11 results about "Bacillus pumilus" patented technology

The application discloses a lactic acid bacteria and application thereof in straw-based feed protein, and belongs to the technical field of microorganisms. The application screens out bacillus pumilus and lactococcus lactis which can efficiently utilize straw. It is verified through experiments that after the bacillus pumilus and the lactococcus lactis are cultured in a fermentation liquor with wheat straw as the only carbon source for 5-7 days, the crude protein content is increased to 28.1% after fermentation, and the straw degradation rate is more than 88%, so that efficient conversion of the straw into high-quality feed protein is realized. Therefore, through construction of specific functional flora, optimization of a medium formula and a fermentation process, the application successfully realizes one-step efficient degradation of the straw and production of high-quality single-cell protein, and has the outstanding advantages of high degradation efficiency, high protein yield, stable colony, low cost and easy industrialization.

The application discloses application of a composite signal substance in promoting recruitment of rhizosphere beneficial microorganisms and relieving cadmium stress on leaves, and belongs to the technical field of biology, wherein the composite signal substance is L-valine and L-leucine, and the combination is obtained by detecting and identifying rice root exudates under leaf cadmium exposure through metabolomics. L-valine and L-leucine both present high abundance characteristics in indica rice and japonica rice root exudates; both of them can produce significant chemotaxis effect on bacillus subtillus and bacillus pumilus when used alone, and the synergistic effect is stronger after compounding, and the above-mentioned bacillus can be significantly induced to colonize in plant roots. The composite signal substance is applied to the rhizosphere soil of rice, and the relative abundance of bacillus in the soil can be effectively improved; pot experiment shows that the substance can significantly enhance the growth ability of rice under leaf cadmium stress by promoting the colonization of target bacillus, and has good field application and industrialization prospect.

The present invention relates to compositions having antimicrobial activity against Clavibacter michiganensis subsp michiganensis (“Cmm”). The compositions comprises Bacillus pumilus strain deposited with ATCC® Patent Designation No. PTA-125304, culture medium or extracts comprising Micrococcin P1 produced by said Bacillus pumilus strain, and / or isolated Micrococcin P1. Further provided herein are methods of making and using the antimicrobial compositions to protect and treat tomatoes from Cmm infections.

The present application belongs to the technical field of microorganisms, and particularly relates to a bacillus pumilus, a microbial agent, a method and application. The present application provides a bacillus pumilus G15 with a preservation number of CGMCC No.32839. The bacillus pumilus G15 of the present application has a significant inhibitory effect on a variety of pectobacterium FN20111. The present application further provides a microbial agent. Potting prevention effect determination shows that the bacillus pumilus G15 has a high prevention effect on the variety of pectobacterium FN20111.

The present application belongs to the technical field of bioengineering. The present application discloses a bacillus pumilus 21-14, the strain has been preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 6, 2024, the center is abbreviated as CGMCC, the strain preservation center registration number is CGMCC NO.31892, the strain preservation date is September 6, 2024, and the address of the preservation unit is No.3, Beichen West Road, Chaoyang District, Beijing. The bacillus pumilus strain 21-14 provided by the present application is simple to culture, short in cycle, free of ecological toxicity, rich in types of available carbon sources, strong in salt and alkali tolerance, capable of promoting the growth and development of potatoes through phosphorus solubilization and IAA production, beneficial to promoting the improvement of the biomass of crops in saline-alkali soil, and capable of effectively inhibiting the growth of streptomyces prolixus.

A hemp endophyte HDDMZ04 producing chrysoeridol and apigenin and application thereof relates to the field of microorganisms, and aims to solve the problems of high cost and environmental unfriendliness of the existing preparation method of chrysoeridol and apigenin. The hemp endophyte is fusarium HDDMZ04, which has been preserved in China typical culture collection center, the preservation address is Wuhan University in Wuhan, the preservation date is December 25, 2025, and the preservation number is CCTCC NO: M 20253016. The hemp endophyte can ferment to produce chrysoeridol and apigenin, has in-vitro antioxidant effect, and has inhibitory effect on bacillus subtilis, bacillus pumilus and pseudomonas aeruginosa. The present application is used for bacteriostasis, production of chrysoeridol and apigenin.

This invention discloses a high-yield 1-deoxynojirimycin-producing *Bacillus pumilus* strain and its applications, belonging to the field of microbiology. This invention, through screening, ARTP and NTG mutagenesis, obtained a *Bacillus pumilus* strain with significantly increased 1-deoxynojirimycin production. Bacillus pumilus DNJ-BP01, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38218, was used to inoculate the seed culture of Bacillus pumilus DNJ-BP01 into a fermentation medium containing 5% beef extract supplemented with 2.5% mannitol as the carbon source and 3% potassium nitrate as the nitrogen source. After 76 hours of aerobic fermentation, the yield of 1-deoxynojirimycin reached 0.892 g / L. This invention provides a new fermentation strain for the efficient industrial production of 1-deoxynojirimycin and has broad application prospects.

This invention belongs to the field of fermentation engineering, specifically relating to a pH control process for the production of γ-poly(diaminobutyric acid) by Bacillus pumilus. The pH control process of this invention includes: firstly, maintaining the pH of the fermentation broth above 6.0 for 8-15 hours to allow its OD (oxidative stress) to reach a certain level. 600 The pH value reaches above 20; then, without pH control, it is allowed to decrease naturally. When it drops to 4.5±0.1, it is maintained for 8-12 hours; then, without pH control, it is allowed to decrease naturally again to 3.5±0.1, maintained for 2-4 hours, and then the pH is raised to 4.2±0.1 and maintained for 4-8 hours; then, the pH fluctuates between 4.1 and 3.8 according to dissolved oxygen conditions until fermentation ends. This invention solves the problem of low γ-PAB yield caused by low fermentation levels in existing methods, and features a simple control method and high yield, reaching up to 6900 mg / L.

The present application relates to a kind of microbial pretreatment tobacco waste pyrolysis preparation spice process, comprising the following steps: S1, strain selection: bacillus subtilis V16 strain is aimed at tobacco stem, bacillus pumilus V35 strain is aimed at tobacco leaf, trichoderma viride CE1 is aimed at tobacco dust;S2, preparation of microbial preparation;S3, microbial pretreatment: preparation is separately sprayed on tobacco stem, tobacco leaf, tobacco dust, then is loaded into sealed bag, under certain temperature and humidity conditions, after a certain time, high-temperature inactivation and drying;S4, grinding dry distillation extraction bio-oil: after the pretreatment of tobacco waste drying and grinding, it is placed in reactor and carries out dry distillation reaction, after a certain time under the dry distillation temperature set, obtain bio-oil and biochar.The present application uses microbial target pretreatment technology, optimizes pyrolysis reaction path and improves the selectivity of product, provides solid theoretical basis and technical reference for high-value utilization of tobacco waste.

This invention relates to the field of Bacillus pumilus, addressing the problems of poor conversion rate, purity, and product stability in existing biological methods for preparing nano-selenium. It provides a Bacillus pumilus strain and its applications, as well as a method for preparing bio-nano-selenium using Bacillus pumilus. The Bacillus pumilus strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO. 36324. The Bacillus pumilus strain provided by this invention can tolerate high concentrations of sodium selenite and exhibits high conversion rate and good stability, solving the technical problems of difficult bacterial growth, low conversion efficiency, and poor stability under high selenium stress. Furthermore, the method of this invention has low equipment requirements, a simple process, low cost, high product purity, and is environmentally friendly. The product combines the high bioactivity of nano-selenium with the absorption-promoting and anti-inflammatory properties of polysaccharides, and can be widely used in feed additives and plant growth regulators.