21 results about "Pyrroline" patented technology

The application provides a preparation method of a positive electrode additive, comprising the following steps: mixing a fatty amine, an organic carboxylic acid and 5,5-dimethyl-1-pyrroline N-oxide, and performing a hydrothermal reaction to obtain a mixed solution; filtering and freeze-drying the mixed solution to obtain the positive electrode additive; wherein the molar ratio of 5,5-dimethyl-1-pyrroline N-oxide to the fatty amine is <0.8:1, and the molar ratio of 5,5-dimethyl-1-pyrroline N-oxide to the organic carboxylic acid is <0.8:1. The preparation method provided by the application can capture oxygen free radicals in the battery cycle process by introducing 5,5-dimethyl-1-pyrroline N-oxide (DMPO), so as to inhibit the gas production of the battery cell, and can also capture hydroxyl radicals in the battery cycle process, so as to avoid the performance degradation of the battery cell caused by the generation of water. The positive electrode additive can be added to the positive electrode sheet, so that the positive electrode sheet has excellent energy density and cycle performance.

The invention discloses a method for regulating and controlling enrichment of 2-acetyl-1-pyrroline in germinated brown rice and application of the method. And performing soaking treatment on the brown rice by using an L-proline solution. According to the invention, an L-proline treatment technology and a germination process are combined for the first time, the industrial pain point that the germinated brown rice has nutrition and low aroma is successfully solved, and a high-end product with excellent nutrition and flavor is created.

This invention discloses a method for improving the aroma of regenerated fragrant rice. Belonging to the field of crop cultivation technology, this method significantly increases the content of the aroma compound 2-acetyl-1-pyrroline (2AP) in regenerated fragrant rice by simultaneously applying paclobutrazol or uniconazole (two plant growth regulators) during the tillering fertilizer application for the first-season fragrant rice, applying urea as a bud-promoting fertilizer before harvesting the first-season fragrant rice, and controlling the harvesting timing and stubble height of the first-season fragrant rice. Simultaneously, it can stabilize or increase the yield of fragrant rice, achieving stable or increased yield while improving the aroma. This invention is beneficial for improving the rice quality of regenerated fragrant rice and promoting its production.

The invention discloses a growth-promoting bacterial fertilizer for pandan leaves and application of the growth-promoting bacterial fertilizer, and relates to the technical field of biology. Active components of the growth-promoting bacterial fertilizer for the pandan leaves comprise a microorganism combination, the microbial composition comprises bacillus altitudinis, bacillus megatherium, trichoderma longibrachiatum and aspergillus parasuis. Through the synergistic effect of the four strains, the growth-promoting bacterial fertilizer can effectively improve the soil environment of the saline-alkali soil, reduce the pH value of the soil and improve the enzyme activity of the soil and the nutrient effectiveness of the soil; meanwhile, growth and development of pandan leaves can be remarkably promoted, and plant height, stem diameter, fresh weight of single plant and acre yield are increased; the quality of pandan leaves can be improved, and the content of a characteristic aroma substance 2-acetyl-1-pyrroline is increased. The growth-promoting bacterial fertilizer is simple in preparation process, relatively low in cost, environment-friendly and particularly suitable for popularization and application in planting of pandan leaves in saline-alkali soil, and has important agricultural application value and market prospect.

The application provides a pyrroline dione compound and a preparation method and fungicide application thereof. The pyrroline dione compound is named 1-(2,6-diethyl-4-methylphenyl)-1H-pyrrole-2,5-dione, and a structural formula is shown as formula I: the pyrroline dione compound and an agricultural fungicide with the compound as an effective component are used for preventing and treating various plant diseases caused by plant pathogenic fungi, oomycetes and bacteria. The compound is novel in structure and action mechanism, convenient in synthesis method, good in safety, has broad-spectrum and high-efficiency antibacterial activity, has no cross resistance with other existing fungicides, can be used for preventing and treating plant diseases of vegetables, fruits, food crops, oil crops, economic crops, garden crops and Chinese medicinal materials, and has good chemical protection and internal absorption treatment effects on resistant crop diseases.

