The invention relates to the field of research and development of biological pesticide preparations, and particularly relates to a tetramycin compound and application thereof, which are a divisional application of an invention named 'tetramycin compound and application' having an application number of 2013106143470 and being applied on November 26, 2013. The tetramycin compound comprises tetramycin and an active substance, wherein the active substance belongs to amide, including one or more of benzamides, dicarboximides, formamides, kresoxim-methyl, N-halogenated methylthio-dicarboximides and dimethomorph. Compared with the prior art, the tetramycin compound and application thereof have the advantages that the risk of resistant fungal strain selectivity can be reduced, the service life of the active substance can be prolonged, the prevention and treatment range can be enlarged, the disease prevention effect can be improved, the drug resistance of pathogenic bacteria can be retarded or overcome, the cost can be reduced, and the number of pesticide application times can be reduced.