Grafting propagation method for seedlings of endangered species trifoliate orange

Abstract

The invention discloses a grafting propagation method for seedlings of endangered species trifoliate orange, and relates to the technical field of grafting propagation of trifoliate orange. Comprising the steps of grafting stock preparation, scion collection and treatment, grafting and management after grafting, and the efficient and stable grafting propagation method for the trifoliate orange is established. According to the grafting method, common trifoliate orange with high adaptability and stress resistance is adopted as the stock for grafting, the grafted trifoliate orange seedlings are high in survival rate and high in disease and pest resistance, and the stress resistance and adaptability of the trifoliate orange are improved. The technical problem that the survival rate of the trifoliate orange is low in the transplanting and returning process is effectively solved. The method has the advantages that the breeding survival rate of the trifoliate orange can be effectively kept, the problem that the fibrous roots of the trifoliate orange are few is solved, the transplanting survival rate is remarkably increased, and the breeding cost is low. The artificial grafting and seedling raising method for the trifoliate orange is established for the first time, and is of great significance to rescue protection of the trifoliate orange and reconstruction of field populations.

Description

Technical Field

[0001] The invention relates to the technical field of plant grafting, and in particular to a grafting propagation method for seedlings of endangered species Fumin trifoliate orange. Background Art

[0002] Fumin trifoliate orange (Poncirus polyandra), also known as wild orange, belongs to the genus Poncirus of the Rutaceae family. It is a new species of the genus Poncirus discovered by Ding Suqin and others at Donggualing on the east side of Laoqing Mountain in Fumin County, Yunnan Province. It is a unique species of the genus Poncirus in Yunnan Province. It has the characteristics of evergreen, strong cold resistance, and is an excellent dwarfing citrus rootstock species. It was listed in the "First Batch of Provincial Key Protected Wild Plants in Yunnan Province" in 1989. Due to the drastic reduction of wild resources of Fumin trifoliate orange, it was included in the "National Key Protected Wild Plants List" (first batch) in 1999. The "Emergency Action Plan for the Rescue and Protection of Extremely Small Population Species in Yunnan Province (2010-2015)" jointly compiled by the Forestry Department and the Science and Technology Department of Yunnan Province has listed Fumin trifoliate orange as one of the extremely small population species that urgently need to be rescued and protected, and its endangered level is rare.

[0003] At present, there is a lack of a grafting propagation method for the seedlings of the endangered species Fumin trifoliate orange with strong disease and insect resistance. Summary of the invention

[0004] The purpose of the invention is to provide a grafting propagation method for seedlings of the endangered species Fumin trifoliate orange with strong disease and insect resistance.

[0005] In order to achieve the above-mentioned object, the present invention adopts the following technical scheme: a grafting propagation method of the endangered species Fumin trifoliate orange seedlings of the present invention comprises the following steps: (1) selecting a mature common trifoliate orange with many fruits, strong growth, and no diseases and insect pests as a mother tree for seedling cultivation; grafting a seedling with strong growth, no diseases, and a base diameter of more than 0.5 cm as a rootstock; (2) Preparation of scion; (3) Steps of grafting; (4) Management after grafting.

[0006] Furthermore, in step (1), the leaf thorns within 10 cm from the ground are wiped off, and 1-2 year-old branches with no pests and diseases, full buds, and a thickness of 0.4-0.6 cm are cut as scions; a straight surface is selected at 5-10 cm from the ground to cut the head, and all buds and thorns are removed at the same time; the lower end of the scion is cut into a 3-5 cm inclined surface, and after reaching the wood, it is cut straight down, and then the lower end of the back is cut into a 0.5-1.0 cm inclined surface, and the lower end of the scion is sharpened; a vertical cut is made at the straight part of the upper end of the rootstock, reaching the wood, and the length is equivalent to the long cut surface of the scion; then the long cut surface of the scion is inserted into the wood of the rootstock along the incision until it is slightly exposed, so that the cambium on both sides of the scion is closely attached to the inner bark of the rootstock; a single layer of film tape is used to completely seal and wrap it from bottom to top, and then the rootstock and the scion are buried in the soil bag with wet soil.

[0007] Furthermore, in step (2), select 1-2 year old branches with full buds, no pests and diseases, and a thickness of 0.4-0.6 cm as scions. When picking the branches, soak them in a bucket of water for 24 hours to ensure that the scions have sufficient moisture. When grafting, remove the scions 30 minutes in advance and place them in a cool place indoors. During grafting, there should be no moisture at the grafting site to avoid bacterial infection of the site.

