106 results about "Downy mildew" patented technology

The invention discloses a vegetable disease incubation period detection method and system based on bimodal time sequence collaborative fusion, and the method comprises the steps: obtaining a leaf image through building an acquisition environment, constructing a training sample data set, and constructing a downy mildew incubation period spectral feature adaptive enhancement (AW-FPF) module; the method comprises the following steps: decomposing a hyperspectral signal into low-frequency and high-frequency components through spectrum time sequence adaptive wavelet decoupling, obtaining an enhanced feature tensor through spectrum multi-scale pathological feature frequency-time dual-path aggregation fusion frequency domain and time domain paths, and obtaining a first feature sequence through weighted screening by using a multi-head attention mechanism; a downy mildew incubation period prediction (HyChl-TFNet) model containing a hyperspectral branch, a chlorophyll fluorescence parameter branch, a feature fusion branch and a classifier is constructed, bimodal features are processed and fused to output a day number prediction result, accurate recognition of the downy mildew incubation period is achieved, the detection precision can be controlled to the day number level, and the detection accuracy is improved. And an accurate time basis is provided for early prevention and control of diseases.

The invention relates to the technical field of image recognition, in particular to a horticultural product fungal disease image recognition system and method, and the system comprises an image collection module, an image segmentation module, an anomaly detection module, a feature extraction module, a disease recognition module and a decision output module. In the prior art, the intensity of a light supplementing lamp is manually adjusted, and instantly changing illumination conditions (such as cloud layer shielding and tree shadow shaking) are difficult to deal with in an open-air plantation, so that leaf scab details are often submerged by strong light or dim light is blurred; according to the scheme, a closed-loop linkage system of ambient light perception and an annular LED is innovatively deployed, and the light supplementing intensity is reversely adjusted in a millisecond level by tracking ambient brightness in real time, so that purple gray hyphae of downy mildew in mist in the morning and anthracnose black spots under the noon burning sun present completely consistent color saturation and texture definition in imaging; and the problem of misdiagnosis caused by illumination fluctuation in the traditional method is thoroughly solved.

The invention discloses a grape downy mildew resistance whole genome selective breeding method based on machine learning, which comprises the following steps: randomly dividing 132 samples into a training set and a test set according to a ratio of 4: 1, and respectively extracting first 1-100 thousand variation sites as gradient training data sets according to a whole genome association analysis result; and on the basis of 10 classic regression models and the dimensionality-reduced variation data set, machine learning training and evaluation are carried out on 105 training set samples, finally, the variation data set with the highest prediction accuracy and an optimal model are screened out, and phenotype prediction is carried out on 27 test set samples by using the optimal model. The invention relates to the technical field of plant breeding, and the grape downy mildew resistance whole-genome selective breeding method based on machine learning has remarkable advantages in resistance prediction and screening, breeding efficiency improvement and multi-character comprehensive improvement, breaks through the limitation of a traditional breeding method, and is suitable for large-scale popularization and application. An innovative solution is provided for green and sustainable development of the grape industry.

The invention particularly relates to a water-saving irrigation system for intelligent agricultural vegetable seedling raising, and relates to the technical field of intelligent agriculture. And a decision and processing module. According to the method, multi-dimensional data perception and intelligent decision are adopted, so that the core contradiction that disease prevention needs drying and seedling strengthening needs stress in traditional seedling culture is solved; through a non-contact infrared leaf temperature sensor, a miniature resistance-type leaf surface humidity-sensitive sensor and the like, key data such as leaf temperature and leaf surface humidity state are accurately collected, a leaf surface humidity prediction model is constructed, a disease risk window is divided in advance, and propagation of diseases such as downy mildew and gray mold is blocked from the source; the stress intensity is controlled through the wilting coefficient, so that the seedlings are prevented from withering due to excessive stress, and moderate drought is ensured to stimulate root growth.

