41 results about "Pyrroline" patented technology

This invention belongs to the field of crop cultivation technology, specifically relating to a rice nano-flavor enhancer and its application in rice cultivation. The rice nano-flavor enhancer of this invention is prepared from raw materials such as sodium octenyl succinate starch, sodium ascorbate, and sodium selenite. The prepared octenyl succinate starch-nano-selenium has advantages such as small nanoparticle size and system stability. This flavor enhancer can not only significantly improve the overall aroma of fragrant rice, but also enhance the aroma of non-fragrant rice. Specifically, it can significantly increase the content of volatile aroma compounds such as 2-acetyl-1-pyrroline (2-AP) in fragrant rice grains, and significantly increase the content of volatile aroma compounds such as vanillin, benzaldehyde, and geranylacetone in non-fragrant rice grains. Unlike traditional rice flavor enhancers, the rice nano-flavor enhancer of this invention has better applicability and a wider range of applications, helping to meet the growing market demand for high-quality rice.

The formula comprises the following raw materials in parts by weight: 70-85 parts of base liquor, 5-15 parts of fresh pandan leaves, 0.5-3 parts of baked pandan leaf powder, 1-3 parts of a flavor modifier, 3-8 parts of a sugar source and 0.1-0.6 part of a functional additive. The unique flavor of the pandan leaves originates from the rich 2-acetyl-1-pyrroline, and the pandan leaves give out charming rice dumpling fragrance and have the effects of promoting appetite and metabolism at the same time. The pandan leaves are blended into the food, so that the delicious taste is improved, and additional nutritional value is also brought. The pandan leaves are rich in dietary fibers, vitamin B family, vitamin C and various minerals. The composition also contains squalene, linoleic acid, estragole, phytol and other active ingredients. Squalene is a natural organic terpenoid, widely exists in a living body, and has remarkable effects in the aspects of promoting blood circulation, resisting oxidation, resisting inflammation, resisting tumors and the like. The prepared pandan wine has multiple effects and is beneficial to body health after being drunk for a long time.

The application provides a preparation method of a positive electrode additive, comprising the following steps: mixing a fatty amine, an organic carboxylic acid and 5,5-dimethyl-1-pyrroline N-oxide, and performing a hydrothermal reaction to obtain a mixed solution; filtering and freeze-drying the mixed solution to obtain the positive electrode additive; wherein the molar ratio of 5,5-dimethyl-1-pyrroline N-oxide to the fatty amine is <0.8:1, and the molar ratio of 5,5-dimethyl-1-pyrroline N-oxide to the organic carboxylic acid is <0.8:1. The preparation method provided by the application can capture oxygen free radicals in the battery cycle process by introducing 5,5-dimethyl-1-pyrroline N-oxide (DMPO), so as to inhibit the gas production of the battery cell, and can also capture hydroxyl radicals in the battery cycle process, so as to avoid the performance degradation of the battery cell caused by the generation of water. The positive electrode additive can be added to the positive electrode sheet, so that the positive electrode sheet has excellent energy density and cycle performance.

The invention discloses a method for regulating and controlling enrichment of 2-acetyl-1-pyrroline in germinated brown rice and application of the method. And performing soaking treatment on the brown rice by using an L-proline solution. According to the invention, an L-proline treatment technology and a germination process are combined for the first time, the industrial pain point that the germinated brown rice has nutrition and low aroma is successfully solved, and a high-end product with excellent nutrition and flavor is created.

This invention discloses a method for improving the aroma of regenerated fragrant rice. Belonging to the field of crop cultivation technology, this method significantly increases the content of the aroma compound 2-acetyl-1-pyrroline (2AP) in regenerated fragrant rice by simultaneously applying paclobutrazol or uniconazole (two plant growth regulators) during the tillering fertilizer application for the first-season fragrant rice, applying urea as a bud-promoting fertilizer before harvesting the first-season fragrant rice, and controlling the harvesting timing and stubble height of the first-season fragrant rice. Simultaneously, it can stabilize or increase the yield of fragrant rice, achieving stable or increased yield while improving the aroma. This invention is beneficial for improving the rice quality of regenerated fragrant rice and promoting its production.

