445 results about "Bacillus velezensis" patented technology

The invention belongs to the technical field of microorganisms, particularly relates to rice endophytic bacillus velezensis AN6 and application thereof, and particularly provides bacillus velezensis AN6 which is classified and named as bacillus velezensis and has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20231886. The invention provides a novel rice endophytic bacillus velezensis strain AN6, through separation, screening, identification and experimental verification, it is found that the strain shows broad-spectrum resistance to various phytopathogens including rice bacterial leaf blight, rice blast and the like, the strain has the capacity of inhibiting various phytopathogens and has the capacity of adsorbing heavy metal, and the strain has the advantages of being capable of inhibiting various phytopathogens and capable of being used as a novel rice endophytic bacillus velezensis strain AN6. The method is of great significance to biological control of rice diseases and development of green agriculture.

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention belongs to the technical field of agriculture, and particularly provides a compound microbial agent and application thereof. The compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02. The compound microbial agent can effectively improve the pH and EC of saline alkali soil, increase the content of alkali-hydrolyzable nitrogen, available phosphorus and quick-acting potassium in the soil, and play a significant role in increasing the plant height, stem diameter, chlorophyll content and aboveground biomass of corn plants in coastal saline-alkali soil.

The invention discloses a microbial agent and application thereof in preventing and treating rice blast and promoting rice growth, active ingredients of the microbial agent are formed by compounding cold bacillus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO: 33492 and bacillus velezensis with the preservation number of CGMCC NO: 33493, and the microbial agent aims at solving the synergistic problem of preventing and treating rice blast and promoting rice growth. According to the complex microbial inoculant, through the synergistic interaction between the two strains, the metabolite of the complex microbial inoculant can completely inhibit the spore germination of magnaporthe oryzae; meanwhile, the fungicide can degrade cellulose, obviously promote the growth of plant height, fresh weight and dry weight of rice, and realize the integration of disease prevention and growth promotion. The invention further discloses a preparation method of the fungicide and an application method of the fungicide for preventing and treating rice blast and promoting growth in a root irrigation mode in the three-leaf stage of rice. The microbial agent disclosed by the invention has dual functions of efficiently preventing diseases and stably promoting growth, and an efficient and safe biological solution is provided for green and sustainable development of agriculture.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A118 and application thereof. The bacillus velezensis A118 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on colletotrichum capsici. The bacillus velezensis A118 disclosed by the invention also has relatively strong capability of producing cellulase, amylase, protease and indole-3-acetic acid (IAA) and has a relatively good growth promoting effect on chilies, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in a use process and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of pepper diseases is reduced, the pepper growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention discloses a bacillus strain H7 and an application thereof. The invention provides bacillus velezensis H7 or a progeny of the bacillus velezensis H7, and the preservation number of the bacillus velezensis H7 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M 2025630. The bacillus velezensis H7 disclosed by the invention has a very good antibacterial effect on various common clinical pathogenic bacteria (including klebsiella pneumoniae, escherichia coli, candida albicans, enterobacter cloacae complex, acinetobacter baumannii and the like), and particularly shows a very high inhibition rate on some drug-resistant clinical pathogenic bacteria; therefore, the compound has important application value in development of drug and non-drug antibacterial products (such as medical antibacterial materials and the like), a new choice can be provided for drug and non-drug prevention and control of clinical pathogenic bacterium infection, and particularly, the compound has a supplementary effect in the aspects of reducing drug-resistant bacterium propagation risk and reducing drug dependence.

The invention relates to bacillus for producing lipopeptide, bacteriostatic lipopeptide of the bacillus and application of the bacillus to aquatic products, and belongs to the technical field of microorganisms. The bacillus velezensis is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063, and the bacillus velezensis strain FJAT-57093 is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063. The bacteriostatic lipopeptide is prepared from three types of lipopeptides, namely, iturin, fengycin and surfactin. The invention further discloses a preparation method of the bacteriostatic lipopeptide. The bacillus and the bacteriostatic lipopeptide have a broad-spectrum bacteriostatic effect on aquatic pathogenic bacteria, can be used for aquaculture, and have a wide application prospect.

