40 results about "Citrus canker" patented technology

This invention discloses a class of thiadiazole-benzohexacyclic compounds, their preparation methods, and applications. The structural formula is shown in Formula I. This invention introduces disulfide bond fragments into the thiadiazole-benzohexacyclic skeleton to obtain a novel class of thiadiazole-benzohexacyclic compounds. These compounds have a novel structure, a simple preparation method, readily available raw materials, and are unlikely to produce harmful byproducts. The compounds exhibit inhibitory effects against various plant pathogenic bacteria, particularly against *Citrus canker*, *Ralstonia solanacearum*, and *Bacterial angular leaf spot* of cucumber, demonstrating significant inhibitory effects. They can be used as agricultural bactericides and have great application value.

The invention relates to the field of pesticide preparations, and discloses a sterilization composition containing tetramycin and Saisentong as well as a preparation method and application of the sterilization composition. Tetramycin and Saisentong serve as active components of the composition, and a dual-structure composite lignin derivative system serves as a functional carrier; the system is composed of high-molecular-weight modified lignosulfonate and specific lignin phenol fragments with low polymerization degree. The high-molecular-weight component forms a physical barrier on the surfaces of active component particles, so that the storage stability and the application safety of the preparation are improved; the low-polymerization-degree component can be degraded by an extracellular enzyme secreted by citrus canker pathogen, so that the targeted release of the active component at the focus is realized. The composition disclosed by the invention effectively reduces the decomposition rate of the active components and the phytotoxicity risk to crops, and meanwhile, the field control effect on the citrus canker is remarkably improved. The composition provides an efficient and safe new choice with a targeting effect for chemical prevention and treatment of citrus canker.

The application discloses a pesticide composition containing zhongshengmycin and kanamycin and application thereof, and belongs to the technical field of pesticides. Effective components of the pesticide composition are zhongshengmycin and kanamycin. The mass ratio of the kanamycin to the zhongshengmycin is 1:1-9. The two effective components of the application have different action sites for killing pathogenic bacteria, and can effectively alleviate the generation of drug resistance of the pathogenic bacteria. After the zhongshengmycin and the kanamycin are compounded, synergistic effect is obvious, the control effect of the pesticide on bacterial diseases, such as citrus canker, is improved, the pesticide dosage can be reduced, and agricultural residues are reduced. Meanwhile, the prepared soluble granules do not use any organic solvent, are safe to crops, and have no residues and pollution to the environment.

This invention relates to the field of medicinal chemistry, specifically to a capsaicin-like compound containing isopropanolamine, its preparation method, and its application. This type of compound has a structure as shown in general formula (I). The invention uses capsaicin and isopropanolamine as pharmacophores, connecting the two pharmacophores with different bridging chains to synthesize a series of capsaicin-like derivatives containing isopropanolamine. These compounds exhibit excellent inhibitory effects on plant pathogenic bacteria such as rice bacterial blight, citrus canker, rice bacterial leaf streak, and kiwifruit canker; and plant pathogenic fungi such as cucumber gray mold, pepper wilt, rapeseed sclerotinia rot, eggplant verticillium wilt, wheat scab, potato late blight, blueberry root rot, grape coccidioidomyces, dragon fruit anthracnose, and rice sheath blight, providing an important scientific basis for the research and development of new pesticides.

This invention discloses the antimicrobial peptide PJX13 and its application in the control of citrus canker. The sequence of the antimicrobial peptide PJX13 is AcKKFWKLRRWVKWIGNH2. It can effectively inhibit and kill *Xanthomonas citrus* by disrupting the cell membrane integrity of pathogens, leading to leakage of intracellular substances. It effectively inhibits the expansion of lesions on both detached citrus leaves and living plants, and can be formulated into an antimicrobial product against *Xanthomonas citrus* for the control of citrus canker. Furthermore, the antimicrobial peptide PJX13 can also inhibit *Escherichia coli*, *Staphylococcus aureus*, and *Bacillus oryzae*, and can be used to prepare broad-spectrum antimicrobial products. This invention is beneficial for the control of citrus canker, and has advantages such as being environmentally friendly and unlikely to induce drug resistance.

