2411 results about "Electrophoreses" patented technology

The present disclosure provides electrophoretic systems, methods and compositions for spatial analysis, which can serve to magnify or demagnify spatial resolution of analytes of interest that are captured using electrophoresis. Some implementations can use a diverging or converging electric field in an electrophoretic capture system. Such a divergent or convergent electric field, as opposed to a parallel electric field, can be generated by, for example, utilizing different sizes of electrodes associated with or imbedded in substrates. Also provided herein are electrophoretic systems, methods and compositions for spatial analysis, which can serve to selectively migrate one or more analytes from a region of interest in the biological sample for capture using electrophoresis.

An electrophoretic system is provided for analyte capture from a biological sample. The electrophoretic system can be used to permeabilize the sample to allow analytes to be released from the sample. For example, the sample can be contacted with capture probes attached to a substrate, and an electric field created by the electrophoretic system can cause analytes to be released from the cell, and effectively migrate toward and bind to the capture probes attached to the substrate.

The invention relates to the technical field of analysis of physical and chemical properties of soil, in particular to a dry farming soil fertility rapid detection and fertility improvement method, which comprises the following steps: sorting a colloidal phase and a dissolved phase of a humic acid complex based on density difference and dielectric property difference through a gradient centrifugation and dielectrophoresis enriched physical field synergistic separation technology; a two-channel detection time sequence is optimized by adopting a genetic algorithm, a non-overlapping time sequence signal flow is generated by taking a signal interference suppression ratio as a fitness function, and time domain cross interference is suppressed. A programmable resistance-capacitance compensation model is constructed, parallel capacitor array parameters are dynamically adjusted to correct electrochemical phase angle offset, and a three-dimensional fertility distribution curved surface and a residual map are generated in combination with a Kriging interpolation model. On the basis of residual spectrum spatial distribution characteristics, a cross-linked slow-release system is deployed to improve the organic matter binding capacity of a humic acid deficiency area, a mesoporous zeolite-based passivator is applied to regulate and control an ion migration path, a closed-loop feedback mechanism is formed through dynamic phase response monitoring, and accurate detection and directional remediation of soil fertility are achieved.

A switchable light modulating device with an electrophoretic medium disposed between a first light-transmissive electrode layer and a second electrode layer. The device includes one or more apodization structures in a bottom of a cell that contains the electrophoretic fluid, and the apodization structures reduce optical interference when the cell is in an open state and the structure is viewed with reflected or transmitted light passing through the apodization structure.

The invention relates to the field of biological detection, in particular to a primer group and a kit for fusing round cell sarcoma by using EWSR1 non-ETS and a use method. The EWSR1 non-ETS fused round cell sarcoma detection primer group comprises nine primer sequences, namely a primer group A and a primer group B. The primer group A and the primer group B are used for detecting EWSR1 non-ETS fused round cell sarcoma. According to the invention, the variation types of the EWSR1 non-ETS fusion sarcoma are divided into five variation types, the five fusion variation types are divided into two groups, two groups of upstream and downstream amplification primer groups are designed in a targeted manner, and each fusion variation type can be amplified to obtain a target product peak with the size of 100-250 bp, so that the target product peak can be amplified to obtain a target product peak with the size of 100-250 bp. The primer provided by the invention can be used for detecting five variation types fused by three types of genes at one time. Through combined application of the primer group, multiple fluorescent PCR and high-resolution capillary electrophoresis, the lowest detection limit is 10 copies, and the sensitive and efficient detection method for the EWSR1 non-ETS fused round cell sarcoma is provided and has the characteristics of simplicity, convenience, accurate result and economic benefit.

Rosa chinensis gray mold is a main disease in the cultivation process of rosa chinensis, and seriously threatens the quality and yield of cut-flower rosa chinensis. In order to effectively cope with the disaster stress which influences the yield and quality of the Chinese rose, 192 pairs of SSR primers are used in the research to carry out PCR amplification on DNA of parents' Jinmai '' Beijing red 'and 6 randomly selected offspring. 35 pairs of primers show genetic difference between male and female parents. The 35 pairs of primers are further used for carrying out PCR amplification on filial generation DNA, detection is carried out through PAGE electrophoresis, and an electrophoretic band is converted into a 0, 1 matrix for genetic typing. The method comprises the following steps: selecting an optimal subset through correlation regression analysis, and finally successfully screening out three primers, namely, Geno-BC-1, Geno-BC-2 and Geno-BC-3, which are related to gray mold resistance. The discovery provides an important theoretical basis for molecular marker-assisted Chinese rose disease-resistant breeding, and is expected to promote early identification of disease-resistant plants, so that the breeding period is shortened, and the breeding efficiency is improved.

