117 results about "Bacillus pumilus" patented technology

The invention relates to a complex microbial inoculant for preventing and treating citrus huanglongbing as well as a preparation method and application of the complex microbial inoculant. The compound bacterial agent for preventing and treating the citrus huanglongbing is prepared from the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. The compound bacterial agent for preventing and treating the citrus huanglongbing comprises the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. After the complex microbial inoculant is added, the content of callose in infected leaves can be effectively reduced, the self-resistance mechanism of plants is induced, the disease resistance is improved, and the complex microbial inoculant has an obvious inhibiting effect and a growth promoting effect on Candidatus Liberibacter asiaticum.

The invention relates to bacillus pumilus and application thereof. The bacillus pumilus DX-fK is obtained through a chemical mutagenesis method, the bacillus pumilus DX-fK can efficiently degrade straw, the bacillus pumilus DX-fK is used as a substrate to produce feed alternative protein, and therefore the bacillus pumilus DX-fK can be applied industrially and has good industrialization prospects.

The invention relates to the technical field of agricultural planting, in particular to a method for preventing and treating watermelon fusarium wilt by using a green bactericide, which comprises the following steps: preparing the green bactericide which comprises the following components in percentage by mass: 18-25% of composite plant source active component consisting of water lettuce extract, enteromorpha polysaccharide and field horsetail flavone; 20%-30% of an antagonistic bacterium compound preparation, wherein the antagonistic bacterium compound preparation comprises bacillus pumilus and trichoderma; 38%-45% of a nano-carrier, wherein a chitosan-sodium alginate compound is used as an embedding material; and the balance of auxiliary components. Applying the green bactericide to watermelon plants; through component optimization and a synergistic effect, compared with the prior art, the composition has the remarkable advantages that the safety is high, all the components are from natural plants or microorganisms, no chemical pesticide residue exists, the composition is healthy to human bodies and environmentally friendly, and the problems of drug resistance and soil pollution caused by chemical agents can be avoided.

The invention discloses a salt-tolerant brevibacillus for promoting growth and preventing and controlling plant diseases and application of the salt-tolerant brevibacillus. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025, and the preservation number of the brevibacillus halotolerans T101 is GDMCC No: 66731. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025. The strain T101 disclosed by the invention can effectively antagonize plant pathogenic fungi peronophythora litchii and plant pathogenic bacteria such as pseudomonas syringae, xanthomonas campestris, citrus canker pathogen and ralstonia solanacearum, can generate siderophores and plant growth hormone IAA, and can remarkably promote the growth of tomato root systems in a pot experiment. Therefore, the strain T101 can be used for preparing microbial preparation products for preventing and treating plant diseases and promoting plant root growth, and has important application potential in the aspects of microbial agents, biological fertilizers and the like for promoting plant growth and development.

The invention relates to the technical field of agricultural biology, and discloses a microbial agent for preventing and treating fructus aurantii scab and a preparation method of the microbial agent. The microbial agent comprises the following raw materials in percentage by weight: 10-13% of paenibacillus mucilaginosus; 15%-20% of trichoderma sp. 7%-10% of paecilomyces lilacinus; 5%-8% of bacillus pumilus; 7%-9% of mycorrhizal fungi; 5%-8% of streptomyces; 5%-9% of a coptis chinensis extract; 4%-7% of a sophora flavescens extract; 2%-4% of realgar; 1%-3% of a rheum officinale extract; 10%-13% of soybean meal; 5%-8% of a water-soluble fertilizer; 7%-9% of potassium fulvate; 1%-3% of chitosan; 0.5%-1% of trace elements; 3%-8% of humic acid; the raw materials are subjected to microbial flora amplification culture, plant antibacterial component light-shielding mixing and low-temperature mixing to effectively protect the microbial activity of the microbial agent, and step-by-step operation can avoid damage of high temperature and mechanical shearing to microorganisms, so that the microbial activity of the microbial agent is effectively protected. And the loss of microbial active ingredients is reduced by vacuum drying and low-temperature granulation processes.

