87 results about "Bacillus pumilus" patented technology

The invention relates to the technical field of agricultural planting, in particular to a method for preventing and treating watermelon fusarium wilt by using a green bactericide, which comprises the following steps: preparing the green bactericide which comprises the following components in percentage by mass: 18-25% of composite plant source active component consisting of water lettuce extract, enteromorpha polysaccharide and field horsetail flavone; 20%-30% of an antagonistic bacterium compound preparation, wherein the antagonistic bacterium compound preparation comprises bacillus pumilus and trichoderma; 38%-45% of a nano-carrier, wherein a chitosan-sodium alginate compound is used as an embedding material; and the balance of auxiliary components. Applying the green bactericide to watermelon plants; through component optimization and a synergistic effect, compared with the prior art, the composition has the remarkable advantages that the safety is high, all the components are from natural plants or microorganisms, no chemical pesticide residue exists, the composition is healthy to human bodies and environmentally friendly, and the problems of drug resistance and soil pollution caused by chemical agents can be avoided.

The invention discloses a salt-tolerant brevibacillus for promoting growth and preventing and controlling plant diseases and application of the salt-tolerant brevibacillus. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025, and the preservation number of the brevibacillus halotolerans T101 is GDMCC No: 66731. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025. The strain T101 disclosed by the invention can effectively antagonize plant pathogenic fungi peronophythora litchii and plant pathogenic bacteria such as pseudomonas syringae, xanthomonas campestris, citrus canker pathogen and ralstonia solanacearum, can generate siderophores and plant growth hormone IAA, and can remarkably promote the growth of tomato root systems in a pot experiment. Therefore, the strain T101 can be used for preparing microbial preparation products for preventing and treating plant diseases and promoting plant root growth, and has important application potential in the aspects of microbial agents, biological fertilizers and the like for promoting plant growth and development.

The invention discloses a strain NJSH56 for promoting plant growth and application thereof, the strain NJSH56 is identified as bacillus pumilus, the strain NJSH56 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation time is November 14, 2024, and the strain preservation number is CGMCC NO.32614. The strain NJSH56 can promote plant growth. The dendrobium officinale endophytic growth-promoting strain NJSH56 for promoting plant growth is obtained through separation and screening, growth of dendrobium officinale tissue culture seedlings can be effectively promoted, and the fresh weight, the rooting number and the leaf number are increased; greenhouse tests show that the number of new buds of dendrobium officinale plants in a strain NJSH56 treatment group reaches 4.67 and is obviously higher than that of a control group; meanwhile, the NJSH56 is used for treating the non-host plant Chinese cabbage, so that the growth of the overground part of the Chinese cabbage can be remarkably promoted, and the biomass accumulation of the Chinese cabbage is improved.

The invention belongs to the technical field of microbial agents, and particularly relates to a microbial agent containing bacillus velezensis and application of the microbial agent to astragalus membranaceus. The invention discloses a microbial agent for astragalus membranaceus. The microbial agent is prepared by mixing bacillus velezensis powder, bacillus subtilis powder and bacillus pumilus powder according to the mass ratio of 1: (1-10): (1-5), the bacterium content of the bacillus velezensis powder, the bacillus subtilis powder and the bacillus pumilus powder is 0.2-0.5 billion cfu / g. The bacillus velezensis, the bacillus subtilis and the bacillus pumilus are fermented respectively, fermentation liquor is taken to be prepared into powder, and the powder is mixed according to a certain mass ratio to form the compound microbial agent, so that the compound microbial agent can improve the prevention and treatment effect on the radix astragali root knot nematode disease, is green and environment-friendly, can effectively overcome the defects of chemical agents, and has a good application prospect. Support is provided for biological prevention and control of the radix astragali root knot nematode disease, and meanwhile healthy growth of radix astragali plants can be guaranteed.

