203 results about "Alternaria" patented technology

The invention discloses a marine yeast for biologically preventing and treating postharvest diseases of fruits and vegetables, a method for preparing the same and application thereof. A marine yeast strain Rhodosporidium p aludigenum Fell & Tallman is screened out from the seawater of the coastal waters of the East China Sea. The colony of the marine yeast is pink, has a smooth surface, and grows in a culture medium added with 1 to 2 percent NaCl. The use of a cell suspension of the yeast with certain concentration can effectively inhibit various postharvest main mycoses such as alternaria, botrytis cinerea, penicilllum expansum, penicillium digitatum, and pestalotia. The marine yeast strain Rhodosporidium p aludigenum Fell & Tallman is used for biologically preventing and controlling the postharvest diseases of fruits and vegetables for the first time both at home and abroad. The strain has various functions of preventing decay, controlling oxidative stain, controlling softening flavour reversion and the like, and has the advantages of convenient use, no generation of antibiotic, no environment pollution, security, innocuity, and broad market prospect. At the same time, the strain is conducive to the export for earning foreign currency of China's high-quality fruit and vegetable products, and has significant economic and social benefits.

The present invention relates to an application of anthraquinone derivative as pesticice for controlling plant fungous diseases. The anthraquinone derivative pesticide contains one kind or several kinds of 10 anthraquinone derivative compounds. The anthraquinone derivative compounds have obvious action for preventing and curing several plant diseases, and can be used for preventing and curing economic crop, grain crop, vegetable, fruit tree and melon diseases due to fungi, including sphaerotheca, erysiphe, blumeria, fusarium, sclerotinia, pyricularia, rhizoctonia, colletotrichum, botrytis, verticillium and alternaria.

The invention discloses a method for preparing biopesticide tetramycin from Streptomyces ahygroscopicus subsp.Wuzhouensis n.subsp, and application of a product prepared by the method in preventing and controlling diseases such as apple alternaria leaf spots and the like. The tetramycin prepared by the method improves the biological potency of the tetramycin (the biological potency of fermentation liquor is improved by 15.5 percent after 0.3 to 0.5 percent of normal propyl alcohol is added.), can prolong the quality guarantee period, improves the effect of preventing and controlling apple canker to over 95 percent, has good effect of preventing and controlling the diseases such as the apple alternaria leaf spots, tomato gray mold and the like, has effect of inhibiting yeast, gram-negative and gram positive bacteria, fungi (comprising fungal pathogens) and the like, has wide adaptability, and belongs to the environment-friendly biopesticide.

The invention discloses a new Rahnella aquatilis strain HX2 (CGMCC No.1896), which is characterized by the following: manufacturing antagonistic material and IAA; inhibiting partial pathogenic bacteria (Agrobacterium, Xanthomonas, Clavibacter, Pseudomonas and Bacillus) and partial pathogenic fungus (Fusarium, Alternaria and Botrytis); detecting root cancer based on green house sunflower as indicating plant.

The invention relates to bidirectional burkholderia as well as a culture method and an application thereof and belongs to the field of plant protection (microbial pesticide). The preservation number of the bidirectional burkholderia is CCTCC NO: M2013145. The culture method of the bidirectional burkholderia comprises the following steps: adding 1ml of 2-day-aged seed bacterial strain to an LB (Lysogeny broth) culture medium with the liquid loading of 50ml under a fermentation condition, and culturing for 20-24 hours at the temperature of 25-30 DEG C at the rate of 200r/min. The bidirectional burkholderia has a remarkable inhibition effect on rice sheath blight disease, rice blast, colletotrichum musae, oxysporum, fusarium asiaticum, peronophythora litchi, sugarcane alternaria tenuis, cladosporium fulvum, xanthomonas oryzae, bacterial leaf steak and the like, can be used for the biological prevention and control of plant pathogenic bacteria, has the advantages of low toxicity, easy degradation, environmental friendliness and the like and has a bright application prospect.

