of ACC deaminase produced by the pantoea agglomerans JHS0317 is 470.34 U / L, the pantoea agglomerans JHS0317 has multiple functions of dissolving phosphorus, dissolving potassium, fixing nitrogen, producing siderophore and the like, meanwhile, the pantoea agglomerans JHS0317 has inhibition effects on tobacco fusarium oxysporum and fusarium solani, the inhibition rates are 42.98% and 41.20%, the germination rate of tobacco seeds is increased through treatment of the strain JHS0317, and the root length is remarkably promoted; the root activity, the whole plant fresh weight, the overground fresh weight, the root fresh weight, the maximum leaf length and the maximum leaf width of tobacco seedlings treated by the strain JHS0317 with the bacterium content of 1 * 10 < 5 > cfu / mL in a seedling culture substrate are obviously higher than those of the tobacco seedlings treated by a contrast, and the strain can be used as a plant growth-promoting rhizobacteria and has a good application prospect in research and development of microbial fertilizers and biological prevention and control of tobacco fusarium root rot.

The invention discloses bacillus siamensis capable of antagonizing various phytopathogens and application of the bacillus siamensis. The preservation number of the bacillus siamensis A-2 is GDMCC (China General Microbiological Culture Collection Center) NO.64228. The bacillus siamensis A-2 has the advantages that the preservation number of the bacillus siamensis The strain has an obvious inhibition effect on Releasella syzygium jambos Indian niesia subspecies, pseudomonas solanacearum, fusarium solani, phomopsis, fusarium oxysporum, colletotrichum musae, colletotrichum laurosporum, fusarium fujikubin and fusarium sp. The gene clusters contain gene clusters for synthesizing plipastatin, abinogen, surfactin, siderophore, bacitracin, difixitin, butyrosidin A / B and some terpene compounds, and the gene clusters contain gene clusters for synthesizing plipastatin, abinogen, surfactin, siderophore, bacitracin, difixitin and butyrosidin A / B; the composition has a relatively good prevention and treatment effect (the relative prevention effect is 58.14%) on tomato bacterial wilt; and the strain has the capability of degrading tetracycline hydrochloride (the degradation rate in 3 days is 49.28%). Therefore, the bacillus siamensis A-2 has extremely high development and utilization value in agricultural production.

The invention relates to the technical field of biology, in particular to application of PnPUB35 protein and a coding gene thereof to regulation and control of plant wilt resistance. The invention provides a Ubiquitin ligase PnPUB35 protein containing a U-box structural domain E3 and a gene PnPUB35 of the Ubiquitin ligase PnPUB35 related to resistance to fusarium wilt of pepper, and after the pepper is inoculated with fusarium solani, the expression quantity of the Ubiquitin ligase PnPUB35 protein at the root of the pepper is increased. The resistance of a pepper PnPUB35 silent plant to fusarium solani is weakened, and the resistance of an arabidopsis thaliana PnPUB35 overexpression plant to fusarium solani is enhanced. The PnPUB35 gene disclosed by the invention plays a positive regulation role in pepper wilt resistance, and can be used as an important candidate gene for pepper wilt resistance breeding.

The invention relates to the technical field of phytopathogen detection, and particularly discloses a primer, a kit and a method for detecting crop root rot fusarium solani and application, the primer comprises a specific RPA primer RPA-F and a specific RPA primer RPA-R; the specific RPA primer RPA-F is as shown in SEQ ID NO. 1, and the specific RPA primer RPA-R is as shown in SEQ ID NO. 2; the kit further comprises a guide RNA primer crRNA, and the guide RNA primer crRNA is as shown in SEQ ID NO. 3. The kit comprises a specific RPA primer RPA-F, a specific RPA primer RPA-R, a Cas12 protein, a T7 transcriptase, a crRNA DNA template, a DNA polymerase and a Cas12 / 13 nucleic acid detection test strip. The kit has the characteristics of good sensitivity, relatively good specificity and enough reliability.