The invention provides a compound feed additive for relieving stress response of marine fishes, which is prepared by the following steps: mixing raw materials such as hermetia illucens protein and the like to prepare a premix, and embedding the premix by using a lac-glycyrrhizic acid system to prepare lac microcapsules; then modifying the fucoidin by adopting glycyrrhizin and gamma-linolenic acid, embedding rhodosporidium palustris and bacillus pumilus by using the fucoidin to prepare compound bacterium nano-particles, and finally mixing the lac microcapsules and the compound bacterium nano-particles in proportion. According to the invention, excessive secretion of cortisol of marine fish can be effectively inhibited, hermetia illucens protein provides rich nutrition and reduces muscle decomposition, and compound microorganisms are beneficial to maintaining intestinal microecological balance and improving the utilization rate of nutrition. The prepared compound feed additive can be directly splashed into a culture or transportation water body or added into a feed, so that the stress reaction of the marine fish is effectively relieved, the survival rate of the marine fish is increased, and powerful support is provided for culture of the marine fish.

The present disclosure relates generally to compositions comprising Bacillus pumilus having a positive impact on animal performance parameters, the use of Bacillus pumilus for preventing, reducing or controlling bacterial colonization or infection, a method of improving one or more animal performance parameters, and a kit. In particular, the present disclosure relates to Bacillus pumilus DSM 34300 and DSM 34299.

The application relates to the technical field of tail-cabbage waste resource utilization, in particular to a microbial agent and application thereof in the field of tail-cabbage fast rot. The microbial agent comprises Bacillus pumilus and Bacillus subtilis; the Bacillus subtilis is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 33122; the Bacillus pumilus is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 33121. The application also provides application of the microbial agent in the field of tail-cabbage fast rot. The application widens the tail-cabbage fast rot treatment method, enriches the microbial species that can be used for tail-cabbage fast rot, and can effectively improve the tail-cabbage rotting rate and the yield of the next crop by using the microbial agent provided by the application to carry out tail-cabbage fast rot.

The present application relates to the technical field of calcium carbonate manufacturing, and particularly relates to a preparation method of high-whiteness light calcium carbonate. The method comprises the following steps: efficient leaching of calcium element in carbide slag; preparation of microbial crystal form control agent; and electric dispersion treatment and precipitation of calcium ions. The microbial crystal form control agent is prepared by adjusting the mixing ratio of Bacillus pumilus fermentation liquor and Pseudomonas fluorescens fermentation liquor, so that Ca 2+ The stearic acid and sodium polyacrylate in the microbial crystal form control agent can make the particles more stable and provide ion adsorption capacity after the larger calcium carbonate particles are mineralized, CO2 is introduced after temperature rising, and the crystal form of the calcium carbonate is optimized, so that the vaterite type crystallization with smaller crystal form is formed. The Bacillus pumilus can also assist in generating small-particle-size calcium carbonate over time, so that the high-whiteness small-particle-size light calcium carbonate is obtained.

The application provides a bacillus pumilus and application thereof, the bacillus pumilus is preserved in Guangdong Microbial Culture Collection Center on July 24, 2025, and the preservation number is GDMCC No: 66749;The 16S rDNA of the bacillus pumilus is as shown in SEQ ID NO.1;The application of the bacillus pumilus is to prepare microbial preparation comprising the bacillus pumilus;The bacillus pumilus has good phosphorus dissolving capacity and IAA production capacity, can enhance the resistance of plants and promote the growth of plants.

The application provides a compound preparation suitable for organic planting of tomatoes, a preparation method and application, and belongs to the technical field of organic tomato planting, wherein the active ingredient is composed of freshwater unicellular algae and a compound bacterial liquid; the compound bacterial liquid is composed of bacillus subtilis GLB191 and bacillus pumilus GLB197 or is composed of iranian gloeocystidiaceae; the effective viable bacterial number of the bacillus subtilis GLB191 and the bacillus pumilus GLB197 is greater than or equal to 13.5 billion cfu / g ‑1 ; and the propagule number of the iranian gloeocystidiaceae is greater than or equal to 70 pieces / ml ‑1 ; the application can improve the survival rate of tomato seedlings, plays a role in promoting growth, improves the quality of fruits, and has an effective prevention and treatment effect on tomato leaf mold disease.