The invention discloses heavy metal resistant bacillus velezensis as well as a microbial remediation agent and application thereof. Bacillus velezensis DBS01 is screened from heavy metal contaminated soil in Dabaoshan mining areas in Shaoguan City, Guangdong province, is preserved in China Center for Type Culture Collection (CCTCC), has a preservation number of CCTCC M 20252228, not only has resistance to various heavy metals, but also can metabolize and secrete metabolites such as organic acid, cellulase and growth hormone in different growth stages, and can be used for preventing and treating various heavy metals. Multiple functions of curing heavy metals, dissolving phosphorus and potassium, promoting plant growth and the like are realized. According to the method, bacillus velezensis DBS01 is inoculated into a culture medium containing straw, calcium superphosphate, agar and sodium alginate are added, and a mixture containing the bacillus velezensis DBS01 is freeze-dried, so that the heavy metal contaminated soil in the mining area can be efficiently repaired in an environment-friendly manner at low cost, and the ecological function recovery of the soil is effectively promoted.

The invention relates to bacillus velezensis Ab1 with a water mildew inhibiting effect and application of the bacillus velezensis Ab1, the classification name of the bacillus velezensis Ab1 is bacillus velezensis Ab1, the preservation number of the bacillus velezensis Ab1 is CCTCCNO: M 20251457, and the preservation date of the bacillus velezensis Ab1 is June 23, 2025. The bacterium can effectively inhibit the propagation rate of water mildew in a water body, improve and purify the water body environment and improve the culture health degree of aquatic animals, and has a wide application prospect in aquaculture.

The invention provides bacillus velezensis and an application of the bacillus velezensis in degradation of antibiotics. The bacillus velezensis is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the bacillus velezensis is GDMCC No: 66277. The bacillus velezensis is named as bacillus velezensis. The invention also provides a bacillus velezensis preparation or a bacillus velezensis liquid containing the strain. The invention also provides an application of the bacillus velezensis or the microbial inoculum thereof or the bacterial liquid thereof in degradation of kanamycin antibiotics. The application comprises the following steps: degrading the residual kanamycin antibiotics in soil or sewage, or repairing the soil polluted by the residual kanamycin antibiotics.

The invention discloses bacillus velezensis BR-1 and application of the bacillus velezensis BR-1 in prevention and treatment of economic crop diseases. The preservation number of the bacillus velezensis BR-1 is CCTCC NO: M 20251379. The bacillus velezensis BR-1 disclosed by the invention is high in adaptability and wide in growth condition range, and is more beneficial to application in agricultural production. Meanwhile, the bacillus velezensis BR-1 has a remarkable inhibition effect on the growth of various phytopathogens such as verticillium dahliae, fusarium oxysporum, sclerotinia sclerotiorum and ralstonia solanacearum, can remarkably reduce the morbidity of cotton verticillium wilt, fusarium wilt and tomato bacterial wilt under a greenhouse condition, and has a wide agricultural application prospect.

The invention discloses bacillus velezensis B127 and application thereof, the bacillus velezensis is preserved in China Center for Type Microbiological Culture Collection, the preservation number is CCTCC NO: M 20252878, the preservation date is December 15, 2025, the classification name is bacillus velezensis B127, and the preservation address is Wuhan University, Hubei Province. The bacillus velezensis B127 has a remarkable inhibition effect on various tobacco fusarium pathogenic bacteria, has good phosphorus and potassium solubilizing capacity, generates hydrolase related to inhibition of pathogenic bacteria and generates siderophore and indoleacetic acid for promoting plant growth, is a biocontrol bacterium with excellent comprehensive performance, and can be widely applied to the field of biological control. The method has good development and application prospects in the fields of agricultural disease control, soil improvement, microbial fertilizers and the like.

The invention belongs to the technical field of fermentation engineering and microbial disease prevention and control, and particularly relates to application of bacillus velezensis fermentation liquor in prevention and control of citrus red mites. A bacillus velezensis XZT106 strain is subjected to fermentation treatment to obtain fermentation liquor without thalli, the fermentation liquor contains effective components including epothilone A and silthiopham, experiments prove that the death rate of red spiders treated by a stock solution of the fermentation liquor is 100%, the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 200 times and 500 times are 87.8% and 66.7%, and the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 500 times and the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 200 times and 500 times are 87.8% and 66.7% respectively. In addition, it is further verified that the fermentation liquor of the bacillus velezensis XZT106 thallus does not generate the problems of drug resistance and environmental pollution in the process of preventing and treating the red spiders.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A111 and application thereof. The bacillus velezensis A111 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on phytophthora capsici and ralstonia solanacearum. The bacillus velezensis A111 also has relatively strong capability of producing cellulase, amylase, protease, ACC deaminase and indole-3-acetic acid (IAA), and has a relatively good growth promoting effect on tomatoes, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in the use process, and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of tomato diseases is reduced, the tomato growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A96 and application thereof. The bacillus velezensis A96 disclosed by the invention can resist saline and alkaline, and has a good inhibition effect on fusarium oxysporum. In addition, the bacillus velezensis A96 also has relatively strong capacity of producing cellulase, amylase, protease and indole-3-acetic acid (IAA) and has a relatively good growth promoting effect on cowpeas, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in the use process and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the control cost of cowpea diseases is reduced, the growth of cowpeas is promoted, and the biocontrol bacterium has a good popularization value.

