461 results about "Brassica" patented technology

The invention provides an anti-premature senility management method for closely-planted double-layer truncated cone macadamia nut trees, and belongs to the technical field of fruit tree cultivation. The premature senility prevention management method comprises layered nutrition supply and flower and fruit protection management. The layered nutrition supply comprises the following steps: deeply applying a calcium magnesium phosphate fertilizer to the lower layer of soil greater than 60cm, and covering a root controller; organic fertilizers and biological agents are applied to 30-60 cm soil of the middle layer; a macroelement, humic acid and seaweed extract water-soluble fertilizer is applied to the 0-30 cm soil of the upper layer; the flower and fruit protection management comprises the steps that ABA and paclobutrazol are sprayed in the flower bud differentiation period; brassinolide and fluid boron are sprayed in the full-bloom stage; spraying forchlorfenuron and amino acid in a young fruit stage; nAA and monopotassium phosphate are sprayed in the fruit swelling period. Layered nutrition supply and flower and fruit protection management are carried out on the densely-planted double-layer truncated cone macadamia nut trees, premature senility of the densely-planted macadamia nut trees is prevented, branch development of the lower layer is promoted, the tree vigor is balanced, and the high-yield level is maintained.

The invention discloses a water and fertilizer management method for transplanted high-yield indica type two-line hybrid rice, and belongs to the technical field of rice cultivation. Comprising the following steps: (1) applying citric acid chelated calcium magnesium phosphate fertilizer to soil before transplanting; (2) applying coated controlled-release urea and a silicon-zinc fertilizer on the third day after transplanting; (3) when the tiller number reaches 2650,000-2750,000 plants / hm < 2 >, starting intermittent light field drying for three times, and deeply applying potassium magnesium sulphate before field drying; (4) laterally and deeply applying urea and a boron-humic acid chelate when tips of two inverted leaves are exposed; (5) spraying an aqueous solution in a full heading stage, wherein the aqueous solution comprises monopotassium phosphate, chelated zinc and brassinolide. Through staged precise fertilization, special chelating / controlled release fertilizer and water and fertilizer-field drying cooperation, the method has the advantages that the fertilizer utilization rate is increased, the nutritional requirements of rice in each growth period are precisely matched, and water and fertilizer cooperative management is optimized.

The invention discloses development and application of a molecular marker of a rape stain tolerance related gene BnaERF3, and belongs to the technical field of molecular biology. According to the invention, the BnaA06. ERF3 gene is screened on the basis of whole genome associated molecules, and is used as a key gene in QTL (qWL.A06) to participate in regulation and control of the waterlogging tolerance of the rape. A molecular marker related to the waterlogging tolerance of the brassica napus is developed aiming at the BnaA06. ERF3 gene, and tests show that the waterlogging tolerance of the brassica napus can be detected by utilizing the molecular marker, so that varieties containing waterlogging tolerance genes are subjected to auxiliary selection and breeding, and the waterlogging tolerance breeding process of the brassica napus is accelerated.

Provided in the present invention are a Brassica napus drought-tolerant gene BnaC04ARL and the use thereof. The gene is expressed in a plant to improve drought tolerance of the plant. The gene comprises a nucleotide sequence selected from the group consisting of: a nucleotide sequence shown as SEQ ID NO: 1; and / or a nucleotide sequence encoding an amino acid sequence shown as SEQ ID NO: 2. The overexpression of the gene can improve the viability of Brassica napus under drought conditions, enhance root system development, and improve the thousand-grain weight, the grain yield per plant, the number of siliques per plant, dry weight per plant, silique length, etc.

The invention discloses a special growth regulator for pepper based on compounding of propionyl brassinolide and amino-oligosaccharin and application of the special growth regulator, and belongs to the technical field of agricultural biology. According to the regulating agent, 0.0008 to 0.0012 percent of propionyl brassinolide and 2.95 to 3.05 percent of amino-oligosaccharin are accurately matched with each other. The formula breaks through the contradiction between the root promotion requirement of high-dose amino-oligosaccharin and the safety threshold of low-dose brassinolide, and overcomes the phytotoxicity risk of the traditional dosage form. The regulator prepared by the invention can enhance the growth vigor of the pepper, improve the product and quality of the pepper, and also can effectively prevent viral diseases of the pepper.

