130 results about "Downy mildew" patented technology

The invention discloses a vegetable disease incubation period detection method and system based on bimodal time sequence collaborative fusion, and the method comprises the steps: obtaining a leaf image through building an acquisition environment, constructing a training sample data set, and constructing a downy mildew incubation period spectral feature adaptive enhancement (AW-FPF) module; the method comprises the following steps: decomposing a hyperspectral signal into low-frequency and high-frequency components through spectrum time sequence adaptive wavelet decoupling, obtaining an enhanced feature tensor through spectrum multi-scale pathological feature frequency-time dual-path aggregation fusion frequency domain and time domain paths, and obtaining a first feature sequence through weighted screening by using a multi-head attention mechanism; a downy mildew incubation period prediction (HyChl-TFNet) model containing a hyperspectral branch, a chlorophyll fluorescence parameter branch, a feature fusion branch and a classifier is constructed, bimodal features are processed and fused to output a day number prediction result, accurate recognition of the downy mildew incubation period is achieved, the detection precision can be controlled to the day number level, and the detection accuracy is improved. And an accurate time basis is provided for early prevention and control of diseases.

The invention relates to the technical field of image recognition, in particular to a horticultural product fungal disease image recognition system and method, and the system comprises an image collection module, an image segmentation module, an anomaly detection module, a feature extraction module, a disease recognition module and a decision output module. In the prior art, the intensity of a light supplementing lamp is manually adjusted, and instantly changing illumination conditions (such as cloud layer shielding and tree shadow shaking) are difficult to deal with in an open-air plantation, so that leaf scab details are often submerged by strong light or dim light is blurred; according to the scheme, a closed-loop linkage system of ambient light perception and an annular LED is innovatively deployed, and the light supplementing intensity is reversely adjusted in a millisecond level by tracking ambient brightness in real time, so that purple gray hyphae of downy mildew in mist in the morning and anthracnose black spots under the noon burning sun present completely consistent color saturation and texture definition in imaging; and the problem of misdiagnosis caused by illumination fluctuation in the traditional method is thoroughly solved.

The invention relates to the technical field of agriculture, and discloses a biological organic bacterial fertilizer capable of improving plant disease resistance, which comprises the following components in percentage by mass: 30-50% of bacillus liquid; 30%-50% of a trichoderma bacteria solution; 5%-10% of a chitosan solution; 5%-15% of a sodium alginate solution; 2%-5% of a composite solution; 1% to 5% of a nanocomposite; the composite solution comprises the following components in percentage by mass: 15%-18% of artemisia capillaris; 7%-12% of tripterygium wilfordii; 9%-17% of radix astragali; 15%-19% of fructus cnidii; and 12%-17% of raw licorice. Through effective combination of traditional Chinese medicine components and beneficial bacteria, the insect pest resistance and disease resistance of crops are remarkably enhanced, breeding of aphids, red spiders and other pests can be effectively inhibited, fungal diseases such as downy mildew, powdery mildew and the like are prevented, the immunoreaction of plants is enhanced, and the disease incidence rate is fundamentally reduced.

The invention discloses a grape downy mildew resistance whole genome selective breeding method based on machine learning, which comprises the following steps: randomly dividing 132 samples into a training set and a test set according to a ratio of 4: 1, and respectively extracting first 1-100 thousand variation sites as gradient training data sets according to a whole genome association analysis result; and on the basis of 10 classic regression models and the dimensionality-reduced variation data set, machine learning training and evaluation are carried out on 105 training set samples, finally, the variation data set with the highest prediction accuracy and an optimal model are screened out, and phenotype prediction is carried out on 27 test set samples by using the optimal model. The invention relates to the technical field of plant breeding, and the grape downy mildew resistance whole-genome selective breeding method based on machine learning has remarkable advantages in resistance prediction and screening, breeding efficiency improvement and multi-character comprehensive improvement, breaks through the limitation of a traditional breeding method, and is suitable for large-scale popularization and application. An innovative solution is provided for green and sustainable development of the grape industry.

The invention particularly relates to a water-saving irrigation system for intelligent agricultural vegetable seedling raising, and relates to the technical field of intelligent agriculture. And a decision and processing module. According to the method, multi-dimensional data perception and intelligent decision are adopted, so that the core contradiction that disease prevention needs drying and seedling strengthening needs stress in traditional seedling culture is solved; through a non-contact infrared leaf temperature sensor, a miniature resistance-type leaf surface humidity-sensitive sensor and the like, key data such as leaf temperature and leaf surface humidity state are accurately collected, a leaf surface humidity prediction model is constructed, a disease risk window is divided in advance, and propagation of diseases such as downy mildew and gray mold is blocked from the source; the stress intensity is controlled through the wilting coefficient, so that the seedlings are prevented from withering due to excessive stress, and moderate drought is ensured to stimulate root growth.