The invention relates to the technical field of resource utilization of rotten vegetable waste, in particular to a microbial agent and application thereof in the field of rapid rotting of rotten vegetable waste. The microbial agent comprises bacillus pumilus and bacillus subtilis, bacillus subtilis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33122; the bacillus pumilus is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 33121. The invention also provides an application of the microbial agent in the field of rapid rotting of rotten vegetable leaves. The treatment method for rapid rotting of the rotten vegetable leaves is widened, the types of microorganisms capable of being used for rapid rotting of the rotten vegetable leaves are enriched, the rotting rate of the rotten vegetable leaves can be effectively increased when the microbial agent is used for rapid rotting of the rotten vegetable leaves, and meanwhile the yield of next-stubble crops can be effectively increased.

The present invention provides an essence containing chlorella extract and a preparation method thereof. The chlorella extract process comprises the following steps: raw material pulverization; enzymatic hydrolysis with cellulase and trypsin; primary fermentation using a mixture of Bacillus natto and Bacillus pumilus; addition of bamboo leaf extract, corn steep liquor, and sucrose, followed by inoculation with Rhodotorula glutinosus for secondary fermentation to obtain the chlorella extract. The mixed fermentation of multiple strains exhibits a synergistic effect, resulting in greater enrichment of natural products during the fermentation process compared to single-strain fermentation. Furthermore, the present invention provides an essence containing chlorella extract, glycerin, sodium hyaluronate, honey, and water, which exhibits significant moisturizing, antioxidant, and soothing effects.

The invention discloses a strain NJSH56 for promoting plant growth and application thereof, the strain NJSH56 is identified as bacillus pumilus, the strain NJSH56 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation time is November 14, 2024, and the strain preservation number is CGMCC NO.32614. The strain NJSH56 can promote plant growth. The dendrobium officinale endophytic growth-promoting strain NJSH56 for promoting plant growth is obtained through separation and screening, growth of dendrobium officinale tissue culture seedlings can be effectively promoted, and the fresh weight, the rooting number and the leaf number are increased; greenhouse tests show that the number of new buds of dendrobium officinale plants in a strain NJSH56 treatment group reaches 4.67 and is obviously higher than that of a control group; meanwhile, the NJSH56 is used for treating the non-host plant Chinese cabbage, so that the growth of the overground part of the Chinese cabbage can be remarkably promoted, and the biomass accumulation of the Chinese cabbage is improved.

When fed to animals, Bacillus licheniformis strain O42AH3 (deposit accession number DSM 32559), Bacillus subtilis strain O52YJ6 (deposit accession number DSM 32560), Bacillus amyloliquefaciens strain O52YYT (deposit accession number DSM 32561) and / or Bacillus pumilis O72NR7 (deposit accession number DSM 32563) are useful for preventing and / or treating Lawsonia infections and for improving myriad performance parameters, including, but not limited to, body weight gain, average daily gain and feed conversion rates.

The invention relates to the technical field of lubricating oil, and discloses degradable emulsified antirust lubricating oil and a preparation method thereof.The lubricating oil is prepared from, by weight, 10-20% of cobalt salt antirust component, 8-20% of emulsifier, 5-8% of fungi, 10-20% of lubricant, 0-1% of coupling agent, 0-0.2% of corrosion inhibitor, 3-6% of stabilizer and the balance base oil; the cobalt salt anti-rust component is a metal salt anti-rust component taking cobalt salt as a main component; the fungus is any one or a mixture of several of bacillus pumilus, bacillus subtilis and bacillus cereus; the preparation method comprises the following steps: adding the cobalt salt antirust component, the co-emulsifier, the fungi, the lubricant, the coupling agent, the corrosion inhibitor, the stabilizer and the base oil into a reaction kettle, heating to 45-50 DEG C, and uniformly stirring; and at the temperature of 45-50 DEG C, adding the main emulsifier while stirring until the system is uniform and transparent, thereby obtaining the anti-rust lubricating oil. The problems that existing antirust lubricating oil is large in smell, and water for cleaning metal workpieces pollutes the environment are solved.

