41 results about "Alternaria" patented technology

The application discloses application of BoDMR1 and BoDMR6 genes in cultivating disease-resistant broccoli varieties, an expression vector and a cultivating method, and relates to the technical field of plant molecular biology. A nucleotide sequence of a broccoli BoDMR1 gene is shown in SEQ ID No: 7, or a nucleotide after gene mutation of the nucleotide sequence shown in SEQ ID No: 7; and a nucleotide sequence of a broccoli BoDMR6 gene is shown in SEQ ID No: 8, or a nucleotide after gene mutation of the nucleotide sequence shown in SEQ ID No: 8. Through gene editing on endogenous BoDMR1 and / or BoDMR6 genes of plants, mutant plants showing resistance to diseases such as sclerotinia, black rot and alternaria blight can be obtained. Therefore, the application is beneficial to providing good resources for cultivating disease-resistant broccoli varieties.

The application discloses a bacillus velezensis A13 wettable powder and application thereof, and belongs to the technical field of biological pesticides. The application aims to solve the problems of limited prevention and treatment effect of existing seed treatment, high labor intensity of foliar spraying and short effective period. The wettable powder comprises a mother powder prepared from fermentation liquor and diatomite, and a dispersant, a wetting agent, a stabilizer and a protective agent, wherein the total addition amount of the dispersant and the wetting agent is 5%-14% of the mass of the mother powder, the addition amount of the protective agent is 0.5%-2% of the mass of the mother powder, and the addition amount of the stabilizer is 1%-3% of the mass of the mother powder. The application can be used for preventing and treating root diseases caused by panax quinquefolius rust rot fungus and leaf diseases caused by panax quinquefolius alternaria leaf spot fungus, and promoting plant growth.

This invention belongs to the field of pharmaceutical technology, and relates to the use of a combination of sour cherry powder and celery seed powder, and complexes containing this composition. Specifically, the sour cherry powder and celery seed powder composition is used in the preparation of drugs for treating or regulating intestinal flora imbalance caused by hyperuricemia. The preparation method of this composition is as follows: pitting sour cherries, freezing them separately with liquid nitrogen, grinding them into powder using a pulverizer, and sieving them separately to obtain sour cherry powder and celery seed powder; mixing the obtained sour cherry powder and celery seed powder at a ratio of (0.5~3):1. This invention discusses the diversity, richness, and evenness of intestinal flora through diversity analysis, community bar chart analysis, and Venn diagram analysis. The results show that this composition can increase beneficial bacteria (Prevotella, Mulliez, Lactobacillus, etc.), reduce harmful bacteria (Alternaria), improve the structure of intestinal flora, and maintain the balance of intestinal flora.

The invention belongs to the technical field of agriculture, and particularly relates to a degerming and seedling formation solution, a preparation method, an application method and a method for promoting seed germination and seedling formation. The degerming and seedling formation solution provided by the invention comprises gibberellic acid, indoleacetic acid, naphthylacetic acid, coptis chinensis and carbendazim, and the combination of coptis chinensis extract, carbendazim and growth hormone (gibberellic acid, indoleacetic acid and naphthylacetic acid) in a specific proportion has a synergistic inhibition effect on alternaria alternata and has a promotion effect on seed germination and seedling formation. The invention provides the preparation method of the degerming and seedling forming solution, and the application is simple. According to the application method of the degerming and seedling forming solution, the operation steps are simple, convenient and efficient, and the seedling forming efficiency is remarkably improved. The result of the embodiment shows that the germination rate of the Sinojackia xylocarpa seeds reaches 100% after the optimal treatment is performed for 15 days, and the survival rate of the seed seedlings reaches 96.67% after the optimal treatment is performed for 30 days.

This invention belongs to the field of biological agent technology, specifically relating to a strain of *Trichoderma*, a biocontrol agent, a bio-organic fertilizer, and methods and applications for controlling plant diseases. This invention provides a strain of *Trichoderma* P11, with accession number CCTCC NO: M 2024648. The *Trichoderma* P11 of this invention can enhance positively correlated disease resistance genes in plants. PnCAD , PnSN1 , PnDEFL1 The relative expression level of *Trichoderma* P11 was increased, enhancing the activity of disease resistance-related enzymes SOD, POD, and CAT. Furthermore, *Trichoderma* P11 could damage the cell membrane of pathogens, inhibiting their growth. *Trichoderma* P11 also exhibits good rhizosphere colonization ability. The results of the examples show that *Trichoderma* P11 of the present invention can reduce the incidence of root rot in Panax notoginseng fields and can also antagonize various pathogens such as *Pseudomonas aeruginosa*, *Pseudomonas oryzae*, *Geotrichum candida*, *Early Blight of Tomato*, *Phytophthora indicum*, *Phytophthora capsici*, and *Alternaria spiralis*.