[0008] Furthermore, in step (2), 1-2 year old spring buds are selected instead of autumn buds, which have a lower germination rate. Branches with full buds, no diseases or insect pests, and a thickness of 0.4-0.6 cm are used as scions. When picking the branches, soak them in a bucket of clean water for 24 hours to ensure that the scions have sufficient moisture. When grafting, take out the scions thirty minutes in advance and place them in a cool place indoors. No moisture should be allowed at the grafting site during grafting.

[0009] Furthermore, in step (3), a healthy, disease-free, common trifoliate orange seedling with a base diameter of more than 0.5 cm is selected as the rootstock for grafting; leaf thorns are removed, and 1-2 year-old branches with no disease or insect pests, full buds, and a diameter of 0.4-0.6 cm are cut as scions; a straight surface is selected at 5-10 cm from the ground to cut the head, and all buds are removed at the same time; the lower end of the scion is cut into a large bevel of 2-3 cm, and after reaching the wood, it is cut straightly, and then the lower end of the back is cut into a 0.5-1.0 cm bevel. Use a small bevel to sharpen the lower end of the scion; make a vertical cut on the straight part of the upper end of the stock, deep into the wood, and the length is equal to the long cut surface of the scion; then insert the long cut surface of the scion into the wood along the incision until it is slightly exposed, leaving 0.3cm of air permeability on the cut surface of the inserted scion, so that the cambium on both sides of the scion is tightly attached to the inner bark of the stock. If the scion and the stock are not the same thickness, at least one side of the cambium is tightly attached to the inner bark of the stock; use a single layer of film tape to completely seal it from bottom to top, and then plant it in a greenhouse with wet soil.

[0010] Furthermore, in step (4), the management after grafting is as follows: check the germination of the common trifoliate orange, and promptly remove the suckers on the rootstock to prevent them from competing for nutrients with the new grafts; when new shoots begin to sprout on the 25th day, promptly cut a small opening for the new shoots; loosen the film 9 months after survival, and remove all the wrappings after one year; and tie the trunk and branches in time when the new shoots grow to 30-40cm.

[0011] Furthermore, in step (4), the management after grafting is as follows: maintain the temperature of the greenhouse at 30°C, water thoroughly every other day, check the germination of the scion, and promptly remove the suckers on the rootstock to prevent them from competing for nutrients with the new graft; new buds begin to sprout on the 25th day, and windows are opened in time for ventilation of the new buds. After 9 months of survival, the film is loosened, and all the wrapping is removed after one year; when the new shoots grow to 30-40cm, they are promptly tied to protect the branches.

[0012] Beneficial effects: The grafting method provided by the present invention uses common trifoliate orange with strong adaptability and stress resistance as the rootstock for grafting. The grafted Fumin trifoliate orange seedlings have a high survival rate and strong disease and insect resistance, which improves the stress resistance and adaptability of Fumin trifoliate orange. The technical problem of low survival rate of Fumin trifoliate orange during transplantation (return) is effectively overcome. The advantages of the present invention are that it can effectively maintain the survival rate of Fumin trifoliate orange reproduction, solve the problem of fewer fibrous roots of Fumin trifoliate orange, significantly improve the survival rate of transplantation, and have low breeding costs. It is the first artificial grafting seedling raising method for Fumin trifoliate orange established, which is of great significance to the rescue protection and reconstruction of wild populations of Fumin trifoliate orange. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0014] Figure 1 This is a diagram of pruning 1-2 year old scion of Fumin trifoliate orange in Example 1 of the present invention; Figure 2 This is a diagram of the treatment of the Fumin trifoliate orange scion soaked for 24 hours in Example 1 of the present invention; Figure 3 This is a diagram of the shade-drying treatment of the Fumin trifoliate orange scion in Example 1 of the present invention; Figure 4 The Fumin trifoliate orange scion in Example 1 of the present invention is cut into a large inclined plane of 2.5 cm; Figure 5 This is a preparation diagram of the Fumin trifoliate orange rootstock in Example 1 of the present invention; Figure 6 The grafted and bandaged Fumin trifoliate orange diagram in Example 1 of the present invention; Figure 7 This is the grafted and surviving Fuminci trifoliata in Example 1 of the present invention. DETAILED DESCRIPTION

[0015] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application more clearly understood, the present application is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0016] In this application, the term "and / or" describes the association relationship of associated objects, indicating that there may be three relationships. For example, A and / or B can mean: A exists alone, A and B exist at the same time, and B exists alone. A and B can be singular or plural. The character " / " generally indicates that the associated objects are in an "or" relationship.

