77 results about "Bacillus velezensis" patented technology

This invention belongs to the field of microbial technology, specifically relating to a compound microbial fertilizer that synergistically regulates rhizosphere flora and nitrogen metabolism genes and its application. The microbial agent includes: Bacillus belye (… Bacillus velezensis ), Bacillus subtilis subsp. subtilis ( Bacillus subtilis subsp. subtilis ) and Linyi Streptomyces ( Streptomyces linyiensis Two or three of the following. The microbial agent provided by this invention has significant growth-promoting and nitrogen-fixing effects when applied to crops. It can maintain or even improve soil fertility and crop yield under the condition of reducing nitrogen fertilizer application by 30%, providing a green and efficient new solution for the implementation of "nitrogen reduction and efficiency enhancement".

This invention belongs to the field of complex pollutant removal technology in water bodies, and specifically relates to a microbial composite hydrogel, its preparation method, and its application. The microbial composite hydrogel, by weight, comprises: 1-3 parts polyvinyl alcohol, 3-8 parts polyethylene glycol, 2-6 parts sodium alginate, 0.05-0.08 parts nano-titanium dioxide, 10-25 parts CaCl2, 10-25 parts boric acid, 5-15 parts potassium persulfate, and *Bacillus velezensis*, wherein the concentration of *Bacillus velezensis* is 0.35 × 10⁻⁶. 8 -0.6*10 8 Cells / mL. The microbial composite hydrogel of this invention can effectively treat multiple pollutants in wastewater simultaneously, has a more efficient pollutant degradation capacity and a wider range of applications, and realizes comprehensive integrated treatment of food wastewater.

The application provides bacillus velezensis HNC-17 and an application thereof, and belongs to the technical field of microorganisms. Bacillus velezensis The bacillus velezensis (HNC-17) of the application is preserved in the Guangdong Microbial Digital Culture Collection Center, and the preservation number is GDMCC No:68053. Ralstonia solanacearum The bacillus velezensis HNC-17 with antagonistic effect on ralstonia solanacearum is screened from the rhizosphere soil of cigar tobacco, and the optimal culture medium component and fermentation condition of the strain are optimized and determined. The prevention and treatment effect of the bacillus velezensis HNC-17 of the application on the cigar tobacco bacterial wilt reaches 74.59%, and the growth of cigar tobacco seedlings can be significantly promoted, which lays a foundation for the development of a bacterial agent for the bacterial wilt of cigar tobacco and the green prevention and control of the bacterial wilt.

The invention provides a bacillus velezensis QT1 and an application of the bacillus velezensis QT1. The classification name of the bacillus velezensis is bacillus velezensis QT1, and the bacillus velezensis is preserved in the China Center for Type Culture Collection on November 12, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20252531. The strain shows remarkable biological control efficiency, realizes effective control on various plant pathogens, and can prevent and treat tomato bacterial wilt and tea tree anthracnose. In addition, the bacillus velezensis QT1 can also effectively kill root-knot nematodes (especially meloidogyne incognita). When the strain is applied to plant cultivation, the growth of various pathogens can be remarkably inhibited, the health and yield of crops are improved, the strain is environment-friendly, the use of chemical pesticides is reduced, soil pollution is avoided, and the development requirement of sustainable agriculture is met.

The application provides bacillus velezensis LW56-4 and application thereof. The bacillus velezensis is bacillus velezensis LW56-4, the classification name is Bacillus velezensis , and the preservation address is China.Wuhan.Wuhan University, and the preservation number is CCTCC NO: M 20251875. The bacillus velezensis LW56-4 has broad-spectrum antibacterial property, can reduce animal diarrhea, promote animal gastrointestinal development and improve antioxidant capacity, can effectively promote feed conversion and promote animal growth, and is an ideal substitute for aureomycin and other antibiotics.

