127 results about "Oomycete" patented technology

The invention relates to bacillus velezensis SMB1 and application thereof in prevention and treatment of plant diseases, and belongs to the technical field of biological prevention and treatment of plant diseases. The strain is preserved in the China Center for Type Culture Collection on November 27, 2024, the preservation address is Luojia mountain, Luojia mountain, eight road, Wuchang District, Wuhan City, Hubei Province, and the preservation number is CCTCC NO: M20242647. The bacillus velezensis SMB1 provided by the invention has a remarkable bacteriostatic effect on plant pathogenic fungi, plant pathogenic oomycetes, plant pathogenic bacteria and animal pathogenic bacteria, has an extremely good broad-spectrum bacteriostatic function, has a great application potential in biological control of plant diseases, can reduce the use of chemical pesticides, and has a good application prospect. The pesticide residue in agricultural products is reduced, the environment is protected, and the urgent demand on pollution-free agricultural product production at present is met.

The invention discloses a phytophthora plant pathogenic oomycete histone lysine methyl transfer protein as well as a coding gene and application thereof. Wherein the phytophthora sojae histone lysine methyltransferase KMT3-1 is as shown in a sequence 2 in the specification, and the phytophthora sojae histone lysine methyltransferase KMT3-1 is as shown in a sequence 2 in the specification; the coding gene is shown as a sequence 1. Experiments prove that the KMT3-1 protein plays an important role in the growth and development process of phytophthora sojae, specifically, after the protein is deleted, the growth rate of phytophthora hyphae is slowed down, the sporangium yield is remarkably reduced, the release of zoospores is stopped, the oospore yield is reduced, the pathogenicity is reduced, and the like. Corresponding histone methyltransferase inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora sojae, phytophthora capsici and phytophthora infestans. The gene provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed on the basis of the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.

The invention discloses arginine methyltransferase protein from phytophthora plant pathogenic oomycetes as well as a coding gene and application thereof. The arginine methyltransferase protein provided by the invention is as shown in sequences 6-8; the coding gene is shown in sequences 1-3. Experiments prove that the protein disclosed by the invention plays an important role in growth, development and pathopoiesis processes of typical phytophthora plant pathogen oomycete phytophthora capsici, and after the protein is deleted or the expression level is reduced, the growth rate of phytophthora capsici hyphae is reduced, the sporangium yield is reduced, the number of zoospore is reduced, the pathogenicity is reduced and the like. Corresponding arginine methyltransferase inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora capsici, phytophthora sojae and phytophthora infestans. The gene provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed based on the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.

The invention discloses a polypeptide ligand SR-22 of a targeted pathogen oomycete membrane protein and an application of the polypeptide ligand SR-22. The amino acid sequence of the polypeptide ligand SR-22 is SRITRLLRRGSGSGSSRITRLLR. The polypeptide ligand SR-22 provided by the invention can remarkably inhibit the growth of various crop pathogenic oomycetes, can well inhibit pathogenic bacteria infection and prevent diseases in practical application, can be subsequently developed into a novel targeted polypeptide pesticide, and is used for green prevention and control of crop oomycetes diseases.

The invention discloses a multifunctional peoriae paenibacillus and application thereof, and relates to the technical field of microorganisms, and the key point of the technical scheme is as follows: Paenibacillus peoriae S6330 is separated from rhizosphere soil; the peoriae paenibacillus has a good inhibition effect on pathogenic bacteria, pathogenic fungi and oomycetes; meanwhile, the peoriae paenibacillus has the functions of dissolving potassium, producing protease, producing amylase, producing cellulase, producing siderophore and the like, has a growth promoting effect on various crops such as wheat, corn, watermelons, small oilseed rapes, tobaccos and the like, and can remarkably improve indexes such as chlorophyll content, plant height, fresh weight, overground part weight, underground part weight and the like of plants; in addition, the peoriae paenibacillus can be used for effectively inhibiting the tobacco bacterial wilt; the peoriae paenibacillus has no environmental pollution problem, is simple in culture condition, and has wide disease prevention and growth promotion application prospects in agricultural production.

