109 results about "Sipunculus nudus" patented technology

The invention discloses a method for artificially cultivating Sipunculus nudus Linnaeus, which provides an artificially cultivating technical method comprising parent Sipunculus nudus Linnaeus cultivation, artificially induced spawning, planktonic larvae cultivation and naiad cultivation aiming at the problem that Sipunculus nudus Linnaeus cannot be artificially cultivated and produced in large scale currently, and realizes the large scale artificial cultivation of the Sipunculus nudus Linnaeus to satisfy the demands for aquaculture production development.

The invention discloses a method for mixed breeding of Sipunculus nudus and Babylonia areolata. At present, Sipunculus nudus breeding is mudflat breeding without feed, suitable mudflats are limited, and the breeding period is as long as 1.5 years. Bait of Babylonia areolata generally comprises chilled trash fishes, residual bait, residues and metabolic waste, water quality decays easily, and large-scale breeding benefit is not high. Through measures including pond construction, breeding pretreatment, seedling breeding, bait feeding, water quality regulation and disease control, complementary mixed breeding of Sipunculus nudus and Babylonia areolata is realized, and the problem that substrate deterioration caused by high-density breeding and bait feeding in the Babylonia areolata breeding process is solved. The Sipunculus nudus ingests various residues produced after decomposition of residual bait, and the growth speed is greatly increased compared with that through sea area natural extensive breeding. With the adoption of the method, the breeding environment of the Babylonia areolata is improved, the breeding survival rate and quality are improved, the breeding period of the Sipunculus nudus is shortened, the breeding space is saved, and the breeding benefit is increased.

The invention discloses a method for breeding a sipunculus nudus parent. Artificial breeding of sipunculus nudus is high in cost, very instable in effect and significantly relevant to poor artificial spawning effect, high mortality and inconsistent egg laying quality of the sipunculus nudus parent. Meanwhile, mismatch of the yield is caused due to excessive concentration of egg laying in induced spawning, leading to resource waste. By adopting the measures of building of a sipunculus nudus parent breeding pond, indoor artificial food culture, maturing and breeding management, disease control and prevention and artificial control of egg laying, the mortality is lowered to 7-12 percent within 40 days of artificial indoor parent maturing and egg laying breeding, the single yield of an individual parent of 20g is 50,000-70,000 eggs, the metamosphsis rate of hatched larvae is 13-86 percent, and artificial control of egg laying can be performed according to the production capacity. By adopting the method, the source and controllability of fertilized eggs are realized in plant artificial breeding and selective breeding of the sipunculus nudus, the egg laying time of the parent is prolonged, and the breeding cost is saved.

The invention provides a skin wound repairing ointment, which is characterized in comprising the following ingredients in parts by weight: 20-25 parts of wool grease, 40-50 parts of albolene, 20-25 parts of glycerol, 2-6 parts of marine mollusk active peptide, 0.1-0.3 part of collagen peptide and 1-5 parts of water, wherein the marine mollusk active peptide is a marine mollusk mantle enzymolysis product, and the collagen peptide is a sipunculus nudus collagen enzymolysis product. The skin wound repairing ointment has the efficacies on resisting bacteria, diminishing inflammation, removing slough, promoting growth of tissue regeneration, astringing, healing and inhibiting scar hypertrophy and is especially suitable for wound healing and reducing remaining scars.

The invention discloses a breeding method of artificial parents of sipunculus nudus. The sipunculus nudus is low in food chain level and strong in stress resistance, does not migrate in a long distance, and is cultivated without feeding, thereby being suitable for large-area artificial cultivation. By the technical measures of primary selection of parents, natural spawn cultivation, trochophore selection, larva cultivation and selection, cultivation and selection of young sipunculus nudus, parents breeding, and selection of the bred parents, excellent artificial parent selection and volume production of the sipunculus nudus are achieved under the situation of occupying fewer resources; the yield of parent selection is 77%-94%; the mean growth and metamosphsis rate of oosperms produced by the parents is 88%; the abnormal survival rate is 48.3%; the artificial breeding efficiency of the sipunculus nudus is greatly improved, and offspring seeds produced by the selected parents in mudflataquaculture of two years grow faster than wild seeds by 8.3%. According to the invention, the parent selection of the sipunculus nudus is carried out under an artificial control condition; and the breeding method is shorter in time and stable in result, and plays a role in greatly promoting the development of an artificial aquaculture industry of the sipunculus nudus.