The Bacillus velezensis M173 was deposited with the Guangdong Microbial Culture Collection Center with a deposit number of GDMCC No. 61434. After being prepared into a seed-coating agent, Bacillus velezensis M173 can promote the growth and germination of plants and significantly improve the control effect on pathogens. Moreover, Bacillus velezensis M173 can relieve the condition of dead seedlings of crops at seedling stage after being applied to the crops. In particular, both pot experiments and field experiments prove that the Bacillus velezensis M173 of the present invention has a significant control effect on fungal and bacterial diseases, in particular, bacterial wilt and stem rot. Therefore, the Bacillus velezensis M173 of the present invention has broad application prospects.

The invention relates to biotechnology, namely to a process for cultivating theBacillus velezensisbacterial strain, which can be used in the field of medicine for producing protein preparations, a source of bioactive peptides.The process for cultivating theBacillus velezensisCNMN-BB-10 bacterial strain, according to the invention, includes inoculating the bacterial suspension in a concentration of (2.0±0.2)·102CFU / mL in a medium containing, g / L: peptone 19.5, soluble starch 1.5 and distilled water the rest, or casein peptone 10.0, yeast extract 5.0, NaCl 10.0 and distilled water the rest, and cultivating on a rotary shaker at a speed of 200 rpm, for 48 hours, at a temperature of 30±1°C, with subsequent separation of the biomass from the culture liquid by centrifugation at 6000 rpm, for 20 min.The result of the invention consists in increasing the content of synthesized proteins in the culture liquid by 3.21-5.70 times.

The present application relates to the technical field of fermentation engineering, and particularly relates to a method for producing organic fertilizer by thermophilic fermentation of mixed compost of rotten citrus fruits and mushroom residue. The method comprises the following steps: (1) crushing and mixing rotten citrus fruits and mushroom residue, adjusting the initial moisture content to 60-65%, and adding lime to obtain mixed materials; (2) adding bacillus velezensis bacterial liquid to the mixed materials obtained in step (1) for composting; (3) when the temperature of the composting pile drops to 35 DEG C, adding coprinus comatus liquid for further composting. The present application uses rotten citrus fruits and mushroom residue as raw materials, and realizes efficient and collaborative resource utilization of the two types of agricultural waste by optimizing the ratio and inoculating exogenous bacterial agents in stages. The method can solve the environmental pollution problem caused by the discarding of rotten fruits in citrus-producing areas from the source, and provides a high-value outlet for the by-products of the mushroom industry.

The application discloses a novel preparation for inhibiting botrytis cinerea and application thereof, and relates to the technical field of bacteriostatic drugs, and has the technical scheme as follows: the active ingredient of the preparation is composed of isoeugenol and bacillus velezensis fermentation liquor.The half bacteriostatic concentration EC50 value of the novel preparation reaches 59.7 ug / mL, and the bacteriostatic capacity is higher than that of the existing technology of bacto in the same concentration.Based on the influence on the membrane permeability of filamentous fungi, the application can synergistically inhibit the pathogenic activity of postharvest fungi of fruits, and is expected to be used for preparing a novel preparation for preventing and treating high-sugar fruits after harvesting.The application is simple to use, environment-friendly, can be directly smeared on the surface of fruits in the planting stage, can not be directly contacted with the fruits in the storage period, and can provide the bacteriostatic capacity for the storage period by using the fumigation mode.The application provides a new choice for the biological prevention of fruits, and has good economic benefits and social benefits.

The present application relates to the technical field of microbial degradation of rhizoma polygoni multifidi residues, and discloses a composite microbial agent and a preparation method and application thereof.The composite microbial agent contains trichoderma reesei, aspergillus niger, trichoderma longibrachiatum, bacillus amyloliquefaciens, bacillus velezensis and bacillus subtilis; wherein the proportion of the spores or spores of the trichoderma reesei, aspergillus niger, trichoderma longibrachiatum, bacillus amyloliquefaciens, bacillus velezensis and bacillus subtilis in the composite microbial agent is 1-6:1-6:1-6:1-6:1-6:1-6:1-6.Based on this, the composite microbial agent has good degradation efficiency on rhizoma polygoni multifidi residues, can accelerate the composting process of rhizoma polygoni multifidi residues, shorten the composting period of rhizoma polygoni multifidi residues, and the composting product after composting is stable in quality and is a high-quality organic fertilizer, so that effective utilization of resources is realized.

