43 results about "Lilium regale" patented technology

The invention discloses a lilium regale WRKY transcription factor gene LrWRKY1. The lilium regale WRKY transcription factor gene LrWRKY1 has a nucleotide sequence shown as SEQ ID NO: 1, and codes a protein with a nucleotide sequence shown as SEQ ID NO: 2. According to the lilium regale Wilson WRKY transcription factor gene LrWRKY1, functional genomics related technical research verifies that the LrWRKY1 gene has the function of increasing the fungus resistance of plants; when the antifungal LrWRKY1 gene is constructed to a plant expression vector and is transferred to tobaccos for overexpression, transgenic tobacco plants have strong in-vitro antifungal activities, and experimental results show that the LrWRKY1 overexpressed transgenic tobaccos have obvious inhibiting effects on the growth of four fungi including botryosphaeria, sclerotinite, botrytis cinerea and fusarium oxysporum.

The invention discloses a lilium regale WRKY transcription factor gene LrWRKY4. The nucleotide sequence of the lilium regale WRKY transcription factor gene LrWRKY4 is described as in the SEQ ID NO:1,and the coded protein of the lilium regale WRKY transcription factor gene LrWRKY4 corresponds to the amino acid sequence described in the SEQ ID NO:2. According to the lilium regale WRKY transcriptionfactor gene LrWRKY4, it is proved that the LrWRKY4 gene has the function of improving the antifungal ability of plants through the technical research related to the functional genomics, the antifungal LrWRKY4 gene is constructed to a plant expression vector and transferred into tobacco for overexpression, the transgenic tobacco has very high antifungal activity, and the experimental result showsthat the tobacco with the LrWRKY4 gene overexpressed is highly resistant to infestation of nigrospora oryzae, fusarium graminearum, fusarium graminearum, botryosphaeria and fusarium solanum.

The invention discloses a Lilium regale Wilson WRKY transcription factor gene LrWRKY11. The Lilium regale Wilson WRKY transcription factor gene LrWRKY11 has a nucleotide sequence shown as SEQ ID NO: 1, and encodes a protein having an amino acid sequences shown as SEQ ID NO: 2. According to the invention, genomic studies have confirmed that the LrWRKY11 gene can improve anti-fungal functions of plants. Being constructed onto a plant expression vector and transferred into tobacco so as to be subjected to overexpression, the anti-fungal LrWRKY11 gene disclosed by the invention is capable of endowing the transgenic tobacco with very strong resistance to fungal pathogens. Experimental results have shown that transgenic tobacco with overexpression of the LrWRKY11 gene has very high level of resistance to infection of Nigrospora oryzae, Fusarium verticillioides, Botryosphaeria dothidea, Fusarium solani and Alternaria panax.

The invention discloses a lilium regale WRKY transcription factor gene LrWRKY3 and a preparation method thereof. A nucleotide sequence of the gene is shown as SEQ ID NO: 1, and a protein with an aminoacid sequence shown as shown in SEQ ID NO: 2; according to the invention, functional genomics related technical researches prove that the LrWRKY3 gene has the function of improving the antifungal performance of plants; the antifungal LrWRKY3 gene disclosed by the invention is constructed on a plant expression vector and is transferred into tobacco for overexpression; the transgenic tobacco plantshave very strong fungus infection resistance, and experimental results show that transgenic tobacco leaves with over-expression of LrWRKY3 have very strong resistance to infection of five pathogenicfungi such as alternaria oryzae, Botryosphaeria dothidea, fusarium graminearum, Fusarium rotunda and fusarium solani.

The invention discloses a lilium regale germin-like protein gene LrGLP2 with antifungal activity. The gene LrGLP2 has the nucleotide sequence as shown in SEQ ID:1 and encodes germin-like protein. A relevant technology of functional genomics proves that the gene LrGLP2 has a function of improving the plant antifungal activity. The antifungal gene LrGLP2 is constructed to a plant expression vector and is transferred into tobacco to perform overexpression; and the transgenic tobacco plant has strong in vitro antifungal activity. The LrGLP2 transgenic tobacco protein has different degrees of inhibition effects on the growth of botryosphaeria dothidea, phomopsis fungi, fusarium oxysporum and botrytis cinerea.

