588 results about "Brassica" patented technology

The invention provides an anti-premature senility management method for closely-planted double-layer truncated cone macadamia nut trees, and belongs to the technical field of fruit tree cultivation. The premature senility prevention management method comprises layered nutrition supply and flower and fruit protection management. The layered nutrition supply comprises the following steps: deeply applying a calcium magnesium phosphate fertilizer to the lower layer of soil greater than 60cm, and covering a root controller; organic fertilizers and biological agents are applied to 30-60 cm soil of the middle layer; a macroelement, humic acid and seaweed extract water-soluble fertilizer is applied to the 0-30 cm soil of the upper layer; the flower and fruit protection management comprises the steps that ABA and paclobutrazol are sprayed in the flower bud differentiation period; brassinolide and fluid boron are sprayed in the full-bloom stage; spraying forchlorfenuron and amino acid in a young fruit stage; nAA and monopotassium phosphate are sprayed in the fruit swelling period. Layered nutrition supply and flower and fruit protection management are carried out on the densely-planted double-layer truncated cone macadamia nut trees, premature senility of the densely-planted macadamia nut trees is prevented, branch development of the lower layer is promoted, the tree vigor is balanced, and the high-yield level is maintained.

The invention discloses a breeding method of large-seed and high-oil type brassica napus L. The disclosed method comprises the following steps: knocking out a BnaWIP2 gene in brassica napus L., so that the BnaWIP2 gene is mutated to obtain the large-seed and high-oil type brassica napus L.. According to the method, six copies of the BnaWIP2 gene are knocked out at the same time, the large-seed and high-oil brassica napus CRISPR / Cas9 gene editing material is obtained, and the gene editing material provides precious gene resources and germplasm resources for breeding of high-oil and high-yield brassica napus varieties.

The invention belongs to the technical field of crop molecular markers, and particularly discloses a molecular marker related to the waterlogging tolerance of brassica napus and application of the molecular marker. According to the invention, a 1.2 Kb transposon insertion and a 2.6 Kb transposon insertion are identified by utilizing GWAS (Good White Attenual Sequence) and are positioned in a promoter region of the Bna.C08. GUN4 gene, and the rape containing the two transposon insertion has high waterlogging resistance. The molecular marker is used for auxiliary selection of oilseed rape with strong waterlogging resistance, whether the oilseed rape germplasm is waterlogging-resistant oilseed rape germplasm can be predicted only by detecting the characteristic band of the molecular marker, the identification method is simple and feasible, the selection efficiency is high, and the molecular marker has huge application potential in the field of oilseed rape waterlogging resistance improvement breeding.

The invention relates to the technical field of agricultural biology, in particular to application of a BnaC04.bZIP16 gene to improvement of salt tolerance of plants. The sequence of the BnaC04.bZIP16 gene provided by the invention is as shown in SEQ ID NO.1, and the coded protein sequence of the BnaC04.bZIP16 gene is as shown in SEQ ID NO.2. The invention discovers that the BnaC04.bZIP16 gene can be highly expressed after wild brassica napus is irrigated with saline water, and meanwhile, the transgenic brassica napus with the over-expressed gene can better grow in a salt stress environment, so that the BnaC04.bZIP16 gene can be used for preparing a salt-tolerant brassica napus. The rape with the BnaC04.bZIP16 gene knocked out is more difficult to grow in a salt stress environment, so that the effect of the BnaC04.bZIP16 gene as a forward regulatory factor in enhancing the salt tolerance of the rape is verified, and a key molecular tool is provided for developing a new variety of brassica napus with high salt tolerance; the gene has wide application potential and commercial value in the field of agricultural planting, especially in improving the salt tolerance of crops and cultivating transgenic crops with high salt tolerance.

The invention belongs to the technical field of biology, and discloses a method and application for regulating and controlling the fermentation process of schizochytrium limacinum through plant hormones to improve the oil titer, and the method is characterized in that in the fermentation process of schizochytrium limacinum, one or more plant hormones are added into a culture medium to promote accumulation of oil. Plant hormones are added into a fermentation culture medium of schizochytrium limacinum, the plant hormones comprise gibberellin (GB), 6-benayl aminopurine (6-BAP), forchlorfenuron (CPPU) and brassinolide (BR), and the plant hormones promote grease accumulation by adjusting activity of enzymes related to fatty acid synthesis, so that the plant hormones can adjust metabolic pathways of the schizochytrium limacinum and promote grease accumulation. The synthesis efficiency and the yield of the grease are obviously improved.

