31results about How to "Strong antagonistic effect" patented technology

The invention discloses a strain of Pseudomonas sp., which is named as Pseudomonas sp. CY58, is preserved in China Center for Culture Collection on September 27, 2024, and has a preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20242104. The invention also discloses a method for preparing the Pseudomonas sp. The invention also discloses a microbial agent containing the Pseudomonas sp. CY58 as well as a preparation method and application of the microbial agent. The microbial agent containing the Pseudomonas sp. CY58 or the fermentation broth thereof disclosed by the invention can effectively prevent and treat tea anthracnose, pepper anthracnose, apple anthracnose and sorghum anthracnose, obviously slows down the plant disease condition, has a good prevention and treatment effect, especially has the best prevention and treatment effect on pepper anthracnose, and has a good application prospect. A new direction is found for preventing and treating the plant anthracnose.

The invention discloses a penicillium fungus GS218, a penicillium fungus GS218 conidium suspension and a preparation method and application of the penicillium fungus GS218 conidium suspension. The penicillium fungus GS218 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC M 20241477, and the penicillium fungus GS218 is separated from fruits of trifoliate orange in a Hunan Institute of Gardening Institute of Hunan Province in the East Lake Street, Hibiscus District, Changsha City, Hunan Province. The penicillium fungus has a relatively good antagonistic effect on HLB13 fusarium buchneri, colletotrichum spinosus, fusarium solani, fusarium oxysporum cucumber specialized type and botrytis cinerea; the composition can be used for effectively preventing and treating carrot diseases caused by HLB13 fusarium brucellosis, pepper anthracnose caused by colletotrichum spinosus, pepper root rot caused by fusarium solani, cucumber fusarium wilt caused by fusarium oxysporum cucumber specialization and botrytis cinerea caused by botrytis cinerea, and has remarkable and stable technical effects; the method has wide application value in the technical field of microbial strain application.

The present invention relates to the technical field of biocontrol bacteria, and in particular to the application of Paenibacillus polymyxa ZJU11 in preventing and controlling mango anthracnose. The present invention provides the application of Paenibacillus polymyxa ZJU11 in preventing and controlling mango anthracnose. The taxonomic nomenclature of the Paenibacillus polymyxa ZJU11 is Paenibacillus polymyxa ZJU11, with a deposit number of CCTCC NO: M 20241847. The polymyxa bacillus ZJU11 of the present invention is a biocontrol strain isolated from the root system of watermelon and has good antagonistic effect on mango anthracnose. The intracellular extract of the strain has good antagonistic effect on the dominant pathogens of mango postharvest anthracnose and inhibits mango anthracnose, and can be used to prevent and control mango postharvest anthracnose and alleviate the symptoms of mango postharvest anthracnose.

The present invention relates to the technical field of biocontrol bacteria, and in particular to a strain of Gluconobacter freundii AY1 and its application in preventing and controlling mango anthracnose. The present invention provides a strain of Gluconobacter freundii ( Gluconobacter frateurii )AY1, with a deposit number of CCTCC NO: M 20241846. The Gluconobacter freundii AY1 of the present invention is isolated and purified by the inventor from the surface of the "Golden Glory" mango fruit picked from the mango plantation area in Jiyang District, Sanya City, Hainan Province, and is a biocontrol strain with good antagonistic effect on mango postharvest anthracnose (inhibition index is 0.513-0.555). The strain has good antagonistic effect on the dominant pathogens of mango postharvest anthracnose and inhibits mango anthracnose, and can be used to prepare a bacterial agent for preventing and controlling mango postharvest anthracnose, and alleviate the symptoms of mango postharvest anthracnose.

The present invention belongs to the field of microbial technology, and specifically relates to a biocontrol bacterium DHR14 for preventing and treating red root disease of rubber trees and its application. The biocontrol bacterium DHR14 for preventing and treating red root disease of rubber trees of the present invention has a broad-spectrum antibacterial activity, has a good antagonistic effect on a variety of pathogens such as red root disease of rubber trees, and is a potential biological agent for preventing and treating red root disease of rubber trees, rice seedling disease, eggplant wilt, etc., and the biocontrol bacterium for red root disease of rubber trees of the present invention also has good ability to produce protease, protease, cellulase, β-glucanase, amylase, siderophore, etc. The biocontrol bacterium DHR14 for preventing and treating red root disease of rubber trees of the present invention has a broad development space, and provides a new candidate strain for the biological control of pathogens such as red root disease of rubber trees, rice sheath blight, and rice seedling disease.