The invention discloses preparation and application of a multifunctional microbial preparation for rhizosphere conservation, and relates to the technical field of microorganisms, and the technical key points are as follows: the microbial agent is prepared by mixing bacillus safensis S124, bacillus velezensis S719 and pseudomonas chlororaphis S1130 according to the proportion of 1: 1: 1, and the strain preservation numbers of the microbial agent are CGMCC (China General Microbiological Culture Collection Center) No.29817, CGMCC No.29818 and CGMCC No.29819 in sequence; the microbial preparation can inhibit pathogenic bacteria, plant pathogenic fungi and oomycetes, can promote crop growth, is free of drug resistance, safe and non-toxic, has the effects of preventing diseases and promoting growth, and can provide a biological control method for effective yield increase, weight reduction and pesticide reduction of crops.

The invention discloses an ADF protein from phytophthora as well as a coding gene and application thereof. According to the present invention, the PsADF protein provided by the present invention is the protein represented by sequences 2 and 5 in Phytophthora sojae (Phytophthora sojae); the coding gene is shown as a sequence 3 and a sequence 4. Tests prove that the protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, specifically, after the 64-site mutation of the key amino acid functional site of the protein, the sporangium abnormality and zoospore quantity of the phytophthora sojae are reduced, and the pathogenicity is reduced. The protein provided by the invention has very high application value in control of epidemic disease occurrence and epidemic caused by phytophthora, and provides a molecular target for research and development of novel bactericides in the future. The corresponding ADF inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora sojae, phytophthora capsici and phytophthora infestans. The protein provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed based on the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.

The invention discloses tobacco bacterial wilt biocontrol burkholderia and application thereof, relates to the technical field of agricultural microorganisms, and is technically characterized in that the burkholderia S8288 is preserved in China General Microbiological Culture Collection Center (CGMCC) in October 2024, the preservation number of the burkholderia S8288 is CGMCC NO.32293, the preservation number of the burkholderia S8288 is CGMCC NO.32293, and the preservation number of the burkholderia S8288 is CGMCC NO.32293. The 16S rRNA (ribosomal Ribonucleic Acid) gene sequence of the Burkholderia sp. S8288 strain is as shown in SEQ ID No. 1. The strain has a good inhibition effect on pathogenic fine diseases and oomycetes such as Coreiella solani causing tobacco bacterial wilt and phytophthora nicotianae causing tobacco black shank, and the prevention effect on the tobacco bacterial wilt reaches 50%. The strain can induce death of plant cells and stimulate expression of plant-related immune genes. Besides, the Burkholderia sp. S8288 can decompose potassium, hydrolyze inorganic phosphorus and organic phosphorus and produce protease and NH3, and has potential capabilities of improving soil nutrition and promoting crop growth, and greenhouse tests show that the strain can significantly promote tobacco growth and development.

The invention belongs to the field of gene engineering, and discloses a pythium ultimum PuRWP1 gene and application thereof as a molecular target in prevention and treatment of diseases caused by pythium ultimum. The invention provides a gene PuRWP1 which is from pythium ultimum and is used for controlling a sexual development stage and oospore formation, the gene is composed of 3026 nucleotides, a protein coded by the PuRWP1 gene is composed of 217 amino acids, and knockout of the PuRWP1 gene can lead to remarkable reduction of sporulation ability of a mutant and reduction of spore activity, so that the mutant can be used for preparing a new mutant. The gene can be used as a molecular target to be applied to disease control of oomycetes. The function of the pythium ultimum PuRWP1 gene is verified, and a new direction is provided for further utilizing the gene to regulate and control the physiological functions of oomycetes such as pythium ultimum and preventing and treating oomycetes diseases.