The application provides a Yunnan golden camellia root endophytic fungus and application thereof, and relates to the technical field of crop disease prevention and treatment. The Yunnan golden camellia root endophytic fungus is Lecanicillium attenuatum JHC098, which is preserved in the China Center for Type Culture Collection and has a preservation number of CCTCC NO: M 2025749. The liquid fermentation broth extract of the fungus can be applied to crop disease prevention and treatment. The application overcomes the defects of the prior art. The liquid fermentation broth extract of the Yunnan golden camellia root endophytic fungus Lecanicillium attenuatum JHC098 can effectively inhibit the growth of citrus canker and tobacco bacterial wilt pathogenic bacteria, so that the purpose of biological prevention is achieved.

The invention discloses a tricyclic compound containing an indole structure as well as a composition and agricultural application of the tricyclic compound, and particularly relates to a tricyclic compound of optionally substituted or unsubstituted C4-7-membered saturated and unsaturated alkyl and alkyl impurity groups of indole and a composition and pesticide application of the tricyclic compound of optionally substituted or unsubstituted C4-7-membered saturated and unsaturated alkyl and alkyl impurity groups of indole. The compound provided by the invention has good inhibitory activity on plant pathogenic bacteria and fungi, particularly, the compound A-10 shows broad-spectrum and excellent inhibitory activity on test strains, and EC50 of the compound A-10 on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and Xanthomonas syringae pv. Kiwiis is 8.55 mu g / mL, 2.70 mu g / mL and 9.89 mu g / mL respectively.

The invention discloses application of any compound of 8-substituted berberine derivatives in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative has a significant effect on four plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri, pseudomonas solanacearum and Ralstonia solanacearum, and four plant pathogenic fungi such as rhizoctonia solanacearum, sclerotinia sclerotiorum, botrytis cinerea and fusarium graminearum. The compound shows certain inhibitory activity on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes, and has a good inhibitory effect on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel plant disease resistant medicine.

This divisional application discloses the use of a 2,8-bis(trifluoromethyl)-4-hydroxyquinoline derivative in the control or treatment of agricultural pathogenic bacteria. The structural formula of the 2,8-bis(trifluoromethyl)-4-hydroxyquinoline derivative is as follows: Bioactivity tests show that this derivative exhibits excellent inhibitory activity against two plant bacteria: rice bacterial blight pathogen and citrus canker pathogen. Compound Q-29 shows an MIC90 value of 1.56 μg / mL against both rice bacterial blight pathogen and citrus canker pathogen, significantly outperforming the control drugs thiabendazole and thiabendazole copper. The compounds involved in this invention are easy to prepare, use inexpensive and readily available raw materials, and are expected to be developed into a novel agricultural antibacterial agent.

The invention belongs to the technical field of molecular biology, and particularly discloses a method for improving citrus canker resistance by using a CsENPP1 gene, the coding protein of the CsENPP1 gene is citrus nucleotide pyrophosphatase / phosphodiesterase 1, the coding sequence of the CsENPP1 is a nucleotide sequence shown as SEQ ID No.1, and the citrus canker resistance is improved based on overexpression of the CsENPP1 gene in citrus cells. The citrus nucleotide pyrophosphatase / phosphodiesterase 1 coding gene is integrated into citrus through an expression vector, the canker attack degree of the obtained transgenic material can be reduced to 48.9% of that of the existing citrus to the maximum extent, the canker attack degree can be remarkably reduced, the scab area is reduced, and the yield of the citrus is improved. The technology provided by the invention is a bioengineering technology which has potential to improve the citrus canker resistance, and has great value for citrus canker resistance molecular breeding.

The invention relates to an ether-containing pleuromutilin derivative as well as a preparation method and application thereof. The structural formula of the pleuromutilin derivative is shown as a formula Y. An antibacterial activity test on the pleuromutilin derivative containing ethers proves that the pleuromutilin derivative containing ethers has relatively good antibacterial activity on Xanthomonas oryzae pv. Oryzae, Pseudomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citri. And the preparation method is simple, and the production cost is low.

Disclosed herein are methods and compositions for use in controlling citrus greening, citrus canker, or fire blight, wherein the compositions comprise one or more antimicrobial peptides, or one or more polynucleotides encoding for the one or more antimicrobial peptides.

