282 results about "Zooid" patented technology

The invention provides a fully-artificial breeding method of parent crabs of Portunus trituberculatus, comprising the following steps of: selecting large female and male parents with good characters; artificially controlling the female and male parents to be mated; numbering the mated female crabs and then putting into an overwintering pond, wherein baits of an overwintering period are living clam worms; utilizing sand-filtered underground seawater and controlling the water temperature in a range of 8-10 DEG C to accelerate sexual glands to grow to be mature and ovulate; putting one parent crab with ova into a cage and then putting the cage into a breeding pond to ovulate larvae and breed in the May of the next year; culturing the larvae in one pond to the second phase of young crabs; then putting the young crabs into a cultivation pond to be cultivated, so as to realize the fully-artificial cultivation of the Portunus trituberculatus. According to the invention, the fights and injuries of male crabs are reduced and the parent breeding success rate is improved; the generation and the transmission of diseases are reduced, and the overwintering survival rate is improved; and meanwhile, the fully-artificial breeding method is also applicable to temporary cultivation of commodity crabs so that the marketing period of the commodity crabs is prolonged and the economic additional value of the cultivated crabs is improved; and the larvae are cultivated in one pond so that the fully-artificial breeding method is applicable to propagating of the population of the Portunus trituberculatus breeding population.

The physiological regulation of intake, growth and energy partitioning in animals is under the control of multiple genes, which may be important candidates for unraveling the genetic variation in economically relevant traits in beef production. The present invention relates to the identification of single nucleotide polymorphisms (SNPs) within the bovine gene encoding mitochondrial transcription factor A (“TFAM”) and their associations with economically relevant traits in beef production. The invention further encompasses methods and systems, including network-based processes, to manage the SNP data and other data relating to specific animals and herds of animals, veterinarian care, diagnostic and quality control data and management of livestock which, based on genotyping, have predictable meat quality traits, husbandry conditions, animal welfare, food safety information, audit of existing processes and data from field locations.

The invention relates to a breeding method for a rapid growing line of seawater pearl shells, which comprises the steps of: taking a certain population of Pinctada martensii Dunker as a basic population, firstly selecting individuals with shell width being 10-40 percent higher than average value from the basis population by taking shell width as a selection index, then selecting individuals with shell height or weight being 10-60 percent higher than average value as breeding parents, dissecting the breeding parents, taking sexual glands to conduct artificial insemination, selecting and reserving preparative parents at a young shell stage according to a proportion of 20-30 percent, and finally conducting more than three generations of continuous selection to obtain the rapid-growing large excellent Pinctada martensii Dunker line (variety). The breeding method has the advantages that the method is simple, convenient and feasible, the cost for nursing the parents can be reduced, the growth speed and the individual size of the Pinctada martensii Dunker can be effectively improved and the pearl producing effect is improved.

The invention provides a mitochondrial gene sequence for identifying authenticity and quality of Cordyceps sinensis and an operation method thereof. According to the method, differences of mitochondrial genes between ghostmoth and other lepidopteran insects are compared and thus high-variability areas can be searched. Specific primers of the ghostmoth are designed to obtain sequences of corresponding sections of mitochondria of host insects; if a nucleotide difference rate is less than 1-1.5 %, the insects are determined to be a same species but different individuals, and if the nucleotide difference rate is more than 6 %, the insects are determined to be a same genus but different species, thereby not only identifying whether to-be-detected samples are the Cordyceps sinensis formed with Hepialus larvae as the host insects, but also determining producing areas of the to-be-detected samples, and thus determining the authenticity and grades of the to-be-detected samples The method is simple in steps, easy to operate, and few in sample amount, and can minimize sample loss. According three pairs of the specific primers provided by the invention, the authenticity and the grades of the samples can be rapidly and conveniently detected through detecting whether the samples have same or similar nucleotide sequences as well as similarity of the nucleotide sequences.

The invention discloses a pure breeding method for soft-shelled turtles. Soft-shelled turtle fries, soft-shelled turtles cultured in an outdoor storage pond, soft-shelled turtles cultured in a factory greenhouse and fertilized eggs of the soft-shelled turtles are purified, rejuvenated and selected for many times by multiple steps, and meanwhile, each culture condition is controlled; the soft-shelled turtles are multi-generationally bred and cyclically selected. According to the method, a temperature and periodical humidity combination control method is adopted for an artificial incubation process of the fertilized eggs, the purity of second-generation soft-shell turtle generations purely bred by the method reaches more than 98 percent, the purity of third-generation soft-shell turtle generations purely bred by the method is higher than 99.5 percent, and the shape similarity, the uniformity, the resilience, the growth speed and the yield per unit of soft-shelled turtle individuals are remarkably improved.