The invention relates to the field of biocontrol of plant diseases. The invention provides a Streptomyces griseoaurantiacus strain HMQAUfx21024, the strain is preserved in the China General Microbiological Culture Collection Center on January 3, 2025, and the preservation registration number of the strain is CGMCC No. 33290. The invention further provides a preparation method of the strain HMQAUfx21024, and the strain HMQAUfx21024 has the advantages that the strain HMQAUfx21024 can be used for preparing the strain HMQAUfx21024; the invention also provides a biocontrol inoculant. The invention also provides a preparation method of the biocontrol inoculant. The invention also provides application of the strain HMQAUfx21024 or the biocontrol microbial inoculum as described above or the biocontrol microbial inoculum prepared by the preparation method as described above. The invention further provides a method for preventing and treating plant diseases. The biocontrol agent is specially developed for cucumber downy mildew and target leaf spot, due to the fact that the biocontrol agent is a biological agent, a series of problems caused by use of chemical pesticides are completely avoided, pollution-free production of greenhouse vegetables is facilitated, farmers can not use or reduce the use amount of other chemical pesticides, expenses can be saved for the farmers, and the cost is reduced. Vegetable export is facilitated, and the income of farmers can be increased.

The bacillus velezensis MY-8 disclosed by the invention has obvious broad-spectrum antibacterial activity, and has an obvious inhibition effect on various plant pathogenic bacteria including cabbage alternaria alternata, rhizopus arrhizus, cladosporium very fine, fusarium tripteroides, alternaria alternata, Columbia cladosporium, botryosphaeria dothidea, fusarium oxysporum, geotrichum candidum, colletotrichum gloeosporioides and A. arundinis, and the bacillus velezensis MY-8 has an obvious inhibition effect on various plant pathogenic bacteria such as bacillus velezensis MY-8. The compound has a remarkable control effect on downy mildew, is expected to be prepared into a microbial preparation for biological control, and can be used as a biopesticide, a bio-fertilizer, a plant growth promoter and the like. The bacillus velezensis MY-8 disclosed by the invention can also express a structural domain containing protein of DUF4393, so that the bacillus velezensis MY-8 has potential bacteriophage infection resistance.

The invention belongs to the technical field of microbial fertilizers, and particularly relates to a disease and pest resistant microbial fertilizer based on traditional Chinese medicinal materials and a preparation method. The pest and disease resistant microbial fertilizer is prepared from the following raw materials in parts by mass: 15-25 parts of a traditional Chinese medicinal material extract, 70-100 parts of a composite carrier, 2-4 parts of composite microbial flora, 5-10 parts of potassium fulvate, 5-10 parts of sodium humate, 5-10 parts of polyglutamic acid and 15-25 parts of water, the pest and disease resistant microbial fertilizer provided by the invention not only has high viable count, but also has good stability, and can inhibit powdery mildew, downy mildew and aphids of cucumbers at the same time, so that the cucumbers have high acre yield, soluble total sugar content and soluble solid content.

The invention discloses application of a KASP molecular marker in identification of melon downy mildew resistance, and belongs to the technical field of molecular markers. The invention aims to develop the KASP molecular marker closely linked with the melon downy mildew resistance and accelerate the assistant breeding process of the melon downy mildew resistance molecular marker. The KASP marker for identifying the melon downy mildew resistance in the application is CmPcu9.6, and the marker is positioned to a major QTL (quantitative trait loci) related to the melon downy mildew resistance by constructing a high-density genetic map. A group of KASP molecular markers which are closely linked with the melon downy mildew resistance are developed according to a polymorphic SNP (Single Nucleotide Polymorphism) site at chr09: 22824300 in the QTL. The marker can be used for genotype detection of muskmelon downy mildew resistance materials, can successfully distinguish disease-resistant materials and susceptible materials, and can be combined with a high-throughput genotyping detection system to quickly identify downy mildew resistance germplasm resources, accelerate breeding of muskmelon downy mildew resistance varieties and improve breeding efficiency.

The invention discloses bacillus megaterium and application thereof in prevention and treatment of cucumber downy mildew. The bacillus megaterium ZF521 for preventing and treating the cucumber downy mildew, provided by the invention, has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.31036 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. A potted living body test shows that the highest prevention and treatment effect of the bacillus megaterium on the cucumber downy mildew can reach 72.62%, meanwhile, the bacillus megaterium CGMCC No.31036 can promote the increase of the content of three endogenous hormones in the cucumber, and the bacillus megaterium CGMCC No.31036 is applied to the cucumber, so that the cucumber downy mildew can be effectively prevented and treated, and the growth of the cucumber is promoted.