The application discloses a pesticide composition containing fluopyram and application of the pesticide composition in prevention and treatment of apple alternaria leaf spot, and belongs to the technical field of pesticides. A volatile oil extract of Alpinia galanga and a volatile oil extract of Eupatorium fortunei are obtained, and in indoor tests, the colony diameters of apple Alternaria alternata are all reduced, which indicates that the volatile oil extract of Alpinia galanga and the volatile oil extract of Eupatorium fortunei can inhibit the mycelium growth of apple Alternaria alternata. The volatile oil extract is used as a synergistic extract, combined with fluopyram, and added with various additives to prepare the pesticide composition containing fluopyram. In field tests, the disease index of fruit trees is all reduced, which indicates that apple Alternaria alternata is inhibited, and the relative prevention and treatment efficiency of the compound group is the highest and reaches 80.05%. The pesticide composition containing fluopyram is prepared by compounding fluopyram and the synergistic extract containing the volatile oil extract of Alpinia galanga and the volatile oil extract of Eupatorium fortunei, the pesticide composition can better inhibit apple Alternaria alternata, and is suitable for development of a pesticide for preventing and treating apple alternaria leaf spot.

The invention discloses a grape wine color protection method based on alternaria MG1, and belongs to the technical field of fermented wine brewing. According to the method, alternaria MG1 is taken as a research object, a phenylpropane metabolic pathway of alternaria MG1 is activated through various stimulation methods such as ultraviolet radiation, exogenous addition of oligomeric proanthocyanidins, co-culture with other endophytic filamentous fungi and the like, and the alternaria MG1 and saccharomyces cerevisiae are subjected to mixed fermentation, so that an optimal color protection process for grape wine color is explored. The invention deeply discusses the influence of the mixed fermentation on the color and the related quality characteristics of the wine, provides a new theoretical support and a process optimization strategy for the application of the multi-strain synergistic fermentation based on the grape endophyte in wine brewing, and has important guiding significance for production practice.

The present invention relates to a method for controlling or preventing infestation of a plant by a phytopathogenic microorganism of the genus Fusarium, Aceturium, Corynespora, Alternaria or Colorospora, the method comprising applying a combination or composition to the plant, its locus or its propagation material in any desired sequence or at the same time, the combination or composition comprises as component (A) a compound of formula (A-1) and as component (B) a compound of formula (B-1). Compositions suitable for use in the invention are also disclosed. (A-1) is greater than (B-1).

The present application provides an endophytic bacterial strain with a high camptothecin yield, which is Alternaria temuissima ZCMUKL-S1, deposited as CCTCC NO: M2021189, or Alternaria citricancri ZCMUKL-S2, deposited as CCTCC NO: M2021190, or Aspergillus versicolor ZCMUKL-S3, deposited as CCTCC NO: M2021191. The endophytic bacterial strain has a high yield of camptothecin, wherein the yield of camptothecin per unit dry mycelium powder of Aspergillus versicolor ZCMUKL-S3 is as high as 116 μg / g, which is the highest yield of camptothecin produced by endophytic bacterial strains reported at present, and has potential application value for its industrial production. In addition, the endophytic bacterial strain which can produce high camptothecin provided by the present application has good genetic stability.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to bacillus BFI-L3 with a broad-spectrum antibacterial effect and application of the bacillus BFI-L3. The invention discloses bacillus velezensis BFI-L3, which is bacillus velezensis, and the preservation number of the bacillus velezensis BFI-L3 is CGMCC (China General Microbiological Culture Collection Center) No.35399. The strain has broad-spectrum antibacterial activity, has a remarkable antibacterial effect on camellia anthracnose, botryosphaeria, alternaria and other pathogenic fungi, and can synchronously prevent and control the compound diseases of the tea garden. The strain also has plant growth promoting ability, VOCs generated by the strain can significantly promote the growth of arabidopsis thaliana, and high-quality microbial resources are provided for green prevention and control of tea gardens and biological prevention and control of plant diseases.

