85 results about "Citrus canker" patented technology

The invention relates to the technical field of agricultural biological genes, and discloses a method for improving citrus canker resistance based on a CsKCS2 gene, and the citrus canker resistance of citrus plants is improved by adjusting the expression level of the CsKCS2 gene in citrus plants. The method specifically comprises the following steps: (1) cloning a citrus CsKCS2 gene sequence; (2) constructing an overexpression vector; and (3) transforming the overexpression vector into citrus to obtain a transgenic material with an up-regulated CsKCS2 gene expression level. By cloning the coding sequence of the citrus CsKCS2 gene, constructing an overexpression vector and then transforming the citrus, the canker attack degree of the obtained CsKCS2 overexpression transgenic plant can be reduced to 73.3% of that of the existing citrus to the maximum extent, the canker attack degree can be effectively and remarkably reduced, the scab area is reduced, the phenotype of the transgenic plant is not influenced, and the application prospect is broad. The gene has a great application value for canker-resistant breeding of citrus, and can be used as a candidate gene for canker-resistant breeding with a plurality of canker-resistant and susceptible genes.

The invention discloses a citrus growth promoting fertilizer and a fertilization method thereof, and relates to the technical field of chemical fertilizer production, the citrus growth promoting fertilizer comprises 40-60 parts of macroelements, 15-25 parts of medium elements, 8-12 parts of trace elements, 10-18 parts of an organic active agent, and 5-10 parts of a slow release agent. Therefore, firstly, the nano calcium carbonate is used for replacing a traditional calcium source, and a polylactic acid-fucoidan slow-release membrane technology is combined, so that the dissolved state keeping time of calcium ions in the soil is prolonged by 30-50%, the calcium absorption rate is increased to 35% or above from the average industrial 20-30%, and the fruit cracking rate of citrus is effectively reduced; the bacillus subtilis and the seaweed extract in the organic active agent have a synergistic effect, through biological antagonism and induced resistance, the canker incidence rate is reduced to 1 / 4 of that of a control group, meanwhile, the soil microenvironment is improved through humic acid, and the root system health degree is improved by 40%.

The present invention discloses the application of coumarin-3-carboxylic acid in preventing and treating plant bacterial diseases. The coumarin-3-carboxylic acid provided by the present invention has a significant inhibitory effect on plant bacterial diseases. The plant bacterial diseases are oryzae bacterial leaf blight, oryzae bacterial leaf streak, citrus canker, cantaloupe bacterial fruit spot, tomato bacterial wilt, strawberry bacterial angular leaf spot, cabbage black rot, cabbage soft rot, potato soft rot, mango bacterial angular leaf spot, cassava bacterial wilt, cucumber bacterial angular leaf spot, kiwifruit canker and banana bacterial soft rot. The coumarin-3-carboxylic acid provided by the present invention has protective and therapeutic effects on melon bacterial fruit spot.

This invention discloses a class of thiadiazole-benzohexacyclic compounds, their preparation methods, and applications. The structural formula is shown in Formula I. This invention introduces disulfide bond fragments into the thiadiazole-benzohexacyclic skeleton to obtain a novel class of thiadiazole-benzohexacyclic compounds. These compounds have a novel structure, a simple preparation method, readily available raw materials, and are unlikely to produce harmful byproducts. The compounds exhibit inhibitory effects against various plant pathogenic bacteria, particularly against *Citrus canker*, *Ralstonia solanacearum*, and *Bacterial angular leaf spot* of cucumber, demonstrating significant inhibitory effects. They can be used as agricultural bactericides and have great application value.

The invention relates to the field of pesticide preparations, and discloses a sterilization composition containing tetramycin and Saisentong as well as a preparation method and application of the sterilization composition. Tetramycin and Saisentong serve as active components of the composition, and a dual-structure composite lignin derivative system serves as a functional carrier; the system is composed of high-molecular-weight modified lignosulfonate and specific lignin phenol fragments with low polymerization degree. The high-molecular-weight component forms a physical barrier on the surfaces of active component particles, so that the storage stability and the application safety of the preparation are improved; the low-polymerization-degree component can be degraded by an extracellular enzyme secreted by citrus canker pathogen, so that the targeted release of the active component at the focus is realized. The composition disclosed by the invention effectively reduces the decomposition rate of the active components and the phytotoxicity risk to crops, and meanwhile, the field control effect on the citrus canker is remarkably improved. The composition provides an efficient and safe new choice with a targeting effect for chemical prevention and treatment of citrus canker.

