811 results about "Bacillus velezensis" patented technology

The invention relates to bacillus velezensis SMB1 and application thereof in prevention and treatment of plant diseases, and belongs to the technical field of biological prevention and treatment of plant diseases. The strain is preserved in the China Center for Type Culture Collection on November 27, 2024, the preservation address is Luojia mountain, Luojia mountain, eight road, Wuchang District, Wuhan City, Hubei Province, and the preservation number is CCTCC NO: M20242647. The bacillus velezensis SMB1 provided by the invention has a remarkable bacteriostatic effect on plant pathogenic fungi, plant pathogenic oomycetes, plant pathogenic bacteria and animal pathogenic bacteria, has an extremely good broad-spectrum bacteriostatic function, has a great application potential in biological control of plant diseases, can reduce the use of chemical pesticides, and has a good application prospect. The pesticide residue in agricultural products is reduced, the environment is protected, and the urgent demand on pollution-free agricultural product production at present is met.

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention discloses a growth-promoting bacillus velezensis FSUXF-717 capable of tolerating heavy metals and application of the growth-promoting bacillus velezensis FSUXF-717, and belongs to the technical field of agricultural microorganisms. The bacillus velezensis FSUXF-717 has the following advantages: (1) the bacillus velezensis FSUXF-717 has multifunctional metabolic characteristics: various carbon sources such as cane sugar, raffinose and aesculin can be utilized, and the bacillus velezensis FSUXF-717 has the functions of producing long-chain hormone, dissolving phosphorus, secreting ACC deaminase, efficiently forming a biological membrane and the like; (2) broad-spectrum adaptability: the strain has strong tolerance to heavy metal pollution (Cd / Pb / Cr / Ni), drought and broad-spectrum acid-base stress (pH 4.5-9.5); under the condition of relatively low bacterium concentration, the auxin can be stably synthesized in a heavy metal-acid-base composite stress environment. And (3) the activity of soil sucrase and soil phosphatase is enhanced. Excavation of the strain can provide an important germplasm resource basis for development and utilization of novel efficient microbial fertilizers and lay a foundation for green and high-quality development of agriculture.

The invention belongs to the technical field of microorganisms, particularly relates to rice endophytic bacillus velezensis AN6 and application thereof, and particularly provides bacillus velezensis AN6 which is classified and named as bacillus velezensis and has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20231886. The invention provides a novel rice endophytic bacillus velezensis strain AN6, through separation, screening, identification and experimental verification, it is found that the strain shows broad-spectrum resistance to various phytopathogens including rice bacterial leaf blight, rice blast and the like, the strain has the capacity of inhibiting various phytopathogens and has the capacity of adsorbing heavy metal, and the strain has the advantages of being capable of inhibiting various phytopathogens and capable of being used as a novel rice endophytic bacillus velezensis strain AN6. The method is of great significance to biological control of rice diseases and development of green agriculture.

The invention relates to bacillus velezensis and application thereof in preparation of a multifunctional straw decomposition agent, and belongs to the technical field of microorganisms. The bacillus velezensis has the functions of producing cellulase, producing xylanase, degrading abamectin, decomposing inorganic phosphorus and degrading straws; when straw is returned to the field, the multifunctional straw decomposition agent provided by the invention not only can accelerate the decomposition speed of straw, decompose abamectin residues in soil and improve the soil ecology, but also can promote the growth of pepper plants and enhance the stress resistance of the pepper plants, and has a good comprehensive application effect.

The invention discloses bacillus velezensis capable of tolerating various environmental pollution and promoting growth and application of the bacillus velezensis. A plant endophyte bacillus velezensis L381 strain is identified in wild rice, and the strain is high in environmental adaptability, can grow in the environment with the temperature of 15-40 DEG C and the pH value of 5-8, and can tolerate the NaCl concentration of 4%; the rice growth promoting agent has a good growth promoting function, can secrete auxin IAA, solubilize phosphorus, dissolve potassium and secrete siderophores, and can effectively promote rice growth. Meanwhile, the strain has stress resistance to environmental stress such as heavy metals and antibiotics, and tetracycline can be degraded; the strain has a wide antibacterial spectrum, and can be used as a biocontrol bacterium for preventing and treating various plant diseases. The plant endophyte L381 can adapt to variable temperature conditions and variable soil environments in the natural environment, has very strong practical application potential, not only improves the functions of bacillus velezensis, but also widens the application range of the strain.