This invention discloses the application of the natural product santalol as an agricultural fungicide in plant disease control, belonging to the field of plant disease prevention and control. It shows good inhibitory effects against bacterial diseases such as rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwifruit canker, tobacco bacterial wilt, cucumber bacterial angular leaf spot, and potato bacterial wilt. It also shows good inhibitory effects against viral diseases such as tobacco mosaic virus; and against fungal diseases such as rice rhizome blight, wheat scab, pepper wilt, *Botrytis cinerea*, or *Colletotrichum sorghum*. It can be used in the preparation of plant fungicides. Santalol is a natural plant extract and is environmentally friendly. This invention provides a new use for the natural product santalol, enabling its application in the agricultural field for plant disease control and providing a new avenue for the research and development of new pesticides.

The invention relates to the field of pesticides, and provides an agricultural composition as well as a preparation method and application thereof. The invention provides application of an agricultural composition in preparation of a pesticide bactericide and / or a plant growth regulator. The active ingredients of the agricultural composition comprise an aloe extract and rhamnolipid. According to the present invention, the aloe extract and the rhamnolipid are adopted as the active components of the agricultural composition, and through the specific ratio, the two active components can achieve the synergistic interaction, and particularly have the excellent prevention and control effect on bacterial infected plant diseases, such as the significant effect on the prevention and control of rice bacterial streak, citrus canker and cucumber bacterial angular leaf spot, meanwhile, the growth of new shoots and new leaves of the plants can be promoted, and the method has excellent commercial value.

The invention discloses pantoea for efficiently preventing and treating citrus canker and application of the pantoea. The pantoea is named as Pantoea cypripedii Q210, the preservation number is CGMCC NO.36920, the preservation date is December 8, 2025, and the preservation site is the China General Microbiological Culture Collection Center. The bacterial strain is separated and screened from soil of a Gannan navel orange orchard, and it is found that the bacterial strain has the capacity of inhibiting growth of citrus canker pathogen Xcc, has an obvious prevention and treatment effect on citrus canker and can provide a new biological prevention and treatment means for comprehensive prevention and treatment of citrus canker.

The application discloses a method, reagent and application for identifying or tracing citrus canker bacteria. The method comprises the following steps: obtaining seven gene sequences of the to-be-tested citrus canker bacteria, performing multi-locus sequence typing analysis on the seven gene sequences, determining alleles and numbering, concatenating the allele numbers of the seven genes to serve as a sequence type, comparing the sequence type with a reference strain sequence type, and obtaining tracing information of the to-be-tested strain; the reference strain is a citrus canker bacteria with known tracing information; the seven genes are XAC_RS07980, XAC_RS21550, copB, egl, fliL, hrpA and pliY. The seven housekeeping genes suitable for multi-locus sequence typing of the citrus canker bacteria are used for tracing detection, different geographical source strains can be distinguished at high resolution, and the method has important significance for strain regional research, evolution, transmission, disease prevalence and prevention and treatment. The method can be used for identifying and tracing the citrus canker bacteria.

The application discloses a method for improving citrus canker resistance by interfering with CsRHY1A, and relates to the field of agricultural biological genes. The RNAi fragment of the CsRHY1A coding gene of citrus is cloned, the interference vector is integrated into the citrus, the transcription level of CsRHY1A is reduced, the resistance of the citrus to canker is significantly improved, the phenotype of the transgenic plant is not affected, the canker lesion area of the obtained transgenic plant can be reduced to 85% of that of the existing citrus at the most, the disease index can be reduced to 82% of that of the existing citrus at the most, the disease degree of canker can be significantly reduced, and the phenotype of the citrus is not affected, so that it is concluded that the interference of CsRHY1A can greatly improve the resistance of the citrus to canker; compared with the gene editing technology for silencing the citrus gene, the RNAi technology is more stable, the probability of obtaining a gene-silenced plant is greater, and the RNAi technology is suitable for the high-heterozygosity species of the citrus.

The invention discloses an application of any compound of an Oxoapophine alkaloid derivative in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative shows certain inhibitory activity on two plant pathogenic bacteria such as rice bacterial blight and citrus canker pathogen, and five plant pathogenic fungi such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, magnaporthe grisea and wheat stem rot; the compound has a good inhibition effect on citrus canker pathogen and sclerotinia sclerotiorum. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel agricultural antibacterial agent.

