430 results about "Bacillus velezensis" patented technology

The invention relates to plant endogenous bacillus velezensis which is capable of preventing and treating plant diseases and insects and has functions of growth promotion and drought resistance, and application thereof. The preservation number of the Bacillus velezensis E6 provided by the invention in the China General Microbiological Culture Collection Center is CGMCC No. 9665. The strain is separated from a plant root, is rapid to grow, can be rapidly and greatly colonized at plant roots and has ecological niche advantages; the strain E6 not only has an efficient prevention and treatment function on plant soil-borne fungal diseases and is capable of promoting growth of plant root systems and improving drought resistance of plants, but also is capable of damaging formation of pathogenic bacterium bacterial biofilms, is free of drug resistance of bacteria, has relatively good advantages when being compared with other fungicides, is simple in nutrition requirement and low in productioncost, and has good application prospects.

Compositions and methods include a new strain of Bacillus velezensis having growth promoting activity and activity against plant pathogens. The compositions are useful for benefiting plant growth and / or conferring protection against a pathogenic infection when applied to plant foliage, flowers, fruits, bark, roots, seeds, callus tissue, grafts, cuttings, surrounding soil or growth medium, and soil or growth medium concomitant with sowing seed and planting callus tissue, grafts, and cuttings. The compositions containing the Bacillus velezensis RTI301 strain can be applied alone or in combination with other microbial, biological, or chemical insecticides, fungicides, nematicides, bacteriocides, herbicides, plant extracts, plant growth regulators, and fertilizers. In one example, the Bacillus velezensis RTI301 strain can be delivered to the plant as part of an integrated pest management program, with other microbial or chemical insecticides, fungicides, nematicides, bacteriocides, herbicides, plant extracts, and plant growth regulators.

The invention discloses a Bacillus velezensis CGMCC (China General Microbiological Culture Collection Center) No.4992 and application thereof in tomato blight antagonism bacteria. A farmland soil sample on which tomatoes are planted for years in Xinjiang Fukang is subjected to antagonism bacteria screening to obtain a strain with stronger bacteriostatic activity; the strain is further screened and domesticated and bred; and the obtained strain is subjected to morphological specificity and 16S rRNA (Ribose Nucleic Acid) sequencing and system development analysis. The antagonism of the fermentation broth of the strain has outstanding technical effect on solving the tomato blight antagonism bacteria and has wide application field.

The invention discloses a bacillus velezensis strain JN5 and an application thereof. The bacillus velezensis strain JN5 is registered in CGMCC on 13th, December, 2016, the preservation number being CGMCC No.13455. The bacillus velezensis strain JN5 provided by the invention can be prepared into an aqueous agent or a coating agent to be acted on crops such as corn and rice for preventing bacterial stripe of rice and bacterial blight of rice and promoting growth of corn and rice. The strain is safe to use and free of residues, the dosage and residual amount of a chemical agent in an agricultural product are favorably reduced, the production cost is lowered, and the strain is high in practicality and suitable for industrial production.

A marine-derived Bacillus velezensis (Bacillus velezensis) BMF 03 having a deposit number of CCTCC NO: 2017374 is disclosed. BMF 03 strain and its aseptic fermentation broth have inhibitory effect onplant pathogenic fungi, including apple rot fungi, grape white rot fungi, mango anthracnose fungi and so on. The aseptic fermentation broth of BMF 03 strain can be used as an active ingredient to prepare an antibacterial drug, and the bacteria is selected from the group consisting of Keratinobacteria, Bacillus subtilis and Ralstonia solanacearum. The invention also discloses a fermentation methodof aseptic fermentation broth of BMF 03. The invention adopts a plate confrontation method and an Oxford cup method to screen a new marine bacterial strain BMF-03 with strong bacteriostatic effect andwide bacteriostatic spectrum, and adopts strain morphological observation, physiological and biochemical tests and 16S rDNA sequence analysis to determine the species relationship of excellent resistant strains. At that same time, the bacteriostatic substance produce by the strain, the sporulation fermentation medium and the shake flask fermentation conditions were also study, and the promoting effect of the strain fermentation broth and the solid fermentation product on the growth of the cucumber seedling was clarified, which laid a foundation for the application of the strain.