The application discloses a pesticide composition containing zhongshengmycin and kanamycin and application thereof, and belongs to the technical field of pesticides. Effective components of the pesticide composition are zhongshengmycin and kanamycin. The mass ratio of the kanamycin to the zhongshengmycin is 1:1-9. The two effective components of the application have different action sites for killing pathogenic bacteria, and can effectively alleviate the generation of drug resistance of the pathogenic bacteria. After the zhongshengmycin and the kanamycin are compounded, synergistic effect is obvious, the control effect of the pesticide on bacterial diseases, such as citrus canker, is improved, the pesticide dosage can be reduced, and agricultural residues are reduced. Meanwhile, the prepared soluble granules do not use any organic solvent, are safe to crops, and have no residues and pollution to the environment.

The invention discloses bacillus amyloliquefaciens capable of resisting plant pathogenic bacteria and application of the bacillus amyloliquefaciens, and belongs to the technical field of microorganisms, the bacillus amyloliquefaciens is classified and named as bacillus amyloliquefaciens KT-7, the bacillus amyloliquefaciens KT-7 is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on June 9, 2020, and the address is No.66, Courtyard No.100, Xianlie Middle Road, Guangzhou City, Guangdong Province; the preservation number of the strain is GDMCC No: 61051. The bacillus amyloliquefaciens provided by the invention has a growth inhibition effect on plant pathogenic bacteria, and particularly has a good control effect on bacterial diseases such as citrus canker and tomato bacterial wilt.

The invention relates to the technical field of microbial compositions, in particular to a microbial composition for preventing and treating citrus canker and application of the microbial composition. According to the microbial composition provided by the invention, the pseudomonas Qingdao microbial agent, sodium lignin sulfonate, trehalose, glutathione, ascorbic acid, lecithin and trace elements (Zn, B and Mn) are taken as main raw materials, and the effects of efficiently inhibiting bacteria, remarkably inducing and improving the activity of key defensive enzymes of leaves, promoting synthesis and accumulation of disease-resistant substances and improving the disease resistance of the leaves are achieved through the matching effect of all the components. The diversity and richness of citrus phyllospheric bacterial communities are improved, and the purpose of constructing a stable community dominated by beneficial bacteria is achieved. In other words, the basic stress resistance of the plants can be enhanced, and meanwhile, the growth and metabolism activity of beneficial bacteria is also promoted, so that effective, eco-friendly and sustainable prevention and control of the citrus canker are achieved.

The invention belongs to the technical field of prevention and control of plant bacterial diseases, and particularly relates to an amide derivative containing benzoxazole sulfone and a preparation method and application thereof.The amide compound containing benzoxazole sulfone has good antibacterial activity on plant pathogenic bacteria of rice bacterial leaf blight, rice bacterial streak and citrus canker, and can be used for preventing and controlling bacterial diseases of plants such as rice bacterial leaf blight, rice bacterial streak and citrus canker. The method has the advantages of simple preparation process, excellent reaction yield and low raw material cost; the structural formula of the derivative is shown as I,

The present application relates to the technical field of pharmaceutical chemistry, and particularly relates to application of tryptophanone in prevention and treatment of plant bacteria and part of plant fungi. The present application discloses that natural product tryptophanone can effectively prevent and treat plant bacterial and part of plant fungal diseases. The application of tryptophanone as an agricultural bactericide in plant bacterial diseases has excellent inhibitory effect on rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwi canker, tobacco bacterial wilt, cucumber bacterial angular spot, potato bacterial wilt, soybean bacterial spot, tomato canker and the like. The tryptophanone also shows good inhibitory activity on plant pathogenic fungi such as grapevine Eutypa lata, tobacco alternaria alternata, eggplant verticillium wilt, pepper fusarium wilt, colletotrichum gloeosporioides, tea tree colletotrichum gloeosporioides and sorghum zylosporium.

This invention relates to the field of medicinal chemistry, specifically to a capsaicin-like compound containing isopropanolamine, its preparation method, and its application. This type of compound has a structure as shown in general formula (I). The invention uses capsaicin and isopropanolamine as pharmacophores, connecting the two pharmacophores with different bridging chains to synthesize a series of capsaicin-like derivatives containing isopropanolamine. These compounds exhibit excellent inhibitory effects on plant pathogenic bacteria such as rice bacterial blight, citrus canker, rice bacterial leaf streak, and kiwifruit canker; and plant pathogenic fungi such as cucumber gray mold, pepper wilt, rapeseed sclerotinia rot, eggplant verticillium wilt, wheat scab, potato late blight, blueberry root rot, grape coccidioidomyces, dragon fruit anthracnose, and rice sheath blight, providing an important scientific basis for the research and development of new pesticides.