The invention discloses application of a KASP molecular marker in identification of melon downy mildew resistance, and belongs to the technical field of molecular markers. The invention aims to develop the KASP molecular marker closely linked with the melon downy mildew resistance and accelerate the assistant breeding process of the melon downy mildew resistance molecular marker. The KASP marker for identifying the melon downy mildew resistance in the application is CmPcu9.6, and the marker is positioned to a major QTL (quantitative trait loci) related to the melon downy mildew resistance by constructing a high-density genetic map. A group of KASP molecular markers which are closely linked with the melon downy mildew resistance are developed according to a polymorphic SNP (Single Nucleotide Polymorphism) site at chr09: 22824300 in the QTL. The marker can be used for genotype detection of muskmelon downy mildew resistance materials, can successfully distinguish disease-resistant materials and susceptible materials, and can be combined with a high-throughput genotyping detection system to quickly identify downy mildew resistance germplasm resources, accelerate breeding of muskmelon downy mildew resistance varieties and improve breeding efficiency.

The invention discloses bacillus megaterium and application thereof in prevention and treatment of cucumber downy mildew. The bacillus megaterium ZF521 for preventing and treating the cucumber downy mildew, provided by the invention, has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.31036 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. A potted living body test shows that the highest prevention and treatment effect of the bacillus megaterium on the cucumber downy mildew can reach 72.62%, meanwhile, the bacillus megaterium CGMCC No.31036 can promote the increase of the content of three endogenous hormones in the cucumber, and the bacillus megaterium CGMCC No.31036 is applied to the cucumber, so that the cucumber downy mildew can be effectively prevented and treated, and the growth of the cucumber is promoted.

The invention discloses a novel soybean downy mildew resistance molecule detection method. The invention provides a method for predicting soybean downy mildew resistance, which comprises the following steps: 1) acquiring the expression quantity ratio of Pm18s gene to GmActin gene in inoculated leaves of to-be-detected soybean inoculated with downy mildew, and recording the expression quantity ratio as Pm18s / GmActin ratio as sample data; and 2) predicting the downy mildew resistance of the soybean to be detected according to the sample data. The method comprises the following steps of: inoculating downy mildew bacteria on the back of a main leaf by using a smearing method by taking a soybean of which the first main leaf is completely expanded as a material, extracting RNA (Ribonucleic Acid) 8 days after inoculation, measuring the ratio of a downy mildew bacteria Pm18s gene / soybean Actin by semi-quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction), and screening resistant soybeans. The technology not only has the advantages of accurate detection result and no climate influence, but also can greatly shorten the identification period and save land and labor.

This invention relates to the field of pesticide technology, specifically to a fungicidal composition, its preparation method, and its uses. The invention provides a fungicidal composition comprising an active ingredient, a first functional component, a second functional component, and an adjuvant; the active ingredient comprises fluthiazopyrone and cyazofamid; the first functional component comprises at least one selected from silica, titanium dioxide, and zinc oxide; the second functional component comprises at least one selected from citric acid, acetic acid, benzoic acid, urea, and triethanolamine. By introducing specific first and second functional components, this invention synergistically works with the active ingredient, effectively overcoming the key defects of fluthiazopyrone's easy photodegradation and cyazofamid's easy hydrolysis, thus improving the stability of the compound formulation; while ensuring the excellent control efficacy and resistance management advantages of both against oomycete diseases such as grape downy mildew, it extends the duration of action and weather resistance of the agent, providing a more stable, efficient, and long-lasting solution for field application.

The present application relates to a kind of phenyl-tetrazole oxime derivatives and its preparation method and application, wherein the phenyl-tetrazole oxime derivatives have the structure as shown in formula (I), wherein R 1 , R 2 , T, R a , R b , R c , R d , R e Have the meaning described in the present application;The phenyl-tetrazole oxime derivatives described in the present application have excellent prevention effect on cucumber downy mildew, rice sheath blight, cucumber gray mold, cucumber powdery mildew and the like, and can be further developed as a fungicide for application.

