13530results about "Antinoxious agents" patented technology

The invention relates to a high-activity recombinant XVII type collagen as well as a rapid preparation method and application thereof, and belongs to the technical field of bioengineering. The amino acid sequence of the high-activity recombinant XVII type collagen is as shown in SEQ ID NO. 1, and the nucleotide sequence of the high-activity recombinant XVII type collagen is as shown in SEQ ID NO. 2. The invention also provides a preparation method and application of the recombinant XVII type collagen. The high-activity recombinant XVII type collagen has good biological activity, has good effects of promoting cell adhesion, cell proliferation and cell migration, and can be compounded with other proteins to form raw materials of tissue engineering products, cosmetics, health care products or medicines. The high-activity recombinant XVII type collagen gene is integrated to different positions of a yeast genome through three different vectors, and the copy number of a target gene is increased, so that the expression quantity of the recombinant XVII type collagen is remarkably increased, and the yield of the high-activity recombinant XVII type collagen is effectively increased.

The invention provides isolated Ackermansiella muciniphila, a composition containing the same and an anti-aging and / or anti-oxidation application of the Ackermansiella muciniphila. The Akkermansia muciniphila has the advantages that the average life and the longest life of the Akkermansia muciniphila in a nematode model can be prolonged, the movement activity, the swallowing function and the reproductive capacity of the Akkermansia muciniphila can be improved, the expression of insulin / IGF-1 pathway genes (daf-16, daf-2 and age-1), the expression of antioxidant genes (gst-4 and sod-3) and the expression of longevity related genes (sir-2.1) of the Akkermansia muciniphila can be up-regulated, and the antioxidant capacity and the heat stress tolerance of the Akkermansia muciniphila can be enhanced. It is shown that the Akkermansia muciniphila provided by the invention can be used for preparing or developing the anti-oxidation and anti-aging products, and the anti-oxidation and anti-aging products can be prepared from the Akkermansia muciniphila. The invention provides an efficient and safe solution for anti-aging and / or anti-oxidation.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a plant lactobacillus XD117, application of the plant lactobacillus XD117 in fermentation of astragalus membranaceus, an astragalus membranaceus fermentation product and a preparation method of the astragalus membranaceus fermentation product. The bacterial strain is lactobacillus plantarum XD117 and is preserved in the China Center for Type Culture Collection, and the preservation number of the bacterial strain is CCTCC NO: M 20241730. The preparation method of the astragalus mongholicus fermentation product comprises the following steps: inoculating the lactobacillus plantarum XD117 into astragalus mongholicus enzymatic hydrolysate, and fermenting in a fermentation temperature range suitable for the lactobacillus plantarum XD117 to obtain the astragalus mongholicus fermentation product. The total content of calycosin, formononetin, calycosin glucoside and formononin in the astragalus mongholicus fermentation product is high, and the astragalus mongholicus fermentation product has a very good antioxidant effect.

The invention discloses red ginseng fermentation liquor with high rare saponin content and functional bioactivity and a preparation method thereof, and the preparation method comprises the following steps: mixing red ginseng powder with water to obtain red ginseng mixed liquor; cellulase is added into the red ginseng mixed solution for enzymolysis, and red ginseng enzymatic hydrolysate is obtained; or heating the red ginseng liquid, and performing hot water extraction to obtain a red ginseng extracting solution; heating the red ginseng extracting solution to be boiled, and after sterilization is finished, adding lactic acid bacteria powder for fermentation after the red ginseng solution is cooled, so as to obtain red ginseng fermentation liquor; and carrying out solid-liquid separation on the fermentation broth to obtain a fermentation supernatant, and sterilizing the red ginseng fermentation broth to obtain the sterilized red ginseng fermentation broth. According to the preparation method disclosed by the invention, the content of total saponins in the red ginseng fermentation liquor is increased, the variety and content of rare saponins can be increased, and in addition, the prepared red ginseng fermentation liquor has relatively high antioxidant activity, tyrosinase inhibition rate activity, leukoproteinase inhibition activity and hyaluronidase inhibition activity; wide application prospects and market potentials are realized.