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a ginkgo biloba extract, a preparation method and application of the ginkgo biloba extract in improvement of cerebral ischemia. The preparation method of the ginkgo biloba extract comprises the following steps: (1) crushing dried ginkgo biloba, and adding a compound enzyme aqueous solution for soaking to obtain a mixture 1; (2) adding a surfactant and absolute ethyl alcohol into the mixture 1, performing ultrasonic extraction, and filtering to obtain a ginkgo leaf extracting solution; and (3) filtering the ginkgo leaf extracting solution through a ceramic membrane, concentrating until the ginkgo leaf extracting solution has no ethanol smell, adding the ginkgo leaf extracting solution into a polyamide resin and macroporous resin combined resin column for elution, collecting eluent, concentrating and drying to obtain the ginkgo leaf extract. According to the ginkgo biloba extract, the specific compound enzyme, the surfactant and the purified resin are selected, and the ginkgo biloba extract obtained through extraction is high in effective component content and remarkable in cerebral ischemia improving effect.

The present invention provides a strain of Bacillus spizenzae ( Bacillus spizizenii )HMQ20YJ19, whose deposit number is CCTCC M 20231085. The present invention also provides its use in preparing a drug for preventing and treating pathogenic fungi. The Bacillus spizenzae of the present invention is suitable for the soil ecological environment and climatic conditions of Gansu, and can be used for the prevention and control of plateau summer vegetable diseases in Gansu.

The invention discloses a high-yield, non-autolyzed Coprinus comatus and its application. The registration number of the strain in the General Microbiological Center of China Microbiological Culture Collection Administration Committee is CGMCC No. 22447. The strain is obtained by hybridization and selection of Coprinus comatus strains using homokaryotic monospore strain hybridization technology. The strain is a new variety of Coprinus comatus with excellent traits. Compared with other hybrid strains and parent strains, the strain has a high bioconversion rate of 117.5%; a slow autolysis rate, which is slower than other hybrid strains, 2 days slower than the cc900 parent, and 8 days slower than the 935 parent; and a strong ability to resist pathogenic bacteria, and has a strong antagonistic ability to Lecanicillium fungicola, Mucor racemosus, and Trichoderma virens.

The invention relates to a streptomyces X-57, a microecological preparation thereof and a preparation method of the microecological preparation, the streptomyces is preserved in the China Center for Type Culture Collection, the strain preservation number is CCTCC NO: M 20242239, the classification name is streptomyces X-57, and the Latin name is Streptomyces sp.X-57. The invention also discloses a preparation method of the microecological preparation, the streptomyces is preserved in the China Center for Type Culture Collection, and the strain preservation number is CCTCC NO: M 20242239. The microecological preparation is prepared by fermenting streptomycete X-57. The invention also discloses a preparation method of the microecological preparation. A microecological preparation which is prepared from the strain through a corresponding method and is suitable for aquaculture acts on freshwater fishes in a feed additive mode and has broad-spectrum activity of inhibiting fish pathogenic bacteria, and the streptomyces X-57 is used as a feed additive to feed fishes, especially crucian. The growth performance and the immunity of fishes, especially crucian carps, can be improved, and the intestinal flora can be improved. Wherein the effect of resisting infection of aeromonas hydrophila on crucian carps is particularly remarkable, meanwhile, the culture medium can be stably colonized in the crucian carps, the immunocompetence of the crucian carps is effectively improved, and the culture medium has a certain effect of promoting the growth of the crucian carps.

The present invention discloses a strain of Bacillus Velez FS‑3 and the bacteriocin produced and its application, belonging to the field of microbial application technology. The deposit number of the Bacillus Velez FS‑3 is CGMCC NO.30514. The strain has a good antagonistic effect on Gram-negative bacteria such as multidrug-resistant Escherichia coli. The bacteriocin PFS‑3 prepared by fermentation of the strain shows good safety and bactericidal activity, and has a good therapeutic effect on mice infected with Escherichia coli resistant to polymyxins and carbapenems. The bacteriocin PFS‑3 prepared by fermentation of Bacillus Velez FS‑3 of the present invention is expected to enrich the current existing antimicrobial drug library and provide new hope for the treatment of multidrug-resistant Gram-negative bacteria infection.

The invention provides a method for promoting growth of anoectochilus formosanus seedlings. According to the method for promoting the growth of the anoectochilus formosanus seedlings, the anoectochilus formosanus seedlings are sprayed with the mycorrhizal fungi culture solution of the orange-yellow magnolia denudate, so that stems and leaves of the anoectochilus formosanus seedlings are infiltrated with the mycorrhizal fungi culture solution. The method for promoting the growth of the anoectochilus formosanus seedlings can obviously antagonize the stem rot pathogenic bacteria of the anoectochilus formosanus seedlings.