The invention discloses a growth-promoting bacterial fertilizer for pandan leaves and application of the growth-promoting bacterial fertilizer, and relates to the technical field of biology. Active components of the growth-promoting bacterial fertilizer for the pandan leaves comprise a microorganism combination, the microbial composition comprises bacillus altitudinis, bacillus megatherium, trichoderma longibrachiatum and aspergillus parasuis. Through the synergistic effect of the four strains, the growth-promoting bacterial fertilizer can effectively improve the soil environment of the saline-alkali soil, reduce the pH value of the soil and improve the enzyme activity of the soil and the nutrient effectiveness of the soil; meanwhile, growth and development of pandan leaves can be remarkably promoted, and plant height, stem diameter, fresh weight of single plant and acre yield are increased; the quality of pandan leaves can be improved, and the content of a characteristic aroma substance 2-acetyl-1-pyrroline is increased. The growth-promoting bacterial fertilizer is simple in preparation process, relatively low in cost, environment-friendly and particularly suitable for popularization and application in planting of pandan leaves in saline-alkali soil, and has important agricultural application value and market prospect.

The application provides a pyrroline dione compound and a preparation method and fungicide application thereof. The pyrroline dione compound is named 1-(2,6-diethyl-4-methylphenyl)-1H-pyrrole-2,5-dione, and a structural formula is shown as formula I: the pyrroline dione compound and an agricultural fungicide with the compound as an effective component are used for preventing and treating various plant diseases caused by plant pathogenic fungi, oomycetes and bacteria. The compound is novel in structure and action mechanism, convenient in synthesis method, good in safety, has broad-spectrum and high-efficiency antibacterial activity, has no cross resistance with other existing fungicides, can be used for preventing and treating plant diseases of vegetables, fruits, food crops, oil crops, economic crops, garden crops and Chinese medicinal materials, and has good chemical protection and internal absorption treatment effects on resistant crop diseases.

To provide a pseudo-dog smell composition which generates a smell similar to a dog smell generated when a dog is kept indoors, for example.SOLUTION: A pseudo-dog smell composition according to an embodiment of the present invention includes: one or more aliphatic saturated hydrocarbons having a carbon number in a range of 8 or more and 20 or less; one or more aliphatic aldehydes having a carbon number in a range of 6 or more and 18 or less; one or more aliphatic monocarboxylic acids having a carbon number in a range of 3 or more and 9 or less; and one or more heterocyclic amines selected from pyrrolines, pyridines, and pyrazines which are optionally substituted with a lower alkyl group or acetyl group.SELECTED DRAWING: None

The application provides a catalytic asymmetric synthesis method of chiral 3-alkyl substituted pyrrolidine, and belongs to the technical field of asymmetric organic synthesis, and comprises the following steps: acyl-protected 3-pyrroline is reacted with alkyl iodide under the catalysis of cobalt and a chiral oxazoline ligand, under the action of a base and a silicon hydride reagent, in an organic solvent, and at 0 DEG C to obtain a chiral 3-alkyl substituted pyrrolidine product. The chiral 3-alkyl substituted pyrrolidine product is obtained in one step, the operation is simple, the regioselectivity is high, the method is cheap and has good application prospect, and can be applied to the efficient synthesis of natural products, drugs and materials.

The application discloses a method for improving drought resistance and oxidation resistance of poplar by improving physiological indexes of the poplar, and the method is irrigating poplar plants with L-glutamic acid solution to improve the physiological indexes of the poplar, including: increasing the relative water content of leaves; reducing the leaf water loss rate; maintaining the stability of the leaf electrolyte permeability; increasing the endogenous proline content of leaves; reducing the expression amount of endogenous proline dehydrogenase, increasing the expression amount of endogenous pyrroline-5-carboxylic acid reductase, reducing the expression amount of endogenous pyrroline-5-carboxylic acid dehydrogenase, and increasing the expression amount of endogenous pyrroline-5-carboxylic acid synthetase; improving the rehydration capacity of drought stress leaves; increasing the content or activity of endogenous catalase; increasing the content or activity of endogenous peroxidase; and increasing the content or activity of endogenous superoxide dismutase.