The invention belongs to the technical field of microbial agents, and particularly relates to a microbial agent containing bacillus velezensis and application of the microbial agent to astragalus membranaceus. The invention discloses a microbial agent for astragalus membranaceus. The microbial agent is prepared by mixing bacillus velezensis powder, bacillus subtilis powder and bacillus pumilus powder according to the mass ratio of 1: (1-10): (1-5), the bacterium content of the bacillus velezensis powder, the bacillus subtilis powder and the bacillus pumilus powder is 0.2-0.5 billion cfu / g. The bacillus velezensis, the bacillus subtilis and the bacillus pumilus are fermented respectively, fermentation liquor is taken to be prepared into powder, and the powder is mixed according to a certain mass ratio to form the compound microbial agent, so that the compound microbial agent can improve the prevention and treatment effect on the radix astragali root knot nematode disease, is green and environment-friendly, can effectively overcome the defects of chemical agents, and has a good application prospect. Support is provided for biological prevention and control of the radix astragali root knot nematode disease, and meanwhile healthy growth of radix astragali plants can be guaranteed.

The invention provides a compound feed additive for relieving stress response of marine fishes, which is prepared by the following steps: mixing raw materials such as hermetia illucens protein and the like to prepare a premix, and embedding the premix by using a lac-glycyrrhizic acid system to prepare lac microcapsules; then modifying the fucoidin by adopting glycyrrhizin and gamma-linolenic acid, embedding rhodosporidium palustris and bacillus pumilus by using the fucoidin to prepare compound bacterium nano-particles, and finally mixing the lac microcapsules and the compound bacterium nano-particles in proportion. According to the invention, excessive secretion of cortisol of marine fish can be effectively inhibited, hermetia illucens protein provides rich nutrition and reduces muscle decomposition, and compound microorganisms are beneficial to maintaining intestinal microecological balance and improving the utilization rate of nutrition. The prepared compound feed additive can be directly splashed into a culture or transportation water body or added into a feed, so that the stress reaction of the marine fish is effectively relieved, the survival rate of the marine fish is increased, and powerful support is provided for culture of the marine fish.

The present disclosure relates generally to compositions comprising Bacillus pumilus having a positive impact on animal performance parameters, the use of Bacillus pumilus for preventing, reducing or controlling bacterial colonization or infection, a method of improving one or more animal performance parameters, and a kit. In particular, the present disclosure relates to Bacillus pumilus DSM 34300 and DSM 34299.

The invention discloses a zinc-selenium-rich sour soup fermentation strain and a preparation method thereof, and belongs to the technical field of microbial fermentation. The composite strain is composed of lactobacillus plantarum MJ-ZX01, saccharomyces cerevisiae MJ-ZX02 and bacillus pumilus MJ-ZX03 according to the weight ratio of 5: 3: 2, all of which are obtained by screening from the zinc-selenium environment of Majiang River in Guizhou and have efficient zinc-selenium enrichment and transformation capacity. The preparation method adopts a stepped fermentation process and comprises the following steps: carrying out anaerobic fermentation at 37 DEG C until the pH value is 4.2 in 0-24 hours under the leading of MJ-ZX01; in 24-72 hours, MJ-ZX02 and MJ-ZX03 cooperate with each other, anaerobic fermentation is performed at 30 DEG C until the pH value is 3.8, and the initial Zn content is controlled to be 50 mg / L, the initial Se content is controlled to be 10 mg / L, and the pressure of the fermentation tank is controlled to be-0.05 MPa. According to the technology, the zinc retention rate is greater than or equal to 85%, the selenium retention rate is greater than or equal to 80%, the flavor substance content is doubled, the fermentation period is shortened to 5 days, the technology is suitable for industrial production, and the nutritional value and market competitiveness of the sour soup can be remarkably improved.

According to the agricultural liquid fertilizer for tomato planting, bacillus licheniformis, bacillus amyloliquefaciens, bacillus mucilaginosus and bacillus pumilus are subjected to liquid fermentation and then mixed to prepare the novel agricultural liquid fertilizer for tomato planting. According to the present invention, the tomato growth promotion effect is significant, the prevention and control effects on tomato early blight, tomato black spot pathogen, tomato fusarium wilt, tomato gray mold and tomato anthracnose are significant, the tomato survival rate can be improved, the promotion effect is provided, the fruit quality is improved, the important significance is provided for tomato planting, and the economic benefit is significant. The liquid fertilizer can change the soil flora structure, increases the soil fertility and permeability, and plays an important role in soil improvement.