The invention discloses a water and fertilizer management method for transplanted high-yield indica type two-line hybrid rice, and belongs to the technical field of rice cultivation. Comprising the following steps: (1) applying citric acid chelated calcium magnesium phosphate fertilizer to soil before transplanting; (2) applying coated controlled-release urea and a silicon-zinc fertilizer on the third day after transplanting; (3) when the tiller number reaches 2650,000-2750,000 plants / hm < 2 >, starting intermittent light field drying for three times, and deeply applying potassium magnesium sulphate before field drying; (4) laterally and deeply applying urea and a boron-humic acid chelate when tips of two inverted leaves are exposed; (5) spraying an aqueous solution in a full heading stage, wherein the aqueous solution comprises monopotassium phosphate, chelated zinc and brassinolide. Through staged precise fertilization, special chelating / controlled release fertilizer and water and fertilizer-field drying cooperation, the method has the advantages that the fertilizer utilization rate is increased, the nutritional requirements of rice in each growth period are precisely matched, and water and fertilizer cooperative management is optimized.

The invention discloses development and application of a molecular marker of a rape stain tolerance related gene BnaERF3, and belongs to the technical field of molecular biology. According to the invention, the BnaA06. ERF3 gene is screened on the basis of whole genome associated molecules, and is used as a key gene in QTL (qWL.A06) to participate in regulation and control of the waterlogging tolerance of the rape. A molecular marker related to the waterlogging tolerance of the brassica napus is developed aiming at the BnaA06. ERF3 gene, and tests show that the waterlogging tolerance of the brassica napus can be detected by utilizing the molecular marker, so that varieties containing waterlogging tolerance genes are subjected to auxiliary selection and breeding, and the waterlogging tolerance breeding process of the brassica napus is accelerated.

The invention belongs to the field of crop science and agricultural molecular biology, and particularly relates to application of a BAK gene to enhancement of flood tolerance of rape, and the BAK gene is a BnaA04.BAK7 gene; the login number of the BnaA04. BAK7 gene in a gene pool is BnaA04g015000WE, and the login number of the BnaA04. BAK7 gene in the gene pool is By destroying the expression of the BAK gene, it is found that after the expression of the BAK gene is destroyed, the flooding tolerance of the brassica napus under flooding stress is weakened, and the invention discloses for the first time that hypocotyl growth of the brassica napus under flooding stress is slow and chlorophyll content is reduced due to function deletion of the BAK gene. New gene resources and theoretical guidance are provided for research and application of the flood tolerance of the rape.

Provided in the present invention are a Brassica napus drought-tolerant gene BnaC04ARL and the use thereof. The gene is expressed in a plant to improve drought tolerance of the plant. The gene comprises a nucleotide sequence selected from the group consisting of: a nucleotide sequence shown as SEQ ID NO: 1; and / or a nucleotide sequence encoding an amino acid sequence shown as SEQ ID NO: 2. The overexpression of the gene can improve the viability of Brassica napus under drought conditions, enhance root system development, and improve the thousand-grain weight, the grain yield per plant, the number of siliques per plant, dry weight per plant, silique length, etc.

According to the application of the BnaGSTU11 gene in regulating and controlling the sclerotinia rot resistance of brassica napus, the amino acid sequence is as shown in SEQ ID NO: 2. According to the invention, a gene BnaGSTU11 for coding glutathione S-transferase is separated and cloned from brassica napus, and protease coded by the gene can enhance the sclerotinia rot resistance of the brassica napus by removing active oxygen and free radicals. The discovery of the sclerotiniose-resistant gene BnaGSTU11 provides a new genetic resource for cultivating a new sclerotiniose-resistant rape germplasm, and also provides a new thought for mining sclerotiniose-resistant genes of other crops.