The invention discloses a high-yield mudflat cultivation method of Sipunculus nudus. The cultivation method of Sipunculus nudus is simple, low in investment and less risky; traditional cultivation in cultivation sea areas is basically a management-free or low-yield cultivation method. Years of efficient mudflat cultivation tests are performed according to physiological living habits of Sipunculus nudus, the steps such as selecting farm conditions, treating farms, selecting fry sources and specifications, raising fry, managing development and harvesting are taken, intensive high-yield mudflat cultivation of Sipunculus nudus is achieved, the cultivation period is a year and half, yield per mu is 280.0-320.3kg, mudflat cultivation yield per mu of Sipunculus nudus is increased greatly, and mudflat resources are fully utilized. The high-yield mudflat cultivation method of Sipunculus nudus is scientific and reasonable in process and highly operable; high-yield cultivation of Sipunculus nudus is achieved by reasonable and efficient use of mudflats.

The invention relates to an ocean invertebrate plasmin which is a protein obtained by steps of homogenizing an ocean Sipunculus nudus digestive gland fluid, filtering to remove grease, freeze-drying, salting-out, desalinizing and carrying out DEAE chromatography and gel filtration chromatography. Amino acid sequence of the N-terminal part is Seq.2, and molecular weight is 15 KDa. It proves through experiment research that the plasmin has good effects of resisting thrombus and preventing and curing stroke.

The invention discloses a Sipunculus nudus polysaccharide oral liquid. Pharmacological tests confirm that Sipunculus nudus polysaccharide has good inhibition effect on DNA copying of hepatitis B virus, can effectively remove hydroxy free radicals and superoxide anion free radicals to play the role of resisting ageing, and has obvious inhibition effect on growth of staphylococcus aureus, Escherichia coli and bacillus subtilis. The Sipunculus nudus polysaccharide oral liquid provided by the invention is prepared from a Sipunculus nudus polysaccharide liquid, a grosvenor momordica fruit water extract, a red date water extract, a matrimony vine water extract, a haw water extract, a Radix Astragali water extract and a licorice water extract through steps of material dosing, filling and disinfection. The Sipunculus nudus polysaccharide oral liquid maintains the activity of Sipunculus nudus polysaccharide, and has reasonable compatibility and synergism with the six substances including grosvenor momordica fruit, red date, matrimony vine, haw, Radix Astragali and licorice, and is helpful to improving sub-health state of human body. The Sipunculus nudus polysaccharide oral liquid of the invention employs a reasonable, precise and appropriate formula, has suitable acidity and sweetness and pure mouthfeel, and is suitable for drinking by people at every age.

The invention discloses a sipunculus nudus drying device and an application system thereof. A cuboid cabinet structure is adopted for the device, and the device comprises a cabinet body and a cabinet door; the cabinet body is supported by four wheels; the inner side walls of the cabinet body are provided with a plurality of layers of check blocks which are provided with grid plates; the inner side walls of the cabinet body and a base are provided with a plurality of heating pipes; three draught fans are arranged in the base; dehumidifying holes are formed in the upper end of the cabinet body; and a control display screen is arranged on the outer side of the cabinet door. The sipunculus nudus drying device is simple and practicable and high in automation degree, the temperature and the work time can be automatically adjusted according to the weight change of sipunculus nudus in the cabinet, the drying effect is guaranteed, scorches are prevented, large batch of sipunculus nudus can be dried, and the sipunculus nudus drying efficiency is improved. The invention further provides the application system of the sipunculus nudus drying device.

The invention provides a sipunculus nudus workshop culture pond which comprises a pond body. The pond body is a pit comprising a pond wall and a pond bottom, an opening is formed in the upper portion of the pond body, the pond wall and the pond bottom are built with cement or bricks, a filter composite layer is arranged on the upper surface of the pond bottom, a clay brick layer, a filter plate, a bolting-silk net and a sand layer are sequentially arranged on the filter composite layer from bottom to top, clay bricks of the clay brick layer are arranged at intervals, gaps are reserved among the clay bricks, a water inlet pipe is arranged on one side of the pond wall, a filter bag sleeves a pipe orifice of the water inlet pipe, and a drain hole is formed in the pond bottom. Aquaculture water in the pond body can be conveniently and rapidly replaced, poisonous and harmful substances in the sand layer are timely discharged, a substrate environment of a sipunculus nudus cement culture pond is effectively improved, harmful organism breeding is decreased, and siphon-worm survival rate and growth speed are increased. Besides, the culture pond is convenient to build, mount and debug and simple in operation and running maintenance, and has a great application prospect.