The invention discloses a traditional Chinese medicine fermentation composition with a qi and blood nourishing function and a preparation method thereof, and belongs to the technical field of health care products. The traditional Chinese medicine fermented composition is prepared from the following raw materials in parts by weight: 15-20 parts of codonopsis pilosula, 12-18 parts of angelica sinensis, 5-10 parts of longan aril, 3-7 parts of purslane, 2-6 parts of brassica juncea, 4-9 parts of amomum tsao-ko and 5-11 parts of asparagus cochinchinensis. The traditional Chinese medicine fermentation composition is prepared through synergistic compatibility of various traditional Chinese medicines, an efficient extraction process and a multi-strain synergistic fermentation process, the qi and blood tonifying effect of the traditional Chinese medicine fermentation composition can be remarkably improved, and an innovative solution is provided for industrial production of qi and blood tonifying health care products.

The invention belongs to the technical field of plant molecular markers, and particularly discloses an Indel molecular marker related to the salt tolerance of brassica napus and application of the Indel molecular marker. The marker is located at the position of an A01 chromosome scafoldA0133077947 of a brassica napus reference genome ZS11.v10, and the genotype of a polymorphic site is CTAA or C. The invention further discloses a method for identifying the marker of the brassica napus. Large-scale group phenotypic association analysis proves that the molecular marker is remarkably associated with the salt tolerance of the brassica napus in the germination period, and when the polymorphic site genotype is CTAA, the salt tolerance in the germination period is high; when the genotype of the polymorphic site is C, low salt tolerance in the germination period is shown. The invention finds that the polymorphic site is obviously associated with the salt tolerance of the brassica napus for the first time, provides a powerful molecular tool for solving the problems of difficulty in phenotype identification, long period and low efficiency in salt tolerance breeding of the brassica napus, can accurately screen salt-tolerant materials in the early stage, and greatly accelerates the breeding process of new varieties of the salt-tolerant brassica napus.

The invention relates to the technical field of biological genetic engineering, in particular to application of a brassica napus BnaA01G0331000ZS gene in drought stress resistance. The BnaA01G0331000ZS gene is cloned by using cDNA of double 11 leaves as a template through a sequence published in a brassica napus genome database, an over-expression vector is constructed and genetically transformed into rape variety high oil for transgenic function verification, the drought resistance of the obtained over-expression transgenic line is obviously enhanced compared with that of a wild type, and the BnaA01G0331000ZS gene has the advantages that the BnaA01G0331000ZS gene is obtained; therefore, the gene can positively regulate the drought stress resistance of the rape. Therefore, the invention provides a new gene resource for adversity stress resistance of rape.

The invention relates to the technical field of crop planting, and provides a method for promoting germination of brassica napus seeds under low-temperature stress by using allantoin. The method comprises the following steps: firstly, disinfecting cabbage type rape seeds, and then soaking the cabbage type rape seeds in an allantoin solution with a specific concentration under room-temperature and weak-light conditions; the treated seeds do not need to be washed and are directly placed in a culture dish paved with double-layer filter paper, a proper amount of distilled water is added, and then the seeds are cultured in an incubator simulating a low-temperature environment. Experimental results show that the germination rate, the germination index and the vitality index of the brassica napus seeds are remarkably improved through allantoin treatment, and the average germination time is shortened. Mechanism research finds that allantoin improves the low-temperature resistance of the brassica napus by various ways of increasing the content of free water of seedlings, increasing the content of soluble sugar and proline, enhancing the activity of antioxidant enzyme, reducing active oxygen and malonaldehyde accumulation and the like. The method disclosed by the invention is low in cost and environment-friendly, and provides powerful guarantee for field production such as late direct seeding of the brassica napus.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a brassica napus BnaCP15A gene coded protein to improvement of plant salt tolerance. The technical problem to be solved by the invention is to provide a new choice for improving the salt tolerance of plants. The technical scheme of the invention is the application of the protein coded by the brassica napus BnaCP15A gene in improving the salt tolerance of the plant, wherein the amino acid sequence of the protein is shown as SEQ ID NO.2. The invention further discloses the application of the protein coded by the brassica napus BnaCP15A gene. The salt tolerance of the plant is improved by increasing the expression of the brassica napus BnaCP15A gene in the plant, and the nucleotide sequence of the BnaCP15A gene is as shown in SEQ ID NO. 1. The main purpose of the invention is to provide the application of the protein coded by the brassica napus BnaCP15A gene in improving the salt tolerance of the plant, a new choice is provided for improving the salt tolerance of the brassica napus, and the brassica napus BnaCP15A gene has important application value.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of a BnaWLT8 gene to improvement of waterflooding stress resistance of rape. The nucleotide sequence of the BnaWLT8 gene is as shown in SEQ ID NO. 1. The oilseed rape waterlogging resistance related gene BnaWLT8 provided by the invention provides an excellent gene resource and a new effective way for improving the waterlogging stress resistance of oilseed rape and cultivating a new variety of waterlogging-resistant brassica napus. Through transgenic genetic breeding, the expression level of the BnaWLT8 gene is enhanced, the waterlogging resistance and flooding resistance of the rape in the germination stage and the seedling stage can be remarkably enhanced, and the BnaWLT8 gene has wide application prospects and good social benefits in the aspects of stress resistance improvement of the rape and the like.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker for auxiliary selection of the leaf number of brassica napus, a primer group, a detection method and application, and relates to the technical field of biology and rape breeding. The SNP marker is located at the 9283404 bp position of a brassica napus A02 chromosome, a basic group of the site is a T allele or a C allele, and the T allele is associated with a leafy character. The SNP marker provided by the invention can solve the technical problem that the breeding efficiency of the high-photosynthetic-efficiency ideal-plant-type rape variety is restricted due to the fact that an effective molecular marker closely linked with the leaf number character of the brassica napus is lacked in the prior art and the character cannot be quickly and accurately screened in the early breeding stage.