The application provides a catalytic asymmetric synthesis method of chiral 3-alkyl substituted pyrrolidine, and belongs to the technical field of asymmetric organic synthesis, and comprises the following steps: acyl-protected 3-pyrroline is reacted with alkyl iodide under the catalysis of cobalt and a chiral oxazoline ligand, under the action of a base and a silicon hydride reagent, in an organic solvent, and at 0 DEG C to obtain a chiral 3-alkyl substituted pyrrolidine product. The chiral 3-alkyl substituted pyrrolidine product is obtained in one step, the operation is simple, the regioselectivity is high, the method is cheap and has good application prospect, and can be applied to the efficient synthesis of natural products, drugs and materials.

The present application provides a method of producing 2-acetylpyrroline, comprising reacting 2-carboxypyrrline with methylglyoxal. The present application also provides a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 1 or a functional variant thereof, wherein the functional variant has delta(1)-pyrroline-2-carboxylate reductase activity. The present application also provides a method of producing 2-carboxypyrrline, comprising contacting the polypeptide with proline.

The present application belongs to the technical field of microbial inoculants, and particularly relates to a strain of Rhizopus TYR1, a microbial inoculant and application of the microbial inoculant in increasing the content of 2-acetyl-1-pyrroline (2-AP) in rice. The Rhizopus TYR1 and the microbial inoculant containing the Rhizopus TYR1 can increase the content of 2-AP, an aromatic substance, in rice grains and leaves, and the content of 2-AP is significantly increased relative to a control without the Rhizopus TYR1, thereby providing technical support for increasing the aroma concentration in rice. Meanwhile, the present application further provides a method for increasing the content of 2-acetyl-1-pyrroline in rice, which is simple in operation, low in cost, easy to manage and convenient for agricultural promotion.

The application provides adamantane-modified fullerenes (C 60 ) pyrrolines with enhanced fluorescent properties. The application discloses adamantane-modified fullerenes pyrrolines with enhanced fluorescent properties, and a preparation method thereof. The preparation method comprises the following steps: uniformly mixing and dispersing fullerenes, paraformaldehyde, 1-adamantane methylamine and o-dichlorobenzene, reacting, evaporating a solvent under reduced pressure, separating by column chromatography, and recrystallizing to obtain adamantane-modified fullerenes pyrrolines. The adamantane-modified fullerenes pyrrolines are dissolved in m-xylene, and a poor solvent, isopropanol, is slowly added dropwise to form a clear liquid-liquid interface. The reaction liquid is shaken until the liquid-liquid interface disappears, and then is subjected to ultrasonic treatment, standing, precipitation, washing, and drying to obtain microcrystals. The synthesis process is simple, and raw materials are cheap and easy to obtain. The crystallization method is simple and effective, and the crystallization is good, so that the crystal morphology and size can be controlled. The obtained adamantane-modified fullerenes pyrrolines and cubic crystals have good solubility in organic solvents, excellent fluorescent properties, and symmetrical structure, which is beneficial to subsequent application.

The invention belongs to the technical field of microbial agents, and particularly relates to a bacillus aryabhattai bacterium TYR2, a microbial agent and application of the bacillus aryabhattai bacterium TYR2 in increasing the content of 2-acetyl-1-pyrroline (2-acetyl-1-pyrroline, called 2-AP for short) in rice. According to the bacillus aryabhattai bacterium TYR2 and the microbial agent containing the bacillus aryabhattai bacterium TYR2, the content of an aroma substance 2-AP in rice grains and leaves can be increased, the content is remarkably increased compared with that of a contrast without applying the bacillus aryabhattai bacterium TYR2, and a technical support is provided for increasing the aroma concentration in rice. The invention further provides a method for increasing the content of 2-acetyl-1-pyrroline in rice. The method is easy and convenient to operate, low in cost and easy to manage.