The invention discloses a PCR primer pair used for identifying the genetic sex of Pseudobagrus ussuriensis, and a rapid identification method thereof. The identification method comprises the steps of examined individual tissue drawing, genome DNA extraction, sex specific DNA fragment PCR amplification, agarose gel electrophoresis, ultraviolet light detection shooting and examined individual genetic sex determination. High-quality genome DNA is extracted through an optimized process, a sex specific DNA fragment is amplified, and the existence or not and the existence characteristic of the sex specific fragment are detected under ultraviolet lights, so the genetic constitution of Pseudobagrus ussuriensis is identified, and the genetic sex is discriminated in 1-2d. The PCR primer pair and the rapid identification method provide a rapid identification method for identifying sex reversal fish and super-male fish, provide technical support for breeding all-male fish, and have the advantages of breeding time shortening, great increase of the output, breeding cost reduction, and profound economic and social values.

The physiological regulation of intake, growth and energy partitioning in animals is under the control of multiple genes, which may be important candidates for unraveling the genetic variation in economically relevant traits in beef production. The present invention relates to the identification of single nucleotide polymorphisms (SNPs) within the bovine genes encoding fatty acid binding proteins and their associations with economically relevant traits in beef production. The invention further encompasses methods and systems, including network-based processes, to manage the SNP data and other data relating to specific animals and herds of animals, veterinarian care, diagnostic and quality control data and management of livestock which, based on genotyping, have predictable meat quality traits, husbandry conditions, animal welfare, food safety information, audit of existing processes and data from field locations.

The present disclosure relates to a system and a method for identification of individual animals based on images, such as 3D-images, of the animals, especially of cattle and cows. When animals live in areas or enclosures where they freely move around, it can be complicated to identify the individual animal. In a first aspect the present disclosure relates to a method for determining the identity of an individual animal in a population of animals with known identity, the method comprising the steps of acquiring at least one image of the back of a preselected animal, extracting data from said at least one image relating to the anatomy of the back and/or topology of the back of the preselected animal, and comparing and/or matching said extracted data against reference data corresponding to the anatomy of the back and/or topology of the back of the animals with known identity, thereby identifying the preselected animal. The method and system can be used to monitor feed intake, such as feed intake for dairy cows as well as health status.

The present invention provides computer-assisted methods, including interactive systems, for tracking and correlating of breeding, veterinary care and performance data relating to livestock animals. In particular, the methods are especially useful for tracking breeding and veterinary care histories of individual animals or the collective herd or flock and relating the histories to performance parameters of the animals and to expected or desired performances. The methods of the invention can allow the operator to be alerted by deviation of the actual performance parameters from desired performances so that feed, medications, vaccinations, husbandry conditions, food safety etc of the animals may be adjusted or corrected accordingly. Such methods include obtaining and maintaining the data obtained from each animal, and optionally other data relating to the animal's health, condition or parentage, or to its herd, and providing this data to others through web-based systems, contained in a database, or attached to the animal itself such as by an implanted microchip.

The invention discloses a method for breeding a sipunculus nudus parent. Artificial breeding of sipunculus nudus is high in cost, very instable in effect and significantly relevant to poor artificial spawning effect, high mortality and inconsistent egg laying quality of the sipunculus nudus parent. Meanwhile, mismatch of the yield is caused due to excessive concentration of egg laying in induced spawning, leading to resource waste. By adopting the measures of building of a sipunculus nudus parent breeding pond, indoor artificial food culture, maturing and breeding management, disease control and prevention and artificial control of egg laying, the mortality is lowered to 7-12 percent within 40 days of artificial indoor parent maturing and egg laying breeding, the single yield of an individual parent of 20g is 50,000-70,000 eggs, the metamosphsis rate of hatched larvae is 13-86 percent, and artificial control of egg laying can be performed according to the production capacity. By adopting the method, the source and controllability of fertilized eggs are realized in plant artificial breeding and selective breeding of the sipunculus nudus, the egg laying time of the parent is prolonged, and the breeding cost is saved.

The invention belongs to the field of fish sex determination in the field of aquaculture, and particularly relates to a specific DNA fragment SSM2 for sex determination of sturgeons and application. According to the invention, a specific DNA fragment of sturgeon female individuals is obtained through a high-throughput sequencing and genome comparison method. Primers are designed for the fragment,and can perform accurate and rapid sex identification on nine kinds of sturgeons. An identification method of the invention has the characteristics of simple, quick and accurate operation, extremely small damage to the sturgeons and the like, solves the problem of sturgeon sex identification, and is helpful for rapid development of the sturgeon culture industry.