A method for improving the germination rate and seedling growth vigor of scutellaria baicalensis seeds in a saline-alkali soil environment comprises the following steps: (1) preparing a seed soaking solution, namely preparing a quaternary plant growth regulator combined 6-BA + GA3 + NAA + brassin seed soaking solution; (2) preparing seed dressing mother liquor, namely preparing ternary seed dressing mother liquor of No.6 seed dressing mother liquor, planting elements and pyroligneous liquor; (3) seed soaking: soaking the seeds by using a quaternary seed soaking solution of 6-BA + GA3 + NAA + brassin; (4) airing; (5) seed dressing: carrying out secondary coating by using ternary seed dressing mother liquor of No. 6 seed dressing mother liquor, planting elements and pyroligneous liquor; and (6) sowing: sowing the seeds treated in the step (5) in a matrix containing 0.4% salt stress, and performing conventional culture. Compared with a traditional planting method, the germination rate and seedling growth vigor of the scutellaria baicalensis seeds treated through the method are greatly increased, the germination rate basically reaches 100%, the germination potential is 90%, and the effectiveness and advancement of the method are fully proved.

The invention discloses application of beta-glucosidase BnaBG27 in regulation and control of thousand seed weight of rape, and belongs to the technical field of gene engineering. According to the invention, the BGLU27 gene is separated and obtained from brassica napus Westar, and the gene is found to be highly expressed in rape seeds. Experiments find for the first time that the BGLU27 gene positively regulates the thousand seed weight of the rape, and the thousand seed weight of the rape can be increased by utilizing the gene, so that high-yield rape germplasm can be obtained. The method has a very important application prospect in breeding and improvement of high-yield rape varieties.

The invention provides an application of a phosphatidylinositol monophosphate 5-kinase gene BnPIP5K9 of rape in regulating and controlling the oil content of the rape. The genetic basis of the oil content of rape seeds is analyzed on the basis of multiple omics, and it is found that the expression quantity of the phosphatidylinositol monophosphate 5-kinase gene BnPIP5K9 in rape is significantly positively correlated with the oil content; the gene BnPIP5K9 is subjected to gene overexpression and knockout to create a mutant for functional verification by further utilizing a tobacco mosaic virus double-35S promoter and a CRISPR / Cas9 gene editing technology, the result shows that the oil content of rape seeds can be remarkably increased by 2.16-2.77% by overexpression of the gene, in addition, rape germplasm resources with gene BnPIP5K9 function deficiency can be obtained through the CRISPR / Cas9 technology, and the rape seed quality is improved. A new theory and a new gene resource are provided for high-oil breeding and oil improvement of the rape, and the method has extremely high application value and potential.

