20 results about "Trifoliate orange" patented technology

The invention belongs to the field of plant genetic engineering, and discloses application of a PtNF-YB9 gene of trifoliate orange in drought-resistant genetic improvement of plants. The PtNF-YB9 gene is a transcription factor gene which is separated and cloned from Poncirus trifoliata, and the sequence of the PtNF-YB9 gene is as shown in SEQ ID NO. 1. The gene or the homologous gene thereof is knocked out or inhibited in a plant, the drought resistance of the plant is remarkably reduced, and the drought resistance of the plant is remarkably enhanced by overexpressing the gene or the homologous gene thereof. The development and utilization of the genetic resource are beneficial to reducing the agricultural production cost and realizing the green and environment-friendly agricultural goal.

The anti-blocking vibrating screen structure of the immature bitter orange screening machine comprises a screening basket, a set of baffles are arranged below the screening basket, ejector rods are installed on the upper surfaces of the baffles and correspond to meshes of the screening basket, the ejector rods are connected with a set of limiting plates in a sliding mode, and the limiting plates can be kept flush with the top ends of the ejector rods through elastic limiting assemblies. When the mesh holes do not need to be dredged, the elastic limiting assembly pushes the limiting plate to move upwards, so that the upper surface of the limiting plate is completely flush with the top ends of the ejector rods, screened small impurities such as fiber chippings and grease particles preferentially fall to the surface of the limiting plate instead of directly falling into gaps between the ejector rods, and therefore the mesh holes can be dredged conveniently. The supporting piece is pulled to ascend, the elastic limiting assembly, the baffle, the ejector rod and the limiting plate can be driven to ascend, the limiting plate can make contact with the bottom end of the screen basket firstly, then pulling continues, the ejector rod can penetrate through the limiting plate to be inserted along the meshes of the screen basket, and the blocked meshes are dredged.

The invention discloses a cinnamomum migao bud stock grafting method. The method comprises the steps of seed selection, treatment, stock cultivation, scion collection, grafting, transplanting, seedling stage management and the like. The grafting method adopts a bud seedling stock grafting technology, is an effective way for accelerating the improved variety process of cinnamomum migao, effectively shortens the seedling raising period, is low in seedling raising cost, good in seedling quality, capable of realizing fast growth and early fruiting, simple and convenient to operate, easy to master by the masses, and convenient to popularize and apply.

The invention discloses a citrus two-factor anti-allergy plant extract and a preparation method and application thereof. The citrus two-factor anti-allergy plant extract comprises poncirin and naringin in a mass ratio of (20-25): (6-10). The preparation method of the citrus double-factor anti-allergy plant extract comprises the following steps: carrying out heating reflux on crushed citrus fruits in a 50-80% ethanol solution for 5-8 hours to obtain an extracted crude product; slowly adding an extracted crude product to the column, and controlling the flow rate to be 1-2BV / h; and then eluting with 3-5BV distilled water to remove water-soluble impurities. And eluting with a 30-40% ethanol solution of 2-3BV, collecting the eluent, concentrating, and recrystallizing to obtain the naringin. And eluting with a 50-70% ethanol solution of 2-3BV, collecting the eluent, concentrating, and recrystallizing to obtain poncirin. When the plant extract is used in cosmetics, the cosmetics can have good moisturizing, soothing and other effects.

The invention relates to the technical field of gene engineering, in particular to a trifoliate orange CwPHL7 gene, a cloning method, an expression vector and application. According to the invention, a CwPHL7 gene high-expression arabidopsis thaliana transgenic plant is successfully obtained through cloning of the trifoliate CwPHL7 gene and construction and transformation of an over-expression vector, and drought stress treatment experiments find that the chlorophyll content, malondialdehyde (MDA) content, active oxygen accumulation and other key physiological indexes of the transgenic plant under drought conditions are all significantly superior to those of a non-transgenic plant, so that the CwPHL7 gene has a good application prospect. The Poncirus trifoliate CwPHL7 gene can significantly enhance the drought tolerance of plants, and is a potential drought-resistant breeding gene resource. The invention not only provides new experimental data for plant stress resistance gene function research, but also provides efficient technical means and gene resources for crop drought resistance genetic improvement, and has important theoretical significance and application value.

