155 results about "Bacillus pumilus" patented technology

The invention relates to a complex microbial inoculant for preventing and treating citrus huanglongbing as well as a preparation method and application of the complex microbial inoculant. The compound bacterial agent for preventing and treating the citrus huanglongbing is prepared from the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. The compound bacterial agent for preventing and treating the citrus huanglongbing comprises the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. After the complex microbial inoculant is added, the content of callose in infected leaves can be effectively reduced, the self-resistance mechanism of plants is induced, the disease resistance is improved, and the complex microbial inoculant has an obvious inhibiting effect and a growth promoting effect on Candidatus Liberibacter asiaticum.

The invention relates to bacillus pumilus and application thereof. The bacillus pumilus DX-fK is obtained through a chemical mutagenesis method, the bacillus pumilus DX-fK can efficiently degrade straw, the bacillus pumilus DX-fK is used as a substrate to produce feed alternative protein, and therefore the bacillus pumilus DX-fK can be applied industrially and has good industrialization prospects.

The invention provides bacillus pumilus and application thereof, and belongs to the technical field of microorganisms, the bacillus pumilus YM3-12 provided by the invention is preserved in China General Microbiological Culture Collection Center on October 21, 2024, and the preservation number is CGMCC No. 32281; the strain is high in salt and alkali resistance, has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, dissolving potassium, degrading protein, degrading cellulose, producing iron carriers and the like, can antagonize Erwinia persicae and fusarium oxysporum, has an obvious growth promoting effect on wheat, radishes and cucumbers in a salt and alkali environment, and is very wide in application prospect.

The invention provides a complex microbial inoculant as well as a preparation method and application thereof, and relates to the technical field of complex microbial inoculant preparation. The compound bacterial agent is prepared from bacillus pumilus JMB004, bacillus safeensis strain BXC22 and bacillus sp. EB35, and the compound bacterial agent is prepared from bacillus pumilus strain JMB004, bacillus safeensis strain BXC22 and bacillus sp. EB35. The three strains are obtained through autonomous screening and prepared into the complex microbial inoculant, dry powder can be stored at normal temperature, the storage life is prolonged to one year, and the composite microbial inoculant is convenient to carry. The complex microbial inoculant is only composed of three strains of bacteria, and the components are simple; the low-temperature composting agent is applied to composting treatment under a low-temperature condition, and is relatively high in temperature rise rate, relatively short in fermentation time and relatively high in decomposition degree.

The invention relates to the technical field of saline-alkali soil improvement and microorganisms, in particular to bacillus pumilus S3-9 and application thereof. The bacillus pumilus S3-9 is preserved in the China General Microbiological Culture Collection Center on December 19, 2024, and the preservation number of the bacillus pumilus S3-9 is CGMCC (China General Microbiological Culture Collection Center) No. 33120. The bacillus pumilus S3-9 provided by the invention has the effects of reducing salinity in saline-alkali soil, increasing nutrients of the saline-alkali soil and improving the diversity and stability of rhizosphere microbial communities of the saline-alkali soil, and can be applied to improving soil fertility, promoting nutrient circulation, improving soil quality, relieving soil salinization and the like. The strain can also be used for increasing the yield of sweet sorghum in saline-alkali land, has a remarkable growth promoting effect on plant height, overground part fresh weight and underground part fresh weight, can effectively improve the K < + > / Na < + > ratio, chlorophyll content, superoxide dismutase activity and catalase activity, and is beneficial to enhancing the adaptive capacity of plants to salt stress.

The invention discloses bacillus pumilus, a complex microbial inoculant and application of the complex microbial inoculant in a saline-alkali soil four-dimensional improvement method, and belongs to the technical field of microorganism application. According to the invention, a strain of bacillus pumilus is screened, and the bacillus pumilus has excellent saline-alkaline tolerance, has multiple functions of producing organic acid, proline, siderophore, trehalose, IAA and betaine and the like, and has obvious advantages in saline-alkali soil improvement; the stress resistance of plants is improved through multiple mechanisms, nutrient absorption is promoted, the soil structure and chemical environment are improved, the stability and diversity of soil microbial communities are enhanced, and then effective treatment on the saline-alkali soil is achieved. According to the four-dimensional improvement method for the saline-alkali soil, the microbial technology, the physical technology, the chemical technology and the nutrition technology are combined, the saline-alkali soil is improved from the four dimensions of physics, chemistry, biology and nutrition, soil can be effectively improved, and repeated planting is not likely to happen.

