52results about How to "Strong antagonistic effect" patented technology

The invention provides a Trichoderma asperellum TAZ19, a biocontrol inoculant and application of the Trichoderma asperellum TAZ19, the Trichoderma asperellum TAZ19 is preserved in the China Center for Type Culture Collection on October 25, 2024, and the preservation number of the Trichoderma asperellum TAZ19 is CCTCC M 20242341. The active ingredients of the biocontrol inoculant comprise the trichoderma asperellum TAZ19 strain, and the trichoderma asperellum TAZ19 strain is one or more of conidia, hypha, fermentation filtrate or active extracts of the trichoderma asperellum TAZ19 strain of the trichoderma asperellum TAZ19 strain. The trichoderma asperellum TAZ19 has an excellent antagonistic effect on a plurality of plant disease pathogenic bacteria such as dispora pinnatifida, a plurality of fusarium oxysporum, apple black beancurd skin fungi and botryosphaeria dothidea, has broad-spectrum antibacterial activity, is high in growth speed, high in antibacterial activity and high in hyperparasitism ability, has better saline-alkaline tolerance, also has a growth promoting effect on plants, and can be widely applied to the field of plant diseases. The strain can be used as an active component of a biocontrol inoculant to be widely applied to prevention and treatment of various plant diseases such as pine tree branch rot, wheat stem rot and tomato fusarium wilt, and has a wide application prospect.

The invention discloses a precipitation-supported slow-release carbon-based iron fertilizer and a preparation method thereof, and the preparation method specifically comprises the following steps: firstly, putting a biological carapace heavy metal adsorbent into a calcium hydroxide solution for dipping, drying, then putting the biological carapace heavy metal adsorbent into a ferrous sulfate solution for dipping in two times, and finally drying and granulating. Precipitated ferric hydroxide, a ferrous hydroxide mixture and excessive ferric sulfate are uniformly dispersed and attached to the interiors of pore channels of the porous adsorbent, and the excessive ferric sulfate can quickly provide iron ions for antagonistic cadmium and arsenic ion absorption in the early growth stage of rice. Precipitated ferric hydroxide and ferrous hydroxide wrapped by calcium sulfate in the porous adsorbent not only can realize long-acting slow release of iron ions, but also can reduce the oxygen content of soil, and iron ion antagonism and precipitation type adsorption loading modes are carried out in the whole growth process of rice, so that the release period of the iron ions is greatly prolonged, and the soil quality is improved. The iron fertilizer disclosed by the invention has an excellent rice cadmium-arsenic antagonism effect and has an excellent application prospect.

The invention relates to the technical field of microorganisms, in particular to paenibacillus polymyxa ZR-4 and application of the paenibacillus polymyxa ZR-4, the paenibacillus polymyxa ZR-4 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is May 17, 2024, and the preservation number is CGMCC No.30589. The paenibacillus polymyxa ZR-4 is named as Paenibacillus polymyxa ZR-4. The paenibacillus polymyxa ZR-4 provided by the invention not only can promote the growth of plants such as strawberries, corns and rice, but also has a biological control effect on agricultural diseases such as strawberry gray mold and corn southern leaf blight, has an excellent antagonistic effect on pathogenic bacteria of various plant diseases, and has a wide application prospect and development space.

The invention relates to microbacterium arbor H17 and application thereof in preventing and treating plant diseases. The microbacterium arbor is preserved in the China Center for Type Culture Collection, and the preservation number of the microbacterium arbor is CCTCC NO: M 20242129. The microbacterium arbor can effectively inhibit plant pathogenic bacteria Ralstonia solanacearum, can significantly reduce the occurrence of tomato fusarium wilt, can effectively reduce the death rate (reduced from 48% to 4.4%) caused by the fusarium wilt, has biocontrol efficiency of 90%, is harmless to human and livestock, has no pollution to the environment, and can be widely applied to the field of biocontrol. Wide application prospects are realized in the aspect of treating related plant diseases.