The invention belongs to the technical field of biomedical engineering, and particularly relates to a preparation method and application of a titanium dental implant surface coating, and the preparation method comprises the following steps: S1, pretreatment of a titanium dental implant matrix: sequentially carrying out grinding and polishing, ultrasonic cleaning and acid etching treatment; s2, preparation of a coating precursor solution: adding nano-hydroxyapatite powder, chitosan, gelatin and titanium dioxide nanoparticles into deionized water, stirring to form a suspension, then adding a silver nitrate solution, and continuously stirring to form the coating precursor solution; s3, coating a coating: coating the surface of the pretreated titanium dental implant matrix with the coating precursor solution by adopting an electrophoretic deposition method; and S4, drying and sintering: drying and sintering the coated titanium dental implant. According to the preparation method of the titanium dental implant surface coating, provided by the invention, the bonding strength between the coating and the titanium dental implant matrix can be improved.

The invention relates to the field of biological detection, in particular to a mini STR (short tandem repeat) double-multiplex amplification system and kit for degrading 25 gene loci of a sample. The invention provides a kit. The kit comprises 25 gene loci; the 25 gene loci comprise 23 autosomal STR gene loci, one sex gene locus and one Yindel gene locus. According to the kit provided by the invention, the primers are divided into a group A and a group B, and all gene loci are miniS TR gene loci on the basis of using current mainstream instruments and equipment. The two tubes of primers use the same experiment condition, and amplification and electrophoresis can be carried out at the same time. Compared with the existing miniSTR kit, the kit disclosed by the invention can be independently amplified and does not need to be combined with other autosomal STR kits for use, and the typing of 25 gene loci can be obtained through one-time detection. The effective detection rate of degraded samples is greatly improved, and technical support is provided for detection of difficult cases.

The invention discloses a heat allocation system based on a heat pump. The heat allocation system comprises a water tank; a water source heat pump; an air source heat pump; a cooling tower; a cooling water circulation system; a hot water circulation system; a chilled water circulation system; an exhaust heat recovery system; wherein the water tank, the water source heat pump, the air source heat pump, the cooling tower, the cooling water circulation system, the hot water circulation system, the chilled water circulation system and the exhaust heat recovery system are connected with one another to realize heat allocation, especially heat allocation of a coating pretreatment electrophoresis production line of a vehicle body or parts forming the vehicle body; according to the system, heat generated when the heat pump / refrigerating unit produces chilled water and / or heat taken away in exhaust air can be recycled and used for heating the heat using tail end. Therefore, the heat which is wasted originally can be fully utilized, the energy consumption is reduced, and the utilization efficiency of energy is improved.

The invention discloses an electronic paper display panel, a driving method thereof and a display device, and mainly relates to the technical field of display, a first pixel unit comprises a first upper driving electrode, a first electrophoresis accommodating cavity and a first lower driving electrode, and the first upper driving electrode and the first lower driving electrode are arranged on the two sides of the first electrophoresis accommodating cavity respectively; the first pixel unit further comprises first light absorbing particles, second light absorbing particles and first light reflecting particles which are arranged in the first electrophoresis accommodating cavity; the charge quantity of the first light absorbing particles, the charge quantity of the first light reflecting particles and the charge quantity of the second light absorbing particles are sequentially reduced, the polarities of the first light absorbing particles and the second light absorbing particles are the same, and the polarities of the first light absorbing particles and the first light reflecting particles are opposite. Therefore, the electronic paper display panel can be switched between double-sided display and single-sided display, and the image resolution during single-sided display is improved.