The application discloses a tridentate phosphine pyrroline ligand, a preparation method thereof, an ethylene oligomerization catalyst and application of the catalyst. The catalyst comprises the tridentate phosphine pyrroline ligand shown in a structure of formula I, a transition metal compound and an alkyl aluminum promoter. The catalyst system can be used for homogeneously catalyzing ethylene oligomerization, and has the advantages of a short catalyst synthesis route, high catalytic activity, and selective generation of 1-octene or 1-hexene by adjusting the amount of the alkyl aluminum promoter.

The invention belongs to the technical field of detection, relates to a free radical detection method, particularly provides a method for detecting water free radical cations by using DMPO, and aims to realize efficient, rapid, simple and convenient water free radical cation detection convenient for analysis. The method comprises the following steps: by taking an electron paramagnetic resonance spectrum technology as a detection tool and 5, 5-dimethyl-1-pyrroline-N-oxide (DMPO) as a spin trapping agent, applying voltage to water, adding a DMPO aqueous solution with a certain concentration into a reacted solution, and carrying out water free radical cation detection on the solution; a corresponding spectrogram is obtained through electron paramagnetic resonance testing, the spectrogram is fitted, and spectrogram characteristic peaks are analyzed to represent water free radical cations. The method can be used for efficiently, quickly and simply detecting the water free radical cations, is convenient for analyzing the water free radical cations, and has popularization value and application prospect.

The present invention relates to a key intermediate for synthesis of a KRAS inhibitor, and a preparation method therefor. Specifically, the present invention provides intermediate compounds as shown in formula I and formula II, and a preparation method therefor, and a method for using the intermediate compound to prepare 2-((S)-4-((S)-7-fluoro-2'-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolizin-7a(5H)-yl)methoxy)-6'-methyl-2,3,5',8'-tetrahydro-6'H-spiro[indene-1,7'-pyrido[4,3-d]pyrimidin]-4'-yl)-1-(2-fluoroacroloyl)piperazin-2-yl)acetonitrile and an analogous compound thereof.

This invention discloses the application of the BnaALDH10A9 protein and its encoding gene in regulating the aroma of Brassica crops. The BnaALDH10A9 protein includes BnaA06.ALDH10A9 protein with the amino acid sequence shown in SEQ ID NO:1 and / or BnaC03.ALDH10A9 protein with the amino acid sequence shown in SEQ ID NO:3. This invention confirms that knocking out the BnaALDH10A9 gene in rapeseed can obtain new Brassica napus germplasm that produces the aroma compound 2AP in different tissues. This invention designs target sites in the exon regions of two copies of the rapeseed BnaALDH10A9 gene (BnaA06.ALDH10A9 and BnaC03.ALDH10A9) and constructs a CRISPR / Cas9 gene editing vector, obtaining knockout mutants through genetic transformation. The aroma compound 2-acetyl-1-pyrrolline (2AP) was detected in the leaves, flowers, and seeds of the mutants (including single and double mutants), and the flowers and rapeseed oil emitted a distinct sweet aroma. This method is suitable for creating new aromatic rapeseed germplasm.

The present invention relates to a self-sealing rubber composition and self-sealing adhesive for tires, and a preparation method therefor. By means of excellent airtightness, flowability and self-adhesion capability of butyl rubber, rubber containing a diene structural unit and liquid polyisobutylene, a micro-crosslinking mode using a pyrrolinone-type crosslinking agent is used to enhance dimensional stability at high temperature, while keeping the flowability; and by means of the reinforcing and filling functions of carbon black and an inorganic filler and the tackifying effect of resin and by means of using the "like dissolves like" principle with the liquid polyisobutylene rubber, the self-sealing rubber composition is prepared by mixing. The positions outside airtight layers of tires corresponding to treads are coated with the prepared rubber composition having the self-sealing property, so as to form self-sealing layer structures; said self-sealing layer structures can ensure the rubber performance in environments of -45℃ to 120℃, and prevent the tires from leaking air when foreign matters below 6 mm puncturing the tires are removed; in addition, foam or other sound-absorption materials can be attached to the self-sealing layer structures to manufacture quiet tires or tires having both self-sealing and soundless functions.

