86 results about "Pyrroline" patented technology

The present invention refers to an antagonist of imidazoline-1 receptor (I1R) for use in the prevention and / or treatment of an autoinflammatory or autoimmune disease in a human subject, selected from the group consisting of (±)-2-endo-amino-3-exo- isopropylbicyclo[2.2.1]heptane (synonym: AGN192403), N-benzylguanidine, 2-(2- Fluoro-5-methylphenyl)-4,5-dihydro-1H-imidazole (synonym: S23757), 2-(2-chloro-4-iodo-phenylamino)-5-methyl-pyrroline (synonym: LNP911), 1-(4, 5-dihydro-1H-imidazol- 2-yl)isoquinoline (synonym: BU98008), 5-[2-bromophenoxy)methyl]-4,5-dihydro-1,3- oxazol-2-amine (synonym: S23515), 6-methylamino-2methylheptene (synonym: isometheptene), and 4-chloro-2,3-dihydro-2-oxo-1,3-benzothiazol-3-ylacetic acid (synonym: benazolin). Also, to the use of Imidazole propionate in the diagnosis of an autoinflammatory or autoimmune disease in a subject, and further to a method of diagnosis of atherosclerosis comprising assessing the level of ImP in a blood sample and determining whether said level is above or below a value of 41nM.

The invention discloses an application of a KASP molecular marker linked with a pumpkin flavor substance 2-acetyl-1-pyrroline in detection or screening of pumpkin germplasm resources containing 2-acetyl-1-pyrroline, the KASP molecular marker is located at the 712637 site of a chromosome 10 of a Chinese pumpkin genome, and the basic group at the site is T or C. The KASP molecular marker can be used for detecting or screening pumpkin germplasm resources containing 2-acetyl-1-pyrroline. The KASP molecular marker and the amplification primer group thereof can be used for accurately detecting whether the pumpkin germplasm resource contains the aromatic substance 2-acetyl-1-pyrroline or not, so that the judgment efficiency of the aromatic substance can be effectively improved, and the planting manpower, material resources and financial resources of non-target breeding resources are reduced.

The invention relates to the technical field of plant genetic engineering, in particular to application of a transcription factor OsMPS in increasing the content of rice 2-acetyl-1-pyrroline. The amino acid sequence of the transcription factor OsMPS is as shown in SEQ ID NO. 27. According to the invention, through overexpression of the OsMPS gene driven by the constitutive promoter in the rice, the content of 2-acetyl-1-pyrroline in the rice is obviously increased; and the 2-acetyl-1-pyrroline is not detected in the mutant of the knockout line. The method has the advantages that the OsMPS is used as a positive regulation factor to promote the synthesis of the rice 2-acetyl-1-pyrroline, and plays an important role in improving the rice fragrance quality.

The invention relates to the technical field of rice and ratooning rice aroma enhancement, and discloses a ratooning rice organic aroma enhancer and an aroma enhancement method, the ratooning rice organic aroma enhancer comprises the following raw materials by mass: 6-8 parts of EDTA-Zn, 3-5 parts of monosodium glutamate residue, 1-3 parts of a hair extract, 85 parts of potassium nitrate, 0.5-1.5 parts of ammonium dihydrogen phosphate and 0.5-1.5 parts of diethyl aminoethyl hexanoate. According to the scheme, the ratooning rice organic flavor enhancer adopts various organic substances such as the aginomoto residues and the hair extract, the content of 2-acetyl-1-pyrroline (2-AP) of the aroma of the ratooning season brown rice can be remarkably increased, the quality of the ratooning season rice can be effectively improved, meanwhile, green organic cultivation practice is achieved, and the nutrition and economic value of the ratooning season rice are improved.