The invention discloses a novel soybean downy mildew resistance molecule detection method. The invention provides a method for predicting soybean downy mildew resistance, which comprises the following steps: 1) acquiring the expression quantity ratio of Pm18s gene to GmActin gene in inoculated leaves of to-be-detected soybean inoculated with downy mildew, and recording the expression quantity ratio as Pm18s / GmActin ratio as sample data; and 2) predicting the downy mildew resistance of the soybean to be detected according to the sample data. The method comprises the following steps of: inoculating downy mildew bacteria on the back of a main leaf by using a smearing method by taking a soybean of which the first main leaf is completely expanded as a material, extracting RNA (Ribonucleic Acid) 8 days after inoculation, measuring the ratio of a downy mildew bacteria Pm18s gene / soybean Actin by semi-quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction), and screening resistant soybeans. The technology not only has the advantages of accurate detection result and no climate influence, but also can greatly shorten the identification period and save land and labor.

This invention relates to the field of pesticide technology, specifically to a fungicidal composition, its preparation method, and its uses. The invention provides a fungicidal composition comprising an active ingredient, a first functional component, a second functional component, and an adjuvant; the active ingredient comprises fluthiazopyrone and cyazofamid; the first functional component comprises at least one selected from silica, titanium dioxide, and zinc oxide; the second functional component comprises at least one selected from citric acid, acetic acid, benzoic acid, urea, and triethanolamine. By introducing specific first and second functional components, this invention synergistically works with the active ingredient, effectively overcoming the key defects of fluthiazopyrone's easy photodegradation and cyazofamid's easy hydrolysis, thus improving the stability of the compound formulation; while ensuring the excellent control efficacy and resistance management advantages of both against oomycete diseases such as grape downy mildew, it extends the duration of action and weather resistance of the agent, providing a more stable, efficient, and long-lasting solution for field application.

The present application relates to a kind of phenyl-tetrazole oxime derivatives and its preparation method and application, wherein the phenyl-tetrazole oxime derivatives have the structure as shown in formula (I), wherein R 1 , R 2 , T, R a , R b , R c , R d , R e Have the meaning described in the present application;The phenyl-tetrazole oxime derivatives described in the present application have excellent prevention effect on cucumber downy mildew, rice sheath blight, cucumber gray mold, cucumber powdery mildew and the like, and can be further developed as a fungicide for application.

The present invention relates to a modified MARCH gene, the wildtype of which comprises SEQ ID No. 1 and SEQ ID No. 3, or comprises a homologous sequence having at least 70% sequence identity to SEQ ID No. 1 and / or SEQ ID No. 3, which modified gene leads to resistance to downy mildew. The modification may leads to an altered gene expression, such as a reduced gene expression. In particular, the modified MARCH gene comprises a modification in SEQ ID No. 3, or in a homologous sequence having at least 70% sequence identity to SEQ ID No. 3. In one embodiment, the modification is on position 77 of SEQ ID No. 3, or on the corresponding position of a homologous sequence having at least 70% sequence identity. In one embodiment, the modified MARCH gene comprises SEQ ID No. 4. The invention further relates to a polynucleotide comprising a modified MARCH gene and a modified Malectin-like-RLK gene, preferably comprising SEQ ID No. 4 and SEQ ID No. 7. The invention further relates to a plant comprising a modified MARCH gene and to a marker for the identification of a modified MARCH gene.

The current invention relates to spinach plants comprising a gene or locus which leads to a broad spectrum resistance to Peronospora effusa (Pe). The invention also relates to progeny of said 5 spinach plants, to propagation material of said spinach plants, to a cell of said spinach plants, to seed of said spinach plants, and to harvested leaves of said spinach plants. The invention also relates to use of said spinach plants in breeding to confer resistance against Peronospora effusa. The invention further relates to a method for generating a spinach plant having a broad spectrum resistance to Peronospora effusa.

