96 results about "Viral Activity" patented technology

The invention belongs to the technical field of biological medicine, and particularly relates to application of anethomycin in preparation of medicine for inhibiting cysteine protease activity. The invention discovers that anethomycin has the function of inhibiting the activity of virus cysteine protease, especially 3C or 3CL protease, for the first time. Molecular docking proves that anethomycin can effectively bind to the catalytic activity center of Senecavirus (SVA) 3C protease. An in-vitro FRET enzyme activity experiment proves that the inhibition rate of 50 [mu] M of anethomycin on SVA 3C protease reaches up to 88.5%. Cellular level experiments show that the anethomycin can significantly inhibit replication of SVA viruses, shows broad-spectrum antiviral activity on various viruses such as encephalomyocarditis viruses (EMCV), porcine reproductive and respiratory syndrome viruses (PRRSV) and porcine deltacoronaviruses (PDCoV), and is low in cytotoxicity and high in selectivity index (SI).

The invention relates to the technical field of nitrogen heterocyclic compounds, in particular to nitrogen heterocyclic alkaloid and application thereof in preventing and treating plant viruses. The structural formula of the nitrogen heterocyclic alkaloid is shown in the specification, wherein X is selected from any one of-OCH3,-OCH2CH3 and-O (CH2) 2CH3. An antiviral activity test result shows that the azacyclo-alkaloid series compounds provided by the invention have antiviral activity, and the antiviral activity level of part of azacyclo-alkaloid reaches or even exceeds that of a positive control drug. Therefore, the azacyclo-alkaloid is expected to be applied to prevention and treatment of plant viruses.

The application discloses an indole 3-amide derivative, a preparation method and application thereof. The compound has a structure shown in general formula I. The application further relates to a pharmaceutical composition containing the compound with the structure of formula I. Activity screening experiments show that the compound has good anti-dengue virus activity, and thus the application further provides application of the compound in preparation of an anti-dengue virus drug.

The application relates to the technical field of nitrogen heterocyclic compounds, in particular to a nitrogen heterocyclic alkaloid and application thereof in preventing and treating plant viruses. The structural formula of the nitrogen heterocyclic alkaloid is shown in the description, X is selected from any one of -OCH3, -OCH2CH3 and -O(CH2)2CH3. The anti-virus activity test result shows that the nitrogen heterocyclic alkaloid series compounds provided by the application all have anti-virus activity, wherein the anti-virus activity level of part of the nitrogen heterocyclic alkaloids reaches or even exceeds that of a positive control drug. Therefore, the nitrogen heterocyclic alkaloid can be expected to be applied to the prevention and treatment of plant viruses.

The invention discloses an application of sodium oxamate in preparation of anti-influenza virus products, and provides an application of sodium oxamate as a lactic dehydrogenase A inhibitor in preparation of anti-influenza virus products, the sodium oxamate has broad-spectrum antiviral activity, and can play a unique action mechanism by regulating host cell metabolism (such as protein lactylation). And a new direction is provided for overcoming virus resistance.

This invention relates to a zebrafish model, its establishment method, and its applications, relating to the biomedical field. The establishment method includes the following steps: injecting zebrafish with polyinosinic-polycytidylic acid (poly I:C), resuscitating, and culturing. This method can obtain a stable zebrafish model that can simulate an inflammatory state similar to that in humans following viral infection. This model can be used to assess whether antiviral drugs inhibit viral activity and the differences in efficacy among different antiviral drugs, thereby enabling preliminary screening of antiviral drugs.

The invention relates to the technical field of gene engineering, in particular to a novel calreticulin-carrying oncolytic adenovirus as well as application and a preparation method thereof. In the oncolytic adenovirus, a plasmid skeleton of a recombinant plasmid is type-5 adenovirus, and a nucleotide sequence of an E3 region of the plasmid skeleton has Gp19k fragment deletion; the recombinant plasmid comprises a CMV promoter and a nucleotide sequence used for coding calreticulin, the CMV promoter and the nucleotide sequence used for coding calreticulin are located at the deletion position of a Gp19k fragment, and the CMV promoter and the nucleotide sequence used for coding calreticulin are introduced into the deletion position of the Gp19k fragment of the type 5 adenovirus. The oncolytic virus can be normally replicated, calreticulin overexpression is realized under the condition that the virus activity is ensured, and the method has a good effect on various cancer cells, and can become an effective means for a'general cancer species' therapy.

