13 results about "Trifoliate orange" patented technology

The invention belongs to the field of plant genetic engineering, and discloses application of a PtNF-YB9 gene of trifoliate orange in drought-resistant genetic improvement of plants. The PtNF-YB9 gene is a transcription factor gene which is separated and cloned from Poncirus trifoliata, and the sequence of the PtNF-YB9 gene is as shown in SEQ ID NO. 1. The gene or the homologous gene thereof is knocked out or inhibited in a plant, the drought resistance of the plant is remarkably reduced, and the drought resistance of the plant is remarkably enhanced by overexpressing the gene or the homologous gene thereof. The development and utilization of the genetic resource are beneficial to reducing the agricultural production cost and realizing the green and environment-friendly agricultural goal.

The anti-blocking vibrating screen structure of the immature bitter orange screening machine comprises a screening basket, a set of baffles are arranged below the screening basket, ejector rods are installed on the upper surfaces of the baffles and correspond to meshes of the screening basket, the ejector rods are connected with a set of limiting plates in a sliding mode, and the limiting plates can be kept flush with the top ends of the ejector rods through elastic limiting assemblies. When the mesh holes do not need to be dredged, the elastic limiting assembly pushes the limiting plate to move upwards, so that the upper surface of the limiting plate is completely flush with the top ends of the ejector rods, screened small impurities such as fiber chippings and grease particles preferentially fall to the surface of the limiting plate instead of directly falling into gaps between the ejector rods, and therefore the mesh holes can be dredged conveniently. The supporting piece is pulled to ascend, the elastic limiting assembly, the baffle, the ejector rod and the limiting plate can be driven to ascend, the limiting plate can make contact with the bottom end of the screen basket firstly, then pulling continues, the ejector rod can penetrate through the limiting plate to be inserted along the meshes of the screen basket, and the blocked meshes are dredged.

The invention discloses a cinnamomum migao bud stock grafting method. The method comprises the steps of seed selection, treatment, stock cultivation, scion collection, grafting, transplanting, seedling stage management and the like. The grafting method adopts a bud seedling stock grafting technology, is an effective way for accelerating the improved variety process of cinnamomum migao, effectively shortens the seedling raising period, is low in seedling raising cost, good in seedling quality, capable of realizing fast growth and early fruiting, simple and convenient to operate, easy to master by the masses, and convenient to popularize and apply.

The invention discloses a citrus two-factor anti-allergy plant extract and a preparation method and application thereof. The citrus two-factor anti-allergy plant extract comprises poncirin and naringin in a mass ratio of (20-25): (6-10). The preparation method of the citrus double-factor anti-allergy plant extract comprises the following steps: carrying out heating reflux on crushed citrus fruits in a 50-80% ethanol solution for 5-8 hours to obtain an extracted crude product; slowly adding an extracted crude product to the column, and controlling the flow rate to be 1-2BV / h; and then eluting with 3-5BV distilled water to remove water-soluble impurities. And eluting with a 30-40% ethanol solution of 2-3BV, collecting the eluent, concentrating, and recrystallizing to obtain the naringin. And eluting with a 50-70% ethanol solution of 2-3BV, collecting the eluent, concentrating, and recrystallizing to obtain poncirin. When the plant extract is used in cosmetics, the cosmetics can have good moisturizing, soothing and other effects.

The invention belongs to the field of plant genetic engineering, and discloses a trifoliate orange transcription factor PtNF-YA3 gene and application thereof in plant drought-resistant genetic improvement. The PtNF-YA3 gene is a transcription factor gene which is separated and cloned from Poncirus trifoliata, and the sequence of the PtNF-YA3 gene is as shown in SEQ ID NO. 1. The gene sequence is utilized to construct an interference vector, and meanwhile, the interference vector is transferred into trifoliate orange by utilizing a virus-induced gene silencing method transformation technology. After transgenic plants are obtained respectively, biological function verification is carried out, and it is shown that the cloned PtNF-YA3 gene has the function of reducing plant drought tolerance. The development and utilization of the genetic resource are beneficial to reducing the agricultural production cost and realizing the green and environment-friendly agricultural goal.

The present invention discloses a trifoliate orange cold-resistant gene CtrBBX32 and its application in genetic improvement of plant cold resistance. The CtrBBX32 gene is a gene of a subfamily of the zinc finger structural protein family isolated and cloned from the extremely cold-resistant citrus material trifoliate orange, and its sequence is shown in SEQ ID No. 1. Interference vectors and overexpression vectors are constructed with this gene, and it is introduced into trifoliate orange through genetic transformation mediated by Agrobacterium tumefaciens and Agrobacterium rhizogenes to obtain transgenic plants. Biological function verification shows that the cloned CtrBBX32 gene of the present invention has the function of regulating plant cold resistance. The discovery of this gene provides a new gene resource for plant stress resistance molecular design and breeding, and provides a new genetic resource for the implementation of green agriculture and water-saving agriculture. The development and utilization of this genetic resource is conducive to reducing agricultural production costs and achieving environmental friendliness.

The invention discloses nano-state boron as well as a preparation method and application thereof, and belongs to the technical field of fertilizers. The preparation method of the nano-state boron comprises the following steps: S1, respectively dissolving sodium borate and calcium chloride in water to prepare a sodium borate solution and a calcium chloride solution; s2, adding PVP into the calcium chloride solution, and stirring to obtain a mixed solution; and S3, dropwise adding a sodium borate solution into the mixed solution in the step S2 under the stirring action to obtain the nano-state boron. In addition, the invention also provides the nano-state boron prepared by the preparation method or application of the nano-state boron in planting trifoliate orange rootstocks. The nano-state boron provided by the invention can obviously relieve acid stress in plant planting.