The invention belongs to the field of plant genetic engineering, and discloses a trifoliate orange transcription factor PtNF-YA3 gene and application thereof in plant drought-resistant genetic improvement. The PtNF-YA3 gene is a transcription factor gene which is separated and cloned from Poncirus trifoliata, and the sequence of the PtNF-YA3 gene is as shown in SEQ ID NO. 1. The gene sequence is utilized to construct an interference vector, and meanwhile, the interference vector is transferred into trifoliate orange by utilizing a virus-induced gene silencing method transformation technology. After transgenic plants are obtained respectively, biological function verification is carried out, and it is shown that the cloned PtNF-YA3 gene has the function of reducing plant drought tolerance. The development and utilization of the genetic resource are beneficial to reducing the agricultural production cost and realizing the green and environment-friendly agricultural goal.

A new and distinct cultivar of lemon plant named ‘CE-D5-1-9-42’, characterized by nearly seedless fruit, early fruiting, and high fruit production. Additionally, the new lemon cultivar also is compatible with trifoliate orange rootstocks, unlike most lemon varieties. This new cultivar has high quality fresh fruit, as well as juice, peel oil, and other lemon by-products viable for processing. Further, given the new lemon cultivar's good HLB tolerance typical of lemons, and its near seedlessness, this new cultivar may have significant dooryard tree potential.

The invention relates to an application of 5HIAA (5-Hydroxyindole-3-acetic acid) in improvement of saline-alkaline resistance of plants. The chemical formula of the 5HIAA (5-Hydroxyindole-3-acetic acid) is C9H9NO3. The invention further relates to a preparation method of the 5HIAA. The compound is a derivative of indoleacetic acid (IAA) and has an additional hydroxyl (-OH) group located at the fifth position of an indole ring. Experimental results show that when 5HIAA is applied to root systems of arabidopsis thaliana, trifoliate orange and soybean, the saline-alkaline resistance of the plants can be improved, the main performance is that the lateral roots of the plants are increased, and the growth of the plants under saline-alkaline stress is promoted. The method has an important application value.

The present invention discloses a trifoliate orange cold-resistant gene CtrBBX32 and its application in genetic improvement of plant cold resistance. The CtrBBX32 gene is a gene of a subfamily of the zinc finger structural protein family isolated and cloned from the extremely cold-resistant citrus material trifoliate orange, and its sequence is shown in SEQ ID No. 1. Interference vectors and overexpression vectors are constructed with this gene, and it is introduced into trifoliate orange through genetic transformation mediated by Agrobacterium tumefaciens and Agrobacterium rhizogenes to obtain transgenic plants. Biological function verification shows that the cloned CtrBBX32 gene of the present invention has the function of regulating plant cold resistance. The discovery of this gene provides a new gene resource for plant stress resistance molecular design and breeding, and provides a new genetic resource for the implementation of green agriculture and water-saving agriculture. The development and utilization of this genetic resource is conducive to reducing agricultural production costs and achieving environmental friendliness.

The invention belongs to the field of plant genetic engineering, and discloses a trifoliate orange transporter CtrNPF2.1 and application thereof in plant salt resistance and root growth genetic improvement, the CtrNPF2.1 gene is a transporter separated and cloned from citrus rootstock trifoliate orange, and is named as CtrNPF2.1, and the sequence of the CtrNPF2.1 gene is shown as SEQ ID NO.1. The invention further discloses a method for preparing the trifoliate orange transporter CtrNPF2.1 gene. An overexpression vector and an interference vector are respectively constructed by the gene, the gene is introduced into trifoliate orange through agrobacterium tumefaciens-mediated genetic transformation, and biological function verification of an obtained transgenic plant shows that the cloned CtrNPF2.1 gene has the functions of controlling the salt resistance of the plant and regulating the growth of a root system. The development and utilization of the genetic resource are beneficial to reducing the agricultural production cost and realizing environmental friendliness.

The utility model relates to the technical field of medical equipment, in particular to a sample processing device for mass spectrometry detection, which comprises an extraction cylinder, the upper end of the extraction cylinder is in threaded connection with a sealing cover, and the front surface of the sealing cover is provided with an internal threaded hole. Through the arrangement of the extraction barrel, a fructus aurantii material needing to be crushed is placed in the grinding seat in the extraction barrel, then the sealing cover is screwed into the upper end of the extraction barrel, when the grinding body enters the grinding seat, the fructus aurantii material can be extruded, and then after quantitative extraction liquid is sucked into the extraction liquid suction rubber head, the fructus aurantii material can be crushed. The method comprises the following steps: driving an external thread connecting sleeve to be screwed into an internal thread hole, then driving a rotating shaft and a grinding body to rotate through a rotating ball head, quickly crushing and grinding a fructus aurantii material, then extruding an extraction liquid suction rubber head to enable quantitative extraction liquid to enter an extraction barrel through a liquid suction pipe, and quickly soaking the ground and crushed fructus aurantii material. The capability of quickly obtaining the standard sample is realized.