The invention discloses a saline-alkaline tolerant growth-promoting bacillus velezensis AH216 and application thereof, and belongs to the technical field of microbiology and biologica.Bacillus velezensis AH216 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC No: M 20241496. The saline-alkaline tolerant growth-promoting bacillus velezensis AH216 has the advantages that the saline-alkaline tolerant growth-promoting bacillus velezensis AH216 can be used for preparing saline-alkaline tolerant growth-promoting bacillus velezensis AH216; the bacillus velezensis AH216 provided by the invention can inhibit the growth of pathogenic bacteria of cotton fusarium wilt, and has the growth promoting functions of dissolving potassium, generating indoleacetic acid, cellulase, protease, siderophore and the like; meanwhile, the bacillus velezensis AH216 can tolerate a highly saline-alkaline environment, can improve agronomic characters such as the plant height, the biomass and the leaf area in the corn seedling stage in common soil and saline-alkaline soil, and can promote the development of a corn root system.

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention belongs to the technical field of plant disease control, and particularly relates to bacillus velezensis and application thereof. The bacillus velezensis strain is separated and screened from rhizosphere soil of wheat in a main wheat area in Hebei Province, the growth characteristics and physiological and biochemical characteristics of the bacterial colony are identified, then 16S rDNA sequencing is carried out, Blast homology comparison is carried out in a GenBank database, the bacterial strain is identified to be bacillus velezensis through the analysis, and the bacillus velezensis strain is identified to be bacillus velezensis. The fungicide containing the bacillus velezensis has a good prevention and treatment effect on wheat stem rot through seed coating.

The invention relates to bacillus velezensis, a biocontrol inoculant prepared from the bacillus velezensis and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus velezensis provided by the invention is bacillus velezensis and is named as JK1, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31375, and the preservation time is July 19, 2024. The bacillus JK1 disclosed by the invention has relatively excellent bacteriostatic action on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus JK1 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention discloses bacillus velezensis and application of the bacillus velezensis in prevention and treatment of basal stem rot, the classification name of the bacillus velezensis is bacillus velezensis GN-22, the bacillus velezensis is preserved in China Center for Type Culture Collection (CCTCC), the preservation number of the bacillus velezensis is CCTCC NO: M2025566, and the preservation date is March 24, 2025. The bacillus velezensis GN-22 disclosed by the invention belongs to rhizosphere growth-promoting bacteria, has a remarkable inhibition effect on different pathogenic fungi such as fusarium graminearum, fusarium pseudograminearum, fusarium asiaticum, fusarium verticillioides and fusarium oxysporum, and plays an important role in biological control of diseases such as corn and wheat stem rot; meanwhile, the strain has a growth promoting effect and can promote corn growth. The strain provided by the invention has the advantages of simple culture conditions, easiness in preservation, easiness in industrial production and good development and application prospects.

The invention discloses bacillus velezensis Scm3 and application thereof.The bacillus velezensis Scm3 is obtained by screening a strain Scm with an inhibition effect on aeromonas hydrophila from turbot intestinal contents and then conducting ultraviolet mutagenesis on the strain to obtain a strain Scm3 with an enhanced antagonism effect on aeromonas hydrophila, and the strain Scm3 is applied to the preparation of the bacillus velezensis Scm3 and the application of the bacillus velezensis Scm3 to prepare the bacillus velezensis Scm3. 16SrDNA (deoxyribonucleic acid) sequence identification and genome sequencing prove that the strain is bacillus velezensis, and the inhibition mechanism of the bacillus velezensis Scm3 on aeromonas hydrophila is researched by a non-contact co-culture method.

The invention belongs to the technical field of agriculture, and particularly provides a compound microbial agent and application thereof. The compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02. The compound microbial agent can effectively improve the pH and EC of saline alkali soil, increase the content of alkali-hydrolyzable nitrogen, available phosphorus and quick-acting potassium in the soil, and play a significant role in increasing the plant height, stem diameter, chlorophyll content and aboveground biomass of corn plants in coastal saline-alkali soil.

The invention relates to the technical field of microorganisms and bio-organic fertilizers, and particularly discloses a bio-organic fertilizer for preventing and treating rhizome diseases of horseradish and application of the bio-organic fertilizer, the bio-organic fertilizer comprises the following components: component A: a bacillus velezensis microbial inoculum with the preservation number of CCTCC NO: M 2022923 and a trichoderma yunnanense microbial inoculum with the preservation number of CCTCC NO: M 2025645; the component B is prepared from soybean meal, vinegar residues, charcoal, wollastonite powder and allicin. The biological organic fertilizer disclosed by the invention can be used for increasing the content of growth hormones such as salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) and phytoalexin as well as the activity of chitinase and beta-1, 3-glucanase of a wasabi root system, and has a relatively good prevention and treatment effect on a black heart disease caused by infection of phoma wabi Yokog and a soft rot disease caused by infection of pectobacterium carotovorum subsp. Carotovorum (Pcc). The biological organic fertilizer disclosed by the invention has a relatively good prevention and treatment effect on the soft rot disease caused by infection of the phoma wabi Yokog and a relatively good prevention and treatment effect on the soft rot disease caused by infection of the phoma wabi subsp. Carotovorum (Pcc).

