72 results about "Downstream processing" patented technology

Methods, systems and apparatus are described for classifying one or more candidate molecule drugs in relation to one or more desired target conditions for use in downstream processing of drug optimization. A candidate molecule drug inference dataset is received for one or more molecule drugs, the candidate molecule drug dataset comprising data representative of a set of physiochemical values for each candidate molecule drug and a set of pharmacokinetic (PK) values associated with a dosage regimen and target in relation to each candidate molecule drug, and where each candidate molecule drug of the candidate molecule drug dataset is classified as a large molecule drug. For each candidate molecule drug of the candidate molecule drug inference dataset, the corresponding set of physiochemical values and set of PK values for the dosage regimen and target are applied to a machine learning (ML) model configured for classifying whether each candidate molecule drug meets one or more desired conditions associated with pharmacological or pharmacodynamic effects. The ML model is trained using an ML algorithm to train model parameters defining the ML model for classifying whether each candidate molecule drug meets the one or more desired conditions based on a training dataset comprising data representative of a plurality of molecule drugs, each molecule drug represented by a corresponding set of physiochemical values and a set of PK values associated with the dosage regimen and the target, and annotated in relation to corresponding pharmacological or pharmacodynamic effects, responses and / or values in relation to the desired conditions. A set of candidate molecule drugs satisfying the desired conditions is output for downstream processing of drug optimization.

The invention discloses a method for improving the soluble expression of terminal deoxynucleotidyl transferase (TdT), and relates to the technical field of protein engineering and gene engineering, in particular to a method for improving the soluble expression of the TdT. TdT (TdT-B) with the BRCT structural domain removed serves as initial enzyme, hydrophilic amino acid replacement is conducted on hydrophobic amino acid on the surface of the TdT-B enzyme, and a TdT mutant is obtained. The mutant does not need any fusion tag and can be rapidly expressed under induction conditions of high temperatures such as 30 DEG C and 37 DEG C, the soluble protein expression level is remarkably improved, a large amount of soluble TdT is obtained in a short time, the soluble expression level of the TdT is remarkably improved, the fusion tag cutting step is not needed in subsequent downstream treatment, and the preparation method is simple and low in cost.

It has been discovered that the reliance of additional chemical processing facilities downstream of a waste plastic pyrolysis facility may be avoided by utilizing a pyrolysis facility that can both pyrolyse and crack a waste plastic feedstock to thereby form various recycle content products. More specifically, a plastic liquification system and a pyrolysis reactor operating at more severe temperatures and conditions may effectively pyrolyze and crack a waste plastic so that additional downstream processing in a cracking facility may be avoided. Consequently, the waste plastic pyrolysis configuration and process disclosed herein can obtain process efficiencies and logistical simplicity not obtainable in previous waste plastic pyrolysis scheme iterations.

Processes and facilities for producing a recycled content organic chemical compound directly or indirectly from waste plastic. Processing schemes are described herein for converting waste plastic (or hydrocarbon having recycled content derived from waste plastic) into useful intermediate chemicals and final products. The waste plastic can be liquified before being introduced to downstream processing. In some aspects, recycled content aromatics (r-aromatics) can be processed to provide recycled content paraxylene (r-paraxylene), which can then be used to provide recycled content terephthalic acid (r-TPA) and / or recycled content polyethylene terephthalate (r-PET).

The present invention describes a fermentation process with specifically designed medium for improved production of pharmaceutically important drug (actinomycin D) using actinobacterial strain Streptomyces marokkonensis MTCC-25511, isolated from cold deserts of NW Himalayas (Barchey) India. The process involves 12 days fermentation under specific fermentation conditions i.e. temperature 28ºC±2ºC, pH7.0±2.0 with 10% inoculum size followed by downstream processing involving purification of actinomycin D by conventional solvent as well as single step purification method. The direct single step purification involved binding of the metabolites of fermented broth with macroporous adsorbent resin and elution of pure compound Actinomycin D using methanol / water / acetone. The present invention provided high productivity i.e. ~16.7mg / L / h of actinomycin D with >95% purity.

The invention relates to an embedded heat stabilizer for PVC (polyvinyl chloride) as well as a preparation method and application of the embedded heat stabilizer, which can be applied to the field of downstream processing and application of PVC. The preparation method comprises the following steps: firstly, synthesizing a Zn-Sm structure precursor with a hydrotalcite structure through a hydrothermal method, and then carrying out modification treatment through stearic acid to synthesize the Zn-Sm embedded type heat stabilizer. The thermal stabilizer has a relatively better structure and more active centers, so that the adsorption and conversion of HCl in the PVC thermal decomposition process are facilitated, the oxidation resistance is improved through the efficient synergistic effect of rare earth elements samarium and zinc which are introduced into a plurality of orbits and have larger radiuses, and the zinc burning phenomenon caused in the PVC thermal decomposition process is prevented.

