3249 results about "Electrophoreses" patented technology

The present disclosure provides electrophoretic systems, methods and compositions for spatial analysis, which can serve to magnify or demagnify spatial resolution of analytes of interest that are captured using electrophoresis. Some implementations can use a diverging or converging electric field in an electrophoretic capture system. Such a divergent or convergent electric field, as opposed to a parallel electric field, can be generated by, for example, utilizing different sizes of electrodes associated with or imbedded in substrates. Also provided herein are electrophoretic systems, methods and compositions for spatial analysis, which can serve to selectively migrate one or more analytes from a region of interest in the biological sample for capture using electrophoresis.

The present disclosure provides electrophoretic systems, methods and compositions for spatial analysis, which can serve to magnify or demagnify spatial resolution of analytes of interest that are captured using electrophoresis. Some implementations can use a diverging or converging electric field in an electrophoretic capture system. Such a divergent or convergent electric field, as opposed to a parallel electric field, can be generated by, for example, utilizing different sizes of electrodes associated with or imbedded in substrates. Also provided herein are electrophoretic systems, methods and compositions for spatial analysis, which can serve to selectively migrate one or more analytes from a region of interest in the biological sample for capture using electrophoresis.

An electrophoretic system is provided for analyte capture from a biological sample. The electrophoretic system can be used to permeabilize the sample to allow analytes to be released from the sample. For example, the sample can be contacted with capture probes attached to a substrate, and an electric field created by the electrophoretic system can cause analytes to be released from the cell, and effectively migrate toward and bind to the capture probes attached to the substrate.

The present invention provides the method for the separation of at least more than two Low molecular weight (LMWs) impurities present in the protein mixture comprising fusion protein CTL4-IgG1 by using Capillary Electrophoresis-Sodium dodecyl sulfate (CE-SDS) wherein the loading amount of protein mixture is more than 270 μg. The invention further provides a ratio of protein to sodium dodecyl sulfate is 2.7:1 or higher.

The invention provides a KASP molecular marker related to a corn ear height character under a drought condition and application of the KASP molecular marker, and belongs to the technical field of molecular genetics. The SNP marker site related to the ear height character under the drought condition and having polymorphism is obtained through whole genome association analysis, the SNP marker site refers to a corn B73 RefGenv4 genome, when nucleotide polymorphism T / C exists at the position 147243591 of a 10 # chromosome, the SNP marker site is a dominant character, and nucleotide sequences used for amplifying a KASP molecular marker primer group are shown as SEQ ID NO.1-3. Gene typing identification can be rapidly, simply and efficiently carried out by utilizing a KASP typing technology, enzyme digestion and electrophoresis are not needed, accurate identification and selection can be carried out in a seedling stage, a large amount of breeding workload can be reduced, the period of high-ear-position breeding selection can be shortened, and the method can be used for high-ear-position molecular marker-assisted breeding of corn.

The invention relates to the technical field of analysis of physical and chemical properties of soil, in particular to a dry farming soil fertility rapid detection and fertility improvement method, which comprises the following steps: sorting a colloidal phase and a dissolved phase of a humic acid complex based on density difference and dielectric property difference through a gradient centrifugation and dielectrophoresis enriched physical field synergistic separation technology; a two-channel detection time sequence is optimized by adopting a genetic algorithm, a non-overlapping time sequence signal flow is generated by taking a signal interference suppression ratio as a fitness function, and time domain cross interference is suppressed. A programmable resistance-capacitance compensation model is constructed, parallel capacitor array parameters are dynamically adjusted to correct electrochemical phase angle offset, and a three-dimensional fertility distribution curved surface and a residual map are generated in combination with a Kriging interpolation model. On the basis of residual spectrum spatial distribution characteristics, a cross-linked slow-release system is deployed to improve the organic matter binding capacity of a humic acid deficiency area, a mesoporous zeolite-based passivator is applied to regulate and control an ion migration path, a closed-loop feedback mechanism is formed through dynamic phase response monitoring, and accurate detection and directional remediation of soil fertility are achieved.

A switchable light modulating device with an electrophoretic medium disposed between a first light-transmissive electrode layer and a second electrode layer. The device includes one or more apodization structures in a bottom of a cell that contains the electrophoretic fluid, and the apodization structures reduce optical interference when the cell is in an open state and the structure is viewed with reflected or transmitted light passing through the apodization structure.