The invention relates to the technical field of agricultural planting, in particular to a method for preventing and treating watermelon fusarium wilt by using a green bactericide, which comprises the following steps: preparing the green bactericide which comprises the following components in percentage by mass: 18-25% of composite plant source active component consisting of water lettuce extract, enteromorpha polysaccharide and field horsetail flavone; 20%-30% of an antagonistic bacterium compound preparation, wherein the antagonistic bacterium compound preparation comprises bacillus pumilus and trichoderma; 38%-45% of a nano-carrier, wherein a chitosan-sodium alginate compound is used as an embedding material; and the balance of auxiliary components. Applying the green bactericide to watermelon plants; through component optimization and a synergistic effect, compared with the prior art, the composition has the remarkable advantages that the safety is high, all the components are from natural plants or microorganisms, no chemical pesticide residue exists, the composition is healthy to human bodies and environmentally friendly, and the problems of drug resistance and soil pollution caused by chemical agents can be avoided.

The invention discloses a salt-tolerant brevibacillus for promoting growth and preventing and controlling plant diseases and application of the salt-tolerant brevibacillus. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025, and the preservation number of the brevibacillus halotolerans T101 is GDMCC No: 66731. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025. The strain T101 disclosed by the invention can effectively antagonize plant pathogenic fungi peronophythora litchii and plant pathogenic bacteria such as pseudomonas syringae, xanthomonas campestris, citrus canker pathogen and ralstonia solanacearum, can generate siderophores and plant growth hormone IAA, and can remarkably promote the growth of tomato root systems in a pot experiment. Therefore, the strain T101 can be used for preparing microbial preparation products for preventing and treating plant diseases and promoting plant root growth, and has important application potential in the aspects of microbial agents, biological fertilizers and the like for promoting plant growth and development.

The invention relates to the technical field of agricultural biology, and discloses a microbial agent for preventing and treating fructus aurantii scab and a preparation method of the microbial agent. The microbial agent comprises the following raw materials in percentage by weight: 10-13% of paenibacillus mucilaginosus; 15%-20% of trichoderma sp. 7%-10% of paecilomyces lilacinus; 5%-8% of bacillus pumilus; 7%-9% of mycorrhizal fungi; 5%-8% of streptomyces; 5%-9% of a coptis chinensis extract; 4%-7% of a sophora flavescens extract; 2%-4% of realgar; 1%-3% of a rheum officinale extract; 10%-13% of soybean meal; 5%-8% of a water-soluble fertilizer; 7%-9% of potassium fulvate; 1%-3% of chitosan; 0.5%-1% of trace elements; 3%-8% of humic acid; the raw materials are subjected to microbial flora amplification culture, plant antibacterial component light-shielding mixing and low-temperature mixing to effectively protect the microbial activity of the microbial agent, and step-by-step operation can avoid damage of high temperature and mechanical shearing to microorganisms, so that the microbial activity of the microbial agent is effectively protected. And the loss of microbial active ingredients is reduced by vacuum drying and low-temperature granulation processes.

The invention relates to the technical field of resource utilization of rotten vegetable waste, in particular to a microbial agent and application thereof in the field of rapid rotting of rotten vegetable waste. The microbial agent comprises bacillus pumilus and bacillus subtilis, bacillus subtilis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33122; the bacillus pumilus is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 33121. The invention also provides an application of the microbial agent in the field of rapid rotting of rotten vegetable leaves. The treatment method for rapid rotting of the rotten vegetable leaves is widened, the types of microorganisms capable of being used for rapid rotting of the rotten vegetable leaves are enriched, the rotting rate of the rotten vegetable leaves can be effectively increased when the microbial agent is used for rapid rotting of the rotten vegetable leaves, and meanwhile the yield of next-stubble crops can be effectively increased.

