35 results about "Downstream processing" patented technology

Processes and facilities for producing a recycled content organic chemical compound directly or indirectly from waste plastic. Processing schemes are described herein for converting waste plastic (or hydrocarbon having recycled content derived from waste plastic) into useful intermediate chemicals and final products. The waste plastic can be liquified before being introduced to downstream processing. In some aspects, recycled content aromatics (r-aromatics) can be processed to provide recycled content paraxylene (r-paraxylene), which can then be used to provide recycled content terephthalic acid (r-TPA) and / or recycled content polyethylene terephthalate (r-PET).

The invention relates to an embedded heat stabilizer for PVC (polyvinyl chloride) as well as a preparation method and application of the embedded heat stabilizer, which can be applied to the field of downstream processing and application of PVC. The preparation method comprises the following steps: firstly, synthesizing a Zn-Sm structure precursor with a hydrotalcite structure through a hydrothermal method, and then carrying out modification treatment through stearic acid to synthesize the Zn-Sm embedded type heat stabilizer. The thermal stabilizer has a relatively better structure and more active centers, so that the adsorption and conversion of HCl in the PVC thermal decomposition process are facilitated, the oxidation resistance is improved through the efficient synergistic effect of rare earth elements samarium and zinc which are introduced into a plurality of orbits and have larger radiuses, and the zinc burning phenomenon caused in the PVC thermal decomposition process is prevented.

In situ Raman spectroscopy methods and systems are provided for the characterization or quantification of a protein purification intermediate and / or the final concentrated fraction during production or preparation. In one embodiment, in situ Raman spectroscopy is used to characterize or quantify critical quality attributes of protein purification intermediates during downstream processing (i.e., after the protein purification intermediate has been obtained). For example, the described in situ Raman spectroscopy methods and systems can be used to characterize and quantify protein purification intermediates as the protein purification intermediates are purified, condensed, or otherwise formulated into the final pharmaceutical product to be marketed or administered.

The invention provides a mutant R41D / S193N of 7beta-hydroxysteroid oxidative dehydrogenase, a gene sequence of the mutant enzyme, a recombinant expression vector containing the gene sequence of the enzyme and an engineering strain containing the vector. The invention also provides a construction method of a recombinant expression vector and an engineering strain, a method for preparing the mutant enzyme by using the engineering strain, and application of the mutant enzyme in in-vitro enzyme catalysis of 7-ketolithocholic acid to produce ursodeoxycholic acid. Compared with a wild type, the R41D / S193N mutant provided by the invention shows stronger preference on a cofactor NADH, is more economical than the traditional NADPH utilization, is simple and convenient in preparation process, high in yield and simple in downstream treatment process, does not involve other solvents except water in the preparation process, belongs to a green biosynthesis method, and is suitable for industrial production. Meanwhile, the method has relatively high atom economy and step economy.

A method of dissolving plastic waste with a petroleum solvent includes mixing the plastic waste and the petroleum solvent at a mixing temperature of greater than a melting point of the plastic waste to form a homogenous mixture. The petroleum solvent includes greater than 50 wt.% aromatics. The mixture may be fed to a downstream processing unit.

The present specification relates to compositions, devices, apparatus, methods, kits and systems for sample preparation (e.g., separation, reduction or removal of small molecules from biomolecules in a sample). Exemplary small molecules that can be separated, reduced or removed have a molecular weight range of <2000 Da. and may include, but are not limited to, dyes, biotin, affinity tags, crosslinkers, reducing agents, labels, nanoparticles, radioactive ligands, mass tags, unreacted molecules and combinations, intermediates and derivatives of the foregoing. Exemplary biomolecules present in a sample, include but are not limited to, proteins, glycoproteins, antibodies, peptides, nucleic acids, polysaccharides, carbohydrates and lipids. Methods, compositions, kits, devices, apparatus and systems of the disclosure may advantageously provide superior separation of small molecule contaminants and additionally reduce time and expenses related to separation of small molecules from larger biomolecules in samples. Biomolecules separated as set forth herein are amenable to better downstream processing.