The invention discloses a quinoline derivative containing an alkenyl structure as well as a preparation method and application of the quinoline derivative, the compound has a structure as shown in a general formula (I), and the compound has a specific effect on plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and Xanthomonas syringae pv. Kiwifruit; the plant pathogenic fungi such as rhizoctonia solani, botrytis cinerea, eggplant verticillium wilt and the like can be inhibited. All the compounds show excellent inhibitory activity on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citri, the EC50 of the compounds is 0.024-6.524 [mu] g / mL and 0.074-8.718 [mu] g / mL respectively, part of the compounds still have good inhibitory activity on Xanthomonas citri pv. Citri, the EC50 of the compounds is 2.036-10.776 [mu] g / mL, and the compounds can be used for preparing pesticides for resisting plant pathogenic bacteria.

The invention discloses an application of N-phenylmaleimide as a synergist of a copper-based bactericide, and finds for the first time that the N-phenylmaleimide and the copper-based bactericide are mixed for use, so that the dosage of the copper-based bactericide can be reduced, and meanwhile, good bactericidal activity can be ensured; and verification is carried out on mango bacterial angular leaf spot bacteria and citrus canker bacteria, and the N-phenylmaleimide (NPM) shows a remarkable synergistic effect when being mixed with the copper-based bactericide. The use amount of the copper-based bactericide in unit area is reduced, the accumulation risk of heavy metals in soil is reduced, resistance evolution of pathogenic bacteria to copper is delayed, and the method has important significance for solving the increasingly prominent drug resistance problem of pathogenic bacteria.

The application discloses a kind of active substances for crop protection and its composition and preparation method and application, the substance general formula as follows: Wherein: R1 It is hydrogen atom, halogen, cyano, methyl, methoxy, nitro, trifluoromethyl or the disubstituted of arbitrary combination of above halogen;R2 It is hydrogen atom, halogen or nitro substitution.This application derivative shows better inhibitory effect to rice bacterial leaf spot, rice bacterial leaf blight, kiwi bacterial canker, citrus canker.The inhibitory activity of compound II-18 to kiwi bacterial canker bacteria is best.Plant pathogenic fungi (sclerotinia sclerotiorum, phomopsis viticola, botryosphaeria dothidea and fusarium graminearum) inhibitory activity test shows that compound II-10 shows better inhibitory activity to fusarium graminearum.In addition, part of the derivatives of the application has significant contact toxicity to bactrocera dorsalis (Hendel).The composition of compound II-5 and methyl eugenol shows synergistic trapping effect in the process of preventing and treating the main pest of kiwi, bactrocera dorsalis (Hendel).

This invention discloses a *Pseudomonas* strain that controls citrus canker and promotes plant growth, along with its applications. *Pseudomonas* sp. CR1201 was deposited on January 17, 2024, at the Guangdong Provincial Microbial Culture Collection Center (GDMCC), with accession number GDMCC No.: 64290. The *Pseudomonas* strain disclosed in this invention is a new species in this genus, capable of effectively antagonizing *Xcc*, the causal agent of citrus canker, and promoting plant growth by producing siderophores and the plant growth hormone IAA. Therefore, *Pseudomonas* CR1201 disclosed in this invention can be used to prepare microbial preparations for controlling citrus canker, and also has significant application potential in microbial agents and biofertilizers for promoting plant growth and development.

The present application relates to the use of 3,3-dimethylsulfonyl-2-cyanoacrylic acid derivatives. The compound has a structure as shown in general formula (I): such compounds have good inhibitory effect on pathogenic bacteria such as rice bacterial leaf blight, citrus canker, etc. At the same time, the compounds have good growth-promoting effect on corn and tomato in plant growth regulation test.

The invention discloses application of a carbazole ring-containing natural product as an agricultural bactericide in plant diseases. The carbazole ring natural product has a good inhibition effect on plant bacterial diseases such as rice bacterial blight, citrus canker, tobacco bacterial wilt, kiwifruit canker or rice bacterial streak, and the ellipticine can be used as an innovative medicament for resisting rice bacterial blight, and the medicament shows a remarkable sterilization effect; aiming at plant fungal diseases such as cucumber gray mold, pepper fusarium wilt, sclerotinia rot of colza, wheat scab, potato late blight, blueberry root rot, botryosphaeria dothidea, pitaya anthracnose, rice sheath blight disease, fusarium SP or colletotrichum graminearum and the like, the invention provides a new application of a carbazole ring-containing natural product which can be used for preparing a plant fungicide. The composition can be used for preventing and treating plant diseases in the agricultural field.