The invention relates to the technical field of pesticides, and particularly relates to a bactericide composition which takes zhongshengrnycin and copper oxychloride as effective ingredients, has an obvious and enlarged bacteriocidal spectrum and no phytotoxicity, and is used for treating plant bacterial diseases as well as fungal diseases. The weight proportion of the zhongshengrnycin to the copper oxychloride is 1:5-60. The bactericide composition has obvious synergistic effect, high field prevention effect and a wide bacteriocidal spectrum, is used for preventing and treating cucumber bacterial angular leaf spot, cucumber downy mildew, tomato bacterial macula disease, tomato leaf mold, pumpkin soft rot, watermelon bacterial angular leaf spot, watermelon sponge, apple alternaria leaf spot, apple brown blotch, pear scabs, grape downy mildew, grape powdery mildew, anthracnose of grape, jujube rust diseases, jujube anthracnose, citrus canker, citrus scab, citrus anthracnose, peanut leaf spot diseases and peronophythora litchii, and has the advantages of good effect and reduction of prevention and treatment cost.

The invention discloses a Bacillus amyloliquefaciens strain used for biocontrol, and a use thereof. The Bacillus amyloliquefaciens strain is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.13466. The Bacillus amyloliquefaciens strain is prepared through separation culture of the strain and 16S rDNA molecular identification. The Bacillus amyloliquefaciens is cultured to obtain different active substances (including a fermentation liquid, a supernatant and a bacterial suspension). The different active substances have an inhibition effect on decayed fungi (blue moulds and alternaria) on the surfaces of common fruits in vitro; and the strain can be used to anticorrosion during the storage of loquats, pears, cherry tomatoes and apples. The screened Bacillus amyloliquefaciens strain and products thereof can effectively inhibit the postharvest corruption of various fruits, and can be used for preparing a biocontrol fresh-keeping agent able to inhibit blue moulds, alternaria and other decayed fungi and with wide bacteriostasis spectrum.

The invention relates to a sterilizing composition which is a suspending agent comprising active components of Tebuconazole and iprodione, wherein the weight percentage of the active components of the Tebuconazole and the iprodione is 1-60:1-60. The invention is used for preventing and curing general diseases on crops and fruit trees, has extremely remarkable effects of preventing and curing rice sheath blight disease, sigatoka, apple alternaria leaf spot and has obvious effect higher than single agents. The sterilizing agent can generate higher synergistic effect, overcomes and retards the disease drug resistance, broadens the preventing and curing spectrum, reduces the dosage and the drug treatment times, decreases the cost and lightens the toxicity.

The invention discloses an alternaria alternate spore suspension liquid and an application thereof. The alternaria alternate spore suspension liquid is prepared through germ separation, germ cultivation, spore production through induction, and preparation of spore suspension liquid. The alternaria alternate spore suspension liquid is used in measurement of tobacco brown spot, or in screening of drug resisting the tobacco brown spot, biocontrol bacterium or natural products, or in research of soil ecology of the alternaria alternate and germ infect circulation. The alternaria alternate spore suspension liquid adopts a multipoint inoculation method, has fast cultivation speed and short cultivation cycle; the alternaria alternate is promoted to generate large quantity of spores rapidly through the comprehensive action of methods such as low temperature high humidity stimulation, gradient cooling, dark induction and the like, and the quantity of the spore generated is 50-100 times that of conventional spore generation quantity. The alternaria alternate spore suspension liquid is light in contamination, the time for cultivating hypha and generating spore through induction is relatively fixed and concentrated, spore generation and growing are synchronous, and the spores prepared in the same batch and different batches are small in quality difference and more stable in quality.

The present invention relates to protein for improving disease resistance and enhancing plant growth, and the encoding gene. The present invention provides a type of protein PEAt1 separated from chain Alternaria. The protein has the function of improving disease resistance and enhancing plant growth. The present invention also relates to the preparation method and uses of the protein and multi nucleotide acid, and the expression carrier and transformer constructed by the gene of the multi nucleotide acid.