The present invention relates to a modified MARCH gene, the wildtype of which comprises SEQ ID No. 1 and SEQ ID No. 3, or comprises a homologous sequence having at least 70% sequence identity to SEQ ID No. 1 and / or SEQ ID No. 3, which modified gene leads to resistance to downy mildew. The modification may leads to an altered gene expression, such as a reduced gene expression. In particular, the modified MARCH gene comprises a modification in SEQ ID No. 3, or in a homologous sequence having at least 70% sequence identity to SEQ ID No. 3. In one embodiment, the modification is on position 77 of SEQ ID No. 3, or on the corresponding position of a homologous sequence having at least 70% sequence identity. In one embodiment, the modified MARCH gene comprises SEQ ID No. 4. The invention further relates to a polynucleotide comprising a modified MARCH gene and a modified Malectin-like-RLK gene, preferably comprising SEQ ID No. 4 and SEQ ID No. 7. The invention further relates to a plant comprising a modified MARCH gene and to a marker for the identification of a modified MARCH gene.

The current invention relates to spinach plants comprising a gene or locus which leads to a broad spectrum resistance to Peronospora effusa (Pe). The invention also relates to progeny of said 5 spinach plants, to propagation material of said spinach plants, to a cell of said spinach plants, to seed of said spinach plants, and to harvested leaves of said spinach plants. The invention also relates to use of said spinach plants in breeding to confer resistance against Peronospora effusa. The invention further relates to a method for generating a spinach plant having a broad spectrum resistance to Peronospora effusa.

The invention discloses a method for improving botrytis cinerea resistance of plants, and belongs to the technical field of biology. The method comprises the following steps: up-regulating or enhancing or improving the expression of a coding gene of protein in a receptor plant or up-regulating or enhancing or improving the activity and / or content of the protein, so that the resistance of the receptor plant to gray mold is increased, and the amino acid sequence of the protein is SEQ ID NO: 1. Experiments prove that the disease resistance of plants to gray mold can be improved by utilizing the protein SPR7 and the coding gene thereof provided by the invention, and compared with Ailsa Craig plants, the resistance of T1-generation SPR7 transgenic tomato plants to gray mold is greatly improved. Therefore, the protein SPR7 and the coding gene thereof have great theoretical significance and practical value in improving the botrytis cinerea resistance of plants. The invention has important application and market prospects in the field of agriculture.

This invention relates to a new and distinct variety of grapevine plant named ‘IVC AN1’. The new variety is primarily adapted to the growing conditions of the temperate or dry areas, e.g. southern Italy, with low rainfall and low disease pressure (namely downy mildew), with winter minimum temperatures down to 10° C., and is characterized by attractive berries with yellowish green color which are uniform in size and shape and are firm with tender crunchy skin.

The invention relates to a sterilization composition containing fluopyram, in particular to application of the sterilization composition containing fluopyram and iprodione to prevention and treatment of cucumber downy mildew, and the composition is mainly characterized in that the weight ratio of fluopyram to iprodione is 1: 1-1: 12, the composition is prepared into a suspending agent, and the suspending agent is a suspending agent. The dosage of the effective active ingredients is 30-120 g / hectare.

The present invention relates to a lettuce plant that is resistant to downy mildew, more specifically to a lettuce plant that comprises a resistance gene that confers resistance to oomycetes in lettuce, more specifically B. lactucae. Furthermore, the present invention relates to a resistance gene and a method for selecting a lettuce plant that is resistant to downy mildew.

The application relates to a pesticide composition containing silicon-rich rings and thiamethoxam and application thereof in prevention and treatment of downy mildew, and belongs to the technical field of agricultural pesticides. In the application, heat-sensitive silicon-rich ring raw medicine is coated with a capsule wall material to form microcapsules, so that the chemical stability of the active ingredient in the storage process is effectively improved. The results of potting test show that the branch number, effective branch number and fruiting number of the pepper plants treated with the pesticide composition are significantly higher than those of the single-dose treatment group, which indicates that the pesticide composition can promote the vegetative growth and reproductive growth of the pepper, help to build a high-yield plant type, and improve the yield potential of crops. Field test data show that the prevention and treatment effect of the pesticide composition on the pepper downy mildew reaches 90.2%, which is significantly better than that of the single-dose treatment group. This indicates that the combination of the silicon-rich rings and thiamethoxam plays a synergistic effect in the prevention and treatment of the pepper downy mildew.