The content of IAA produced by the enterobacter ludwigii XL0404 can reach 18.94 mg / L, the content of ACC deaminase produced by the enterobacter ludwigii XL0404 is 484.48 U / L, and the enterobacter ludwigii XL0404 has multiple functions of dissolving phosphorus, dissolving potassium, fixing nitrogen, producing siderophore and the like, has an inhibition effect on main root and stem disease pathogenic bacteria such as phytophthora nicotianae, rhizoctonia solani, rhizoctonia tectorum, fusarium oxysporum, fusarium solani and the like, and has an inhibition rate of 41.20-55.88%; the germination rate of the tobacco seeds is increased through treatment of the strain XL0404, and the root length is remarkably promoted; the root activity, the whole plant fresh weight, the overground fresh weight, the root fresh weight, the maximum leaf length and the maximum leaf width of the tobacco seedlings treated by the strain XL0404 with the bacterium content of 1 * 10 < 7 > cfu / mL in a seedling culture substrate are obviously higher than those of the tobacco seedlings treated by contrast treatment, and the strain XL0404 can be used as plant growth-promoting rhizobacteria and has a good application prospect in research and development of microbial fertilizers and biological prevention and control of tobacco rhizome diseases.

The invention discloses radix ophiopogonis endophytic fungi and application thereof, and belongs to the technical field of plant disease prevention and control. The endophytic fungi are separated from liliaceae ophiopogon plant ophiopogon japonicus living bodies by adopting an endophytic fungi separation and purification technology, belong to xylaria, xylaria and carbendazim fungi, are classified and named as Nemania diffusa OJ51, and have a strain preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 41084. The endophytic fungus can inhibit mycelial growth of pathogenic bacteria, namely Fusarium oxysporum and Fusarium solani, of radix ophiopogonis root rot, the relative inhibition rates are 63.51 + / -1.22% and 66.27 + / -1.03% respectively, meanwhile, the disease condition of radix ophiopogonis root rot is effectively relieved, and the disease index is reduced by 76.70%. The endophytic fungus has an obvious effect of preventing and treating radix ophiopogonis root rot, and a wide application prospect is brought to the field of biological prevention and treatment of radix ophiopogonis fungus diseases.

The invention discloses a penicillium fungus GS218, a penicillium fungus GS218 conidium suspension and a preparation method and application of the penicillium fungus GS218 conidium suspension. The penicillium fungus GS218 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC M 20241477, and the penicillium fungus GS218 is separated from fruits of trifoliate orange in a Hunan Institute of Gardening Institute of Hunan Province in the East Lake Street, Hibiscus District, Changsha City, Hunan Province. The penicillium fungus has a relatively good antagonistic effect on HLB13 fusarium buchneri, colletotrichum spinosus, fusarium solani, fusarium oxysporum cucumber specialized type and botrytis cinerea; the composition can be used for effectively preventing and treating carrot diseases caused by HLB13 fusarium brucellosis, pepper anthracnose caused by colletotrichum spinosus, pepper root rot caused by fusarium solani, cucumber fusarium wilt caused by fusarium oxysporum cucumber specialization and botrytis cinerea caused by botrytis cinerea, and has remarkable and stable technical effects; the method has wide application value in the technical field of microbial strain application.

The invention relates to the technical field of biology, in particular to application of glucoside hydrolase FsGH28c and a coding gene thereof in regulation of strain pathogenicity. The invention provides a glucoside hydrolase coding gene FsGH28c related to development, carbon source utilization and pathogenicity of fusarium solani, and after the FsGH28c gene is knocked out, the sporulation quantity is reduced, spore development is deformed, and the utilization rate of a carbon source (especially pectin) is reduced. In addition, the colonization amount of the FsGH28c gene deletion mutant in host pepper root fungi is reduced, and the pathogenicity is reduced. The FsGH28c gene can positively regulate the pathogenicity of fusarium solani, and can be used as an important candidate gene for research on the pathogenesis of fusarium solani.

The present invention provides a composite bacterial community for degrading polycyclic aromatic hydrocarbons and its application, belonging to the field of microbial technology. The composite bacterial community of the present invention comprises Fusarium solani E1 and Rhodococcus erythroxysporum E2; the preservation number of Fusarium solani E1 is CGMCC No: 41063, and the preservation number of Rhodococcus erythroxysporum E2 is CGMCC No: 29266. The present invention combines fungi and bacteria to synergistically degrade PAHs in the repair of PAHs. The composite bacterial community has better PAHs degradation effect and composite bacterial growth status than single bacteria, and has good effects on the degradation of high- and low-ring PAHs.