The invention relates to the technical field of pulping, in particular to a bamboo reed raw material biological fermentation cellulose pulping method and application thereof. Bacillus licheniformis, bacillus subtilis, bacillus pumilus and bacillus cereus are compounded into a complex microbial inoculant, the fermented bamboo reed raw material is used for pulping, all strains are mutualistic and synergistic, meanwhile, a rich complex enzyme system is provided, the prepared paper is good in physical performance and high in quality, pulping is conducted through biological treatment, and the paper is environmentally friendly and environmentally friendly. The use of a large amount of chemical agents in the traditional pulping is avoided, and the discharge of wastewater in the traditional pulping process is reduced.

The present invention provides an essence containing chlorella extract and a preparation method thereof. The chlorella extract process comprises the following steps: raw material pulverization; enzymatic hydrolysis with cellulase and trypsin; primary fermentation using a mixture of Bacillus natto and Bacillus pumilus; addition of bamboo leaf extract, corn steep liquor, and sucrose, followed by inoculation with Rhodotorula glutinosus for secondary fermentation to obtain the chlorella extract. The mixed fermentation of multiple strains exhibits a synergistic effect, resulting in greater enrichment of natural products during the fermentation process compared to single-strain fermentation. Furthermore, the present invention provides an essence containing chlorella extract, glycerin, sodium hyaluronate, honey, and water, which exhibits significant moisturizing, antioxidant, and soothing effects.

The invention provides heat-resistant bacillus pumilus P2-7 and application thereof in disease prevention and control of prawn culture, and belongs to the technical field of bioengineering. The heat-resistant bacillus pumilus P2-7 provided by the invention is preserved in Guangdong Microbial Culture Collection Center on November 4, 2024, and the preservation number of the heat-resistant bacillus pumilus P2-7 is GDMCC NO. 65408. The strain is different from the traditional antibacterial bacillus in antagonism mode, cannot secrete metabolites with antibacterial activity, plays a role in a phagocytosis mode, can remarkably remove / inhibit pathogenic bacteria in water, can effectively prevent and control shewanella disease of prawns, and has a good application prospect. The composition also has a good effect of simultaneously preventing septicemia, acute hepatopancreas necrosis, white spot syndrome and infectious subcutaneous and hematopoietic necrosis in prawn culture production.

The invention discloses a strain NJSH56 for promoting plant growth and application thereof, the strain NJSH56 is identified as bacillus pumilus, the strain NJSH56 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation time is November 14, 2024, and the strain preservation number is CGMCC NO.32614. The strain NJSH56 can promote plant growth. The dendrobium officinale endophytic growth-promoting strain NJSH56 for promoting plant growth is obtained through separation and screening, growth of dendrobium officinale tissue culture seedlings can be effectively promoted, and the fresh weight, the rooting number and the leaf number are increased; greenhouse tests show that the number of new buds of dendrobium officinale plants in a strain NJSH56 treatment group reaches 4.67 and is obviously higher than that of a control group; meanwhile, the NJSH56 is used for treating the non-host plant Chinese cabbage, so that the growth of the overground part of the Chinese cabbage can be remarkably promoted, and the biomass accumulation of the Chinese cabbage is improved.