The invention discloses a precipitation-supported coated slow-release carbon-based iron fertilizer and a preparation method thereof.The preparation method specifically comprises the steps that a biological crustacean heavy metal adsorbent is soaked in a ferrous sulfate solution and then soaked in a calcium hydroxide solution again after low-temperature drying, finally, dried particles are granulated and subjected to coating treatment, and the coated slow-release carbon-based iron fertilizer is obtained. The coated slow-release iron fertilizer is loaded in a precipitation mode, a mixture of precipitated ferric hydroxide and ferrous hydroxide is uniformly dispersed and attached to the interiors of pore channels of the porous adsorbent, and the precipitated ferric hydroxide and the ferrous hydroxide are wrapped by a byproduct calcium sulfate in the porous adsorbent, so that long-acting slow release of iron ions can be realized; the rice cadmium arsenic antagonistic iron fertilizer has the advantages that the rice cadmium arsenic antagonistic effect can be achieved, the oxygen content of soil can be reduced, fine quantitative use of the coated iron fertilizer in the seedling raising period is matched, iron ion antagonism and a precipitation type adsorption load mode are carried out in the whole rice growth process, the iron ion release period is greatly prolonged, and the iron fertilizer has the excellent rice cadmium arsenic antagonism effect and has the excellent application prospect.

The invention discloses a strain of Pseudomonas sp., which is named as Pseudomonas sp. CY58, is preserved in China Center for Culture Collection on September 27, 2024, and has a preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20242104. The invention also discloses a method for preparing the Pseudomonas sp. The invention also discloses a microbial agent containing the Pseudomonas sp. CY58 as well as a preparation method and application of the microbial agent. The microbial agent containing the Pseudomonas sp. CY58 or the fermentation broth thereof disclosed by the invention can effectively prevent and treat tea anthracnose, pepper anthracnose, apple anthracnose and sorghum anthracnose, obviously slows down the plant disease condition, has a good prevention and treatment effect, especially has the best prevention and treatment effect on pepper anthracnose, and has a good application prospect. A new direction is found for preventing and treating the plant anthracnose.

The invention relates to the technical field of microorganisms, and discloses a strain of Paraburkholderia phytofirmware Ccw389 used for growth promotion of coptis chinensis, biological prevention and control of coptis chinensis planting diseases and soil improvement of coptis chinensis planting, and the strain of the Paraburkholderia phytofirmware Ccw389 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 3, 2024, and the preservation number is CGMCC No.31836. The invention further discloses a preparation method of the strain of the Paraburkholderia phytofirmware Ccw389. The Burkholderia paraburica Ccw389 provided by the invention can be used for preventing and treating the root rot of coptis chinensis, is beneficial to reducing the application amount of chemical pesticides in the planting process of coptis chinensis, and has the characteristics of no toxicity, no harm and the like; meanwhile, the strain can promote the healthy growth of coptis chinensis and also has a certain effect on improvement of coptis chinensis planting soil through the processes of dissolving phosphorus, dissolving potassium, fixing nitrogen and the like as well as the modes of generating siderophores, ACC deaminase and the like.

The invention discloses a penicillium fungus GS218, a penicillium fungus GS218 conidium suspension and a preparation method and application of the penicillium fungus GS218 conidium suspension. The penicillium fungus GS218 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC M 20241477, and the penicillium fungus GS218 is separated from fruits of trifoliate orange in a Hunan Institute of Gardening Institute of Hunan Province in the East Lake Street, Hibiscus District, Changsha City, Hunan Province. The penicillium fungus has a relatively good antagonistic effect on HLB13 fusarium buchneri, colletotrichum spinosus, fusarium solani, fusarium oxysporum cucumber specialized type and botrytis cinerea; the composition can be used for effectively preventing and treating carrot diseases caused by HLB13 fusarium brucellosis, pepper anthracnose caused by colletotrichum spinosus, pepper root rot caused by fusarium solani, cucumber fusarium wilt caused by fusarium oxysporum cucumber specialization and botrytis cinerea caused by botrytis cinerea, and has remarkable and stable technical effects; the method has wide application value in the technical field of microbial strain application.

The present invention discloses Streptomyces SC-3 and its composition and use. The Streptomyces SC-3 strain is deposited in China Center for Type Culture Collection with the deposit number CCTCC NO:M 2024906. The present invention finds that the Streptomyces SC-3 strain itself has high efficiency and broad-spectrum antibacterial activity, and finds that the Streptomyces SC-3 strain has a significant antagonistic effect on kiwifruit soft rot bacteria. It is verified by indoor experiments that the speed of kiwifruit fruit decay caused by Phomopsis can be slowed down. Its fermentation liquid has good temperature stability, and a variety of antibacterial substances are identified from its sterile fermentation liquid, including antibiotics such as antimycin, tetramycin, candidacidin, streptomycin, etc., which are widely used in biological control. The speed of fruit decay caused by the infection of Botrytis cinerea after inoculation can be effectively inhibited.

