66 results about "Downy mildew" patented technology

The invention discloses a vegetable disease incubation period detection method and system based on bimodal time sequence collaborative fusion, and the method comprises the steps: obtaining a leaf image through building an acquisition environment, constructing a training sample data set, and constructing a downy mildew incubation period spectral feature adaptive enhancement (AW-FPF) module; the method comprises the following steps: decomposing a hyperspectral signal into low-frequency and high-frequency components through spectrum time sequence adaptive wavelet decoupling, obtaining an enhanced feature tensor through spectrum multi-scale pathological feature frequency-time dual-path aggregation fusion frequency domain and time domain paths, and obtaining a first feature sequence through weighted screening by using a multi-head attention mechanism; a downy mildew incubation period prediction (HyChl-TFNet) model containing a hyperspectral branch, a chlorophyll fluorescence parameter branch, a feature fusion branch and a classifier is constructed, bimodal features are processed and fused to output a day number prediction result, accurate recognition of the downy mildew incubation period is achieved, the detection precision can be controlled to the day number level, and the detection accuracy is improved. And an accurate time basis is provided for early prevention and control of diseases.

The invention relates to the technical field of image recognition, in particular to a horticultural product fungal disease image recognition system and method, and the system comprises an image collection module, an image segmentation module, an anomaly detection module, a feature extraction module, a disease recognition module and a decision output module. In the prior art, the intensity of a light supplementing lamp is manually adjusted, and instantly changing illumination conditions (such as cloud layer shielding and tree shadow shaking) are difficult to deal with in an open-air plantation, so that leaf scab details are often submerged by strong light or dim light is blurred; according to the scheme, a closed-loop linkage system of ambient light perception and an annular LED is innovatively deployed, and the light supplementing intensity is reversely adjusted in a millisecond level by tracking ambient brightness in real time, so that purple gray hyphae of downy mildew in mist in the morning and anthracnose black spots under the noon burning sun present completely consistent color saturation and texture definition in imaging; and the problem of misdiagnosis caused by illumination fluctuation in the traditional method is thoroughly solved.

The invention particularly relates to a water-saving irrigation system for intelligent agricultural vegetable seedling raising, and relates to the technical field of intelligent agriculture. And a decision and processing module. According to the method, multi-dimensional data perception and intelligent decision are adopted, so that the core contradiction that disease prevention needs drying and seedling strengthening needs stress in traditional seedling culture is solved; through a non-contact infrared leaf temperature sensor, a miniature resistance-type leaf surface humidity-sensitive sensor and the like, key data such as leaf temperature and leaf surface humidity state are accurately collected, a leaf surface humidity prediction model is constructed, a disease risk window is divided in advance, and propagation of diseases such as downy mildew and gray mold is blocked from the source; the stress intensity is controlled through the wilting coefficient, so that the seedlings are prevented from withering due to excessive stress, and moderate drought is ensured to stimulate root growth.

The invention discloses bacillus megaterium and application thereof in prevention and treatment of cucumber downy mildew. The bacillus megaterium ZF521 for preventing and treating the cucumber downy mildew, provided by the invention, has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.31036 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. A potted living body test shows that the highest prevention and treatment effect of the bacillus megaterium on the cucumber downy mildew can reach 72.62%, meanwhile, the bacillus megaterium CGMCC No.31036 can promote the increase of the content of three endogenous hormones in the cucumber, and the bacillus megaterium CGMCC No.31036 is applied to the cucumber, so that the cucumber downy mildew can be effectively prevented and treated, and the growth of the cucumber is promoted.

The invention discloses a novel soybean downy mildew resistance molecule detection method. The invention provides a method for predicting soybean downy mildew resistance, which comprises the following steps: 1) acquiring the expression quantity ratio of Pm18s gene to GmActin gene in inoculated leaves of to-be-detected soybean inoculated with downy mildew, and recording the expression quantity ratio as Pm18s / GmActin ratio as sample data; and 2) predicting the downy mildew resistance of the soybean to be detected according to the sample data. The method comprises the following steps of: inoculating downy mildew bacteria on the back of a main leaf by using a smearing method by taking a soybean of which the first main leaf is completely expanded as a material, extracting RNA (Ribonucleic Acid) 8 days after inoculation, measuring the ratio of a downy mildew bacteria Pm18s gene / soybean Actin by semi-quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction), and screening resistant soybeans. The technology not only has the advantages of accurate detection result and no climate influence, but also can greatly shorten the identification period and save land and labor.