This invention belongs to the field of agricultural microbiology technology and relates to a strain of Bacillus velezensis S-34 and its application in controlling mango leaf anthracnose. It was isolated from the rhizosphere soil of the anthracnose-resistant mango variety 'Jinhuang' and deposited at the China Center for Type Culture Collection (CCTCC) on May 29, 2025, with accession number CCTCC No. M 20251225. The Bacillus velezensis S-34 provided by this invention not only controls mango leaf anthracnose but also exhibits good antagonistic effects against various mango leaf pathogenic fungi. Furthermore, Bacillus velezensis S-34, its sterile fermentation broth, and volatile gases can inhibit the germination and epidermal penetration of Anthracnose spores, thereby inhibiting the infection process of Anthracnose spores on plants. This invention provides a new biocontrol agent and preparation method for the efficient control of mango leaf anthracnose and other foliar diseases in agricultural production, which is beneficial to the green and healthy development of the mango industry.

This invention discloses a highly acid-resistant Bacillus belye strain LYBV022-Ac R This invention relates to a method for screening and acclimating bacteria under progressive acid stress, and its application, belonging to the technical field of industrial microbial breeding and probiotic application. The disclosed high-acid-resistant Bacillus belyeis LYBV022-Ac... R By designing a pH gradient decreasing (6.5→3.0) acclimatization strategy and combining it with microplate rapid screening technology, a breakthrough improvement in acid resistance was achieved. After exposure to a simulated gastric acid environment (pH 3.0, containing pepsin) for 4 hours, the survival rate reached 48.52%, a 970-fold increase compared to the original strain (0.05%), while maintaining excellent growth performance and genetic stability. This invention also provides the application of this strain in the preparation of oral probiotic preparations for livestock and poultry, solving the common technical problem of insufficient potency in oral Bacillus preparations due to gastric acid damage.

The application belongs to the field of agricultural microorganism technology, and particularly relates to a bacillus velezensis with double effects of disease resistance and growth promotion and application thereof. The strain number is BVZX-1. The bacillus velezensis BVZX-1 strain has been preserved in the China General Microbiological Culture Collection Center, the preservation date is November 7, 2025, the preservation address is No. 1, Xili, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 36546. The bacillus velezensis BVZX-1 has effects of disease resistance, growth promotion, seed germination promotion and seed aging delay.

The application discloses a bacillus velezensis agent with multiple tobacco fungal disease prevention and preparation method and application thereof. The application discloses a bacillus velezensis strain with multiple tobacco fungal disease prevention, wherein the preservation name of the bacillus velezensis strain with multiple tobacco fungal disease prevention is Bacillus velezensis YC2177, and the preservation number is CCTCC NO: M20211635. The application can improve production efficiency and product quality, reduce the incidence of fungal diseases, thereby achieving good effects in the process of biological control, preventing and controlling multiple fungal diseases while applying base fertilizer, effectively replacing the use of chemical pesticides, being green and environment-friendly, reducing environmental pollution and improving crop safety.

The application discloses collagen tripeptide and a preparation method and application thereof, and belongs to the technical field of bioengineering. The preparation method comprises the following steps: obtaining bacillus velezensis with high collagenase yield through separation and identification, the bacillus velezensis belongs to probiotics, and high-activity collagenase is obtained through fermentation culture and purification; taking shark cartilage or blue fish skin as raw materials, and obtaining collagen tripeptide through the following steps of combined enzymolysis of alkaline protease and collagenase after pretreatment, inactivation, centrifugal separation and freeze-drying. The collagen tripeptide prepared by the application has a molecular weight of less than 500 Dalton, contains active components such as GHK tripeptide, is easy to absorb and has high safety, and can be used for preparing antioxidant products, anti-inflammatory products, memory-enhancing products or immunity-enhancing products. The application solves the problems of unsafe collagenase source and low collagen tripeptide preparation efficiency, and has a good industrial application prospect.