The invention relates to a sterilization liquid agent based on cuprous hydroxide, and also relates to a preparation method and a use method thereof, the suspension of cuprous hydroxide is used as the sterilization liquid agent, and diseases caused by various oomycetes, fungi and / or bacteria can be effectively prevented and treated; wherein the turbid liquid of cuprous hydroxide is obtained by stirring a cuprous chloride coordination compound in a solution state and decomposing the cuprous chloride coordination compound in water. According to the sterilization liquid, the single-time copper dosage is far lower than that of an existing copper ion sterilization agent, and meanwhile the prevention and treatment range of diseases is wider. Therefore, the risk of excessive heavy metal residue caused by the existing copper preparation is solved, and the problem that multiple bactericides need to be used at the same time for preventing and treating one or more diseases is also avoided. The bactericidal liquid provided by the invention has important significance for preventing and treating plant diseases, and has huge application potential in the agricultural market.

The invention discloses an endophytic subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. The strain can inhibit the growth of different physiological races of phytophthora infestans, and has inhibitory activity on various pathogenic oomycetes. The fermentation liquor of the strain contains micromolecular antibacterial substances with relatively high stability, and has remarkable antibacterial activity; protein components in fermentation liquor can induce plants to generate resistance, and infection of phytophthora infestans is effectively inhibited. Different types of microbial agents prepared by utilizing the strain DS1 can be used for effectively preventing and treating potato late blight, and the microbial preparation is used for seed dressing treatment of potatoes and wheat and can be used for remarkably promoting the growth of crops.

The invention provides a botanical fungicide of a chaulmoogra seed extract, which takes sub-chaulmoogra seed oleic acid as a marker, and application of the botanical fungicide in preparation of medicines for preventing and treating plant diseases caused by pathogenic fungi and oomycetes. The hydnocarpus anthelmintica extract is obtained by extracting hydnocarpus anthelmintica seeds with a plurality of different types of organic solvents such as ethanol, petroleum ether and the like and treating the seeds by a physical method, and the hydnocarpus anthelmintica extract contains sub-hydnocarpus anthelmintica oleic acid as markers in different proportions. The extract has the effects of inhibiting conidium germination, bud tube growth or appressorium formation of fungi and oomycetes, effectively preventing pathogenic bacteria from infecting host plants and preventing and treating important plant diseases such as rice blast, mango anthracnose, potato late blight and rice false smut.

The invention provides a pyrroline diketone compound as well as a preparation method and a bactericide application thereof. The name of the pyrroline diketone compound is 1-(2, 6-diethyl-4-methylphenyl)-1H-pyrrole-2, 5-diketone, and the structural formula of the pyrroline diketone compound is shown as a formula I. The pyrroline diketone compound and an agricultural bactericide taking the compound as an effective component are used for preventing and treating various plant diseases caused by plant pathogenic fungi, oomycetes and bacteria. The compound is novel in structure and action mechanism, convenient in synthesis method and good in safety, has broad-spectrum and efficient antibacterial activity, does not have cross resistance with other existing bactericides, can be used for preventing and treating plant diseases of vegetables, fruits, food crops, oil crops, commercial crops, garden crops, traditional Chinese medicinal materials and the like, and has broad application prospects. And particularly, the composition has good chemical protection effect and systemic treatment effect on drug-resistant crop diseases.

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The present application relates to a kind of bactericidal liquid agent based on cuprous hydroxide, and its preparation method and use method, with the suspension of cuprous hydroxide as bactericidal liquid agent, can effectively prevent and cure the disease caused by a variety of oomycetes, fungi and / or bacteria, wherein the suspension of cuprous hydroxide is obtained by stirring in water by the solution state cuprous chloride coordination compound decomposition.This bactericidal liquid agent of the present application can be far lower than the existing copper ion bactericide while the single copper dosage is lower, and the prevention and cure range of disease is wider.This not only solves the risk of heavy metal excess residue caused by the existing copper preparation, but also avoids the problem of needing to use multiple bactericides simultaneously to prevent and cure one or more diseases.The bactericidal liquid agent of the present application has important significance for plant disease prevention and cure, and has great application potential in agricultural market.