The present application provides a method of producing 2-acetylpyrroline, comprising reacting 2-carboxypyrrline with methylglyoxal. The present application also provides a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 1 or a functional variant thereof, wherein the functional variant has delta(1)-pyrroline-2-carboxylate reductase activity. The present application also provides a method of producing 2-carboxypyrrline, comprising contacting the polypeptide with proline.

The invention discloses a compound with a quinazolinone pyrrole dihydropyrrolinone structure as well as a preparation method and medical application of the compound. The invention provides a compound with a novel quinazolinone pyrrole dihydropyrrolinone structure, and provides a synthesis method of the series of compounds. The compound with the quinazolinone pyrrole dihydropyrrolinone structure has excellent double-target inhibition activity of PIM and HDAC (histone deacetylase). The compound with the quinazolinone pyrrole dihydropyrrolinone structure, provided by the invention, has the application prospect of being developed into a PIM / HDAC double-target inhibitor drug and a drug for treating diseases which are treated or relieved by inhibiting PIM / HDAC double targets. The structure of the compound with the quinazolinone pyrrole dihydropyrrolinone structure is as shown in a formula (1),

The present application belongs to the technical field of microbial inoculants, and particularly relates to a strain of Rhizopus TYR1, a microbial inoculant and application of the microbial inoculant in increasing the content of 2-acetyl-1-pyrroline (2-AP) in rice. The Rhizopus TYR1 and the microbial inoculant containing the Rhizopus TYR1 can increase the content of 2-AP, an aromatic substance, in rice grains and leaves, and the content of 2-AP is significantly increased relative to a control without the Rhizopus TYR1, thereby providing technical support for increasing the aroma concentration in rice. Meanwhile, the present application further provides a method for increasing the content of 2-acetyl-1-pyrroline in rice, which is simple in operation, low in cost, easy to manage and convenient for agricultural promotion.

The application provides adamantane-modified fullerenes (C 60 ) pyrrolines with enhanced fluorescent properties. The application discloses adamantane-modified fullerenes pyrrolines with enhanced fluorescent properties, and a preparation method thereof. The preparation method comprises the following steps: uniformly mixing and dispersing fullerenes, paraformaldehyde, 1-adamantane methylamine and o-dichlorobenzene, reacting, evaporating a solvent under reduced pressure, separating by column chromatography, and recrystallizing to obtain adamantane-modified fullerenes pyrrolines. The adamantane-modified fullerenes pyrrolines are dissolved in m-xylene, and a poor solvent, isopropanol, is slowly added dropwise to form a clear liquid-liquid interface. The reaction liquid is shaken until the liquid-liquid interface disappears, and then is subjected to ultrasonic treatment, standing, precipitation, washing, and drying to obtain microcrystals. The synthesis process is simple, and raw materials are cheap and easy to obtain. The crystallization method is simple and effective, and the crystallization is good, so that the crystal morphology and size can be controlled. The obtained adamantane-modified fullerenes pyrrolines and cubic crystals have good solubility in organic solvents, excellent fluorescent properties, and symmetrical structure, which is beneficial to subsequent application.

The invention discloses indolone spiropyrroline compounds as well as a synthesis method and anticancer activity thereof, and belongs to the technical field of organic synthesis. The preparation method comprises the following steps: mixing an enaminone compound 1, a diazoindolone compound 2, a catalyst, an additive and an organic solvent, and heating to react to prepare the indolone spiropyrroline compound 3. The synthetic method of the indolone spiropyrroline compound is completed through one-pot multi-step cascade reaction of the enaminone compound 1 and the diazoindolone compound 2, and the process is simple and efficient; a design experiment verifies that the compound provided by the invention has the activity of inhibiting proliferation of three cancer cells, namely Hela, SW480 and OVCAR3, and prompts that the compound provided by the invention can be used as an active ingredient of a medicine for resisting cancers such as cervical cancer, colon cancer and ovarian cancer.