This invention relates to the field of pesticide technology, specifically to a fungicidal composition, its preparation method, and its uses. The invention provides a fungicidal composition comprising an active ingredient, a first functional component, a second functional component, and an adjuvant; the active ingredient comprises fluthiazopyrone and cyazofamid; the first functional component comprises at least one selected from silica, titanium dioxide, and zinc oxide; the second functional component comprises at least one selected from citric acid, acetic acid, benzoic acid, urea, and triethanolamine. By introducing specific first and second functional components, this invention synergistically works with the active ingredient, effectively overcoming the key defects of fluthiazopyrone's easy photodegradation and cyazofamid's easy hydrolysis, thus improving the stability of the compound formulation; while ensuring the excellent control efficacy and resistance management advantages of both against oomycete diseases such as grape downy mildew, it extends the duration of action and weather resistance of the agent, providing a more stable, efficient, and long-lasting solution for field application.

The present application relates to a kind of phenyl-tetrazole oxime derivatives and its preparation method and application, wherein the phenyl-tetrazole oxime derivatives have the structure as shown in formula (I), wherein R 1 , R 2 , T, R a , R b , R c , R d , R e Have the meaning described in the present application;The phenyl-tetrazole oxime derivatives described in the present application have excellent prevention effect on cucumber downy mildew, rice sheath blight, cucumber gray mold, cucumber powdery mildew and the like, and can be further developed as a fungicide for application.

The present invention relates to a modified MARCH gene, the wildtype of which comprises SEQ ID No. 1 and SEQ ID No. 3, or comprises a homologous sequence having at least 70% sequence identity to SEQ ID No. 1 and / or SEQ ID No. 3, which modified gene leads to resistance to downy mildew. The modification may leads to an altered gene expression, such as a reduced gene expression. In particular, the modified MARCH gene comprises a modification in SEQ ID No. 3, or in a homologous sequence having at least 70% sequence identity to SEQ ID No. 3. In one embodiment, the modification is on position 77 of SEQ ID No. 3, or on the corresponding position of a homologous sequence having at least 70% sequence identity. In one embodiment, the modified MARCH gene comprises SEQ ID No. 4. The invention further relates to a polynucleotide comprising a modified MARCH gene and a modified Malectin-like-RLK gene, preferably comprising SEQ ID No. 4 and SEQ ID No. 7. The invention further relates to a plant comprising a modified MARCH gene and to a marker for the identification of a modified MARCH gene.

The current invention relates to spinach plants comprising a gene or locus which leads to a broad spectrum resistance to Peronospora effusa (Pe). The invention also relates to progeny of said 5 spinach plants, to propagation material of said spinach plants, to a cell of said spinach plants, to seed of said spinach plants, and to harvested leaves of said spinach plants. The invention also relates to use of said spinach plants in breeding to confer resistance against Peronospora effusa. The invention further relates to a method for generating a spinach plant having a broad spectrum resistance to Peronospora effusa.

The invention discloses a method for improving botrytis cinerea resistance of plants, and belongs to the technical field of biology. The method comprises the following steps: up-regulating or enhancing or improving the expression of a coding gene of protein in a receptor plant or up-regulating or enhancing or improving the activity and / or content of the protein, so that the resistance of the receptor plant to gray mold is increased, and the amino acid sequence of the protein is SEQ ID NO: 1. Experiments prove that the disease resistance of plants to gray mold can be improved by utilizing the protein SPR7 and the coding gene thereof provided by the invention, and compared with Ailsa Craig plants, the resistance of T1-generation SPR7 transgenic tomato plants to gray mold is greatly improved. Therefore, the protein SPR7 and the coding gene thereof have great theoretical significance and practical value in improving the botrytis cinerea resistance of plants. The invention has important application and market prospects in the field of agriculture.

The present invention relates to a lettuce plant that is resistant to downy mildew, more specifically to a lettuce plant that comprises a resistance gene that confers resistance to oomycetes in lettuce, more specifically B. lactucae. Furthermore, the present invention relates to a resistance gene and a method for selecting a lettuce plant that is resistant to downy mildew.