This application relates to a strain of *Pseudomonas lundense* and its applications. The present invention isolates a strain of *Pseudomonas lundense* from the root soil of rice plants in a rice cultivation base. This strain is effective against rice basal rot pathogens (…). Dickeya oryzae ), potato soft rot fungus ( Dickeya dadantii ), and Taro soft rot fungus ( Dickeya fangzhongdai It has a good control effect on sugarcane smut ( ) Sporisorium scitamineum ), rice blast fungus ( Magnaporthe oryzae ), Neodatura spp. Neoscytalidium dimidiatum ), neatly arranged small sclerotia ( Sclerotium rolfsii ), Lithocarpus ( Calonectria ), Black spores ( Nigrospora lacticoloni a) Ralstonia solanacearum ( Ralstonia solanacearum Xanthomonas aeruginosa (), Xanthomonas campestris pv. Campestris ) and citrus canker pathogen ( Xanthomonas citri subsp. citri It also has a good control effect. Therefore, it can be widely used in the control of plant diseases, including rice basal rot.

The application belongs to the technical field of molecular biology, and specifically discloses a method for improving the citrus canker resistance by using a CsENPP1 gene, wherein the coding protein of the CsENPP1 gene is a citrus nucleotide pyrophosphatase / phosphodiesterase 1, the CsENPP1 coding sequence is a nucleotide sequence shown in SEQ ID No. 1, and the citrus canker resistance is improved based on the overexpression of the CsENPP1 gene in citrus cells. The application integrates a citrus nucleotide pyrophosphatase / phosphodiesterase 1 coding gene into citrus through an expression vector, and the disease incidence of the obtained transgenic material can be reduced to 48.9% of that of the existing citrus at most, so that the disease incidence of the canker can be significantly reduced, and the lesion area can be reduced. The application is a biological engineering technology which has the potential to improve the citrus canker resistance, and has great value for the molecular breeding of the citrus canker resistance.

The application discloses a method for enhancing the resistance of late-kumquat to citrus canker by a citrus CsATL66 gene, and relates to the technical field of plant genetic engineering, and the method comprises the following steps: cloning a VIGS fragment of the citrus CsATL66 gene and constructing a VIGS expression vector of a CsATL66 coding sequence, and then transforming the citrus by using an Agrobacterium tumefaciens mediation method, so that the transcription level of the CsATL66 in the transgenic citrus is successfully reduced, and a VIGS plant in which the citrus CsATL66 gene is silenced is obtained. The citrus CsATL66 gene is cloned for the first time, the transcription level of the citrus CsATL66 is reduced by using VIGS silencing, and the function of the CsATL66 in the resistance and susceptibility of the citrus to the citrus canker is verified. The application has great application value for the breeding of the citrus to resist the citrus canker, lays a foundation for the genetic breeding of the citrus to resist the citrus canker, and greatly promotes the development and application of the gene engineering of the citrus to resist the citrus canker.

The invention relates to an agrobacterium bacterium and an application thereof in prevention and treatment of citrus canker, the agrobacterium bacterium is a 11B7 strain, the taxonomic Latin name of the agrobacterium bacterium is Agrobacterium Fabrum, the agrobacterium bacterium is preserved in the China Center for Type Culture Collection on November 20, 2024, and the preservation number is CCTCC NO: M 20242599. The Agrobacterium 11B7 strain provided by the invention can effectively inhibit the proliferation of Xcc in citrus, significantly alleviate citrus canker diseases, and can effectively colonize on the surfaces of citrus leaves and fruits to further enhance the prevention and treatment effect of citrus canker, and when in use, the microbial agent is prepared and then sprayed on the surfaces of plants, and the method is simple and efficient, and has great application value.

The application discloses a quinina derivative for preventing and treating agricultural diseases, a preparation method and application thereof, and relates to a compound with a structural formula as shown in formula I, or a pharmaceutically acceptable salt, a solvate and a tautomer thereof. It is found that the quinina derivative has excellent inhibitory effect on Xanthomonas oryzae and Xanthomonas citri, has great development value in treating plant pathogenic bacteria, and provides more choices for preventing and treating agricultural diseases.