The invention relates to the technical field of agricultural biologics and in particular relates to Bacillus velezensis ANSB01E CGMCC No. 17328 and application thereof. The Bacillus velezensis provided by the invention is capable of simultaneously and efficiently degrading zearalenone and aflatoxin, and degradation rates upon g zearalenone and aflatoxin are both up to 90% or greater 48 hours laterafter reactions. The Bacillus velezensis provided by the invention is high in toxin degradation efficiency, good in specificity, mild in action effect, free of damage to nutrient components in feedsand applicable to biological detoxification of mycotoxins in cereals, feeds and foods.

The invention belongs to the technical field of agricultural biocontrol, and especially relates to a broad-spectrum disease resistance bacillus and an application thereof. The biocontrol bacillus is classified and named as Bacillus velezensis, and the preservation number is CGMCC. No.15766. The Bacillus velezensis has the advantages of broad antibacterial spectrum, strong antagonistic effect, longlasting period, and effectiveness in inhibition of the growth of crop pathogenic fungi, and can be used in the fields of agricultural biocontrol to prevent and control crop diseases caused by gray mold, blight, anthracnose, stem rot, seedling blight, stalk break, gibberellic disease and other fungi. The Bacillus velezensis can also be used for promoting the growth of crop seeds and seedlings andthe preservation and fresh-keeping of fruits and vegetables. The Bacillus velezensis has the characteristics of strong resistance to ultraviolet and drought, simple preparation method and low cost, isa biocontrol strain with excellent performance, and has good development and application prospects.

The invention discloses bacillus velezensis ZF2 and application thereof to control of plant diseases, and provides the Bacillus velezensis (Bacillus velezensis ) ZF2 with the preservation number of CGMCC NO.16013. The invention further relates to the bacillus, or a culture solution or bacillus suspension thereof or application of fermentation products of the bacillus to restrain bacteria. The bacterial strain ZF2 is simple in culture condition in the fermentation process, and massive active substances are generated, are secreted outside cells, and have remarkable antagonism effects on 6 disease fungi and 7 pathogenic bacteria. During pot culture test in green houses, the prevention effect of the bacterial strain ZF2 on corynespora cassiicola is as high as 92.24%, test proves that the bacillus velezensis ZF2 has good application prospects and also provides a certain theoretical basis and technical support for development and application of microbial inoculum.

The invention discloses a bacillus velezensis strain CSQXDZ26, which is bacillus velezensis (Bacillus velezensis) with the preservation number of CGMCC NO. 17935. The bacillus velezensis CSQXDZ26 hasremarkable antagonistic activity on bacterial plant diseases such as Xanthomonas oryzae pv. Oryzae, Xanthomonas oryzae pv. oryzicola, and xanthomonasaxonopodis pv. glycines AND THE LIKE; meanwhile, the bacillus velezensis CSQXDZ26 also has antibacterial activity on fungal plant diseases such as dithiorella gregaria, Fusarium graminearum, Fusarium oxysporum f.sp.Cucumerinum, Fusarium oxysporum f.sp. momordicae, Alternaria solani or Alternaria alternate, and also has excellent inhibition effect on common food spoilage bacteria such as bacillus subtilis, proteusbacillus vulgaris and staphylococcus aureus.

The present invention provides Bacillus velezensis for control of cucumber fusarium wilt and application thereof. The Bacillus velezensis is coded as FJ17-4, and the registration number is CGMCC No. 14642 in the China General Microbiological Culture Collection Center. The Bacillus velezensis FJ17-4 has a significant antagonism effect on cucumber fusarium wilt-Fusarium oxysporum f.sp cucumerinum, and can effectively control cucumber fusarium wilt, and the control effect is stable. The Bacillus velezensis provides an excellent bacterial strain resource for biological control of the cucumber fusarium wilt.

The invention provides bacillus velezensis and application of the bacillus velezensis as an aquatic pathogenic bacteria inhibitor. The bacillus velezensis is separated from the intestinal canal of healthy tilapia, and the preservation number of the bacterial strain is GDMCC No. 60344. The bacillus velezensis has a broad-spectrum antagonistic aquatic common pathogenic bacteria function, and has theadvantages that the growth of the pathogenic bacteria, such as streptococcus agalactiae, streptococcus iniae,nocardia seriolea, aeromonas hydrophila, aeromonas schubertii and edwardsiella tarda, canbe effectively inhibited, the use is safe, no residue is generated, reducing the usage amount and the residual amount of chemical drugs in aquaculture is facilitated, the production cost is reduced, and the practicality is strong.

The invention provides application of Bacillus velezensis NZ-4 to promotion of vegetation. The fermentation broth of the bacterial strain NZ-4 can substantially promote the growth of potato plants. The Bacillus velezensis NZ-4 and a microbial strain thereof are of great significance to prevention and treatment of soil borne diseases and successive cropping obstacles in the farmland of China, to sustainable yield increase of grain and to the protection of the ecological environments of farmland.