The invention relates to the technical field of fertilizers, and discloses charcoal containing a high-concentration sophora flavescens active component and a preparation method and application thereof.The charcoal is prepared by adsorbing the high-concentration sophora flavescens active component through sophora flavescens charcoal, and the sophora flavescens active component comprises sophora flavescens alkaloid and sophora flavescens isopentenyl flavone; after the high-concentration lightyellow sophora root active component charcoal is applied, lightyellow sophora root alkaloid and lightyellow sophora root isopentenyl flavone can be continuously and slowly released, and the slow release time is longer than 40 days. When being applied to commercial crops such as vegetables and tobaccos, the pesticide composition can be used for continuously preventing various diseases and insect pests such as Chinese cabbage downy mildew, flue-cured tobacco aphids, oriental tobacco budworms, bemisia tabaci, tobacco mosaic virus diseases, tobacco tomato spotted wilt virus diseases and tobacco sooty mould in a long-acting manner, and the harm such as water source pollution caused by application of chemical drugs is reduced.

The invention discloses a method for improving botrytis cinerea resistance of plants, and belongs to the technical field of biology. The method comprises the following steps: up-regulating or enhancing or improving the expression of a coding gene of protein in a receptor plant or up-regulating or enhancing or improving the activity and / or content of the protein, so that the resistance of the receptor plant to gray mold is increased, and the amino acid sequence of the protein is SEQ ID NO: 1. Experiments prove that the disease resistance of plants to gray mold can be improved by utilizing the protein SPR7 and the coding gene thereof provided by the invention, and compared with Ailsa Craig plants, the resistance of T1-generation SPR7 transgenic tomato plants to gray mold is greatly improved. Therefore, the protein SPR7 and the coding gene thereof have great theoretical significance and practical value in improving the botrytis cinerea resistance of plants. The invention has important application and market prospects in the field of agriculture.

The present invention discloses a substituted pyrimidine (thio) ester compound, the structure of which is shown in the general formula I: the definition of each substituent in the formula is shown in the specification. The compound of the present invention has excellent fungicidal activity and has excellent control effect on powdery mildew, downy mildew and the like.

The present invention discloses a carbonyl-containing compound, its preparation method and uses. The structure of the compound is as described in General Formula I, and the definitions of each substituent are as described in the specification and claims. The compounds of the present invention have broad-spectrum bactericidal activity against various plant fungal diseases, and can be used alone or in combination with other agents for the control of various plant fungal diseases, especially having excellent control effects on cucumber downy mildew, cucumber anthracnose, wheat powdery mildew, corn rust, rice sheath blight, and cucumber gray mold.

This invention relates to a new and distinct variety of grapevine plant named ‘IVC AN1’. The new variety is primarily adapted to the growing conditions of the temperate or dry areas, e.g. southern Italy, with low rainfall and low disease pressure (namely downy mildew), with winter minimum temperatures down to 10° C., and is characterized by attractive berries with yellowish green color which are uniform in size and shape and are firm with tender crunchy skin.

The invention relates to a sterilization composition containing fluopyram, in particular to application of the sterilization composition containing fluopyram and iprodione to prevention and treatment of cucumber downy mildew, and the composition is mainly characterized in that the weight ratio of fluopyram to iprodione is 1: 1-1: 12, the composition is prepared into a suspending agent, and the suspending agent is a suspending agent. The dosage of the effective active ingredients is 30-120 g / hectare.

The present invention relates to a lettuce plant that is resistant to downy mildew, more specifically to a lettuce plant that comprises a resistance gene that confers resistance to oomycetes in lettuce, more specifically B. lactucae. Furthermore, the present invention relates to a resistance gene and a method for selecting a lettuce plant that is resistant to downy mildew.

The application relates to a pesticide composition containing silicon-rich rings and thiamethoxam and application thereof in prevention and treatment of downy mildew, and belongs to the technical field of agricultural pesticides. In the application, heat-sensitive silicon-rich ring raw medicine is coated with a capsule wall material to form microcapsules, so that the chemical stability of the active ingredient in the storage process is effectively improved. The results of potting test show that the branch number, effective branch number and fruiting number of the pepper plants treated with the pesticide composition are significantly higher than those of the single-dose treatment group, which indicates that the pesticide composition can promote the vegetative growth and reproductive growth of the pepper, help to build a high-yield plant type, and improve the yield potential of crops. Field test data show that the prevention and treatment effect of the pesticide composition on the pepper downy mildew reaches 90.2%, which is significantly better than that of the single-dose treatment group. This indicates that the combination of the silicon-rich rings and thiamethoxam plays a synergistic effect in the prevention and treatment of the pepper downy mildew.