The invention provides a preparation method of a microzyme-activated fruit and vegetable by-product biological agent, which comprises the following steps: uniformly mixing a fruit and vegetable by-product powder suspension with the concentration of 1-1.5 g / mL with a nutrient aqueous solution to prepare a fruit and vegetable by-product powder-based culture medium; activating saccharomycetes, inoculating the saccharomycetes to the fruit and vegetable byproduct powder-based culture medium, incubating, fermenting, collecting fermentation liquor, and preparing into powder, so as to obtain the fruit and vegetable byproduct powder-based culture medium. The biological agent prepared by fermenting the fruit and vegetable by-product powder through the saccharomycetes shows broad-spectrum antifungal activity on various plant pathogenic fungi and oomycetes. The biological agent disclosed by the invention shows strong inhibitory activity on various pathogenic bacteria in an in-vitro test. The biological agent provided by the invention provides an environment-friendly and non-toxic alternative scheme, and is used for reducing or replacing the application of the traditional synthetic chemical bactericide in the postharvest treatment of the citrus fruits. This contributes to mitigating potential risks of chemical fungicide residues to the environment and consumer health.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The present invention relates to novel antimicrobial peptides for use as a viable and sustainable alternative to fungicides currently used in agriculture against infection by oomycetes infection, and related methods for protecting crops.

The present invention belongs to the technical field of pesticide sterilization and discloses a fungicide composition containing mandipropamid. The fungicide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is mandipropamid and active ingredient B is either fluoxapiprolin or flufenoxadiazam, with a mass ratio of active ingredient A to active ingredient B of 1:50 to 30:1. The fungicide composition of the present invention has a significant synergistic effect on diseases caused by plant oomycete pathogens, reduces the application amount and frequency of chemical pesticides, improves application efficiency, reduces agricultural production costs, slows the development and development of drug resistance in pathogens, reduces pesticide residues, protects the farmland ecological environment, and ensures the food safety of agricultural products.

The present invention relates to a lettuce plant that is resistant to downy mildew, more specifically to a lettuce plant that comprises a resistance gene that confers resistance to oomycetes in lettuce, more specifically B. lactucae. Furthermore, the present invention relates to a resistance gene and a method for selecting a lettuce plant that is resistant to downy mildew.

The invention relates to a strain of biocontrol and growth-promoting brevibacillus brevis LJQJ21 and an application of the strain of biocontrol and growth-promoting brevibacillus brevis LJQJ21 in prevention and treatment of plant oomycetes diseases, and belongs to the field of research and development of microbial pesticides. The Brevibacillus formis LJQJ21 has the capabilities of promoting growth and preventing diseases, has a relatively strong inhibition effect on mycelial growth of various pathogenic oomycetes (phytophthora capsici, phytophthora infestans, peronophythora litchii and pythium aphanidermatum) causing crop diseases, and can secrete pentapeptide antibacterial substances Edeine A and B, and the Edeine can remarkably inhibit germination of zoospores of the phytophthora capsici. In addition, the microbial agent taking the LJQJ21 spore fermentation bacterial liquid as a main active component has a relatively good prevention and treatment effect on fruit rot caused by phytophthora capsici. The Brevibacillus forts LJQJ21 has obvious growth promoting and disease preventing effects, is safe to human and livestock and environment-friendly, and has potential field application value and development prospect.

The present invention relates to pyridazine-4-yl oxadiazine compounds and their use for controlling harmful microorganisms such as phytopathogenic oomycetes and fungi.

The invention concerns a method for prophylaxis of infections by fungi, particularly by oomycetes, and of bacterial infections in crop and ornamental plants. Areas of application are vine, fruit, vegetable and ornamental plant growing. The method of the invention is characterized in that an aqueous solution of a protease, alone or in combination with β-glucanases and / or chitinases, is prepared, stabilizers, stickers and wetters are added, and the additized solution is applied by conventional techniques to the plants a number of times within the vegetation period, preferably ahead of weather-related phases of high infection threat.