The invention discloses application of bacillus albordowii in cultivation for improving the flavor of fragrant rice, and relates to the field of fragrant rice cultivation and planting. According to the application, it is found for the first time that bacillus albordowii has the effect of improving flavor substances of fragrant rice, and when fragrant rice seedlings grow to the three-leaf and one-core stage, the roots of the seedlings are soaked with bacillus albordowii liquid; after the seedlings are transplanted to a field, the bacillus albopastoris bacterial liquid is sprayed to the soil at the roots of the seedlings, 24 main aroma components in the fragrant rice can be increased by 5.56%-12036.00%, the content of 2-ethyl-1-hexanol with rose fragrance is increased by 120 times or above, the content of 2-butyl-1-octanol with flower fragrance is increased by 235.71%, the content of 6, 10-dimethyl-(E)-5, 6-dimethyl-2, 6, 6-tetramethyl-1, 3-pentanedione with fruit fragrance is increased by 235.71%, and the content of 6, 10-dimethyl-(E)-5, 6-tetramethyl-1, 3-pentanedione with fruit fragrance is increased by 235.71%. The content of 2, 9-undecylene-2-ketone is increased by 132.50%, the content of limonene, the content of hexanal, the content of 2-heptanone, the content of 2-tridecanone and the content of 2-pentadecanone are increased by 50% or above, and the content of 2-acetyl-1-pyrroline (2-AP) is increased by 30% or above; the application method is more convenient to operate, and the overall flavor of the rice is improved more remarkably.

The invention relates to the technical field of synthesis of organic compounds, and discloses a preparation method of N-Boc-cis-4-hydroxy-L-proline methyl ester, which comprises the following steps: step 1, synthesis of (2S, 4R)-4-p-toluenesulfonyloxy-1, 2-pyrroline dicarboxylic acid-1-tert-butyl ester-2-methyl ester (2): (2S, 4R)-4-hydroxy-1, 2, 4-triazole-2-methyl ester (3) is prepared from (2S, 4R)-4-hydroxy-1, 2, 4-triazole-2-methyl ester and (2S, 4R)-4-p-toluenesulfonyloxy-1, 2-pyrroline dicarboxylic acid-1- The preparation method comprises the following steps: dissolving 1-tert-butyl-2-methyl 2-pyrroline dicarboxylic acid-1-tert-butyl ester-2-methyl ester in dichloromethane; according to the method, (2S, 4R)-4-hydroxy-1, 2-pyrroline dicarboxylic acid-1-tert-butyl-2-methyl ester which is cheap and easy to obtain in the market is adopted as a raw material, after p-benzoylation and acetylation are performed, hydroxyl of the raw material is converted from trans form to cis form, chiral conversion is performed, and finally the target product is synthesized. The used acetate replaces benzoate, the raw materials are cheaper, and the generated by-product acetic acid is more environment-friendly than benzoic acid, so that the method is suitable for large-scale production.

The invention provides a pyrroline diketone compound as well as a preparation method and a bactericide application thereof. The name of the pyrroline diketone compound is 1-(2, 6-diethyl-4-methylphenyl)-1H-pyrrole-2, 5-diketone, and the structural formula of the pyrroline diketone compound is shown as a formula I. The pyrroline diketone compound and an agricultural bactericide taking the compound as an effective component are used for preventing and treating various plant diseases caused by plant pathogenic fungi, oomycetes and bacteria. The compound is novel in structure and action mechanism, convenient in synthesis method and good in safety, has broad-spectrum and efficient antibacterial activity, does not have cross resistance with other existing bactericides, can be used for preventing and treating plant diseases of vegetables, fruits, food crops, oil crops, commercial crops, garden crops, traditional Chinese medicinal materials and the like, and has broad application prospects. And particularly, the composition has good chemical protection effect and systemic treatment effect on drug-resistant crop diseases.

This invention belongs to the field of crop cultivation technology, specifically relating to a rice nano-flavor enhancer and its application in rice cultivation. The rice nano-flavor enhancer of this invention is prepared from raw materials such as sodium octenyl succinate starch, sodium ascorbate, and sodium selenite. The prepared octenyl succinate starch-nano-selenium has advantages such as small nanoparticle size and system stability. This flavor enhancer can not only significantly improve the overall aroma of fragrant rice, but also enhance the aroma of non-fragrant rice. Specifically, it can significantly increase the content of volatile aroma compounds such as 2-acetyl-1-pyrroline (2-AP) in fragrant rice grains, and significantly increase the content of volatile aroma compounds such as vanillin, benzaldehyde, and geranylacetone in non-fragrant rice grains. Unlike traditional rice flavor enhancers, the rice nano-flavor enhancer of this invention has better applicability and a wider range of applications, helping to meet the growing market demand for high-quality rice.

