The invention discloses spirit-raising and brain-strengthening flour comprising the following raw materials in parts by weight: 100-110 parts of wheat flour, 30-40 parts of glutinous rice, 30-40 parts of millet, 20-30 parts of sorghum, 1-2 parts of mythic fungus, parts of eucommia ganoderma lucidum, 1-2 parts of vitex negundo, 3-4 parts of white lotus, 5-6 parts of ginkgo biloba, 25-30 parts of papaya, 20-26 parts of cranberry, 22-24 parts of cucumber, 30-33 parts of tuna fish meat, 18-23 parts of tofu, 30-35 parts of pure mellow wine, 8-10 parts of truffle, 12-16 parts of cocoa bean, 12-15 parts of sweet osmanthus, 1-2 parts of a food additive and the proper amount of water; the spirit-raising and brain-strengthening flour uses the wheat flour, glutinous rice, millet and sorghum as main raw materials, the prepared flour is good in taste, comprehensive in nutrition, easy to digest and absorb, suitable for processing a variety of flour food. The truffle has the effects of conditioning endocrine, preventing senile dementia and atherosclerosis, resisting tumor cell toxicity, enhancing immunity, resisting aging, resisting fatigue and the like. The sweet osmanthus has the effects of dispelling cold, breaking slag, preventing phlegm from forming, stopping coughing, nourishing face, beautifying, and the like. The tuna fish meat has the effects of protecting the liver, strengthening the liver function, preventing hardening of the arteries, lowering cholesterol content, activateing brain cells, improving human metabolism and the like.