The present invention relates to methods for controlling or preventing infestation of vegetable, tomato and potato plants by phytopathogenic microorganisms selected from Sphaerotheca fuliginea, Leveillula taurica, Sclerotinia sclerotiorum, Cercospora, Fusarium oxysporum, Fusarium solani, Helminthosporium solani, Phoma tuberosa, Rhizoctonia solani, Phytophthora infestans, Verticillium dahlia, Didymella bryoniae, Botrytis cinerea and Alternaria solani, comprising applying to a crop of plants, the locus thereof, or propagation material thereof, a compound according to formula (I) wherein R1, R2, R3, R4, R5, Y, A, B are as defined herein.

The invention discloses bacillus velezensis and application thereof in selenium enrichment of crops and inhibition of disease bacteria, and belongs to the technical field of selenium-enriched biocontrol bacteria. The bacillus velezensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus velezensis is CGMCC No.34490. The bacillus velezensis is named as bacillus velezensis. The strain has efficient selenium-rich conversion capability, and can convert inorganic selenium (such as sodium selenite) with relatively high toxicity into organic selenium or nano-selenium particles with low toxicity or no toxicity and high bioavailability. In addition, the strain also has a better antifungal effect, and has an excellent inhibition effect on fungi such as fusarium solani, arbuscular oryzae, fusarium equiseti, fusarium oxysporum and the like, so that the purpose of biological prevention and control can be achieved, plant diseases can be prevented, and healthy growth of plant crops is facilitated.

The invention discloses a marine fungus fusarium keratinoides and application thereof. The fusarium keratinoides capable of efficiently producing the perindoprine is screened from marine sediments, and the content of the produced perindoprine can reach 561.7 mu g / L after the fusarium keratinoides is fermented in a GPY liquid culture medium, so that possibility and a foundation are provided for breeding strains suitable for industrial large-scale production of the perindoprine in the future; mass spectrum, hydrogen spectrum and carbon spectrum data of the separated perindoprine are completely consistent with perindoprine data reported in literatures.

The invention provides a salt-tolerant bacillus strain Bah-38, the classification name of the salt-tolerant bacillus strain Bah-38 is the salt-tolerant bacillus strain Bah-38, the salt-tolerant bacillus strain Bah-38 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the salt-tolerant bacillus strain Bah-38 is CGMCC NO.33642. The invention further provides a preparation method of the salt-tolerant bacillus strain Bah-38. According to the invention, a confrontation culture test proves that the Bah-38 strain has a bacteriostatic effect on fusarium solani, and the inhibitory effect of the strain on the fusarium solani reaches 51.4%. In a potting inoculation test, the prevention and control effect on the root rot caused by fusarium solani reaches 70.6% after the fermentation liquor of the strain is subjected to root irrigation treatment. Meanwhile, the obvious growth promoting effect is achieved. The fusarium solani has a high and stable control effect on fusarium solani, and is suitable for agricultural production requirements.

The invention discloses bacillus subtilis S1703 and application thereof, and belongs to the technical field of plant disease and pest control. The name of the bacterial strain is bacillus subtilis S1703, and the preservation number of the bacterial strain is GDMCC No: 66890. The bacterial strain S1703 has a wider antibacterial spectrum, and can effectively inhibit the growth of pathogenic bacteria such as fusarium oxysporum, colletotrichum gloeosporioides, alternaria alternata, aspergillus, phyllosticta, citrus reticulata, fusarium and the like; fermentation liquor of the S1703 has a good antibacterial effect on the psidium guajava wilt pathogen and the psidium guajava branch blight pathogen; the bacteriostasis rate on sigatoka sigatoka and guava leaf spot germs reaches 95% or above, and an excellent bacteriostasis effect is achieved. The strain S1703 can avoid potential adverse effects of chemical pesticides on the environment and crop safety, and has potential commercial development and application values in biological prevention and control practice of diseases.