The invention provides a composite flora for preventing and treating cotton verticillium wilt and a microbial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The composite flora and the microbial agent provided by the invention comprise pseudoarthrobacter oxydans CRB-17 and bacillus velezensis CRB-46; the preservation numbers of the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are respectively CCTCC (China Center for Type Culture Collection) NO: M20252254 and CCTCC NO: M20252255, and the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are both preserved in the China Center for Type Culture Collection on October 20, 2025. The pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are stably colonized in the rhizosphere of the cotton, have a strong direct antagonism effect on verticillium dahliae, can effectively and continuously prevent and treat the verticillium wilt of the cotton, effectively promote the germination of cotton seeds, the elongation of radicles and hypocotyls, promote the growth of the cotton and improve the defensive power of the cotton.

The application discloses a bacillus velezensis and application of the bacillus velezensis in rapid increase of sauce aroma in salt-reduced soy sauce fermentation, and belongs to the technical field of food fermentation. The bacillus velezensis Bv-M1 is isolated from soy sauce fermentation mash for the first time, the bacillus velezensis strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.32853. Compared with the fermentation soy sauce without the addition of the bacillus velezensis Bv-M1 aroma-enhancing koji immersion liquid, the product has obvious increase of TTMp content and sauce aroma, and currently, there is no reported application example of the bacillus velezensis strain in application of soy sauce fermentation and sauce aroma increase.

The invention belongs to the technical field of biocontrol bacteria, and discloses bacillus velezensis and application thereof in preventing and treating bacterial wilt of solanaceae crops. The bacillus velezensis is named as bacillus velezensis PR-7, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation time is November 13, 2023, and the preservation number is CGMCC No.28979. The bacillus velezensis has the advantages that the bacillus velezensis is named as bacillus velezensis PR-7; the bacillus velezensis can effectively inhibit the growth of a bacterial wilt pathogen, i.e., the evolutionary I type (main evolutionary type of bacterial wilt pathogen in South China) of bacterial wilt pathogen, improves the bacterial wilt resistance of solanaceae crops, and is of great significance to the prevention and treatment of bacterial wilt of solanaceae crops in South China.

The invention relates to the field of microorganisms, in particular to bacillus velezensis and application of the bacillus velezensis in preparation of surfactin, the preservation number is CCTCC NO: M 20251689, the bacillus velezensis is a wild type bacillus velezensis strain, and the surfactin yield of the bacillus velezensis is 2-3 times higher than that of a conventional wild strain. At the shake flask level, the yield of the surfactin reaches 10.4 g / L, when fed-batch fermentation is carried out in a 5L fermentation tank, the yield reaches 22.3 g / L, the yield and production intensity are higher than the currently known wild fungus fermentation level, and the method has huge industrial application potential.

The invention belongs to the technical field of microbial fermentation and insect pest prevention and control, and particularly relates to endogenous bacillus velezensis and application of fermentation liquor of the endogenous bacillus velezensis in prevention and control of bemisia tabaci. The invention discloses bacillus velezensis NM153 which is preserved in the China Center for Type Culture Collection on March 27, 2025, and the preservation number of the bacillus velezensis NM153 is CCTCC (China Center for Type Culture Collection) M 2025605. A stock solution of the bacillus velezensis NM153 supernate and the bacterial suspension is diluted by 300 times and 500 times to treat the bemisia tabaci, and the result shows that the death rate of the bemisia tabaci treated by the bacillus velezensis NM153 supernate is obviously higher than that of a bacterial suspension treatment group, and the death rate of the bemisia tabaci treated by the bacterial suspension treatment group is obviously higher than that of the bemisia tabaci treated by the bacillus velezensis NM153. In addition, the bacillus velezensis NM153 metabolite is further verified and explained to have a remarkable technical effect on preventing and treating bemisia tabaci in o-carboxybenzaldehyde, tenuitoxin and micronomicin in the bacillus velezensis NM153 metabolite.