[0017] In this application, "at least one" means one or more, and "plurality" means two or more. "At least one of the following" or similar expressions refers to any combination of these items, including any combination of single items or plural items. For example, "at least one of a, b, or c", or "at least one of a, b, and c" can all mean: a, b, c, ab (i.e., a and b), ac, bc, or abc, where a, b, and c can be single or multiple.

[0018] It should be understood that in the various embodiments of the present application, the size of the serial numbers of the above-mentioned processes does not mean the order of execution, some or all of the steps can be executed in parallel or sequentially, and the execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of the present application.

[0019] The terms used in the embodiments of the present application are only for the purpose of describing specific embodiments, and are not intended to limit the present application. The singular forms "a", "said" and "the" used in the embodiments of the present application and the appended claims are also intended to include plural forms, unless the context clearly indicates other meanings.

[0020] The weight of the relevant components mentioned in the embodiment description of the present application can not only refer to the specific content of each component, but also indicate the proportional relationship between the weights of the components. Therefore, as long as the content of the relevant components is proportionally enlarged or reduced according to the embodiment description of the present application, it is within the scope disclosed in the embodiment description of the present application. Specifically, the mass described in the embodiment description of the present application can be a mass unit known in the chemical industry such as µg, mg, g, kg, etc.

[0021] The terms "first" and "second" are used only for descriptive purposes to distinguish objects such as substances from each other, and should not be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. For example, without departing from the scope of the embodiments of the present application, the first XX may also be referred to as the second XX, and similarly, the second XX may also be referred to as the first XX. Thus, features defined as "first" and "second" may explicitly or implicitly include one or more of the features.

[0022] Example 1

[0023] like Figures 1 to 7As shown in the figure, a grafting propagation method of the endangered species Fumin trifoliate orange seedlings of the present invention comprises the following steps: (1) selecting a mature common trifoliate orange with many fruits, strong growth and no diseases and insect pests as a mother tree for seed cultivation; selecting a healthy, disease-free seedling with a base diameter of more than 0.5 cm as the rootstock for grafting; erasing leaf thorns within 10 cm from the ground, cutting out two-year-old branches without diseases and insect pests, with full buds and a thickness of 0.6 cm as scions; selecting a flat surface cut 5 cm from the ground, and removing all Cut the lower end of the scion into a large bevel of 3 cm, cut it straight after reaching the wood, and then cut it into a small bevel of 1.0 cm at the lower end of the back, and sharpen the lower end of the scion; make a vertical cut on the straight part of the upper end of the stock, reaching the wood, and the length is equal to the long cut surface of the scion; then insert the long cut surface of the scion into the wood along the incision until it is slightly exposed, so that the cambium on both sides of the scion is tightly attached to the inner bark of the stock; use a single layer of film tape to completely seal it from bottom to top, and then bury the stock and scion in the soil bag with wet soil.

[0024] (2) Preparation of scion: Select 2-year-old branches with full buds, no pests and diseases, and a diameter of 0.6 cm as scion. When picking the branches, put them in a bucket of water and soak them for 24 hours to ensure that the scion has sufficient moisture. When grafting, remove the scion 30 minutes in advance and place it in a cool place indoors. There should be no moisture at the grafting site to avoid bacterial infection.

[0025] (3) Grafting steps: Choose healthy, disease-free, common trifoliate orange seedlings with a base diameter of more than 0.5 cm as rootstocks; remove leaf thorns, and cut out two-year-old branches with no disease or insect pests, full buds, and a diameter of 0.6 cm as scions; select a straight surface 10 cm above the ground to cut the head, and remove all buds at the same time; cut the lower end of the scion into a large bevel of 3 cm, cut straight down after reaching the wood, and then cut a small bevel of 1.0 cm at the lower end of the back, and sharpen the lower end of the scion. End; make a vertical cut on the straight part of the upper end of the rootstock, deep into the wood, and the length is equal to the long cut surface of the scion; then insert the long cut surface of the scion into the woody part along the incision until it is slightly exposed, leaving 0.3cm of air permeability on the cut surface of the inserted scion, so that the cambium on both sides of the scion and the inner bark of the rootstock are tightly together. If the scion and the rootstock are of different thickness, at least one side of the cambium is tightly attached to the inner bark of the rootstock; use a single layer of film tape to completely seal it from bottom to top, and then plant it in the greenhouse with wet soil in time.