Software and hardware that can be used to perform quality control at various stages of a biopharmaceutical production process (100), e.g., during upstream or downstream processing (USP, DSP). In some examples, the disclosed Raman-based solutions enable real-time or near real-time quantification of protein concentration in various units of the biopharmaceutical production equipment, including but not limited to bioreactors, product holding vessels (105), and fluid-transfer lines. In some other examples, the disclosed Raman-based solutions enable real-time or near real-time elucidation and monitoring of the secondary structure of the protein, as a quality marker. In at least some examples, the equipment includes an electronic controller configured to perform or initiate an equipment- or process-control action based on the concentration measurements and / or evaluation of the secondary structure.

The invention is directed to compositions and processes concerning efficient downstream processing of products derived from organic acids pretreatment of plant materials.

The present invention relates to a recombinant bacterium producing Arctic-derived β-glucosidase and its application, belonging to the field of biotechnology. The target gene of the recombinant bacterium is β-glucosidase derived from Arctic bacteria Arc12, the nucleotide sequence of which is shown in SEQ ID No: 1, and the nucleotide sequence of the β-glucosidase with signal peptide is shown in SEQ ID No: 2, the signal peptide is Bacillus subtilis signal peptide CitH, and the expression vector is Escherichia coli-Bacillus shuttle plasmid pMA5, which contains constitutive promoter P HpaII The expression strain is Bacillus subtilis WB600. The present invention provides a method for secretory expression of the enzyme under specific promoter, signal peptide, and strain conditions. This method is simple to operate and can be achieved without the need for special inducers. Secretory expression of the enzyme eliminates the need for cell disruption in downstream processing, reducing production costs and possessing promising application prospects.

In situ Raman spectroscopy methods and systems are provided for the characterization or quantification of a protein purification intermediate and / or the final concentrated fraction during production or preparation. In one embodiment, in situ Raman spectroscopy is used to characterize or quantify critical quality attributes of protein purification intermediates during downstream processing (i.e., after the protein purification intermediate has been obtained). For example, the described in situ Raman spectroscopy methods and systems can be used to characterize and quantify protein purification intermediates as the protein purification intermediates are purified, condensed, or otherwise formulated into the final pharmaceutical product to be marketed or administered.

The invention discloses a method for catalyzing decarboxylation halogenation of renewable carboxylic acid by using non-coenzyme dependent peroxidase. Comprising the following steps: adding a substrate, peroxidase, hydrogen peroxide and potassium halide into a buffer solution containing an organic solvent, and stirring and reacting at normal temperature to obtain the halide. The substrate is monomer benzoic acid obtained after lignin is degraded. The peroxidase does not depend on cofactors and is wide in source and low in production cost. The method is simple in preparation process, mild in reaction condition, high in substrate conversion rate and good in reaction selectivity. And the downstream treatment process is simple.

The invention provides a mutant R41D / S193N of 7beta-hydroxysteroid oxidative dehydrogenase, a gene sequence of the mutant enzyme, a recombinant expression vector containing the gene sequence of the enzyme and an engineering strain containing the vector. The invention also provides a construction method of a recombinant expression vector and an engineering strain, a method for preparing the mutant enzyme by using the engineering strain, and application of the mutant enzyme in in-vitro enzyme catalysis of 7-ketolithocholic acid to produce ursodeoxycholic acid. Compared with a wild type, the R41D / S193N mutant provided by the invention shows stronger preference on a cofactor NADH, is more economical than the traditional NADPH utilization, is simple and convenient in preparation process, high in yield and simple in downstream treatment process, does not involve other solvents except water in the preparation process, belongs to a green biosynthesis method, and is suitable for industrial production. Meanwhile, the method has relatively high atom economy and step economy.

A method of dissolving plastic waste with a petroleum solvent includes mixing the plastic waste and the petroleum solvent at a mixing temperature of greater than a melting point of the plastic waste to form a homogenous mixture. The petroleum solvent includes greater than 50 wt.% aromatics. The mixture may be fed to a downstream processing unit.