The application relates to a pesticide composition containing silicon-rich rings and thiamethoxam and application thereof in prevention and treatment of downy mildew, and belongs to the technical field of agricultural pesticides. In the application, heat-sensitive silicon-rich ring raw medicine is coated with a capsule wall material to form microcapsules, so that the chemical stability of the active ingredient in the storage process is effectively improved. The results of potting test show that the branch number, effective branch number and fruiting number of the pepper plants treated with the pesticide composition are significantly higher than those of the single-dose treatment group, which indicates that the pesticide composition can promote the vegetative growth and reproductive growth of the pepper, help to build a high-yield plant type, and improve the yield potential of crops. Field test data show that the prevention and treatment effect of the pesticide composition on the pepper downy mildew reaches 90.2%, which is significantly better than that of the single-dose treatment group. This indicates that the combination of the silicon-rich rings and thiamethoxam plays a synergistic effect in the prevention and treatment of the pepper downy mildew.

The invention discloses a method for preventing and treating peronophythora litchii, relates to the technical field of peronophythora litchii, and aims to promote disease-resistant varieties and vertical-resistant varieties to control occurrence of peronophythora litchii, the method has a good short-term effect but cannot last for a long time, and the variety with horizontal resistance has relatively stable disease resistance, which is the key point of current breeding. In production, a disease-resistant variety should be selected according to the epidemic degree of peronophythora blight, the sensitive period of the litchi peronophythora blight is a flowering period and a coloring and mature period, and in prevention and treatment, pesticide should be applied in three key periods, namely after flower thinning and shortening, when female flowers bloom and a litchi coloring period; after an orchard is cleaned and fruits are harvested, deadwood on trees and diseased fruits and rotten fruits on the ground are removed in combination with pruning, the fruits are burnt down in a concentrated mode, then a 1% copper sulfate solution is sprayed on soil below tree crowns, and 600-fold liquid of 30% copper oxychloride suspension or 800-fold liquid of 77% Kovalerate wettable powder is sprayed on the tree crowns once.

To provide a method for specifically and efficiently detecting buckwheat downy mildew bacteria.SOLUTION: The present invention provides a method for detecting buckwheat downy mildew bacteria (Peronospora ducommeti), by a pair of oligonucleotides including a specific base sequence, and PCR using the same.SELECTED DRAWING: Figure 3

The utility model discloses a downy mildew spore collecting device which comprises a holding assembly, a collecting assembly, a filtering assembly, a catching assembly and a suction assembly, and the holding assembly is sequentially provided with a filtering bin, a catching bin, an air pump bin and a holding part from front to back; the filtering assembly is installed in the filtering bin, the capturing assembly is installed in the capturing bin, the suction assembly is arranged in the air pump bin, the collecting assembly is installed in front of the filtering assembly, and the suction assembly is electrically connected with the power source. According to the device, the holding assembly, the collecting assembly, the filtering assembly, the capturing assembly and the suction assembly are integrated, so that efficient collection of downy mildew spores is achieved. Due to the innovative structural design, the collecting process is quicker and more convenient, and the collecting efficiency is remarkably improved. According to the device, the combination of the filtering assembly and the capturing assembly is adopted, the collection concentration of sporangium is effectively improved, an additional centrifugal concentration step is not needed, the operation process is simplified, and time and cost are saved.

This invention relates to a new and distinct variety of grapevine plant named ‘IVC AN1’. The new variety is primarily adapted to the growing conditions of the temperate or dry areas, e.g. southern Italy, with low rainfall and low disease pressure (namely downy mildew), with winter minimum temperatures down to 10° C., and is characterized by attractive berries with vivid greenish yellow color which are uniform in size and shape and are firm with tender crunchy skin.

The present disclosure provides Brassica oleracea plants having curds or heads exhibiting increased resistance to downy mildew. Such plants may comprise novel introgressed genomic regions associated with disease resistance from Brassica oleracea MYCOCLP. In certain aspects, compositions, including novel polymorphic markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a disease resistance phenotype are provided.

The present invention relates to methods for identifying and selecting plants resistant to downy mildew and other plant pathogens. Methods for identifying new genes encoding proteins associated with plant resistance to downy mildew and other plant pathogens and uses thereof are provided. The disease resistance genes are useful for producing resistant plants through breeding, transgenic modification, or genome editing.

This invention relates to the field of pesticides, specifically to a fungicide composition for foliar spraying, its preparation method, and application scheme. The active ingredients of the fungicide composition include quinoline copper and phosphite A. Phosphite A is selected from at least one of phosphorous acid, alkali metal salts or alkaline earth metal salts of phosphorous acid, alkylphosphonic acids containing C1-C6 groups of phosphorous acid or alkali metal salts of alkylphosphonic acids, alkaline earth metal salts, and aluminum salts. In the fungicide composition, the weight ratio of quinoline copper to phosphite A is 99:1 to 1:99. The fungicide composition of this invention has high activity against bacterial and fungal diseases occurring in various crops, such as canker and downy mildew, and also exhibits significant synergistic effects. It not only improves the antibacterial or bacteriostatic effect but also broadens the control spectrum. Simultaneously, it can reduce the number of applications and alleviate environmental pressure, thereby achieving the effect of prevention-oriented and integrated pest management.

