208 results about "Brassica" patented technology

Provided in the present invention are a Brassica napus drought-tolerant gene BnaC04ARL and the use thereof. The gene is expressed in a plant to improve drought tolerance of the plant. The gene comprises a nucleotide sequence selected from the group consisting of: a nucleotide sequence shown as SEQ ID NO: 1; and / or a nucleotide sequence encoding an amino acid sequence shown as SEQ ID NO: 2. The overexpression of the gene can improve the viability of Brassica napus under drought conditions, enhance root system development, and improve the thousand-grain weight, the grain yield per plant, the number of siliques per plant, dry weight per plant, silique length, etc.

The invention belongs to the technical field of plant molecular markers, and particularly discloses an Indel molecular marker related to the salt tolerance of brassica napus and application of the Indel molecular marker. The marker is located at the position of an A01 chromosome scafoldA0133077947 of a brassica napus reference genome ZS11.v10, and the genotype of a polymorphic site is CTAA or C. The invention further discloses a method for identifying the marker of the brassica napus. Large-scale group phenotypic association analysis proves that the molecular marker is remarkably associated with the salt tolerance of the brassica napus in the germination period, and when the polymorphic site genotype is CTAA, the salt tolerance in the germination period is high; when the genotype of the polymorphic site is C, low salt tolerance in the germination period is shown. The invention finds that the polymorphic site is obviously associated with the salt tolerance of the brassica napus for the first time, provides a powerful molecular tool for solving the problems of difficulty in phenotype identification, long period and low efficiency in salt tolerance breeding of the brassica napus, can accurately screen salt-tolerant materials in the early stage, and greatly accelerates the breeding process of new varieties of the salt-tolerant brassica napus.

The invention relates to the field of agricultural bioengineering, in particular to application of an oilseed rape BnaA08G0167600WE gene in enhancing pod shattering resistance of oilseed rape. According to the invention, after a transgenic plant is constructed in oilseed rape by utilizing a modern biotechnological means and the oilseed rape gene BnaA08G0167600WE is knocked out, the silique of the mutant is not easy to crack compared with a wild type under the action of external force, and a separation layer and a wood layer of the cross section of the silique of the mutant have more cell connections compared with the wild type, so that the yield of the mutant is improved. And the generation of the mutants provides an important genetic material for further researching the function of the gene and the effect of the gene in the rape.

The invention relates to the field of genes, in particular to molecular markers for judging flowering time of brassica napus L. The molecular markers are Indel-282, Indel-326 and Indel-353 respectively and are located on an A02 chromosome of the brassica napus L.. The molecular marker has high specificity and stability, and is significantly related to the flowering time. The markers show high specificity and stability in brassica napus plants under different genetic backgrounds. By detecting the molecular markers through PCR amplification and electrophoresis, the flowering time of different plants can be quickly and accurately identified in the early stage of breeding. Therefore, the flowering time of the plant can be determined in the early stage, so that the breeding efficiency is improved. The molecular markers can be used for molecular marker-assisted selective breeding, and plants with early flowering or late flowering characteristics can be quickly screened by detecting the markers, so that the breeding process of excellent varieties is accelerated, and the functions and regulation mechanisms of related genes are further disclosed.

The invention belongs to the technical field of plant breeding, and particularly relates to a breeding method of high-yield multi-chamber brassica napus. Comprising the following steps: performing hybridization by taking a mustard type rape multi-chamber resource as a female parent and a cabbage type rape as a male parent to obtain an F1 generation, and performing isolated propagation to obtain F2 free seeds; selecting partial brassica napus type single plants from F2 free seeds for selfing propagation to obtain F3-generation seeds, planting the F3-generation seeds according to plant lines, performing selfing propagation, screening out single-plant selfing seeds with multi-chamber pods, continuing selfing, continuously repeating for 2-4 generations, screening out single plants closest to male parent characters, and obtaining high-yield multi-chamber brassica napus resources. The multi-chamber brassica napus resource with the multi-chamber pod rate larger than 60% is obtained through the method, the novel multi-chamber brassica napus resource with the pod length close to that of double No.11 in brassica napus parents is obtained, and the pod length of the novel multi-chamber brassica napus resource reaches 11.01 cm.