The formula comprises the following raw materials in parts by weight: 70-85 parts of base liquor, 5-15 parts of fresh pandan leaves, 0.5-3 parts of baked pandan leaf powder, 1-3 parts of a flavor modifier, 3-8 parts of a sugar source and 0.1-0.6 part of a functional additive. The unique flavor of the pandan leaves originates from the rich 2-acetyl-1-pyrroline, and the pandan leaves give out charming rice dumpling fragrance and have the effects of promoting appetite and metabolism at the same time. The pandan leaves are blended into the food, so that the delicious taste is improved, and additional nutritional value is also brought. The pandan leaves are rich in dietary fibers, vitamin B family, vitamin C and various minerals. The composition also contains squalene, linoleic acid, estragole, phytol and other active ingredients. Squalene is a natural organic terpenoid, widely exists in a living body, and has remarkable effects in the aspects of promoting blood circulation, resisting oxidation, resisting inflammation, resisting tumors and the like. The prepared pandan wine has multiple effects and is beneficial to body health after being drunk for a long time.

The application provides a preparation method of a positive electrode additive, comprising the following steps: mixing a fatty amine, an organic carboxylic acid and 5,5-dimethyl-1-pyrroline N-oxide, and performing a hydrothermal reaction to obtain a mixed solution; filtering and freeze-drying the mixed solution to obtain the positive electrode additive; wherein the molar ratio of 5,5-dimethyl-1-pyrroline N-oxide to the fatty amine is <0.8:1, and the molar ratio of 5,5-dimethyl-1-pyrroline N-oxide to the organic carboxylic acid is <0.8:1. The preparation method provided by the application can capture oxygen free radicals in the battery cycle process by introducing 5,5-dimethyl-1-pyrroline N-oxide (DMPO), so as to inhibit the gas production of the battery cell, and can also capture hydroxyl radicals in the battery cycle process, so as to avoid the performance degradation of the battery cell caused by the generation of water. The positive electrode additive can be added to the positive electrode sheet, so that the positive electrode sheet has excellent energy density and cycle performance.

Disclosed are methods of treating malaria with tetrahydrobenzonaphtyridine carboxanilide (TBN) derivatives and related pyrrolinones and hydrolysis products thereof. These compounds are active against Plasmodium falciparum strains that are resistant to multiple drugs currently on the market. The present invention further relates to novel compounds and pharmaceutical compositions comprising such compounds.

The present invention provides a method for interplanting colorful leaves under an agarwood forest, comprising the following steps: S1. forest land selection, S2. colorful leaf seedling selection, S3. forest land arrangement, S4. colorful leaf seedling planting, S5. forest land management, S6. pest control, S7. harvesting. Forest land management is fertilization management and irrigation management. By applying water and fertilizer containing tyrosine, methionine, and methyl jasmonate, the content of 2-acetyl-1-pyrroline in colorful leaves is increased. Pest control effectively prevents and controls powdery mildew, anthracnose, aphids, and mites by spraying a biological insecticide composed of rotenone, osthole, and neem alcohol. The present invention plants colorful leaves under an agarwood forest, and the organic matter and trace elements produced during the growth of colorful leaves provide nutrients for agarwood trees and promote their growth. The covering of colorful leaves reduces the evaporation of water, keeps the soil moist, and is conducive to the growth of agarwood trees. Planting the colorful leaves under the agarwood forest avoids direct sunlight on the colorful leaves, which is conducive to the healthy growth of the colorful leaves.

The invention discloses a method for regulating and controlling enrichment of 2-acetyl-1-pyrroline in germinated brown rice and application of the method. And performing soaking treatment on the brown rice by using an L-proline solution. According to the invention, an L-proline treatment technology and a germination process are combined for the first time, the industrial pain point that the germinated brown rice has nutrition and low aroma is successfully solved, and a high-end product with excellent nutrition and flavor is created.

The invention discloses a 3-ethyl-4-methyl-3-pyrrolin-2-one false template molecularly imprinted adsorption material for removing bilirubin as well as a preparation method and application of the 3-ethyl-4-methyl-3-pyrrolin-2-one false template molecularly imprinted adsorption material. 3-ethyl-4-methyl-3-pyrroline-2-ketone with a molecular structure similar to that of bilirubin is used as a false template molecule, 3-aminopropyltriethoxysilane is used as a functional monomer, tetraethoxysilane is used as a cross-linking agent, absolute ethyl alcohol is used as a solvent, and ultrapure water is used for catalytic hydrolysis to obtain the bilirubin fluorescent probe. A molecularly imprinted polymer adsorbent material is prepared and synthesized by using a sol-gel method; after the false template molecules in the adsorbent material are eluted, imprinted holes left in the material can specifically adsorb bilirubin molecules similar to the false template molecules in structure, so that the purpose of highly selectively adsorbing and removing the bilirubin molecules is achieved. The preparation method disclosed by the invention is simple and convenient to operate, low in cost and high in repeatability, and the 3-ethyl-4-methyl-3-pyrroline-2-one false template molecularly imprinted adsorption material has relatively high adsorption capacity, strong specificity and high biological safety.