The present invention relates to the technical field of biocontrol bacteria, and in particular to the application of Paenibacillus polymyxa ZJU11 in preventing and controlling mango anthracnose. The present invention provides the application of Paenibacillus polymyxa ZJU11 in preventing and controlling mango anthracnose. The taxonomic nomenclature of the Paenibacillus polymyxa ZJU11 is Paenibacillus polymyxa ZJU11, with a deposit number of CCTCC NO: M 20241847. The polymyxa bacillus ZJU11 of the present invention is a biocontrol strain isolated from the root system of watermelon and has good antagonistic effect on mango anthracnose. The intracellular extract of the strain has good antagonistic effect on the dominant pathogens of mango postharvest anthracnose and inhibits mango anthracnose, and can be used to prevent and control mango postharvest anthracnose and alleviate the symptoms of mango postharvest anthracnose.

The invention provides a Coxsackie cocori qzb219 strain and application thereof, the Coxsackie cocori qzb219 strain is named as Kosakonia cowaniiqzb219 in taxonomy, and the preservation number of the Coxsackie cocori qzb219 strain is CGMCC (China General Microbiological Culture Collection Center) NO.31519. The coxakella cocori has a good disease prevention effect on tobacco bacterial wilt and tobacco fusarium root rot, and can promote the growth of tobacco seedlings. The coxakella cocori qzb219 disclosed by the invention can be used for efficiently inhibiting the growth of ralstonia solanacearum and fusarium root rot of tobacco, has the activity of amylase and protease, and has the effect of promoting the growth of tobacco seedlings. The strain grows fast, is easy to ferment, and has good compatibility with current main bactericides thiediazole copper and zinc thiazole for preventing and controlling ralstonia solanacearum. The strain can effectively prevent and control tobacco bacterial wilt and tobacco fusarium root rot, has a certain growth promoting effect on tobacco seedlings, and is a biocontrol strain with great market potential.

The invention relates to the technical field of microorganisms, in particular to Pseudomonas S11 and application thereof, the Pseudomonas S11 is preserved in China Center for Type Culture Collection, the preservation address is No. 299, eight road, Wuchang District, Wuhan City, Hubei Province, the preservation date is March 21, 2022, and the preservation number is CCTCC NO: M2022294. The pseudomonas S11 provided by the invention not only can promote the growth of plants such as strawberries, corns and rice, but also has a biological control effect on agricultural diseases such as strawberry gray mold and corn southern leaf blight, has an excellent antagonistic effect on pathogenic bacteria of various plant diseases, and has a wide application prospect and development space.

The present invention relates to the technical field of biocontrol bacteria, and in particular to a strain of Gluconobacter freundii AY1 and its application in preventing and controlling mango anthracnose. The present invention provides a strain of Gluconobacter freundii ( Gluconobacter frateurii )AY1, with a deposit number of CCTCC NO: M 20241846. The Gluconobacter freundii AY1 of the present invention is isolated and purified by the inventor from the surface of the "Golden Glory" mango fruit picked from the mango plantation area in Jiyang District, Sanya City, Hainan Province, and is a biocontrol strain with good antagonistic effect on mango postharvest anthracnose (inhibition index is 0.513-0.555). The strain has good antagonistic effect on the dominant pathogens of mango postharvest anthracnose and inhibits mango anthracnose, and can be used to prepare a bacterial agent for preventing and controlling mango postharvest anthracnose, and alleviate the symptoms of mango postharvest anthracnose.

The present invention belongs to the field of microbial technology, and specifically relates to a biocontrol bacterium DHR14 for preventing and treating red root disease of rubber trees and its application. The biocontrol bacterium DHR14 for preventing and treating red root disease of rubber trees of the present invention has a broad-spectrum antibacterial activity, has a good antagonistic effect on a variety of pathogens such as red root disease of rubber trees, and is a potential biological agent for preventing and treating red root disease of rubber trees, rice seedling disease, eggplant wilt, etc., and the biocontrol bacterium for red root disease of rubber trees of the present invention also has good ability to produce protease, protease, cellulase, β-glucanase, amylase, siderophore, etc. The biocontrol bacterium DHR14 for preventing and treating red root disease of rubber trees of the present invention has a broad development space, and provides a new candidate strain for the biological control of pathogens such as red root disease of rubber trees, rice sheath blight, and rice seedling disease.

