760 results about "Bacillus pumilus" patented technology

The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.

The invention discloses a denitrifying strain with nitrifying function, a strain-containing water body improver of multiple active microorganisms and a preparation method of the water body improver, belonging to the technical fields of aquaculture and water body improvement. The strain disclosed by the invention is conserved in the China center for type culture collection (CCTCC), the CCTCC NO isM2011443; and the water body improver of the active microorganisms which is based on the strain comprises multiple strains such as Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Bacillus cereus, Bacillus megaterium, Rhodopeudomonas palustris, Arthrobacter globiformis, Rhodotorula rubra and Lactobacillus plantarum. The strain is used to perform liquid fermentation and obtain fermentation liquor of which number of live bacteria is 0.5-3*10<10>cfu/mL, bacterial powder of which the number of live bacteria is 0.2-2*10<12>cfu/mL is further prepared; and the bacterial powder is used along with a diluent to prepare a liquid or powdery water body improver, wherein the effective live bacteria of the water body improver is 0.1-3*10<10>cfu/mL(or cfu/g). The water body improver has the function of reducing the contents of ammonia nitrogen, nitrites and nitrates in the culture water body; and the water body improver is suitable for different culture water bodies and has the function of improving the water quality.

The invention relates to an environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture. An environment-friendly composite microbial water purifying agent comprises the following components in percentage by weight: 20 to 40 percent of bacillus subtilis, 10 to 20 percent of bacillus licheniformis, 10 to 15 percent of bacillus megaterium, 10 to 15 percent of brevibacillus laterosporus, 5 to 10 percent of bacillus pumilus, 5 to 20 percent of bacillus coagulans and 2 percent of biological activating agent. The high-concentration composite microbial preparation is prepared by screening eight strains of probiotics by simulating the physiological functions of normal microbial flora in an aquaculture environment and through scientific compatibility, so that division and cooperation amount the bacterial strains can be realized, a biological enhancement technology can be combined with an indigenous flora activating technology, complex problems in a culture water body can be solved completely, the using amount is small, the cost is low, and the competitive advantage is remarkable.

The invention belongs to the technical field of feed micro-ecology and relates to a composite micro-ecological preparation as well as a premixed material and an application of the preparation in a feed additive. The preparation contains Lactobacillus acidophilus powder and any two, three or four bacterium powders selected from Bacillus licheniformis, Bcillus pumilus, enteroccus and Bcillus subtilis; the preparation has high live bacteria content and low moisture content, has stress tolerance properties such as gastric acid resistance, cholate resistance, high temperature resistance, common antibiotics tolerance and the like and has the probiotic functions of producing acid and enzyme, inhibiting pathogenic bacteria and the like. By using the composite micro-ecological preparation of the invention, the utilization rate of the feed can be improved, the yields of meat, eggs, milk and the like are increased, the growth of animals is promoted, the immunity and disease-resistant capability of an animal organism are improved, and partial preparation can be used for replacing antibiotics, thereby changing the quality of an animal product.

An aqueous composition is disclosed, comprising at least one strain of Class 1 Bacillus bacterial spores, selected from Bacillus Cirulans, Bacillus Megaterium, Bacillus Lichenforms, Bacillus Pumilus, Bacillus Sphaericus, Bacillus Subtilis sub species 10144 and Bacillus Subtilis sub species 8646, a terpene and one or more surfactants. The terpene is preferably d-limonene. A method for cleaning surfaces is also disclosed, which method comprises deploying an aqueous composition comprising at least one strain of Class 1 Bacillus bacterial spores, selected from Bacillus Cirulans, Bacillus Megaterium, Bacillus Lichenforms, Bacillus Pumilus, Bacillus Sphaericus, Bacillus Subtilis sub species 10144 and Bacillus Subtilis sub species 8646, a terpene and one or more surfactants to a surface.

The invention discloses a microbial inoculum for bioremediating a coastline polluted by oil spilling and a preparation method thereof. The microbial inoculum for bioremediating the coastline polluted by oil spilling is made of mixed fermentation liquor and a fixed carrier as raw materials, wherein the mixed fermentation liquor comprises bacillus pumilus CGMCCnO.3384 fermentation liquor, Venice acinetobacter CGMCCnO.3385 fermentation liquor and/or Doxa pseudomonad CGMCCnO.3386 fermentation liquor and/or Ralstonia pickettii CGMCCnO.3387 fermentation liquor. By using the synergic action of different strains on the degradation of various petroleum hydrocarbons, the microbial inoculum improves the degradation speed of petroleum hydrocarbon pollutants and thoroughly degrades various petroleum hydrocarbon pollutants; in addition, a flora formed by a plurality of strains of bacteria has more stable structure and can better adapt to various petroleum-polluted environments. The microbial inoculum rapidly grows on the polluted areas, forms competitive advantages and remarkably increases the quantity of hydrocarbon degraded microbes in the polluted areas, thereby improving the biodegradation rate of crude oil.