The invention relates to the technical field of calcium carbonate manufacturing, in particular to a preparation method of high-whiteness light calcium carbonate. Comprising the following steps: efficiently leaching the calcium element in the carbide slag; preparing a microbial crystal form control agent; and carrying out electric dispersion treatment and precipitation on calcium ions. The microbial crystal form control agent is prepared by regulating and controlling the mixing ratio of the bacillus pumilus fermentation liquor to the pseudomonas fluorescens fermentation liquor, and it is avoided that in the full mixing process of the microbial crystal form control agent and the calcium-containing filtrate, Ca < 2 + > is mineralized to form large calcium carbonate particles; stearic acid and sodium polyacrylate in the microbial crystal form control agent can enable particles to be more stable, provide ion adsorption capacity, optimize the crystal form of calcium carbonate and form vaterite type crystals with smaller crystal form, and bacillus pumilus can assist generation of small-particle-size calcium carbonate along with time, so that high-whiteness small-particle-size light calcium carbonate is obtained.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus pumilus, a microbial agent, a method and application. The invention provides a bacillus pumilus G15, and the preservation number of the bacillus pumilus G15 is CGMCC (China General Microbiological Culture Collection Center) No.32839. The bacillus pumilus G15 has a remarkable inhibition effect on pectobacterium variabilis FN20111, the invention further provides a fungicide, and potting control effect determination shows that the bacillus pumilus G15 has a high control effect on the pectobacterium variabilis FN20111.

When fed to animals, Bacillus licheniformis strain O42AH3 (deposit accession number DSM 32559), Bacillus subtilis strain O52YJ6 (deposit accession number DSM 32560), Bacillus amyloliquefaciens strain O52YYT (deposit accession number DSM 32561) and / or Bacillus pumilis O72NR7 (deposit accession number DSM 32563) are useful for preventing and / or treating Lawsonia infections and for improving myriad performance parameters, including, but not limited to, body weight gain, average daily gain and feed conversion rates.

The invention relates to the technical field of lubricating oil, and discloses degradable emulsified antirust lubricating oil and a preparation method thereof.The lubricating oil is prepared from, by weight, 10-20% of cobalt salt antirust component, 8-20% of emulsifier, 5-8% of fungi, 10-20% of lubricant, 0-1% of coupling agent, 0-0.2% of corrosion inhibitor, 3-6% of stabilizer and the balance base oil; the cobalt salt anti-rust component is a metal salt anti-rust component taking cobalt salt as a main component; the fungus is any one or a mixture of several of bacillus pumilus, bacillus subtilis and bacillus cereus; the preparation method comprises the following steps: adding the cobalt salt antirust component, the co-emulsifier, the fungi, the lubricant, the coupling agent, the corrosion inhibitor, the stabilizer and the base oil into a reaction kettle, heating to 45-50 DEG C, and uniformly stirring; and at the temperature of 45-50 DEG C, adding the main emulsifier while stirring until the system is uniform and transparent, thereby obtaining the anti-rust lubricating oil. The problems that existing antirust lubricating oil is large in smell, and water for cleaning metal workpieces pollutes the environment are solved.

The invention belongs to the technical field of microbial agents, and particularly relates to a microbial agent containing bacillus velezensis and application of the microbial agent to astragalus membranaceus. The invention discloses a microbial agent for astragalus membranaceus. The microbial agent is prepared by mixing bacillus velezensis powder, bacillus subtilis powder and bacillus pumilus powder according to the mass ratio of 1: (1-10): (1-5), the bacterium content of the bacillus velezensis powder, the bacillus subtilis powder and the bacillus pumilus powder is 0.2-0.5 billion cfu / g. The bacillus velezensis, the bacillus subtilis and the bacillus pumilus are fermented respectively, fermentation liquor is taken to be prepared into powder, and the powder is mixed according to a certain mass ratio to form the compound microbial agent, so that the compound microbial agent can improve the prevention and treatment effect on the radix astragali root knot nematode disease, is green and environment-friendly, can effectively overcome the defects of chemical agents, and has a good application prospect. Support is provided for biological prevention and control of the radix astragali root knot nematode disease, and meanwhile healthy growth of radix astragali plants can be guaranteed.

