Preparation method and use of antioxidant sipunculus nudus extract

A technique of extracting A. trachomatis, applied in the field of preparation of anti-oxidant extracts of A. tracheida, which can solve the problem that there are no application reports of antioxidant components in the water layer of A. tracheida and no disclosure of extraction methods for anti-oxidant components. and other problems, to achieve the effect of strong anti-oxidation and simple process

Active Publication Date: 2017-05-17
广西海洋研究所有限责任公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

But unfortunately, in the prior art, there is no disclosure for the extraction method of the antioxidant components in the water layer of the square starworm, and there is no application report of the antioxidant components in the water layer of the square starworm, the meaning of the present invention is just It is to find an extraction method of the anti-oxidation water layer of the square starworm and the application of the extract

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  • Preparation method and use of antioxidant sipunculus nudus extract
  • Preparation method and use of antioxidant sipunculus nudus extract
  • Preparation method and use of antioxidant sipunculus nudus extract

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Preparation of the antioxidant extract of square starworm:

[0016] (1) Primary processing of the square star worm

[0017] Take 29 catties of fresh and alive S. nudus, wash it, turn over its body wall, dry the body wall in an oven at 70°C to obtain dry S. nudus with a water content of 20%, and crush it to a particle size of 0.5 Add 3 kg of 95% ethanol to soak the coarse grains of centimeters, and set aside; collect 2 kg of the coelom fluid of the phalaenopsis, add 3 kg of 95% ethanol to soak, mix well and set aside;

[0018] (2) Preparation of Ethanol extract

[0019] At room temperature, use the method of 40KHz ultrasonic extraction to extract the coarse grains and body cavity fluid of the starworm, each time for 0.5-1h, and extract 3-6 times in total; take the ethanol extract of the starworm and filter it twice with ultra-fast filter paper , and then use a rotary evaporator and an evaporation workstation to concentrate the filtrate under reduced pressure. The filtra...

Embodiment 2

[0023] (1) Detection of the reducing power of the anti-oxidant extract of the square gridworm:

[0024] Take 0.5mL samples of different concentrations, add 1.25mL PBS (0.2mol / L, PH=6.6) and K 3 Fe(CN) 6 (1%, g / mL), mix well, react at 50°C for 20min, then cool rapidly, add 1.25mL of 10% trichloroacetic acid, mix well, centrifuge at 3000r / min for 10min, take 5.0mL supernatant, add distilled water 4.0 mL, and 1 mL of FeCl3 (0.1%, m / v) solution, mix well, and measure the absorbance at 700 nm in a 1 cm cuvette after 10 min. With VC as the positive control, each measurement object was measured 3 times in parallel, and the average value was taken.

[0025] (2) The scavenging effect of DPPH·(1,1-diphenyl-2-trinitrophenylhydrazine) by the water layer of the square lattice insect:

[0026] Take 2mL of samples with different concentrations, add 2mL DPPH ethanol solution (0.14mmol / L), mix well, and react in the dark at room temperature for 20min, and measure the absorbance A at 517nm ...

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Abstract

The invention relates to a preparation method and use of antioxidant sipunculus nudus extract. The method comprises the following steps: performing extraction by adopting ethanol, performing ethyl acetate and water two-phase extraction, performing concentration under reduced pressure to obtain antioxidant active extract, and generating a free radical through simulating a chemical system to detect antioxidant activity. According to the preparation method and the use, an antioxidant active substance is prepared from sipunculus nudus living bodies and waste sipunculus nudus coelomic fluid, so that the added value of sipunculus nudus is increased, wastes are turned into wealth, and such an industry is green; a process is relatively simple, less in equipment investment and very suitable for large-scale production, and a foundation is laid for further development of a series of sipunculus nudus functional food; the prepared antioxidant active substance is applicable to the industries of food antioxidants, functional food and pharmaceuticals.

Description

technical field [0001] The invention relates to a preparation method and an application of an anti-oxidation extract of the phalaenopsis, and belongs to the technical field of extraction of marine active substances. Background technique [0002] The square star worm is a biological resource with important economic value in marine fishery resources. At present, the researches on the metabolism, physiology and ecological characteristics of D. gridia are mainly carried out in foreign countries, but there are few researches on the medicinal value of D. gridia. Domestically, researches on its reproductive biology and edible efficacy are mainly carried out. The research on its medicinal value is mainly based on the research on crude extracts, polysaccharides and peptides. The pharmacological activities mainly focus on anti-fatigue, anti-oxidation, improving immunity, Anti-radiation, anti-aging, anti-viral activity, liver-protecting effect, improving memory and so on. The anti-ox...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00A61K35/62A61P39/06
CPCA23V2002/00A61K35/62A23V2200/30A23V2250/2042
Inventor 徐艳张秀国邹杰彭慧静张琴杨家林
Owner 广西海洋研究所有限责任公司
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