The invention discloses bacillus velezensis HNt7 with broad-spectrum antibacterial activity as well as a preparation method and application. The antagonistic bacterium bacillus velezensis HNt7 has a number of CCTCC (China Center for Type Culture Collection) M 2018022. The preparation method comprises the following steps: A, selecting a bacillus velezensis HNt7 strain individual bacterial colony under a sterile condition and inoculating into a culture medium; adjusting the temperature and carrying out shake culture; B, filling a fermenting solution into each bottle and adding individual bacterial colony bacterium liquid according to the ratio; putting the bottle into a large-amplitude constant-temprature freezing shaking table and carrying out the shake culture; C, separating the antagonistic bacterium: immersing with alcohol with concentration and rinsing with sterile water; carrying out dark culture in a culture box and transferring a grown bacterial colony into a new culture medium and carrying out lineation separation and purification on the bacterial colony; D, after separating and purifying, obtaining the bacillus velezensis HNt7. The bacterium is used for preparing a medicinefor treating or preventing pear fruit rot disease. The strain has a remarkable disease-prevention effect and the prevention effect reaches 70.76 percent or more. After the bacillus velezensis HNt7 isapplied to pear trees, advanced defoliation of the pear trees is remarkably inhibited and the tree vigor is easy to enhance.

The invention provides bacillus velezensis for inhibiting virus and promoting plant growth and application of a bacillus velezensis SNB55 strain in prevention and treatment of plant virus diseases andplant fungal diseases and promotion of plant growth. The bacillus velezensis for inhibiting virus and promoting plant growth and the application of the bacillus velezensis have the beneficial effectsthat the bacillus velezensis has no toxicity and pathogenicity, is safe to human and livestock, is environment-friendly, and has high stress resistance and stable antibacterial activity; the bacillusvelezensis can inhibit phytopathogens such as bacillus cereus, tobacco mosaic virus, tomato yellow leaf curl virus, fusarium wilt of cucumbers, tobacco target spot pathogens, phytophthora capsici, rice sheath blight disease and botrytis cinerea, can be secrete indoleacetic acid to promote seed germination and plant growth; and the strain is a multifunctional biocontrol bacterium and has a wide application prospect.

The invention discloses bacillus velezensis and an application thereof to preventing and treating tomato phytophthora root rot. The invention provides bacillus velezensis (bacillus velezensis) ZF50, and the preservation number of the bacillus velezensis in the China General Microbiological Culture Collection Center is CMCC No. 16775. The invention provides an efficient biocontrol strain for preventing and treating the tomato phytophthora root rot, and bacillus velezensis has important production significance for a green prevention and control technique of tomato diseases and promotion of sustainable development of the tomato industry.

The invention provides bacillus velezensis as well as a separation screening method and application thereof, and belongs to the field of microbe technology and food processing. The invention providesthe bacillus velezensis with high fermentation capability; the bacillus velezensis is obtained through screening; during the fermentation, the capability of high yielding protease, amylase and fermented bean plasmin is realized; the bacillus velezensis can be applied to food fermentation in a large scale when being prepared into a food fermentation strain; fermentation food obtained through fermentation by using the bacillus velezensis, the contents of free amino acid and douchi fibrinolytic enzyme are high; the nutrition is rich; the characteristics of easy digestion, thrombus prevention andthe like are realized.

The invention relates to the field of biological prevention and control of banana diseases, in particular to a banana fusarium wilt antagonistic strain, a bio-organic fertilizer, a preparation method and application thereof. The banana fusarium wilt antagonistic strain is preserved in China General Microbiological Culture Collection Center on January 9th, 2017, the preservation number is CGMCC NO.13549, and the preservation name is Bacillus velezensis H-6. According to the invention, a banana fusarium wilt pathogenic bacteria 4# physiological race is adopted as an indicating bacterium from healthy banana rhizosphere soil of an onset banana garden, flat preliminary screening and secondary screening are carried out to obtain the Bacillus velezensis H-6 with strong flat inhibition effect on banana fusarium wilt pathogenic bacteria, the Bacillus velezensis H-6 can be prepared into a bio-organic fertilizer for prevention and control of banana fusarium wilt, and further study on the strain in improving the prevention and control effect of biocontrol bacteria can enrich the existing fusarium wilt biocontrol bacteria resource pool.