The invention discloses a nucleoside antiviral NHC diester prodrug as shown in a formula I and pharmaceutically acceptable salts thereof, and a preparation method and medical application of the nucleoside antiviral NHC diester prodrug. Pharmacological experiments prove that the compound disclosed by the invention has relatively strong inhibitory activity on replication of multiple RNA viruses. Particularly, the compound has good lung microsome release efficiency, the species difference of the lung release efficiency is small, the consistency of the in-vivo drug effect of the antiviral drug and the in-vivo drug effect of the antiviral drug can be improved, and the risk of species difference of prodrug release antiviral active ingredients is avoided. Therefore, the compound provided by the invention has good broad-spectrum antiviral activity, and can be used as a broad-spectrum antiviral drug for treating various respiratory virus infections.

The invention relates to application of a compound in preparation of a medicine for treating related diseases and / or symptoms caused by new coronavirus infection. The compound has efficient activity of inhibiting new coronavirus infection in vivo and in vitro, and is low in cytotoxicity. Meanwhile, as a host AXL receptor inhibitor, the SARS-CoV-2 inhibitor blocks the binding site of the SARS-CoV-2 and the receptor AXL of the SARS-CoV-2, and as an interferon inducer, the SARS-CoV-2 inhibitor can strongly up-regulate the expression of I-type interferon, and double-targeting host receptor and interferon pathway can exert strong anti-neocoronavirus activity. Therefore, the compound disclosed by the invention is based on a novel anti-new coronavirus double-targeting strategy of blocking AXL receptor effect and up-regulating interferon response, is relatively safe to host cells, and can be used for preventing and treating new coronavirus infection.

The invention discloses a compound as shown in a formula 1 and pharmaceutically acceptable salts thereof, and discloses a preparation method and application thereof. The compound as shown in the formula 1 in the figure 2 and the pharmaceutically acceptable salt thereof can be used for preparing antiviral drugs or drugs for treating dengue fever. The compound as shown in the formula 1 provided by the invention has good anti-dengue virus activity and low cytotoxicity, DENV1-4 can be remarkably inhibited at the concentration of 1 mu M, especially EC50 of cellular level anti-DENV-2 can reach 2.12 nM, and stronger antiviral activity is shown. In addition, the CC50 of the compound 1 is greater than 200 [mu] M, and the toxicity to host cells is lower. The compound 1 provided by the invention has higher safety and treatment window, has potential to be used for preparing anti-dengue virus drugs, and has great popularization and application values.

The invention relates to an anti-influenza compound as well as a preparation method and application thereof, and the anti-influenza compound has a structure as shown in formula (I): (I) wherein X1 is CR3 or N; x2 is CR4 or N; z is CR5 or N; y1 is CR6 or N; y2 is CR7 or N; y3 is CR8 or N; w is-NH-or-N = CH-; in the formula, R1 is-R < 9 >-COO < 10 >,-(CR < 122 >) < n >-R < 11 >-(CR < 122 >) < n >-O-H,-(CR < 122 >) < m >-O-H,-(CR < 122 >) < h >-COO < 10 > or-R < 11 >-CONR < 13 >-R < 14 >. The compound provided by the invention has strong inhibitory activity on influenza virus, and is subjected to in-vivo antiviral activity test, action mechanism analysis and pharmacokinetic characteristic evaluation at the same time.

The invention provides an application of Akti-1 / 2 in preparation of an anti-new coronavirus drug. And the new coronavirus is SARS-CoV-2 (Sarcabose Region The chemical name of the Akti-1 / 2 is 1, 3-dihydro-1-[1-[4-(6-phenyl-1H-imidazo [4, 5-g] quinoxaline-7-yl) phenylmethyl]-4-piperidinyl]-2H-benzimidazole-2-ketone, in-vitro Vero cell infection model research shows that the Akti-1 / 2 can inhibit replication of an original strain of the new coronavirus (SARS-CoV-2), and the activity of resisting the new coronavirus is achieved. In a Vero cell infection model, the IC50 (half maximal inhibitory concentration) of the SARS-CoV-2 original strain is 0.039 [mu] M. Therefore, the Akti-1 / 2 can be used for research and development of anti-new coronavirus infection drugs, and has a good development prospect.

An ink comprises (i) a carrier; (ii) graphene and / or graphene oxide particles dispersed in the carrier; and (iii) a viral active and / or anti-microbial component adhered to the graphene and / or graphene oxide particles.