The invention provides a compound feed additive for relieving stress response of marine fishes, which is prepared by the following steps: mixing raw materials such as hermetia illucens protein and the like to prepare a premix, and embedding the premix by using a lac-glycyrrhizic acid system to prepare lac microcapsules; then modifying the fucoidin by adopting glycyrrhizin and gamma-linolenic acid, embedding rhodosporidium palustris and bacillus pumilus by using the fucoidin to prepare compound bacterium nano-particles, and finally mixing the lac microcapsules and the compound bacterium nano-particles in proportion. According to the invention, excessive secretion of cortisol of marine fish can be effectively inhibited, hermetia illucens protein provides rich nutrition and reduces muscle decomposition, and compound microorganisms are beneficial to maintaining intestinal microecological balance and improving the utilization rate of nutrition. The prepared compound feed additive can be directly splashed into a culture or transportation water body or added into a feed, so that the stress reaction of the marine fish is effectively relieved, the survival rate of the marine fish is increased, and powerful support is provided for culture of the marine fish.

The invention relates to the technical field of functional microorganism screening and application, and particularly provides a microecological preparation containing bacillus pumilus and application of the microecological preparation in aquaculture. The microecological preparation can significantly promote the growth of aquatic animals, improve the feed utilization rate, enhance the oxidation resistance and organism immunity of the aquatic animals, reduce the occurrence of diseases, reduce tissue loss, significantly improve the apparent digestibility of the aquatic animals, reduce environmental pollution caused by nitrogen and phosphorus emissions, and improve the economic benefits of the aquatic animals. The method can be widely applied to the fields of aquatic feed production.

The present disclosure relates generally to compositions comprising Bacillus pumilus having a positive impact on animal performance parameters, the use of Bacillus pumilus for preventing, reducing or controlling bacterial colonization or infection, a method of improving one or more animal performance parameters, and a kit. In particular, the present disclosure relates to Bacillus pumilus DSM 34300 and DSM 34299.

The invention discloses a zinc-selenium-rich sour soup fermentation strain and a preparation method thereof, and belongs to the technical field of microbial fermentation. The composite strain is composed of lactobacillus plantarum MJ-ZX01, saccharomyces cerevisiae MJ-ZX02 and bacillus pumilus MJ-ZX03 according to the weight ratio of 5: 3: 2, all of which are obtained by screening from the zinc-selenium environment of Majiang River in Guizhou and have efficient zinc-selenium enrichment and transformation capacity. The preparation method adopts a stepped fermentation process and comprises the following steps: carrying out anaerobic fermentation at 37 DEG C until the pH value is 4.2 in 0-24 hours under the leading of MJ-ZX01; in 24-72 hours, MJ-ZX02 and MJ-ZX03 cooperate with each other, anaerobic fermentation is performed at 30 DEG C until the pH value is 3.8, and the initial Zn content is controlled to be 50 mg / L, the initial Se content is controlled to be 10 mg / L, and the pressure of the fermentation tank is controlled to be-0.05 MPa. According to the technology, the zinc retention rate is greater than or equal to 85%, the selenium retention rate is greater than or equal to 80%, the flavor substance content is doubled, the fermentation period is shortened to 5 days, the technology is suitable for industrial production, and the nutritional value and market competitiveness of the sour soup can be remarkably improved.

The invention relates to the technical field of functional microorganism screening and application, and particularly provides bacillus pumilus with salt-tolerant and growth-promoting functions and application of the bacillus pumilus. The preservation number of the bacillus pumilus is CCTCC NO: M20231590, and the bacillus pumilus can effectively relieve crop salt damage, promote crop growth, increase crop yield, improve the harm of increasingly serious land salinization to agricultural planting and ensure the crop yield and quality.

According to the agricultural liquid fertilizer for tomato planting, bacillus licheniformis, bacillus amyloliquefaciens, bacillus mucilaginosus and bacillus pumilus are subjected to liquid fermentation and then mixed to prepare the novel agricultural liquid fertilizer for tomato planting. According to the present invention, the tomato growth promotion effect is significant, the prevention and control effects on tomato early blight, tomato black spot pathogen, tomato fusarium wilt, tomato gray mold and tomato anthracnose are significant, the tomato survival rate can be improved, the promotion effect is provided, the fruit quality is improved, the important significance is provided for tomato planting, and the economic benefit is significant. The liquid fertilizer can change the soil flora structure, increases the soil fertility and permeability, and plays an important role in soil improvement.