This invention discloses a method for improving the aroma of regenerated fragrant rice. Belonging to the field of crop cultivation technology, this method significantly increases the content of the aroma compound 2-acetyl-1-pyrroline (2AP) in regenerated fragrant rice by simultaneously applying paclobutrazol or uniconazole (two plant growth regulators) during the tillering fertilizer application for the first-season fragrant rice, applying urea as a bud-promoting fertilizer before harvesting the first-season fragrant rice, and controlling the harvesting timing and stubble height of the first-season fragrant rice. Simultaneously, it can stabilize or increase the yield of fragrant rice, achieving stable or increased yield while improving the aroma. This invention is beneficial for improving the rice quality of regenerated fragrant rice and promoting its production.

The present invention discloses an imine reductase mutant, a recombinant genetic engineering bacterium and their application in the synthesis of (R)-2-methylpyrrolidine. The imine reductase mutant uses the wild-type imine reductase IR23 with the amino acid sequence shown in SEQ ID NO.1 as a template, and the mutant V249T obtained by mutating valine at position 249, and the mutant V249T / W224F obtained by mutating valine at position 249 and tryptophan at position 224. Through the molecular modification method of semi-rational design, the present invention significantly improves the ability of imine reductase to catalyze the formation of the corresponding cyclic amine from the cyclic imine 2-methylpyrroline. When the mutant V249T / W224F catalyzes 2-methylpyrroline, the substrate conversion rate is 93.3%, which is increased by 49.8% compared with the wild type. The present invention effectively improves the ability of imine reductase to asymmetrically synthesize chiral amines, which is of great significance for the green catalytic synthesis of chiral amine compounds.

The invention discloses a growth-promoting bacterial fertilizer for pandan leaves and application of the growth-promoting bacterial fertilizer, and relates to the technical field of biology. Active components of the growth-promoting bacterial fertilizer for the pandan leaves comprise a microorganism combination, the microbial composition comprises bacillus altitudinis, bacillus megatherium, trichoderma longibrachiatum and aspergillus parasuis. Through the synergistic effect of the four strains, the growth-promoting bacterial fertilizer can effectively improve the soil environment of the saline-alkali soil, reduce the pH value of the soil and improve the enzyme activity of the soil and the nutrient effectiveness of the soil; meanwhile, growth and development of pandan leaves can be remarkably promoted, and plant height, stem diameter, fresh weight of single plant and acre yield are increased; the quality of pandan leaves can be improved, and the content of a characteristic aroma substance 2-acetyl-1-pyrroline is increased. The growth-promoting bacterial fertilizer is simple in preparation process, relatively low in cost, environment-friendly and particularly suitable for popularization and application in planting of pandan leaves in saline-alkali soil, and has important agricultural application value and market prospect.

The application provides a pyrroline dione compound and a preparation method and fungicide application thereof. The pyrroline dione compound is named 1-(2,6-diethyl-4-methylphenyl)-1H-pyrrole-2,5-dione, and a structural formula is shown as formula I: the pyrroline dione compound and an agricultural fungicide with the compound as an effective component are used for preventing and treating various plant diseases caused by plant pathogenic fungi, oomycetes and bacteria. The compound is novel in structure and action mechanism, convenient in synthesis method, good in safety, has broad-spectrum and high-efficiency antibacterial activity, has no cross resistance with other existing fungicides, can be used for preventing and treating plant diseases of vegetables, fruits, food crops, oil crops, economic crops, garden crops and Chinese medicinal materials, and has good chemical protection and internal absorption treatment effects on resistant crop diseases.