The invention relates to a haploid induction method based on a brassica napus phospholipase gene BnaPLA2-a and application of the haploid induction method, the BnaPLA2-a gene in brassica napus is knocked out through a gene editing technology, a mutant material capable of inducing haploid generation is obtained, and the application blank of a phospholipase pathway in dicotyledon haploid induction is filled. Compared with a traditional microspore culture technology, the invention provides a brand-new haploid induction path which is derived from the rape and is used for the rape. The invention provides a brand new technical tool and germplasm resources for genetic breeding of brassica napus. Meanwhile, the invention discloses a gene, a mutant, a creation method and application in breeding.

The invention belongs to the technical field of crop molecular markers, and particularly discloses a molecular marker for identifying the salt tolerance of brassica napus in the germination period and application of the molecular marker. The molecular marker is located at the position of an A02 chromosome scafoldA028707654 of a brassica napus reference genome ZS11.v10, and the allelotype of the molecular marker is wild type C or mutant type CATATT insertion. The invention also provides a specific primer group for detecting the molecular marker, and the salt tolerance (sensitive type or tolerance type) of the brassica napus line can be quickly identified according to the existence of 411bp and 416bp bands through double PCR amplification and electrophoresis detection. The molecular marker is significantly associated with salt tolerance phenotypes, the detection method is rapid, accurate and low in cost, the molecular marker can be used for brassica napus salt tolerance germplasm resource screening and molecular marker-assisted breeding, and the breeding efficiency is significantly improved.

The invention belongs to the technical field of molecular marker development and molecular marker assisted breeding, and particularly relates to a brassica napus salt tolerance related KASP molecular marker and application thereof. The invention discloses novel salt-tolerant allelic mutation of major QTL (quantitative trait loci) of A06 chromosome and C08 chromosome of brassica napus. A molecular marker is obtained through whole genome association analysis. According to the brassica napus salt tolerance related KASP molecular marker disclosed by the invention, the amplification effect of marking SNPA06.1, SNPA06.2 and SNPC08 is optimal, and the SNP variation of salt-tolerant germplasm and sensitive germplasm can be obviously distinguished.

This invention discloses the application of the BnaTOE1 gene in regulating the flowering time of Brassica napus and its molecular marker primers for identification. The DNA nucleotide sequence of the BnaTOE1 gene is shown in SEQ ID NO.1, with a sequence length of 1254 bp, and the encoded amino acid sequence is shown in SEQ ID NO.2. The molecular marker primers used to identify the flowering time of Brassica napus are shown in SEQ ID NO.15 and SEQ ID NO.16. Functional analysis of genetic transformation in Brassica napus shows that BnaTOE1 participates in regulating the flowering time of Brassica napus. The development of molecular markers is of great significance for promoting the genetic improvement and breeding of Brassica napus.

This invention discloses a rapeseed BnaALaAT1 The application of genes in breeding stress-resistant, high-yielding plant varieties, the aforementioned BnaALaAT1 The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention clones the versatile gene from rapeseed. BnaALaAT1 We constructed pure-line plants by introducing rapeseed and conducted phenotypic investigations under various conditions. The experimental results showed that... BnaALaAT1 Genetically modified rapeseed exhibits significantly superior growth compared to the wild type under both normal and various adverse conditions, fully demonstrating that this gene can enhance the plant's survival ability under various adverse conditions by regulating the plant's response mechanism to multiple stresses, providing a crucial guarantee for the stable growth of plants under diverse adverse conditions. Compared to the wild type, transgenic plants not only show a significant increase in yield, with a maximum increase of 53.7%, but also a significant increase in oil content.

The present application relates to the technical field of plant genetic breeding, and particularly relates to a Brassica campestris fatty acyl reductase BcFAR2 gene, an expression protein thereof and application, a base sequence of the Brassica campestris fatty acyl reductase BcFAR2 gene is shown as SEQ ID NO. 1, and an amino acid sequence of the expression protein is shown as SEQ ID NO. 2, the Brassica campestris fatty acyl reductase BcFAR2 gene is cloned, and BcFAR2 gene overexpression Brassica campestris sterile plants can restore pollen activity; gene knockout Brassica campestris fertile plants can significantly reduce pollen activity; in Arabidopsis thaliana, overexpression of the gene can significantly improve salt tolerance of the transgenic plants; and in Brassica campestris, overexpression of the gene can significantly enhance salt tolerance.