The invention relates to the technical field of image recognition, and discloses an image-based early recognition method and system for main diseases and insect pests of watermelons, and the method comprises the steps: collecting images of the front surfaces of leaves and unshielded stalks, and recording space-time identifiers; segmenting leaves and stalks, and extracting a vein skeleton and a stalk long axis to generate tangential, normal, axial and circumferential direction fields; in the sub-regions divided in the reference direction, indexes are constructed according to color difference, texture difference and edge difference, adjacent difference is carried out, and suspected regions are screened according to threshold values; fringes are extracted in the suspected area, risk strips are reserved according to the variable coefficient of the adjacent center distance, and feature strips are formed through reinspection in the adjacent area by means of end points and anchor points; and projecting the main vectors of the characteristic strips to a direction field to obtain two directional proportionality coefficients, rechecking downy mildew, powdery mildew and gummy stem blight evidences in combination with a parameter set, and outputting categories or uncertainty. The method has the advantages of being stable and explainable in early-stage small scab detection and typing under the greenhouse fixed imaging condition.

The present application relates to the technical field of biotechnology, and proposes a composition for preventing and treating grape downy mildew, a preparation method and application thereof.The composition for preventing and treating grape downy mildew comprises cyazofamid and flavonoid glycoside extract, and the mass ratio of cyazofamid to flavonoid glycoside extract is 5:150-250.Through the above technical solution, the problem of poor prevention and treatment effect of the existing technology on grape downy mildew is solved.

The application discloses a bacillus thuringiensis and application thereof. The bacillus thuringiensis ZF505 provided by the application has a preservation number of CGMCC No.24585 in the China General Microbiological Culture Collection Center. A potting living body test shows that the bacillus thuringiensis can prevent and treat cucumber downy mildew with a highest effect of 73.01%, and the bacillus thuringiensis CGMCC No.24585 can promote the increase of the content of three endogenous hormones of cucumber, wherein gibberellin is increased by 15.61%, indole acetic acid is increased by 5.05%, and zeatin is increased by 5.12%.

The invention belongs to the technical field of microorganisms, and particularly relates to grape endophytic fungi NZ1-b-1 and application thereof in prevention and treatment of grape diseases. The grape endophytic fungus NZ1-b-1 is classified and named as subclavian endophytic fungus and is preserved in the China Center for Type Culture Collection on July 14, 2025, and the preservation number of the grape endophytic fungus NZ1-b-1 is CCTCC (China Center for Type Culture Collection) NO: M 20251604. The grape endophytic fungi NZ1-b-1 provided by the invention are endophytic fungi separated from grape leaves, the culture method is simple, the cost is low, and the separation method is easy to operate. The grape endophytic fungus NZ1-b-1 has an antibacterial effect on grape botrytis cinerea and white rot fungi, has a relatively high prevention and treatment effect on grape gray mold and white rot, has a certain prevention and treatment effect on grape downy mildew, and has the potential of being developed into a broad-spectrum antibacterial agent for grape fungal diseases.

The present application discloses a pesticide bactericidal composition, a water dispersible granule and a preparation method and a use thereof, and relates to the field of pesticides. The active ingredients of the pesticide bactericidal composition comprise chlorothalonil, tebuconazole and picoxystrobin with a weight ratio of (10-50):(1-2):1, optionally (10-20):(1-6):(1-3), optionally (10-20):(1-2):1 or 10:6:3. The ternary compounded composition of the present application has a synergistic effect in the aspect of preventing and controlling plant diseases, and can achieve above 90% of prevention and control effect of grape downy mildew and wheat powdery mildew. The ratios of the composition in the present application can allow pesticides to better exert a synergistic effect, thereby reducing the usage amount of the pesticides and improving the efficacy.