The application discloses a tobacco source bacillus velezensis chq3-4 and its amylase and application, and belongs to the field of tobacco microorganisms. The bacillus velezensis chq3-4 strain belongs to Bacillus, is classified and named as Bacillus velezensis chq3-4, is preserved in the China Center for Type Culture Collection, the preservation date is June 19, 2024, and the preservation number is CCTCC NO: M 20241305. The amylase crude enzyme solution prepared from the strain has an enzyme activity of 119.28+ / -0.76 U / mL, and the enzyme has strong stability and can still maintain certain enzyme activity under high-temperature and alkaline conditions. The amylase crude enzyme solution is applied to tobacco products, can effectively reduce the content of tobacco starch in the tobacco products, improve the content of reducing sugar, and then improve the quality of the tobacco products.

This invention belongs to the field of microbial technology, specifically relating to a type of endophytic Bacillus belye AN6 in rice and its applications. Specifically, this invention provides a Bacillus belye AN6, classified and named as follows: Bacillus velezensis This invention provides a novel rice endophytic Bacillus bereaves strain AN6. Through isolation, screening, identification, and experimental verification, it was found that this strain exhibits broad-spectrum resistance to various plant pathogens, including rice bacterial blight and rice blast. This strain has the ability to inhibit multiple plant pathogens and adsorb heavy metals, which is of great significance for the biological control of rice diseases and the development of green agriculture. (CCTCC accession number: M 20231886)

This invention belongs to the field of biotechnology. To improve the effectiveness of biological control of plant diseases, this invention discloses *Bacillus velezensis* ZF516 and its application in controlling various soil-borne diseases. The *Bacillus velezensis* strain number is ZF516, and its registration number at the China General Microbiological Culture Collection Center is CGMCC No. 30136. This *Bacillus velezensis* strain can prevent and / or treat tomato bacterial canker, tomato bacterial wilt, or cowpea root rot, or can be used to prepare products for the prevention and / or treatment of these diseases. This strain also exhibits good inhibitory effects against *Fusarium oxysporum*, *Diplostomum spp.*, *Rhizoctonia solani*, *Fusarium solanum*, *Corynebacterium multiflorum*, *Botrytis cinerea*, *Agrobacterium tumefaciens*, *Pectinobacter brasiliensis*, *Ralstonia solanacearum*, *Streptomyces spp.*, etc.

U I T T R E K S E L The present invention belongs to the field of agricultural microbial control technology, and specifically relates to a growth—promoting and disease—preventing compound microbial inoculant, a preparation method and an application thereof. The compound microbial inoculant specifically includes microbial powder, growth auxiliary materials, and. a desiccant. The microorganisms include Talaromyces flavus, Trichoderma harzianum, Bacillus velezensis, Bacillus licheniformis, and Brevibacillus laterosporus. Through the synergistic effect of multiple microbial strains, the compound microbial inoculant of the present invention enhances the control effect against Verticillium wilt pathogens, and overcomes the shortcomings of a single biocontrol microbe, such as a single biocontrol mechanism, poor environmental adaptability, inability to colonize in soil for a long time, and unstable control effect. Furthermore, the compound microbial inoculant of the present invention can increase the yields of cotton and pepper, and improve the fiber quality of cotton. The present invention provides scientific and technological support and decision-making services for realizing green, environmentally friendly, cost—saving, and efficiency-increasing modern agriculture.

This invention discloses a strain of *Bacillus belyssus* S2 and its application in the control of early blight in tomato, belonging to the field of microbial technology. *Bacillus belyssus* S2 is deposited at the China Center for Type Culture Collection (CCTCC) on March 30, 2026, with accession number CCTCC NO: M 2026551. The *Bacillus belyssus* S2 isolated and screened in this invention can secrete siderophores, cellulases, proteases, 1,2-β-glucanases, and lipases. Its soluble and volatile substances can inhibit the mycelial growth of tomato pathogens and cause mycelial malformation, reduce spore germination rate, delay the onset of early blight in tomato fruits, and enhance the fruit's resistance, providing a new microbial resource for the control of diseases such as early blight in tomato.