This device for outputting a virus action suppressing component carries out: a step for detecting the humidity in a space when an active ingredient composed of a virus action suppressing component is outputted into the space; a step for determining whether the humidity in the space is at least a first set value; and a step, if the humidity in the space is at least the first set value, for increasing the amount of the active ingredient outputted into the space more than in the case where the humidity in the space is less than the first set value. This configuration makes it possible to increase the virus action suppressing effect when the humidity is high.

The invention discloses a heterocyclic compound shown as a formula (I) or a formula (IIa) or a stereoisomer and a medicinal salt thereof, and a pharmaceutical composition containing the formula (I) or the formula (IIa). The compound disclosed by the invention has remarkable anti-HSV virus activity and physicochemical properties of a long-acting compound, and can be used for preparing a drug of a virus HSV inhibitor or a pharmaceutical composition containing the drug and the like.

The invention discloses a nucleoside compound and application thereof, and mainly relates to a nucleoside compound as shown in a formula (I) or various prodrugs, stereoisomers, pharmaceutically acceptable salts and racemic compounds of the nucleoside compound, a pharmaceutical composition containing the formula (I) and application of the nucleoside compound or the prodrugs, the stereoisomers, the pharmaceutically acceptable salts and the racemic compounds in preparation of drugs for preventing and / or treating RNA virus infection. Furthermore, the compound disclosed by the invention has remarkable broad-spectrum anti-RNA virus activity, overcomes the instability of a raw medicine in water, has a good patent medicine prospect and good oral bioavailability, and can be used for preparing a medicine of a virus RNA dependent RNA polymerase inhibitor or a medicine composition containing the medicine and the like.

Described herein are compositions, uses thereof, and methods for treating a viral infection in a host cell or organism infected by the virus, such as coronaviruses (e.g., severe acute respiratory syndrome coronavirus [SARS-CoV], severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2, the virus and its mutant forms that cause COVID-19], Middle East respiratory syndrome coronavirus [MERS-CoV]), Zika virus, Lassa virus, Crimean Congo hemorrhagic fever virus, hepatitis E virus, and other RNA viruses. Also described herein are synthetic rocaglate compositions, uses thereof, and methods for reducing or inhibiting translation initiation of a messenger ribonucleic acid (mRNA) of a virus in a host cell or organism infected by the virus.

Described herein are compositions, uses thereof, and methods for treating a viral infection in a host cell or organism infected by the virus, such as coronaviruses, Zika virus, Lassa virus, Crimean Congo hemorrhagic fever virus, hepatitis E virus, and other RNA viruses. Also described herein are synthetic rocaglate compositions, uses thereof, and methods for reducing or inhibiting translation initiation of a messenger ribonucleic acid (mRNA) of a virus in a host cell or organism infected by the virus.

The invention relates to the technical field of biological medicine, in particular to application of geldanamycin in preparation of a medicine for preventing and treating Newcastle disease virus. The compound with antifungal activity is obtained by separating, purifying and identifying the active substances of the strain, and is further verified to be geldanamycin. Through intracellular and extracellular combined experiment verification, the specific antiviral activity of the drug geldanamycin on the Newcastle disease virus is defined, and the geldanamycin can inhibit protein expression of the Newcastle disease virus, inhibit virions from being adsorbed to cells and directly inactivate the Newcastle disease virions; meanwhile, the geldanamycin can also up-regulate expression of an antiviral interferon gene in a cell, so that an all-around antiviral effect is realized.

The embodiment of the invention discloses a method and a kit for rapidly and accurately quantifying an active lentiviral vector. The method comprises the following steps: treating a lentiviral vector sample by using a treating fluid to obtain a pretreated sample; carrying out first fluorescence labeling on the sample by using specific dye labeled lentiviral vector nucleic acid; performing second fluorescence labeling on the sample by using a specific protein antibody or aptamer; detecting fluorescence signals of the particles in the sample by using a flow cytometer; and counting the number of the particles jointly marked by the first fluorescence and the second fluorescence in the sample so as to calculate the number of the active lentiviral vectors. According to the quantitative method for the activity of the lentiviral vector, accurate qualitative and quantitative detection is carried out on the active lentiviral vector; the method can distinguish the complete lentiviral vector from other empty-shell viruses, free nucleic acids and the like, has the characteristics of accurate virus activity characterization and short measurement time, and can complete all detection within 1 hour.