The invention relates to a method for automatically determining at least one quality parameter characterizing the processing of a carcass part, comprising the following steps: conveying the carcass part as an input product in a conveying direction to a first processing machine (11) by means of a conveying device (10), a first processing machine for performing a first processing step for processing the carcass portion into an output product; acquiring input sensor data of the carcass part by means of at least one sensor device (12) prior to performing the first processing step; classifying partial data related to the machining quality of the first machining step from the input sensor data; determining the at least one quality parameter based on the partial data; and providing the at least one quality parameter for display and / or transmission to a downstream processing machine, the downstream processing machine being adapted to perform at least one subsequent processing step based on the determined quality parameter. The invention also relates to a corresponding device.

The present technology provides a method of eluting an analyte of interest from an immunoaffinity column. The method of the present technology provides a simplified method for dissociating the bound analyte from the immunoaffinity column and allows direct downstream treatment of the eluent.

A kind of continuous non-stop processing device for fitting long stem vegetables harvesting.Solve the existing device difficult to meet the freshness of long stem vegetables keeps the continuous processing mode, the different downstream processing scene to the difference of packaging specification and the problem of unable to adapt to multi-class operation.The utility model discloses a shell, pre-harvesting assembly, conveying component, weighing assembly, bundling assembly, collection box, bottom moving piece, storage basket, two hand push rods, shell is sequentially provided with pre-harvesting assembly, conveying component, weighing assembly, bundling assembly and collection box along its length direction, the bottom moving piece is arranged in the lower portion of shell, collection box is arranged in the lower portion of shell, the both sides of shell are respectively provided with a hand push rod, and the conical material collecting shell is arranged on shell;Pre-harvesting assembly, conveying component, weighing assembly, bundling assembly and collection box are driven by shell and bottom moving piece to make the continuous processing process of long stem vegetables such as harvesting, weighing, bundling and collection.

The utility model discloses automatic sea cucumber processing equipment which comprises a conveying mechanism used for conveying sea cucumbers, a cutting mechanism used for splitting the sea cucumbers and a cleaning mechanism used for cleaning intestines of the sea cucumbers, and the conveying mechanism comprises a carrier and a conveying piece used for conveying the carrier along a conveying path. The carrier is provided with a containing groove for containing sea cucumbers, and the containing groove is fusiform; a cutting opening communicated with the accommodating groove is formed in the lower part of the carrier; the cutting mechanism and the cleaning mechanism are respectively arranged on the transmission path, the cleaning mechanism is arranged at the downstream of the cutting mechanism along the transmission direction of the carrier, the processing equipment can realize automatic processing and can maintain the stability of sea cucumbers, and the processing effect and the processing quality are ensured.

The current invention provides an improved manufacturing process, in particular improved downstream processing, of pharmaceutical products especially comprising proteins, useful for the prevention or treatment of leishmaniosis. The pharmaceutical composition may comprise a protein chimera Q, which is the product of a chimeric gene encoding the antigenic determinants of four proteins of Leishmania infantum.

The present invention provides an improved manufacturing process for producing a bispecific antibody product comprising at least two binding domains. The process comprises at least a process comprising a step of refolding a construct, wherein one or more domains comprise disulfide bonds, and wherein the construct is contacted with a refolding buffer to refold the construct into its native form, the buffer comprising (i) an oxidizing and / or reducing agent at a concentration of at least 0.1 mM, and (ii) a chaotropic agent at a concentration > 1 M, and wherein the pH of the refolding buffer corresponds to + / - 4.5 of the pi value of the first domain or + / - 4.0 of the pi value of the entire construct.

Provided herein are methods of purifying proteins of interest using continuous counter-current downstream processing. Also provided herein are methods of purifying proteins of interest using continuous counter-current affinity nanoparticle dialysis.