The invention belongs to the technical field of agricultural planting, and particularly relates to application of brassinolide in improving volatile aroma substances of chilies. The invention further provides a planting method for improving the volatile aroma substances of the chilies. The planting method comprises the following steps: dissolving brassinolide in water to obtain a brassinolide solution; the brassinolide solution is used for spraying pepper seedlings in different growth periods until the leaves slightly drip water. According to the method, in the growth and development process of pepper fruits, the brassinolide aqueous solution is sprayed to pepper plants for treatment, the content of volatile aroma substances of the peppers can be greatly increased, the commodity of the peppers is increased, and economic benefits are increased for farmers. According to the method, the improvement effect can be achieved only through a foliage spraying method, operation is easy and convenient, the using amount is small, and the effect taking speed is high. As a plant regulator substance, brassinolide has the advantages of quick effect on plants, low dosage, quick metabolism, no plant regulator residue, safety, reliability and wide application prospect.

This invention relates to the field of agricultural biotechnology, specifically to... BnaC04.bZIP16 Application of genes in improving plant salt tolerance. This invention provides... BnaC04.bZIP16 The gene, whose sequence is shown in SEQ ID NO.1, and whose encoded protein sequence is shown in SEQ ID NO.2, is found in this invention to be highly expressed in wild-type Brassica napus after irrigation with salt water. BnaC04.bZIP16 Genes, and transgenic rapeseed overexpressing this gene, can grow better under salt stress conditions; knockout gene... BnaC04.bZIP16 The genetically modified rapeseed is more difficult to grow in salt-stressed environments, thus verifying... BnaC04.bZIP16 This gene plays a positive regulatory role in enhancing the salt tolerance of rapeseed and provides a key molecular tool for developing new rapeseed varieties with high salt tolerance. This gene has broad application potential and commercial value in the field of agricultural planting, especially in improving the salt tolerance of crops and breeding highly salt-tolerant transgenic crops.

The invention relates to the field of agricultural bioengineering, in particular to application of an oilseed rape BnaA08G0167600WE gene in enhancing pod shattering resistance of oilseed rape. According to the invention, after a transgenic plant is constructed in oilseed rape by utilizing a modern biotechnological means and the oilseed rape gene BnaA08G0167600WE is knocked out, the silique of the mutant is not easy to crack compared with a wild type under the action of external force, and a separation layer and a wood layer of the cross section of the silique of the mutant have more cell connections compared with the wild type, so that the yield of the mutant is improved. And the generation of the mutants provides an important genetic material for further researching the function of the gene and the effect of the gene in the rape.

The invention relates to the field of genes, in particular to molecular markers for judging flowering time of brassica napus L. The molecular markers are Indel-282, Indel-326 and Indel-353 respectively and are located on an A02 chromosome of the brassica napus L.. The molecular marker has high specificity and stability, and is significantly related to the flowering time. The markers show high specificity and stability in brassica napus plants under different genetic backgrounds. By detecting the molecular markers through PCR amplification and electrophoresis, the flowering time of different plants can be quickly and accurately identified in the early stage of breeding. Therefore, the flowering time of the plant can be determined in the early stage, so that the breeding efficiency is improved. The molecular markers can be used for molecular marker-assisted selective breeding, and plants with early flowering or late flowering characteristics can be quickly screened by detecting the markers, so that the breeding process of excellent varieties is accelerated, and the functions and regulation mechanisms of related genes are further disclosed.

The invention belongs to the technical field of plant breeding, and particularly relates to a breeding method of high-yield multi-chamber brassica napus. Comprising the following steps: performing hybridization by taking a mustard type rape multi-chamber resource as a female parent and a cabbage type rape as a male parent to obtain an F1 generation, and performing isolated propagation to obtain F2 free seeds; selecting partial brassica napus type single plants from F2 free seeds for selfing propagation to obtain F3-generation seeds, planting the F3-generation seeds according to plant lines, performing selfing propagation, screening out single-plant selfing seeds with multi-chamber pods, continuing selfing, continuously repeating for 2-4 generations, screening out single plants closest to male parent characters, and obtaining high-yield multi-chamber brassica napus resources. The multi-chamber brassica napus resource with the multi-chamber pod rate larger than 60% is obtained through the method, the novel multi-chamber brassica napus resource with the pod length close to that of double No.11 in brassica napus parents is obtained, and the pod length of the novel multi-chamber brassica napus resource reaches 11.01 cm.