The invention belongs to the technical field of microorganisms and application, and particularly relates to bacillus pumilus K40, a culture method of the bacillus pumilus K40, a microbial agent containing the bacillus pumilus K40 and application of the bacillus pumilus K40. The invention provides a bacillus pumilus K40 with a preservation number of CCTCC NO: M 2025655, the bacillus pumilus K40 is preserved in China Center for Type Culture Collection on March 31, 2025, and the preservation address is No. 299 on eight road in Wuchang District, Wuhan City, Hubei Province. The strain K40 provided by the invention can inhibit the growth of pathogenic bacteria pseudomonas elongata of white mold of morchella esculenta, and the bacteriostasis rate is 56.06%; meanwhile, the strain can promote formation of bacterial cream, the yield of morchella esculenta is increased by 20% or above, and the occurrence rate of white mold is reduced by 85% or above. The bacillus pumilus K40 provided by the invention is of great significance to the cultivation industry of morchella esculenta.

The application relates to the technical field of tail-cabbage waste resource utilization, in particular to a microbial agent and application thereof in the field of tail-cabbage fast rot. The microbial agent comprises Bacillus pumilus and Bacillus subtilis; the Bacillus subtilis is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 33122; the Bacillus pumilus is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 33121. The application also provides application of the microbial agent in the field of tail-cabbage fast rot. The application widens the tail-cabbage fast rot treatment method, enriches the microbial species that can be used for tail-cabbage fast rot, and can effectively improve the tail-cabbage rotting rate and the yield of the next crop by using the microbial agent provided by the application to carry out tail-cabbage fast rot.

The present application relates to the technical field of calcium carbonate manufacturing, and particularly relates to a preparation method of high-whiteness light calcium carbonate. The method comprises the following steps: efficient leaching of calcium element in carbide slag; preparation of microbial crystal form control agent; and electric dispersion treatment and precipitation of calcium ions. The microbial crystal form control agent is prepared by adjusting the mixing ratio of Bacillus pumilus fermentation liquor and Pseudomonas fluorescens fermentation liquor, so that Ca 2+ The stearic acid and sodium polyacrylate in the microbial crystal form control agent can make the particles more stable and provide ion adsorption capacity after the larger calcium carbonate particles are mineralized, CO2 is introduced after temperature rising, and the crystal form of the calcium carbonate is optimized, so that the vaterite type crystallization with smaller crystal form is formed. The Bacillus pumilus can also assist in generating small-particle-size calcium carbonate over time, so that the high-whiteness small-particle-size light calcium carbonate is obtained.

The application provides a bacillus pumilus and application thereof, the bacillus pumilus is preserved in Guangdong Microbial Culture Collection Center on July 24, 2025, and the preservation number is GDMCC No: 66749;The 16S rDNA of the bacillus pumilus is as shown in SEQ ID NO.1;The application of the bacillus pumilus is to prepare microbial preparation comprising the bacillus pumilus;The bacillus pumilus has good phosphorus dissolving capacity and IAA production capacity, can enhance the resistance of plants and promote the growth of plants.

The invention relates to the technical field of microorganisms and biological fertilizers, in particular to a vicia sativa growth-promoting and stress-resisting complex microbial inoculant and a preparation method thereof.The complex microbial inoculant is formed by mixing arthrobacter, bacillus pumilus, bacillus flexus and brevundimonas intermedia according to the proportion of the arthrobacter, the bacillus pumilus, the bacillus flexus and the brevundimonas intermedia, and the effective viable count in the complex microbial inoculant is 1 * 10 < 9 >-10 < 10 > CFU / g; the synthetic bacterial agent produced by the invention can be applied to the planting and production process of vicia sativa as bacterial fertilizer, and is convenient to apply and remarkable in growth promoting effect. The microbial agent not only can accelerate the accumulation of vicia sativa biomass and improve the production performance, but also can help vicia sativa to resist various environmental stresses, so that the viability of vicia sativa in a severe environment is guaranteed. The method has important significance for improving the production capacity of the common vetch and promoting the development of animal husbandry.

The application provides a compound preparation suitable for organic planting of tomatoes, a preparation method and application, and belongs to the technical field of organic tomato planting, wherein the active ingredient is composed of freshwater unicellular algae and a compound bacterial liquid; the compound bacterial liquid is composed of bacillus subtilis GLB191 and bacillus pumilus GLB197 or is composed of iranian gloeocystidiaceae; the effective viable bacterial number of the bacillus subtilis GLB191 and the bacillus pumilus GLB197 is greater than or equal to 13.5 billion cfu / g ‑1 ; and the propagule number of the iranian gloeocystidiaceae is greater than or equal to 70 pieces / ml ‑1 ; the application can improve the survival rate of tomato seedlings, plays a role in promoting growth, improves the quality of fruits, and has an effective prevention and treatment effect on tomato leaf mold disease.