This invention provides a functional nano-predispersed slurry for fertilizers and its preparation method, relating to the fields of agricultural chemistry and new materials technology. The slurry is a stable suspension comprising a continuous liquid phase and a dispersed phase. The dispersed phase is a natural nanotube-shaped mineral carrier, on which a plant-derived active composition and an amino acid metal chelate are co-loaded. The natural nanotube-shaped mineral carrier is halloysite nanotubes, which constitute 3%-6% of the slurry by mass. The functional nano-predispersed slurry for fertilizers and its preparation method provided by this invention have the advantages of excellent stability, functional integration and synergistic effects, strong adaptability to downstream processing, standardization, and traceability.

A method for the optimisation of feed for downstream processing includes passing a sulphide ore concentrate to a first leach step, in which a leach discharge slurry is produced, passing an intermediate product to a second leach step, in which a pregnant leach solution is produced, and combining the leach discharge slurry of the fist leach step with the intermediate product in the second leach step. Residual acid generated in the first leach step is used to leach the intermediate product in the second leach step and provides the pregnant leach solution containing one or more target metals.

The application discloses a kind of neutralization reaction pH value accurate measurement method and device.The method includes: acid in acid storage tank is pumped into pipeline mixer with directly sent waste lye and is mixed and reacts;Product 1# waste lye leaves pipeline mixer, wherein gas phase 1# acid gas enters upward degassing pipeline, liquid phase 2# waste lye enters downward liquid pipeline;2# waste lye is sent into degassing tank gas-liquid separation, separated 2# acid gas is mixed into downstream processing with 1# acid gas;Neutralized waste lye of gas-liquid separation is sent into stripping tank stripping, 3# acid gas obtained and waste lye after neutralization are sent into downstream processing respectively;The pH value of 2# waste lye is detected using the pH value detector arranged on the horizontal pipe of downward liquid pipeline, and the acid injection amount of acid pump into pipeline mixer is adjusted based on the pH value.The application has the advantages of simple process, stable operation, high detection accuracy, low operation energy consumption and cost, and sensitive adjustment.

The utility model discloses a feeding device for solder paste processing, which relates to the technical field of solder paste processing and comprises a conveying pipe fitting and a flexible pipe structure, the flexible pipe structure is mounted at one end of the conveying pipe fitting, an assembly pipe fitting is mounted at one end, far away from the flexible pipe structure, of the conveying pipe fitting, and a feeding hopper is mounted at the top of the assembly pipe fitting. And a material pushing assembly is mounted in the middle of the assembling pipe fitting. According to the feeding device for solder paste processing, the electric heating pipes are annularly distributed and installed between the outer pipe body and the inner pipe body which are of a double-layer structure, by means of operation of the structures of the electric heating pipes, it can be guaranteed that materials conveyed in the conveying pipe fitting are kept in a molten state to the maximum extent, and the situation that subsequent processing goes wrong due to the unnecessary condensation problem is avoided; and due to the structural arrangement of the flexible pipe structure, the structural flexibility of connection between the whole device and downstream processing equipment can be ensured to the greatest extent, and the limitation of connection between the equipment caused by structural interference is avoided.

A method for separating chemical impurities from a feed includes providing a fermentation broth comprising a blend of carboxylic acids, adjusting the pH of the broth to a more acidic pH, heating the fermentation broth to create water vapor from the water in the broth, providing an organic extraction solvent having a density less than the density of water, condensing the water vapor into condensed water droplets containing cyclohexane carboxylic acid, extracting the cyclohexane carboxylic acid from the condensed water droplets into an organic extraction solvent layer by contacting the condensed water vapor droplets with the organic extraction solvent and separating the organic extraction solvent layer containing the cyclohexane carboxylic acid from the aqueous layer containing the carboxylic acid impurities. The method results in the separation and removal of unwanted impurities in the feed from a target compound and provides a high yield of the target compound for downstream processing.

This invention relates to multimers containing a plurality of target peptide sequences which permit high levels of expression and improved solubility for ease of downstream processing and purification.