The invention provides a preparation method of a polygonatum sibiricum fermentation product, which comprises the following steps: A) pretreating a polygonatum sibiricum raw material, and mixing with water to obtain polygonatum sibiricum slurry; b) heating the polygonatum sibiricum slurry, mixing the heated polygonatum sibiricum slurry with a nitrogen source and a phosphorus source, and performing primary sterilization to obtain sterilized polygonatum sibiricum slurry; c) inoculating the sterilized rhizoma polygonati slurry with saccharomycetes, and fermenting to obtain fermentation liquor; d) adding a carbon source into the fermentation liquor, performing secondary sterilization, inoculating lactic acid bacteria, performing secondary fermentation, and performing third sterilization to obtain secondary fermentation liquor; and E) adding a compound enzyme into the secondary fermentation liquid for enzymolysis, then sterilizing for four times, and centrifuging to obtain the polygonatum sibiricum fermentation product. 3000 Da), a small molecule polypeptide (lt; the polygonatum kingianum fermentation product prepared by the method disclosed by the invention has the advantages that the conversion of polygonatum kingianum saponin can be promoted, the contents of diosgenin and trilliin are increased and calibrated, and the polygonatum kingianum fermentation product has the effects of improving the reaction capability and the exercise capability.

The invention discloses lactobacillus kefiranofaciens IMAU12319 and application thereof, belongs to the technical field of microorganisms, and particularly relates to lactobacillus kefiranofaciens IMAU12319 which is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 8, 2024, the preservation address is No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, and the number is CGMCC No.31224. The lactobacillus kefiranofaciens IMAU12319 has the advantages that the lactobacillus kefiranofaciens IMAU12319 can be used for preparing the lactobacillus kefiranofaciens IMAU12319, and the lactobacillus kefiranofaciens IMAU12319 can be used for preparing the lactobacillus kefiranofaciens IMAU12319; the invention also relates to an application of the lactobacillus kefiranofaciens IMAU12319 in preparation of a probiotic agent capable of resisting gastrointestinal digestion. The strain disclosed by the invention not only shows good gastrointestinal tract endurance capacity, but also shows relatively strong potential in the aspects of intestinal adhesion, oxidation resistance and bacteriostasis, and has a wide application prospect in leavening agent strains.

The invention relates to the technical field of biocatalysts, in particular to a FeCo-NC bimetallic monatomic nano-enzyme as well as a preparation method and application thereof. The preparation method of the nano enzyme comprises the following steps: preparing Co-ZIF-8 from zinc nitrate, cobalt nitrate and 2-methylimidazole; the Co-ZIF-8 and ferric chloride are used for preparing the FeCo-NC bimetallic monatomic nano-enzyme, the molar ratio of the zinc nitrate to the cobalt nitrate is (20-30): 1, and the molar ratio of the cobalt nitrate to the ferric chloride is (2-6): 1. The nano-enzyme prepared by the embodiment of the invention is of a monatomic structure, has double-enzyme activity, can simultaneously simulate catalase and NADH oxidase, and has the capability of removing active oxygen.

The invention discloses two brand-new bioactive peptides with antioxidant and alpha-glucosidase inhibitory activity in traditional Chinese medicine hippocampus. The sequences of the novel bioactive peptides provided by the invention are respectively YLPPNW and SVWLGGSLL. According to the method, purity and structure identification is carried out on the new bioactive peptide obtained through separation by adopting liquid phase, primary mass spectrum and secondary mass spectrum, and a material basis is provided for further quality control and drug effect research of the traditional Chinese medicine hippocampus.

The invention relates to a method for screening and preparing a horse milk whey protein source antioxidant peptide and application of the horse milk whey protein source antioxidant peptide. The polypeptide with potential antioxidant activity is screened out by predicting information of the polypeptide through virtual enzymolysis and a computer and then performing molecular docking with the keap1 protein. The feasibility of obtaining the antioxidant peptide is verified by simulating gastrointestinal digestion with pepsin, trypsin and chymotrypsin. Finally, in-vitro chemical antioxidant activity verification is carried out through chemical synthesis of the high-purity antioxidant peptide, and it is found that the antioxidant peptide has good free radical scavenging activity. An oxidative damage model is induced by H2O2 on cells, and it is found that the antioxidant peptide can resist cell oxidative damage by exciting the activity of a series of antioxidant enzymes. The natural antioxidant peptide screened by the invention has wide application prospects in the fields of foods, medicines, cosmetics and the like, and an efficient way is provided for development of horse milk whey protein source functional polypeptide products.