The application relates to a Burkholderia gladioli NDG19 and an application of the Burkholderia gladioli NDG19 in disease resistance, and belongs to the technical field of microorganisms. The Burkholderia gladioli NDG19 is preserved in the Guangdong Provincial Microbial Culture Collection Center on July 31, 2023, the strain preservation number is 63701, and the preservation address is No. 59 Building, 5th Floor, Guangzhou, Guangzhou, China. The Burkholderia gladioli NDG19 provided in the application can effectively inhibit the growth and reproduction of Fusarium oxysporum and Alternaria tenuis, and can effectively prevent and treat plant fusarium wilt such as sharp banana fusarium wilt and melon root rot of cucurbitaceae crops, solves the problems of environmental pollution and food safety caused by chemical fungicides, the strain has the functions of iron carrier production, phosphorus dissolution and potassium dissolution, can effectively improve the utilization rate of phosphorus and potassium in soil as a biological bacterial fertilizer, promotes the growth of plants, is safe and environment-friendly, and has a wide application prospect.

This invention discloses a lysinibacillus macroides bacterium that degrades Alternaria alternata toxins and its applications, belonging to the field of microbial technology. The lysinibacillus macroides is deposited at the China Center for Type Culture Collection (CCTCC), Wuhan University, Hubei Province, with accession number CCTCC No. M20241816. This invention is the first to obtain a lysinibacillus macroides bacterium that can effectively reduce and degrade Alternaria alternata toxins, which helps improve the quality of agricultural products (including aquatic products).

The invention discloses a biological treatment and recycling method for synchronously removing carbon and enriching lithium from oil and gas field produced water, which comprises the following steps: 1) inoculating microorganisms into a liquid culture medium, and culturing to obtain a microorganism culture solution or culture; 2) directly adding and / or treating the microbial culture solution and / or culture obtained in the step 1) and then adding the microbial culture solution and / or culture into a reactor filled with oil and gas field produced water to be treated; (3) operating the reactor in the step (2), degrading organic matters in the oil and gas field produced water by microorganisms and enriching lithium ions; and 4) separating the microorganisms from the oil and gas field produced water, and extracting lithium ions enriched in the microorganisms. Wherein the microorganism is selected from one or a combination of more of the following microorganisms: staphylococcus saprophyticus, penicillium fistulosum, bacillus altitudinis, alternaria tenuissima and aspergillus sydowii. According to the method, recycling of lithium is achieved while oil and gas field produced water is recycled / discharged after reaching the standard, and the method has important significance on the national ecological environment and the new energy strategy.

This invention relates to the field of biomedical detection technology, specifically to plasma circulating microbial biomarkers for early screening of gastric cancer and their applications. The plasma circulating microbial biomarkers include: *Ralstonia solanacearum*, *Staphylococcus aureus*, *Alternaria metachromatica*, *Porphyromonas gingivalis*, *Lactobacillus reuteri*, *Rhodococcus erythropolis*, *Akkermansia muciniphila*, *Lactobacillus gasseri*, *Bifidobacterium bifidum*, and *Neisseria gonorrhoeae*. This invention screens and, for the first time, combines 10 specific microbial biomarkers with Low Pass technology. WGS technology can achieve detection results at low depths, significantly reducing screening costs while ensuring detection sensitivity, and providing a brand-new solution for early gastric cancer screening.

The invention discloses an instant wrinkle-removing skin-care composition which comprises the following components in parts by weight: 1-5 parts of alteromonas fermentation product extract; 0.2 to 2 parts of an arnica flower extract; 0.02 to 0.2 part of arginine / lysine polypeptide; and 0.1 to 1 part of Euglena parvata (EUGLENA GRACILIS) polysaccharide. The invention also discloses an application of the instant wrinkle-removing skin-care composition in cosmetics. The instant wrinkle-removing skin-care composition provided by the invention can rapidly permeate into eye skin to achieve an instant wrinkle-removing effect, and the safety and the stability of the product are ensured while the composition is comparable with medical beauty.