This invention discloses the antimicrobial peptide PJX13 and its application in the control of citrus canker. The sequence of the antimicrobial peptide PJX13 is AcKKFWKLRRWVKWIGNH2. It can effectively inhibit and kill *Xanthomonas citrus* by disrupting the cell membrane integrity of pathogens, leading to leakage of intracellular substances. It effectively inhibits the expansion of lesions on both detached citrus leaves and living plants, and can be formulated into an antimicrobial product against *Xanthomonas citrus* for the control of citrus canker. Furthermore, the antimicrobial peptide PJX13 can also inhibit *Escherichia coli*, *Staphylococcus aureus*, and *Bacillus oryzae*, and can be used to prepare broad-spectrum antimicrobial products. This invention is beneficial for the control of citrus canker, and has advantages such as being environmentally friendly and unlikely to induce drug resistance.

The invention discloses application of phenylpyruvic acid in prevention and control of citrus canker, and belongs to the field of treatment of agricultural canker. The invention discloses a new application of phenylpyruvic acid in treating and / or preventing citrus canker, and the effective dosage of phenylpyruvic acid is 0.01-1.5 M. The phenylpyruvic acid is externally applied, so that the scab area of the citrus canker can be reduced to a great extent, and the attack degree of the canker is reduced. In addition, the content of phenylpyruvic acid in citrus can be increased through multiple technologies, and the phenylpyruvic acid can be used for canker-resistant molecular breeding.

The application provides a method for improving the resistance of citrus to canker disease based on gene interference, which comprises the following steps: introducing a long-chain fatty alcohol oxidase gene in a citrus plant into an interference vector as an interference sequence, and integrating the interference sequence into the citrus genome by means of agrobacterium-mediated genetic transformation, so as to effectively improve the resistance of the citrus to canker disease. CsFAO3 The part of the coding sequence of the gene, i.e., the sequence as shown in SEQ ID NO: 3, is used as an interference sequence, introduced into an interference vector, and integrated into the citrus genome by means of agrobacterium-mediated genetic transformation, so as to effectively improve the resistance of the citrus to canker disease. The method can directly inhibit most of the pathogenic bacteria causing the canker disease of the citrus, the gene to be interfered with can greatly improve the resistance of the citrus to the canker disease while reducing the adverse effects on the known quality and performance of the citrus to the greatest extent, a new way for the breeding of the citrus resistant to the canker disease is opened up, and the development of the breeding of the citrus resistant to the canker disease is promoted.

The application provides a Yunnan golden camellia root endophytic fungus and application thereof, and relates to the technical field of crop disease prevention and treatment. The Yunnan golden camellia root endophytic fungus is Lecanicillium attenuatum JHC098, which is preserved in the China Center for Type Culture Collection and has a preservation number of CCTCC NO: M 2025749. The liquid fermentation broth extract of the fungus can be applied to crop disease prevention and treatment. The application overcomes the defects of the prior art. The liquid fermentation broth extract of the Yunnan golden camellia root endophytic fungus Lecanicillium attenuatum JHC098 can effectively inhibit the growth of citrus canker and tobacco bacterial wilt pathogenic bacteria, so that the purpose of biological prevention is achieved.

This invention discloses a method and application for enhancing citrus canker resistance using the CsMBL1 gene, relating to the field of agricultural biotechnology. The method for enhancing citrus canker resistance using the CsMBL1 gene includes the following steps: (1) cloning the citrus CsMBL1 coding sequence, as shown in SEQ ID No. 1; (2) constructing a CsMBL1 overexpression vector; (3) transforming citrus with the CsMBL1 overexpression vector, and after cultivation, obtaining transgenic plants with enhanced canker resistance. This invention effectively enhances citrus resistance to canker by integrating a citrus globular mannose-specific lectin binding gene into citrus through an expression vector, which has significant application value for citrus canker resistance breeding.