This invention belongs to the field of microbial preparation technology, specifically relating to a biocontrol agent composition and its application in improving growth barriers caused by high-temperature soil sterilization and controlling plant diseases. The technical problem this invention aims to solve is to provide a new option for alleviating soil obstacles caused by high-temperature sterilization. The technical solution of this invention is a *Pseudomonas aeruginosa* 115-17, with the preservation number CCTCC No: M2026293. This invention provides a novel *Pseudomonas aeruginosa* 115-17 strain, which possesses nitrogen-fixing, inorganic phosphorus-solubilizing, and siderophore-producing abilities; it can also promote pepper growth, control plant diseases (mainly pepper blight), and improve plant growth inhibition caused by high-temperature soil sterilization. This invention also provides a combination of Bacillus velezensis Bv-6 (CCTCC No: M20191106) and newly isolated Pseudomonas aeruginosa 115-17 to form a microbial inoculum, thereby effectively alleviating plant growth obstacles caused by high-temperature sterilization and effectively preventing and controlling pepper blight.

This invention relates to the field of biotechnology, specifically to *Bacillus belyssiensis* NH4 / 2 and its applications. This invention provides *Bacillus belyssiensis* NH4 / 2 and its applications. The *Bacillus belyssiensis* NH4 / 2 provided by this invention exhibits strong broad-spectrum inhibitory effects against pathogens such as bacterial wilt, red spot pathogen, tobacco black shank pathogen, anthracnose pathogen, wilt pathogen, and gray mold pathogen. Furthermore, it is isolated from soil and is harmless to humans, livestock, and the environment, meeting the needs of green plant protection and sustainable agricultural development.

This invention discloses a strain of *Bacillus belyceae* with broad-spectrum antibacterial activity and its application in the prevention and treatment of necrotic enteritis in poultry. The *Bacillus belyceae* strain provided by this invention (… Bacillus from Velez The genome of strain GDG25CB056 is approximately 3.91 Mb in size, contains no virulence factors, and is rich in gene clusters encoding non-ribosomal peptides and polyketide synthases. Studies have confirmed that this strain exhibits significant growth advantages, strong tolerance to high temperatures, gastric acid, and bile salts, which facilitates intestinal colonization. It can produce siderophores and various enzymes, and has overcome the technical bottleneck of a narrow antibacterial spectrum, possessing broad-spectrum antibacterial activity, especially strong specific antagonistic activity against Clostridium perfringens. In vivo experiments have shown that this strain can significantly reduce the Clostridium perfringens load in the cecum of chicks with necrotizing enterocolitis, alleviate intestinal congestion and swelling, repair damaged intestinal villi, and enhance mucosal barrier function, providing a safe and efficient strain resource for the prevention and treatment of bacterial enteric diseases.

The application discloses application of dimethoxyphenol and a biological preparation thereof in plant pathogenic nematode control, and aims to solve the problems of single function, unknown active ingredient, unclear mechanism and unstable quality of existing microbial source preparations. The application accurately identifies 2,3-dimethoxyphenol as an important nematicidal active substance through metabolomics, and realizes stable co-accumulation of 2,3-dimethoxyphenol and a growth-promoting factor, isopentenyladenine, by combining with a specific strain (pink helical polysporal mold NF-06 and bacillus velezensis YB-1652) co-culture process, so that technical effects of clear composition, synergistic disease-preventing and growth-promoting function, controllable quality and environment friendliness are achieved.

The present application relates to a kind of veterinary immunity-enhancing antiviral powder and its preparation method, the veterinary immunity-enhancing antiviral powder includes traditional Chinese medicine fermentation powder 60-70%, phoenix tree seed oil 20-30% and dioscorea opuloides mucopolysaccharide 10-20% according to mass percentage;The traditional Chinese medicine fermentation powder is first obtained fermentation product using bacillus velezensis, Candida utilis fermentation scutellaria baicalensis, astragalus, pomegranate peel, hawthorn and polygonatum sibiricum, then is formed by drying, ultrafine grinding to fermentation product.This application can improve the quality concentration of piglet serum immunoglobulin, so that piglet immunity, antiviral capacity is improved, in addition, it has good therapeutic effect on piglet viral gastroenteritis.