The invention relates to the technical field of grape cultivation, in particular to a method for delaying vitis heyneana germination, which comprises the following steps: in the first ten-day period of March, spraying a treating agent on vitis heyneana, and the treating agent comprises the following components: maleic hydrazide, ethephon, paclobutrazol, morning glory extract and tobacco extract. The method is easy to operate, low in cost, green and safe, germination of vitis quinquangularis is delayed by spraying the pesticide, high-incidence time of downy mildew is avoided in the florescence of the vitis quinquangularis, and meanwhile the growth quality of the vitis quinquangularis is effectively improved.

The present invention relates to the technical field of pesticides, and provides a sterilization composition, the sterilization composition contains active components of pyrazol-ethyl carbamate and amino-oligosaccharin, and the weight percentage of the pyrazol-ethyl carbamate to the amino-oligosaccharin is 1: 10-30: 1. The invention further relates to application of the bactericidal composition to prevention and treatment of cucumber downy mildew and the like.

The present invention discloses a dihydrobenzofuranyl-containing isoxazole compound, a preparation method thereof, and an application thereof. The dihydrobenzofuranyl-containing isoxazole compound is shown in formula (I). The compound or its pharmaceutically acceptable salt has a simple preparation method, high yield, and low cost. The obtained 3-(7-methoxy-2,3-dihydrobenzofuran-5-yl)-5-aryl isoxazole compound or its pharmaceutically acceptable salt exhibits high activity against plant pathogens such as cucumber downy mildew, has good control effects, and can be used as an alternative drug for plant fungicides.

The present invention relates to a lettuce plant that is resistant to downy mildew, more specifically to a lettuce plant that comprises a resistance gene that confers broad spectrum resistance to oomycetes in lettuce, more specifically B. lactucae. Furthermore, the present invention relates to a resistance gene and a method for obtaining a lettuce plant that is resistant to downy mildew, wherein the method comprises the step of introducing said resistance gene into a lettuce plant.

The invention discloses a method for preventing and treating peronophythora litchii, relates to the technical field of peronophythora litchii, and aims to promote disease-resistant varieties and vertical-resistant varieties to control occurrence of peronophythora litchii, the method has a good short-term effect but cannot last for a long time, and the variety with horizontal resistance has relatively stable disease resistance, which is the key point of current breeding. In production, a disease-resistant variety should be selected according to the epidemic degree of peronophythora blight, the sensitive period of the litchi peronophythora blight is a flowering period and a coloring and mature period, and in prevention and treatment, pesticide should be applied in three key periods, namely after flower thinning and shortening, when female flowers bloom and a litchi coloring period; after an orchard is cleaned and fruits are harvested, deadwood on trees and diseased fruits and rotten fruits on the ground are removed in combination with pruning, the fruits are burnt down in a concentrated mode, then a 1% copper sulfate solution is sprayed on soil below tree crowns, and 600-fold liquid of 30% copper oxychloride suspension or 800-fold liquid of 77% Kovalerate wettable powder is sprayed on the tree crowns once.

To provide a method for specifically and efficiently detecting buckwheat downy mildew bacteria.SOLUTION: The present invention provides a method for detecting buckwheat downy mildew bacteria (Peronospora ducommeti), by a pair of oligonucleotides including a specific base sequence, and PCR using the same.SELECTED DRAWING: Figure 3

The present invention relates to a composite composition and its application in agriculture. Specifically, the present invention relates to a fungicide composition comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is selected from polyoxin, and the active ingredient B is selected from any one or both of cyfluthrin and epoxiconazole. Experiments have shown that the fungicide composition of the present invention exhibits significant synergistic effects and has excellent control effects on plant diseases, particularly potato late blight, tomato late blight, and cucumber downy mildew.