The invention discloses a secondary salinization soil bioremediation method based on bacillus pumilus and application thereof, and belongs to the technical field of environmental protection. The problems that an existing microbial agent is low in nitrate tolerance, poor in conversion efficiency and limited in growth promoting capacity are solved. According to the technical scheme, the bacillus pumilus Y10 (the preservation number is CGMCC No.25291) has the nitrate tolerance concentration of 800-1200 mg.L <-1 >, nitrate larger than or equal to 500 mg.L <-1 > is converted by 80% or above within 18-24 hours, indoleacetic acid secreted by the bacillus pumilus Y10 is larger than or equal to 15 mg.L <-1 >, and siderophore secreted by the bacillus pumilus Y10 is larger than or equal to 20 mg.L <-1 >; according to the salt damage repairing and growth promoting integrated microbial inoculum, a strain and a humic acid carrier are mixed according to the weight ratio of 1: 9-1: 11 and are fixed through interaction of hydrogen bonds and Van der Waals' force, and the bonding strength is larger than or equal to 12 kJ / mol; according to the bioremediation method, the microbial agent is applied to secondary salinization soil with the nitrate content of 200-800 mg.kg <-1 >, the application depth is 10-15 cm, and the water content of the soil keeps the field moisture capacity of 60-70%.

The invention discloses algicidal bacteria TH41 and application thereof in inhibiting cyanobacterial bloom, and belongs to the technical field of biology. The algicidal bacterium TH41 is identified as bacillus pumilus TH41 and is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the algicidal bacterium TH41 is GDMCC No: 66948. The invention also provides an algicidal bacterial agent containing the algicidal bacteria TH41. The invention further provides application of the algicidal bacterium TH41 or the algicidal fungicide in inhibiting cyanobacterial bloom. The algicidal bacterium TH41 has a degradation effect on harmful blue-green algae, has an efficient blue-green algae inhibition effect, has biological safety on daphnia magna and zebra fish in an algae killing concentration range, is suitable for preventing and treating various blue-green algae such as microcystis aeruginosa, cuscuta larakii, pichia mold and anabaena pseudosciaena, and has a wide application prospect.

The invention relates to the technical field of biological fermentation, and discloses a functional fermentation product preparation process based on directional conversion of bean dregs, and the preparation process comprises the following steps: firstly, performing autolysis pretreatment on the bean dregs through an osmotic pressure regulator, activating an endogenous enzyme system, and releasing soluble nutrient substances; then, in the solid state fermentation stage, a composite stress factor composed of short-chain inulin and sorbitol is added, aspergillus oryzae is induced to form a loose and porous hypha network skeleton, and the ventilation and mass transfer efficiency of a fermentation substrate is remarkably improved. Then, regulating and controlling a cooling time sequence, adding L-glutamic acid as a metabolism inducer, and accurately receiving a metabolism path of bacillus megaterium to realize directional enrichment of functional products. The method effectively solves the problem that the physical structure is not matched with microbial metabolism in solid fermentation of the bean dregs, and improves the yield of functional fermentation products and the resource utilization value of the bean dregs.

The invention discloses screening and application of a salt-tolerant plant bacillus rhizosphere growth-promoting bacillus. The invention belongs to the technical field of biology, and particularly relates to screening and application of salt-tolerant plant growth-promoting bacillus. The bacillus pumilus disclosed by the invention is bacillus pumilus ZF526, and the preservation number of the bacillus pumilus is CGMCC (China General Microbiological Culture Collection Center) No.32942 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. Compared with other salt-tolerant strains, the bacillus pumilus provided by the research has a wider salt-tolerant range (most of other strains only tolerate NaCl or other single salts), and can adapt to salinized soil caused by different salts.

The application belongs to the technical field of soil conditioners, and particularly relates to a soil conditioner for improving fruit quality in orchards. The soil conditioner is prepared by mixing Bacillus thuringiensis TXB2-5 lyophilized powder, Bacillus pumilus TXB1-8 lyophilized powder and Bacillus altitudinis YLB2-1 lyophilized powder. The preservation number of the Bacillus thuringiensis TXB2-5 is CCTCC No: M 2020343, the preservation number of the Bacillus pumilus TXB1-8 is GDMCC No.62525, and the preservation number of the Bacillus altitudinis YLB2-1 is GDMCC No.62524. The soil conditioner is prepared by mixing Bacillus thuringiensis TXB2-5 lyophilized powder, Bacillus pumilus TXB1-8 lyophilized powder and Bacillus altitudinis YLB2-1 lyophilized powder, and can improve the control effect on diseases in orchards. In addition, the soil conditioner can improve the sugar content and vitamin C content of fruits in orchards, reduce the titratable acid content, and improve the fruit hardness, so that the fruit quality in orchards can be comprehensively improved, and the problem of single function of the conditioner can be effectively solved.