The invention belongs to the technical field of plant cultivation, and particularly relates to a screening and fermentation method of a pepper root rot antagonistic bacterium W-5, which comprises the following steps: 1, preparing experimental materials, namely preparing a test strain, a culture medium, a reagent and an instrument; 2, separating, screening and identifying the antagonistic bacteria; 3, observing the influence of the antagonistic bacteria W-5 on the growth of the mycelia at the front end of the fusarium solani; step 4, detection of pathogenic fungus bacteriostasis spectrum by antagonistic bacteria W-5, the structure is reasonable, the main pathogenic fungus fusarium solani causing the pepper root rot disease is taken as an indicator bacterium, the pepper root rot antagonistic bacteria are obtained through separation by adopting a soil plate dilution method and a plate confrontation method, and the antagonistic bacteria are identified by combining morphology with molecular biology; meanwhile, the broad-spectrum antibacterial activity of the strain W-5 is measured, and the optimal fermentation condition of the strain W-5 is optimized based on a response surface method designed by combining a single-factor test with Box-Behnken, so that ginseng is expected to be provided for development and application of a pepper root rot antagonistic biological agent.

The invention provides a bacillus thuringiensis L16 strain and application thereof, and relates to the technical field of agricultural biological control. The bacillus thuringiensis L16 strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on October 23, 2024, and the preservation number is GDMCC NO.65334. The strain is a gram-positive bacterium, can generate protease, amylase and cellulase, can decompose potassium and fix nitrogen, and has the advantages of high yield, low cost, high yield and high yield. The strain can effectively inhibit the growth of root rot pathogenic bacteria fusarium solani and fusarium oxysporum, can promote the growth of the chrysanthemum, and provides an excellent biocontrol strain for preventing and treating continuous cropping obstacles of the chrysanthemum and promoting the growth of the chrysanthemum; the method is of great significance to green prevention and control technology of Chuzhou chrysanthemum diseases and promotion of sustainable development of the Chuzhou chrysanthemum industry.

The invention discloses a streptomyces albidoflavus strain SaTC2601 and application thereof, and belongs to the field of biological control. According to the invention, a streptomyces albidoflavus strain SaTC2601 is separated from faeces of larvae of WSFC, and the preservation number of the streptomyces albidoflavus strain SaTC2601 is CGMCC (China General Microbiological Culture Collection Center) No.33741. The strain SaTC2601 has a very good prevention and treatment effect on peanut southern blight caused by sclerotium rolfsii at peanut roots, also has a prevention and treatment effect on soil-borne pathogenic bacteria such as fusarium graminearum, fusarium solani, fusarium moniliforme, fusarium oxysporum, rhizoctonia solani and streptomyces scabies, and further, the strain SaTC2601 also has a very good growth promoting effect. The strain becomes a precious resource for new drug development and agricultural disease prevention and control, and is expected to play a huge role in the aspect of soil disease prevention and control.

The invention relates to the field of agricultural biotechnology and plant protection, and particularly provides application of 2-heptanone to preparation of a preparation for preventing and treating plant diseases. The bactericidal composition has a strong bacteriostatic effect on litchi colletotrichum gloeosporioides, strawberry colletotrichum gloeosporioides, mango leaf blight, pawpaw colletotrichum gloeosporioides, wheat gibberellic disease, mango colletotrichum gloeosporioides, tomato fusarium wilt, banana fusarium wilt, banana colletotrichum gloeosporioides and corn root rot. The fumigant developed on the basis of 2-heptanone and used for preventing and treating phytopathogen is expected to be used for preventing and treating compound diseases caused by various pathogenic bacteria, or comprehensively preventing and controlling different crop diseases in a crop rotation system, and has important development value.