The invention discloses a secondary salinization soil bioremediation method based on bacillus pumilus and application thereof, and belongs to the technical field of environmental protection. The problems that an existing microbial agent is low in nitrate tolerance, poor in conversion efficiency and limited in growth promoting capacity are solved. According to the technical scheme, the bacillus pumilus Y10 (the preservation number is CGMCC No.25291) has the nitrate tolerance concentration of 800-1200 mg.L <-1 >, nitrate larger than or equal to 500 mg.L <-1 > is converted by 80% or above within 18-24 hours, indoleacetic acid secreted by the bacillus pumilus Y10 is larger than or equal to 15 mg.L <-1 >, and siderophore secreted by the bacillus pumilus Y10 is larger than or equal to 20 mg.L <-1 >; according to the salt damage repairing and growth promoting integrated microbial inoculum, a strain and a humic acid carrier are mixed according to the weight ratio of 1: 9-1: 11 and are fixed through interaction of hydrogen bonds and Van der Waals' force, and the bonding strength is larger than or equal to 12 kJ / mol; according to the bioremediation method, the microbial agent is applied to secondary salinization soil with the nitrate content of 200-800 mg.kg <-1 >, the application depth is 10-15 cm, and the water content of the soil keeps the field moisture capacity of 60-70%.

The present invention relates to the use of a probiotic composition comprising the probiotic strains Lactobacillus plantarum DSM 33363, Lactobacillus plantarum DSM 33364, Lactobacillus paracasei DSM 33373, Lactobacillus reuteri DSM 33374, Bacillus megatherium DSM 33300, Bacillus pumilus DSM 33297, Bacillus pumilus DSM 33355 ("Promotrient" consortia) for the treatment and prevention of malnutrition in humans and animals, such as human beings and animals, such as human beings and animals. It increases the nutritional value of cereal foods, in particular from wheat, by increasing the bioavailability of essential micronutrients and amino acids contained in these foods.

The invention discloses algicidal bacteria TH41 and application thereof in inhibiting cyanobacterial bloom, and belongs to the technical field of biology. The algicidal bacterium TH41 is identified as bacillus pumilus TH41 and is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the algicidal bacterium TH41 is GDMCC No: 66948. The invention also provides an algicidal bacterial agent containing the algicidal bacteria TH41. The invention further provides application of the algicidal bacterium TH41 or the algicidal fungicide in inhibiting cyanobacterial bloom. The algicidal bacterium TH41 has a degradation effect on harmful blue-green algae, has an efficient blue-green algae inhibition effect, has biological safety on daphnia magna and zebra fish in an algae killing concentration range, is suitable for preventing and treating various blue-green algae such as microcystis aeruginosa, cuscuta larakii, pichia mold and anabaena pseudosciaena, and has a wide application prospect.

The invention relates to the technical field of biological fermentation, and discloses a functional fermentation product preparation process based on directional conversion of bean dregs, and the preparation process comprises the following steps: firstly, performing autolysis pretreatment on the bean dregs through an osmotic pressure regulator, activating an endogenous enzyme system, and releasing soluble nutrient substances; then, in the solid state fermentation stage, a composite stress factor composed of short-chain inulin and sorbitol is added, aspergillus oryzae is induced to form a loose and porous hypha network skeleton, and the ventilation and mass transfer efficiency of a fermentation substrate is remarkably improved. Then, regulating and controlling a cooling time sequence, adding L-glutamic acid as a metabolism inducer, and accurately receiving a metabolism path of bacillus megaterium to realize directional enrichment of functional products. The method effectively solves the problem that the physical structure is not matched with microbial metabolism in solid fermentation of the bean dregs, and improves the yield of functional fermentation products and the resource utilization value of the bean dregs.