[0026] (4) Management after grafting. Management after grafting: Check the germination of common trifoliate oranges in a timely manner, and promptly remove the suckers on the rootstock to prevent them from competing for nutrients with the new grafts; maintain the temperature of the greenhouse at 30°C, water thoroughly every other day, and check the germination of the scion in a timely manner; new buds will begin to sprout on the 25th day, and small holes should be cut in time for the new buds to allow ventilation. After 9 months of survival, loosen the film and remove all the wrapping materials after one year; tie the trunk and protect the branches in time when the new shoots grow to 30-40cm.

[0027] Example 2

[0028] The difference between Example 2 and Example 1 is that: a grafting propagation method of an endangered species, Fumin trifoliate orange seedlings, of the present invention comprises the following steps: in step (1), selecting a mature common trifoliate orange with many fruits, strong growth, and no diseases and insect pests as a seed mother tree for rootstock cultivation; selecting a seedling with strong growth, no diseases, and a base diameter of more than 0.5 cm as the rootstock for grafting; erasing leaf thorns within 5 cm from the ground, and selecting a flat surface cut at 5 cm from the ground, and removing all sprouts at the same time; cutting out the seedlings without diseases and insect pests, with full buds and a thickness of 0 .4cm one-year-old branch as scion; cut the lower end of the scion into a large bevel of 2cm, cut it straight after reaching the wood, and then cut it into a small bevel of 0.5cm at the lower end of the back, and sharpen the lower end of the scion; make a vertical cut at the straight part of the upper end of the stock, deep into the wood, and the length is equal to the long cut surface of the scion; then insert the long cut surface of the scion into the wood of the stock along the incision until it is slightly exposed, so that the cambium on both sides of the scion is tightly attached to the inner bark of the stock; use a single layer of film tape to completely seal it from bottom to top, and then bury the stock and scion in the soil bag with wet soil.

[0029] In step (2), the scion is prepared; one-year-old branches with full buds, no pests and diseases, and a diameter of 0.4 cm are selected as scions. When picking the branches, they are promptly placed in a bucket and soaked for 24 hours to ensure that the scion has sufficient moisture; when grafting, the scion is removed 30 minutes in advance and placed in a cool place indoors. During grafting, there must be no moisture at the grafting site to avoid bacterial infection of the site.

[0030] In step (3), the grafting step includes: selecting healthy, disease-free, common trifoliate orange seedlings with a base diameter of more than 0.5 cm as rootstocks; removing leaf thorns, cutting out one-year-old branches without disease and insect pests, with full buds and a thickness of 0.4 cm as scions; selecting a straight surface 5 cm above the ground to cut the head, and removing all buds at the same time; cutting the lower end of the scion into a 2 cm large bevel, cutting straight down after reaching the wood, and then cutting the lower end of the back into a 0.5 cm small bevel, and sharpening the graft. The lower end of the scion; make a vertical cut on the straight part of the upper end of the rootstock, deep into the wood, and the length is equal to the long cut surface of the scion; then insert the long cut surface of the scion into the woody part along the incision until it is slightly exposed, leaving 0.3cm of air permeability on the cut surface of the inserted scion, so that the cambium on both sides of the scion is tightly attached to the inner bark of the rootstock. If the scion and the rootstock are of different thickness, at least one side of the cambium is tightly attached to the inner bark of the rootstock; use a single layer of film tape to completely seal it from bottom to top, and then plant it in the greenhouse with wet soil in time.

[0031] In step (4), management after grafting. Management after grafting: timely check the germination of common trifoliate oranges, promptly remove the suckers on the rootstock to prevent them from competing for nutrients with the new grafts; maintain the temperature of the greenhouse at 30°C, water thoroughly every other day, and timely check the germination of the scion. New buds will begin to sprout after 25 days, and windows should be opened in time for ventilation of the new buds. After 9 months of survival, the film should be loosened, and all the wrapping materials should be removed after one year; when the new shoots grow to 30-40cm, they should be tied to the trunk and branches in time.