The invention discloses a preparation method of a photocatalyst for removing downy mildew and botrytis cinerea of a carbon-based loaded organic photovoltaic material. The method comprises the following operation steps: A, cleaning coconut shells, and drying until the coconut shells are completely dried; b, crushing the completely dried coconut shells with a crusher, and sieving with a 200-mesh sieve to obtain coconut shell powder; c, drying the coconut shell powder, and performing high-temperature pyrolysis treatment on the dried coconut shell powder in a tubular furnace to obtain modified carbon-based material coconut shell carbon particles; d, mixing an organic photovoltaic material and chloroform according to the following weight ratio: 0.01-0.02 g of the organic photovoltaic material and 10-20 ml of chloroform to obtain an organic photocatalyst mixed solution; and E, uniformly coating modified carbon-based material coconut shell carbon particles with the organic photocatalyst mixed solution obtained in the step D, and drying at 60 DEG C for 24 hours to obtain the coconut shell carbon-based loaded organic photocatalyst. And F, adding the coconut shell carbon-based loaded organic photocatalyst obtained in the step E into a reinforcing agent polyethylene glycol solution, uniformly mixing for 0.5 h, and drying at 60 DEG C for 12 h to obtain the carbon-based loaded organic photovoltaic material photocatalyst for removing grape downy mildew and botrytis cinerea. The photocatalyst obtained by the method can efficiently and quickly remove the plasmopara viticola and the botrytis cinerea at the same time, can be repeatedly utilized, solves the problems of a photocatalyst loading process and cyclic utilization, and provides a real green, environment-friendly and pollution-free treatment method for removing the plasmopara viticola and the botrytis cinerea.

The invention relates to the technical field of grape cultivation, in particular to a method for delaying vitis heyneana germination, which comprises the following steps: in the first ten-day period of March, spraying a treating agent on vitis heyneana, and the treating agent comprises the following components: maleic hydrazide, ethephon, paclobutrazol, morning glory extract and tobacco extract. The method is easy to operate, low in cost, green and safe, germination of vitis quinquangularis is delayed by spraying the pesticide, high-incidence time of downy mildew is avoided in the florescence of the vitis quinquangularis, and meanwhile the growth quality of the vitis quinquangularis is effectively improved.

The present invention relates to the technical field of pesticides, and provides a sterilization composition, the sterilization composition contains active components of pyrazol-ethyl carbamate and amino-oligosaccharin, and the weight percentage of the pyrazol-ethyl carbamate to the amino-oligosaccharin is 1: 10-30: 1. The invention further relates to application of the bactericidal composition to prevention and treatment of cucumber downy mildew and the like.

The present invention discloses a dihydrobenzofuranyl-containing isoxazole compound, a preparation method thereof, and an application thereof. The dihydrobenzofuranyl-containing isoxazole compound is shown in formula (I). The compound or its pharmaceutically acceptable salt has a simple preparation method, high yield, and low cost. The obtained 3-(7-methoxy-2,3-dihydrobenzofuran-5-yl)-5-aryl isoxazole compound or its pharmaceutically acceptable salt exhibits high activity against plant pathogens such as cucumber downy mildew, has good control effects, and can be used as an alternative drug for plant fungicides.

The invention provides an amidine compound containing oxime ether and a preparation method and application thereof.The amidine compound containing oxime ether has the structure shown in the formula I. The amidine compound containing oxime ether has a remarkable effect on prevention and treatment of agricultural and forestry diseases, especially has a good prevention and treatment effect on cucumber powdery mildew and cucumber downy mildew, and is wide in application prospect.

The present invention relates to a lettuce plant that is resistant to downy mildew, more specifically to a lettuce plant that comprises a resistance gene that confers broad spectrum resistance to oomycetes in lettuce, more specifically B. lactucae. Furthermore, the present invention relates to a resistance gene and a method for obtaining a lettuce plant that is resistant to downy mildew, wherein the method comprises the step of introducing said resistance gene into a lettuce plant.

The invention discloses a method for preventing and treating peronophythora litchii, relates to the technical field of peronophythora litchii, and aims to promote disease-resistant varieties and vertical-resistant varieties to control occurrence of peronophythora litchii, the method has a good short-term effect but cannot last for a long time, and the variety with horizontal resistance has relatively stable disease resistance, which is the key point of current breeding. In production, a disease-resistant variety should be selected according to the epidemic degree of peronophythora blight, the sensitive period of the litchi peronophythora blight is a flowering period and a coloring and mature period, and in prevention and treatment, pesticide should be applied in three key periods, namely after flower thinning and shortening, when female flowers bloom and a litchi coloring period; after an orchard is cleaned and fruits are harvested, deadwood on trees and diseased fruits and rotten fruits on the ground are removed in combination with pruning, the fruits are burnt down in a concentrated mode, then a 1% copper sulfate solution is sprayed on soil below tree crowns, and 600-fold liquid of 30% copper oxychloride suspension or 800-fold liquid of 77% Kovalerate wettable powder is sprayed on the tree crowns once.