The invention relates to the technical field of microorganisms, in particular to plant lactobacillus MPs-68 capable of adsorbing microplastics and degrading uric acid and application of the plant lactobacillus MPs-68. The provided plant lactobacillus MPs-68 capable of adsorbing microplastics and degrading uric acid is a marine-derived natural strain, the preservation number is CGMCC No.31976, the plant lactobacillus MPs-68 has a remarkable adsorption effect on microplastics polystyrene and polyethylene, has the function of degrading uric acid, and is high in oxidation resistance, gastric acid resistance, cholate resistance and intestinal colonization capacity, and the plant lactobacillus MPs-68 can be used for adsorbing microplastics and degrading uric acid. The method has important application value in the fields of food or health care products for assisting adsorption of micro-plastics and reduction of uric acid and the like. The invention also provides a microbial agent, a probiotic product, a functional food, a fermentation product, a microecological preparation, a microplastic adsorbent and a microplastic adsorbent compounded with the lactobacillus brevis MPA01 (the preservation number is CGMCC No.31977) containing the phytobacterium plantarum MPs-68 capable of adsorbing the microplastics and degrading the uric acid, and the microplastic adsorbent has the effects of adsorbing the microplastics, degrading the uric acid and the like.

The preparation method comprises the following steps: mixing a tannic acid solution and a tetravalent cerium salt solution, adjusting the pH value to 9-11, and reacting to obtain the cerium tannate nano-enzyme, the molar ratio of the tannic acid to the tetravalent cerium salt is (1-4): 1; the hydration dynamic diameter of the cerium tannate nano-enzyme is 20 to 80 nm. The TA-Ce nano-enzyme prepared by the preparation method disclosed by the invention is smaller in particle size, can keep stable and good dispersibility in a physiological environment, and has good biocompatibility, low hemolysis rate and small systemic toxicity; meanwhile, the compound has good kidney targeting property, can be effectively enriched into the injured kidney, eliminates various ROS (reactive oxygen species) and relieves oxidative stress by utilizing unique redox cycle between Ce < 3 + > and Ce < 4 + > states, and has a good effect in treating acute kidney injury induced by rhabdomyolysis and / or cis-platinum. The research lays a foundation for the metal polyphenol nano material in the treatment of acute kidney injury.

The invention relates to the technical field of hippocampus extract, in particular to a hippocampus-sourced neuroprotection and anti-oxidative stress polypeptide and a preparation method thereof.The amino acid sequence of the polypeptide is shown as SEQ ID No.1. The preparation method comprises the following steps that 1, 500 g of fresh hippocampus is weighed, surface silt is removed, freeze-drying is conducted at the temperature of-60 DEG C, then the hippocampus is smashed through a smashing machine and sieved through a 20-mesh sieve, and the hippocampus extract is obtained; dialyzing with distilled water for 24 hours, and freeze-drying to obtain hippocampus powder; (2) weighing 200g of hippocampus powder, adding 1000ml of 95% ethanol, extracting for three times, removing fat-soluble substances, and extracting and freeze-drying precipitates by using a 1 mol / L NaCl solution under the condition of 4 DEG C in combination with ultrasonic assistance to obtain crude hippocampus protein; (3) the hippocampus crude protein is taken, the hippocampus polypeptide is prepared through an ultrasonic-assisted bionic digestion method, and the hippocampus polypeptide has the advantages of being specific in molecular structure, clear in action mechanism, stable in gastrointestinal tracts, capable of protecting nerves, resistant to oxidative stress and the like.

The invention discloses a nano-enzyme as well as a preparation method and application thereof, and relates to the technical field of nano-enzymes. According to the preparation method, natural polyphenol, a cerium source and polyethylene glycol are mixed to prepare the novel polyphenol nano-enzyme, the preparation method is simple and easy to implement, the preparation cost is low, the prepared nano-enzyme has good biocompatibility, and a new way is provided for effective treatment of inflammatory diseases.

The invention relates to the technical field of microorganisms, and in particular relates to application of a strain of lactobacillus mucus TG018. The invention further relates to a preparation method of the lactobacillus mucus TG018. The preservation number of the lactobacillus mucus TG018 is CCTCC (China Center for Type Culture Collection) No: M 2023514; the lactobacillus mucus TG018 can be used for producing nicotinic acid, serving as an antioxidant and / or preparing the antioxidant, or can be applied to medicines for reducing uric acid.