[0032] Example 3

[0033] The difference between Example 3 and Example 1 is that: The invention discloses a grafting propagation method for seedlings of the endangered species Fumin trifoliate orange, comprising the following steps: in step (1), selecting a mature common trifoliate orange with many fruits, strong growth and no diseases and insect pests as a mother tree for seed cultivation as a rootstock; selecting a seedling with strong growth, no diseases and a base diameter of more than 0.5 cm as a rootstock for grafting; removing leaf thorns within 10 cm from the ground, cutting out three-year-old branches without diseases and insect pests, with full buds and a thickness of 0.5 cm as scions; selecting a flat surface cutoff 8 cm from the ground, and removing all the branches at the same time. Cut the lower end of the scion into a large bevel of 2.5 cm, cut it straight after reaching the wood, and then cut it into a small bevel of 0.5 cm at the lower end of the back, and sharpen the lower end of the scion; make a vertical cut on the straight part of the upper end of the stock, reaching the wood, and the length is equal to the long cut surface of the scion; then insert the long cut surface of the scion into the wood of the stock along the incision until it is slightly exposed, so that the cambium on both sides of the scion is tightly attached to the inner bark of the stock; use a single layer of film tape to completely seal it from bottom to top, and then bury the stock and scion in the soil bag with wet soil.

[0034] In step (2), the preparation of the scion; select 3-year-old branches with full buds, no diseases and insect pests, and a thickness of 0.5 cm as scions. When picking, put the branches in a bucket of water and soak for 24 hours in time to ensure that the scion has sufficient moisture; when grafting, remove the scion 30 minutes in advance and place it in a cool place indoors. When grafting, there must be no moisture at the grafting site to avoid bacterial infection of the site. Select 3-year-old spring buds, not autumn buds, which have a lower germination rate. Select branches with full buds, no diseases and insect pests, and a thickness of 0.5 cm as scions. When picking, put the branches in a bucket of clean water and soak for 24 hours in time to ensure that the scion has sufficient moisture; when grafting, remove the scion 30 minutes in advance and place it in a cool place indoors. There must be no moisture at the grafting site.

[0035] In step (3), the grafting step: select healthy, disease-free, common trifoliate orange seedlings with a base diameter of more than 0.5 cm as rootstocks; remove leaf thorns, cut out three-year-old branches without disease and insect pests, with full buds and a thickness of 0.5 cm as scions; select a straight surface 8 cm above the ground to cut the head, and remove all buds at the same time; cut the lower end of the scion into a large bevel of 2.5 cm, cut straight after reaching the wood, and then cut the lower end of the back into a small bevel of 0.8 cm, and sharpen it. The lower end of the scion; make a vertical cut on the straight part of the upper end of the stock, deep into the wood, and the length is equal to the long cut surface of the scion; then insert the long cut surface of the scion into the wood along the incision until it is slightly exposed, leaving 0.3cm of air permeability on the cut surface of the inserted scion, so that the cambium on both sides of the scion and the inner bark of the stock are tightly together. If the scion and the stock are not the same thickness, at least one side of the cambium is tightly attached to the inner bark of the stock; use a single layer of film tape to completely seal it from bottom to top, and then plant it in the greenhouse with wet soil in time.

[0036] In step (4), management after grafting. Management after grafting: timely check the germination of common trifoliate oranges, promptly remove the suckers on the rootstock to prevent them from competing for nutrients with the new grafts; maintain the temperature of the greenhouse at 30°C, water thoroughly every other day, and timely check the germination of the scion. New buds will begin to sprout after 40 days, and windows should be opened in time for ventilation. After 9 months of survival, the film should be loosened, and all the wrapping materials should be removed after one year; when the new shoots grow to 30-40cm, they should be tied to the trunk and branches in time.

[0037] Test Example 1 The Fumin trifoliate orange grafting test data obtained in Example 1 were recorded, and the Fumin trifoliate orange grafting test data record table (different scion control test) is shown in Table 1: Grafting time: 2016.12.20-12.24 Unit: plant

[0038] Table 1 explains: 2-3 year old seedlings of common trifoliate orange were used as rootstocks, and 1-year, 2-year, 3-year, and 4-year old Fumin trifoliate orange scions were grafted, 500 plants of each variety were grafted, and the time and number of new buds of each variety were observed. From the first plant, the first two months were observed and recorded once a week, and from the third month of germination, the observation and statistics were made once a month. The grafting survival rate was calculated from the grafting to the full nine months. The survival rate of 1-year-old Fumin trifoliate orange scion grafted with common trifoliate orange was 45%, the survival rate of 2-year-old Fumin trifoliate orange scion grafted with common trifoliate orange was 68.2%, the survival rate of 3-year-old Fumin trifoliate orange scion grafted with common trifoliate orange was 24.4%, and the survival rate of 4-year-old Fumin trifoliate orange scion grafted with common trifoliate orange was 10.4%.