The invention discloses application of Indel marker dosage typing in detection of sweet potato soft rot resistance, and belongs to the technical field of plant genetic engineering. The Indel molecular marker related to the sweet potato soft rot resistance is obtained through screening, the nucleotide sequence of the Indel molecular marker is shown as SEQ ID NO.2, and an 8bp sequence is inserted into or deleted from the 161st site of the sequence shown as SEQ ID NO.2. Experiments find that target bands of amplification products are obtained through amplification of the Indel molecular marker and electrophoresis, dosage values of two genotypes are obtained according to gray values of the dyed electrophoretic bands, and results show that the insertion type Indel molecular marker is positively correlated with the sweet potato soft rot resistance. The method is of great significance to the dose effect research and molecular assisted breeding of sweet potato gene functions.

The invention discloses a preparation method of a raspberry-derived exosome loaded with neroli essential oil. The preparation method comprises the following steps: preparing raspberry fruits into homogenate; centrifuging, collecting supernate, adjusting the pH value to 4.07.5, standing, and centrifuging again; taking supernate, adjusting the pH value to 7.2-7.4, adding polyethylene glycol, standing, centrifuging, collecting precipitate, and carrying out resuspension dispersion; performing microfiltration to obtain supernate, and combining electrophoresis dialysis and ultrafiltration concentration to obtain the high-purity raspberry exosome. And diluting the exosome, adding the diluted exosome into a dimethyl sulfoxide solution containing neroli essential oil, carrying out water bath stirring, carrying out ultrafiltration centrifugation, and taking a clear liquid to obtain the exosome loaded with the neroli essential oil. Efficient separation and purification of the raspberry exosome are achieved by applying an isoelectric precipitation technology, then the neroli essential oil is wrapped in the exosome, the stability of the essential oil is improved, the action time is prolonged, meanwhile, the neroli essential oil and active ingredients of the exosome generate a synergistic effect, and the antioxidant activity is jointly enhanced.

The invention discloses a BDD electrode material and a preparation method thereof. The preparation method comprises the following core steps: pretreating the surface of a porous titanium substrate; depositing a nanoscale TiN film layer on the surface of the pretreated porous titanium substrate in advance; depositing a layer of single-walled carbon nanotube network on the TiN thin film layer by using an electrophoretic deposition method; growing a highly-doped BDD layer on the single-walled carbon nanotube network by adopting a plasma enhanced chemical vapor deposition technology; repeating the two steps to form a multi-layer SWCNT / BDD composite structure, and controlling the thickness of the multi-layer SWCNT / BDD composite structure to be 1-50 microns; and finally, depositing a low-doped BDD active layer with the thickness of 1-5 microns by adopting a hot filament chemical vapor deposition (HFCVD) technology. According to the pulse laser assisted preparation process provided by the invention, the femtosecond laser pulse is introduced to replace traditional plasma treatment, so that the process flow is simplified, and the efficiency is improved; according to the technology, the process is simplified, the quality of the TiN film is improved through accurate energy input, and a new way is opened up for further improving the performance of the BDD electrode.

The invention relates to the technical field of image data processing, in particular to an automobile part electrophoresis machining surface detection method based on machine vision. The method comprises the following steps: dividing an electrophoresis machining surface image of the automobile part into a plurality of sub-blocks, and obtaining an initial clustering result of the electrophoresis machining surface image of the automobile part through an agglomerated hierarchical clustering method; according to the minimum bounding rectangle size of each edge in the sub-block and the number of edge pixel points in the sub-block, the primary fiber property of the sub-block is obtained; calculating fiber factors of the sub-blocks; and carrying out secondary cutting on the initial clustering result based on the fiber factor of each sub-block in the initial clustering result to obtain an electrophoresis processing surface detection result of the automobile part, so that the accuracy of the electrophoresis processing surface detection result of the automobile part is effectively improved.

The present invention provides a lipoprotein subtype detection kit, a preparation method and an application thereof, belonging to the technical field of biological detection. In the separating gel of the kit, BODIPY 505 / 515 is mixed, so that staining is not required during detection, and direct sample loading and electrophoresis can be carried out, reducing the operation steps and shortening the operation time, and improving the detection sensitivity. In addition, N,N'-bisacryloylpiperazine is mixed in both the separating gel and the stacking gel. The rigidity of the N,N'-bisacryloylpiperazine gel is used to reduce gel deformation and trailing phenomena during migration, significantly improving the resolution, and enhancing the stability and repeatability of detection. The detection kit provided by the present invention has the advantages of short detection operation time, high detection sensitivity, high resolution, good stability and repeatability.