The invention relates to a Trichoderma asperellum strain and application thereof, the Trichoderma asperellum strain is named as Trichoderma asperellum TA-1, the Trichoderma asperellum strain is preserved in the China Center for Type Culture Collection (CGTCC), and the preservation number is CGTCC NO.M20232562; the Trichoderma asperellum TA-1 disclosed by the invention has an excellent inhibition effect on the tobacco target leaf spot, and particularly, the inhibition effect on tobacco leaves can be up to 60% or above. The Trichoderma asperellum TA-1 disclosed by the invention has the advantages that the Trichoderma asperellum TA-1 has relatively strong activity of antagonizing pathogenic bacteria of tobacco target leaf spot, is lasting and stable in antibacterial effect, has a huge potential of developing a novel, efficient and broad-spectrum biocontrol bactericide, and has a certain growth promoting effect on plants.

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a ginkgo biloba extract, a preparation method and application of the ginkgo biloba extract in improvement of cerebral ischemia. The preparation method of the ginkgo biloba extract comprises the following steps: (1) crushing dried ginkgo biloba, and adding a compound enzyme aqueous solution for soaking to obtain a mixture 1; (2) adding a surfactant and absolute ethyl alcohol into the mixture 1, performing ultrasonic extraction, and filtering to obtain a ginkgo leaf extracting solution; and (3) filtering the ginkgo leaf extracting solution through a ceramic membrane, concentrating until the ginkgo leaf extracting solution has no ethanol smell, adding the ginkgo leaf extracting solution into a polyamide resin and macroporous resin combined resin column for elution, collecting eluent, concentrating and drying to obtain the ginkgo leaf extract. According to the ginkgo biloba extract, the specific compound enzyme, the surfactant and the purified resin are selected, and the ginkgo biloba extract obtained through extraction is high in effective component content and remarkable in cerebral ischemia improving effect.

The present invention provides a strain of Bacillus spizenzae ( Bacillus spizizenii )HMQ20YJ19, whose deposit number is CCTCC M 20231085. The present invention also provides its use in preparing a drug for preventing and treating pathogenic fungi. The Bacillus spizenzae of the present invention is suitable for the soil ecological environment and climatic conditions of Gansu, and can be used for the prevention and control of plateau summer vegetable diseases in Gansu.

The invention discloses a high-yield, non-autolyzed Coprinus comatus and its application. The registration number of the strain in the General Microbiological Center of China Microbiological Culture Collection Administration Committee is CGMCC No. 22447. The strain is obtained by hybridization and selection of Coprinus comatus strains using homokaryotic monospore strain hybridization technology. The strain is a new variety of Coprinus comatus with excellent traits. Compared with other hybrid strains and parent strains, the strain has a high bioconversion rate of 117.5%; a slow autolysis rate, which is slower than other hybrid strains, 2 days slower than the cc900 parent, and 8 days slower than the 935 parent; and a strong ability to resist pathogenic bacteria, and has a strong antagonistic ability to Lecanicillium fungicola, Mucor racemosus, and Trichoderma virens.

The invention relates to a streptomyces X-57, a microecological preparation thereof and a preparation method of the microecological preparation, the streptomyces is preserved in the China Center for Type Culture Collection, the strain preservation number is CCTCC NO: M 20242239, the classification name is streptomyces X-57, and the Latin name is Streptomyces sp.X-57. The invention also discloses a preparation method of the microecological preparation, the streptomyces is preserved in the China Center for Type Culture Collection, and the strain preservation number is CCTCC NO: M 20242239. The microecological preparation is prepared by fermenting streptomycete X-57. The invention also discloses a preparation method of the microecological preparation. A microecological preparation which is prepared from the strain through a corresponding method and is suitable for aquaculture acts on freshwater fishes in a feed additive mode and has broad-spectrum activity of inhibiting fish pathogenic bacteria, and the streptomyces X-57 is used as a feed additive to feed fishes, especially crucian. The growth performance and the immunity of fishes, especially crucian carps, can be improved, and the intestinal flora can be improved. Wherein the effect of resisting infection of aeromonas hydrophila on crucian carps is particularly remarkable, meanwhile, the culture medium can be stably colonized in the crucian carps, the immunocompetence of the crucian carps is effectively improved, and the culture medium has a certain effect of promoting the growth of the crucian carps.