The invention relates to the technical field of bioinformatics and crop breeding, and particularly discloses a method, a device and equipment for predicting the close planting yield of brassica napus by using hypocotyl length based on a whole genome selection technology. The method is characterized by comprising the following steps: acquiring whole genome sequencing data of brassica napus germplasm; inputting the whole genome sequencing data into a target model; outputting hypocotyl length data of the brassica napus under a close planting condition through the target model; and according to the hypocotyl length data of the brassica napus under the close planting condition, obtaining a brassica napus germplasm yield potential sorting result. According to the method, whole genome selection and key morphological characters (hypocotyl length) under specific stress (close planting conditions) are combined for the first time, high-yield varieties suitable for close planting cultivation can be efficiently and accurately screened out without on-site close planting tests in the early stage of rape breeding, and the breeding efficiency is remarkably improved.

The invention discloses a dCAPS molecular marker primer linked with a brassica napus saline-alkaline tolerance gene BnaC03.RBGB3 and application of the dCAPS molecular marker primer. The primer comprises a forward primer and a reverse primer, and through PCR amplification and specific restriction enzyme digestion treatment, two genotypes of high resistance and high sensitivity of brassica napus saline-alkaline tolerance can be distinguished on agarose gel electrophoresis; the high-resistance genotype is represented by two electrophoretic bands (195bp and 23bp), and the high-sensitivity genotype is a band which cannot be digested by enzyme. The method disclosed by the invention is simple and convenient to operate and reliable in result, can be used for rapidly screening and identifying the saline-alkaline-tolerant variety of the brassica napus, and provides a powerful tool for breeding the saline-alkaline-tolerant variety of the brassica napus. By implementing the technology, not only is the breeding efficiency improved, but also a new thought and strategy are provided for molecular marker-assisted breeding of saline-alkaline tolerance of crops, and development and application of a saline-alkaline tolerance breeding technology of brassica napus and even other crops are promoted.

The present application belongs to the field of plant genetic engineering and biotechnology, and particularly relates to a method for creating a dwarf material of Brassica napus by using CRISPR / Cas9 gene editing technology and application. The method edits the Brassica napus BnaA06G0083400WE gene by using CRISPR / Cas9 gene editing technology to obtain a dwarf material of Brassica napus; the nucleotide sequence of the coding gene mutant of the Brassica napus dwarf material is SEQ ID NO. 1. The present application edits the Brassica napus BnaA06G0083400WE gene by using CRISPR / Cas9 gene editing technology to obtain a dwarf Brassica napus, which provides valuable genetic resources and germplasm resources for Brassica napus breeding; the method has strong characteristics and is easy to obtain, is an effective way to realize the improvement of target traits and cultivate new materials, and can be applied to Camellia sinensis breeding.

The invention discloses a KASP molecular marker primer group for identifying saline-alkaline tolerance of rape and application of the KASP molecular marker primer group, and belongs to the technical field of molecular marker-assisted breeding. Comprising a first primer (SEQ ID NO.2), a second primer (SEQ ID NO.3) and a universal primer (SEQ ID NO.4), the primer group is designed based on a key SNP site (A / T) of a BnaA02. CDKE1 gene, and rapid and accurate typing of rape genotypes can be realized through a KASP technology. The invention also provides a kit containing the primer group, an application of the kit in saline-alkaline resistance identification of rape, and a corresponding identification method. The method has the advantages of simplicity and convenience in operation, stable result, high flux, low cost and the like, and is suitable for large-scale auxiliary breeding and molecular identification of saline-alkaline tolerant rape varieties.

The invention provides a planting method for promoting early maturing and high yield of spring wheat in Ningxia irrigated areas, and belongs to the technical field of crop regulation and control. The method comprises the following steps: soaking seeds with a seed soaking agent, and then early sowing from February 15th to February 25th; the seed soaking agent is prepared from 5 to 10g / L of calcium chloride, 2 to 3g / L of alginate oligosaccharide, 100 to 200mg / L of proline and 0.05 to 0.12 mg / L of brassinolide. Through the synergistic effect of the calcium chloride, the alginate oligosaccharide, the proline and the brassinolide, the germination rate of the early sowing spring wheat seeds at the low temperature of 5 DEG C is increased to 85% or above, the problem of poor spring wheat seedling emergence caused by early sowing low temperature is solved, and the purpose of early maturing without yield reduction is achieved.