The invention discloses an application of a lilium regale wilson pathogenesis-related protein 10 gene LrPR10-5. A nucleotide sequence of the LrPR10-5 is as shown in SEQIDNO:1, and the pathogenesis-related protein 10 gene is encoded. The research proves that the LrPR10-5 gene has the function of improving the plant antifungal capability by functional genomics-related technologies, the antifungal LrPR10-5 gene is built on a plant expression vector and is switched into tobacco to perform overexpression, the transgenic tobacco plant has strong in-vitro antifungal activity in the result, and the experiment result shows that the transgenic tobacco for overexpression of the LrPR10-5 has an obvious inhibiting effect on growth of a plurality of fungi, such as botrytis cinerea, rhizoctonia solani and sclerotinia sclerotiorum.

The invention discloses a lilium regale inducible promoter PR4 and application thereof, the nucleotide sequence of the PR4 is shown as SEQ ID NO: 1, and the lilium regale inducible promoter PR4 is proved to respond to several plant hormones and biological stress through molecular biology and genetic engineering related technical research; and a fusion expression cassette constructed by the lilium regale promoter PR4 and a beta-glucuronidase gene is transferred into tobacco for expression, and the glucuronidase activity of transgenic tobacco is quantitatively detected through a fluorescence method. Results show that after the transgenic tobacco is treated by methyl jasmonate, salicylic acid, fusarium oxysporum, alternaria alternata and fusarium chlamydosporum, the activity of glucosidase is obviously enhanced; therefore, the lilium regale promoter PR4 is a promoter induced by plant hormones and biological stress factors and can be used for plant disease-resistant gene engineering.

The invention discloses a lilium regale inducible promoter PD1 and application thereof, the nucleotide sequence of the inducible promoter PD1 is shown as SEQ ID NO:1, and the lilium regale inducible promoter PD1 is proved to respond to several plant hormones, biological stress and abiotic stress through molecular biology and genetic engineering related technical research; a fusion gene expression cassette constructed by the lilium regale promoter PD1 and a [beta]-glucuronidase gene is transferred into tobacco for expression, and the glucuronidase activity of transgenic tobacco is quantitatively detected through adoption of a fluorescence method. Results show that after the transgenic tobacco is treated by abscisic acid, salicylic acid, methyl jasmonate, gibberellin, ethephon, fusarium solani, injury stress and sodium chloride, the activity of glucosidase is obviously enhanced; and the inducible promoter PD1 disclosed by the invention has a wide application prospect in genetic engineering for resisting biological or abiotic stress.

The invention discloses an inducible promoter PCHI which is derived from lilium regale. The nucleotide sequence of the inducible promoter PCHI is shown as SEQ ID NO: 1. According to the invention, technical research related to molecular biology and genetic engineering proves that the lilium regale promoter PCHI responds to jasmonic acid methyl ester and biological stress. An expression cassette constructed by the lilium regale promoter PCHI and a beta-glucosidase gene is transferred into tobacco for expression, and the glucosidase activity of the obtained transgenic tobacco is quantitatively detected through a fluorescence method; and results show that after the transgenic tobacco is treated with jasmonic acid methyl ester, fusarium oxysporum, fusarium solani and sporotrichum oryzae treatment, the glucosidase activity of the transgenic tobacco is obviously enhanced. The lilium regale promoter PCHI is induced by jasmonic acid methyl ester and biological stress factors and can be used in disease-resistant gene engineering of plants.

The invention discloses a lilium regale inducible promoter PG1. A nucleotide sequence of the lilium regale inducible promoter PG1 is as shown in SEQ ID NO:1. According to the invention, molecular biology and genetic engineering related technical researches prove that the lilium regale inducible promoter PG1 responds to a few plant hormones, organisms and abiotic stresses; a fusion expression cassette constructed by the lilium regale promoter PG1 and a beta-glucosidase gene is transferred into tobacco to be expressed; the glucosidase activity of transgenic tobacco is quantitatively detected through a fluorescence method; a result shows that after abscisic acid, salicylic acid, fusarium oxysporum, nigrospora oryzae, alternaria compact and damage stress treatment, the glucosidase activity of the transgenic tobacco is obviously enhanced; and the lilium regale promoter PG1 is induced by a few plant hormones, organisms and abiotic stress factors, and has a wide application prospect in biological or abiotic stress resistant genetic engineering.

