Methods and devices for determining the concentration of an
analyte in a physiological sample are provided. In the subject methods, the physiological sample is introduced into an
electrochemical cell having a working and
reference electrode. A first
electric potential is applied to the
cell and the
resultant cell current over a period of time is measured to determine a first time-current transient. A second
electric potential of opposite polarity is then applied and a second a time-current transient is determined. The preliminary concentration of the
analyte is then calculated from the first and / or second time-current transient. This preliminary
analyte concentration less a background value is then multiplied by a
hematocrit correction factor to obtain the analyte concentration in the sample, where the
hematocrit correction factor is a function of the preliminary analyte concentration and the variable γ of the
electrochemical cell. The subject methods and devices are suited for use in the determination of a wide variety of analytes in a wide variety of samples, and are particularly suited for the determination of analytes in
whole blood or derivatives thereof, where an analyte of particular interest is glucose.