This invention belongs to the field of organic agriculture, specifically a biological agent for promoting wheat photosynthesis and its preparation method. Addressing the problems of existing biological agents having relatively simple components, low absorption and utilization efficiency by wheat ears, and a tendency to reduce nitrate reductase activity, nitrogen utilization, and chlorophyll content in wheat leaves, the following solution is proposed: the biological agent comprises the following raw materials in parts by weight: 50-65 parts of yield-enhancing amine, 15-20 parts of choline chloride, 10-15 parts of diethylaminoethanol hexanoate, 5-10 parts of brassinolide, 3-5 parts of p-chlorophenoxyacetic acid, 1-3 parts of calcium diphenylureasulfonate, 2-5 parts of trace agents, and 2-4 parts of ethylene glycol styraldehyde. The biological agent of this invention can promote better photosynthesis in wheat, enhance nitrate reductase activity, and improve nitrogen utilization.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a transcription factor BnC05.PLATZ01 gene for regulating and controlling sclerotinia rot resistance of rape and application of the transcription factor BnC05.PLATZ01 gene. A transcription factor BnC05.PLATZ01 gene capable of enhancing sclerotiniose resistance of rape is screened, and the nucleotide sequence of the transcription factor BnC05.PLATZ01 gene is as shown in SEQ ID NO: 1; the sequence of the protein coded by the gene is as shown in SEQ ID NO: 2. According to the method, a pGWB418-BnC05. PLATZ01 overexpression strain is obtained by virtue of agrobacterium tumefaciens mediated transformation. Sclerotinia sclerotiorum inoculation identification on a transgenic material finds that the sclerotinia rot resistance of a BnC05. PLATZ01 overexpression strain is enhanced, which indicates that the BnC05. PLATZ01 is a positive regulation factor of the sclerotinia rot resistance of the rape and has a positive regulation effect in the sclerotinia rot resistance of the rape, and the sclerotinia rot resistance of the rape can be remarkably improved by overexpressing the gene.

This invention discloses a stress-resistant and yield-increasing plant growth regulator, its preparation method, and its application, belonging to the field of plant growth regulators. The stress-resistant and yield-increasing plant growth regulator of this invention comprises the following raw materials: *Suaeda salsa* extract, a stress-resistant biostimulant, propionyl brassinolide, styracil, polyaspartic acid, γ-aminobutyric acid, emulsifier, penetrant, co-emulsifier, pH buffer, and deionized water; wherein the stress-resistant biostimulant is perilla lactone. This invention significantly improves the tolerance of wheat to salt-alkali stress by strengthening the antioxidant system and osmotic regulation capacity, ensuring its basic survival and normal physiological functions; and effectively reverses stress-induced growth inhibition through mechanisms such as promoting root development, enhancing photosynthesis, and driving product translocation to grains, thereby achieving a significant yield increase under adverse conditions.

The application discloses a high-efficiency genetic transformation and gene editing method for cabbage, and belongs to the technical field of biological breeding, and comprises the following steps: (1) introducing a CRISPR / Cas9 gene editing vector into a callus of a target cabbage material; (2) screening the cabbage callus into which the CRISPR / Cas9 gene editing vector is successfully introduced, and subjecting the cabbage callus to regeneration culture; and (3) screening a T0 generation transgenic cabbage in which gene editing occurs; wherein the CRISPR / Cas9 gene editing vector contains sgRNA of a target editing gene, and a PAM sequence of the CRISPR / Cas9 gene editing vector is 5'-'NGGT'-3', wherein N is one of A, C and G. The method can significantly improve gene editing efficiency and types.