The invention relates to a biological control agent, a bioorganic fertilizer, and applications thereof. The biological control agent comprises Bacillus velezensis, Bacillus subtilis, Paenibacillus polymyxa, and Bacillus amyloliquefaciens. The provided biological control agent and bioorganic fertilizer can continuously inhibit fusarium oxysporum for a long term, and has a long-lasting control effect on plant blight, in particular, banana blight.

The invention discloses Bacillus velezensis JS25R and an application thereof to biological control of plant diseases caused by fusarium. The strain JS25R is separated from diseased wheat ears, the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.14240, and the strain not only can efficiently inhibit fusarium graminearum when cultivated on a plate, but also has the control effect as high as 77.4% in a greenhouse experiment. As a biological control material for fusarium head blight of wheat and maize, the strain has good application prospect for development of novel biopesticides or biological control microbial agents.

The invention discloses identification and application of a biocontrol bacillus strain, and belongs to the technical field of agricultural microorganisms. Biocontrol bacillus is bacillus velezensis YJC-9, the strain is already reserved in The China Typical Culture Collection Center of Wuhan university at 11thApril in 2018, and the preservation serial number of the strain is CCTCC NO.M 2018196. Thebiocontrol bacillus strain can prevent and control wheat sharp eyespot, wheat scab, sclerotinia rot of colza, gray mold of strawberries, pepper root rot, kidney bean blight and tobacco alternariaalternata, is a biocontrol bacterium having broad-spectrum resistance, and has good application prospects in agricultural disease prevention and control.

The invention discloses bacillus velezensis (Bacillus velezensis) for preventing and treating rice blast and the application of the bacillus velezensis, and belongs to the technical field of microorganisms. The bacillus velezensis (Bacillus velezensis) E69 disclosed by the invention is preserved in China General Microbiological Culture Collection Center (CGMCC) of China Committee for Culture Collection of Microorganisms, the preservation date is 5 September, 2018, and the preservation number is CGMCC NO.16427. The bacillus velezensis E69 provided by the invention can prevent and treat rice blast of rice, the new field of application of the bacillus velezensis is opened up, and the bacillus velezensis has good preventing effects on the rice blast of the rice, has significant bacterium restraining effects on various plant pathogenic bacteria, is high in preventing and treating efficiency, has good effects of preventing and treating the rice blast of the rice having different varieties inthe south and the north of China, and can reduce the yield loss of rice industry caused by plant diseases which arise suddenly near the collection period of the rice to a larger extent.

The invention discloses bacillus velezensis strain HN03 and application thereof. The bacillus velezensis strain HN03 has the accession number CCTCC M 2018084. The pure bacillus velezensis strain HN03has a good inhibitory effect on banana fusarium No. 1 and No. 4 physiological strains, and various other pathogenic bacteria.

The invention discloses a bacillus velezensis TK2019 microbial agent and application thereof. The bacillus velezensis TK2019 is separated from rhizosphere soil of the cotton field of the 44 regiment in the Tumxuk city of Xinjiang, and the bacillus velezensis TK2019 microbial agent is used for preparing the bacillus velezensis TK2019. When the microbial agent is applied to prevention and treatmentof cotton verticillium wilt, cotton fusarium wilt, botrytis cinerea and red date black spots, the inhibition rate of the strain TK2019 on verticillium dahliae of cotton reaches 94.37 percent, the inhibition rate of the strain TK2019 on four tested pathogenic fungi is 84.83-93.73 percent, and the stock solution and 50-time diluted solution of the bacillus velezensis TK2019 microbial agent have thehighest prevention effects which are 92.86 percent and 91.97 percent respectively. Field tests prove that the prevention effect the TK2019 microbial agent on cotton verticillium wilt is 92.69 percent,which indicates that the Bacillus velezensis TK2019 and the microbial agent thereof provided by the invention have the advantages of high efficiency, wide bactericidal spectrum and the like and havewide values for expanding the application field of microbial strains.

The invention discloses a bacillus velezensis ZJ20 strain and liquid preparations thereof. The bacillus velezensis ZJ20 strain is preserved in China general microbiological culture collection center in Chaoyang district, Beijing, China under the number of CGMCC NO.5995 on April 13 2012. A microbial biocontrol agent, namely the novel bacillus velezensis ZJ20 strain, prepared by screening has endospores with high stress resistance and long shelf life, is highly environmentally adaptive and can be produced into commercial biological agents. Metabolites of the bacillus velezensis ZJ20 strain have high bactericidal power, have high colonization ability and fast speed on plants, and can occupy the surface of hybrid bamboo effectively to prevent dieback. Antibacterial protein (chitin with protein) produced by the strain is antibacterial, can promote growth of hybrid bamboo and various types of bamboo branches and leaves, and have a novel effect of recovering posterior production capacity.