To provide a pseudo-dog smell composition which generates a smell similar to a dog smell generated when a dog is kept indoors, for example.SOLUTION: A pseudo-dog smell composition according to an embodiment of the present invention includes: one or more aliphatic saturated hydrocarbons having a carbon number in a range of 8 or more and 20 or less; one or more aliphatic aldehydes having a carbon number in a range of 6 or more and 18 or less; one or more aliphatic monocarboxylic acids having a carbon number in a range of 3 or more and 9 or less; and one or more heterocyclic amines selected from pyrrolines, pyridines, and pyrazines which are optionally substituted with a lower alkyl group or acetyl group.SELECTED DRAWING: None

To provide an extract for imparting the desired 2-acetyl-1-pyrroline aroma to food, a composition containing such an extract, and a method for producing the extract.SOLUTION: The present invention relates to an extract from lettuce (Lactuca sativa), in particular of stalk lettuce (Lactuca sativa var. angustana), comprising 2-acetyl-1-pyrroline and, optionally, precursors of 2-acetyl-1-pyrroline which has been heat-treated, as well as flavoring compositions comprising such an extract and a method for the preparation of such an extract. The invention further concerns the use of such an extract as a taste or flavor ingredient in a consumer product and a method of enhancing, improving, modifying the taste or flavor of a consumer product by making use of such an extract.SELECTED DRAWING: None

The invention provides tobacco essence with milk-flavor rice-flavor style characteristics. The tobacco essence comprises pandanus tectorius absolute oil, and a preparation method of the pandanus tectorius absolute oil comprises the following steps: a, carrying out freeze drying on pre-treated pandanus tectorius, and crushing the pandanus tectorius to obtain pandanus tectorius powder; b, carrying out supercritical carbon dioxide extraction on the pandanus asiatica powder obtained in the step a to obtain a pandanus asiatica extract; and c, carrying out molecular distillation refining on the pandanus tectorius extract to obtain a light component, namely the pandanus tectorius absolute oil. Compared with a water extract or an alcohol extract, the content of a characteristic component, namely 2-acetyl-1-pyrroline, of a rice aroma component in the vanilla absolute oil is higher, the perfume has unique milk aroma, a remarkable rice aroma style can be given to cigarettes, smoke can be softened, irritation can be reduced, offensive odor can be removed, and the smoke amount can be increased.

The application provides a catalytic asymmetric synthesis method of chiral 3-alkyl substituted pyrrolidine, and belongs to the technical field of asymmetric organic synthesis, and comprises the following steps: acyl-protected 3-pyrroline is reacted with alkyl iodide under the catalysis of cobalt and a chiral oxazoline ligand, under the action of a base and a silicon hydride reagent, in an organic solvent, and at 0 DEG C to obtain a chiral 3-alkyl substituted pyrrolidine product. The chiral 3-alkyl substituted pyrrolidine product is obtained in one step, the operation is simple, the regioselectivity is high, the method is cheap and has good application prospect, and can be applied to the efficient synthesis of natural products, drugs and materials.

The application discloses a method for improving drought resistance and oxidation resistance of poplar by improving physiological indexes of the poplar, and the method is irrigating poplar plants with L-glutamic acid solution to improve the physiological indexes of the poplar, including: increasing the relative water content of leaves; reducing the leaf water loss rate; maintaining the stability of the leaf electrolyte permeability; increasing the endogenous proline content of leaves; reducing the expression amount of endogenous proline dehydrogenase, increasing the expression amount of endogenous pyrroline-5-carboxylic acid reductase, reducing the expression amount of endogenous pyrroline-5-carboxylic acid dehydrogenase, and increasing the expression amount of endogenous pyrroline-5-carboxylic acid synthetase; improving the rehydration capacity of drought stress leaves; increasing the content or activity of endogenous catalase; increasing the content or activity of endogenous peroxidase; and increasing the content or activity of endogenous superoxide dismutase.

The application discloses a tridentate phosphine pyrroline ligand, a preparation method thereof, an ethylene oligomerization catalyst and application of the catalyst. The catalyst comprises the tridentate phosphine pyrroline ligand shown in a structure of formula I, a transition metal compound and an alkyl aluminum promoter. The catalyst system can be used for homogeneously catalyzing ethylene oligomerization, and has the advantages of a short catalyst synthesis route, high catalytic activity, and selective generation of 1-octene or 1-hexene by adjusting the amount of the alkyl aluminum promoter.