The invention belongs to the pesticide field, and concretely relates to an agricultural phytosterol bactericide. The bactericide contains 3-70% of an effective active component. Phytosterol can be added into a wetting agent, a dispersant, a filler and the like to prepare a wettable pulvis, can be added into antifreeze agent, a wetting dispersant, a thickening agent, an antiseptic and water in proportion to prepare a suspending agent, can also be added into an emulsifier, a solvent and water in proportion to prepare an emulsion in water. The agricultural phytosterol bactericide can be used for controlling crop, fruit and vegetable diseases comprising Cytospora sp, Alternaria oleracea, Coniella diplodiella and the like. According to the invention, phytosterol is from plants, is well compatible to the environment and is easy to degrade, so the phytosterol bactericide is a botanical bactericide and has a wide market prospect.

The invention relates to a multifunctional decontaminating column used for detecting alternaria spp toxin and a use method thereof. The multifunctional decontaminating column comprises reversed phase chromatographic packing and cation-exchange chromatography packing. The multifunctional decontaminating column can be suitable for various fruit samples, various vegetable samples, various grain samples, various feed samples and various food samples.

The invention provides a walnut green seedcase extract compound preservative as well as a preparation method and application thereof. The walnut green seedcase extract compound preservative comprises the following components in percentage by weight: 0.01-9% of walnut green seedcase extract, 0.5-3% of chitosan, 0.05-0.12% of emulsifying agent and the balance of water. The preparation method of the compound preservative comprises the following steps: respectively adding the walnut green seedcase extract and chitosan into water according to the weight percentage, and dissolving all the components; sufficiently stirring the components to be uniform; adding the emulsifying agent, and carrying out ultrasonic dispersion to obtain the walnut green seedcase extract compound preservative. The walnut green seedcase extract compound preservative prepared by using the method is used for inhibiting one or a combination of several of penicillium, alternaria, rhizopus stolonifer, botrytis cinerea and fusarium. The walnut green seedcase extract compound preservative provided by the invention is safe, efficient, stable in performance and simple in preparation process.

The invention provides a LAMP-LFD (loop-mediated isothermal amplification-lateral flow dipstick) detection method of rape Leptosphaeria maculans, which comprises the following steps: designing three pairs of LAMP primers FIP, BIP, LF, LB, F3 and B3, wherein FIP is a 5'-terminal biotin labeled primer, and LF is a 5'-terminal fluorescein isothiocyanate (FITC) labeled probe; and preparing an LAMP reaction system containing the three pairs of primers and a sample template, amplifying the LAMP reaction system, carrying out LFD detection, and carrying out specificity and sensitivity evaluation. The result shows that the lowest detection line of the LAMP-LFD method for the L.maculans genome DNA (deoxyribonucleic acid) is 114fg/mu L. In the specificity verification experiment, when five related phytopathogens, including Alternaria, Lasiodiplodia theobromae, fusarium and Diaporthe phaseolorum var. caulivora, are used and Diaporthe phaseolorum var. meridionalis and L.maculans are used as specific test strains, the LAMP-LFD detection method only has positive reaction for the L.maculans.

The invention relates to an alpha-L-rhamnosidase gene, especially relates to an alpha-L-rhamnosidase gene from alternaria and an application thereof, and belongs to the technical field of gene engineering. An alpha-L-rhamnosidase gene has a nucleotide sequence as shown in SEQ ID No. 1. An alpha-L-rhamnosidase coded by the alpha-L-rhamnosidase gene has an amino acid sequence as shown in SEQ ID No. 2. The alpha-L-rhamnosidase gene of the invention can be cloned on a Ppic9K expression vector; pichia yeast GS115 is transformed; expression is induced by methanol; and the enzyme yield of fermentation broth is 426 mg/L. The recombinase of the alpha-L-rhamnosidase gene expressed in pichia yeast can efficiently hydrolyze the main bitter substance of naringin in citrus fruit juice, can synthesize transfructosylation products by using rhamnose as a donor and using various substances as receptors, and has important potential application value in food, pharmacy and chemical industry.