Optimization of the formula of a culture medium for industrially producing a Bacillus velezensis TCS001 fermentation broth and culture process conditions therefor. By using the culture medium and the culture process conditions, not only can the fermentation production cost be reduced, but also the effective viable bacterium count and the spore count in the Bacillus velezensis TCS001 fermentation broth can be improved, so that the fermentation broth can be used for industrial production and market promotion and application. Provided is a lipopeptide-compound-containing mixture separated from a Bacillus velezensis TCS001 fermentation broth. By further optimizing the formula of the culture medium for the Bacillus velezensis TCS001 fermentation broth and the culture fermentation process conditions, the output and yield of lipopeptide compounds in the fermentation broth are successfully improved, providing an efficient and cost-effective solution for the control of plant diseases.

The invention relates to the technical field of microorganisms and particularly discloses bacillus velezensis for producing tetramethylpyrazine and application of the bacillus velezensis, the preservation number of the strain is CCTCC NO: M20232058, and the bacillus velezensis is obtained by separating and purifying from high-temperature Daqu of Maotai-flavor liquor in the liquor industry of Jin-brand Maotai Town in Guizhou. The bacillus velezensis disclosed by the invention can produce 1875.4 mg / L of tetramethylpyrazine by performing shake flask fermentation on a liquid culture medium for 7 days, and the tetramethylpyrazine can reach 4810.8 mg / L by optimizing a culture medium formula and culture conditions. The bacillus velezensis is applied to small-scale brewing of Maotai-flavor liquor, the content of tetramethylpyrazine in the Maotai-flavor liquor reaches 34.5 + / -1.4 mg / L and is increased by 325.93% compared with a contrast, and the bacillus velezensis can be used for increasing the content of tetramethylpyrazine in the Maotai-flavor liquor.

The invention discloses bacillus velezensis XF-8 and application of the bacillus velezensis XF-8 in prevention and treatment of tomato bacterial wilt caused by Ralstonia solanacearum, and belongs to the technical field of microorganisms. The bacillus velezensis XF-8 capable of antagonizing Ralstonia solanacearum is obtained through separation and screening, and it is found that the bacillus velezensis XF-8 also has an excellent inhibition effect on various pathogenic fungi and shows broad-spectrum antibacterial activity of the bacillus velezensis XF-8. The bacillus velezensis XF-8 has a good inhibition effect on tomato bacterial wilt, and the control effect of the bacillus velezensis XF-8 in potted tomatoes reaches 74.25%. Results of the invention show that the bacillus velezensis XF-8 has a great application prospect in prevention and treatment of tomato bacterial wilt, and a good biocontrol resource is provided for research and development of a biocontrol agent for tomato bacterial wilt.

The invention belongs to the technical field of potato scab prevention and treatment, and particularly relates to a complex microbial inoculant for preventing and treating potato scab and application of the complex microbial inoculant. The complex microbial inoculant provided by the invention comprises bacillus velezensis BEV2 and bacillus atrophaeus FM2-4, wherein the effective viable bacterium concentration of the bacillus velezensis BEV2 and the effective viable bacterium concentration of the bacillus atrophaeus FM2-4 in the complex microbial inoculant are respectively 1-3 * 10 < 8 > CFU / m. The complex microbial inoculant provided by the invention can effectively prevent and treat the potato scab, reduce the morbidity of the potato scab and improve the yield and quality of potatoes, and has the advantages of being not easily influenced by the environment and being harmless to the environment.

The invention relates to the technical field of microorganisms, in particular to a disease-resistant, growth-promoting and yield-increasing strain and a microbial agent and application thereof. The strain is a strain bacillus velezensis NB21 (CCTCC (China Center for Type Culture Collection) NO: M2024979) which is found autonomously; the strain can grow at low temperature, is strong in ecological environment adaptability, can be used for preparing a single microbial agent or a compound microbial agent, and can also be used for forming a composition with other functional organic and inorganic substances. The prepared microbial agent can significantly prevent and treat diseases, promote root development of crops such as rice and corn, enhance photosynthesis, improve system resistance, increase crop yield, enhance nutrient supply and improve soil micro-ecology. The strain is unique, strong in colonization ability and diversified in function, the prepared microbial agent fermentation production technology is mature, the cost is low, the application range is wide, the yield increasing and quality improving effects are outstanding, and the microbial agent has good application prospects.