The invention discloses a low-temperature curing high-throwing-power cathode electrophoretic coating and a preparation method and application thereof, and belongs to the field of automobile coating materials, the coating is composed of epoxy resin, a fluorosilicone copolymerization type epoxy modifier, a polyamino siloxane-acrylic acid hybrid and other components, and performance improvement is achieved through the synergistic effect of the two brand-new modifiers. Wherein the fluorine-silicon copolymerization type epoxy modifier is prepared by taking bisphenol A epoxy resin as a framework, introducing fluorine-containing alkyl and siloxane groups and carrying out ultraviolet reaction, so that the surface energy is reduced, and low-temperature crosslinking is promoted; the polyamido siloxane-acrylic acid hybrid takes siloxane as a core, and acrylic acid and polyamido are bridged through isocyanate, so that the crosslinking density and the water solubility are enhanced. When the coating is prepared, the two modifiers are mixed with epoxy resin, a solvent and the like, and the coating is prepared through dispersion and dilution. The coating can be cured at low temperature, so that a uniform thick film is obtained in an inner cavity of a complex workpiece, and meanwhile, the coating has excellent corrosion resistance and adhesive force and is suitable for efficient and environment-friendly coating of an automobile body and parts.

An electrophoretic system is provided for analyte capture from a biological sample. The electrophoretic system can be used to permeabilize the sample to allow analytes to be released from the sample. For example, the sample can be contacted with capture probes attached to a substrate, and an electric field created by the electrophoretic system can cause analytes to be released from the cell, and effectively migrate toward and bind to the capture probes attached to the substrate.

The invention relates to the technical field of electrophoresis wastewater treatment, in particular to a frame electrophoresis wastewater discharge treatment system which comprises a wastewater treatment box and a sediment collection box, the sediment collection box is located on the outer side of the wastewater treatment box, and multiple sets of movable isolation sediment structures are arranged in the wastewater treatment box. The movable isolation precipitation structure comprises two outer cylinders and two inner cylinders, the two inner cylinders are respectively inserted into the two outer cylinders, and the two ends of each outer cylinder respectively penetrate through the inner walls of the two sides of the wastewater treatment box in a damping rotation manner. According to the invention, the height of the liquid level in the wastewater treatment box can be detected through the liquid level induction wireless controller, so that the interlayer isolation plates located below the liquid level in the wastewater treatment box are all in a horizontal state, and the interior of the wastewater treatment box located below the liquid level is divided into a plurality of spaces; during precipitation, floccules in different spaces are respectively precipitated on the upper surfaces of the corresponding interlayer isolation plates, and the floccules are precipitated in a layered manner, so that the floccules are effectively prevented from being blocked.

The present invention discloses a black chicken liver hydrolyzed peptide that can be used to improve ulcerative colitis, and its preparation method and application, which belong to the field of biomedicine technology. The preparation method of the black chicken liver hydrolyzed peptide comprises the following steps: making black chicken liver into minced meat and mixing it with water, heating the obtained liquid material, adding alkaline protease for enzymatic hydrolysis, inactivating the enzyme and collecting the supernatant by centrifugation, and freeze-drying to obtain the black chicken liver hydrolyzed peptide. The present invention adopts formaldehyde titration to detect the degree of hydrolysis, adopts SDS-PAGE electrophoresis and Tricine-SDS-PAGE electrophoresis to detect the molecular weight, performs sequence analysis by LC-MS / MS, uses a database to predict the biological function of peptide segments, detects the antioxidant and blood pressure lowering ability of black chicken liver peptide by in vitro experiments, and detects the effect of black chicken liver peptide in improving colitis by mouse experiments. The results show that the black chicken liver peptide prepared by the present invention has the advantages of antioxidant, blood pressure lowering and improvement of ulcerative colitis.

This invention is related to the field of biosensors. In particular, devices and methods are described which enable the quantification of biomolecules by virtue of particle speed driven by induced-charge electrophoresis. For example, a range of biomolecules can be simultaneously detected in a highly sensitive manner from a library (e.g., combination) of active particles having different shapes or fluorescent signatures. Such biomolecules are related to conditions including, but not limited to, cancer biomarkers, viral infection biomarkers, toxins, pesticides, and small molecule drugs.