The invention discloses a phoebe zhennan water-soluble extract as well as an extraction method and application thereof, and belongs to the technical field of medicine extraction. The method comprises the following steps: S1, crushing a phoebe zhennan raw material, adding 6 times of water, extracting twice, 1 hour each time, and combining filtrates; s2, concentrating the filtrate obtained in the step S1 under reduced pressure until the volume is 1: 1, standing at low temperature, and taking supernate; and S3, centrifuging the supernate obtained in S2, discarding the precipitate, concentrating under reduced pressure, and drying at low temperature to obtain the phoebe zhennan water-soluble extract. The invention discloses a phoebe zhennan water-soluble extract and an extraction method and application thereof. The phoebe zhennan water-soluble extract has efficient broad-spectrum antiviral activity and can effectively inhibit respiratory virus activity.

The invention discloses an application of an eIF2alpha kinase regulator in preparation of an anti-human coronavirus drug. Specifically, the compound provided by the invention has the activity of resisting human coronavirus HCoV-OC43 and / or resisting new coronavirus of human coronavirus. The invention focuses on antiviral host factors, provides a target compound for targeting host eIF2alpha kinase, eIF2alpha phosphorylation and protein synthesis, plays an antiviral role by accurately regulating and controlling host virus interaction, and is beneficial to overcoming virus resistance and obtaining broad-spectrum antiviral activity.

The invention relates to the technical field of medicines, and particularly discloses application of interferon alpha combined with stiripentanol in virus infection resistance. As an antiviral drug, interferon alpha has the problems of limited curative effect, remarkable side effect, narrow applicable crowd, inconvenience in medication and the like clinically. The invention finds that the accumulation of host lactic acid in the later stage of virus infection is a key negative factor, and lactic acid directly inhibits the antiviral activity of interferon alpha and induces inflammatory storm. Through combined use of a lactic dehydrogenase inhibitor stiripentol (Stiripentol, STP), lactic acid production is reduced, a lactic acid-mediated antiviral inhibition-inflammation promotion effect is reversed, and the antiviral activity of interferon alpha is enhanced and side effects are reduced. Experiments prove that the combined therapy can effectively inhibit virus replication and inflammatory factor expression, and a new strategy is provided for broad-spectrum antiviral therapy.

The invention discloses a preparation based on bug defensin as well as a preparation method and application thereof. The prepared porous fiber serves as a carrier, the high-temperature resistance of the bug defensin is improved, the porous fiber and phospholipid-chitosan-sodium alginate form dual protection, the activity of the bug defensin can be effectively guaranteed, the certain high-temperature-resistant activity is achieved, and the excellent feed processing adaptability is achieved. Besides, agricultural waste is adopted to prepare porous fibers, biocompatibility is higher, the porous fibers are loaded and then wrapped, the structure is not prone to being damaged, bad smells of the porous fibers can be covered, the food consumption is increased, immune regulation is participated, growth of animals is effectively promoted, on the whole, the mode of combining loading and wrapping is designed, the process is simple, and the cost is low. The entrapment efficiency is high, the antibacterial and antiviral activity is exerted, meanwhile, drug resistance is not prone to being generated, and the safety performance is high.

Disclosed in the present invention are an anti-SARS-CoV-2 arylnaphthalene lignan compound, and a preparation method therefor and the use thereof. Results show that ANL-1, ANL-2, and ANL-4 to ANL-7 have no toxicity to Vero E6 cells, and ANL-3 has low toxicity to Vero E6 cells. In a Vero E6 cell model, the in vitro anti-SARS-CoV-2 activities of ANL-7 and ANL-2, in TI value, are respectively 19 times and 16 times that of remdesivir, and are higher than the in-vitro anti-SARS-CoV-2 activity of a market-available drug. ANL-2 has a significant inhibitory effect on RNA-dependent RdRp activity of SARS-CoV-2, and ANL-2 also effectively limits the replication of various pathogenic coronaviruses in Caco2 cells or hamsters. It can be seen that the arylnaphthalene lignan compound has a good anti-coronavirus activity and can be used for treating and preventing SARS-CoV-2 infection or delaying the progress of SARS-CoV-2 infection in a patient.