Compositions for the prevention and control of bacterial speck of tomato are described. The compositions comprises comprises an aqueous mixture of living Bacillus subtilis and living Bacillus pumilus. The compositions have been shown to be highly effective in the field. Also described are methods of use and kits comprising the compositions.

The invention provides a preparation method of a bacteria-algae composite preparation and application of the bacteria-algae composite preparation in degradation of nitrite in a water body. The compound preparation is prepared by taking bacillus pumilus HK77 (CCTCC (China Center for Type Culture Collection) NO: M20252511) as a core strain, and matching the core strain with a brown algae extract and a prebiotic carrier. Wherein the content of the bacillus pumilus is not lower than 5.00 * 10 CFU / g, the content of the brown algae extract is not lower than 300 mg / g, and the content of the prebiotics is not lower than 200 mg / g. The brown algae extract is obtained by carrying out synergistic enzymolysis on ascophyllum nodosum, gulfweed and / or kelp through brown algae lyase and fucoidin degrading enzyme, and the prebiotics are fructo-oligosaccharide and isomaltooligosacharide. The preparation is stable in a high-salinity environment, can significantly improve the nitrite degradation efficiency, and is suitable for the fields of environmental water quality improvement and aquaculture.

The invention discloses a crop straw rapid decomposition bacterial agent for sandy soil and a fermentation process thereof, and particularly relates to the field of agricultural microorganisms, and the bacterial agent is prepared by compounding trichoderma viride, bacillus pumilus, trichoderma longibrachiatum and bacillus subtilis according to the volume ratio of 1: 1: 2: 2. According to the preparation process, a solid culture medium taking straw as a main material is adopted, and mixed solid fermentation is performed through layered inoculation, staged variable temperature control, humidity regulation and intermittent ventilation, so that the final product has high viable count and strong enzyme activity, and can keep high activity in a severe environment of sandy soil; the straw decomposition process is quickly started and completed; the microbial inoculum can efficiently and rapidly decompose crop straws in sandy soil, significantly improve humification efficiency and soil fertility, effectively improve soil organic matter and nutrient content, improve soil structure and promote crop yield increase, and has important application value and market prospect.

The invention discloses application of bacillus pumilus YYZB12 in growth promoting cultivation of medicinal rhubarb, and belongs to the technical field of microorganisms. The bacillus pumilus YYZB12 strain is separated from rhizosphere soil of medicinal rhubarb in Zhenban county in Han City of Shaanxi Province, namely a genuine production area of medicinal rhubarb, and is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.35249. The strain has efficient phosphate solubilizing capacity, can promote the thickening growth of the main root of the medicinal part of the medicinal rhubarb, and obviously improves the accumulation of anthraquinone secondary metabolites at the root of the medicinal rhubarb. An excellent microbial strain resource is provided for ecological planting of the medicinal rhubarb, the method can be applied to artificial cultivation of the medicinal rhubarb, the medicinal material quality and the commodity value of the medicinal rhubarb are effectively improved, and the method has important significance in promoting reasonable application of a microbial technology in standardized planting of medicinal plants.

The invention discloses a functional microbial agent for near-natural recovery of moderately-degraded high-cold grassland as well as a preparation method and application of the functional microbial agent. The composite bacterial agent is prepared by mixing bacterial culture solutions of four bacterial strains, namely bacillus pumilus E-B-2-B05 (CGMCC No. 21387), paenibacillus silage P-B-2-B03 (CGMCC No. 21386), bacillus subtilis P-B-4-SCB23 (CGMCC No. 27084) and bacillus velezensis K-B-5-SCB13 (CGMCC No. 27088) according to the volume ratio of 3: 2.5: 2: 2.5. The invention further discloses a preparation method of the composite bacterial agent. Through the synergistic effect of multiple strains, the complex microbial inoculant can effectively promote plant growth, enhance rhizosphere microbial activity and improve the ecological process of soil, can effectively improve grassland biomass, optimize a plant community structure, enhance competitive advantages of high-quality pasture, and promote moderately-degraded alpine grassland vegetation to recover to a near-natural state. The microbial agent is simple in preparation method, convenient and flexible to apply and environment-friendly, and has good ecological and economic benefits.