The invention relates to the field of biological detection, in particular to a primer group and a kit for fusing round cell sarcoma by using EWSR1 non-ETS and a use method. The EWSR1 non-ETS fused round cell sarcoma detection primer group comprises nine primer sequences, namely a primer group A and a primer group B. The primer group A and the primer group B are used for detecting EWSR1 non-ETS fused round cell sarcoma. According to the invention, the variation types of the EWSR1 non-ETS fusion sarcoma are divided into five variation types, the five fusion variation types are divided into two groups, two groups of upstream and downstream amplification primer groups are designed in a targeted manner, and each fusion variation type can be amplified to obtain a target product peak with the size of 100-250 bp, so that the target product peak can be amplified to obtain a target product peak with the size of 100-250 bp. The primer provided by the invention can be used for detecting five variation types fused by three types of genes at one time. Through combined application of the primer group, multiple fluorescent PCR and high-resolution capillary electrophoresis, the lowest detection limit is 10 copies, and the sensitive and efficient detection method for the EWSR1 non-ETS fused round cell sarcoma is provided and has the characteristics of simplicity, convenience, accurate result and economic benefit.

The invention discloses fluorescent electrophoretic ink and a display device, and relates to the technical field of electrophoretic display. The fluorescent electrophoretic ink comprises an electrophoretic medium, and first electrophoretic particles, second electrophoretic particles and third electrophoretic particles dispersed in the electrophoretic medium; the first electrophoretic particle has a first charge polarity, the second electrophoretic particle has a second charge polarity, and the third electrophoretic particle has the first charge polarity or the second charge polarity; the first electrophoretic particles present a first color under visible light irradiation, and the second electrophoretic particles present a second color under visible light irradiation; and the third electrophoretic particles are in a colorless transparent state or a semitransparent state under the irradiation of visible light, and are in fluorescence emission of a third color under the irradiation of invisible light with a preset wavelength. The display effect of fluorescent electrophoresis display is improved.

Rosa chinensis gray mold is a main disease in the cultivation process of rosa chinensis, and seriously threatens the quality and yield of cut-flower rosa chinensis. In order to effectively cope with the disaster stress which influences the yield and quality of the Chinese rose, 192 pairs of SSR primers are used in the research to carry out PCR amplification on DNA of parents' Jinmai '' Beijing red 'and 6 randomly selected offspring. 35 pairs of primers show genetic difference between male and female parents. The 35 pairs of primers are further used for carrying out PCR amplification on filial generation DNA, detection is carried out through PAGE electrophoresis, and an electrophoretic band is converted into a 0, 1 matrix for genetic typing. The method comprises the following steps: selecting an optimal subset through correlation regression analysis, and finally successfully screening out three primers, namely, Geno-BC-1, Geno-BC-2 and Geno-BC-3, which are related to gray mold resistance. The discovery provides an important theoretical basis for molecular marker-assisted Chinese rose disease-resistant breeding, and is expected to promote early identification of disease-resistant plants, so that the breeding period is shortened, and the breeding efficiency is improved.

The invention provides a display panel, a display device and a preparation method of the display panel, the display panel comprises a first substrate, a color ink layer, a plurality of pixel electrodes and an electrophoresis layer, the color ink layer is arranged on one side of the first substrate, the color ink layer comprises a plurality of ink parts, the plurality of ink portions include first ink portions and second ink portions of different colors. The plurality of pixel electrodes are arranged on one side of the first substrate, and the pixel electrodes comprise first electrodes corresponding to the first ink parts and second electrodes corresponding to the second ink parts. The electrophoresis layer is arranged on the side, away from the first substrate, of the color ink layer and the pixel electrodes, the electrophoresis layer comprises electrophoresis particles, and at least part of the orthographic projection of the first ink part on the first substrate is located between the orthographic projections of the two first electrodes on the first substrate. The orthographic projection of the second ink part on the first substrate is at least partially located between the orthographic projections of the two second electrodes on the first substrate.