The invention belongs to the technical field of microbial agents, and particularly relates to a bacillus aryabhattai bacterium TYR2, a microbial agent and application of the bacillus aryabhattai bacterium TYR2 in increasing the content of 2-acetyl-1-pyrroline (2-acetyl-1-pyrroline, called 2-AP for short) in rice. According to the bacillus aryabhattai bacterium TYR2 and the microbial agent containing the bacillus aryabhattai bacterium TYR2, the content of an aroma substance 2-AP in rice grains and leaves can be increased, the content is remarkably increased compared with that of a contrast without applying the bacillus aryabhattai bacterium TYR2, and a technical support is provided for increasing the aroma concentration in rice. The invention further provides a method for increasing the content of 2-acetyl-1-pyrroline in rice. The method is easy and convenient to operate, low in cost and easy to manage.

The application discloses a kind of essence rice identification method, its steps are: taking the intact rice without being pulverized, adding acetonitrile rapid vortex oscillation extraction, take supernatant and filter, carry out HPLC-MS / MS analysis, whether the sample contains vanillin, methyl vanillin, ethyl vanillin, ethyl maltol, 2-acetylpyridine, 2-propionylpyridine, 2-acetyl-1-pyrroline and 5,6,7,8-tetrahydroquinoxaline at least one, if contains, determine sample as essence rice.The application solves the technical problem that endogenous nature flavor component and artificial essence are effectively distinguished in essence rice identification process, can be used for the rapid qualitative identification of essence rice, method is sensitive, high accuracy.

The application discloses an ethanol-free patchouli incense sheet with an anti-depression effect, which is prepared from the following raw materials in parts by weight: patchouli 30-50 parts, rose 10-20 parts, ambrox 5-15 parts and amber 5-10 parts. The anti-depression function of the incense sheet is realized through the synergistic effect of patchouli and other natural fragrances. Research shows that patchouli leaves are rich in 2-acetyl-1-pyrroline (2-AP) and beta-caryophyllene active ingredients, which can stimulate the brain limbic system through the olfactory pathway, promote the release of 5-hydroxytryptamine (5-HT) and dopamine (DA), and significantly improve the emotional state. In addition, the linalool in the rose and the ambrox in the ambrox form a synergistic effect, activate the prefrontal cortex 5-HT1A receptor, enhance the alpha brain wave power spectrum density, and realize rapid emotional regulation. The ethanol-free patchouli incense sheet formula avoids the side effects of traditional antidepressants, provides a safe and convenient emotional intervention scheme through natural aromatherapy, and meets the non-drug mental health management trend advocated by the WHO.

The invention discloses a preparation method of N-Boc-3-pyrroline, and belongs to the technical field of medical intermediates. The method comprises the following steps: by taking 1, 3-butadiene and N, N-dihalogenated tert-butyl carbamate as raw materials, carrying out 1, 4-cis addition reaction with high stereoselectivity under the catalysis of B (C6F5) 3 to generate (4-halogenated butyl-2-ene-1-yl) tert-butyl carbamate which is mainly cis; and then carrying out ring closing on the cis-(4-halogenated butyl-2-ene-1-yl) tert-butyl carbamate under the action of a phase transfer catalyst and alkali, so as to prepare the N-Boc-3-pyrroline. The preparation method is high in yield, low in cost, simple in technological operation and suitable for industrial production.