The invention discloses a special growth regulator for pepper based on compounding of propionyl brassinolide and amino-oligosaccharin and application of the special growth regulator, and belongs to the technical field of agricultural biology. According to the regulating agent, 0.0008 to 0.0012 percent of propionyl brassinolide and 2.95 to 3.05 percent of amino-oligosaccharin are accurately matched with each other. The formula breaks through the contradiction between the root promotion requirement of high-dose amino-oligosaccharin and the safety threshold of low-dose brassinolide, and overcomes the phytotoxicity risk of the traditional dosage form. The regulator prepared by the invention can enhance the growth vigor of the pepper, improve the product and quality of the pepper, and also can effectively prevent viral diseases of the pepper.

The invention discloses a traditional Chinese medicine fermentation composition with a qi and blood nourishing function and a preparation method thereof, and belongs to the technical field of health care products. The traditional Chinese medicine fermented composition is prepared from the following raw materials in parts by weight: 15-20 parts of codonopsis pilosula, 12-18 parts of angelica sinensis, 5-10 parts of longan aril, 3-7 parts of purslane, 2-6 parts of brassica juncea, 4-9 parts of amomum tsao-ko and 5-11 parts of asparagus cochinchinensis. The traditional Chinese medicine fermentation composition is prepared through synergistic compatibility of various traditional Chinese medicines, an efficient extraction process and a multi-strain synergistic fermentation process, the qi and blood tonifying effect of the traditional Chinese medicine fermentation composition can be remarkably improved, and an innovative solution is provided for industrial production of qi and blood tonifying health care products.

The invention belongs to the technical field of plant molecular markers, and particularly discloses an Indel molecular marker related to the salt tolerance of brassica napus and application of the Indel molecular marker. The marker is located at the position of an A01 chromosome scafoldA0133077947 of a brassica napus reference genome ZS11.v10, and the genotype of a polymorphic site is CTAA or C. The invention further discloses a method for identifying the marker of the brassica napus. Large-scale group phenotypic association analysis proves that the molecular marker is remarkably associated with the salt tolerance of the brassica napus in the germination period, and when the polymorphic site genotype is CTAA, the salt tolerance in the germination period is high; when the genotype of the polymorphic site is C, low salt tolerance in the germination period is shown. The invention finds that the polymorphic site is obviously associated with the salt tolerance of the brassica napus for the first time, provides a powerful molecular tool for solving the problems of difficulty in phenotype identification, long period and low efficiency in salt tolerance breeding of the brassica napus, can accurately screen salt-tolerant materials in the early stage, and greatly accelerates the breeding process of new varieties of the salt-tolerant brassica napus.

The invention provides an application of a Brassica napus gene BnSKIP31 in prevention and control of sclerotiniose, which is an application in obtaining a disease-resistant enhanced rape material by creating a gene to edit rape. According to the invention, CRISPR gene knockout rape of BnSKIP31 is constructed, the negative regulation function of the gene on sclerotiniose resistance is disclosed for the first time, and application of the BnSKIP31 gene to acquisition of sclerotiniose rape materials with increased sclerotiniose resistance by creating CRISPR gene knockout rape is provided. The BnSKIP31 gene provided by the invention is a new gene resource suitable for creating and breeding new sclerotiniose-resistant rape materials and new varieties, and the created rape germplasm has lasting disease resistance.

The invention provides a long-acting brassinolide compound and application thereof. The structure of the long-acting brassinolide compound is shown as a formula I or a formula II. The long-acting brassinolide compound provided by the invention is used in tea tree planting, can effectively increase the yield of tea leaves and improve the quality of the tea leaves, and has more excellent technical effects compared with other reagents.

The invention discloses a molecular marker related to the oil content of brassica napus and application of the molecular marker, and belongs to the technical field of molecular marker assisted breeding. A transcription factor BnaA09.MYB52 for coding an MYB structural domain is proved to be capable of influencing the oil content of cabbage type rape seeds, molecular markers corresponding to different haplotypes of the gene and primers of the molecular markers are designed by utilizing re-sequencing data, and the DNA molecular markers can be used for distinguishing the functional haplotypes of the gene of a cabbage type rape strain to be detected; the gene molecular marker can be used for detecting and predicting the oil content of the brassica napus, can also be used for molecular marker-assisted selective breeding of the high-oil-content brassica napus, can effectively accelerate the process of high-oil-content breeding of the brassica napus, is suitable for large-scale popularization and application, and has a wide application prospect.