The invention discloses a soil repair agent based on trace elements of coal gangue and a preparation method thereof, which belongs to the technical field of soil repair agents, and comprises the following steps: mixing dark fermentation strains with beneficial soil bacteria to obtain composite strains, coating the composite strains with starch, and then mixing with a bio-based foaming agent to obtain a composite foaming agent; mixing modified cellulose, a composite foaming agent, coal gangue powder and a gelling agent to obtain a hydrogel, and freeze-drying to obtain a loaded coal gangue particle aerogel; mixing the loaded coal gangue particle aerogel with trace elements, adding peat soil, lignite, vermiculite, and corn stalks for stirring, and drying and packaging to obtain a soil repair agent. The starch-encapsulated strains in the composite foaming agent have better stability and can be better propagated and developed in the soil; the encapsulated hydrogen-producing strains can better complete the dark fermentation reaction, increase the biohydrogen content in the soil, and restore soil fertility.

The present invention discloses a strain of Bacillus Velez FS‑3 and the bacteriocin produced and its application, belonging to the field of microbial application technology. The deposit number of the Bacillus Velez FS‑3 is CGMCC NO.30514. The strain has a good antagonistic effect on Gram-negative bacteria such as multidrug-resistant Escherichia coli. The bacteriocin PFS‑3 prepared by fermentation of the strain shows good safety and bactericidal activity, and has a good therapeutic effect on mice infected with Escherichia coli resistant to polymyxins and carbapenems. The bacteriocin PFS‑3 prepared by fermentation of Bacillus Velez FS‑3 of the present invention is expected to enrich the current existing antimicrobial drug library and provide new hope for the treatment of multidrug-resistant Gram-negative bacteria infection.

The invention relates to pseudomonas wrinkularis capable of resisting botrytis cinerea, leaf mould and early blight bacteria of tomatoes and application of the pseudomonas wrinkularis. The pseudomonas wrinkularis is pseudomonas wrinkularis YC-2305, and the preservation number of the pseudomonas wrinkularis YC-2305 is CGMCC (China General Microbiological Culture Collection Center) NO.29940; the thalli and the fermentation liquor of the Pseudomonas wrinkularis YC-2305 disclosed by the invention have relatively strong antibacterial activity on various fungal diseases of tomatoes, and the antibacterial effect is lasting and stable, so that the Pseudomonas wrinkularis YC-2305 has a huge potential of developing a novel, efficient and broad-spectrum biocontrol bactericide from the Pseudomonas wrinkularis YC-2305.

The invention provides a method for promoting growth of anoectochilus formosanus seedlings. According to the method for promoting the growth of the anoectochilus formosanus seedlings, the anoectochilus formosanus seedlings are sprayed with the mycorrhizal fungi culture solution of the orange-yellow magnolia denudate, so that stems and leaves of the anoectochilus formosanus seedlings are infiltrated with the mycorrhizal fungi culture solution. The method for promoting the growth of the anoectochilus formosanus seedlings can obviously antagonize the stem rot pathogenic bacteria of the anoectochilus formosanus seedlings.

The application discloses a bacillus urosemiae and a bacteriostatic preparation based on the bacillus urosemiae and application thereof, and belongs to the technical field of microbial application. Paenibacillus urinalis The bacillus urosemiae is in Latin and is preserved in the China Center for Type Culture Collection, Wuhan University, Wuhan, China, has a preservation number of CCTCC NO: M 20242902, and was preserved on December 25, 2024, and is named Y6N9. The bacillus urosemiae can be applied to preparation of a preparation for antagonizing pathogenic bacteria in aquaculture, and has important application value in disease prevention and control of Pelteobagrus fulvidraco.