The invention discloses a preparation method of N-Boc-3-pyrroline, and belongs to the technical field of medical intermediates. The method comprises the following steps: by taking 1, 3-butadiene and N, N-dihalogenated tert-butyl carbamate as raw materials, carrying out 1, 4-cis addition reaction with high stereoselectivity under the catalysis of B (C6F5) 3 to generate (4-halogenated butyl-2-ene-1-yl) tert-butyl carbamate which is mainly cis; and then carrying out ring closing on the cis-(4-halogenated butyl-2-ene-1-yl) tert-butyl carbamate under the action of a phase transfer catalyst and alkali, so as to prepare the N-Boc-3-pyrroline. The preparation method is high in yield, low in cost, simple in technological operation and suitable for industrial production.

The application discloses a method for synthesizing 2,5-disubstituted pyrroline by a three-enzyme co-immobilization system, and adopts a one-pot cascade biocatalysis method to synthesize 2,5-disubstituted pyrroline. The co-immobilized enzyme obtained by co-immobilizing L-amino transferase, L-lactic acid dehydrogenase and glucose dehydrogenase on an amino resin ESQ-3 is used as a catalyst for a synthesis reaction, and a deep eutectic solvent formed by betaine and glucose is added to a synthesis system. The co-immobilized enzyme is prepared by a method that L-amino transferase is first immobilized on the amino resin ESQ-3, and then L-lactic acid dehydrogenase and glucose dehydrogenase composite particles obtained by ammonium sulfate precipitation are continuously immobilized. The synthesis method can obtain a higher stable conversion rate, and a product is easier to separate and purify. Enzymes and coenzymes are efficiently recycled, regenerated and reused. In addition, a reaction medium added with the deep eutectic solvent has a significant positive effect on solubility of a substrate, stability of the enzyme and mass transfer of the system, so that an immobilized enzyme method catalytic synthesis reaction system suitable for industrial application is effectively constructed.

The invention discloses a pandan flavor green chocolate and a preparation method thereof, and belongs to the technical field of food processing. The chocolate comprises a chocolate matrix and a pandan leaf composite extract, and the extract is prepared by performing enzymolysis wall breaking on pandan leaves, performing extraction by adopting a supercritical carbon dioxide fluid containing d-alpha-tocopherol, and performing microcapsule embedding. Wherein the d-alpha-tocopherol is used as a synergistic stabilizer in the extraction process, and the 2-acetyl-1-pyrroline (2AP) and the chlorophyll are protected in situ. By constructing an integrated process of enzymolysis, low-temperature supercritical extraction containing d-alpha-tocopherol and microcapsule embedding, the 2AP retention rate (greater than or equal to 85.3%), the chlorophyll retention rate (greater than or equal to 89.6%) and the squalene extraction rate (greater than or equal to 82.6%) are remarkably improved, the obtained chocolate is uniform and emerald green and pure in flavor, and the shelf life reaches 10-13 months. The method effectively solves the problems that the flavor of the pandan leaves is easy to lose, the color is easy to change and the activity is difficult to release when the pandan leaves are applied to chocolate.

The application discloses a kind of 2-acetyl-1-pyrroline synthesized by in-situ collection rice root system and its use method in the field of plant biotechnology, the device includes hydroponic box, the upper portion of one side of hydroponic box is connected with first horizontal straight pipe, first horizontal straight pipe is communicated with first vertical pipe by first connecting elbow, first vertical pipe is connected with the water outlet end of water pump, the water inlet end of water pump is connected with liquid inlet by second horizontal straight pipe;The lower portion of the other side of hydroponic box is connected with third horizontal straight pipe, third horizontal straight pipe is communicated with second vertical pipe by second connecting elbow, and second vertical pipe is connected into collection bottle;Hydroponic box top is embedded with cultivation plate, and cultivation plate is provided with cultivation hole, and the junction of cultivation plate and the top of hydroponic box is also provided with sealing element for keeping airtight.The collecting device of the application is simple in structure and easy to build, and 2-acetyl-1-pyrroline synthesized by rice root system is successfully collected, solving the problem that 2-acetyl-1-pyrroline released by rice root system is easy to volatilize and difficult to store.