The application discloses a bacillus urosemiae and a bacteriostatic preparation based on the bacillus urosemiae and application thereof, and belongs to the technical field of microbial application. Paenibacillus urinalis The bacillus urosemiae is in Latin and is preserved in the China Center for Type Culture Collection, Wuhan University, Wuhan, China, has a preservation number of CCTCC NO: M 20242902, and was preserved on December 25, 2024, and is named Y6N9. The bacillus urosemiae can be applied to preparation of a preparation for antagonizing pathogenic bacteria in aquaculture, and has important application value in disease prevention and control of Pelteobagrus fulvidraco.

The invention provides an alternaria alternata strain I-Y6-1 and an application of the alternaria alternata strain I-Y6-1 in a preparation for resisting vibrio parahaemolyticus. The strain is preserved in China General Microbiological Culture Collection Center on July 24, 2024, and the preservation number is CGMCC NO.41473. The invention further provides a preparation method of the alternaria alternata strain I-Y6-1. The alternaria alternata provided by the invention has a relatively strong antagonistic effect on vibrio parahaemolyticus, has huge application potential on prevention and treatment of diseases caused by vibrio parahaemolyticus, especially prevention and treatment of aquaculture diseases, and provides a thought for biological prevention and treatment of aquatic vibrio diseases.

This invention proposes a new class of compounds that effectively antagonize integrin α4β7, which are compounds represented by formula (Y), or stereoisomers, tautomers, pharmaceutically acceptable salts, or prodrugs of compounds represented by formula (Y):

The present invention relates to the technical field of microbial agents, and discloses a biochar agent based on Bacillus atrophaeus A126, and a preparation method and application thereof. The preparation method comprises the steps of: inoculating Bacillus atrophaeus A126 into a BU culture medium and culturing for 72 to 120 hours to obtain a BU fermentation product; mixing the BU fermentation product and nutrients with pine biochar to obtain a first product; mixing the first product with a mixed powder of a suspension of Bacillus atrophaeus A126 and pine biochar to obtain a second product; mixing the second product with a mixed solution of the BU fermentation product and sodium alginate to obtain a third product; adding the third product to a calcium chloride solution to obtain a biochar agent; wherein the deposit number of Bacillus atrophaeus A126 is CGMCC NO.32444. The biochar agent prepared by the present invention has good and stable antibacterial effect, and can significantly reduce the incidence and disease index of potato scab after use.

This invention provides a strain of *Pseudomonas aeruginosa* RL76, its bacterial suspension, its preparation method, and its applications, belonging to the field of agricultural microbiology technology. The *Pseudomonas aeruginosa* RL76 was deposited at the China General Microbiological Culture Collection Center (CGMCC) on January 26, 2026, with accession number CGMCC No. 37531. This invention utilizes a mixture of filter mud and soil for sugar beet cultivation. It was found that, under the same pathogen inoculation conditions, the filter mud treatment significantly alleviated the occurrence of damping-off disease in sugar beets compared to the non-filter mud treatment, suggesting that the filter mud plays a role in increasing the relative abundance of beneficial microorganisms in the soil. Therefore, this invention isolated a strain of *Pseudomonas aeruginosa* RL76 with highly efficient antagonistic activity against *Rhizoctonia solani* from the rhizosphere soil of sugar beets cultivated using a mixture of filter mud and soil, and for the first time discovered its effective control of sugar beet damping-off disease while also promoting sugar beet growth, representing a groundbreaking achievement.

The invention belongs to the technical field of agricultural microorganisms, and relates to a Trichoderma koningii strain, a strain fermentation broth thereof and an application of the Trichoderma koningii strain in prevention and treatment of pomegranate fusarium wilt. The invention provides a Trichoderma koningii strain, which is classified and named as Trichoderma koningii, is preserved in Guangdong Microbial Culture Collection Center, and has a preservation number of GDMCC NO: 67115. The trichoderma koningii and the strain fermentation liquor thereof provided by the invention have good bacteriostatic ability, can significantly inhibit growth of sweet potato coracocystis spp. Causing pomegranate fusarium wilt, have the inhibition rate of 88.3%, have the advantages of strong bacteriostatic ability, good control effect and high biological safety, and can be widely applied to prevention and treatment of pomegranate fusarium wilt. The bacillus subtilis has a wide application prospect in the aspect of preventing and treating plant diseases caused by the sweet potato coracocystis longirostris.