The invention belongs to the technical field of biomedical engineering, and particularly relates to a preparation method and application of a titanium dental implant surface coating, and the preparation method comprises the following steps: S1, pretreatment of a titanium dental implant matrix: sequentially carrying out grinding and polishing, ultrasonic cleaning and acid etching treatment; s2, preparation of a coating precursor solution: adding nano-hydroxyapatite powder, chitosan, gelatin and titanium dioxide nanoparticles into deionized water, stirring to form a suspension, then adding a silver nitrate solution, and continuously stirring to form the coating precursor solution; s3, coating a coating: coating the surface of the pretreated titanium dental implant matrix with the coating precursor solution by adopting an electrophoretic deposition method; and S4, drying and sintering: drying and sintering the coated titanium dental implant. According to the preparation method of the titanium dental implant surface coating, provided by the invention, the bonding strength between the coating and the titanium dental implant matrix can be improved.

The invention relates to the field of biological detection, in particular to a mini STR (short tandem repeat) double-multiplex amplification system and kit for degrading 25 gene loci of a sample. The invention provides a kit. The kit comprises 25 gene loci; the 25 gene loci comprise 23 autosomal STR gene loci, one sex gene locus and one Yindel gene locus. According to the kit provided by the invention, the primers are divided into a group A and a group B, and all gene loci are miniS TR gene loci on the basis of using current mainstream instruments and equipment. The two tubes of primers use the same experiment condition, and amplification and electrophoresis can be carried out at the same time. Compared with the existing miniSTR kit, the kit disclosed by the invention can be independently amplified and does not need to be combined with other autosomal STR kits for use, and the typing of 25 gene loci can be obtained through one-time detection. The effective detection rate of degraded samples is greatly improved, and technical support is provided for detection of difficult cases.

The utility model relates to the technical field of automobile part processing, in particular to automobile part surface pretreatment equipment before electrophoresis, which comprises a degreasing tank, support columns and a bearing disc, support plates are arranged at two ends of the front side of the degreasing tank, and a reciprocating screw rod and a slide rail are respectively arranged between the support plates from top to bottom. A motor is arranged on one side of the supporting plate, a T-shaped connecting plate is arranged between the reciprocating lead screw and the sliding rail, a supporting column is arranged on the top of one side of the T-shaped connecting plate, a mounting plate is arranged on the top of the supporting column, and a connecting pipeline is arranged on the top of the mounting plate. The automobile parts are driven by a waterproof air cylinder to move downwards into an oil and rust removing agent in a degreasing tank to be soaked, after grease in the surfaces is removed, a rotary damping drive mounting plate and a spraying device are rotated to move to the top of a bearing disc, a water pump sucks a water source in a water tank, and the water source is sprayed to the surfaces of the automobile parts through a connecting pipeline and the spraying device. And the residual oil and rust removing agent is cleaned.

The invention discloses a molecular biological method capable of simultaneously detecting duck-derived and goose-derived components and application of the molecular biological method, after genome DNA of a sample to be detected is subjected to PCR amplification and capillary electrophoresis through primers shown in SEQ ID NO.1 and SEQ ID NO.2, sequence comparison is carried out, when the peak value is 999bp, it is indicated that the sample contains the duck-derived component, and when the peak value is 992bp, it is indicated that the sample contains the goose-derived component, and when the peak value is 992bp, it is indicated that the sample contains the goose-derived component, it is indicated that the sample contains the duck-derived component. When the peak values are 999bp and 992bp, the duck-derived component and the goose-derived component are contained at the same time. The detection kit developed on the basis of the method can generate considerable economic benefits and good social values.

The invention discloses a heat allocation system based on a heat pump. The heat allocation system comprises a water tank; a water source heat pump; an air source heat pump; a cooling tower; a cooling water circulation system; a hot water circulation system; a chilled water circulation system; an exhaust heat recovery system; wherein the water tank, the water source heat pump, the air source heat pump, the cooling tower, the cooling water circulation system, the hot water circulation system, the chilled water circulation system and the exhaust heat recovery system are connected with one another to realize heat allocation, especially heat allocation of a coating pretreatment electrophoresis production line of a vehicle body or parts forming the vehicle body; according to the system, heat generated when the heat pump / refrigerating unit produces chilled water and / or heat taken away in exhaust air can be recycled and used for heating the heat using tail end. Therefore, the heat which is wasted originally can be fully utilized, the energy consumption is reduced, and the utilization efficiency of energy is improved.