The invention discloses a bacillus velezensis YP-5 strain and an application thereof. Relates to the technical field of microorganisms. The invention provides a preservation number, a fungicide, a method for improving the planting capacity of bacillus velezensis in tea tree leaves and specific application. The bacillus velezensis YP-5 is high in control effect and wide in control spectrum. In addition, under the technological conditions of the invention, the quantity of colonized bacteria on tea tree leaves is increased by 1.9 times compared with that of a blank control group; after 7 days of treatment, the fixed planting bacteria quantity is 3.42 times of that of a blank control group. Meanwhile, when the microbial agent is sprayed to the tea trees in an indoor environment, the biological control effect on the tea anthracnose can be remarkably enhanced, and the biological control effect is improved by 10.29% compared with that of original YP-5 bacterial liquid. The problem that in the prior art, microbial thalli are weak in adhesion and prone to loss, and consequently the biocontrol capacity of the microbial thalli is weakened is effectively solved, and the method is suitable for industrial application of agricultural microbial preparations.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof in plant disease control. A bacillus velezensis strain is separated and screened from rhizosphere soil of a wolfberry plant in a Nomuliflood farm in Qinghai province, the strain is bacillus velezensis G3 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 36136. The bacterial strain has a better antibacterial effect on pathogenic bacteria fusarium oxysporum of Chinese wolfberry root rot, also has an inhibiting effect on pathogenic bacteria of Chinese wolfberry verticillium wilt, botrytis cinerea, sclerotinia rot of colza, alternaria alternata, apple anthracnose, apple rot, pear wilt and wheat scab, and has broad-spectrum antibacterial activity; wide application prospects are realized in plant disease prevention and treatment.

The invention discloses bacillus velezensis as well as a biological preparation and application thereof, and belongs to the technical field of microorganisms. The bacillus velezensis is rice endophytic bacillus velezensis, is classified and named as bacillus velezensis HA-B13, is preserved in the China Center for Type Culture Collection (CCTCC), has the preservation number of CCTCC NO: M 20251098, shows extremely excellent biological activity to nigatosporium oryzae, has the antibacterial rate of 94%, and can be used for preparing the bacillus velezensis. Meanwhile, the bacillus subtilis also has relatively good biological activity on a plurality of rice pathogenic bacteria such as rhizoctonia solani, pyricularia grisea, gylastoma gonoryzae and the like. And the strain is derived from rice plants and can be well colonized in the rice plants, so that the strain has a long-acting prevention and treatment effect on diseases such as spike rot and the like, can effectively reduce the investment of rice disease prevention and treatment products, is safer and has great prevention and treatment application potential.

The invention discloses bacillus velezensis as well as a compound medicament and application thereof, and belongs to the technical field of plant disease control. In order to solve the problems of easy generation of drug resistance, influence on ecological environment, residue of forbidden pesticide, excessive pesticide and the like in the existing chemical control method for cowpea fusarium wilt, the invention screens out a bacillus velezensis T9 with a relatively good inhibition effect on pathogenic bacteria of cowpea fusarium wilt; experiments find that the strain T9 has an obvious inhibition effect on various plant pathogenic fungi, and a bacterial solution of the strain T9 can effectively prevent and treat cowpea fusarium wilt and can effectively promote cowpea growth; besides, the compound medicament prepared from prochloraz and bacillus velezensis T9 bacterial liquid is used for irrigating cowpea seedlings, and the compound medicament is found to have a good control effect on cowpea fusarium wilt, can effectively reduce the use amount of chemical medicaments, and also has a certain growth promoting effect on cowpea plants.

The invention relates to the technical field of agricultural microorganisms, and discloses stress-tolerant bacillus velezensis L-29 and application thereof. The bacillus velezensis L-29 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35587. The bacillus velezensis L-29 is named as bacillus velezensis L-29. Experiments prove that the bacillus velezensis L-29 can adapt to various extreme environments, can survive and proliferate under the extreme environments of drought conditions simulated by 0-25% PEG 6000 under the conditions that the pH is 2-12 and the salt concentration is 1.5%, and has various functions of soil improvement (organic matter decomposition and phosphorus activation), plant growth promotion, broad-spectrum disease control and the like. In the secondary fermentation stage of compost, humus generation can be remarkably promoted, compost maturation is accelerated, the good colonization ability is achieved, and after an obtained compost product is applied to normal soil, the nutrient effectiveness can be improved, crop growth is promoted, and the disease resistance is enhanced.