The invention relates to an oxazole compound as shown in a formula (I), a preparation method thereof and application of the oxazole compound in prevention and treatment of plant diseases. Particularly relates to an oxazole compound capable of inhibiting formation of fungus and oomycete appressorium, a preparation method of the oxazole compound and application of the oxazole compound to prevention and treatment of plant diseases caused by fungi and oomycetes.

The present invention relates to a lettuce plant that is resistant to downy mildew, more specifically to a lettuce plant that comprises a resistance gene that confers broad spectrum resistance to oomycetes in lettuce, more specifically B. lactucae. Furthermore, the present invention relates to a resistance gene and a method for obtaining a lettuce plant that is resistant to downy mildew, wherein the method comprises the step of introducing said resistance gene into a lettuce plant.

The invention relates to a method of controlling pests, said method comprising: providing a composition comprising valifenalate and cyazofamid, and applying said composition to a crop plant, a pest population associated with a crop plant, a habitat of a crop plant, or a combination thereof, wherein said valifenalate and said cyazofamid are present in said composition according to a ratio of between 10:1 and 10 1:5, and wherein said pest population comprises at least one organism chosen from the group of oomycetes. The present invention also concerns a method of improving plant resistance against pests and / or inducing plant defense pathways.

The invention provides a greenhouse vegetable organic coupling cultivation method and application. The method comprises the steps that square ditches are dug one month before greenhouse vegetable seedlings are transplanted, corn straw, Penicillium cyclopium 455-1 bacterial suspension, viral vaccines, peanut cakes, wheat bran, farmyard manure, bacterial manure, compound fertilizer and water are sequentially added, then the square ditches are flatly covered with soil and are covered and sealed with a plastic film, and then the greenhouse vegetable seedlings are transplanted. After one month, punching holes in the surface of the mulching film to transplant vegetable seedlings. According to the greenhouse vegetable 455-1 mutant strain fermentation liquor, corn straw and organic fertilizer organic coupling cultivation technology, the growth vigor, root development and photosynthetic efficiency of greenhouse tomatoes, peppers and cucumbers and the resistance to soil-borne oomycetes diseases are remarkably improved.

Compositions comprising Methylobacterium that inhibit infection by a oomycete or fungal plant pathogen and / or reduce severity of symptoms caused by a plant pathogenic oomycete or fungus, methods of making the compositions, and methods of treating plants to inhibit and / or reduce symptoms of oomycete and / or fungal infections are provided.

The application belongs to the technical field of agricultural microorganisms, and particularly relates to a plant endophytic Paenibacillus polymyxa and application thereof in preventing and treating plant diseases. Paenibacillus polymyxa The plant endophytic Paenibacillus polymyxa has the strain number PP8, the classification name Paenibacillus polymyxa, the preservation number CGMCC No. 36638, and is preserved in the China General Microbiological Culture Collection Center on November 17, 2025. The Paenibacillus polymyxa has broad-spectrum inhibitory activity on pathogenic fungi, oomycetes and bacteria, and can produce significant inhibitory effect on plant pathogenic microorganisms through multiple action mechanisms. In addition, the Paenibacillus polymyxa PP8 has the ability to colonize in the internal tissue of plant roots, can exist in the plant body and play the disease-inhibiting effect, thereby further enhancing the prevention and control effect on soil-borne diseases and improving the persistence and stability of the biological control effect.

The application provides a pyrroline dione compound and a preparation method and fungicide application thereof. The pyrroline dione compound is named 1-(2,6-diethyl-4-methylphenyl)-1H-pyrrole-2,5-dione, and a structural formula is shown as formula I: the pyrroline dione compound and an agricultural fungicide with the compound as an effective component are used for preventing and treating various plant diseases caused by plant pathogenic fungi, oomycetes and bacteria. The compound is novel in structure and action mechanism, convenient in synthesis method, good in safety, has broad-spectrum and high-efficiency antibacterial activity, has no cross resistance with other existing fungicides, can be used for preventing and treating plant diseases of vegetables, fruits, food crops, oil crops, economic crops, garden crops and Chinese medicinal materials, and has good chemical protection and internal absorption treatment effects on resistant crop diseases.