The invention discloses a strain for degrading alternaria toxin and application of the strain, and relates to the technical field of microorganisms. The disclosed biocontrol bacterium MM14-1 is identified to be trichoderma harzianum through ITS, the trichoderma harzianum MM14-1 shows that the trichoderma harzianum MM14-1 has a good inhibition effect on growth of alternaria alternata in a degradation test on a liquid culture medium, the degradation rate reaches 96%, meanwhile, the degradation rate of an intracellular crude extract of the trichoderma harzianum MM14-1 on alternaria alternata toxin is 98.04%, and the trichoderma harzianum MM14-1 can be used for preparing the trichoderma harzianum. The intracellular crude extract of trichoderma harzianum MM14-1 can inhibit synthesis of alternaria tenuissima toxin, can be applied to prevention and treatment of alternaria tenuissima and alternaria tenuissima toxin, can overcome the problems of environmental safety, health safety and the like caused by use of chemical pesticides, is beneficial to pollution-free production of agricultural products, and can improve the product quality. According to the method, a strain capable of degrading alternaria toxin more efficiently, safely and stably is provided, and a breakthrough from bacterium prevention to detoxification is achieved.

The invention provides lactobacillus plantarum, lactobacillus paracasei, a complex microbial inoculant thereof and application of the complex microbial inoculant and the complex microbial inoculant in cherry fruit preservation, and belongs to the technical field of fruit and vegetable biological preservation. The lactobacillus plantarum TZ28 and the lactobacillus paracasei TZ13, which are screened out by the invention, have broad-spectrum antibacterial effects and have safety and excellent probiotic characteristics. The whole culture solution, the bacterial suspension and the cell-free fermentation supernatant of the lactobacillus plantarum TZ28 and the lactobacillus paracasei TZ13 can inhibit the alternaria alternata separated from the rotten cherries. A bacterial suspension formed by combining lactobacillus plantarum TZ28 and lactobacillus paracasei TZ13 according to a ratio of 1: 1 is used as a biological fresh-keeping agent, so that the decay rate and weight loss rate of cherry fruits can be effectively reduced, the content and hardness of soluble solids are maintained, the aging decay of cherries in the storage period is effectively slowed down, the quality of the cherries is obviously improved, the storage period of the fruits is prolonged, and the quality of the cherries is improved. The nutritional value and the health benefits of the cherry fruits are enhanced.

The invention relates to a preparation method of a mortierella alpina agent, which comprises the following steps: inoculating mortierella alpina into a culture medium for fermentation culture, centrifuging, and taking supernate; the name of the bacterial strain of the mortierella alpina is Mortierella alpina RS-01, and the preservation number of the mortierella alpina is CGMCC (China General Microbiological Culture Collection Center) No.41944. The invention further discloses a preparation method of the mortierella alpina. The mortierella alpina agent provided by the invention can be used for preventing and treating pathogenic bacteria of ginseng rust rot, pathogenic bacteria of muskmelon fusarium wilt, pathogenic bacteria of melon and fruit rot, pathogenic bacteria of apple fruit rot, pathogenic bacteria of northern corn leaf blight, pathogenic bacteria of seedling blight, pathogenic bacteria of walnut canker, pathogenic bacteria of poplar canker and pathogenic bacteria of pepper anthracnose; the compound has the best antibacterial effect on pathogenic bacteria of blueberry stem canker, pathogenic bacteria of apple shell monochromatic alternaria, pathogenic bacteria of tobacco brown spot, and especially pathogenic bacteria of ginseng rust rot.

The invention belongs to the technical field of fermentation engineering and plant disease prevention and control, and particularly relates to pseudomonas veronii and application thereof in prevention and control of plant fungal diseases. The invention discloses Pseudomonas veronii JD003 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36825. The test shows that the Pseudomonas veronii JD003 can effectively inhibit the growth of tomato root rot, grape botrytis cinerea, colletotrichum capsici and alternaria capsici, and can effectively inhibit the growth of the tomato root rot, the grape botrytis cinerea, the colletotrichum capsici and the alternaria capsici. The compound provided by the invention has the advantages that the inhibition effects on the corresponding pathogenic microorganisms such as fusarium solani, botrytis cinerea, colletotrichum oxysporum and alternaria hexabarzicola respectively reach 64.7%, 72.9%, 74.1% and 73.8%, the compound has a good inhibition effect on pepper anthracnose, the field prevention and control effect reaches 83.5%, and a new direction is found for preventing and controlling plant diseases.