The invention applies to the technical field of extraction of effective ingredients from organisms and discloses Sipunculus nudus somatic protein peptide extract and a preparation method thereof, wherein the preparation method comprises the steps of preparing Sipunculus nudus body wall from live Sipunculus nudus; crushing and homogenizing the Sipunculus nudus body wall to obtain Sipunculus nudus body wall homogenate; subjecting the Sipunculus nudus body wall homogenate to ultrasonic cell breaking to obtain an ultrasonically treated liquid; enzymatically hydrolyzing the ultrasonically treated liquid with proteinase to obtain enzymatic hydrolysate; subjecting the enzymatic hydrolysate to enzyme deactivation, and centrifuging to obtain enzymatic hydrolyzed supernate; lyophilizing the enzymatic hydrolyzed supernate to obtain Sipunculus nudus somatic protein peptide extract. The preparation method of the invention has the advantages of high safety, mild production conditions, good convenience of control, good process simplicity, good convenience of industrial production, low cost and the like; the prepared extract has high hydrolytic degree and high protein peptide content.

The invention discloses a factory breeding device for Sipunculus nudus. The actory breeding device for Sipunculus nudus comprises water pipes and a breeding trough, wherein the water pipes comprise awater inlet pipe and a water drainage pipe, two ends of the breeding trough are separately installed on the water inlet pipe and the water drainage pipe, and a water supply pipe is arranged above thebreeding trough; a water release pipe is arranged at the bottom of the breeding trough in a communicating mode, one end of the water supply pipe is installed on the water inlet pipe and communicates with the water inlet pipe, and the other end of the water supply pipe is installed on the water drainage pipe; one end of the water release pipe is installed on the water inlet pipe, the other end of the water release pipe is installed on the water drainage pipe and communicates with the water drainage pipe, and a return pipe is further arranged between the water inlet pipe and the water drainage pipe; and the return pipe is provided with a water quality processor, a fertilizer-water processor and a water temperature processor in sequence. The factory breeding device for Sipunculus nudus, conditions for transition from mudflat breeding and pond breeding of Sipunculus nudus to factory breeding are provided, the problem of environmental limitation is solved, and the factory breeding device for Sipunculus nudus has a high survival rate, a high growth rate, high and stable yield and more convenient fishing when applied to breeding of Sipunculus nudus.

The invention discloses application of sipunculus nudus polysaccharide to preparation of drugs or health care products for prevention and treatment of osteoporosis (OP). Pharmacological experiment research proves that the sipunculus nudus polysaccharide can significantly improve the proliferative activity of preosteoblast MC3T3-E1 cultured in vitro, promotes the preosteoblast MC3T3-E1 to be subjected to differentiation, and can significantly increase the bone mineral density of 10-month years old of mice; and the results show that the sipunculus nudus polysaccharide has the potential of treating or preventing OP. According to the novel purpose of the sipunculus nudus polysaccharide, the indication or the application range of the sipunculus nudus polysaccharide is enlarged, and the sipunculus nudus polysaccharide is expected to be developed into the new drugs or health care products for prevention and treatment of OP.

The invention discloses sipunculus nudus ACE inhibitory peptide as well as a preparation method and application thereof, and belongs to the technical field of biology. Through a single factor experiment and an orthogonal experiment, the optimal process parameters of enzymolysis of sipunculus nudus are determined as compound enzyme powder (the ratio of pancreatin to neutral protease is 3: 1), the material-liquid ratio is 1: 10, the mass ratio of enzyme to a substrate is 1%, the enzymolysis pH is 9.5, the enzymolysis temperature is 45 DEG C, the enzymolysis time is 4h, and an enzymolysis product is separated and purified through metal chelating chromatographic column chromatography. and three polypeptide amino acid sequences are obtained through high performance liquid chromatography-tandem mass spectrometry identification, and a sipunculus nudus antihypertensive peptide buccal tablet is prepared from the obtained ACE inhibitory peptide. The invention provides the new sipunculus nudus antihypertensive peptide, determines an optimal preparation process, and lays a foundation for further preparation of drugs with an antihypertensive effect.