The invention discloses an electronic paper display panel, a driving method thereof and a display device, and mainly relates to the technical field of display, a first pixel unit comprises a first upper driving electrode, a first electrophoresis accommodating cavity and a first lower driving electrode, and the first upper driving electrode and the first lower driving electrode are arranged on the two sides of the first electrophoresis accommodating cavity respectively; the first pixel unit further comprises first light absorbing particles, second light absorbing particles and first light reflecting particles which are arranged in the first electrophoresis accommodating cavity; the charge quantity of the first light absorbing particles, the charge quantity of the first light reflecting particles and the charge quantity of the second light absorbing particles are sequentially reduced, the polarities of the first light absorbing particles and the second light absorbing particles are the same, and the polarities of the first light absorbing particles and the first light reflecting particles are opposite. Therefore, the electronic paper display panel can be switched between double-sided display and single-sided display, and the image resolution during single-sided display is improved.

The invention discloses a multistage treatment electrophoresis sewage treatment device which comprises an outer box body and a display screen mounted on the front side surface of the outer box body, three sets of conveying pump bodies are installed in the lower portion of the outer box body, a primary treatment cavity is formed in the upper portion of the left side of the outer box body in a penetrating mode, a grating body arranged to be of an annular structure is installed in the primary treatment cavity, and the primary treatment cavity is formed above a sedimentation treatment cavity; a dissolved air release mechanism is mounted on the corresponding outer box body above the air flotation treatment cavity; an activated carbon adsorption plate is mounted in the adsorption treatment cavity; a lower supporting plate is mounted on the outer side above the flow guide pipe in a penetrating manner; rotating rods are symmetrically mounted on the outer side of the lower end of the medicine discharging pipe. According to the multistage treatment electrophoresis sewage treatment device, multistage filtration treatment can be conveniently carried out on electrophoresis sewage, so that the electrophoresis sewage filtration treatment effect is improved, subsequent treatment work can be conveniently carried out on the electrophoresis sewage, and the electrophoresis sewage treatment effect is improved.

The invention relates to the technical field of separation and purification, and discloses a method for extracting, separating and purifying serrapeptase in fermentation liquor through two-step salting-out. The serrapeptase in the fermentation liquor is separated and purified through a two-step salting-out extraction technology, specifically, the serrapeptase in the fermentation liquor is concentrated in a lower phase through one-step salting-out extraction, bacterial cell fragments are removed, then the serrapeptase is concentrated and purified through two-step salting-out extraction, and the serrapeptase is enriched in a middle phase. The method has good separation and purification effects, after two steps of salting-out extraction, the purification multiple of the serrapeptase is 3.5 times, the recovery rate is 90.7%, the electrophoresis purity of the obtained serrapeptase is close to 100% through SDS-PAGE gray analysis, and the serrapeptase freeze-dried powder can keep the enzyme activity of 88.6% after being stored for 180 days at the temperature of-80 DEG C.

The invention belongs to the technical field of biomolecules, and particularly relates to an SMAD7 gene for judging chicken sex and application. Through the molecular marker and the design of a new PCR primer, the migration distance difference of two bands amplified by a ZW chromosome is 299bp, and compared with the migration distance difference of 150bp of a traditional sex identification gene CHD1 amplification system, the migration distance difference of the bands of the two items is larger, and judgment is easier; the fragments amplified by the designed PCR primer are smaller and are only 517bp and 816p, the PCR amplification time is remarkably shortened, and the sex identification time is shortened; in addition, amplification and agarose gel electrophoresis can be directly carried out by extracting chicken tissues, the sex of the chicken can be rapidly judged according to an electrophoresis result, the DNA extraction time is shortened, the time length can be controlled within 1 hour, operation is easy, the identification result is accurate, basic-level application and popularization are facilitated, and the method has a wide market application prospect.

Embodiments provide an ink composition, a film manufactured using the ink composition, and an electrophoretic display device including the film. The ink composition includes (A) a semiconductor nanorod, and (B) a dispersion medium, wherein the dispersion medium includes: (B1) a compound which has a viscosity at 25° C. of 70 cps or less and has a dielectric constant of 5 or more; (B2) a compound which has a viscosity at 25° C. of 80 cps or more or is a solid and has a dielectric constant of 5 or more; (B3) a compound which has a viscosity at 25° C. of 20 cps or more or is a solid and has a dielectric constant of less than 5; and (B4) a compound which has a viscosity at 25° C. of 12 cps or less or is a solid and has a dielectric constant of 4 to 7.5.