The invention discloses alternaria BP-1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the alternaria BP-1 is CGMCC (China General Microbiological Culture Collection Center) NO.41528. The alternaria BP-1 can be used for preventing and controlling weed bolboschoenus planiculmis in paddy fields. The alternaria BP-1 provided by the invention is separated from naturally diseased leaves of weeds in a paddy field, and the strain is separated from the natural environment, so that the ecological safety problem is not caused. The alternaria BP-1 can infect weed bolboschoenus planiculmis and is high in pathogenicity, obvious leaf disease spots can be formed 7 days after inoculation, and the purpose of effectively controlling weeds is achieved along with expansion of the disease spots. The bacterium is safe to rice and high in specificity, and has great development potential in prevention and treatment of rice field weed bolboschoenus planiculmis.

This invention relates to the field of molecular diagnostics and early tumor screening technology, specifically disclosing plasma circulating microbial biomarkers for early lung cancer screening and their applications. The biomarkers include: Staphylococcus aureus, Fusobacterium nucleatum, Klebsiella pneumoniae, Alternaria incomplexa, Legionella pneumophila, Neisseria mucosa, Ralstonia pickettii, Pasteurella multocida, and Haemophilus parainfluenzae. This invention screens and combines nine specific microbial biomarkers with low-pass whole-genome sequencing technology. The WGS (World Gastroenterology) assay detects nine characteristic microbial biomarkers and uses machine learning algorithms to build a high-precision diagnostic model. The established diagnostic model has excellent stability and clinical applicability, which can not only achieve efficient identification of early lung cancer, but also significantly reduce detection costs.

The application of leidinmycin compounds in inhibiting plant pathogenic fungi and preventing and treating plant diseases belongs to the technical field of pesticide fungicides. In order to screen leidinmycin compounds capable of being used for preventing and treating plant diseases caused by plant pathogenic fungi or plant pathogenic oomycetes, five kinds of leidinmycin compounds are used for inhibiting plant pathogenic fungi and preventing and treating plant diseases. The leidinmycin compounds have strong inhibitory effect on plant pathogenic fungi, including the growth of fungal hyphae in Sclerotinia, Rhizoctonia, Elsinoe, Botrytis and Alternaria and the growth of oomycete hyphae in Pythium, Peronospora and Pseudomonas. In addition, the leidinmycin compounds have good prevention and treatment effect on plant diseases caused by the above plant pathogenic fungi and plant pathogenic oomycetes, and have no toxic side effect on plants, and can be developed and applied as effective components of pesticides or plant pathogenic fungicides.

The invention discloses a preparation method of a lipopeptide mixture and an antibacterial effect determination method, relates to the technical field of lipopeptide mixtures, and realizes efficient preparation and accurate identification of the lipopeptide mixture by optimizing fermentation, separation and purification processes of bacillus velezensis TZ01 and combining genome sequencing and liquid chromatography-mass spectrometry technologies. The antibacterial effect of the lipopeptide mixture is systematically verified, the provided preparation method of the lipopeptide mixture is clear in process and mild in condition, complex equipment and harsh reaction conditions are not needed, accurate identification of lipopeptide components is achieved through a genomic sequencing and liquid chromatography and high-resolution mass spectrometry combined technology, and the application prospect is wide. According to the present invention, the antibacterial spectrum covers the main citrus pathogenic bacteria such as Pseudomonas solani, Alternaria alternata, Colletotrichum gloeosporioides, Penicillium digitatum, Penicillium italicum and the like, such that the requirement of the comprehensive prevention and control of various citrus diseases can be met, the defect of the narrow antibacterial spectrum of the existing lipopeptide antibacterial agent is solved, the lipopeptide substance is derived from the microbial metabolism, the chemical pesticide residue risk does not exist, and the application prospect is broad. Ecological environments such as soil and water cannot be polluted.