The invention discloses bacillus pumilus and application thereof, which belong to the technical field of microbes. The produced strain of the invention is named Bacillus pumilus Van35 according to classification, and the preservation number of the strain is CGMCC No.3411. When the strain preparation is spayed, the soluble total sugar content and the reducing sugar content of tobaccos can be increased, while protein, total nitrogen and nicotine content are reduced. Therefore, specific values of the ratio of dextrose to nitrogen, the ratio of total sugar to betaine and the ratio of total nitrogen to total alkaloid tend to rise, the quality of chemicals is high and the quality of the smoke panel test is improved.

The invention relates to a host cell and a method for the host cell applied in efficient secretion expression of a recombinant protein, which belongs to the filed of microbiological engineering and fermentation engineering. The invention provides a bacillus licheniformis host cell CBB3008 which is preserved in the China Center for Type Culture Collection with the preserving number of CCTCC NO: M 208236. The invention transforms an expression plasmid of an industrial enzyme which is obtained by a gene cloning technology into the host cell to synthesize an industrial enzyme preparation in the host cell efficiently, and then secretes the synthesized enzyme protein into a culture medium efficiently through a protein secretion system of the host cell, thus the invention can guide efficient secretion production of the industrial enzyme preparation. Therefore, the invention is helpful to reduce the fermentative production cost of the industrial enzyme preparation, simplify the fermentative production process and reduce the environmental pressure of the fermentation industry. A method for host cell screening and genetic improvement of the invention can also be used for other types of host cells, in particular for the breeding of the host cells of bacillus subtilis, bacillus megaterium, bacillus pumilus, bacillus starch solution and the like.

The invention discloses a composite microbial seed dressing agent and a preparation method and an application of the composite microbial seed dressing agent. The composite microbial seed dressing agent is compounded by sinorhizobium fredii, bacillus coagulans, bacillus licheniformis, bacillus pumilus, bacillus amyloliquefaciens and turf. The composite microbial seed dressing agent has the effects of fixing nitrogen, solubilizing phosphorus, dissolving potassium, dissolving cellulose and starch, producing plant growth hormone, resisting diseases and the like, also has the function of stress resistance, and is good in mutual compatibility, easy to control over culture conditions and the like; the composite microbial seed dressing agent can be evenly stirred and sowed together with seeds; and the compound fertilizer is used as a base fertilizer to reduce use of a compound fertilizer; therefore, the composite microbial seed dressing agent has relatively high market value.

The invention discloses bacillus pumilus GBSW19 which is preserved in CGMCC (China General Microbiological Culture Collection Center), the preservation date is 9th September, 2013, and the preservation number is CGMCC No. 8140. The bacillus pumilus GBSW19 can be used for crop straw degradation and simultaneously has functions of inhibiting plant pathogen such as rhizoctonia solani , Xanthamonas oryzae pv.oryzae and the like, so that the growth of rice is promoted, and the bacillus pumilus GBSW19 has a good application prospect.

The invention relates to a bacillus pumilus (Bacillus pumilus) L-9 laccase gene cotA as well as recombinant expression and application thereof, belonging to the field of bioengineering. The cotA is separated from the bacillus pumilus (Bacillus pumilus) L-9, an expression vector pColdII-cotA is constructed, and an escherichia coli expression strain BL21(DE3) is transformed to realize high-efficiency expression. The obtained recombinase laccase has the advantages of high alkaline environment resistance and high high-temperature resistance, has a good effect of discoloring azo dyes and anthraquinone dyes and has high application potential.

The invention relates to Bacillus pumilus NMCC 46, which is preserved in CGMCC with preservation date as November 2, 2009, and preservation number as CGMCC No.3378. The Bacillus pumilus NMCC 46 CGMCC No.3378 in the invention is a biological control bacillus separated from grass root surrounding soil near Namco Lake in Tibet, which can grow at 10 DEG C, is favorable for exerting growth promotion and biological control effects, can restrain soybean root pythium myriotylum and saccharomycetes, can boost growth of Arabidopsis and soybean, has a prevention and control effects on soybean root pythium myriotylum as high as 85%, can increase yield of soybean to 24%, and has good application prospect.