The application discloses an electro-synthesis method of 1-pyrroline, which comprises the following steps: using a platinum sheet as a cathode, a carbon rod as an anode, using tetrabutylammonium perchlorate or tetrabutylammonium tetrafluoroborate as an electrolyte under constant current catalysis, and one-pot dimerization cyclization of styrene and azidotrimethylsilane to prepare 1 ‑ pyrroline derivative, and after post-treatment of the obtained reaction mixture, 1 ‑ pyrroline is obtained through column chromatography separation. ‑ The application can obtain 1 ‑ pyrroline derivative through only one reaction step, has few operation steps, avoids use of oxidants, additives and metals, and has a simple post-treatment process and easy product separation.

Provided in the present application are a pyrrolinedione compound, and a preparation method therefor and the use thereof as a microbicide. The pyrrolinedione compound is 1-(2,6-diethyl-4-methylphenyl)-1H-pyrrole-2,5-dione. The pyrrolinedione compound and an agricultural microbicide using the pyrrolinedione compound as an active ingredient are used for controlling a variety of plant diseases caused by phytopathogenic fungi, oomycetes and bacteria. The pyrrolonedione compound has a novel structure and action mechanism, a convenient synthesis method and good safety, exhibits a broad-spectrum and high-efficiency antimicrobial activity, shows no cross resistance with other existing microbicides, can be used for controlling plant diseases in vegetables, fruits, grain crops, oil crops, economic crops, landscape crops and Chinese medicinal materials, and has a particularly good chemical protective effect and systemic therapeutic effect on diseases in drug-resistant crops.

The application provides a catalytic asymmetric synthesis method of chiral 2-alkyl substituted pyrrolidine, belongs to the technical field of asymmetric organic synthesis, and relates to the reaction of acyl-protected 3-pyrroline and alkyl iodide under the catalysis of nickel and a chiral oxazoline ligand, under the action of a base and a silicon hydride reagent, in an organic solvent and at room temperature to obtain a chiral 2-alkyl substituted pyrrolidine product. The application is simple in operation, high in regioselectivity, is a cheap method with good application prospect, and can be applied to the efficient synthesis of natural products, drugs and materials.

The invention belongs to the field of molecular biology and biotechnology, and relates to a wheat fragrance quality regulation gene TaBADH2 as well as a molecular marker and application thereof. According to the invention, the TaBADH2 gene for regulating and controlling the wheat fragrance is cloned from the genome of Zhengmai 1860. The cds of the TaBADH2 gene on a genome A, a genome B and a genome D are respectively TaBADH2-A cds, TaBADH2-B cds and TaBADH2-D cds, and the cds are shown as SEQ ID NO: 1 to SEQ ID NO: 3. A conserved cds region of TaBADH2 is further utilized to construct a VIGS vector, wheat is transformed, and the result shows that the content of 2-acetyl-1-pyrroline (2AP) in gene silent wheat is obviously improved compared with that in control wheat. Therefore, the fragrance quality of the gene silencing type wheat can be improved. Meanwhile, a Del type molecular marker is developed according to clone sequence information, and fragrance differences among different wheat varieties can be rapidly judged by utilizing the marker.

[Problem] The purpose of the inventors of the present invention is to provide instant noodles having good texture, saltiness and frying aroma even when the content of sodium is reduced. [Solution] The present inventors conducted intensive research on a substance for mitigating the reduction of flavor that occurs when the sodium content of soup base used for instant noodles is reduced. The present inventors have found that it is possible to provide instant fried noodles having an excellent salty taste and a good frying aroma while suppressing the amount of sodium used by containing 2-acetyl-1-pyrroline, and have completed the invention. In addition, it is recognized that the flavor improving effect is enhanced by adding an aliphatic aldehyde or isothiocyanate to 2-acetyl-1-pyrroline.