The application discloses a kind of agrobacterium-mediated panax ginseng alternaria gene transformation methods, comprising: (1) co-culture: after being activated and induced to cultivate, the recombinant agrobacterium containing target gene is co-cultured with panax ginseng alternaria mycelium suspension in IM culture medium;(2) screening: after co-culture, when 5-8 panax ginseng alternaria colonies are observed, the mycelium area of colony accounts for 30%-60% of the total area of IM culture medium, while the number of agrobacterium plaque is not more than 10, pour a layer of PDA solid medium containing screening antibiotic on IM culture medium to carry out double-layer screening;(3) verification.The agrobacterium-mediated panax ginseng alternaria gene transformation method of the application can effectively inhibit the excessive growth of agrobacterium and greatly improve the true positive rate, and realizes the best balance of agrobacterium infection activity and fungal tolerance, avoids the death of fungal cells due to excessive infection, or transformation failure due to insufficient infection, provides a reliable technical means for transforming panax ginseng alternaria strain and verification.

This invention discloses a disease-resistant and growth-promoting strain of *Streptomyces oryzae* P9-1 and its applications. *Streptomyces oryzae* P9-1 has the accession number CGMCC No. 36797 at the China General Microbiological Culture Collection Center. This strain exhibits typical actinomycete characteristics, excellent salt tolerance, and possesses multiple enzyme activities and potassium-solubilizing activities. Antagonism tests show that this strain has significant inhibitory effects on *Alternaria maculata*, the pathogen of apple tree rot, and *Alternaria spp.*, the pathogen of apple leaf spot. Growth-promoting tests show that this strain can significantly increase the plant height, stem diameter, fresh weight, and various root morphological indicators of maize seedlings. This strain can be used to prepare biocontrol agents for apple diseases and growth-promoting agents for maize, possessing dual application value in both biological control and crop growth promotion.

The invention discloses a single spore isolation method of alternaria alternata, which comprises the following steps: collecting disease tissues infected by the alternaria alternata, and culturing the alternaria alternata; preparing a culture medium for growth of alternaria alternata; melting the end part of the clean glass capillary tube, and stretching the glass capillary tube into a sharp needle shape; under a microscope, a glass needle is used for picking single alternaria alternata spores from the disease tissue subjected to moisturizing culture, the single alternaria alternata spores are transferred into a culture medium to be cultured, and a pure culture is obtained and serves as a basis for purification and identification. According to the method, the borosilicate glass capillary tube is adopted and can be repeatedly burnt, melted and drawn to be manufactured into a key tool glass needle required by single spore separation, materials are effectively saved, meanwhile, the burning and melting process is the sterilization process, and the operation process is saved. By optimizing preparation of a single spore separation tool, the separation efficiency of alternaria alternata can be remarkably improved, the experimental period is shortened, the experimental cost is saved, the success rate of single spore separation is increased, and the single spore separation process of fungi is quicker, more efficient and more accurate.

This invention relates to the field of cultural relic protection technology, specifically to a plant-based antibacterial agent for the prevention and control of microorganisms in earthen sites and its screening method. The antibacterial agent uses citral as its core component and ensures high antibacterial efficiency through a systematic screening method (including microbial identification, inhibition zone testing, MIC / MBC determination, and persistence assessment). Experiments show that citral has strong inhibitory effects on common pathogens in earthen sites (such as Alternaria and Mucor), and a 6% concentration can achieve at least 7 days of sustained antibacterial activity. This invention is green and safe, providing a reliable solution for the prevention and control of microorganisms in earthen sites.

The invention discloses a novel meroterpenoid compound as well as a preparation method and application thereof. The structural formula of the compound is shown as a formula I. The compound is separated from an Alternaria sp. XZ146 solid fermentation extract for the first time, and the antiviral activity of the compound is reported for the first time. Experimental results show that the compound in the formula I can be used for preparing an antiviral preparation, especially an anti-influenza A preparation.

The invention relates to bacillus velezensis and application thereof. The bacillus velezensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36579. According to the present invention, the compound can be used for preventing and controlling at least one pathogenic bacterium selected from the group consisting of Ilyonectria, Sclerotinia sclerotiorum, Botrytis cinerea, Alternaria alternata, Rhizoctonia, Fusarium sp. And Colletotrichum gloeosporioides, and further can be used for promoting the growth and / or improving the soil fertility, and can be used for preventing and controlling at least one pathogenic bacterium selected from the group consisting of Sclerotinia sclerotiorum, Botrytis cinerea, Alternaria alternata, Rhizoctonia, Fusarium sp. And Colletotrichum gloeosporioides.