The invention provides a bacillus velezensis strain and application thereof. The name of the bacillus velezensis is BJ-1141, and the bacillus velezensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32094. The strain has broad-spectrum antibacterial activity, and has a good inhibition effect on 24 plant pathogenic fungi such as rice pathogenic bacteria, wheat pathogenic bacteria, corn pathogenic bacteria, grape pathogenic bacteria, cherry pathogenic bacteria, solanaceous crop pathogenic bacteria and the like. Wherein the growth inhibition effect on sclerotinia sclerotiorum hyphae causing eggplant sclerotinia rot is optimal, and the inhibition rate can reach 95.57%. The BJ-1141 bacterial liquid has an obvious growth promoting effect on the growth of plants such as tomatoes and lettuce. After the BJ-1141 bacterial liquid is used for irrigating roots of potted tomatoes, the tomato fusarium wilt caused by tomato fusarium oxysporum can be effectively controlled, the BJ-1141 bacterial liquid has a good prevention and treatment effect, the prevention and treatment effect can reach 85.93%, and the prevention and treatment effect is obviously different from that of a control group.

The invention relates to a microbial agent prepared based on a new strain of bacillus velezensis, which has the function of broad-spectrum antagonism of common pathogenic bacteria of aquatic products and can effectively prevent and / or treat bacterial diseases and / or fungal diseases of aquatic organisms including crustaceans and fishes. Meanwhile, the content of nitrite and ammonia nitrogen in aquaculture water can be effectively reduced, and the usage amount and residual quantity of antibiotics and chemical drugs in aquaculture are reduced.

The invention discloses an organic fertilizer for promoting plant growth and improving soil and a preparation method thereof, and belongs to the technical field of organic fertilizers. The organic fertilizer is prepared from the following raw materials: composted chicken manure, soybean meal, straw powder, sweet potato vines, humic acid, a compound microbial agent, rosmarinic acid and a stimulant. The compound microbial agent is prepared from madula angustifolia actinomycetes, pseudomonas stutzeri and bacillus velezensis, and the stimulant is a glucosamine sulfate solution. The rosmarinic acid, the glucosamine sulfate solution and the compound microbial agent are added into the organic fertilizer, the three components are compounded with the straw, the chicken manure and the like to form the organic fertilizer, and various substances have a synergistic effect, so that on one hand, the growth of Chinese cabbages is promoted, and the yield is increased; on the other hand, soil physicochemical properties are obviously improved.

The invention relates to the technical field of new strains, and provides a new strain which is screened from Xinjiang saline-alkali soil, is classified and named as sphingomonas sp. And is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCCNO.32220. Research finds that the strain can remarkably reduce the soil conductivity, the exchangeable sodium content, the alkalization degree and the total salt content, so that the soil quality is improved. The organic matter content and the acid phosphatase activity of the soil are remarkably improved, and the quality of the saline-alkali soil can be efficiently improved. Meanwhile, the invention provides a compound bacterial fertilizer for effectively improving saline-alkali soil, the compound bacterial fertilizer comprises bacillus subtilis, Exiguobacterium acetylbacter, Bacillus velezensis, Pseudomonas fluorescens, Rhodococcus capsulatus and a new strain Sphingomonas sp., and research finds that compared with a single bacterial solution, the compound bacterial solution has a more prominent remediation effect on saline-alkali soil.

The invention discloses a method for increasing the content of pyrazines and 2, 3-butanediol substances through high-temperature Daqu micro-ecological regulation and control, and belongs to the technical field of microbial food processing. The invention discloses a method for improving the content of pyrazines and 2, 3-butanediol substances through micro-ecological regulation and control of high-temperature yeast for making hard liquor, and the method comprises the following steps of: combining and inoculating strains, namely bacillus subtilis CGMCC (China General Microbiological Culture Collection Center) 10830, bacillus velezensis CGMCC 7044 and bacillus licheniformis CGMCC 20675, into wheat flour according to a proportion to prepare the high-temperature yeast for making hard liquor. According to the invention, not only are the contents of pyrazine substances and 2, 3-butanediol increased, but also the abundance of beneficial microorganisms such as bacillus, thermophilic fungi and the like is remarkably improved, and a good saccharification fermentation agent is provided for brewing high-quality white spirit.