The invention discloses a lilium regale wilson LrWRKY2 gene and application. A nucleotide sequence of the LrWRKY2 gene is shown in SEQ ID NO.1, and the coded amino acid sequence of the gene is shown in SEQ ID NO.2. According to the gene, an expression carrier with the LrWRKY2 gene is transferred into an arabidopsis thaliana plant, the acquired transgenic arabidopsis thaliana plant shows creep growth, and the original growth form of wild arabidopsis thaliana is thoroughly changed; multiple lotus-throne-shaped basal leaves grow up on the arabidopsis thaliana stems with creep growth, the edges ofthe leaves have a special regular-protruding form, the arabidopsis thaliana is extremely suitable for large-scale cultivation and application in a courtyard and landscaping, and meanwhile the gene provides the theoretical basis and technical support for preparation of a novel ground cover plant and has potential application prospects.

The invention discloses a lilium regale wilson LrPAL-1 gene and application thereof. The nucleotide sequence of the LrPAL-1 gene is as shown in SEQ ID NO.1 and the coded amino acid sequence of the LrPAL-1 gene is as shown in SEQ ID NO.2. The overexpression vector containing the LrPAL-1 gene is transferred into tobacco, the lignification degree of an obtained transgenic tobacco plant is increased,the lodging resistance of the plant is improved, and particularly, the upright state of fresh cut lily flowers can be maintained. The transgenic plant has an obvious inhibition effect on pathogenic bacteria botrytis cinerea and alternaria alternata. Meanwhile, the functional genomics researches prove that the lilium regale wilson LrPAL-1 gene has an important biological function of changing the morphology of plant leaves. The invention provides an important target for molecular genetic improvement of lilium plants; the lilium regale wilson LrPAL-1 gene has an important value and significance for cultivation and production of new varieties of plants with ornamental value and landscape ecological value; and a technical support is provided for cultivation of new lilium germplasm with dual values of lodging resistance and pathogenic bacteria resistance.

The invention discloses a glutathione S-transferase gene LrGSTU5, wherein the nucleotide sequence of the gene is shown as SEQ ID NO: 1; and the gene encodes the glutathione S-transferase. According to the invention, related technology of functional genomics is adopted to prove that the lilium regale LrGSTU5 gene has the function of improving the antifungal ability of plants; the lilium regale glutathione S-transferase gene LrGSTU5 provided by the invention is built on plant expression carriers and then transferred into tobacco for overexpression; the transgenic tobacco has a high in-vitro antifungal activity; and the transgenic tobacco protein expressing LrGSTU5 has an obvious inhibition effect on the growth of various pathogenic fungi such as botryosphaeria dothidea, fusarium oxysporum, alternaria sp and the like.

The invention discloses a lilium regale Lr4CL-1 gene and application. A nucleotide sequence of the Lr4CL-1 gene is as shown in SEQ ID NO.1, an amino acid sequence coded by the Lr4CL-1 gene is as shownin SEQ ID NO.2, an overexpression vector containing the Lr4CL-1 gene is transferred into tobacco, an obtained transgenic tobacco plant has obvious inhibiting effects on pathogenic bacteria of botrytis cinerea, alternaria alternata, anthracnose and the like, and meanwhile, the fact that leaves of the transgenic plant is more dark-green and slightly droop is found. According to the lilium regale Lr4CL-1 gene and the application, through studies of functional genomics, the fact that the Lr4CL-1 gene has double functions of improving fungus resistance of plants and changing the leaf characters isproven, and theory and technique support is provided for using the gene for conducting plant molecular heredity improvement in future, so that the lilium regale Lr4CL-1 gene has potential applicationvalues.

The invention discloses a susceptible fungal gene LrWRKY-S1 of lily and application thereof. A nucleotide sequence of the LrWRKY-S1 gene is shown by SEQ ID NO.1 and an amino acid sequence coded thereby is shown by SEQ ID NO.2. Biological functions of the protein are studied by a bacteriostasis experiment. Experimental results show the protein makes plants more susceptible to pathogenic fungi suchas Botrytis cinerea, fusarium oxysporum and Colletotrichum orbiculare. As found as well, the LrWRKY-S1 gene has a low expression level in lilium regale with high resistance, and a homologous gene thereof has high expression levels in lilium davidii, lilium longifolorum and lilium lancifolium with important economic values. The gene and the application thereof provide an efficient susceptible genefor genetic engineering reconstruction, lays a foundation for further cultivation of fungus-resistant / susceptible lily or new materials or new species of other plants, can provide theoretical and technical support for later application of the gene in plant genetic improvement, broadens ideas for prevention and control of plant diseases and has very extensive application values.