The invention discloses a special-effect foliar fertilizer for improving cold resistance of durian seedlings as well as a preparation method and application of the special-effect foliar fertilizer, and belongs to the technical field of durian planting. The special-effect foliar fertilizer prepared by the invention is prepared from the following raw materials in parts by weight: 2 to 5 parts of fulvic acid, 10 to 20 parts of antifreeze agent, 0.5 to 2 parts of pine needle extract, 3 to 8 parts of alginate oligosaccharide, 0.3 to 0.8 part of 14-hydroxybrassinosterol, 0.2 to 0.5 part of 0.01 to 0.1 percent v / v polysorbate-20, 2 to 4 parts of chitosan, 1 to 3 parts of trehalose, 0.1 to 1 part of brassinolide, 0.1 to 0.3 part of salicylic acid and 6 to 10 parts of glycerol. The special-effect foliar fertilizer is simple to prepare, safe overwintering of durian seedlings at the low temperature of 4-15 DEG C can be effectively guaranteed, and the problems of dead seedlings and runt seedlings caused by low temperature are remarkably solved. The durian seedling culture medium is safe and environment-friendly in components, is beneficial to reduction of environmental pollution and pesticide residues, provides rich nutrition for breeding and growth of durian seedlings, and has remarkable application potential for promoting development of the durian industry.

The invention discloses a molecular marker of a brassica napus salt-tolerant gene BnaA07.GRP3 and application of the molecular marker, and belongs to the technical field of rape breeding. According to the invention, two haplotypes hap0 and hap1 of the salt-tolerant gene BnaA07. GRP3 are found for the first time, a corresponding molecular marker is provided for the optimal haplotype hap1 of the BnaA07. GRP3, and the optimal haplotype hap1 of the BnaA07. GRP3 is specifically amplified, so that the salt-tolerant material of the brassica napus is identified. The salt-resistant material of the brassica napus can be quickly and accurately identified, and the repeatability is good. The method is applied to auxiliary selective breeding of salt-resistant characters of the brassica napus, the breeding efficiency of the salt-resistant brassica napus can be greatly improved, the selection range of salt-resistant materials is expanded, the method has huge application potential and prospects, and an ideal way is provided for cultivating the salt-resistant brassica napus.

The invention provides application of a BnaXPT gene in regulating and controlling the content of oil and protein in rape seeds. According to the invention, the key gene XPT gene for regulating and controlling the balance of the oil content and the protein content of the rape seeds is excavated on the whole genome level through multi-omics association analysis, and by constructing an XPT overexpression strain and an XPT function-deficient rape variety, it is found that the protein content of the rape seeds can be remarkably improved through overexpression of the gene; by mutating the gene, the oil content of rape seeds can be remarkably increased, a brand new target is provided for improving and optimizing the content and balance of the two nutrient substances of the rape, a new way and means are provided for creating the rape with high oil content or high protein content, and the application prospect is wide.

The invention relates to a haploid induction method based on a brassica napus phospholipase gene BnaPLA2-a and application of the haploid induction method, the BnaPLA2-a gene in brassica napus is knocked out through a gene editing technology, a mutant material capable of inducing haploid generation is obtained, and the application blank of a phospholipase pathway in dicotyledon haploid induction is filled. Compared with a traditional microspore culture technology, the invention provides a brand-new haploid induction path which is derived from the rape and is used for the rape. The invention provides a brand new technical tool and germplasm resources for genetic breeding of brassica napus. Meanwhile, the invention discloses a gene, a mutant, a creation method and application in breeding.

The invention belongs to the field of plant biotechnology and plant molecular breeding, and particularly relates to a brassica napus BnabZIP1 gene and application thereof in prevention and control of sclerotinia rot of colza. According to the invention, a gene editing technical vector is constructed aiming at sgRNA of the BnabZIP1 gene, agrobacterium tumefaciens is transformed, then brassica napus is transfected, and a homozygous mutant plant after the BnabZIP1 gene is knocked out has stronger sclerotiniose resistance. Results show that the BnabZIP1 gene can be used as a molecular marker or a genetic engineering improvement target of sclerotiniose resistance of rape, is used for screening and creating disease-resistant rape varieties, and has a good molecular breeding application prospect.