The invention provides a carpet grass xanthomonas phage, a compound phage preparation and application of the carpet grass xanthomonas phage and the compound phage preparation. The carpet grass xanthomonas phage is carpet grass xanthomonas phage XAC17P1, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20241062. The compound bacteriophage preparation provided by the invention has higher sterilization efficiency and better application stability, belongs to a green and efficient plant disease biological prevention and control means, and can effectively prevent and treat citrus canker.

The invention discloses a tricyclic compound containing an indole structure as well as a composition and agricultural application of the tricyclic compound, and particularly relates to a tricyclic compound of optionally substituted or unsubstituted C4-7-membered saturated and unsaturated alkyl and alkyl impurity groups of indole and a composition and pesticide application of the tricyclic compound of optionally substituted or unsubstituted C4-7-membered saturated and unsaturated alkyl and alkyl impurity groups of indole. The compound provided by the invention has good inhibitory activity on plant pathogenic bacteria and fungi, particularly, the compound A-10 shows broad-spectrum and excellent inhibitory activity on test strains, and EC50 of the compound A-10 on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and Xanthomonas syringae pv. Kiwiis is 8.55 mu g / mL, 2.70 mu g / mL and 9.89 mu g / mL respectively.

The invention discloses application of any compound of 8-substituted berberine derivatives in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative has a significant effect on four plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri, pseudomonas solanacearum and Ralstonia solanacearum, and four plant pathogenic fungi such as rhizoctonia solanacearum, sclerotinia sclerotiorum, botrytis cinerea and fusarium graminearum. The compound shows certain inhibitory activity on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes, and has a good inhibitory effect on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel plant disease resistant medicine.

This divisional application discloses the use of a 2,8-bis(trifluoromethyl)-4-hydroxyquinoline derivative in the control or treatment of agricultural pathogenic bacteria. The structural formula of the 2,8-bis(trifluoromethyl)-4-hydroxyquinoline derivative is as follows: Bioactivity tests show that this derivative exhibits excellent inhibitory activity against two plant bacteria: rice bacterial blight pathogen and citrus canker pathogen. Compound Q-29 shows an MIC90 value of 1.56 μg / mL against both rice bacterial blight pathogen and citrus canker pathogen, significantly outperforming the control drugs thiabendazole and thiabendazole copper. The compounds involved in this invention are easy to prepare, use inexpensive and readily available raw materials, and are expected to be developed into a novel agricultural antibacterial agent.

The invention belongs to the technical field of molecular biology, and particularly discloses a method for improving citrus canker resistance by using a CsENPP1 gene, the coding protein of the CsENPP1 gene is citrus nucleotide pyrophosphatase / phosphodiesterase 1, the coding sequence of the CsENPP1 is a nucleotide sequence shown as SEQ ID No.1, and the citrus canker resistance is improved based on overexpression of the CsENPP1 gene in citrus cells. The citrus nucleotide pyrophosphatase / phosphodiesterase 1 coding gene is integrated into citrus through an expression vector, the canker attack degree of the obtained transgenic material can be reduced to 48.9% of that of the existing citrus to the maximum extent, the canker attack degree can be remarkably reduced, the scab area is reduced, and the yield of the citrus is improved. The technology provided by the invention is a bioengineering technology which has potential to improve the citrus canker resistance, and has great value for citrus canker resistance molecular breeding.

The invention relates to an ether-containing pleuromutilin derivative as well as a preparation method and application thereof. The structural formula of the pleuromutilin derivative is shown as a formula Y. An antibacterial activity test on the pleuromutilin derivative containing ethers proves that the pleuromutilin derivative containing ethers has relatively good antibacterial activity on Xanthomonas oryzae pv. Oryzae, Pseudomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citri. And the preparation method is simple, and the production cost is low.

Disclosed herein are methods and compositions for use in controlling citrus greening, citrus canker, or fire blight, wherein the compositions comprise one or more antimicrobial peptides, or one or more polynucleotides encoding for the one or more antimicrobial peptides.

Disclosed herein are methods and compositions for use in controlling citrus greening, citrus canker, or fire blight, wherein the compositions comprise one or more antimicrobial peptides, or one or more polynucleotides encoding for the one or more antimicrobial peptides.