The invention provides a vanillin compound spliced by polyheterocycles. The polyheterocycles are one of a benzimidazole ring, a thiadiazole ring, an oxadiazole ring and a thiazole ring; the vanillin compound spliced by polyheterocycles is one of vanillin derivatives containing a benzimidazole ring, a thiadiazole ring, an oxadiazole ring and a thiazole ring, and the chemical general formula is shown in the specification. The polyheterocycle spliced vanillin compound is prepared through a condensation reaction and a condensation-cyclization reaction, and the compound has good antibacterial activity on valsa mali, red kidney bean root rot, fusarium graminearum, millet pyricularia grisea, botrytis cinerea, alternaria solani and watermelon fusarium oxysporum. The compound can be used for preparing a novel efficient antibacterial agent and is applied to prevention and treatment of the pathogenic bacteria.

The present invention relates to the field of microorganisms, and particularly to a Bacillus amyloliquefaciens BZWQ833, a microbial agent and its application. A Bacillus amyloliquefaciens was screened from the rhizosphere soil of a potato common scab field with continuous cropping for many years, named Bacillus amyloliquefaciens BZWQ833 and subjected to biological preservation. This bacterium is a Gram-positive bacterium that can produce spores and can produce protease, cellulase and pectinase. The present invention first discovers that Bacillus amyloliquefaciens BZWQ833 can inhibit the growth and incidence of Streptomyces scabies, Rhizoctonia solani, Fusarium oxysporum and Fusarium solani, and the antibacterial effect is stable. Moreover, through greenhouse pot experiments and field control experiments, it is verified that Bacillus amyloliquefaciens BZWQ833 has a significant control effect on various plant soil-borne diseases (common scab, powdery scab and fruit rot), providing a new biological control resource for the biological control of plant soil-borne diseases.

A microbial composite strain H2, antagonistic to multiple potato pathogens, and its application and preparation method, relates to the field of biological control. The microbial composite strain H2 is a mixture of Bacillus subtilis, Bacillus atrophaeus, and Bacillus Widmannii. It exhibits strong inhibitory effects against potato early blight pathogens Alternaria solani and Alternaria alternata; wilt / dry rot pathogens Fusarium solani, Fusarium oxysporum, and Fusarium equiseti; and leaf / stem spot pathogen Vitis vinifera, with inhibition rates exceeding 46%. The highest inhibition rate against Alternaria alternata reached 75.97%. H2 also exhibits significant growth-promoting and yield-increasing effects, providing a high-quality strain for the comprehensive control of soil-borne potato diseases and promising prospects for field application.

The application discloses a molecular marker, primer, method and application for identifying tobacco root rot Fusarium proliferatum, and belongs to the technical field of plant disease molecular detection. The molecular marker is a nucleotide sequence shown in SEQ ID NO. 1, and can accurately distinguish tobacco root rot Fusarium proliferatum from its close species, i.e., Fusarium solani, Fusarium equiseti and Fusarium oxysporum. The specific primer pair based on the marker is composed of FPITS-3F (5'-GCTTGCCGCAAGGGCT-3') and FPITS-3R (5'-AAGGTCTCCGTTGGTGAACC-3'). The target bacteria can be rapidly identified by using the primer pair to perform PCR amplification and detecting whether a 396 bp characteristic band exists in the amplification product. The primer has high specificity and has no cross reaction to the above close species. The sensitivity is high, and the lower limit of detection of genomic DNA is 1*10-3 ng*muL-1, and the spore detection sensitivity in soil is 1 CFU / g. The method is simple in operation, and the detection can be completed in 2 hours only by using a common PCR instrument, and is suitable for early rapid diagnosis, monitoring and early warning in laboratories and production lines.

The invention discloses a serratia pumila strain for preventing and treating soybean root rot and application of the serratia pumila strain. The serratia pumila strain G25-16 is separated from rhizosphere soil of soybeans and wheat, and the microbial preservation number of the serratia pumila strain G25-16 is CGMCC (China General Microbiological Culture Collection Center) No.34772; a plate confrontation experiment result shows that the strain has an obvious antagonistic effect on fusarium solani and fusarium malodosa; a control effect experiment result shows that the strain has a remarkable control effect on fusarium solani and fusarium malodosa; potted plant growth promotion experiments find that after soybeans are treated by the separated serratia pumila strain, the fresh weight and plant height of the soybeans are obviously improved compared with those of a contrast. The serratia pumila strain G25-16 separated by the invention has application prospects in the aspects of promoting soybean growth, preventing and treating soybean diseases and the like.