The invention discloses a visual screening method for planting termitomyces albuminosus for processing, and relates to the technical field of food processing pretreatment and online quality grading. Interface prior and raw material quality evaluation indexes are obtained through micro-fog wetting dynamic imaging and four-dimensional space-time body representation; according to the method, the evaporation flux, the gas-phase mass transfer coefficient and the boundary layer thickness are inversed by combining a knife edge type Schriz light path, a two-section type humidity step is adaptively set, a memory curve fingerprint and processing suitability evaluation index is calculated by a controlled recursion model, and the bioactive components are estimated through a prediction relation established through offline calibration. Evaluating the suitability of the collybia albuminosa sample purposes by adopting a multi-purpose suitability scoring model, outputting a purpose classification result and a process parameter range of a corresponding purpose, issuing process parameters to downstream processing equipment, receiving quality monitoring data to form feedback, independently correcting a parameter mapping relation according to the type of the purpose, and determining the quality of the collybia albuminosa sample. And a full-chain closed loop of raw material evaluation, parameter generation, processing execution and quality feedback is realized.

The present invention relates to the field of upstream and downstream processing and provides a process for producing a purified rhabdovirus from a cell culture, preferably a purified oncolytic rhabdovirus and in particular a vesicular stomatitis virus, including pharmaceutical compositions comprising the rhabdovirus.

The invention relates to the field of organic synthesis, in particular to a cyclic olefin compound as well as a preparation method and application thereof. According to the method, a cyclic alkene compound and cyclopentadiene are taken as raw materials and subjected to a Diels-Alder reaction under the liquid phase condition at the temperature of 100-200 DEG C and the pressure of 1-5 MPa, the molar ratio of the cyclic alkene compound to the cyclopentadiene is controlled to be (1-2): 1, the reaction can be carried out in a tubular reactor, a kettle reactor or a combined reactor in an intermittent, semi-continuous or continuous mode, and a compound II can be supplemented during the reaction so as to guarantee the constant molar ratio of the raw materials. According to the method, a dicyclopentadiene high-temperature cracking step in a traditional process is omitted, the reaction temperature is low, byproducts are few, the utilization rate of raw materials and the green environmental protection property of a device are improved, and energy consumption and danger are reduced. The cyclic olefin compound prepared by the method is used for ring-opening metathesis polymerization, and the hydrogenated ring-opening metathesis polymer obtained after hydrogenation has excellent elongation at break and is suitable for downstream processing application.

Provided is a method for producing an organic compound, the method making it possible to ensure an adequate reaction time and obtain a targeted substance at a high yield even in an organic reaction that requires a relatively long time to complete the reaction. A method for producing an organic compound, wherein the method is characterized in that: a fluid processing apparatus F used in the production method is equipped with an upstream processing unit that processes a fluid to be processed between at least two processing surfaces 1 and 2 that relatively rotate, and a downstream processing unit disposed downstream of the upstream processing unit, the downstream processing unit being provided with a plurality of labyrinth seals that function to retain and stir the fluid to be processed that has been processed by the upstream processing unit; due to the fluid to be processed, which contains at least one type of organic compound, being passed through the upstream processing unit, the fluid to be processed is subjected to upstream processing; due to the fluid to be processed that has been subjected to upstream processing being passed through the downstream processing unit, the fluid to be processed that has been subjected to upstream processing is subjected to downstream processing; and the upstream processing and the downstream processing are performed continuously.

Methods, systems and apparatus are described for estimating titer of immunoglobulin single variable domains (ISVDs) The method includes: receiving an ISVD sequence dataset comprising data representative of at least one ISVD sequence; processing the received ISVD sequence dataset with a protein large language model configured for generating an ISVD embeddings dataset; processing the ISVD embeddings dataset with a ML model configured for generating an estimated ISVD titer value for each ISVD sequence of the ISVD sequence dataset based on the ISVD embeddings dataset input; and outputting data representative of the ISVD titer values corresponding to the ISVD sequence dataset for downstream processing.