The invention belongs to the technical field of detection, relates to a free radical detection method, particularly provides a method for detecting water free radical cations by using DMPO, and aims to realize efficient, rapid, simple and convenient water free radical cation detection convenient for analysis. The method comprises the following steps: by taking an electron paramagnetic resonance spectrum technology as a detection tool and 5, 5-dimethyl-1-pyrroline-N-oxide (DMPO) as a spin trapping agent, applying voltage to water, adding a DMPO aqueous solution with a certain concentration into a reacted solution, and carrying out water free radical cation detection on the solution; a corresponding spectrogram is obtained through electron paramagnetic resonance testing, the spectrogram is fitted, and spectrogram characteristic peaks are analyzed to represent water free radical cations. The method can be used for efficiently, quickly and simply detecting the water free radical cations, is convenient for analyzing the water free radical cations, and has popularization value and application prospect.

The invention discloses an ethanol-free pandan scented tablet with an anti-depression effect. The ethanol-free pandan scented tablet is prepared from the following raw materials in parts by weight: 30-50 parts of pandan, 10-20 parts of rose, 5-15 parts of ambrota and 5-10 parts of amber. Through the synergistic effect of the pandan and other natural perfumes, the anti-depression function of the perfumed tablet is achieved. Researches show that the pandan leaves are rich in active ingredients such as 2-acetyl-1-pyrroline (2-AP), beta-caryophyllene and the like, and can stimulate a brain marginal system through an olfactory pathway, promote release of 5-hydroxytryptamine (5-HT) and dopamine (DA) and remarkably improve the emotional state. In addition, linalool of rose and ambrota ether of ambrota in the formula form a synergistic effect, a prefrontal cortex 5-HT1A receptor is activated, the alpha brain wave power spectrum density is enhanced, and rapid emotion regulation is achieved. According to the ethanol-free pandan scented tablet formula, side effects of traditional antidepressant drugs are avoided, a safe and convenient emotion intervention scheme is provided through natural aromatherapy, and the ethanol-free pandan scented tablet formula conforms to the non-drug mental health management trend advocated by WHO.

The present invention provides an olefin polymerization auxiliary agent, an olefin catalyst system, a prepolymerization catalyst composition and an olefin polymerization method, a 3-pyrroline-1-formate compound as an auxiliary agent is added into a Ziegler-Natta type polyolefin catalyst system of which an internal electron donor comprises a diether or succinate compound, and the polymerization activity is maintained at a high level. The activity attenuation is slowed down, and the isotactic index, the molecular weight and the adjustable range of molecular weight distribution of a polymerization product are widened; when applied to random copolymerization, the copolymer has improved polymerization activity, increased ethylene content of the obtained product and reduced melting point of the polymerization product. # imgabs0 #

The present invention relates to a key intermediate for synthesis of a KRAS inhibitor, and a preparation method therefor. Specifically, the present invention provides intermediate compounds as shown in formula I and formula II, and a preparation method therefor, and a method for using the intermediate compound to prepare 2-((S)-4-((S)-7-fluoro-2'-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolizin-7a(5H)-yl)methoxy)-6'-methyl-2,3,5',8'-tetrahydro-6'H-spiro[indene-1,7'-pyrido[4,3-d]pyrimidin]-4'-yl)-1-(2-fluoroacroloyl)piperazin-2-yl)acetonitrile and an analogous compound thereof.

This invention discloses the application of the BnaALDH10A9 protein and its encoding gene in regulating the aroma of Brassica crops. The BnaALDH10A9 protein includes BnaA06.ALDH10A9 protein with the amino acid sequence shown in SEQ ID NO:1 and / or BnaC03.ALDH10A9 protein with the amino acid sequence shown in SEQ ID NO:3. This invention confirms that knocking out the BnaALDH10A9 gene in rapeseed can obtain new Brassica napus germplasm that produces the aroma compound 2AP in different tissues. This invention designs target sites in the exon regions of two copies of the rapeseed BnaALDH10A9 gene (BnaA06.ALDH10A9 and BnaC03.ALDH10A9) and constructs a CRISPR / Cas9 gene editing vector, obtaining knockout mutants through genetic transformation. The aroma compound 2-acetyl-1-pyrrolline (2AP) was detected in the leaves, flowers, and seeds of the mutants (including single and double mutants), and the flowers and rapeseed oil emitted a distinct sweet aroma. This method is suitable for creating new aromatic rapeseed germplasm.