The invention provides an amylose content regulator and application thereof in improving the amylose content of wheat, and relates to the technical field of plant variety improvement. The amylose content regulator is mainly prepared from 24-brassinolide with the concentration of 0.2 to 25 [mu] g / L, abscisic acid with the concentration of 0.05 to 6.25 mg / L, zinc sulfate with the concentration of 0.1 to 1 wt% and boric acid with the concentration of 0.01 to 0.1 wt%. The invention also provides an application of the amylose content regulator in increasing the amylose content of wheat. The method specifically comprises the step of spraying the amylose content regulator on the surfaces of wheat plants in a wheat filling period so as to increase the amylose content of the wheat. The growth period of wheat is regulated and controlled through the amylose content regulator, so that the amylose content of the mature wheat seeds is increased, the method is easy and convenient to operate, and the amylose content of the treated mature seeds is increased by about 9%-16% compared with a contrast.

The invention relates to the technical field of biological genetic engineering, in particular to application of a brassica napus BnaA01G0331000ZS gene in drought stress resistance. The BnaA01G0331000ZS gene is cloned by using cDNA of double 11 leaves as a template through a sequence published in a brassica napus genome database, an over-expression vector is constructed and genetically transformed into rape variety high oil for transgenic function verification, the drought resistance of the obtained over-expression transgenic line is obviously enhanced compared with that of a wild type, and the BnaA01G0331000ZS gene has the advantages that the BnaA01G0331000ZS gene is obtained; therefore, the gene can positively regulate the drought stress resistance of the rape. Therefore, the invention provides a new gene resource for adversity stress resistance of rape.

The invention relates to the technical field of crop planting, and provides a method for promoting germination of brassica napus seeds under low-temperature stress by using allantoin. The method comprises the following steps: firstly, disinfecting cabbage type rape seeds, and then soaking the cabbage type rape seeds in an allantoin solution with a specific concentration under room-temperature and weak-light conditions; the treated seeds do not need to be washed and are directly placed in a culture dish paved with double-layer filter paper, a proper amount of distilled water is added, and then the seeds are cultured in an incubator simulating a low-temperature environment. Experimental results show that the germination rate, the germination index and the vitality index of the brassica napus seeds are remarkably improved through allantoin treatment, and the average germination time is shortened. Mechanism research finds that allantoin improves the low-temperature resistance of the brassica napus by various ways of increasing the content of free water of seedlings, increasing the content of soluble sugar and proline, enhancing the activity of antioxidant enzyme, reducing active oxygen and malonaldehyde accumulation and the like. The method disclosed by the invention is low in cost and environment-friendly, and provides powerful guarantee for field production such as late direct seeding of the brassica napus.

The invention provides a method for identifying the low temperature resistance of brassica napus in the flowering period through the silique length, and belongs to the technical field of rape low temperature resistance germplasm resource identification, the method comprises the following steps: 1) directly sowing brassica napus in a field in mid-to-late October in the current year; (2) in March of the second year, when the daily average temperature is lower than 10 DEG C for 3 days continuously, performing low-temperature treatment marking on the brassica napus; (3) when the daily average temperature is higher than 16 DEG C for 3 days, determining the extending siliques as a normal-temperature treatment group of the brassica napus; (4) the silique lengths of the low-temperature treatment group and the normal-temperature treatment group are measured respectively, the silique low-temperature-resistant index is calculated, and the silique low-temperature-resistant index is equal to the average silique length of the normal-temperature treatment group / the average silique length of the low-temperature treatment group. And determining the flowering phase low temperature resistance of the to-be-detected brassica napus variety according to the silique low temperature resistance index. The method disclosed by the invention is simple to operate, and can be used for quickly and effectively identifying the germplasm with low temperature resistance in the flowering phase in a field environment.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a brassica napus BnaCP15A gene coded protein to improvement of plant salt tolerance. The technical problem to be solved by the invention is to provide a new choice for improving the salt tolerance of plants. The technical scheme of the invention is the application of the protein coded by the brassica napus BnaCP15A gene in improving the salt tolerance of the plant, wherein the amino acid sequence of the protein is shown as SEQ ID NO.2. The invention further discloses the application of the protein coded by the brassica napus BnaCP15A gene. The salt tolerance of the plant is improved by increasing the expression of the brassica napus BnaCP15A gene in the plant, and the nucleotide sequence of the BnaCP15A gene is as shown in SEQ ID NO. 1. The main purpose of the invention is to provide the application of the protein coded by the brassica napus BnaCP15A gene in improving the salt tolerance of the plant, a new choice is provided for improving the salt tolerance of the brassica napus, and the brassica napus BnaCP15A gene has important application value.