The invention relates to biotechnology, namely to a process for cultivating theBacillus velezensisbacterial strain, which can be used in the field of medicine for producing protein preparations, a source of bioactive peptides.The process for cultivating theBacillus velezensisCNMN-BB-12 bacterial strain, according to the invention, includes inoculating the bacterial suspension in a concentration of (2.0±0.2)·102CFU / mL in a medium containing, g / L: peptone 19.5, soluble starch 1.5 and distilled water the rest, or casein peptone 10.0, yeast extract 5.0, NaCl 10.0 and distilled water the rest, and cultivating on a rotary shaker at a speed of 200 rpm, for 48 hours, at a temperature of 30±1°C, with subsequent separation of the biomass from the culture liquid by centrifugation at 6000 rpm, for 20 min.The result of the invention consists in increasing the content of synthesized proteins in the culture liquid by 3.69-6.90 times.

The application belongs to the technical field of gene detection, and particularly relates to specific primers, a Taqman probe and application of a bacillus velezensis HMB26553 strain. The specific primers and the probe sequence of the bacillus velezensis HMB26553 strain are respectively SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3. The primers can amplify a single unique target fragment from the bacillus velezensis HMB26553 strain, and realize specific detection of the bacillus velezensis HMB26553 strain. The primers have the characteristics of high specificity, high detection efficiency, low false positive rate, long preservation time and the like. By using the primers and through real-time quantitative PCR technology, the population quantity of the bacillus velezensis HMB26553 strain in the rhizosphere of cotton can be quickly quantified, and the primers have the advantages of high throughput, rapidness and accuracy.

The application relates to the technical field of microorganisms, and particularly discloses a bacillus velezensis HP-BL2 and application thereof. Bacillus licheniformis HP- B2 The strain is preserved in the China Center for Type Culture Collection, is located in Wuhan, Wuhan University, China, has a preservation number of CCTCC NO: M20242677, and was preserved on November 29, 2024. The application also provides application of the bacillus velezensis HP-BL2 in feed, improves the nutritional value of the feed, promotes the growth of poultry and livestock, regulates intestinal microecology, reduces intestinal diseases, improves the feed digestion rate, and enhances the immunity of the body.

The application discloses application of bacillus velezensis LH-BLS107 (CGMCC No. 34785) or an engineering bacterium modified through natural, artificial mutagenesis or gene editing in biological control of plant nematode diseases. Fermentation culture solution, bacterium powder and / or secondary metabolites of the LH-BLS107 or the engineering bacterium thereof are used in wheat, soybean, rice, fruit trees, vegetables and the like. The fermentation culture solution, the bacterium powder and / or the secondary metabolites can effectively prevent and control wheat cyst nematode disease, soybean cyst nematode disease, rice paratylenchus pseudohollandei disease, fruit tree and vegetable root knot nematode disease, and the mortality of the above-mentioned nematodes can reach more than 95%, and when the fermentation culture solution, the bacterium powder and / or the secondary metabolites are compounded with chemical nematocide, the chemical agent dosage can be reduced by 25%-30%.

The present application discloses bacillus velezensis strain 1-1-6 and bacillus composticola strain 2-1-3 and a compound microbial inoculant and use thereof. The two strains have a significant inhibitory effect on the fusarium of the Morchella esculenta stem rot pathogen, and have a synergistic antagonistic effect when combined. The two strains are fermented together to form an inoculant, and the inoculant is sprayed on the surface of the Morchella esculenta cultivation compartment at a rate of 500 mL / m 2 The incidence of stem rot can be significantly reduced by spraying the inoculant on the surface of the Morchella esculenta cultivation compartment, providing a safe and effective microbial inoculant for the biological control of Morchella esculenta stem rot.