The invention belongs to the technical field of microorganisms and application, and particularly relates to bacillus pumilus K40, a culture method of the bacillus pumilus K40, a microbial agent containing the bacillus pumilus K40 and application of the bacillus pumilus K40. The invention provides a bacillus pumilus K40 with a preservation number of CCTCC NO: M 2025655, the bacillus pumilus K40 is preserved in China Center for Type Culture Collection on March 31, 2025, and the preservation address is No. 299 on eight road in Wuchang District, Wuhan City, Hubei Province. The strain K40 provided by the invention can inhibit the growth of pathogenic bacteria pseudomonas elongata of white mold of morchella esculenta, and the bacteriostasis rate is 56.06%; meanwhile, the strain can promote formation of bacterial cream, the yield of morchella esculenta is increased by 20% or above, and the occurrence rate of white mold is reduced by 85% or above. The bacillus pumilus K40 provided by the invention is of great significance to the cultivation industry of morchella esculenta.

The application relates to the technical field of tail-cabbage waste resource utilization, in particular to a microbial agent and application thereof in the field of tail-cabbage fast rot. The microbial agent comprises Bacillus pumilus and Bacillus subtilis; the Bacillus subtilis is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 33122; the Bacillus pumilus is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 33121. The application also provides application of the microbial agent in the field of tail-cabbage fast rot. The application widens the tail-cabbage fast rot treatment method, enriches the microbial species that can be used for tail-cabbage fast rot, and can effectively improve the tail-cabbage rotting rate and the yield of the next crop by using the microbial agent provided by the application to carry out tail-cabbage fast rot.

The invention belongs to the technical field of microorganisms and soil improvement, and particularly relates to saline-alkali tolerant bacillus pumilus and application thereof in saline-alkali soil improvement. The biochar, the humified agricultural and animal husbandry waste and the saline-alkali tolerant microorganisms are used for improving the saline-alkali soil, compared with a method for improving the saline-alkali soil by singly using the humified agricultural and animal husbandry waste or the biochar, the method has a better soil improvement effect, meanwhile, crops are planted in the improved saline-alkali soil, and the saline-alkali soil is improved. The growth of various crops including corn can also be promoted. Furthermore, the bacillus pumilus L13 which is obtained through self-separation and has saline-alkali tolerance and growth promotion effects is applied to the saline-alkali soil improvement method, and the saline-alkali soil improvement effect is better.

The present application relates to the technical field of calcium carbonate manufacturing, and particularly relates to a preparation method of high-whiteness light calcium carbonate. The method comprises the following steps: efficient leaching of calcium element in carbide slag; preparation of microbial crystal form control agent; and electric dispersion treatment and precipitation of calcium ions. The microbial crystal form control agent is prepared by adjusting the mixing ratio of Bacillus pumilus fermentation liquor and Pseudomonas fluorescens fermentation liquor, so that Ca 2+ The stearic acid and sodium polyacrylate in the microbial crystal form control agent can make the particles more stable and provide ion adsorption capacity after the larger calcium carbonate particles are mineralized, CO2 is introduced after temperature rising, and the crystal form of the calcium carbonate is optimized, so that the vaterite type crystallization with smaller crystal form is formed. The Bacillus pumilus can also assist in generating small-particle-size calcium carbonate over time, so that the high-whiteness small-particle-size light calcium carbonate is obtained.

The application provides a bacillus pumilus and application thereof, the bacillus pumilus is preserved in Guangdong Microbial Culture Collection Center on July 24, 2025, and the preservation number is GDMCC No: 66749;The 16S rDNA of the bacillus pumilus is as shown in SEQ ID NO.1;The application of the bacillus pumilus is to prepare microbial preparation comprising the bacillus pumilus;The bacillus pumilus has good phosphorus dissolving capacity and IAA production capacity, can enhance the resistance of plants and promote the growth of plants.