The invention relates to an artificial breeding method for Stichopus japonicus, belonging to the field of sea cucumber breeding. The research reports on the artificial breeding of the sea cucumber at home and abroad are very few at present and the artificial breeding technique lacks for systematic information. The invention systematically introduces notable problems of all links from the aspects of breeding facilities, water quality conditions, algae seed selection, bait cultivation and the like. Especially for the fishing of parent sea cucumbers, undamaged individuals with body length being above 20cm, body weight being above 250g and gonad indexes being above 10 percent are required to be selected as the parent sea cucumbers; and for larva breeding, breeding plants mostly adopt a shade-drying running water stimulation method to induce spawning, and the fed baits are mainly cultivated algae which comprise Chaetoceros muelleri, Phaeodactylum tricomutum, Nitzschia closterium Minutissima and the like. By controlling breeding density and well doing all links such as spawning, hatching and larva culture, a good effect is achieved.

The invention provides a scale estimation method for a spawning ground of river fishes capable of laying adhesive eggs. According to the scale estimation method, a spawning ground scale T is estimated according to an investigation object population total number S, a sexual maturity individual number ratio P1, a reproductive population female number ratio P2, a after-egg laying individual ratio P3 and a relative fish brood amount R; and the spawning ground scale T is equal to S*P1*P2*P3*w*R. According to the method provided by the invention, fishing investigation is combined with fish population growth and death parameter estimation, and reproductive biology parameters; and the scale estimation problem of the spawning ground of the river fishes capable of laying the adhesive eggs is solved, so that the difficulties that area of an egg laying region and fish egg density and the like underwater are difficult to count, and equipment cannot develop the work while the equipment does not meet the requirements are avoided.

The invention provides a triops sinensis imitating ecological breeding method. The method comprises the following steps of egg incubation and larva breeding, adult breeding, egg laying and collection, and daily management. The method comprises the steps that aquatic plant and plant debris with beneficial microorganism are utilized for building a micro ecological system for triops sinensis, the micro ecological system can provide food for the triops sinensis and can purify water quality of itself, special buoyancy bait is fed, the water temperature is kept constant, and oxygenation is kept for 24 hours, a breeding box is utilized for collecting eggs, and annual indoor breeding of triops sinensis can be achieved. Adult triops sinensis bred through the method is robust and high in disease resistance, the female adult triops sinensis can lay 400-500 eggs on average, the hatchability is 89%, the larval survival rate is 92%, the breeding method is simple, the breeding cost is low, the method can be used for manually increasing the population number of the triops sinensis, and the triops sinensis imitating ecological breeding method is of great significance in protecting, researching, developing and utilizing rare animals, and has higher economic benefits and ecological benefits.

The invention relates to the field of the aquiculture, in particular to methods of the breed selection of the soft-shelled turtle breed-parent and the breeding of the soft-shelled turtle. The breed selection of the soft-shelled turtle includes obtaining F1 by crossbreeding of the soft-shelled turtle individual and the strip-necked turtle; obtaining the soft-shelled turtle breed-parent F4 after breed selection of three generations, the genetic character thereof is stable at above 99%. The invention also discloses the breeding of the soft-shelled turtle, which includes introducing and breeding F4 as the breed-parent; egg-laying, egg taking, incubating, temporarily breeding of young turtles, breeding of baby turtles, stocking in the outer pond and obtaining the soft-shelled turtle. The bred soft-shelled turtle is characterized in good taste, good mouth feeling and high nutritive value.

The invention discloses an animal body temperature monitoring system and a machine learning based body temperature state recognition method. The monitoring system includes an acquisition device and an intelligent terminal application; the acquisition device can acquire a contact pressure, the body temperature, and the acceleration information of an animal to be monitored, and can determine if there is an effective contact or not according to the contact pressure; and the intelligent terminal application receives effective animal data of the acquisition device, and recognizes the effective animal data as an animal body temperature state. The recognition method can learn and acquire the normal body temperature of the animal to be monitored in different motion states, and can recognize the body temperature state of the animal on the basis of learned information. The monitoring system can achieve automatic detection and remote real-time monitoring of the animal body temperature without manual detection or records, and can save lots of manpower. The recognition method can eliminate differences in species, ages, individuals and motion states of the animals, is really customized for each object to be monitored, and can achieve accurate and effective body temperature monitoring.

The invention relates to an animal counting and image processing method and device. The method comprises the steps: obtaining a to-be-detected region image which comprises at least one animal; segmenting animal individuals from the to-be-detected area image according to a pre-trained animal detection model, and identifying the number of animals; and generating counting information corresponding tothe to-be-detected area image according to the animal number. According to the technical scheme, the animal number is identified from the to-be-detected area image through the pre-trained animal detection model, so that intelligent automatic counting is realized, manual counting is not needed, the counting efficiency is improved, the accuracy and reliability are relatively high, and the manpowerand time costs are saved.