The invention discloses a modified carbon fiber, a carbon fiber composite material and a preparation method and application thereof, relates to the technical field of carbon fiber surface modification, and solves the problems that in the prior art, a carbon fiber modification method is single, and the damage condition of the carbon fiber composite material cannot be monitored in real time. The preparation method comprises the following steps: immersing desized carbon fiber cloth into conductive filler dispersion liquid, and drying to obtain conductive filler modified carbon fiber cloth; immersing the conductive filler modified carbon fiber cloth into a poly (3, 4-ethylenedioxythiophene) / polystyrene sulfonate electrolyte for electrophoretic deposition; taking the conductive filler modified carbon fiber cloth as a negative electrode and a graphite plate as a positive electrode, and performing vacuum drying after deposition to obtain conductive polymer nanoparticle deposited carbon fibers; and preparing the carbon fiber composite material from the modified carbon fiber and epoxy resin. The synergistic effect of the conductive filler and the conductive polymer enhances the acting force between the carbon fiber and the resin, thereby enhancing the mechanical properties and damage detection capability of the material. The method can be applied to the field of damage detection.

The utility model discloses metal plate electrophoresis pretreatment equipment for uniform treatment, which belongs to the technical field of metal plate pretreatment and comprises a tank body, a sliding rail, a sliding block, a fixed block and a cross rod are arranged at the upper end of the groove body All the sliding blocks on each side are connected through a shear fork support. A reciprocating swing mechanism is arranged at the lower end of the groove body and used for pushing the frame body of the hanger to swing in a reciprocating mode. By means of the mode, a user can keep the locking effect on one sliding block and push any movable sliding block to move on the sliding rail, all the sliding blocks are expanded or contracted on the sliding rail at equal intervals, and therefore the distance between the transverse rods is adjusted to adapt to hanging frames of different sizes, and the cleaning requirements for metal plates of different specifications are met; the frame body of the hanging frame is pushed through the reciprocating swing mechanism, the frame body swings on the transverse rod in a reciprocating mode through the hooks, the metal plate on the frame body can continuously disturb cleaning liquid in the tank body, impurities on the surface of the metal plate can be separated more easily, and the cleaning efficiency and the cleaning effect are effectively improved.

The invention discloses a 26 Multi-InDel genetic marker system for highly degraded sample typing, a detection primer and application of the 26 Multi-InDel genetic marker system, and belongs to the technical field of forensic medicine identification. According to the invention, through bioinformatics screening and experimental verification, a multi-InDel genetic marker system of which the lengths of 26 amplicons do not exceed 125 bp is constructed. The problem that degradation detection material detection and high system efficiency are difficult to consider at the same time is solved. The random matching probability of the system in Han population in Hunan reaches 2.25 * 10 <-17 >, the cumulative paternity exclusion rate is 0.999925, complete typing of highly degraded DNA can be achieved, triad paternity test can be completed, and a new technical scheme which is efficient and compatible with a capillary electrophoresis platform is provided for forensic practice.

The invention discloses a device and method for automatically shearing and recycling agarose gel, and relates to the technical field of agarose gel cutting, and the device and method for automatically shearing and recycling agarose gel comprise an image acquisition unit, a three-axis moving mechanism, a gel cutting device, an electrophoresis apparatus and a control unit; the image acquisition unit comprises a light source assembly and an image acquisition camera corresponding to an irradiation area, the image acquisition camera is used for shooting a dyed electrophoresis image, and the light source assembly is used for obliquely irradiating gel to enable a sample strip to develop color; the control unit comprises an analysis processing unit; the analysis processing unit comprises a deep learning detection module and a data processing module; the control unit cooperates with the image acquisition unit, the three-axis moving mechanism and the gel cutting device, so that a complete automatic process from strip identification and positioning after electrophoresis to gel block cutting, grabbing, releasing and recycling is constructed.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW2; the KASP marker RW2 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 526653. According to the application, the KASP molecular marker RW2 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW2 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention belongs to the technical field of photovoltaic materials, and provides a photovoltaic profile and a preparation method and application thereof. The preparation method comprises the following steps: carrying out surface conditioning and phosphorization on a steel substrate (carbon steel or recycled steel or low alloy steel), carrying out electrophoretic deposition by using epoxy thermosetting electrophoretic primer, and pre-drying; the outer surface of a workpiece is coated with fluorocarbon finish paint (the fluorocarbon finish paint comprises the following raw materials in specific content: polyester resin with a certain viscosity hydroxyl value, fluorocarbon resin with a certain viscosity hydroxyl value, pigment filler, an organic solvent, a wetting leveling agent, a dispersing agent, a defoaming agent, an adhesion promoter and an aliphatic isocyanate curing agent), and curing is performed. And an electrophoretic primer layer with the thickness of 15-25 microns and a fluorocarbon finish layer with the thickness of 20-30 microns are formed on the outer surface of the steel substrate through curing. According to the photovoltaic profile, the electrophoretic primer layer and the fluorocarbon finish paint layer are formed on the low-cost steel substrate through one-time curing, the process can be simplified, the cost can be reduced, the paint layer is thin, and the photovoltaic profile further has long-term excellent adhesive force, aging resistance, corrosion resistance, weather resistance, gloss retention and the like.