The invention provides a cabbage head harvester with a variable-diameter leaf removing mechanism and a harvesting method. The problems that in the prior art, manual harvesting efficiency is low, and leaf removing cannot be adjusted in a self-adaptive mode according to crop sizes and irregular shapes in equipment harvesting are solved. Comprising a movable chassis, a leaf threshing and root cutting mechanism, a leaf removing mechanism and a material transferring mechanism, the movable chassis comprises a rack, and a middle through groove is formed in the front-back direction of the rack; the leaf threshing and root cutting mechanism comprises a leaf threshing assembly and a root cutting assembly, and the leaf threshing assembly and the root cutting assembly are connected into the middle barrel groove of the rack through the conveying mechanism. The leaf threshing assembly is located in front of the root cutting assembly. The leaf removing mechanism is located below the output end of the conveying mechanism. The material transferring mechanism comprises a material transferring plate and a material transferring assembly. When the material transferring assembly acts, the leaf-removed cabbage heads output from an outlet of the material transferring plate are conveyed into a material storage pool above the machine frame. The machine can not only improve the harvesting efficiency, but also adapt to green vegetable heads with different sizes and irregular shapes for leaf removal.

The invention belongs to the technical field of gene engineering, and particularly relates to an application of a BnaA02g04730D gene in improving the salt tolerance of oilseed rape. According to the invention, a high-salt-tolerance germplasm 'Xiangong saline-alkali oil No.1' is obtained by screening, and a key candidate gene BnaA02g04730D responding to salt stress is excavated by combining absolute quantitative transcriptomics analysis. Yeast stress phenotype identification and transgenic rape verification both prove that the gene positively regulates the salt tolerance of rape. Functional verification shows that under the stress of 0.24% NaCl, the SOD activity, the CAT activity and the POD activity of the overexpressed plant are remarkably improved compared with those of a wild type, and the MDA content is remarkably reduced. The invention preliminarily illustrates a molecular mechanism for endowing rape with salt tolerance by regulating and controlling an anti-oxidation defense system and a hormone signal channel. A new perspective is provided for analysis of a salt-tolerant molecular mechanism of the rape, and a gene resource with application value is also provided for molecular breeding of a new salt-tolerant variety.

The invention relates to the field of agricultural biochemical engineering and plant protection, and particularly discloses a liquid film-forming agent for preventing crops from freezing injury due to late spring coldness and a preparation method of the liquid film-forming agent. The film-forming agent is prepared from carboxymethyl chitosan, sodium carboxymethyl cellulose, kasugamycin, brassinolide, glycol and glycerol mixed dihydric alcohol, Tween 80 and deionized water according to a specific proportion. The preparation method comprises the key processes of step-by-step dissolution, mixing, functional component addition and the like. Organic fusion of physical barrier, ice nucleus inactivation and physiological regulation is realized through multiple action mechanisms of natural polymer composite film forming, biopesticide targeted sterilization, plant hormone induced cold resistance and dihydric alcohol synergistic antifreezing. The product can provide efficient protection at a low temperature of-4 DEG C, significantly reduces the frost damage rate, has the characteristic of 28-day natural rapid degradation, and solves the technical bottlenecks of single function, difficult degradation and poor environmental compatibility of a traditional film agent.

The invention relates to a brassica napus drought-resistant cultivation method based on a target gene, which comprises the following steps: S1, cloning a BnaWRKY038 gene, and the nucleotide sequence of the BnaWRKY038 gene is as shown in SEQ ID NO: 1; s2, constructing an overexpression vector of the BnaWRKY038 gene, and transforming agrobacterium tumefaciens; s3, performing mediation transformation on the hypocotyl of the brassica napus by utilizing the agrobacterium to obtain a transgenic plant; s4, screening and identifying the transgenic plants to obtain the drought-resistant brassica napus with the overexpressed BnaWRKY038 gene. By obtaining the BnaWRKY038 overexpressed transgenic plants and based on phenotype results of the overexpressed transgenic rape plants, the drought resistance of the plants can be positively regulated under drought treatment of the rapes, a new choice is provided for screening dominant stress-resistant genes, and the drought-resistant brassica napus with the overexpressed BnaWRKY038 gene is obtained. The method has important application value in the field of plant genetic engineering, can effectively reduce economic loss caused by drought, and promotes cultivation of new varieties of drought-resistant rape.