This invention belongs to the field of electronic-grade insulating device manufacturing technology, specifically a method for preparing electronic-grade insulating slurry using *Trifoliate orange* (a type of wood remnant). The method uses *Trifoliate orange* pine chips and *Trifoliate orange* fir chips as raw materials, and involves steps such as specific proportion blending, customized cooking, deep washing and purification in a full aqueous phase, oxygen deoxidation, and precise pulping to prepare the electronic-grade insulating slurry. This invention is the first to propose blending *Trifoliate orange* pine chips and fir chips at a dry weight ratio of 4:6 to 6:4, resulting in a synergistic reinforcing effect and significantly improving the tensile strength of the slurry. Simultaneously, deionized water is used for washing and purification throughout the process, strictly controlling the content of metal ions and impurities to ensure the electronic-grade purity of the product. This invention achieves high-value utilization of *Trifoliate orange* resources, and the resulting electronic-grade insulating slurry exhibits excellent performance, meeting and exceeding the GB / T13505-2025 superior grade standard, providing a reliable localized solution for the domestic high-end insulating material market.

The application belongs to the field of plant genetic engineering, and discloses application of a Punica granatum PtNF-YB9 gene in genetic improvement of plant drought resistance. PtNF-YB9 The gene is a transcription factor gene isolated and cloned from Punica granatum, trifoliate orange and has the sequence shown in SEQ ID NO. 1. Knocking out or inhibiting the gene or a homologous gene in plants can significantly reduce drought resistance of the plants, and overexpressing the gene or the homologous gene can significantly enhance drought resistance of the plants. Development and utilization of the genetic resource are conducive to reducing agricultural production cost and achieving the goal of green and environment-friendly agriculture.

The invention discloses a medicine for treating postherpetic neuralgia and a preparation method thereof, and belongs to the technical field of medicines for herpes zoster. Aiming at postherpetic neuralgia, the traditional Chinese medicine composition treats both symptoms and root causes through the synergistic effect of all the components, gypsum is used, and rhizoma coptidis, radix scutellariae, cortex phellodendri and fructus gardeniae are combined, so that the traditional Chinese medicine composition has the effects of strongly clearing heat, purging fire, detoxifying, removing residual damp-heat toxin and relieving causalgia; salvia miltiorrhiza, radix paeoniae rubra, moutan bark and motherwort synergistically promote blood circulation, remove blood stasis, cool blood and improve microcirculation so as to relieve stabbing pain. Scorpio, centipede, lumbricus and other insect drugs go deep into meridians and collaterals to dispel wind and dredge collaterals and strongly relieve Mangnolia officinalis and trifoliate orange have the effects of promoting qi circulation, reducing phlegm and smoothing qi to promote drug efficacy; radix astragali, glossy privet fruit and sliced cornu cervi tonify qi, nourish yin, warm, dredge and repair, and strengthen healthy qi to promote regeneration of nervous tissues; herba schizonepetae and mint dispel wind and permeate superficies, and guide the medicine to directly reach According to the formula, the neuralgia with various properties can be quickly relieved, inflammation can be radically eliminated, circulation can be improved, and nerves can be repaired.

The present invention belongs to the field of plant genetic engineering and discloses a trifoliate orange transporter protein CtrNPF2.1 and its application in genetic improvement of plant salt resistance and root growth. CtrNPF2.1 The gene was derived from the citrus rootstock Poncirus trifoliata ( Citrus trifoliata ) was isolated and cloned and named CtrNPF2.1 , whose sequence is shown in SEQ ID NO.1. The gene was constructed into overexpression and interference vectors respectively, and introduced into trifoliate orange through Agrobacterium-mediated genetic transformation. The obtained transgenic plants were verified by biological function, indicating that the cloned gene of the present invention CtrNPF2.1 The gene has the function of controlling plant salt resistance and regulating root growth. The development and utilization of this genetic resource will help reduce agricultural production costs and achieve environmental friendliness.

The invention provides a method for determining the content of active ingredients in trifoliate orange. The method adopts high performance liquid chromatography (HPLC) to simultaneously determine naringin, trifoliate orange glycoside and imperatorin in the trifoliate orange. The method comprises the following steps: 1) preparing a reference solution and a test solution; 2) HPLC detection: adopting a DimonsilC18 chromatographic column, taking methanol-1% glacial acetic acid as a mobile phase for gradient elution, and controlling the flow rate to be 1.0 mL / min, the column temperature to be 30 DEG C and the detection wavelength to be 283 nm. The method has the beneficial effects that the three active ingredients of naringin, trifoliate glycoside and imperatorin in the trifoliate orange are simultaneously determined, and compared with single-ingredient determination, the quality of the trifoliate orange can be more comprehensively reflected; by optimizing chromatographic conditions and an extraction method, good separation and accurate determination of the three components are ensured, and the method has good specificity and linear relation, high accuracy and good reproducibility, and can provide reliable technical support for quality control, medicinal material screening and resource development of trifoliate orange.

This invention belongs to the field of plant genetic engineering and discloses the trifoliate orange transcription factor. PtNF-YB15 Genes and their application in the genetic improvement of drought resistance in plants. PtNF-YB15 Genes are derived from trifoliate orange ( Poncirus trifoliata The transcription factor gene isolated and cloned from [the organism] is shown in SEQ ID NO.1. An interference vector was constructed using this gene sequence, and it was then transformed into trifoliate orange using virus-induced gene silencing transformation technology. Biological function verification was performed on the obtained transgenic plants, demonstrating that the transcription factor cloned in this invention [is effective]. PtNF- YB15 The gene has the function of reducing plant drought resistance. The development and utilization of this genetic resource is conducive to reducing agricultural production costs and achieving the goal of green and environmentally friendly agriculture.