The invention discloses nano-state boron as well as a preparation method and application thereof, and belongs to the technical field of fertilizers. The preparation method of the nano-state boron comprises the following steps: S1, respectively dissolving sodium borate and calcium chloride in water to prepare a sodium borate solution and a calcium chloride solution; s2, adding PVP into the calcium chloride solution, and stirring to obtain a mixed solution; and S3, dropwise adding a sodium borate solution into the mixed solution in the step S2 under the stirring action to obtain the nano-state boron. In addition, the invention also provides the nano-state boron prepared by the preparation method or application of the nano-state boron in planting trifoliate orange rootstocks. The nano-state boron provided by the invention can obviously relieve acid stress in plant planting.

This invention belongs to the field of electronic-grade insulating device manufacturing technology, specifically a method for preparing electronic-grade insulating slurry using *Trifoliate orange* (a type of wood remnant). The method uses *Trifoliate orange* pine chips and *Trifoliate orange* fir chips as raw materials, and involves steps such as specific proportion blending, customized cooking, deep washing and purification in a full aqueous phase, oxygen deoxidation, and precise pulping to prepare the electronic-grade insulating slurry. This invention is the first to propose blending *Trifoliate orange* pine chips and fir chips at a dry weight ratio of 4:6 to 6:4, resulting in a synergistic reinforcing effect and significantly improving the tensile strength of the slurry. Simultaneously, deionized water is used for washing and purification throughout the process, strictly controlling the content of metal ions and impurities to ensure the electronic-grade purity of the product. This invention achieves high-value utilization of *Trifoliate orange* resources, and the resulting electronic-grade insulating slurry exhibits excellent performance, meeting and exceeding the GB / T13505-2025 superior grade standard, providing a reliable localized solution for the domestic high-end insulating material market.

The application belongs to the field of plant genetic engineering, and discloses application of a Punica granatum PtNF-YB9 gene in genetic improvement of plant drought resistance. PtNF-YB9 The gene is a transcription factor gene isolated and cloned from Punica granatum, trifoliate orange and has the sequence shown in SEQ ID NO. 1. Knocking out or inhibiting the gene or a homologous gene in plants can significantly reduce drought resistance of the plants, and overexpressing the gene or the homologous gene can significantly enhance drought resistance of the plants. Development and utilization of the genetic resource are conducive to reducing agricultural production cost and achieving the goal of green and environment-friendly agriculture.

The invention discloses a medicine for treating postherpetic neuralgia and a preparation method thereof, and belongs to the technical field of medicines for herpes zoster. Aiming at postherpetic neuralgia, the traditional Chinese medicine composition treats both symptoms and root causes through the synergistic effect of all the components, gypsum is used, and rhizoma coptidis, radix scutellariae, cortex phellodendri and fructus gardeniae are combined, so that the traditional Chinese medicine composition has the effects of strongly clearing heat, purging fire, detoxifying, removing residual damp-heat toxin and relieving causalgia; salvia miltiorrhiza, radix paeoniae rubra, moutan bark and motherwort synergistically promote blood circulation, remove blood stasis, cool blood and improve microcirculation so as to relieve stabbing pain. Scorpio, centipede, lumbricus and other insect drugs go deep into meridians and collaterals to dispel wind and dredge collaterals and strongly relieve Mangnolia officinalis and trifoliate orange have the effects of promoting qi circulation, reducing phlegm and smoothing qi to promote drug efficacy; radix astragali, glossy privet fruit and sliced cornu cervi tonify qi, nourish yin, warm, dredge and repair, and strengthen healthy qi to promote regeneration of nervous tissues; herba schizonepetae and mint dispel wind and permeate superficies, and guide the medicine to directly reach According to the formula, the neuralgia with various properties can be quickly relieved, inflammation can be radically eliminated, circulation can be improved, and nerves can be repaired.