To provide a mitochondria function-improving agent or the like which improves a mitochondria function in a skin cell.SOLUTION: A mitochondria function-improving agent that improves a mitochondria function in a cell contains components included in a swallow's nest as an active ingredient. According to each point of view of the present invention, a mitochondria function-improving agent or the like which improves mitochondria function in a skin cell can be provided.SELECTED DRAWING: Figure 3

The invention relates to the technical field of nano material application. The invention provides a ruthenium-doped Prussian blue nano-enzyme and a preparation method thereof. The ruthenium-doped Prussian blue nano-enzyme is obtained by reacting metal ruthenium with Prussian blue nano-particles at normal temperature. The invention further provides a nano-enzyme library containing the ruthenium-doped Prussian blue nano-enzyme and a construction method of the nano-enzyme library. The invention also provides the SNP-PB nano-enzyme obtained by loading sodium nitroprusside on the ruthenium-doped Prussian blue nano-enzyme. The ruthenium-doped Prussian blue nano-enzyme and the SNP-PB nano-enzyme both have good antibacterial and anti-inflammatory performance, after NO gas release capacity is given, the problem of resistance of a biological membrane is better solved, then oxidative stress resistance and periodontal bacterial infection resistance are better exerted, and a new treatment method is provided for periodontal diseases.

The invention discloses multifunctional hydrogel and application thereof, and belongs to the technical field of biological medicine. According to the multifunctional hydrogel, ketal mercaptan with two hydrazide ends and oxidized dextran are cross-linked to form a cross-linked network containing dynamic covalent bonds. The preparation method comprises the following steps: dissolving ketal mercaptan with two hydrazide ends in deionized water to obtain a ketal mercaptan solution, dissolving oxidized dextran in deionized water to obtain an oxidized dextran solution, mixing the ketal mercaptan solution with the oxidized dextran solution, and standing to obtain the multifunctional hydrogel. The multifunctional hydrogel has pH / ROS dual responsiveness, can respond to inflammation microenvironment and low pH of infectious wounds, remove active oxygen, release therapeutic drugs, regulate macrophage polarization and relieve bacterial infection, thereby playing a key role in inflammation, proliferation and remodeling stages of wound healing to accelerate wound healing. The curcumin-encapsulated liposome is added into the multifunctional hydrogel, so that the antibacterial effect can be further improved.

The invention discloses a preparation method and application of fresh dendrobium officinale refined polysaccharide, and the preparation method of the fresh dendrobium officinale refined polysaccharide comprises the following steps: adding a proper amount of water into fresh dendrobium officinale, juicing, concentrating filtrate, adding ethanol for alcohol precipitation, dissolving precipitate with water, deproteinizing, and drying to obtain crude polysaccharide; the crude polysaccharide is subjected to graded alcohol precipitation and ion exchange column purification to obtain semi-refined polysaccharide, the semi-refined polysaccharide is purified by a gel column to obtain the fresh dendrobium officinale refined polysaccharide, and the polysaccharide is acidic glucomannan which is high in purity, uniform and moderate in molecular weight and contains beta-configuration pyranoside. In-vivo and in-vitro activity tests show that the compound polysaccharide has exact curative effects of preventing drunkenness, dispelling the effects of alcohol and preventing stomach injury and hepatocyte injury, is prepared by compounding the compound polysaccharide with traditional Chinese medicines of radix puerariae, hovenia dulcis thunb and tomato seed oil, and has the effect of obviously enhancing the immunity of liver functions.

The invention discloses a novel nano-drug Res (at) LDH and a preparation method and application thereof.Germanium-doped LDH powder and resveratrol are combined to obtain a nanoscale particle mixed material Res (at) LDH, electron paramagnetic resonance (EPR) tests show that both the germanium-doped LDH and the traditional drug resveratrol show excellent reactive oxygen species (ROS) removal characteristics, and the Res (at) LDH can be used for preparing a novel nano-drug Res (at) LDH. The nano-drug Res (at) LDH formed by combining the two has remarkable synergistic ROS removal efficiency which can reach 90%. Meanwhile, in-vivo biological distribution experiments show that the novel nano-drug Res (at) LDH has excellent blood-brain barrier permeability, enhances accumulation in brain tissues, improves neurological dysfunction, reduces infarct volume, shows strong antioxidant activity, and inhibits release of pro-inflammatory cytokines.