The nucleotide sequence of the lilium regale chitinase gene LrCHI2 is shown as SEQ ID NO: 1, and the lilium regale chitinase gene LrCHI2 encodes a protein with an amino acid sequence shown as SEQ ID NO: 2. Functional genomics related technical researches prove that the LrCHI2 gene has the function of improving the resistance of plants to pathogenic fungi. The antifungal gene LrCHI2 disclosed by the invention is constructed on a plant expression vector and transferred into tobacco for overexpression, and the transgenic tobacco for overexpression of LrCHI2 has relatively strong capability of resisting infection of alternaria oryzae, aspergillus oryzae and fusarium oxysporum.

The invention discloses an anti-disease gene (LrGSTL1) of lilium regale and an application thereof. The nucleotide sequence of the gene (LrGSTL1) is shown as SEQIDNO:1 to encode Lambda glutathione S-transferase. Functional genomics related technical research shows that the gene (LrGSTL1) of lilium regale has the function of improving the antifungal ability of the plant. The glutathione S-transferase gene (LrGSTL1) of lilium regale disclosed by the invention is constructed to a plant expression vector and transformed to tobacco to be over-expressed. The transgenetic tobacco has strong antifungal activity, and the transgenetic tobacco sheet which expresses (LrGSTL1) has remarkable resistance to infection by fusarium oxysporum and botrytis cinerea.

The invention provides a minjiang lily spice composite as a new-type lily spice composite which is characterized in that: at least one of jasmal, geranyl acetate, terpene-4-ethanol, dimethoxy methyl benzol, methyl anthranilate and anisyl acetate is added in a base composite based on the lily spice, further comprising at least one spice regulator of triethyl citrate, diisobutyl adipate, tetrahydrophthalic anhydride abietic acid methyl ester and Dihydrogenated abietic acid methyl ester.

The invention relates to an application of a lilium regale germin protein gene LrGLP1. The nucleotide sequence of the LrGLP1gene is shown as SEQ ID NO:1, a germin-like protein is encoded, and according to the invention, functional genomics related technical researches prove that the LrGLP1gene has a function of improving the fungal infection resistance of plants. An antifungal LrGLP1 gene in the invention is built into a plant expression vector, and transferred into a tobacco to be subjected to excessive expression, so that a transgenic tobacco plant has an extremely strong in-vitro antifungal activity. LrGLP1overexpressed transgenic tobaccos have an obvious inhibitory effect on the growth of alternaria alternate, sclerotinia sclerotiorum and catenuliform gibberella.

The invention discloses a lilium regale wilson LrCCoAOMT gene and application thereof. The nucleotide sequence of the LrCCoAOMT gene is shown in SEQ ID NO.1, and the amino acid sequence coded by the gene is shown in SEQ ID NO.2. An overexpression vector containing the LrCCoAOMT gene is transferred into arabidopsis thaliana to obtain genetically modified arabidopsis thaliana, compared with a wild plant, the stem of the genetically modified arabidopsis thaliana is thicker and stronger, rosette leaves are larger, and the lignin content is obviously increased. Through functional genomics research,it is further proved that the LrCCoAOMT gene can improve the lodging resistance of the plant and enhance the resistance of plants to pathogenic fungi, especially botryis cinerea and alternaria alternata, the LrCCoAOMT gene plays an important role in breeding work of the plants, and the yield and the quality of crops are easily improved; the LrCCoAOMT gene has an important theoretical and practical significance in improving the plant quality and other key agronomic traits of the crops, and further provides a new means for regulating the resistance by using genetic engineering.