The invention discloses a hymexazol and sodium trichloroisocyanurate compounded bactericidal composition, which takes hymexazol and sodium trichloroisocyanurate as main active ingredients, and the mass ratio of hymexazol to sodium trichloroisocyanurate is (1: 1)-(200: 1); the bactericidal composition further comprises 1, 3-propane sultone; the ratio of the total amount of hymexazol and sodium trichloroisocyanurate to the mass of 1, 3-propane sultone is (30: 1)-(90: 1). The invention also discloses application of the bactericidal composition in prevention and treatment of downy mildew, fusarium wilt, damping off and black spot of plants. Compared with a single agent of hymexazol, the bactericidal composition provided by the invention can improve the problem that hymexazol is easy to generate resistance, and widens the application range of the agent. The bactericide composition can replace some high-toxicity bactericides, the dosage is reduced, the environment is protected, and meanwhile pesticide residues are reduced. According to the invention, 1, 3-propane sultone is used as a synergist, so that the pesticide effect is enhanced.

The application discloses a grape powdery mildew adaptive recognition method based on boundary sample quantile, relates to the technical field of agricultural lesion recognition, and comprises the following steps: acquiring a grape leaf original image, mapping and converting the grape leaf original image into a leaf grayscale image through a grayscale mapping method; performing boundary recognition on the leaf grayscale image to obtain leaf lesion boundary information; determining a lesion threshold of the leaf lesion boundary information based on a sample quantile extraction method; determining pixels less than the lesion threshold as lesions according to the lesion threshold of each pixel point of the leaf grayscale image, and obtaining an image lesion recognition result; and calculating a lesion proportion of the image lesion recognition result based on a statistical method. The application solves the technical problems of slow lesion recognition speed and low recognition accuracy of grape powdery mildew in the prior art, and achieves the technical effects of improving the lesion recognition speed and recognition accuracy of grape powdery mildew.

The invention discloses a method and a system for constructing a cucumber leaf health state detection model, relates to the technical field of agricultural intellectualization, and solves the problems that a compact and high-discrimination feature set is difficult to form in an existing cucumber leaf health state detection technology, and information is mutually counteracted or noise is superposed after feature fusion. And the cucumber leaf health state identification accuracy is low. The most representative features are obtained through extraction and screening of multiple texture features and color indexes, then a heterogeneous multi-modal fusion model is constructed to effectively process multi-modal data including texture images and color index images, and then a cucumber leaf health state detection model is obtained. The model can accurately detect the health / illness state of cucumber leaves. The method is suitable for the field of cucumber leaf health, downy mildew and powdery mildew detection.

The invention discloses a Burkholderia polluting strain LZ816 and an application of the Burkholderia polluting strain LZ816 in the aspect of preventing and treating litchi diseases, and particularly discloses a Burkholderia polluting strain LZ816 and an application of the Burkholderia polluting strain LZ816. The polluted burkholderia sp. LZ816 is preserved in Guangdong Microbial Culture Collection Center on March 4, 2025, and the preservation number is GDMCC No: 65968. The bacterium and a fermentation product of the bacterium have relatively good bacteriostatic activity on peronophythora litchii and colletotrichum frutescens, and can be used for preventing and treating common diseases of litchi, such as peronophythora litchii, anthracnose of litchi and the like. The bacterium can be used for preventing and treating common diseases of litchi, such as peronophythora litchii and colletotrichum frutescens. The burkholderia sp. LZ816 disclosed by the invention can be used for preparing a novel, efficient and broad-spectrum biocontrol microbial inoculum, and the burkholderia sp. Is high in propagation speed, low in requirement on nutrition, high in colonization capability and high in application value.

The invention relates to the technical field of plant antibacterial agents, in particular to a sorbic hydrazide compound, a preparation method and application. The sorbic hydrazide compound is a compound as shown in a formula I or a formula II, or an isotope derivative of the sorbic hydrazide compound and a pesticide acceptable salt. The sorbic hydrazide compound has broad-spectrum inhibitory activity on phytopathogens such as plant pathogenic fungi, and has excellent inhibitory activity on more than ten kinds of plant pathogenic fungi, especially on oomycetes and ascomycetes pathogenic fungi such as downy mildew bacteria and powdery mildew bacteria. The sorbic hydrazide compound has the advantages of easily available raw materials, simple preparation, low cost and no toxicity to animals and plants, and can be used as an effective component or a synergistic component for development and application of a novel green efficient agricultural antibacterial agent.

The invention provides two compounds for plant induced resistance. The two compounds are respectively 2-(4-chlorphenyl)-2-oxoethyl 1-benzofuran-2-carboxylic ester and 3-methoxy-N-(1-(2-methoxy ethyl)-1, 2, 3, 4-tetrahydroquinoline-7-yl) benzamide, and the two compounds can be used as plant resistance inducers to improve the resistance to rice blast, rice bacterial blight and Chinese cabbage downy mildew.