The invention relates to the technical field of biology, in particular to multifunctional belly-expanding hippocampal peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is utilized to prove the anti-oxidation effect and the effect of improving male reproductive disorder and testis injury of the multifunctional belly-expanding hippocampal peptide; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention belongs to the technical field of medicinal and edible Chinese herbal medicine alcoholic drink, and particularly relates to lactobacillus plantarum and application thereof in fermentation of ginsenoside. The lactobacillus plantarum is specifically lactobacillus plantarum L100, and the preservation number of the lactobacillus plantarum L100 is CGMCC (China General Microbiological Culture Collection Center) No. 34425. The lactobacillus plantarum L100 has the function of converting and preparing the rare ginsenoside, basic components of ginseng can be directionally converted into high-value rare ginsenoside (such as Rg3, Rg5 and Rh1), and particularly, the lactobacillus plantarum L100 has extremely high specificity on generation of F5 and Rh10. Ginseng fermentation liquor obtained by fermenting ginseng through lactobacillus plantarum L100 and extracting solutions of medicinal and edible components such as codonopsis pilosula, astragalus membranaceus, liquorice and cistanche deserticola are used for preparing the liqueur, and a liqueur product with potential effects in the aspects of oxidation resistance, inflammation resistance, immunoregulation and the like is obtained.

The invention relates to the field of preparation and application of biological peptides, in particular to a swelling hippocampal peptide and application of the swelling hippocampal peptide in preparation of anti-fatigue products. Five active peptide sequences with high biological activity are obtained through research, animal behavioral experiments prove that the belly distention hippocampal peptide has a remarkable anti-fatigue effect and can prolong the swimming exhaustion time and enhance the muscle strength, the anti-fatigue effect of the belly distention hippocampal peptide is verified, and a new way is provided for development of anti-fatigue hippocampal products.

The invention provides a preparation method of mitochondria-targeted antioxidant hybrid vesicles. The preparation method comprises the following steps: S1, preparing and separating adipose-derived stem cell nano-vesicles; s2, preparing a liposome loaded with dihydromyricetin and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide by an ethanol injection method; and S3, preparing the hybrid nano vesicles loaded with the dihydromyricetin and the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide. The invention also provides the hybrid vesicles prepared by the method and application of the hybrid vesicles in preparation of refractory diabetes wound treatment drugs. The lipidosome and the adipose-derived stem cell nano-vesicles are hybridized to prepare the hybridized vesicles capable of targeting mitochondria and resisting oxidation, the hybridized vesicles are applied to wound treatment for the first time, the problem of oxidative stress of diabetic wounds is solved, wound healing is accelerated, and a new strategy is provided for optimizing mitochondrial function defects and oxidative stress of the diabetic wounds.

The invention provides a metal polyphenol nanoparticle, a preparation method thereof and an application of the metal polyphenol nanoparticle in preparation of a medicine for treating acute lung injury, the metal polyphenol nanoparticle is formed by self-assembly of metal ions and polyphenol, the metal ions are selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >, and the polyphenol is selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >. The polyphenol is selected from epigallocatechin gallate (EGCG), caffeic acid (CA), chlorogenic acid (CGA), gallic acid (GA) and luteolin (LUT). The metal polyphenol nanoparticles are used for preparing a medicine for treating acute lung injury induced by sepsis, pneumonia, serious trauma or inhalation injury, and the medicine further comprises a pharmaceutically acceptable carrier. The metal polyphenol nanoparticles provided by the invention are simple in preparation process and high in biological safety, polyphenol and metal ions synergistically enhance the anti-inflammatory and antioxidant activity, and the metal polyphenol nanoparticles are superior to the single use of polyphenol or metal ions.

The invention provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of antioxidant and anti-aging products, and relates to the technical field of medicinal preparations. The preparation method comprises the following steps: (1) crushing; (2) extracting; (3) standing and separating; and (4) freezing and filtering to obtain the medicinal liquor. The medicinal liquor is obtained by extracting the traditional Chinese medicine components through the steps of crushing in batches, mixing and extracting, standing, freezing and separating the supernate and the suspension in different separation modes, and the medicinal liquor can better improve the space exploration capability of a mouse aged due to D-galactose, can reduce malondialdehyde MDA, and can improve the immunity of the mouse. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) is improved, and the obvious anti-oxidation and anti-aging effects are achieved.