The invention discloses an effervescent tablet containing vitamin C and sipunculusnudus enzymatic protein. The effervescent tablet is prepared from raw materials, namely, the sipunculusnudus enzymatic protein powder, the vitamin C, powdered sugar, citric acid, acesulfame, sodium bicarbonate, magnesium stearate and green tea essence. Main components of the sipunculusnudus enzymatic protein powder are polypeptide substances and amino acids, the sipunculusnudus enzymatic protein powder and the vitamin C can enhance the immunity and the disease resistance of human bodies after absorbed by the human bodies, and after the two components are combined, the vitamin C can activate proline hydroxylase and lysine hydroxylase in the sipunculusnudus enzymatic protein powder and promote proline and lysine to be converted into hydroxyproline and hydroxylysine, so that the absorption and utilization of protein are promoted, and the bioavailability is improved. The effervescent tablet also has the characteristics as follows: the medicine can be quickly disintegrated into fine granules to be quickly dissolved due to the powerful disintegration action; the effervescent tablet is well absorbed in the human bodies and is particularly applicable to children; a medicine liquid is cool and tasty, tastes good and acceptable to people.

The invention discloses a sipunculus nudus family establishing and excellent variety breeding method. As variety degeneration occurs due to an extensive breeding way of sipunculus nudus, the invention adopts the following technical measures: 1, parent conditions and selection: parents are selected to establish half-sib families; 2, optimal selection during a pelagospheric larvae metamorphosis settlement stage: the families of pelagospheric larvae with the metamorphosis survival rate reaching 40% or above are selected as primary selection families; 3, optimal selection during a middle larva breeding stage: 60% of the families with the high growth speed and high survival rate are selected as alternative families; 4, optimal selection during a sexual maturity stage: optimal selection is carried out on the alternative families, and 40% of the families with the high growth speed, high survival rate and relatively high whole sexual maturity are selected as parents; 5, elimination of inferior families: copulation between sibs and half sibs is avoided in the alternative families; 6, family continuous generation breeding: a reciprocal hybridization method among families is adopted and an excellent sipunculus nudus variety with the high growth speed is bred. By adopting the method disclosed by the invention, the problem about healthy and scientific breeding of the good variety population of sipunculus nudus is solved.

The invention discloses a granular compound feed for the intermediate culture of sipunculus nudus. The feed comprises the following ingredients by weight percent: 32-40% of white fish meal, 5-11% of bean pulp, 4-8% of peanut meal, 6-10% of sargassum powder, 4-8% of shrimp shell meal, 2-5% of squid liver powder, 2-5% of beer yeast, 18-22% of flour, 2-4% of zeolite powder, 2-4% of fish oil, 0.5-2% of soya bean lecithin, 0.5-1% of composite mineral salt, 0.5-1% of composite vitamin and 0.05-0.08% of ethoxyquin. The nutritional ingredients in the feed disclosed by the invention can meet fast growth demand of the sipunculus nudus in an intermediate culture stage; the feed is good in stability in water, low in dissolution rate, small in pollution on a water body, good in palatability, and high in conversion rate; the particle size of the feed is suitable for the mouth diameter of the sipunculus nudus in the intermediate culture stage; the raw materials are reliable in resources and low in cost; and the preparation process is simple. When the feed disclosed by the invention is applied to feeding the sipunculus nudus in the intermediate culture stage, survival rate is high, growth speed is fast, the intermediate culture time of the sipunculus nudus is shortened, and the artificial breeding efficiency and the breeding production efficiency of the sipunculus nudus are improved.

The invention discloses health maintenance paste. The health maintenance paste is prepared from 25-35 parts of white mushrooms, 22-28 parts of calcined oysters, 22-26 parts of sipunculus nudus, 20-25 parts of sweet almonds, 15-22 parts of walnut peel, 12-18 parts of sesame, 10-15 parts of dried bean curd, 10-12 parts of elderberries, 8-10 parts of corn stigma and 20-25 parts of honey. According to the health maintenance paste, through the raw material combination of the traditional Chinese medicine, the purposes of tonifying Qi, strengthening the kidney, improving sleep, securing essence and cultivating Yuan, nourishing and protecting the liver, preventing cardiovascular and cerebrovascular diseases, lowering the blood pressure, preventing diabetes mellitus and enhancing the immune function of a human body are achieved; by reasonably determining the using ratio of the raw materials, a certain synergistic effect among the raw materials is generated, and therefore the product effect is optimized.