The present specification relates to compositions, devices, apparatus, methods, kits and systems for sample preparation (e.g., separation, reduction or removal of small molecules from biomolecules in a sample). Exemplary small molecules that can be separated, reduced or removed have a molecular weight range of <2000 Da. and may include, but are not limited to, dyes, biotin, affinity tags, crosslinkers, reducing agents, labels, nanoparticles, radioactive ligands, mass tags, unreacted molecules and combinations, intermediates and derivatives of the foregoing. Exemplary biomolecules present in a sample, include but are not limited to, proteins, glycoproteins, antibodies, peptides, nucleic acids, polysaccharides, carbohydrates and lipids. Methods, compositions, kits, devices, apparatus and systems of the disclosure may advantageously provide superior separation of small molecule contaminants and additionally reduce time and expenses related to separation of small molecules from larger biomolecules in samples. Biomolecules separated as set forth herein are amenable to better downstream processing.

The invention relates to a method for automatically determining at least one quality parameter characterizing the processing of a carcass part, comprising the following steps: conveying the carcass part as an input product in a conveying direction to a first processing machine (11) by means of a conveying device (10), a first processing machine for performing a first processing step for processing the carcass portion into an output product; acquiring input sensor data of the carcass part by means of at least one sensor device (12) prior to performing the first processing step; classifying partial data related to the machining quality of the first machining step from the input sensor data; determining the at least one quality parameter based on the partial data; and providing the at least one quality parameter for display and / or transmission to a downstream processing machine, the downstream processing machine being adapted to perform at least one subsequent processing step based on the determined quality parameter. The invention also relates to a corresponding device.

This invention relates to multimers containing a plurality of target peptide sequences which permit high levels of expression and improved solubility for ease of downstream processing and purification.

The present technology provides a method of eluting an analyte of interest from an immunoaffinity column. The method of the present technology provides a simplified method for dissociating the bound analyte from the immunoaffinity column and allows direct downstream treatment of the eluent.

In situ Raman spectroscopy methods and systems for characterizing or quantifying a protein purification intermediate and / or final concentrated pool during production or manufacture are provided. In one embodiment, in situ Raman spectroscopy is used to characterize or quantify protein purification intermediates critical quality attributes during downstream processing (i.e., after harvest of the protein purification intermediate). For example, the disclosed in situ Raman spectroscopy methods and systems can be used to characterize and quantify protein purification intermediates as the protein purification intermediates are purified, condensed, or otherwise formulated into the final drug product to be sold or administered.

In situ Raman spectroscopy methods and systems for characterizing or quantifying a protein purification intermediate and / or final concentrated pool during production or manufacture are provided. In one embodiment, in situ Raman spectroscopy is used to characterize or quantify protein purification intermediates critical quality attributes during downstream processing (i.e., after harvest of the protein purification intermediate). For example, the disclosed in situ Raman spectroscopy methods and systems can be used to characterize and quantify protein purification intermediates as the protein purification intermediates are purified, condensed, or otherwise formulated into the final drug product to be sold or administered.

An in-situ Raman spectroscopy method and system are provided for characterizing or quantifying protein purification intermediates and / or final concentrate pools during preparation or manufacture. In one embodiment, in-situ Raman spectroscopy is used to characterize or quantify critical quality attributes of protein purification intermediates during downstream processing (i.e., after harvesting the protein purification intermediates). For example, the disclosed in-situ Raman spectroscopy method and system can be used to characterize and quantify the protein purification intermediates when the protein purification intermediates are purified, concentrated, or otherwise formulated into a final drug product for sale or administration.

A kind of continuous non-stop processing device for fitting long stem vegetables harvesting.Solve the existing device difficult to meet the freshness of long stem vegetables keeps the continuous processing mode, the different downstream processing scene to the difference of packaging specification and the problem of unable to adapt to multi-class operation.The utility model discloses a shell, pre-harvesting assembly, conveying component, weighing assembly, bundling assembly, collection box, bottom moving piece, storage basket, two hand push rods, shell is sequentially provided with pre-harvesting assembly, conveying component, weighing assembly, bundling assembly and collection box along its length direction, the bottom moving piece is arranged in the lower portion of shell, collection box is arranged in the lower portion of shell, the both sides of shell are respectively provided with a hand push rod, and the conical material collecting shell is arranged on shell;Pre-harvesting assembly, conveying component, weighing assembly, bundling assembly and collection box are driven by shell and bottom moving piece to make the continuous processing process of long stem vegetables such as harvesting, weighing, bundling and collection.

A system for processing a hydrocarbon feed has a final stage reactor and an internal separator with a cyclonic separator that forms a substantially gaseous stream and a substantially non-gaseous stream. The substantially gaseous stream is sent directly from the final stage reactor and separator to further downstream processing.