The present invention belongs to the technical field of biological control, and specifically relates to a method for abnormal Wickham yeast to control brown rot, aspergillus disease, soft rot and storage and preservation of peach fruits after harvest. The steps are as follows: firstly, a 1×10 8 cells / mL of yeast suspension; select peach fruits, disinfect, rinse and dry them, and then divide them into three groups, drill holes on the surface of the peach fruits and inject the yeast suspension, and after a period of time, inject spore suspensions of American and Australian type drupe brown rot fungi, Aspergillus niger and Rhizopus stolonifer in the holes of the three groups of peach fruits in the same volume as the yeast suspension, so as to achieve effective control of peach brown rot, aspergillus disease and soft rot; or directly spray the yeast suspension evenly on the surface of the peach fruits and dry them naturally, so as to achieve the purpose of preventing and controlling natural diseases of peach fruits after harvest and storing and preserving them; the present invention has no adverse and significant effects on the main quality indicators of peach fruits after harvest while effectively controlling the diseases, is easy to operate, safe and environmentally friendly, and has significant economic and social benefits.

The invention provides an alternaria alternata strain I-Y6-1 and an application of the alternaria alternata strain I-Y6-1 in a preparation for resisting vibrio parahaemolyticus. The strain is preserved in China General Microbiological Culture Collection Center on July 24, 2024, and the preservation number is CGMCC NO.41473. The invention further provides a preparation method of the alternaria alternata strain I-Y6-1. The alternaria alternata provided by the invention has a relatively strong antagonistic effect on vibrio parahaemolyticus, has huge application potential on prevention and treatment of diseases caused by vibrio parahaemolyticus, especially prevention and treatment of aquaculture diseases, and provides a thought for biological prevention and treatment of aquatic vibrio diseases.

A strain of Streptomyces XL-34, a microecological preparation thereof and a preparation method thereof, wherein the Streptomyces is deposited in the China Center for Type Culture Collection, the strain deposit number is CCTCC NO: M 20231087, the classification name is: novel Streptomyces XL-34, the Latin name is: Streptomyces sp. XL‑34. The microecological preparation is made by fermentation of Streptomyces XL‑34. The present invention also includes a method for preparing the microecological preparation. The microecological preparation suitable for aquaculture prepared by the strain through a corresponding method acts on freshwater fish in the form of a feed additive, has a broad spectrum of activity in inhibiting fish pathogens, and its protection rate reaches 41.67%, among which the effect on freshwater fish against infection by Aeromonas veronii is particularly significant. At the same time, it can also stably colonize in freshwater fish and effectively improve the immunity of freshwater fish, and has a certain effect on promoting fish growth.

This invention proposes a new class of compounds that effectively antagonize integrin α4β7, which are compounds represented by formula (Y), or stereoisomers, tautomers, pharmaceutically acceptable salts, or prodrugs of compounds represented by formula (Y):

The present invention relates to the technical field of microbial agents, and discloses a biochar agent based on Bacillus atrophaeus A126, and a preparation method and application thereof. The preparation method comprises the steps of: inoculating Bacillus atrophaeus A126 into a BU culture medium and culturing for 72 to 120 hours to obtain a BU fermentation product; mixing the BU fermentation product and nutrients with pine biochar to obtain a first product; mixing the first product with a mixed powder of a suspension of Bacillus atrophaeus A126 and pine biochar to obtain a second product; mixing the second product with a mixed solution of the BU fermentation product and sodium alginate to obtain a third product; adding the third product to a calcium chloride solution to obtain a biochar agent; wherein the deposit number of Bacillus atrophaeus A126 is CGMCC NO.32444. The biochar agent prepared by the present invention has good and stable antibacterial effect, and can significantly reduce the incidence and disease index of potato scab after use.

This invention provides a strain of *Pseudomonas aeruginosa* RL76, its bacterial suspension, its preparation method, and its applications, belonging to the field of agricultural microbiology technology. The *Pseudomonas aeruginosa* RL76 was deposited at the China General Microbiological Culture Collection Center (CGMCC) on January 26, 2026, with accession number CGMCC No. 37531. This invention utilizes a mixture of filter mud and soil for sugar beet cultivation. It was found that, under the same pathogen inoculation conditions, the filter mud treatment significantly alleviated the occurrence of damping-off disease in sugar beets compared to the non-filter mud treatment, suggesting that the filter mud plays a role in increasing the relative abundance of beneficial microorganisms in the soil. Therefore, this invention isolated a strain of *Pseudomonas aeruginosa* RL76 with highly efficient antagonistic activity against *Rhizoctonia solani* from the rhizosphere soil of sugar beets cultivated using a mixture of filter mud and soil, and for the first time discovered its effective control of sugar beet damping-off disease while also promoting sugar beet growth, representing a groundbreaking achievement.