The invention discloses a processing method of dried sand-free sipunculus nudus. The processing method comprises following steps: step (1), turning washing: fresh and alive sipunculus nudus with sand on the body surface washed off is taken and is poked to overturn from the tail to rhynchodaeum by a blunt-end bamboo stick or steel needle which is 10-20 cm long, viscera are removed until no residues are left, and the sipunculus nudus is cleaned; step (2), sand removing: the rhynchodaeum of the sipunculus nudus in the step (1) is cut off by a pair of scissors, ventral nerve cords embedded in longitudinal lines of the body wall are pulled open, an ultrasonic cleaner performs ultrasonic cleaning for 1-3 min, an electric mixer performs mixing under 10-15 rpm during ultrasonic cleaning, and accordingly, sand grains hidden in the lines and gaps of the body wall of the sipunculus nudus are sunk to the bottom; step (3), blanching: the sipunculus nudus subjected to sand removing treatment in the step (2) is put in hot water at the temperature of 70-100 DEG C and blanched for 30-90 s, and the sipunculus nudus is continuously stirred for uniform heating during blanching and then is fished out and drained; step (4), drying: the sipunculus nudus drained in the step (3) is arranged in a mesh, put in a drying oven at the temperature of 45-55 DEG C and subjected to blast drying until the moisture content is 8.96%-11.87%. The sand content of the dried sand-free sipunculus nudus is lower than 0.1%, the taste is fine and smooth, and a sand feeling is avoided.

The invention relates to the technical field of capturing device, and relates to a sipunculus nudus capturing device and an implementation method thereof. The capturing device includes a rack; a capturing mechanism, a walking mechanism and a driving mechanism are arranged on the rack. The capturing mechanism is located behind the walking direction of the capturing device and is connected with therack movably; the walking mechanism is used for realizing the walking operation of the capturing device; the driving mechanism is used for driving the walking mechanism and the capturing mechanism. The capturing mechanism includes a spray device and a capturing frame. The spray device is located in the opening direction of the capturing frame and is close to the bottom. The capturing frame is located behind the device walking direction and can go up and down. The spray device flushes sipunculus nudus out of soil, and in the walking process, the sipunculus nudus is captured and collected by thecapturing frame in the rear. The sipunculus nudus in beaches or seawater is captured in batch, capturing does not need to wait for falling tide of seawater before digging and capturing, the traditional way of capturing sipunculus nudus is changed, the capturing automation is achieved, and the efficiency and output of capturing are improved.

The invention relates to the technical field of medicines, in particular to a preparation method and application of sipunculus nudus polysaccharide. The method comprises the following steps: processing sipunculus nudus to extract polysaccharide, and mixing the polysaccharide with a sipunculus nudus extract, turmeric powder, sodium bicarbonate, citric acid and maltodextrin to prepare effervescent tablets. The new application of sipunculus nudus polysaccharide is explored, the sipunculus nudus polysaccharide is prepared into effervescent tablets convenient to carry and eat, the additional valueof sipunculus nudus is increased, and the preparation method is simple in process and suitable for large-scale production.

The invention relates to the technical field of food, and concretely relates to a sipunculus nudus peanut butter and a production method thereof. The sipunculus nudus peanut butter comprises the following raw materials: 50-55 parts of sipunculus nudus, 20-25 parts of peanut, 10-12 parts of dried orange peel, 6-8 parts of Cinnamomum camphora leaves, 15-18 parts of paprika, 9-13 parts of white granulated sugar, 6-8 parts of ginger, 8-10 parts of garlic clove, 6-9 parts of peanut oil, 13-16 parts of yeast, and 11-15 parts of salt. The raw materials are produced according to certain ratio and process program to produce the sipunculus nudus peanut butter. The sipunculus nudus peanut butter has the beneficial effect that the peanut contains abundant protein, fat, carbohydrate and vitamin A which are capable of promoting brain cell growth and enhancing memory, sipunculus nudus contains protein, polypeptide, and amino acid required by human body which have the functions of adjusting immunization and delaying aging, the sipunculus nudus peanut butter produced by peanut and sipunculus nudus has good mouthfeel, and contains the various nutrition components required by human body.

The invention discloses bait for Sipunculus nudus industrial aquaculture and a production method thereof. The bait comprises, by mass, 10-35% of plant algae powder, 15-25% of fish meal, 2-15% of shell powder and the balance soil. The production method of the bait comprises the following steps of crushing dried plant algae, shell and soil, adding fish meal into the plant algae, shell and soil powder, and packaging the mixed powder to obtain a finished product. The plant algae powder has the particle sizes of 30-80 meshes. The fish meal and shell powder have the particle sizes of 30-100 meshes. The shell powder has the particle sizes of 40-200 meshes. The soil powder has the particle sizes of 40-100 meshes. The production method realizes intensive industrial high-yield aquaculture of Sipunculus nudus and provides a favorable condition for Sipunculus nudus industrial aquaculture.