The invention relates to the technical field of compost and microbial inoculum preparation, and provides a method for preparing compound bacteria through high-temperature rapid compost maturing of sugarcane leaves and cow dung, and the method comprises the following steps: step 1, raw material pretreatment: crushing the sugarcane leaves to particle size, drying the cow dung to water content, mixing the sugarcane leaves and the cow dung according to a mass ratio of 3: 1-5: 1, and sterilizing; step 2, preparing an initial bacterial liquid: inoculating a composite strain into a culture medium, and culturing for 24-48 hours at 35-40 DEG C under the condition that the rotating speed of a shaking table is 150-180rpm to obtain the initial bacterial liquid; the composite strains comprise bacillus siamensis and bacillus pumilus, and the ratio of the strains is 2: 1; and step 3, high-temperature composting and maturing. Agricultural waste recycling is achieved by treating sugarcane leaves and cow dung, pollution is reduced, and low-cost raw materials are provided; a dynamic temperature control and forced ventilation process and a specific strain are adopted, so that decomposition is accelerated, and the activity of the microbial inoculum is improved; the system achieves modularized operation, achieves whole-process standardization and automatic control, and improves the efficiency and stability.

The present disclosure relates to compositions and methods for cleaning and / or odor control. More particularly, the present disclosure relates to compositions comprising a brevibacillus laterosporus fermentation extract or fractions thereof for cleaning and / or odor control and methods of using the same. Compositions containing brevibacillus laterosporus ferment extracts are suitable for use as detergents and for cleaning and odor control of fabrics and hard surfaces, as well as for a variety of other applications.

The invention belongs to the technical field of microorganisms, and particularly relates to a composite microbial preparation and a preparation method thereof.The composite microbial preparation comprises an inner core, a middle layer and an outer layer; the inner core is an agricultural microorganism, the middle layer is a metal phenolic network layer, and the outer layer is a wrapping layer; the agricultural microorganisms comprise bacillus cereus and / or bacillus pumilus; the viable concentration of the agricultural microorganisms is greater than or equal to 1 * 10 < 12 > CFU / mL; and the metal phenolic network layer is prepared from the following raw materials: polyphenol and ferric salt. The compound microbial preparation is stable in property, has good slow release and degradation effects, can be preserved for a long time, does not need a cold chain in the storage or transportation process, and is convenient to carry and transport.

The present application relates to a kind of acne-removing composition and its preparation method and application, belong to the field of cosmetics technology.The acne-removing composition of the present application includes Gentiana straminea Maxim extract, biota orientalis leaf extract and norway spruce leaf extract, wherein Gentiana straminea Maxim extract is prepared by ultrasonic treatment after mixed bacteria fermentation of Gentiana straminea Maxim as raw material by Kluyveromyces marxianus and Bacillus pumilus, and the obtained Gentiana straminea Maxim extract has improved anti-inflammatory effect.The Gentiana straminea Maxim extract prepared in the present application is compounded with biota orientalis leaf extract and norway spruce leaf extract, and through determination, the three can synergistically improve the inhibitory effect of acne-removing composition on propionibacterium acnes, and simultaneously, the acne-removing composition of the present application can exert acne-removing effect from anti-inflammatory and bacteriostatic, oil control and other aspects, reduce facial acne area, reduce facial inflammation and reduce oil secretion.