The invention relates to a strain of biocontrol bacillus velezensis which is named as TB1501. The strain is preserved in China Microbiological Culture Preservation Management Committee General Microbiological Culture Collection Center, and has the preservation number as CGMCC No.16069 on 6th July, 2018. The strain has a remarkable effect on preventing cucumber powdery mildew. The typical control effect result shows that the TB1501 strain achieves a favorable preventing effect during the process of preventing cucumber powdery mildew, the prevention rate reaches up to 85 percent or more, and thebiocontrol bacillus velezensis is superior to chemical agent tebuconazole and is almost equivalent to novel drug flutriafol.

The invention provides Bacillus velezensis with the preservation number of CGMCC No.19215. The Bacillus velezensis and fermentation liquor and metabolites thereof can be used for inhibiting phytopathogen and promoting plant growth. The Bacillus velezensis has the biocontrol and growth-promoting effects, can effectively reduce soil-borne diseases of crops such as tomatoes, strawberries and corn, and reduces the usage amount of chemical agents. A preparation prepared from the Bacillus velezensis is stable in storage performance and good in control effect, can be in direct contact with crop rootsystems, has no adverse effect on plant growth and development, and is convenient to process and low in production and transportation cost.

The invention belongs to the field of agricultural microorganisms, and discloses a bacillus velezensis CY30 and an application thereof. The bacillus velezensis is bacillus velezensis CY30, and a preservation number is CCTCC NO: M2018710. The strain is reported for the first time to be used for preventing and treating pestalotiopsis theae of tea trees by foliage spray, root irrigation has the effects of promoting growth and increasing yield of the tea trees, and the strain has a wide application prospect in agricultural production.

The invention relates to the technical field of biological prevention and control, in particular to bacillus velezensis L1-21 and application thereof in preventing Huanglongbing. The bacillus velezensis L1-21 is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC NO.15726, and the category is named as bacillus velezensis L1-21. The bacillus velezensis L1-21 of the invention has the advantages of high stress resistance, simple nutrition, high reproduction speed, large effective live bacteria number, stable performance and long storage period. The bacillus velezensis L1-21 can prevent the plant disease due to the fact that the bacillus velezensis L1-21 can produce various antibacterial substances, which comprise various compoundssuch as lipopeptides, polyketides and the like, and the antibacterial substances can inhibit the normal growth of pathogenic fungus and bacteria, can kill the pathogenic fungus and bacteria and can play an effect of preventing and controlling the Huanglongbing.

The invention belongs to the technical field of microorganisms and particularly relates to a bacterial strain capable of antagonizing botrytis cinerea pathogens after picking of fruits and vegetablesand application of the bacterial strain. The antagonistic bacterium is Bacillus velezensis H1, collected in the China Center for Type Culture Collection (CCTCC) with the collection number of CCTCC NO:M 2019274, is separated from pear fruit surfaces, has very significantly effects of inhibiting botrytis cinerea after picking of fruits and vegetables, has higher inhibition actions on botrytis cinerea, can remarkably inhibit germination of botrytis cinerea spores and growth of germ tubes, has good inhibition functions on the botrytis cinerea after picking of pears, natural rotting after picking of winter jujubes and natural l rotting after picking of grapes, and provides an environmental-friendly, simple and effective way for preservation of fruits and vegetables after picking.

The invention provides an antagonistic bacterium NZ-4 for wilt of potatoes. The antagonistic bacterium NZ-4 is Bacillus velezensis, with an accession number of CGMCC No. 13606. The invention also provides the application of the antagonistic bacterium NZ-4 to prevention and treatment of soil-borne diseases of crops. Pot experiment results show that the antagonistic bacterium NZ-4 has significant prevention and treatment effect on wilt of potatoes, with prevention and treatment effect being 62.14%. In addition, the strain NZ-4 also has strong antagonistic effect on pathogens leading to anthracnose of scab potatoes, root rot of wheat, etc. The application of the fermentation broth of the antagonistic bacterium NZ-4 can significantly promote the growth of potato plants. The antagonistic bacterium NZ-4 and microbial agents thereof are of great significance for prevention and treatment of the soil-borne diseases and continuous cropping obstacles of farmland in China, the sustainable yield increase of grain and the protection of farmland ecological environment.