The invention relates to the technical field of biocontrol bacteria, in particular to application of Gliocladium mucilaginosum RS44 and a composite flora of the Gliocladium mucilaginosum RS44 and Rahnella RS86 in prevention and treatment of corn root rot. The Gliocladium mucilaginosum RS44 provided by the invention is separated from corn roots which are not subjected to root rot in white cities of Jilin Province, has the characteristics of siderophore production and nitrogen fixation, has a better antagonistic effect on pathogenic bacteria fusarium graminearum, has an inhibition rate of 26.39%, has a good prevention and treatment effect on corn root rot, and can promote corn growth. Besides, the composite flora composed of gliocladium mucilaginosum and rahnella RS86 provided by the invention can effectively inhibit the growth of fusarium graminearum, has a good control effect on the corn root rot, reduces the disease index by 26.32%, and can promote the growth of corn.

The invention belongs to the technical field of biology, and particularly relates to a bacillus amyloliquefaciens strain sf28 and application thereof. The bacillus amyloliquefaciens strain sf28 is separated from rhizosphere soil of a peanut southern blight diseased plant, and the bacillus amyloliquefaciens strain sf28 has the effects of resisting fungi and bacteria and can effectively prevent and treat various plant diseases; comprise peanut bacterial wilt, peanut southern blight, peanut fruit rot, peanut root rot, peanut crown rot, ligustrum lucidum canker and tobacco root rot, and the potting test control effect on the peanut southern blight and the peanut bacterial wilt is remarkable.

The present invention relates to the technical field of agricultural fertilizers, and proposes a microbial fertilizer and a preparation method thereof, comprising the following components by weight: 21-30 parts of composite microbial powder, 20-30 parts of straw carbon, 20-30 parts of soybean meal, and 10-20 parts of chicken manure; the composite microbial powder is composed of Brevibacillus laterosporus powder, Bacillus megaterium powder, and Bacillus velez powder. Through the above technical scheme, the problem of reduced crop yield and poor quality caused by the large-scale application of pesticides and fertilizers in the crop planting process in the prior art is solved.

This application relates to the field of microbial technology, disclosing a Pseudomonas palleroniana bacterium and its applications, including strain AQY14, whose taxonomic name is Pseudomonas palleroniana, strain number 16S rRNA, and which was deposited at the Guangdong Provincial Microbial Culture Collection Center on January 23, 2026, with accession number GDMCC No:67636. Furthermore, this invention also provides a bacterial agent containing live bacteria of the aforementioned strain AQY14 and its culture. The bacterial culture includes fermentation broth, fermentation filtrate, bacterial suspension, and purified bacterial solution obtained by culturing strain AQY14 in a culture medium. The bacterial agent is a liquid preparation. The culture medium includes Ashby medium, R2A medium, and PDA medium. The provided strain AQY14 possesses multiple growth-promoting and biocontrol functions, exhibiting significant synergistic applications. This strain simultaneously possesses multiple plant growth-promoting characteristics such as nitrogen fixation, dissolution of organic and inorganic phosphorus, potassium solubilization, siderophore production, and IAA production.

The invention relates to the technical field of agricultural microorganisms, and discloses a plant rhizosphere bacillus for antagonizing plant pathogen pectobacterium carotovorum and application, the plant rhizosphere bacillus is bacillus velezensis Mp4-Ha31 and is preserved in the China Center for Type Culture Collection on September 30, 2025, and the preservation number is CCTCC NO: M 20252184. The invention further discloses a preparation method of the plant rhizosphere bacillus for antagonizing the plant pathogen pectobacterium carotovorum and application of the plant rhizosphere bacillus for antagonizing the plant pathogen pectobacterium carotovorum. The plant bacillus velezensis Mp4-Ha31 provided by the invention has the advantages that the antagonism capability on the bacterial plant pathogen pectobacterium carotovorum is relatively strong, the biological pesticide is developed by taking the bacillus velezensis Mp4-Ha31 as an object, and the potential application prospect is realized.

The invention belongs to the technical field of agricultural microorganisms, and relates to bacillus amyloliquefaciens and application thereof. The preservation number of the bacillus amyloliquefaciens D4 is GDMCC No: 66921, the preservation time is September 5, 2025, and the preservation address is Institute of Microbiology, Guangdong Academy of Sciences, Floor 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The invention provides a novel bacillus amyloliquefaciens, and the strain has a relatively strong antagonistic effect on potato black shank and sweet potato stem rot, and is beneficial to being applied to the aspect of preventing and treating related diseases. The strain D4 is convenient to produce and prepare, low in cost, simple in process and beneficial to large-scale manufacturing and use. The strain D4 as well as a bacterial suspension and a fermentation broth thereof can be used as active ingredients of prevention and treatment medicaments to be applied to actual production on a large scale.