The invention discloses a complex endophytic bacterial inoculant and an application thereof in phytoremediation of heavy metal contaminated soil. The complex inoculant is composed of acinetobactercalcoaceticus, pseudomonas fluorescens, bacillusmegaterium, stenotrophomonasmaltophilia, bacillus pumilus and pseudomonas putida. The complex inoculant disclosed by the invention, when applied to heavy metal pollution remediation, integrates advantages of various endophytic bacteria and overcomes disadvantages of phytoremediation; and the complex inoculant can promote the growth of sedum alfredii hance, enhance the absorbing capacity of the sedum alfredii hance to heavy metals and improve the efficiency of phytoremediation.

The invention relates to the technical field of microbe application, specifically relates to a microbial agent and application thereof to saline-alkali land soil improvement. The microbial agent is prepared by compounding bacillus amyloliquefaciens Bn-21, Bacillus subtilis Bn-3,Bacillus pumilus Bn-19, Rhodopseudomonas palustris Bn-2 and lactobacillus plantarum Bn-23, the compounded multiple microbes has different functions, each microbe carries out different microbe metabolic activity, every two bacterial strains have no antagonism, the function of each microbe is relatively stable, can play a different role in a soil conditioner, increase microbe diversity in soil, enhance activity of the microbe in soil, strengthen the effects of the soil conditioner for improving soil physical and chemical properties and improving the soil fertility.

The invention relates to bacillus pumilus NMCN1, which is collected at China General Microbiological Culture Collection Centre (CGMCC) on November 2, 2009 with a collection number of CGMCC No. 3377. The bacillus pumilus NMCN1CGMCC No. 3377 is biocontrol bacillus which is separated from lakeside soil of Tibet Namsto Lake; and the biocontrol bacillus can grow at the temperature of 10 DEG C, is favorable for playing a role in growth promotion and biological control, can suppress pathogenic bacteria and yeast of tomato gray mold, promote the growth of arabidopsis thaliana and tomato, particularly has a prevention and control effect of 75 percent on the tomato gray mold and can also increase the output of the tomato by 12 percent.

The invention discloses a bacillus pumilus JK-SX001 which is classified and named bacillus pumilus and collected in a China center for type culture collection (CCTCC) and has the collection number ofM 209026 on February 18th, 2009. The invention also discloses applications of the bacillus pumilus in preventing and treating dothiorella gregaria and promoting the growth of a poplar. The invention also provides a bactericide for preventing and treating the dothiorella gregaria, which is obtained by vaccinating the JK-SX001 in a culture medium to ferment and culture. The JK-SX001 is a plant endophyte, has obvious inhibiting effect on dothiorella gregaria, simple culture condition, easy storage and favorable development and application prospect and is easy for industrialized production.

The invention discloses a solid microbial bacterium agent for petroleum degradation. The agent is prepared by mixing of the following components by weight: 85-90 parts of a microbial bacterial fertilizer, and 10-15 parts of a mixed fermentation solution of Micrococcus luteus, Bacillus cereus and Bacillus pumilus. In addition, the invention also provides a preparation method of the solid microbialbacterium agent. The solid microbial bacterium agent provided in the invention can introduce beneficial microorganisms into the soil, optimize the soil microbial system, enhance physical properties of the soil, enhance soil biological activity, and restore hardened and degraded soil, thus being an eco-environment-friendly green bacterium agent. Application of the solid microbial bacterium agent provided in the invention in the soil can promote crop growth, degrade and transform petroleum pollutants in the soil, and also can enhance the stress resistance of crops, as well as improve crop yield.

The invention discloses a fermentation liquor composition used for preparing microbial organic fertilizer, which comprises the components based on the weight percentage: 60-70% of bacillus subtilis fermentation liquor, 15-20 % of bacillus pumilus fermentation liquor and 15-20 % of pseudomonas fluorescens fermentation liquor. The invention also provides a preparation method of the fermentation liquor composition, which comprises: bacillus subtilis, bacillus pumilus and the pseudomonas fluorescens are respectively fermented separately, and then the obtained fermentation liquors are mixed. The strain of the fermentation liquor composition has optimal combination, so as to realize versatility. The selected strain not only has effect of growth promotion, but also has antagonism effect for multiple plant soil-borne diseases. The bacillus subtilis and the pseudomonas fluorescens in the product are plant rhizosphere growth-promoting bacteria which can stimulate and induce the systemic resistance of the crops, improve the self immunity and enhance disease resistance.