The invention belongs to the technical field of fertilizers, and particularly relates to a compound plant growth regulator composition. The composition is prepared from gibberellic acid, indoleacetic acid, brassinolide and thidiazuron. According to the technical scheme, on the basis of the ternary compound plant regulator, thidiazuron is further added and cooperates with 14-hydroxyl brassinosteroid in the ternary compound, the activity of the plant regulator is further improved, the composition can well adapt to various fruit trees, growth of capillary roots of crops is effectively promoted, and the growth rate of the capillary roots of the crops is increased. Rapid rooting, flower strengthening and fruit swelling, and comprehensive regulation of plants. According to the technical scheme, thidiazuron is further added to cooperate with 14-hydroxyl brassinosterol in the ternary compound, and the effect of the composition is more remarkable than that of a composition using a single compound or not containing thidiazuron. The composition can be well adapted to various fruit trees, effectively promotes the growth of capillary roots of crops, rapidly roots, strengthens flowers and expands fruits, and realizes comprehensive regulation. The yield increasing rate of various crops is obviously increased, the central sugar increasing rate can be kept to be 16% or above, and the rotten fruit rate of citrus can be controlled to be 20% or below.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of a BnaWLT8 gene to improvement of waterflooding stress resistance of rape. The nucleotide sequence of the BnaWLT8 gene is as shown in SEQ ID NO. 1. The oilseed rape waterlogging resistance related gene BnaWLT8 provided by the invention provides an excellent gene resource and a new effective way for improving the waterlogging stress resistance of oilseed rape and cultivating a new variety of waterlogging-resistant brassica napus. Through transgenic genetic breeding, the expression level of the BnaWLT8 gene is enhanced, the waterlogging resistance and flooding resistance of the rape in the germination stage and the seedling stage can be remarkably enhanced, and the BnaWLT8 gene has wide application prospects and good social benefits in the aspects of stress resistance improvement of the rape and the like.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker for auxiliary selection of the leaf number of brassica napus, a primer group, a detection method and application, and relates to the technical field of biology and rape breeding. The SNP marker is located at the 9283404 bp position of a brassica napus A02 chromosome, a basic group of the site is a T allele or a C allele, and the T allele is associated with a leafy character. The SNP marker provided by the invention can solve the technical problem that the breeding efficiency of the high-photosynthetic-efficiency ideal-plant-type rape variety is restricted due to the fact that an effective molecular marker closely linked with the leaf number character of the brassica napus is lacked in the prior art and the character cannot be quickly and accurately screened in the early breeding stage.

The invention discloses application of N-acetyl-L-cysteine in improving drought tolerance of plants, and belongs to the technical field of plant cultivation. The invention provides the application of N-acetyl-L-cysteine (N-acetyl-L-cysteine, NAC) in the improvement of the drought tolerance of the brassica napus for the first time. Experiments prove that compared with a control group, the fresh weight, the dry weight, the root length and the chlorophyll content of rape seedlings subjected to foliage spraying of 150 mu M of N-acetyl-L-cysteine under the drought stress condition are improved, the water loss rate of leaves is reduced, and the drought stress tolerance of the rape is enhanced. Therefore, the drought stress tolerance of the rape treated by the N-acetyl-L-cysteine is obviously improved. Furthermore, an optimal application concentration of 150 mu M is obtained through experimental exploration, so that the N-acetyl-L-cysteine can be optimally utilized in improving the drought stress tolerance of the oilseed rape.

