The invention discloses a method suitable for evaluating the top-free flooding resistance of a tropical
water lily stock
plant and a fetal
seedling, and relates to the field of adversity stress, and the
water lily stock
plant or the fetal
seedling is subjected to top-free flooding treatment with different time lengths. Determining the damage condition by taking the leaf length, the
leaf width, the leaf ear length, the leaf area, the
stem length, the stem
diameter, the
chlorophyll content and the
leaf cell structure of the normally growing and waterlogged
plant as evaluation indexes, and calculating the leaf rot index and the flood tolerance index of the to-be-detected
water lily according to the flood damage leaf rot area to realize evaluation. According to the method, the influence of waterlogging on the external
phenotype and internal structure of the water lily is considered, the difference of waterlogging treatment on plant ages is considered, the waterlogging resistance is determined through
phenotype and
cell structure changes, the waterlogging resistance level of the tropical fetal water lily is determined, and the method has the remarkable advantages of being short in
evaluation period, visual, reliable and the like. The method is beneficial for promoting innovation of water lily flooding-resistant
germplasm and breeding of a flooding-resistant fetal water lily variety, and a new method is provided for research of a water lily flooding-resistant mechanism.