The invention relates to a preparation method and application of an oxoindole spiro indanone derivative with optical activity. The compound has a structural formula represented by a general formula I. The synthesis method comprises the following steps: in the presence of a Lewis acid catalyst magnesium trifluoromethanesulfonate and a ligand IV, in an acetonitrile solvent, carrying out Michael addition / cyclization cascade reaction on compounds represented by a general formula II and a general formula III to obtain the compound. Through one-step operation, the 3, 2 '-oxoindole spiro indanone derivative with optical activity can be efficiently synthesized with high stereoselectivity, the yield is 70-99%, dr: 13: 1-gt; 19: 1, ee: 52-99%. The 3, 2 '-pyrroline oxoindole spiro indanone derivative can be used for effectively inhibiting the activity of lung cancer NCI-H460 and colon cancer SW-620 cells, so that the 3, 2'-pyrroline oxoindole spiro indanone derivative can be used as a potential lead compound of a new antitumor drug.

The application discloses a method for synthesizing 2,5-disubstituted pyrroline by a three-enzyme co-immobilization system, and adopts a one-pot cascade biocatalysis method to synthesize 2,5-disubstituted pyrroline. The co-immobilized enzyme obtained by co-immobilizing L-amino transferase, L-lactic acid dehydrogenase and glucose dehydrogenase on an amino resin ESQ-3 is used as a catalyst for a synthesis reaction, and a deep eutectic solvent formed by betaine and glucose is added to a synthesis system. The co-immobilized enzyme is prepared by a method that L-amino transferase is first immobilized on the amino resin ESQ-3, and then L-lactic acid dehydrogenase and glucose dehydrogenase composite particles obtained by ammonium sulfate precipitation are continuously immobilized. The synthesis method can obtain a higher stable conversion rate, and a product is easier to separate and purify. Enzymes and coenzymes are efficiently recycled, regenerated and reused. In addition, a reaction medium added with the deep eutectic solvent has a significant positive effect on solubility of a substrate, stability of the enzyme and mass transfer of the system, so that an immobilized enzyme method catalytic synthesis reaction system suitable for industrial application is effectively constructed.

The invention discloses a pandan flavor green chocolate and a preparation method thereof, and belongs to the technical field of food processing. The chocolate comprises a chocolate matrix and a pandan leaf composite extract, and the extract is prepared by performing enzymolysis wall breaking on pandan leaves, performing extraction by adopting a supercritical carbon dioxide fluid containing d-alpha-tocopherol, and performing microcapsule embedding. Wherein the d-alpha-tocopherol is used as a synergistic stabilizer in the extraction process, and the 2-acetyl-1-pyrroline (2AP) and the chlorophyll are protected in situ. By constructing an integrated process of enzymolysis, low-temperature supercritical extraction containing d-alpha-tocopherol and microcapsule embedding, the 2AP retention rate (greater than or equal to 85.3%), the chlorophyll retention rate (greater than or equal to 89.6%) and the squalene extraction rate (greater than or equal to 82.6%) are remarkably improved, the obtained chocolate is uniform and emerald green and pure in flavor, and the shelf life reaches 10-13 months. The method effectively solves the problems that the flavor of the pandan leaves is easy to lose, the color is easy to change and the activity is difficult to release when the pandan leaves are applied to chocolate.

The application discloses a kind of 2-acetyl-1-pyrroline synthesized by in-situ collection rice root system and its use method in the field of plant biotechnology, the device includes hydroponic box, the upper portion of one side of hydroponic box is connected with first horizontal straight pipe, first horizontal straight pipe is communicated with first vertical pipe by first connecting elbow, first vertical pipe is connected with the water outlet end of water pump, the water inlet end of water pump is connected with liquid inlet by second horizontal straight pipe;The lower portion of the other side of hydroponic box is connected with third horizontal straight pipe, third horizontal straight pipe is communicated with second vertical pipe by second connecting elbow, and second vertical pipe is connected into collection bottle;Hydroponic box top is embedded with cultivation plate, and cultivation plate is provided with cultivation hole, and the junction of cultivation plate and the top of hydroponic box is also provided with sealing element for keeping airtight.The collecting device of the application is simple in structure and easy to build, and 2-acetyl-1-pyrroline synthesized by rice root system is successfully collected, solving the problem that 2-acetyl-1-pyrroline released by rice root system is easy to volatilize and difficult to store.