The utility model discloses a downy mildew spore collecting device which comprises a holding assembly, a collecting assembly, a filtering assembly, a catching assembly and a suction assembly, and the holding assembly is sequentially provided with a filtering bin, a catching bin, an air pump bin and a holding part from front to back; the filtering assembly is installed in the filtering bin, the capturing assembly is installed in the capturing bin, the suction assembly is arranged in the air pump bin, the collecting assembly is installed in front of the filtering assembly, and the suction assembly is electrically connected with the power source. According to the device, the holding assembly, the collecting assembly, the filtering assembly, the capturing assembly and the suction assembly are integrated, so that efficient collection of downy mildew spores is achieved. Due to the innovative structural design, the collecting process is quicker and more convenient, and the collecting efficiency is remarkably improved. According to the device, the combination of the filtering assembly and the capturing assembly is adopted, the collection concentration of sporangium is effectively improved, an additional centrifugal concentration step is not needed, the operation process is simplified, and time and cost are saved.

This invention relates to a new and distinct variety of grapevine plant named ‘IVC AN1’. The new variety is primarily adapted to the growing conditions of the temperate or dry areas, e.g. southern Italy, with low rainfall and low disease pressure (namely downy mildew), with winter minimum temperatures down to 10° C., and is characterized by attractive berries with vivid greenish yellow color which are uniform in size and shape and are firm with tender crunchy skin.

The present disclosure provides Brassica oleracea plants having curds or heads exhibiting increased resistance to downy mildew. Such plants may comprise novel introgressed genomic regions associated with disease resistance from Brassica oleracea MYCOCLP. In certain aspects, compositions, including novel polymorphic markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a disease resistance phenotype are provided.

The present invention relates to methods for identifying and selecting plants resistant to downy mildew and other plant pathogens. Methods for identifying new genes encoding proteins associated with plant resistance to downy mildew and other plant pathogens and uses thereof are provided. The disease resistance genes are useful for producing resistant plants through breeding, transgenic modification, or genome editing.

This invention relates to the field of pesticides, specifically to a fungicide composition for foliar spraying, its preparation method, and application scheme. The active ingredients of the fungicide composition include quinoline copper and phosphite A. Phosphite A is selected from at least one of phosphorous acid, alkali metal salts or alkaline earth metal salts of phosphorous acid, alkylphosphonic acids containing C1-C6 groups of phosphorous acid or alkali metal salts of alkylphosphonic acids, alkaline earth metal salts, and aluminum salts. In the fungicide composition, the weight ratio of quinoline copper to phosphite A is 99:1 to 1:99. The fungicide composition of this invention has high activity against bacterial and fungal diseases occurring in various crops, such as canker and downy mildew, and also exhibits significant synergistic effects. It not only improves the antibacterial or bacteriostatic effect but also broadens the control spectrum. Simultaneously, it can reduce the number of applications and alleviate environmental pressure, thereby achieving the effect of prevention-oriented and integrated pest management.

The invention relates to the technical field of image recognition, and discloses an image-based early recognition method and system for main diseases and insect pests of watermelons, and the method comprises the steps: collecting images of the front surfaces of leaves and unshielded stalks, and recording space-time identifiers; segmenting leaves and stalks, and extracting a vein skeleton and a stalk long axis to generate tangential, normal, axial and circumferential direction fields; in the sub-regions divided in the reference direction, indexes are constructed according to color difference, texture difference and edge difference, adjacent difference is carried out, and suspected regions are screened according to threshold values; fringes are extracted in the suspected area, risk strips are reserved according to the variable coefficient of the adjacent center distance, and feature strips are formed through reinspection in the adjacent area by means of end points and anchor points; and projecting the main vectors of the characteristic strips to a direction field to obtain two directional proportionality coefficients, rechecking downy mildew, powdery mildew and gummy stem blight evidences in combination with a parameter set, and outputting categories or uncertainty. The method has the advantages of being stable and explainable in early-stage small scab detection and typing under the greenhouse fixed imaging condition.

The present application relates to the technical field of biotechnology, and proposes a composition for preventing and treating grape downy mildew, a preparation method and application thereof.The composition for preventing and treating grape downy mildew comprises cyazofamid and flavonoid glycoside extract, and the mass ratio of cyazofamid to flavonoid glycoside extract is 5:150-250.Through the above technical solution, the problem of poor prevention and treatment effect of the existing technology on grape downy mildew is solved.