The present invention discloses a Penicillium fungus GS218, a conidia suspension of Penicillium fungus GS218, and a preparation method and application thereof. The Penicillium fungus GS218 has a preservation number of CCTCC NO: M 20241477, and was isolated from the fruit of the trifoliate orange at the base of the Hunan Horticultural Research Institute in Donghu Street, Furong District, Changsha City, Hunan Province. The Penicillium fungus has a good antagonistic effect on HLB13 Fusarium brucei, Colletotrichum spp., Fusarium solani, Fusarium oxysporum cucumber-specific type, and Botrytis cinerea. It can effectively prevent and control carrot diseases caused by HLB13 Fusarium brucei, pepper anthracnose caused by Colletotrichum spp., pepper root rot caused by Fusarium solani, cucumber wilt caused by Fusarium oxysporum cucumber-specific type, and tomato gray mold caused by Botrytis cinerea. It has significant and stable technical effects and has broad application value in the field of microbial strain application technology.

The present invention belongs to the field of plant genetic engineering and discloses a trifoliate orange transporter protein CtrNPF2.1 and its application in genetic improvement of plant salt resistance and root growth. CtrNPF2.1 The gene was derived from the citrus rootstock Poncirus trifoliata ( Citrus trifoliata ) was isolated and cloned and named CtrNPF2.1 , whose sequence is shown in SEQ ID NO.1. The gene was constructed into overexpression and interference vectors respectively, and introduced into trifoliate orange through Agrobacterium-mediated genetic transformation. The obtained transgenic plants were verified by biological function, indicating that the cloned gene of the present invention CtrNPF2.1 The gene has the function of controlling plant salt resistance and regulating root growth. The development and utilization of this genetic resource will help reduce agricultural production costs and achieve environmental friendliness.

The invention provides a method for determining the content of active ingredients in trifoliate orange. The method adopts high performance liquid chromatography (HPLC) to simultaneously determine naringin, trifoliate orange glycoside and imperatorin in the trifoliate orange. The method comprises the following steps: 1) preparing a reference solution and a test solution; 2) HPLC detection: adopting a DimonsilC18 chromatographic column, taking methanol-1% glacial acetic acid as a mobile phase for gradient elution, and controlling the flow rate to be 1.0 mL / min, the column temperature to be 30 DEG C and the detection wavelength to be 283 nm. The method has the beneficial effects that the three active ingredients of naringin, trifoliate glycoside and imperatorin in the trifoliate orange are simultaneously determined, and compared with single-ingredient determination, the quality of the trifoliate orange can be more comprehensively reflected; by optimizing chromatographic conditions and an extraction method, good separation and accurate determination of the three components are ensured, and the method has good specificity and linear relation, high accuracy and good reproducibility, and can provide reliable technical support for quality control, medicinal material screening and resource development of trifoliate orange.

The invention discloses a cultivation method for dwarfing Jinggang honey pomelo crowns, and the specific technical scheme is as follows: collecting trifoliate orange seeds in late October, accelerating germination and sowing in late February of the next year, after 4-6 true leaves grow, selecting robust seedlings with the plant height of about 15cm, transferring the seedlings with soil into a seedling culture nutrition bag, and additionally applying a compound fertilizer; from the middle ten days to the last ten days of September, scions are collected from a Jinggang honey pomelo female parent garden or scion collection seed trees without quarantine diseases, disinfected and subjected to heat preservation and moisture preservation for standby application; when the diameter of the rootstock reaches 0.4 cm or above, grafting is conducted through a bud grafting method, and seedlings which fail to be grafted are screened out within 30-40 days. The crown of the Jinggang honey pomelo can be greatly dwarfed, the management cost can be saved, the fruit yield and quality are improved, and therefore economic benefits are improved.

This invention belongs to the field of plant genetic engineering and discloses the trifoliate orange transcription factor. PtNF-YB15 Genes and their application in the genetic improvement of drought resistance in plants. PtNF-YB15 Genes are derived from trifoliate orange ( Poncirus trifoliata The transcription factor gene isolated and cloned from [the organism] is shown in SEQ ID NO.1. An interference vector was constructed using this gene sequence, and it was then transformed into trifoliate orange using virus-induced gene silencing transformation technology. Biological function verification was performed on the obtained transgenic plants, demonstrating that the transcription factor cloned in this invention [is effective]. PtNF- YB15 The gene has the function of reducing plant drought resistance. The development and utilization of this genetic resource is conducive to reducing agricultural production costs and achieving the goal of green and environmentally friendly agriculture.