The invention relates to a paper pulp production method by utilizing alternaria tenuis to biodegrade lignin. Combined with machining, the method comprises steps of: immersing fragmented raw materials into a water tank containing alternaria tenuis bacterial liquid; maintaining a temperature at 30-45 DEG C; and decomposing for 6-48 h. The production process of the invention uses no chemical, generates no pollution, saves water, has low material loss, low energy consumption and a cost only 40% of that of an existing chemical pulping method. The method can be used to produce paper pulp using raw materials of wheat straw, straw, reed, Chinese alpine rush, bagasse, bamboo and timber, etc., and realizes zero discharge of water for paper pulp production. Produced paper pulp has excellent quality and no chemical toxin, and can be used for manufacturing of daily used paper like food package paper and medicine packing paper, etc.; besides the production process has no pollution. The method has high economic benefit and social benefit, and is a high-tech with value for ecological balance.

The invention discloses a sterilization composite whose effective activity components compries triflumizole and chlorothalonil in a weight ratio of 1-20 to 80-1%. Auxiliary agent and excipient are added into the effective activity components to prepare wettability powder, water-dispersion granule and suspending agent. The sterilization composite is mainly used for preventing and curing powdery mildew, cladosporium cucumerinum and alternaria leaf spot of grain, vegetable and fruit trees.

The invention discloses a sterilization composite whose effective activity components are comprise flutriafol and triflumizole in a weight ratio of 1-60 to 60-1. Auxiliary agent and excipient are added into the effective activity components to prepare wettability powder, water-dispersion granule and suspending agent. The sterilization composite is mainly used for preventing and curing powdery mildew, cladosporium cucumerinum and alternaria leaf spot of grain, vegetable and fruit trees.

An isolongifolenone oxime ether derivative is prepared by the following steps of: taking isolongifolenone as the raw material, synthesizing isolongifolenone oxime through addition-elimination reaction, and performing alkylation reaction of the isolongifolenone oxime. Simultaneously, the isolongifolenone oxime ether derivative disclosed by the invention can inhibit bacteria, such as staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, bacillus proteus vulgaris and pseudomonas aeruginosa, and fungus, such as Aspergillus niger, exserohilum turcicum, corn sheath blight, alternaria musae Bovr.et Bat. and colletotrichum musae; and the isolongifolenone oxime ether derivative has the excellent insecticidal action for insect pests, such as aphid and rice planthopper.

The invention discloses a vegetable preservative and a preparation method thereof, and relates to the field of crop preservation. A vegetable preservative comprises 0.3%-0.5% citric acid and 0.3%-0.6% chlorine in terms of mass percentage. Calcium chloride, 0.5-0.7% sodium phosphate, 0.1%-0.3% vitamin C, 5%-10% oligosaccharides, 2% surfactant, 0.5%-1% konjac powder solution, and the balance is water, wherein all The oligosaccharides are cultivated by inoculating Botrytis cinerea and Alternaria on the culture medium. With the technical scheme of the present invention, the preparation steps are reasonable and easy to operate, and the prepared vegetable preservative can improve the disease resistance of vegetables without It is non-toxic and harmless, and has a good fresh-keeping effect.

The invention discloses a bactericidal composition containing triflumizole and antibiotic compounds, which comprises an active component A and an active component B, wherein the active component A is triflumizole, the active component B is selected from one of the following bactericides: validamycin, polyxins and kasugamycin, and the ratio of the active component A to the active component B in parts by weight is (1-50): (1-50). The composition can prevent and treat a plurality of crop diseases, has obvious synergistic action, expands bactericidal spectrum, has higher activity on crop diseasescomprising rice blast, powdery mildew, downy mildew, banded sclerotial blight, gray mold, leaf mold, blight, epidemics and alternaria leaf spot, decreases the dosage of pesticides and the residue of pesticides on crops, and reduces environment pollution.