The invention relates to the technical field of agriculture, and particularly discloses a method for preventing citrus canker and phylloxera and application. According to the method, different prevention agents are sprayed in the spring shoot period, the flowering period, the late summer shoot period and the autumn shoot period of plants respectively, so that the purpose of efficiently preventing and treating citrus canker and phylloxera is achieved. By means of the method for preventing the citrus canker and the phylloxera, the citrus canker and the phylloxera can be effectively prevented, plants suffering from the citrus canker and the phylloxera can be treated, and the method is easy and convenient to operate and free of toxic and side effects. The invention effectively solves the problems that a single chemical agent is easy to generate drug resistance in the prior art, the research and development cost of a biological agent is high, and the prevention and treatment effects of the chemical agent and the biological agent are limited.

This invention discloses the application of the natural product santalol as an agricultural fungicide in plant disease control, belonging to the field of plant disease prevention and control. It shows good inhibitory effects against bacterial diseases such as rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwifruit canker, tobacco bacterial wilt, cucumber bacterial angular leaf spot, and potato bacterial wilt. It also shows good inhibitory effects against viral diseases such as tobacco mosaic virus; and against fungal diseases such as rice rhizome blight, wheat scab, pepper wilt, *Botrytis cinerea*, or *Colletotrichum sorghum*. It can be used in the preparation of plant fungicides. Santalol is a natural plant extract and is environmentally friendly. This invention provides a new use for the natural product santalol, enabling its application in the agricultural field for plant disease control and providing a new avenue for the research and development of new pesticides.

The invention relates to the field of pesticides, and provides an agricultural composition as well as a preparation method and application thereof. The invention provides application of an agricultural composition in preparation of a pesticide bactericide and / or a plant growth regulator. The active ingredients of the agricultural composition comprise an aloe extract and rhamnolipid. According to the present invention, the aloe extract and the rhamnolipid are adopted as the active components of the agricultural composition, and through the specific ratio, the two active components can achieve the synergistic interaction, and particularly have the excellent prevention and control effect on bacterial infected plant diseases, such as the significant effect on the prevention and control of rice bacterial streak, citrus canker and cucumber bacterial angular leaf spot, meanwhile, the growth of new shoots and new leaves of the plants can be promoted, and the method has excellent commercial value.

The invention discloses pantoea for efficiently preventing and treating citrus canker and application of the pantoea. The pantoea is named as Pantoea cypripedii Q210, the preservation number is CGMCC NO.36920, the preservation date is December 8, 2025, and the preservation site is the China General Microbiological Culture Collection Center. The bacterial strain is separated and screened from soil of a Gannan navel orange orchard, and it is found that the bacterial strain has the capacity of inhibiting growth of citrus canker pathogen Xcc, has an obvious prevention and treatment effect on citrus canker and can provide a new biological prevention and treatment means for comprehensive prevention and treatment of citrus canker.

The application discloses a method, reagent and application for identifying or tracing citrus canker bacteria. The method comprises the following steps: obtaining seven gene sequences of the to-be-tested citrus canker bacteria, performing multi-locus sequence typing analysis on the seven gene sequences, determining alleles and numbering, concatenating the allele numbers of the seven genes to serve as a sequence type, comparing the sequence type with a reference strain sequence type, and obtaining tracing information of the to-be-tested strain; the reference strain is a citrus canker bacteria with known tracing information; the seven genes are XAC_RS07980, XAC_RS21550, copB, egl, fliL, hrpA and pliY. The seven housekeeping genes suitable for multi-locus sequence typing of the citrus canker bacteria are used for tracing detection, different geographical source strains can be distinguished at high resolution, and the method has important significance for strain regional research, evolution, transmission, disease prevalence and prevention and treatment. The method can be used for identifying and tracing the citrus canker bacteria.

The application discloses a method for improving citrus canker resistance by interfering with CsRHY1A, and relates to the field of agricultural biological genes. The RNAi fragment of the CsRHY1A coding gene of citrus is cloned, the interference vector is integrated into the citrus, the transcription level of CsRHY1A is reduced, the resistance of the citrus to canker is significantly improved, the phenotype of the transgenic plant is not affected, the canker lesion area of the obtained transgenic plant can be reduced to 85% of that of the existing citrus at the most, the disease index can be reduced to 82% of that of the existing citrus at the most, the disease degree of canker can be significantly reduced, and the phenotype of the citrus is not affected, so that it is concluded that the interference of CsRHY1A can greatly improve the resistance of the citrus to canker; compared with the gene editing technology for silencing the citrus gene, the RNAi technology is more stable, the probability of obtaining a gene-silenced plant is greater, and the RNAi technology is suitable for the high-heterozygosity species of the citrus.