The invention discloses lactobacillus mucosa with porcine virus resisting activity and application of the lactobacillus mucosa, and relates to the technical field of microorganism application and virus prevention and control. It is found for the first time that the lactobacillus mucosa has broad-spectrum antiviral activity, not only has a remarkable inhibition effect on porcine reproductive and respiratory syndrome virus type 2 (PRRSV2) strains, but also can further resist porcine epidemic diarrhea virus (PEDV) and porcine rotavirus (PoRV); according to the present invention, the Lactobacillus mucosa is adopted to culture the supernatant, such that the supernatant does not have toxicity on Marc-145 cells; according to the PRRSV2 strain treated by using the supernatant, the expression level of virus RNA and protein and the filial generation virus titer are remarkably reduced, so that the infection ability is weakened; the culture supernatant directly acts with virus particles to block infection and replication of the virus particles; the product disclosed by the invention is derived from natural probiotics, is high in safety, environment-friendly and not easy to resist drugs, can be used for preparing a broad-spectrum anti-porcine virus biological preparation, provides a new strategy for preventing and controlling porcine virus diseases, and has a good industrial application prospect.

The invention discloses a nucleoside analogue as shown in a formula I and pharmaceutically acceptable salts thereof, and a preparation method and medical application of the nucleoside analogue and the pharmaceutically acceptable salts thereof, pharmacological experiments prove that the nucleoside analogue and the pharmaceutically acceptable salts thereof have relatively good inhibitory activity on RNA-dependent RNA polymerase, and can inhibit replication of various RNA viruses in the aspect of inhibiting virus replication, so that the nucleoside analogue and the pharmaceutically acceptable salts thereof have good application prospects. Particularly, the compound has relatively good biological activity for treating influenza virus and vesicular stomatitis virus infection. Therefore, the compound provided by the invention has good broad-spectrum antiviral activity, can be used as a broad-spectrum antiviral drug to treat various virus infections, and enriches the inventory of antiviral drugs. The nucleoside analogue has anti-RNA (Ribonucleic Acid) virus activity.

The application discloses an azabicyclo[3.1.0]hexane derivative and a preparation method and application thereof, and belongs to the field of biological medicines. The application provides a compound shown in the following formula or a stereoisomer, a geometric isomer, a tautomer, a nitroxide, a hydrate, a solvate, a pharmaceutically acceptable salt or a prodrug thereof; wherein R1 is selected from an alkyl group, an alkylamine group and an alkoxy group, R2 is selected from a substituted or unsubstituted alkyl group, a cycloalkyl group, an aryl group and a heterocyclic alkyl group, and R3 is selected from -CHO or -CH(OH)SO3Na. The compound has high anti-novel coronavirus activity, can effectively treat and prevent novel coronavirus pneumonia, has the advantage of being orally taken, and provides a new idea for developing orally taken anti-novel coronavirus drugs.

The present application relates to the technical field of biological medicine, in particular to a preparation method of varicella attenuated live vaccine, a freeze-drying protective agent and application thereof, which comprises the steps of cell culture, virus infection, complex enzymatic dissociation, protective ultrasonic disruption, clarification and collection, etc. The complex enzymatic dissociation adopts EDTA and recombinant trypsin combination to loosen the cells at low temperature; the protective ultrasonic disruption adopts a buffer solution containing trehalose and recombinant human blood albumin to resuspend the cells and perform intermittent ultrasonic disruption. The present application further provides a freeze-drying protective agent which is composed of trehalose, sorbitol, L-arginine, L-histidine and polyvinylpyrrolidone. The virus harvesting efficiency of the method is high, and the virus activity loss is small; the freeze-drying protective agent has good safety and excellent stability.

The invention discloses a preparation method and application of a monoclonal antibody based on recombinant goose circovirus Cap protein, and belongs to the technical field of biology. The monoclonal antibody prepared based on the recombinant goose circovirus Cap protein has excellent specificity and affinity, has excellent reactivity with the goose circovirus, and also has high specificity and sensitivity when being applied to preparation of diagnostic reagents. Besides, the prepared monoclonal antibody is applied to non-therapeutic purpose neutralization virus activity and passive immune activity, and the monoclonal body can block the infection mechanism by combining virus key protein (Cap protein); the antibody targets the neutralizing epitope of the virus capsid protein and inhibits virus replication; instant immune protection can be provided by directly injecting the monoclonal antibody, interaction with virus protein can be achieved, neutralizing epitopes can be accurately positioned, vaccine design can be guided, the monoclonal antibody can be used for quantitative detection of the antibody level after vaccine immunization, and the monoclonal antibody has important significance in virus diagnosis and illness state evaluation.