The invention belongs to the technical field of aquatic product biology and discloses a molecular marker for genetic sex identification of Chinese soft-shell turtles and a method. The method comprisesthe steps: sequencing male and female individuals of the Chinese soft-shell turtles by adopting a RAD technology, and screening male and female specific fragments; carrying out female genome Walkingaccording to the male and female specific fragments, so as to obtain a specific fragment SEQ ID NO: 1 of 6532bp; designing primers on the basis of the specific fragment, and carrying out primer screening and verification, so as to obtain specific primers; acquiring calipash tissue of the Chinese soft-shell turtles, extracting genomic DNA from the Chinese soft-shell turtles, and carrying out amplification on Chinese soft-shell turtle individuals by using primer pairs; subjecting an amplification result to cataphoresis detection; carrying out genetic sex identification. According to the molecular marker and the method, the problem that gender-specific markers of the Chinese soft-shell turtles are not applied to genetic sex identification yet at present is solved, and thus, the molecular marker and the method are original. The success rate of accurate identification reaches 100%.

The invention discloses a breeding method for maintaining the excellent traits of Procambarus clarkii. The method belongs to the field of animal molecular breeding, and specifically relates to a breeding method for maintaining the excellent traits of Procambarus clarkii. Through parental selection and cultivation, establishment of family, hatching and seedling, breeding of a family with excellenttraits, calculating the kinship between different individuals, re-selecting parents to cultivate, the excellent traits of stable Procambarus clarkii can be cultivated after multiple generations of breeding. The method lays a foundation for the construction and breeding of large-scale families, which is helpful for breeding new strains with excellent growth traits.

The invention discloses a detection technology by ptssr36 microsatellite DNA markers in a blue crab. The detection technology is characterized in that: first, genome DNA of the blue crab is extracted and diluted to reserve; second, by utilizing a ptssr36 microsatellite core sequence in a genomic library of the blue crab, specificity primers are designed at two ends of the sequence thereof; third, the genome DNA of different geographic groups of the blue crab or individuals in a blue crab group is processed through the PCR amplification by using the primers, and PCR products are processed through the modified polyacrylamide gel detection; finally, bands which are generated in the products are utilized for analyzing so as to determine the genotype of each individual, thereby obtaining a polymorphic map on the enormous genetic variation of the blue crab in the a ptssr36 core sequence area. The polymorphic map that the ptssr36 genetic mark gene locus of the blue crab shows the enormous genetic variation can be obtained rapidly; the method is simple and convenient; the each individual genotype of the blue crab at the locus can be detected intuitively from the obtained results. The detection technology is mainly used in the genetic marks among the blue crab groups, the genealogical identification, the genetic map construction, etc.

A method for in-lab breeding of Spodoptera frugiperda in winter comprises the steps of (S1) adult pairing, (S2) uniform egg cultivation, (S3) incubation and feeding, (S4) excrement cleaning and feed changing for first to third larvae, (S5) dividing and breeding of advanced-age larvae, (S6) preparation of a pupation environment, and (S7) centralized pupa breeding and worm formation by pupation. Different devices are used in different stages, disposable plastic cups are used during adult pairing and larva breeding, narrow space of the disposable plastic cups is beneficial for adult pairing, andescape of young-age larvae which are small in size and quick in creeping can be prevented through cup breeding; and a latticed breeding device is used for the advanced-age larva, so that mutual bitingcaused by competition can be avoided, space is saved, and the larvae of different ages can be bred at the same time. The method for in-lab breeding of Spodoptera frugiperda in winter effectively increases a survival rate, is high in breeding density and small in floor area, increases the yield, is convenient to operate and saves time and effort.

The invention discloses an animal snarling monitoring system and a snarling state recognition method based on machine learning. The animal snarling monitoring system comprises an acquisition device and an intelligent terminal application, and the acquisition device acquires contact pressure, vocal cord state and snarling information of monitored animals and judges whether effective contact occursor not according to the magnitude of the contact pressure; the intelligent terminal application receives effective animal data of the acquisition device and recognizes the effective animal data into the animal snarling state. By means of the recognition method, the normal snarling state of monitored animals in different motion states can be obtained through learning, and based on the learnt information, the snarling state of the animals can be recognized. By means of the system, animal snarling can be automatically detected and remotely monitored in real time, manual detection or recording isnot needed, and a lot of manpower can be saved; by means of the method, the difference like the type, the age, the individual and the motion state of the animals can be eliminated, all monitored objects are truly customized, and the purpose of precisely and effectively detecting snarling is achieved.