A method to produce a nutrient-dense and flavorful fermented shelf stable food product are described. The method comprises using defined fungal and bacterial strains in a solid-state fermentation process of legumes, grains and / or seeds. Following fermentation under specified environmental conditions, minimal downstream processing is performed to preserve the flavors generated via fermentation. This resulting product can be used to make nutritious, highly flavorful food products.

A method of separating chemical impurities from a feed includes an ion exchange chromatography separation step to remove a first impurity from the feed and an ion exchange adsorption-desorption separation step to remove a second impurity from the feed. The separation method results in the separation and removal of unwanted impurities present in the feed from a target compound and provides a high yield of the target compound for further downstream processing.

The invention discloses a method for synthesizing azo compounds by catalyzing hydrazine compounds through non-coenzyme-dependent oxidase. Comprising the following steps: adding a hydrazine compound, oxidase and hydrogen peroxide into a buffer solution containing an organic solvent, and stirring and reacting at normal temperature to obtain the azo compound. The structure of the hydrazine compound is as follows: R < 1 >-NH-NH-R < 2 >, wherein R1 and R2 are any one of phenyl or substituted phenyl; the oxidase does not depend on non-nicotinoyl cofactors and is wide in source and low in production cost. The method is simple in preparation process, mild in reaction condition, high in substrate conversion rate, good in reaction selectivity and simple in downstream treatment process.

Provided in the present invention are a fully automatic multifunctional pilot platform system and a chromatographic system thereof. The platform system comprises a first input main pipe and a second input main pipe; the first input main pipe is connected to a plurality of input pipes, and is controlled to be turned on and off by means of corresponding valves. The second input main pipe is also connected to said plurality of input pipes, and is controlled to be turned on or off by corresponding valves. Outlet ends of the plurality of input pipes are respectively connected to corresponding liquid inlet ports of a movable mounting module. The movable mounting module is further provided with a plurality of return ports and a plurality of liquid outlet ports. The liquid outlet ports are respectively connected to a plurality of collection pipes by means of output pipes. In the chromatographic system, an RTF column is installed in the movable mounting module. When in use, two tangential flow chromatography columns are used and operate alternately: one is in a loading mode, and the other is in an elution mode. The present invention has the advantages of modularity, achieving a plurality of functions for different process requirements, and satisfying the needs of biopharmaceutical downstream processing workflows.

Selective hydrogenation processes are disclosed that can upgrade impure feeds, such as those obtained from biomass and containing a number of small (e.g., 2-6 carbon atom) molecules having aldehyde and / or ketone carbon atoms. For example, whereas glycolaldehyde and its methylated derivative, hydroxyacetone (acetol) are both high value intermediates for certain downstream processing reactions, they are normally recovered in a condensate from pyrolysis of carbohydrates (e.g., aldose-containing sugars) together with glyoxal and its methylated derivative, pyruvaldehyde. The selective hydrogenation of these compounds bearing two carbonyl carbon atoms, without over-hydrogenation to ethylene glycol and propylene glycol, can increase the concentration of the desired intermediates. These beneficial effects of selective hydrogenation may be achieved through the use of a hydrogenation catalyst comprising noble metals such as Ru and Pt.

The invention relates to a cellobiose 2-epimerase mutant with high catalytic efficiency and application of the cellobiose 2-epimerase mutant. The mutant is obtained by changing the specific amino acid site of a wild type enzyme, the double mutant psyCE-FR shows the optimal performance, and the catalytic efficiency (Kcat / Km) of the double mutant is improved by about 40% compared with that of the wild type enzyme. The mutant has excellent catalytic efficiency and substrate affinity at low temperature (4-10 DEG C), and is suitable for low-temperature dairy product processing and functional sugar preparation. Besides, the mutant retains the characteristic that the psychrophilic enzyme is easy to thermally inactivate, can be instantly inactivated at 50 DEG C, is compatible with a pasteurization process in the dairy industry, reduces the downstream treatment cost, and improves the product purity and safety. The invention also provides a gene for coding the mutant, a recombinant expression vector, a recombinant engineering bacterium and a method for producing epilactose by using the mutant enzyme, and the mutant enzyme has wide application prospect and commercial value.