Yohimbine derivatives, their preparation methods, and uses. This invention discloses compounds of Formula I and pharmaceutical compositions comprising compounds of Formula I. The compounds of Formula I of this invention, as adrenaline receptor α2A (ADRA2A) antagonists or partial antagonists, can treat and / or prevent inflammatory bowel disease (IBD), particularly ulcerative colitis (UC), Crohn's disease (CD), or enteritis.

The present invention relates to a kind of rugosa Pseudomonas spp. which is resistant to tomato gray mold, leaf mold and early blight fungus and its application. Pseudomonas corrugata YC-2305, whose deposit number is CGMCC NO.29940; Pseudomonas rugosa of the present invention Pseudomonas corrugata Both the bacteria and fermentation liquid of YC‑2305 have strong antibacterial activity against a variety of tomato fungal diseases, and the antibacterial effect is long-lasting and stable, which has great potential for developing new, highly effective and broad-spectrum biocontrol fungicides.

The bacillus subtilis strain against aeromonas and its application, the bacillus subtilis strain of the present application is named bacillus subtilis (wgb0621), and the preservation number is CCTCC NO: M 2026135. Bacillus subtilis The bacillus subtilis strain has good contact inhibition activity on aeromonas veronii and aeromonas hydrophila, can improve the survival rate of grass carp after being infected with aeromonas, has good safety, and is not dependent on diffusible bacteriostatic substances. The bacillus subtilis strain is suitable for biological prevention and control of aeromonas disease in aquaculture, and has good application prospect.

The invention belongs to the technical field of microorganisms, and discloses an NTase protein, a screening method thereof and application of the NTase protein in preparation of an anti-phage signal system antagonist and / or an antibacterial preparation. The NTase protein provided by the invention specifically comprises an amino acid fragment with a sequence as shown in SEQ ID NO: 1 or a variant fragment with at least 70% sequence identity with the sequence as shown in SEQ ID NO: 1, the NTase protein has an excellent antagonistic effect on a CBASS system of prokaryotes, and the infection efficiency of viruses on prokaryotic cells can be improved by inhibiting the CBASS system; therefore, a better antibacterial effect is achieved, and the compound has a very excellent application prospect in research and development of novel antibacterial drugs.

This invention belongs to the field of microbial and biological control technology, specifically relating to a strain of *Bacillus polymyxa* and its application in biocontrol and growth promotion. Specifically, this invention screens and obtains a strain of *Bacillus polymyxa* (… Paenibacillus polymyxa GLZ219, as demonstrated in experiments, exhibits a high inhibition rate against the pathogen causing tomato neck and root rot; simultaneously, this strain also... Rhizoctonia solani It exhibits good antagonistic effects against nine common plant pathogens and can be widely applied to the green control of various crop diseases. Furthermore, *Bacillus polymyxa* GLZ219 possesses nitrogen-fixing and potassium-solubilizing abilities, and can secrete indoleacetic acid and siderophores, effectively promoting tomato plant growth. Tomato seedlings treated with its fermentation broth show a significant increase in plant height. It can control diseases, improve crop growth, and increase crop yield and quality, demonstrating broad application prospects.

The application discloses a Pseudomonas aeruginosa, which is Pseudomonas aeruginosa EB-Q03-02, and the preservation number is CGMCC No.30779; and the application of the Pseudomonas aeruginosa in preventing and treating Graymold, Pythium aphanidermatum and tobacco blight. The Pseudomonas aeruginosa EB-Q03-02 is separated from the root system of healthy passion fruit, has antagonistic effect on the passion fruit blight, and has obvious antagonistic effect on the passion fruit blight caused by tobacco blight. Further, the Pseudomonas aeruginosa EB-Q03-02 can effectively inhibit the Graymold, Pythium aphanidermatum and tobacco blight, and the inhibition rate is as high as 81% or more.

The present invention discloses sulfonamides, methods for preparation thereof and uses thereof, belonging to the technical field of medicinal chemistry. The structure of the sulfonamides of the present invention is shown in Formula I. The present invention also provides use of the compound of Formula I or a salt, solvate, isomer, metabolite, nitrogen-oxide, or prodrug thereof in the manufacture of a medicament for the treatment or prevention of P2X3 and / or P2X2 / 3 receptor-associated diseases. The sulfonamides of the present invention have a potent cough suppressing effect and a significantly prolonged action. Their inhibitory activity against P2X3 is superior to that of Comparative compound 1 and the positive control Gefapixant.