The invention relates to a bagging-free quality-improving and efficiency-improving pesticide for reducing the insect fruit rate of pomes and a spraying method of the bagging-free quality-improving and efficiency-improving pesticide. The quality-improving and efficiency-improving pesticide comprises the following components: 1-100 g / L of pullulan; 0.5 g / L to 2 g / L of EDTA-2NaCa; the nutrient solution is prepared from 0.3 g / L to 5 g / L of L-calcium aspartate, 1 g / L to 3 g / L of D-calcium gluconate, 2 g / L to 4 g / L of sucrose fatty acid ester, 5 g / L to 15 g / L of tea polyphenol, 2 g / L to 6 g / L of gallic acid, 0.5 mg / L to 1.5 mg / L of brassinolide, 2 g / L to 4 g / L of fumed silica, 0.1 g / L to 0.3 g / L of xanthan gum, 1 g / L to 3 g / L of D-sorbitol, 0.1 g / L to 0.5 g / L of an anti-coagulant and water serving as a solvent. After the pesticide is sprayed, the pest prevention and control effect is equal to that of bagging, the cost is reduced by 68%, sunburn and bitter pox diseases are reduced, the chlorophyll SPAD value is increased by 22%, the photosynthetic rate is increased by 18%, and high yield is promoted.

This invention belongs to the field of grape cultivation technology, specifically relating to a fruit enlargement agent for Daqing grapes, its preparation method, and its application. The fruit enlargement agent for Daqing grapes is prepared from an aqueous solution of gibberellin, thiabendazole, and brassinolide. Specifically, the concentration of gibberellin in the fruit enlargement agent is 20 mg / L~25 mg / L, the concentration of thiabendazole is 1.0 mg / L~1.25 mg / L, and the concentration of brassinolide is 0.025 μg / L~0.050 μg / L. The fruit enlargement agent of this invention, combined with fruit thinning treatment, can increase the weight of a single Daqing grape berry by at least 20% while retaining the soluble solids content of the grape.

The application belongs to the field of plant biotechnology and plant molecular breeding, and particularly relates to Brassica napus BnabZIP1 Gene and application thereof in prevention and control of oilseed rape sclerotinia stem rot BnabZIP1 The sgRNA of the gene is used to construct a gene editing vector, and the agrobacterium is transformed, then the Brassica napus is transfected, and it is found that BnabZIP1 The homozygous mutant plant after the gene knockout has stronger resistance to the sclerotinia stem rot. BnabZIP1 The gene can be used as a molecular marker or a genetic engineering improvement target for the resistance to the sclerotinia stem rot, is used for screening and creating the disease-resistant oilseed rape varieties, and has a good molecular breeding application prospect.

The invention provides fertility restorer Brassica napus plants, plant material and seeds, characterized in that these products harbor a specific introgression fragment of the Ogura fertility restorer at the end of chromosome N 10. Tools are also provided which allow detection of the fertility restorer.

The invention relates to a plant cultivation method, in particular to a sterile miniature cuttage rapid propagation method for brassica napobrassica. According to the method, stem segments with buds or terminal buds of the brassica napobrassica are used as explants for asexual propagation, budlet micro cutting is performed in a sterile environment, plantlets with roots are directly induced without adding any plant hormones and inducing cluster buds and calluses, and rapid propagation of the brassica napobrassica is realized through continuous micro cutting. The method is not limited by seasons and propagation materials, seedlings can be produced in a manual control environment all the year round, the propagation speed is high, and the coefficient is large.

The invention relates to a biogas slurry composite biological agent for improving grape quality and an application method thereof, and belongs to the technical field of grape planting. Aiming at the problems that the direct application effect of the existing biogas slurry is unstable, the nutrients are unbalanced, and the traditional chemical fertilizer is easy to cause soil hardening and fruit quality reduction, the invention provides a technical scheme of taking the biogas slurry as a basic solution and scientifically compounding functional additives. Each liter of basic liquid of the preparation comprises 1.0-3.0 g of potassium humate, 0.5-2.0 ml of sugar alcohol calcium and 0.5-2.0 g of carboxymethyl chitosan, and at least one of 0.3-1.0 g of bacillus subtilis powder with the effective viable count being greater than or equal to 10 billion / g or 0.1-0.3 ml of a brassinolide aqueous solution with the concentration being 0.01% is selectively added. The preparation is mainly used for improving the fruit quality of grapes, and high-yield and high-quality cultivation of the grapes is realized through multiple effects of synergistically providing nutrients, promoting absorption, enhancing stress resistance and the like.