The invention relates to the technical field of agricultural plant disease control, and particularly discloses a biological and chemical compound bactericide and application thereof. The biological and chemical compound bactericide comprises a bacterial solution of a biocontrol strain bacillus velezensis LHN1 and a chemical bactericide, the biocontrol strain bacillus velezensis LHN1 is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 63550; the chemical bactericide is selected from at least one of prochloraz, pyraclostrobin or tebuconazole; in the compound bactericide, the volume ratio of the LHN1 bacterial liquid to the chemical bactericide is (1: 4)-(4: 1). Through the synergistic effect of biological prevention and control and chemical prevention and control, the problems that a single chemical bactericide pollutes the environment and the single biological prevention and control effect is unstable are solved, the prevention effect in a pot experiment reaches up to 83.99%, meanwhile, the dosage of chemical agents is reduced by 30%-50%, and the method has the advantages of being efficient, low in toxicity and low in cost and is suitable for root rot prevention and control in large-scale planting of rhizoma polygonati.

The invention belongs to the field of microorganisms, and discloses bacillus velezensis and application thereof in prevention and treatment of plant fungal diseases. The bacillus velezensis disclosed by the invention is bacillus velezensis YC46, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.33975. The bacillus velezensis can grow by taking leftovers in an aquatic product processing process as a unique nutrient source, and has the advantages of high yield, low cost and the like. The bacillus subtilis has the antibacterial activity on plant pathogenic fungi such as fusarium oxysporum, botrytis cinerea, alternaria alternata, colletotrichum gloeosporioides and penicillium, thalli or fermentation products of the thalli can be used as active ingredients for preparing biocontrol microbial agents or bacteriostatic agents for preventing and treating plant fungal diseases, and the bacillus subtilis has good application value and ecological environmental protection value.

The invention relates to a bacillus velezensis strain B12, a fungicide containing the same and application of the bacillus velezensis strain B12. The bacillus velezensis strain B12 disclosed by the invention has an efficient and lasting antagonistic effect on septospora (especially tomato septospora), and can play a key role in biological prevention and control of tomato spot blight. Furthermore, research on endogenous metabolites shows that the volatile metabolite 4-hydroxycoumarin can remarkably improve the effects of antagonizing the tomato Acetrospora chrysosporium and preventing and treating tomato spot blight, so that the 4-hydroxycoumarin can be used for developing a more efficient compound bacterial agent. The bacterial strain and the compound bacterial agent have the characteristics of high efficiency, durability, safety and environmental protection when being used for antagonizing septospora caninum and preventing and treating tomato spot blight caused by septospora caninum, so that the bacterial strain and the compound bacterial agent have relatively high industrial value and application prospect.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof. The strain is separated from saline-alkali soil in the Uygur autonomous region in Xinjiang and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No.65485. By utilizing a response surface optimization method, the cellulase activity reaches 92.376 U / mL and is improved by 95.6% compared with that of a non-optimized cellulase activity; the growth inhibition rate on alternaria solani, alternaria alternate, fusarium oxysporum and botrytis cinerea hyphae is 88.47-91.08%, and the metabolite (especially an ethyl acetate extract) can significantly inhibit Korla pear diseases caused by alternaria alternate. The strain is resistant to high and low temperature, acid and alkali and salt stress, can degrade straw (the degradation rate is 21.9%), and provides a high-quality strain resource for resource utilization of agricultural wastes and biological control of plant fungal diseases.

The invention relates to the technical field of microbial preparations, and particularly discloses bacillus velezensis HY16 fermentation liquor freeze-dried powder and a preparation method and application thereof.The preparation method includes the steps that hydroxyl of beta-cyclodextrin reacts with anhydride of maleic anhydride, double bonds are introduced to beta-cyclodextrin molecules, and then under the action of persulfate serving as an initiator, the beta-cyclodextrin and maleic anhydride react with each other to prepare beta-cyclodextrin HY16 fermentation liquor freeze-dried powder; and carrying out free radical copolymerization reaction on the beta-cyclodextrin and 2-acrylamide-2-methylpropanesulfonic acid to obtain the modified beta-cyclodextrin. Sulfonic acid groups in 2-acrylamide-2-methylpropanesulfonic acid are strong hydrophilic groups, the water solubility of beta-cyclodextrin can be remarkably improved, growth of ice crystals during freezing is delayed, mechanical damage of the ice crystals to strains is reduced, and meanwhile negative charges of the sulfonic acid groups can be effectively absorbed through electrostatic repulsion. The freeze-drying process is simple, aggregation and inactivation of fermentation active substances or strains in the freeze-drying process are prevented, the survival rate of viable bacteria in the freeze-drying powder is increased, the stability of active ingredients in fermentation liquor is kept, and then the algae-lysing effect is improved.