The invention discloses a copper-based current collector modified by a self-repairing artificial interface layer, a preparation method of the copper-based current collector and application of the copper-based current collector in a negative-electrode-free lithium battery. According to the self-repairing artificial interface layer, an ionomer with a self-repairing function is prepared from a nitrogen-containing water-soluble comonomer, a quaternary ammonium salt monomer, double-bond-terminated polyoxyethylene and a dopamine self-repairing monomer in a solution polymerization mode, and the ionomer with the self-repairing function is prepared into a solution to serve as an electrophoresis solution; and depositing the ionomer on the exposed copper-based current collector by adopting an electrophoretic deposition method. The artificial interface layer obtained by the method is compact and uniform, the thickness is adjustable, and the effects of accelerating lithium ion transmission, homogenizing lithium deposition, self-repairing and the like can be achieved. When the copper-based current collector modified by the self-repairing artificial interface layer is used for the negative-electrode-free lithium battery, the cycle life of the copper-based current collector is obviously prolonged, and a new solution is provided for developing the high-performance negative-electrode-free lithium battery.

The invention discloses a universal primer group for rapidly detecting nucleic acid of stenotrophomonas maltophilia, a kit and application, belongs to the technical field of genetic engineering, and aims to solve the problem that qPCR (quantitative polymerase chain reaction) heating and cooling time needs at least 60 minutes when a sample is traditional stenotrophomonas maltophilia. The primer group comprises a primer group which is composed of nucleotide sequences as shown in SEQ ID NO.1 and SEQ ID NO.2 and aims at conserved region genes of stenotrophomonas maltophilia, detection of stenotrophomonas maltophilia is completed in the same tube system within 30 minutes based on an RPA / RAA constant-temperature amplification method, and a direct electrophoresis method is selected for judgment according to the position of a target band.

The invention discloses application of Indel marker dosage typing in detection of sweet potato soft rot resistance, and belongs to the technical field of plant genetic engineering. The Indel molecular marker related to the sweet potato soft rot resistance is obtained through screening, the nucleotide sequence of the Indel molecular marker is shown as SEQ ID NO.2, and an 8bp sequence is inserted into or deleted from the 161st site of the sequence shown as SEQ ID NO.2. Experiments find that target bands of amplification products are obtained through amplification of the Indel molecular marker and electrophoresis, dosage values of two genotypes are obtained according to gray values of the dyed electrophoretic bands, and results show that the insertion type Indel molecular marker is positively correlated with the sweet potato soft rot resistance. The method is of great significance to the dose effect research and molecular assisted breeding of sweet potato gene functions.

The invention provides a preparation method of a TiO2 / Au-C3N4 heterojunction photoelectrochemical biosensor and an application of the TiO2 / Au-C3N4 heterojunction photoelectrochemical biosensor. Comprising the following steps: step 1, preparing TiO2 / FTO; step 2, preparation of TiO2 / Au-C3N4 (titanium dioxide / Au-C3N4); step 3, preparing a nucleic acid aptamer; and step 4, using the confining liquid mercaptohexanol. The preparation method comprises the following steps: firstly, preparing a TiO2 thin film by an electrophoretic deposition method, and then combining the TiO2 thin film with graphite phase carbon nitride (g-C3N4) doped with high-conductivity noble metal gold nanoparticles (Au NPs) to form a heterojunction; under an illumination condition, Au NPs and photons interact to generate a regional surface plasma resonance phenomenon, and a strong electromagnetic field and high-concentration high-energy carriers are formed on the surface of the nano structure in the process, so that the photocurrent response of the composite material is remarkably enhanced; besides, the introduced Au NPs can also form an Au-S bond with the DNA aptamer containing the-SH group, and more binding sites are provided, so that the capture capability of the aptamer is enhanced.

The invention discloses a preparation method of a raspberry-derived exosome loaded with neroli essential oil. The preparation method comprises the following steps: preparing raspberry fruits into homogenate; centrifuging, collecting supernate, adjusting the pH value to 4.07.5, standing, and centrifuging again; taking supernate, adjusting the pH value to 7.2-7.4, adding polyethylene glycol, standing, centrifuging, collecting precipitate, and carrying out resuspension dispersion; performing microfiltration to obtain supernate, and combining electrophoresis dialysis and ultrafiltration concentration to obtain the high-purity raspberry exosome. And diluting the exosome, adding the diluted exosome into a dimethyl sulfoxide solution containing neroli essential oil, carrying out water bath stirring, carrying out ultrafiltration centrifugation, and taking a clear liquid to obtain the exosome loaded with the neroli essential oil. Efficient separation and purification of the raspberry exosome are achieved by applying an isoelectric precipitation technology, then the neroli essential oil is wrapped in the exosome, the stability of the essential oil is improved, the action time is prolonged, meanwhile, the neroli essential oil and active ingredients of the exosome generate a synergistic effect, and the antioxidant activity is jointly enhanced.