The invention provides bacillus velezensis and application of the bacillus velezensis in co-production of 6-methyl-2-heptanone and 2, 4-di-tert-butylphenol, and belongs to the technical field of biological engineering. The bacillus velezensis is classified and named as bacillus velezensis LT-22, and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M2023779, and the preservation date is May 19, 2023. According to the bacillus velezensis, the 6-methyl-2-heptanone and the 2, 4-di-tert-butylphenol can be co-produced, and the concentrations of the synthesized 6-methyl-2-heptanone and the 2, 4-di-tert-butylphenol can reach 75.72 mg / L and 42.48 mg / L; compared with chemical synthesis, the method for preparing the 6-methyl-2-heptanone and the 2, 4-di-tert-butylphenol is low in production cost, simple to operate, high in safety, environment-friendly and suitable for industrial popularization.

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis LPF421 and application thereof. The bacillus velezensis LPF421 is classified and named as bacillus velezensis LPF421, and is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCCNO: 65977. The bacillus velezensis LPF421 is named as bacillus velezensis LPF421 and is preserved in the Guangdong Microbial Culture Collection Center. The bacillus velezensis LPF421 disclosed by the invention can generate amylase and cellulase, and has an antagonistic effect on a plurality of moulds. In addition, a soybean meal fermentation test further proves that the strain not only can obviously reduce the content of soybean antigen protein, but also can increase the content of acid soluble protein.

The invention belongs to the technical field of biological control, and particularly relates to the technical field of crop stem rot control. The invention provides a bacillus velezensis Gc8, and the preservation number of the bacillus velezensis Gc8 is CGMCC (China General Microbiological Culture Collection Center) No. 28991. The bacillus velezensis Gc8 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 14, 2023, the preservation number of the bacillus velezensis Gc8 is CGMCC No.28991, the center is called CGMCC for short, and the address of the center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides a compound microbial agent, wherein the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67, kitasapora fluorescens pf27, kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27. The compound microbial agent can inhibit spore germination of pathogenic microorganisms, and has the effect of promoting growth of corn while resisting diseases.

The invention discloses a method for producing bacillus velezensis through solid-state fermentation of distiller's grains, and belongs to the technical field of fermentation engineering. The preparation method comprises the following steps: carrying out acid-base modification, enzymolysis and stacking fermentation treatment on white spirit vinasse, adding bran and jerusalem artichoke powder, carrying out steam explosion, and adding corn steep liquor dry powder, urea and other substances to prepare a white spirit vinasse culture medium; and carrying out amplification culture on the bacillus velezensis subjected to activation and seed culture by using a white spirit vinasse culture medium, drying and collecting the bacillus velezensis. Experimental results show that the bacillus velezensis can be efficiently produced by utilizing the fermentation method provided by the invention, and the spore number of the bacillus velezensis is as high as 9.2 * 10 < 10 > cfu / g. The technical method for producing bacillus velezensis through fermentation effectively solves the problem of resource utilization of white spirit vinasse and the problems of high cost and sewage in the liquid fermentation process, and is low in fermentation cost and suitable for industrial production.

The invention discloses a microbial agent and application thereof in preventing and treating rice blast and promoting rice growth, active ingredients of the microbial agent are formed by compounding cold bacillus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO: 33492 and bacillus velezensis with the preservation number of CGMCC NO: 33493, and the microbial agent aims at solving the synergistic problem of preventing and treating rice blast and promoting rice growth. According to the complex microbial inoculant, through the synergistic interaction between the two strains, the metabolite of the complex microbial inoculant can completely inhibit the spore germination of magnaporthe oryzae; meanwhile, the fungicide can degrade cellulose, obviously promote the growth of plant height, fresh weight and dry weight of rice, and realize the integration of disease prevention and growth promotion. The invention further discloses a preparation method of the fungicide and an application method of the fungicide for preventing and treating rice blast and promoting growth in a root irrigation mode in the three-leaf stage of rice. The microbial agent disclosed by the invention has dual functions of efficiently preventing diseases and stably promoting growth, and an efficient and safe biological solution is provided for green and sustainable development of agriculture.

The invention relates to the technical field of microorganisms, and particularly discloses bacillus velezensis DSSR-2, a biocontrol microbial agent and application of the bacillus velezensis DSSR-2, the bacillus velezensis DSSR-2 is preserved in the China General Microbiological Culture Collection Center on July 8, 2024, the preservation number is CGMCC No.31207, and the bacillus velezensis DSSR-2 is classified and named as bacillus velezensis. The bacillus velezensis DSSR-2 provided by the invention has broad-spectrum antibacterial activity and can be used for inhibiting pathogenic bacteria of plant diseases, so that the plant diseases are prevented and treated, and meanwhile, the growth and development of plants are promoted.