The present invention provides a continuous upstream manufacturing process for the production of bispecific antibody products, which comprise at least two binding domains. The process comprises at least the steps of (i) providing in a perfusion bioreactor at least one mammalian cell culture, which is capable of expressing the bispecific antibody product, (ii) growing the mammalian cell culture at a first perfusion rate until a set point viable cell density is reached, and (iii) maintaining perfusion culture at a second perfusion rate, wherein the bispecific antibody product concentration in the bioreactor is kept below a threshold value. The bispecific antibody product is then subject to subsequent downstream processing. Moreover, the invention provides a bispecific antibody product produced by the continuous upstream manufacturing process.

The utility model discloses automatic sea cucumber processing equipment which comprises a conveying mechanism used for conveying sea cucumbers, a cutting mechanism used for splitting the sea cucumbers and a cleaning mechanism used for cleaning intestines of the sea cucumbers, and the conveying mechanism comprises a carrier and a conveying piece used for conveying the carrier along a conveying path. The carrier is provided with a containing groove for containing sea cucumbers, and the containing groove is fusiform; a cutting opening communicated with the accommodating groove is formed in the lower part of the carrier; the cutting mechanism and the cleaning mechanism are respectively arranged on the transmission path, the cleaning mechanism is arranged at the downstream of the cutting mechanism along the transmission direction of the carrier, the processing equipment can realize automatic processing and can maintain the stability of sea cucumbers, and the processing effect and the processing quality are ensured.

The current invention provides an improved manufacturing process, in particular improved downstream processing, of pharmaceutical products especially comprising proteins, useful for the prevention or treatment of leishmaniosis. The pharmaceutical composition may comprise a protein chimera Q, which is the product of a chimeric gene encoding the antigenic determinants of four proteins of Leishmania infantum.

The present invention provides an improved manufacturing process for producing a bispecific antibody product comprising at least two binding domains. The process comprises at least a process comprising a step of refolding a construct, wherein one or more domains comprise disulfide bonds, and wherein the construct is contacted with a refolding buffer to refold the construct into its native form, the buffer comprising (i) an oxidizing and / or reducing agent at a concentration of at least 0.1 mM, and (ii) a chaotropic agent at a concentration > 1 M, and wherein the pH of the refolding buffer corresponds to + / - 4.5 of the pi value of the first domain or + / - 4.0 of the pi value of the entire construct.

Provided herein are methods of purifying proteins of interest using continuous counter-current downstream processing. Also provided herein are methods of purifying proteins of interest using continuous counter-current affinity nanoparticle dialysis.

The utility model discloses a vacuum suction hopper for modified plastic particles. The vacuum suction hopper comprises a hopper body, an upper cover, a supporting frame, an ion fan, a feeding disc, a vibration motor and a discharging pipe. An upper cover is arranged on the upper portion of the hopper body, an opening is formed in the middle of the upper cover, an ion fan is arranged on the upper portion of the upper cover, a feeding disc is arranged on the side wall of the hopper body, a supporting frame is arranged on the lower portion of the hopper body, a vibration motor is arranged on the side wall of the hopper body, and the lower end of the hopper body is connected with one end of a discharging pipe. The other end of the discharging pipe is connected with a vacuum pump and downstream processing equipment; static electricity of plastic particles in the hopper body can be quickly eliminated through the arrangement of the ion fan, the probability that the plastic particles are adsorbed on the inner wall of the hopper body is reduced, the situation that the plastic particles block a conveying channel due to electrostatic adsorption is avoided, and the plastic particles adsorbed on the inner wall of the hopper body can be vibrated off through the arrangement of the vibration motor; and the possibility of adsorption and accumulation of the plastic particles is further reduced.

A method to produce a nutrient-dense and flavorful fermented shelf stable food product are described. The method comprises using defined fungal and bacterial strains in a solid-state fermentation process of legumes, grains and / or seeds. Following fermentation under specified environmental conditions, minimal downstream processing is performed to preserve the flavors generated via fermentation. This resulting product can be used to make nutritious, highly flavorful food products.

Software and hardware that can be used to perform quality control at various stages of a biopharmaceutical production process, e.g., during upstream or downstream processing. In some examples, the disclosed Raman-based solutions enable real-time or near real-time quantification of protein concentration in various units of the biopharmaceutical production equipment, including but not limited to bioreactors, product holding vessels, and fluid-transfer lines. In some other examples, the disclosed Raman-based solutions enable real-time or near real-time elucidation and monitoring of the secondary structure of the protein, as a quality marker. In at least some examples, the equipment includes an electronic controller configured to perform or initiate an equipment- or process-control action based on the concentration measurements and / or evaluation of the secondary structure.