The invention discloses a BDD electrode material and a preparation method thereof. The preparation method comprises the following core steps: pretreating the surface of a porous titanium substrate; depositing a nanoscale TiN film layer on the surface of the pretreated porous titanium substrate in advance; depositing a layer of single-walled carbon nanotube network on the TiN thin film layer by using an electrophoretic deposition method; growing a highly-doped BDD layer on the single-walled carbon nanotube network by adopting a plasma enhanced chemical vapor deposition technology; repeating the two steps to form a multi-layer SWCNT / BDD composite structure, and controlling the thickness of the multi-layer SWCNT / BDD composite structure to be 1-50 microns; and finally, depositing a low-doped BDD active layer with the thickness of 1-5 microns by adopting a hot filament chemical vapor deposition (HFCVD) technology. According to the pulse laser assisted preparation process provided by the invention, the femtosecond laser pulse is introduced to replace traditional plasma treatment, so that the process flow is simplified, and the efficiency is improved; according to the technology, the process is simplified, the quality of the TiN film is improved through accurate energy input, and a new way is opened up for further improving the performance of the BDD electrode.

The invention relates to the technical field of image data processing, in particular to an automobile part electrophoresis machining surface detection method based on machine vision. The method comprises the following steps: dividing an electrophoresis machining surface image of the automobile part into a plurality of sub-blocks, and obtaining an initial clustering result of the electrophoresis machining surface image of the automobile part through an agglomerated hierarchical clustering method; according to the minimum bounding rectangle size of each edge in the sub-block and the number of edge pixel points in the sub-block, the primary fiber property of the sub-block is obtained; calculating fiber factors of the sub-blocks; and carrying out secondary cutting on the initial clustering result based on the fiber factor of each sub-block in the initial clustering result to obtain an electrophoresis processing surface detection result of the automobile part, so that the accuracy of the electrophoresis processing surface detection result of the automobile part is effectively improved.

The present invention provides a lipoprotein subtype detection kit, a preparation method and an application thereof, belonging to the technical field of biological detection. In the separating gel of the kit, BODIPY 505 / 515 is mixed, so that staining is not required during detection, and direct sample loading and electrophoresis can be carried out, reducing the operation steps and shortening the operation time, and improving the detection sensitivity. In addition, N,N'-bisacryloylpiperazine is mixed in both the separating gel and the stacking gel. The rigidity of the N,N'-bisacryloylpiperazine gel is used to reduce gel deformation and trailing phenomena during migration, significantly improving the resolution, and enhancing the stability and repeatability of detection. The detection kit provided by the present invention has the advantages of short detection operation time, high detection sensitivity, high resolution, good stability and repeatability.

The invention discloses a low-temperature curing high-throwing-power cathode electrophoretic coating and a preparation method and application thereof, and belongs to the field of automobile coating materials, the coating is composed of epoxy resin, a fluorosilicone copolymerization type epoxy modifier, a polyamino siloxane-acrylic acid hybrid and other components, and performance improvement is achieved through the synergistic effect of the two brand-new modifiers. Wherein the fluorine-silicon copolymerization type epoxy modifier is prepared by taking bisphenol A epoxy resin as a framework, introducing fluorine-containing alkyl and siloxane groups and carrying out ultraviolet reaction, so that the surface energy is reduced, and low-temperature crosslinking is promoted; the polyamido siloxane-acrylic acid hybrid takes siloxane as a core, and acrylic acid and polyamido are bridged through isocyanate, so that the crosslinking density and the water solubility are enhanced. When the coating is prepared, the two modifiers are mixed with epoxy resin, a solvent and the like, and the coating is prepared through dispersion and dilution. The coating can be cured at low temperature, so that a uniform thick film is obtained in an inner cavity of a complex workpiece, and meanwhile, the coating has excellent corrosion resistance and adhesive force and is suitable for efficient and environment-friendly coating of an automobile body and parts.