The invention discloses a BcC3H33 gene of flowering cabbage and an application of a protein encoded by the BcC3H33 gene in resisting cadmium stress. The invention provides a new application of the BcC3H33 gene of the flowering cabbage and the encoded protein thereof in regulation and control of growth and development of the flowering cabbage and cadmium stress response, and research shows that the BcC3H33 gene has very strong response to cadmium stress in roots and leaves of the flowering cabbage and can regulate and control the growth and development of the flowering cabbage and promote the growth of the flowering cabbage; after the BcC3H33 gene is over-expressed in the flowering cabbage, the biological accumulation amount of the flowering cabbage is increased, the morphological index is improved, the photosynthetic ability is enhanced, the stress resistance is enhanced, the influence of cadmium stress on the growth and development of the flowering cabbage can be relieved, and the growth of the flowering cabbage can also be promoted under the cadmium stress. Therefore, the BcC3H33 gene can improve the cadmium stress tolerance of the Chinese flowering cabbage, improve the crop adaptability, provide a basis and a method for stress-resistant breeding of the Chinese flowering cabbage, provide key gene resources for the Chinese flowering cabbage and other crops, and meet different cultivation environment requirements.

The invention discloses a KASP molecular marker related to the grain weight of brassica napus, a primer and application. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO.1, and A / G mutation exists at the 51st basic group. A KASP primer for detecting the molecular marker comprises nucleotide sequences as shown in SEQ ID NO. 2, SEQ ID NO. 3 and SEQ ID NO. 4. The KASP molecular marker developed by the invention can accurately distinguish large-grain varieties and small-grain varieties of the brassica napus, provides a convenient and reliable-result molecular marker for detecting the brassica napus with high thousand grain weight, can realize screening of materials with different grain weights of the brassica napus in the early stage, has good application value, and can be used for detecting the brassica napus with high thousand grain weight. The method can be widely applied to the field of brassica napus breeding and shortens the breeding process.

The invention relates to the technical field of brassica napobrassica, in particular to a method for slightly fermenting brassica napobrassica, which comprises the following steps: performing primary fermentation by using a complex microbial inoculant, and then performing secondary fermentation at low temperature by using a Weissella fusiformis microbial inoculant. The method can shorten the fermentation period and improve the production efficiency.

The invention belongs to the technical field of crop molecular markers, and particularly discloses a molecular marker for identifying the salt tolerance of brassica napus in the germination period and application of the molecular marker. The molecular marker is located at the position of an A02 chromosome scafoldA028707654 of a brassica napus reference genome ZS11.v10, and the allelotype of the molecular marker is wild type C or mutant type CATATT insertion. The invention also provides a specific primer group for detecting the molecular marker, and the salt tolerance (sensitive type or tolerance type) of the brassica napus line can be quickly identified according to the existence of 411bp and 416bp bands through double PCR amplification and electrophoresis detection. The molecular marker is significantly associated with salt tolerance phenotypes, the detection method is rapid, accurate and low in cost, the molecular marker can be used for brassica napus salt tolerance germplasm resource screening and molecular marker-assisted breeding, and the breeding efficiency is significantly improved.

The invention belongs to the field of rape breeding, and particularly discloses a sclerotinia rot resistance related gene BnaCLE20 of rape and application of the sclerotinia rot resistance related gene BnaCLE20 in rape breeding. The sequence of the BnaCLE20 gene is as shown in SEQ ID NO. 1, and the coded protein is as shown in SEQ ID NO. 2; the BnaCLE20 gene of the brassica napus is identified to be capable of regulating the sclerotinia rot resistance of the brassica napus for the first time, a molecular network for regulating the sclerotinia rot resistance of plants is enriched, and the BnaCLE20 gene plays an important role in researching a molecular mechanism formed by the disease resistance of the plants. The overexpression of the BnaCLE20 gene disclosed by the invention can reduce the content of active oxygen, malondialdehyde and oxalic acid in rape leaves infected by sclerotinia sclerotiorum; meanwhile, the activity of catalase in the rape leaves infected by sclerotinia sclerotiorum is enhanced; the sclerotiniose resistance of the oilseed rape is remarkably improved, and the method has important significance in guaranteeing the high and stable yield of the oilseed rape by improving the sclerotiniose resistance of the oilseed rape.