The invention discloses screening and application of a salt-tolerant plant bacillus rhizosphere growth-promoting bacillus. The invention belongs to the technical field of biology, and particularly relates to screening and application of salt-tolerant plant growth-promoting bacillus. The bacillus pumilus disclosed by the invention is bacillus pumilus ZF526, and the preservation number of the bacillus pumilus is CGMCC (China General Microbiological Culture Collection Center) No.32942 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. Compared with other salt-tolerant strains, the bacillus pumilus provided by the research has a wider salt-tolerant range (most of other strains only tolerate NaCl or other single salts), and can adapt to salinized soil caused by different salts.

The application belongs to the technical field of soil conditioners, and particularly relates to a soil conditioner for improving fruit quality in orchards. The soil conditioner is prepared by mixing Bacillus thuringiensis TXB2-5 lyophilized powder, Bacillus pumilus TXB1-8 lyophilized powder and Bacillus altitudinis YLB2-1 lyophilized powder. The preservation number of the Bacillus thuringiensis TXB2-5 is CCTCC No: M 2020343, the preservation number of the Bacillus pumilus TXB1-8 is GDMCC No.62525, and the preservation number of the Bacillus altitudinis YLB2-1 is GDMCC No.62524. The soil conditioner is prepared by mixing Bacillus thuringiensis TXB2-5 lyophilized powder, Bacillus pumilus TXB1-8 lyophilized powder and Bacillus altitudinis YLB2-1 lyophilized powder, and can improve the control effect on diseases in orchards. In addition, the soil conditioner can improve the sugar content and vitamin C content of fruits in orchards, reduce the titratable acid content, and improve the fruit hardness, so that the fruit quality in orchards can be comprehensively improved, and the problem of single function of the conditioner can be effectively solved.

The invention relates to compositions comprising microbial strains and methods for use of the compositions in removing pollutants in waste, water, or soil, such as remediating dye and lignin, reducing contaminants, degrading paper (such as flushable or disposable or non-flushable wipes), reducing odor, reducing chemical oxygen demand (COD), and combinations thereof, in the water, the soil, or the waste or for use in administration to animals in the feed or drinking water of the animals. More particularly, the invention relates to compositions of isolated Bacillus strains selected from the group consisting of isolated Bacillus strains Bacillus licheniformis MDG-1000 (NRRL No. B-67888), Bacillus licheniformis MDG-1001 (NRRL No. B-67889), Bacillus subtilis / amyloliquefaciens MDG-8001 (NRRL No. B-67890), Bacillus pumilus MDG-1047 (NRRL No. B-67891), Bacillus amyloliquefaciens MDG1607 (NRRL No. B-67666), Bacillus subtilis MDGV18 (NRRL No. B-67665), Bacillus pumilus MDGV17 (NRRL No. B-67664), Bacillus subtilis MDG-1728 (NRRL No. B-67618), Bacillus megaterium MDG-2705 (NRRL No. B-67619), and strains having all of the identifying characteristics of these strains, and combinations thereof, and methods for use of these strains for removing pollutants in waste, water, or soil, such as remediating dye and lignin, reducing contaminants, degrading paper (such as flushable or disposable or non-flushable wipes), reducing odor, reducing chemical oxygen demand (COD), and combinations thereof, in the water, the soil, or the waste or for use in administration to animals in the feed or drinking water of the animals.

Compositions for the prevention and control of bacterial speck of tomato are described. The compositions comprises comprises an aqueous mixture of living Bacillus subtilis and living Bacillus pumilus. The compositions have been shown to be highly effective in the field. Also described are methods of use and kits comprising the compositions.

The invention provides a preparation method of a bacteria-algae composite preparation and application of the bacteria-algae composite preparation in degradation of nitrite in a water body. The compound preparation is prepared by taking bacillus pumilus HK77 (CCTCC (China Center for Type Culture Collection) NO: M20252511) as a core strain, and matching the core strain with a brown algae extract and a prebiotic carrier. Wherein the content of the bacillus pumilus is not lower than 5.00 * 10 CFU / g, the content of the brown algae extract is not lower than 300 mg / g, and the content of the prebiotics is not lower than 200 mg / g. The brown algae extract is obtained by carrying out synergistic enzymolysis on ascophyllum nodosum, gulfweed and / or kelp through brown algae lyase and fucoidin degrading enzyme, and the prebiotics are fructo-oligosaccharide and isomaltooligosacharide. The preparation is stable in a high-salinity environment, can significantly improve the nitrite degradation efficiency, and is suitable for the fields of environmental water quality improvement and aquaculture.