The invention discloses a hydroxytyrosol-ergothioneine eutectic crystal as well as a preparation method and application thereof, and belongs to the technical fields of medicines, foods, daily chemicals and the like. The invention provides a hydroxytyrosol-ergothioneine eutectic crystal. The X-ray powder diffraction pattern of the hydroxytyrosol-ergothioneine eutectic crystal has characteristic diffraction peaks at least corresponding to the positions where the 2 theta value is 4.21 degrees + / -0.2 degrees, 8.36 degrees + / -0.2 degrees, 12.29 degrees + / -0.2 degrees, 15.29 degrees + / -0.2 degrees, 19.40 degrees + / -0.2 degrees, 20.98 degrees + / -0.2 degrees, 23.12 degrees + / -0.2 degrees and 28.73 degrees + / -0.2 degrees. According to the hydroxytyrosol-ergothioneine eutectic, the melting point of hydroxytyrosol is increased, the hygroscopicity of hydroxytyrosol is remarkably reduced, and meanwhile the stability of hydroxytyrosol and ergothioneine is enhanced. In addition, after the ergothioneine hydroxytyrosol forms the eutectic crystal, the ergothioneine hydroxytyrosol can also play better anti-oxidation and anti-aging effects.

The invention relates to Pediococcus pentosaceus PP90 for preventing and treating alcoholic nerve injury and application of the Pediococcus pentosaceus PP90, the Pediococcus pentosaceus PP90 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.32034. The Pediococcus pentosaceus PP90 can be used for preventing and treating alcoholic nerve injury. The fermentation product of the pediococcus pentosaceus PP90 can degrade the alcoholic strength of alcohol, can generate ethanol dehydrogenase and acetaldehyde dehydrogenase, can improve the activity of nerve cells, can regulate the expression of cytokines, can inhibit the activation of astrocytes, can regulate the expression of brain-derived neurotrophic factors, and can inhibit the growth of the brain-derived neurotrophic factors. The compound can improve the disordered neurotransmitter level, and can be used for preparing products for preventing or improving alcoholic nerve injury.

The invention discloses preparation and application of antioxidative, anti-inflammatory and hypoglycemic lactoferrin peptide, and belongs to the field of food science and nutriology. According to the invention, lactoferrin is used as a raw material, hydrolysis is carried out by using a composite enzymatic hydrolysis method, and the molecular weight is obtained by ultrafiltration; the preparation method comprises the following steps: preparing a component of antioxidant, anti-inflammatory and hypoglycemic peptide from the active peptide YLSW (Tyr-Leu-Ser-Trp), carrying out freeze drying on the component to obtain peptide powder rich in antioxidant, anti-inflammatory and hypoglycemic peptides, identifying peptide fragments contained in the peptide powder by utilizing an LC-MS / MS technology, and screening out the active peptide YLSW (Tyr-Leu-Ser-Trp) with antioxidant, anti-inflammatory and hypoglycemic triple functions. The active peptide is added into the product to obtain the antioxidant, anti-inflammatory and hypoglycemic functional product.

The invention relates to the technical field of pharmaceutical preparations, and discloses a composition with liver protection and antioxidation effects and a preparation method thereof.The composition is prepared from galactosamine modified astragalus membranaceus exosome, schisandra chinensis nanocrystals, liquorice-chitosan nanoparticles, lactobacillus acidophilus freeze-dried powder, beta-(1, 3) / (1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate) sourced from saccharomyces cerevisiae, and beta-(1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate). And (6) a dextran, grape exosome-curcumin hybrid vesicle freeze-dried powder, a curcumin-mitochondrial targeting peptide compound, a resveratrol-hydroxypropyl-beta-cyclodextrin inclusion compound and reduced panthenol. According to the liver-protecting and anti-oxidation composition disclosed by the invention, multi-dimensional intervention on liver protection is realized through an elaborately designed ternary system. The system is composed of a traditional Chinese medicine extract, a microorganism-plant exosome combination and a high-bioavailability antioxidant, all the parts have a synergistic effect, and the liver protection effect is remarkably improved.

The invention relates to the technical field of recombinant protein expression, in particular to recombinant human-derived III-type collagen as well as a preparation method and application thereof. The invention provides a recombinant human source III type collagen. The amino acid sequence of the recombinant human source III type collagen is shown as SEQ ID NO. 1. The recombinant human-derived III-type collagen prepared by the invention can be better applied to a human body, better exerts biological functions of the collagen, avoids repulsive interaction, is high in safety, can effectively promote cell proliferation and oxidation resistance, and is high in expression yield, stable in batches and good in quality; the recombinant human III type collagen prepared by the invention has the potential of being applied to preparation and production of biological medicines and cosmetics.