A method for improving the germination rate and seedling growth vigor of scutellaria baicalensis seeds in a saline-alkali soil environment comprises the following steps: (1) preparing a seed soaking solution, namely preparing a quaternary plant growth regulator combined 6-BA + GA3 + NAA + brassin seed soaking solution; (2) preparing seed dressing mother liquor, namely preparing ternary seed dressing mother liquor of No.6 seed dressing mother liquor, planting elements and pyroligneous liquor; (3) seed soaking: soaking the seeds by using a quaternary seed soaking solution of 6-BA + GA3 + NAA + brassin; (4) airing; (5) seed dressing: carrying out secondary coating by using ternary seed dressing mother liquor of No. 6 seed dressing mother liquor, planting elements and pyroligneous liquor; and (6) sowing: sowing the seeds treated in the step (5) in a matrix containing 0.4% salt stress, and performing conventional culture. Compared with a traditional planting method, the germination rate and seedling growth vigor of the scutellaria baicalensis seeds treated through the method are greatly increased, the germination rate basically reaches 100%, the germination potential is 90%, and the effectiveness and advancement of the method are fully proved.

The invention relates to the technical field of molecular biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker of a Brassica napus Bolima cytoplasm sterile gene, a primer group, application and a method. The SNP (Single Nucleotide Polymorphism) molecular marker is BN9000344. The primer group comprises a primer group BN9000344K01X, a primer group BN9000344K01Y and a primer group BN9000344K01C, and the primer group comprises a primer group BN9000344K01X, a primer The application refers to the application of the SNP molecular marker in detecting whether the brassica napus is of the Brassica napus sterile cytoplasm type or not. The method is used for determining whether the brassica napus is of the Brassica napus sterile cytoplasm type or not. The method can rapidly and accurately detect whether the brassica napus contains the Brassica napus Bolima cytoplasmic male sterility gene or not, and is suitable for large-scale and high-throughput Brassica napus Bolima cytoplasmic male sterility gene identification (such as purity identification of sterile line and maintainer line parents) and screening.

The invention discloses application of beta-glucosidase BnaBG27 in regulation and control of thousand seed weight of rape, and belongs to the technical field of gene engineering. According to the invention, the BGLU27 gene is separated and obtained from brassica napus Westar, and the gene is found to be highly expressed in rape seeds. Experiments find for the first time that the BGLU27 gene positively regulates the thousand seed weight of the rape, and the thousand seed weight of the rape can be increased by utilizing the gene, so that high-yield rape germplasm can be obtained. The method has a very important application prospect in breeding and improvement of high-yield rape varieties.

The invention provides an application of a phosphatidylinositol monophosphate 5-kinase gene BnPIP5K9 of rape in regulating and controlling the oil content of the rape. The genetic basis of the oil content of rape seeds is analyzed on the basis of multiple omics, and it is found that the expression quantity of the phosphatidylinositol monophosphate 5-kinase gene BnPIP5K9 in rape is significantly positively correlated with the oil content; the gene BnPIP5K9 is subjected to gene overexpression and knockout to create a mutant for functional verification by further utilizing a tobacco mosaic virus double-35S promoter and a CRISPR / Cas9 gene editing technology, the result shows that the oil content of rape seeds can be remarkably increased by 2.16-2.77% by overexpression of the gene, in addition, rape germplasm resources with gene BnPIP5K9 function deficiency can be obtained through the CRISPR / Cas9 technology, and the rape seed quality is improved. A new theory and a new gene resource are provided for high-oil breeding and oil improvement of the rape, and the method has extremely high application value and potential.

The invention discloses a brassica napus Bna.A08. SNAT2 protein and application of a coding gene of the brassica napus Bna.A08. SNAT2 protein in salt stress resistance, the Bna.A08. SNAT2 gene is introduced into brassica napus through a genetic engineering technology, and it is found that the tolerance of the brassica napus in a salt stress environment can be remarkably improved through expression of the gene. Bna.A08. SNAT2 protein is used as a key salt-tolerant factor, cells are protected from being damaged by salt stress by adjusting osmotic pressure and ion balance in oilseed rape cells, germination of oilseed rape under salt stress is promoted, cultivation and popularization of salt-tolerant crop varieties are expected to be promoted through further research and application, and the application prospect is wide. Powerful support is provided for solving the problems of saline-alkali soil utilization and grain safety.