The invention discloses a gene (LrGSTU3) of lilium regale with antifungal activity and an application thereof. The nucleotide sequence of the gene (LrGSTU3) is shown as SEQIDNO:1 to encode tau glutathione S-transferase. Functional genomics related technical research shows that the gene (LrGSTU3) of lilium regale has the function of improving the antifungal ability of the plant. The gene (LrGSTU3) of lilium regale disclosed by the invention is constructed to a plant expression vector and transformed to tobacco to be over-expressed, so that the transgenetic tobacco strain is strong in vitro antifungal activity, and the transgenetic tobacco sheet which over-expresses (LrGSTU3) has a remarkable inhibiting effect on growth of fusarium oxysporum and botrytis cinerea.

The invention discloses a Lilium regale Wilson Dirigent similar protein gene LrDIR1. The nucleotide sequence of the Lilium regale Wilson Dirigent similar protein gene LrDIR1 is as shown in SEQ ID NO: 1, and a protein with an amino acid sequence as shown in SEQ ID NO: 2 is encoded. Through the related technology research of the functional genomics, the LrDef1 gene is proved to have a function of improving the antifungal ability of plants. The antifungal LrDIR1 gene disclosed by the invention is constructed on a plant expression vector and transferred into tobacco for overexpression, a transgenic tobacco plant has a very strong antifungal infection ability, and experimental results show that the transgenic tobacco subjected to LrDIR1 overexpression has resistance to infection of fusarium oxysporum and Phoma herbarum.

The invention discloses a lilium regale LrWRKY25 gene and application. The nucleotide sequence of the lilium regale LrWRKY25 gene is shown as SEQ ID NO.1, and the coded amino acid sequence is shown asSEQ ID NO.2. An expression vector containing the LrWRKY25 gene is transferred into Arabidopsis plants, the obtained transgenic Arabidopsis plants show creep growth, and the original growth form of wild-type Arabidopsis is completely changed; and moreover, multiple rosette basal leaves grow from Arabidopsis stems in creep growth, and the leaf edge has a special form of regular projections. The lilium regale LrWRKY25 gene is very suitable for large-area cultivation and application in gardens and landscaping. Meanwhile, the lilium regale LrWRKY25 gene disclosed by the invention provides a theoretical basis and a technical support for preparing novel ground cover plants, and has potential application prospects.

The invention discloses a scale-cutting method for lilium regale Wilson. The scale-cutting method comprises the following steps that S1, scales from the lilium regale Wilson are selected, wherein bulbs from the lilium regale Wilson of a good variety are selected, the seed bulbs are soaked with 0.1% carbendazim for 30 minutes, two to three layers of scales which are aged and died or rotten on the outer side of each seed bulb from the lilium regale Wilson are removed, the remaining scales are stripped off, and the seed bulbs are disinfected with 0.5% potassium permanganate for 8 minutes, then put into a shade place and naturally aired for 24 hours; S2, a culture pot and a cutting medium are prepared; S3, the scales from the lilium regale Wilson are treated with a hormone, wherein the scalesfrom the lilium regale Wilson are subjected to quick dipping by using 50 mg / L of NAA, and then soaked with 30 mg / L of IBA for 4 hours; S4, cutting is conducted in a buried cutting way. The scale-cutting method for the lilium regale Wilson is low in breeding cost, convenient to operate, high in breeding coefficient and beneficial for large-scale breeding, and the production period can be shortenedwhile excellent characters are maintained.

The invention discloses a lilium regale plant dwarfing method. The method comprises the following steps that S1, lilium regale seedballs of the same size are selected, soaked in 500 times of 50% carbendazim for 30 minutes, taken out, dried and then planted; S2, When lilium regale germinates and grows to 14-16 centimeters, the lilium regale is treated with plant growth regulators separately; the specific implementation method comprises the steps that one-time spraying is carried out on the lilium regale plants with 3200 mg / L CCC, 800 mg / L PP333 and 2000 mg / L B9 respectively; or two-time spraying is carried out on the lilium regale plants with 800 mg / L CCC, 800 mg / L PP333 and 8000 mg / L B9 respectively, wherein the time interval of the two-time spraying is 15 days. The lilium regale plant dwarfing method achieves lilium regale plant dwarfing by using the plant growth regulator, ultraviolet lamp radiation treatment and other methods, increases the ornamental value of the lilium regale plants as potted flowers, and facilitates the commercial development of the lilium regale potted flowers.