The invention provides a bacillus brevis Sys-Hup-008 strain for producing huperzine A. The strain is preserved in the China Center for Type Culture Collection on October 31, 2025, the preservation number is CCTCC NO: M20252403, the preservation number is CCTCC NO: M20252403, the preservation number is CCTCC NO: M20252404, and the preservation number is CCTCC NO: M20252404. The 16S rDNA (ribosomal deoxyribonucleic acid) sequence of the strain is as shown in SEQ ID NO. 1. The bacillus brevis Sys-Hup-008 strain provided by the invention can produce huperzine A through metabolism, can be subjected to large-scale fermentation culture in a short time, is relatively low in fermentation cost, is not limited by conditions such as time domain, season and the like, and has a good application prospect. Therefore, the problems that the chemical synthesis cost of huperzine A is relatively high, the steps are complicated and more intermediate pollutants exist can be solved through a microbial fermentation way. Meanwhile, the invention also provides optimized microbial fermentation conditions and a formula of the culture medium, and the yield of huperzine A is relatively high.

A bacterial composition and its use for the control, inhibition and / or prevention of Corynebacterium Michaeli subsp. Michaeli (Cmm) infection in tomatoes are described. The bacterial composition comprises an aqueous mixture of live Bacillus subtilis and live Bacillus pumilus. The bacterial compositions may be used in combination with existing herbicides, insecticides, fungicides, fungicides, and nutritional agents, including but not limited to copper-containing compounds. Synergistic effects are provided in combination with copper fungicides, such as Kocide.

The application provides ferulic acid esterase mutants with improved specific activity and application thereof, and the application carries out directional evolution on a ferulic acid esterase gene derived from Bacillus pumilus, expresses the ferulic acid esterase gene in an E. coli expression system, and obtains ferulic acid esterase mutants DV1 and DV2 with improved enzyme activity and expression efficiency through screening. The amino acid sequences of the ferulic acid esterase mutants DV1 and DV2 are shown in SEQ ID NO. 4 and SEQ ID NO. 6 respectively, and the mutants DV1 and DV2 obtained by the application have higher protein yield, higher specific activity of methyl ferulate esterase, and higher degradation efficiency of macromolecular p-nitrophenol ester substrates compared with the original bacteria BpFAE. Therefore, the mutants can be used for degrading plant fibers and preparing feed fermenting agents, and have good market application prospect.

The application discloses a lactic acid bacteria and application thereof in straw-based feed protein, and belongs to the technical field of microorganisms. The application screens out bacillus pumilus and lactococcus lactis which can efficiently utilize straw. It is verified through experiments that after the bacillus pumilus and the lactococcus lactis are cultured in a fermentation liquor with wheat straw as the only carbon source for 5-7 days, the crude protein content is increased to 28.1% after fermentation, and the straw degradation rate is more than 88%, so that efficient conversion of the straw into high-quality feed protein is realized. Therefore, through construction of specific functional flora, optimization of a medium formula and a fermentation process, the application successfully realizes one-step efficient degradation of the straw and production of high-quality single-cell protein, and has the outstanding advantages of high degradation efficiency, high protein yield, stable colony, low cost and easy industrialization.

The application discloses application of a composite signal substance in promoting recruitment of rhizosphere beneficial microorganisms and relieving cadmium stress on leaves, and belongs to the technical field of biology, wherein the composite signal substance is L-valine and L-leucine, and the combination is obtained by detecting and identifying rice root exudates under leaf cadmium exposure through metabolomics. L-valine and L-leucine both present high abundance characteristics in indica rice and japonica rice root exudates; both of them can produce significant chemotaxis effect on bacillus subtillus and bacillus pumilus when used alone, and the synergistic effect is stronger after compounding, and the above-mentioned bacillus can be significantly induced to colonize in plant roots. The composite signal substance is applied to the rhizosphere soil of rice, and the relative abundance of bacillus in the soil can be effectively improved; pot experiment shows that the substance can significantly enhance the growth ability of rice under leaf cadmium stress by promoting the colonization of target bacillus, and has good field application and industrialization prospect.

This document describes a bacterial composition and its use for controlling, suppressing, and / or preventing infection of tomatoes with the tomato canker fungus (Cmm). The bacterial composition comprises an aqueous mixture of live Bacillus subtilis and live Bacillus pumilus. The bacterial composition can be combined with existing herbicides, insecticides, mycicides, bactericidal agents, and nutrients, including but not limited to copper-containing compounds. Combination with copper mycicides such as Kocide® imparts synergistic efficacy.