The invention relates to the technical field of pesticide, and particularly relates to a biological fungicide composition. The biological fungicide composition contains zhongshengrnycin and kasugamycin, wherein the weight ratio of the zhongshengrnycin to the kasugamycin is 1: (0.2-7). The fungicide composition disclosed by the invention is significant in synergized action, high in field efficacy, and wide in bacteriocidal spectrum, and has good control effect on rice blast, fusarium wilt of cucumber, cucumber downy mildew, bacterial angular leaf spot of cucumber, tomato leaf mould, tomato bacterial macula diseases, pumpkin soft rot, watermelon bacterial angular spot, bacterial blotch of watermelon, stuffed watermelon water melon, pepper anthracnose, hot (sweet) pepper bacterial leaf spot, capsicum powdery mildew, pepper grey mould, citrus canker, litchi downy blight, peach tree gummosis, scab of peach and peach shot hole. The control cost is reduced.