The invention relates to the technical field of automobile part testing, and discloses a tool and method for testing slippage and residual torsion of a toothed automobile body sleeve, and the tool comprises a fixing tool, a testing bolt, a nut, a large-head flange electrophoresis plate, a positioning clamping block assembly, and a ballast cushion block. In a testing state, the large-head flange electrophoresis plate is installed on the fixing tool; a positioning area in the positioning clamping block assembly is used for positioning the installation position of the vehicle body sleeve, the test bolt and the positioning clamping block assembly jointly guarantee concentricity, self-centering compensation can be achieved without deflection, the precision of a test result is high, the test bolt is installed, the test bolt is combined with a nut, and a ballast cushion block is used for applying pressure to the vehicle body sleeve according to set parameters; the testing tool is simple in structure and low in cost, assembling work before testing is easy to operate, testing can be completed only by using a universal electronic testing machine in a matched mode, and the requirement for detection equipment is low.

The invention discloses a device and a method for a circulating cooling water system, an electrophoresis device comprises an electrophoresis tank, a reaction tank and a sedimentation tank which are communicated in sequence, a cathode material is arranged in the electrophoresis tank, an electrophoresis anode tube group is inserted on the cathode material, the electrophoresis anode tube group is composed of a plurality of electrophoresis anode tubes which are arranged in parallel, and the electrophoresis anode tubes are arranged in the sedimentation tank. The electrophoresis anode tube is provided with an anode element, water inlet pipes, an anode tab and a water outlet pipe, the anode element is coated with an electrophoresis film, the top of the anode element is provided with a connecting pipe fitting, the water inlet pipes, the anode tab and the water outlet pipe penetrate through the connecting pipe fitting, and each water inlet pipe is communicated with a bypass water outlet of the circulating cooling water system; each water outlet pipe is communicated with a first water return port of the circulating cooling water system, a water inlet of the electrophoresis tank is communicated with the bypass water outlet, and a water outlet of the sedimentation tank is communicated with a second water return port. The electrophoresis method is implemented by adopting the electrophoresis device. According to the electrophoresis device and the electrophoresis method, the current density and the current efficiency can be improved, and descaling of medium and large circulating water systems is met.

An electrophoretic display device includes an ambient light sensor and a front light system for adaptively recovering whiteness and balancing color on the display.

The invention discloses a self-assembly-based DNA molecular weight standard preparation method, which comprises: S1, chemically synthesizing two single-stranded DNAs having the same length but not completely complementary, and each of the two single-stranded DNAs is composed of two parts of sequences: one part is a complementary sequence, and the other part is a self-assembly complementary sequence; s2, mixing in a reaction buffer solution for directional self-assembly, and adding ligase; s3, reacting at room temperature for 0.5-10 minutes, regulating and controlling the number of self-assembly times by controlling the reaction time, and increasing the base number equal to the base number of the single-stranded DNA in each connection to form a plurality of DNA molecular weight standards with sequence lengths gradually increased in an equal difference manner; s4, carrying out thermal inactivation treatment; and S5, adding an electrophoresis loading buffer solution to prepare a DNA molecular weight standard, and storing at low temperature. The DNA molecular weight standard is rapidly prepared through the one-pot method, and the method has the advantages of being simple in process, low in manufacturing cost, high in expansibility and the like and has good application prospects.