5 results about "Sipunculus nudus" patented technology

The invention relates to the technical field of bioactive peptides, in particular to application of sipunculus nudus polypeptide in preparation of a neuroprotective drug. According to the specific technical scheme, firstly, sipunculus nudus oligopeptide is obtained through enzymolysis and an ultrafiltration membrane separation technology, the sipunculus nudus oligopeptide is separated and purified through sephadex chromatography, and on the basis of a high-glucose-induced mouse hippocampal neuron HT22 cell damage model, the collected components are separated and purified to obtain the sipunculus nudus oligopeptide. The neuroprotective effects of the sipunculus nudus oligopeptide and the separated components thereof are deeply discussed, and then the components in the sipunculus nudus oligopeptide are further separated and purified and subjected to mass spectrum peptide sequence identification by adopting reverse-phase high performance liquid chromatography. Experimental results show that the three components separated from the sipunculus nudus oligopeptide through sephadex chromatography can effectively improve the cell activity of HT22 cells after high glucose damage, and accumulation of ROS (reactive oxygen species) in the cells and occurrence of apoptosis are remarkably reduced.

The invention relates to the technical field of peptide extraction, in particular to sipunculus nudus peptide and an extraction method and application thereof. According to the specific technical scheme, the method comprises the following steps: 1) crushing a sipunculus nudus raw material, adding water, adding sodium hydrogen sulfite and ascorbic acid, and stirring and soaking for 10-15 minutes; 2) grinding the soaked raw materials, and adjusting the pH value of the slurry to be alkaline; (3) adding alkali enzyme into the slurry, carrying out microwave enzymolysis for 45-50 minutes, adding papaya peptidase B, continuously carrying out microwave enzymolysis for 45-50 minutes, heating, inactivating, cooling, carrying out centrifugal filtration, and collecting filtrate; and 4) sequentially carrying out microfiltration, ultrafiltration and sodium filtration on the obtained filtrate to obtain the product. Microwave radiation is adopted for assisting enzymolysis, energy can be provided for an enzymolysis system, the temperature needed by enzymolysis can be maintained, the enzymolysis efficiency is greatly improved, the prepared sipunculus nudus peptide can stabilize iron ions, a safe and efficient iron supplementing preparation can be developed, meanwhile, reproductive function decline caused by obesity can be comprehensively repaired, the advantage of multi-dimensional conditioning is achieved, and the sipunculus nudus peptide has a good application prospect. And the method is suitable for metabolism-related reproductive disorder treatment.

This invention provides the application of exosomes derived from Sipunculus fangka in the preparation of anti-bone tumor drugs, relating to the field of biomedical technology. This invention verifies the effects of exosomes derived from Sipunculus fangka in vitro, finding that exosomes derived from Sipunculus fangka can inhibit the cell viability and proliferation of 143B cells, and can significantly inhibit the migration and invasion of 143B cells, exhibiting good anti-bone tumor effects and can be applied to the preparation of anti-bone tumor drugs.

The invention belongs to the technical field of medicines, and particularly relates to a composition for preventing and treating children obesity and a preparation method thereof. The composition comprises the following raw materials in parts by weight: 35-45 parts of active pharmaceutical ingredients and 8-15 parts of auxiliary materials, the active pharmaceutical ingredient is composed of sipunculus nudus polypeptide and catalpa glycoside in a mass ratio of 1: (1.5-3). The composition can inhibit the weight gain of mice with high fat diet, reduce the total cholesterol, triglyceride and low-density lipoprotein level in serum of the mice, and improve the high-density lipoprotein level in the serum. In addition, the composition can also improve liver fat accumulation and relieve fatty liver lesion caused by liver fat accumulation, and has no influence on renal functions.

This invention discloses the application of an oligopeptide derived from *Sipunculus nudus* and 5-FU in the preparation of drugs for treating colorectal cancer, belonging to the field of biomedical technology. This invention isolates an oligopeptide from *Sipunculus nudus*. Experimental verification shows that this small-molecule oligopeptide, when combined with 5-FU at high concentrations, significantly inhibits the proliferation of colon cancer cells, exhibiting a dose-dependent synergistic effect. Oligopeptide 659 and 5-FU synergistically activate the mitochondrial apoptosis pathway; combined administration enhances γ-H2AX focal point formation and exacerbates DNA double-strand breaks, thereby synergistically inhibiting CRC cell proliferation. In vivo analysis of the antitumor effect of peptide 659 using NOD-SCID female mice inoculated with DLD-1 cells further demonstrates the anti-colorectal tumor effect of oligopeptide 659. Analysis of mouse autopsies showed that, compared to the control group, oligopeptide 659 inhibited tumor growth in mice.