[0039] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A grafting propagation method for the endangered species Fumin trifoliate orange seedlings, characterized in that The method comprises the following steps: (1) selecting a mature common trifoliate orange tree with abundant fruits, robust growth, and no diseases or insect pests as a rootstock for seed collection; and selecting a robust, disease-free, and disease-free seedling with a base diameter of more than 0.5 cm as a rootstock for grafting; (2) Preparation of scion; (3) Steps of grafting; (4) Management after grafting.

2. The grafting propagation method of the endangered species Fumin trifoliate orange seedlings according to claim 1, characterized in that: In step (1), remove leaf thorns within 10 cm from the ground, cut out 1-2 year old branches with no pests and diseases, full buds and a thickness of 0.4-0.6 cm as scions; select a straight surface 5-10 cm from the ground to cut the head, and remove all buds and thorns at the same time; cut the lower end of the scion into a 3-5 cm inclined surface, cut straight after reaching the wood, and then cut a 0.5-1.0 cm inclined surface at the lower end of the back, and sharpen the lower end of the scion; make a vertical cut at the straight part of the upper end of the stock, deep into the wood, and the length is equal to the long cut surface of the scion; then insert the long cut surface of the scion into the wood along the incision until it is slightly exposed, so that the cambium on both sides of the scion is closely attached to the inner bark of the stock; use a single layer of film tape to completely seal and wrap from bottom to top, and then bury the stock and scion in the soil bag with wet soil.

3. The grafting propagation method of the endangered species Fumin trifoliate orange seedlings according to claim 1, characterized in that: In step (2), select 1-2 year old branches with full buds, no pests and diseases, and a diameter of 0.4-0.6 cm as scions. When picking the branches, soak them in a bucket of water for 24 hours to ensure that the scions have sufficient moisture. When grafting, remove the scions 30 minutes in advance and place them in a cool place indoors. No moisture should be allowed at the grafting site during grafting.

4. The grafting propagation method of the endangered species Fumin trifoliate orange seedlings according to claim 3, characterized in that: In step (2), select 1-2 year old spring buds with full buds, no diseases or insect pests, and a diameter of 0.4-0.6 cm as scions. When picking the branches, soak them in a bucket of clean water for 24 hours to ensure that the scions have sufficient moisture. When grafting, take out the scions 30 minutes in advance and place them in a cool place indoors. No moisture should be allowed at the grafting site during grafting.

5. The grafting propagation method of the endangered species Fumin trifoliate orange seedlings according to claim 1, characterized in that: In step (3), a healthy, disease-free, common trifoliate orange seedling with a base diameter of more than 0.5 cm is selected as the rootstock for grafting; leaf thorns are removed, and 1-2 year-old branches with no disease or insect pests, full buds, and a diameter of 0.4-0.6 cm are cut as scions; a straight surface is selected 5-10 cm above the ground to cut the head, and all buds are removed at the same time; the lower end of the scion is cut into a large bevel of 2-3 cm, and after reaching the wood, it is cut straightly, and then the lower end of the back is cut into a small bevel of 0.5-1.0 cm. Cut a vertical cut in the straight part of the upper end of the stock, deep into the wood, and equal in length to the long cut surface of the scion; then insert the long cut surface of the scion into the wood along the incision until it is slightly exposed, leaving 0.3cm of air permeability on the cut surface of the inserted scion, so that the cambium on both sides of the scion is tightly attached to the inner bark of the stock. If the scion and the stock are of different thickness, at least one side of the cambium is tightly attached to the inner bark of the stock; use a single layer of film tape to completely seal it from bottom to top, and then plant it in a greenhouse with wet soil.

6. The grafting propagation method of the endangered species Fumin trifoliate orange seedlings according to claim 1, characterized in that: In step (4), management after grafting: check the germination of common trifoliate oranges and remove the suckers on the rootstock; new buds begin to sprout after 25 days, cut a small hole for the new buds, loosen the film 9 months after survival, and remove all the wrappings after one year; tie the trunk to protect the branches when the new shoots grow to 30-40cm.

7. The grafting propagation method of the endangered species Fumin trifoliate orange seedlings according to claim 6, characterized in that: In step (4), the management after grafting is as follows: maintain the temperature of the greenhouse at 30°C, water thoroughly every other day, check the germination of the scion, and remove the suckers on the rootstock; new buds will begin to sprout on the 25th day, open windows for ventilation, loosen the film 9 months after survival, and remove all the wrappings after one year; tie the trunk and protect the branches when the new shoots grow to 30-40cm.

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