707 results about "Medicago sativa" patented technology

The invention discloses an alfalfa SNP (Single Nucleotide Polymorphism) molecular marker combination and application thereof. The SNP molecular marker combination is composed of 61351 SNP molecular markers, the physical positions of the 61351 SNP molecular markers are determined based on sequence alignment of a medicago sativa reference genome ZM-4alfalfa gene, the SNP molecular markers are good in site specificity, high in universality and high in polymorphism, medicago sativa germplasms of different sources can be effectively distinguished, and the application prospect is wide. The alfalfa whole-genome liquid-phase gene chip prepared from the SNP molecular marker can be used for alfalfa germplasm resource genetic diversity evaluation, genetic map construction, functional gene positioning, whole-genome association analysis and the like, and provides important tools and technical supports for alfalfa molecular design breeding and variety authenticity identification. The method has important significance on high-quality development of alfalfa seed industry in China.

The present invention relates to the use of MtNAC33 gene and a protein encoded thereby in the cultivation of high-biomass and drought-tolerant alfalfa, belonging to the technical field of plant genetic engineering. The plant protein encoded by MtNAC33 gene has the amino acid sequence set forth in SEQ ID NO: 2, and MtNAC33 gene has the nucleotide sequence set forth in SEQ ID NO: 1. In the present invention, the drought tolerance of Arabidopsis thaliana and alfalfa can be significantly improved by regulating the expression level of MtNAC33 in alfalfa. The phenotypes of delayed flowering, increased leaf-to-stem ratio and more biomass in alfalfa were also developed, which has an important effect for genetic improvement of stress resistance and biomass of forage grass and other crops.

The invention discloses application of a gene FTO in improvement of forage grass quality, yield and stress resistance. According to the invention, the FTO gene is subjected to codon optimization according to the metatropism of alfalfa and is overexpressed in alfalfa to obtain a transgenic alfalfa plant overexpressed with the FTO gene, and compared with wild alfalfa, the transgenic alfalfa plant overexpressed with the FTO gene has the advantages that the growth performance is enhanced; the crude protein content, the soluble carbohydrate content and the in-vitro dry matter digestibility are obviously higher than those of the wild type; the crude fiber content, the acid detergent fiber content, the neutral detergent fiber content and the lignin content of the transgenic alfalfa plant are obviously lower than those of a wild type; in addition, the drought resistance and salt stress resistance of the transgenic alfalfa plant are also obviously enhanced, which indicates that the FTO gene has the functions of improving forage grass quality, resisting stress or positively regulating plant growth. The method has application prospects in the aspects of improving forage grass quality and stress resistance or promoting plant growth and the like.

The invention provides an easy-to-swallow nutrition-enriched plant-based protein food and a preparation method thereof, the plant-based protein food comprises a mixed protein solution and edible oil, and the mixed protein solution comprises walnut protein, soybean protein isolate, alfalfa protein and egg white protein. The preparation method of the plant-based protein food comprises the following steps: preparing a high-protein emulsion from a mixed protein solution and edible oil and fat, preparing emulsion gel from the high-protein emulsion, and performing 3D printing by adopting the emulsion gel. According to the easy-to-swallow type nutrition-enriched plant-based protein food disclosed by the invention, four proteins are compounded, the nutritional value of the food is improved through the synergistic effect, the protein content is increased, amino acid compounding is realized, and meanwhile, the rheological property and extrusion property of 3D printing after protein compounding are improved, so that the printed food can be suitable for 3D printing and also can be used for 3D printing. The food can also be applied to old people with dysphagia, and meets the requirements of daily nutrition intake and easy swallowing.

The invention relates to the technical field of plant genetic engineering, in particular to an application of an alfalfa MsPL19 protein and a gene thereof in regulating and controlling the plant height, fresh weight or biological yield of alfalfa. The invention provides an MsPL19 protein, a gene and application thereof, which can obviously improve the plant height, the biological yield and the fresh weight of medicago sativa and further improve the quality of the medicago sativa. Meanwhile, it is found that the MsPL19 protein and gene can respond to low temperature, drought, salt, hormone and other pathways, the protein interacting with MsPL19 is obtained through immunoprecipitation-mass spectrometry analysis, corresponding molecular modules can be enriched, the molecular mechanism of alfalfa MsPL19 responding to the low-temperature environment is analyzed, new cold-resistant varieties can be bred for alpine regions, and the application prospect is broad. And a new breeding direction is provided for increasing the yield of alfalfa.

The invention discloses a saline-alkali soil improver and a use method thereof, and belongs to the technical field of soil improvement. The modifier comprises the following components in parts by mass: 60-80 parts of an organic carrier, 10-20 parts of an inorganic calcium material, 5-9 parts of a compound microbial agent, 10-20 parts of a sustained-release material, 5-10 parts of an acidity regulator and 15-23 parts of a plant growth promoter. The preparation method comprises the following steps: crushing the raw materials, embedding the sustained-release microspheres, loading the microbial inoculum, and granulating and drying. Through cooperation of multiple mechanisms such as calcium and sodium replacement, pH reduction by organic acid and aggregate formation promotion by microorganisms, differential application strategies are designed for light, medium and severe saline-alkali soil, salt-tolerant crop planting is matched, the yield of wheat, alfalfa and the like is increased by 18%-30%, the soil organic matter is increased to 2.1%, and the microbial diversity index is increased by 133%. The method has the advantages of long-acting salt control, ecological restoration and cost, and is suitable for treatment of coastal and inland saline-alkali soil.

The invention relates to the field of alfalfa planting, in particular to a synthetic flora for breaking dormancy of alfalfa seeds and promoting growth of seedlings and application of the synthetic flora. The invention provides a strain of sinorhizobium meliloti NFB5, the preservation number is CGMCC NO.33618, and the sinorhizobium meliloti NFB5 can break dormancy of alfalfa seeds and promote growth of alfalfa seedlings and root systems. According to the method, sinorhizobium meliloti NFB5 is compounded with one or two of screened salt-tolerant brevibacillus brevis EB3 and bacillus PS06 to obtain a synthetic flora, and alfalfa seeds are soaked in a bacterial solution containing the synthetic flora, so that the number of hard seeds can be remarkably reduced, the germination rate and germination potential of the seeds can be remarkably improved, and the germination rate of the seeds can be remarkably increased. Therefore, the synthetic flora can well break the dormancy of the alfalfa seeds, does not cause loss to the seeds, and does not reduce the quality of the seeds. In addition, the synthetic flora also has a significant growth promoting effect, and can significantly improve the plant height, root length, stem weight and root weight of seedlings.

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention discloses an irrigation device and method for alfalfa cultivation in saline-alkali soil, and relates to the technical field of alfalfa cultivation, the irrigation device comprises a water storage tank and an anti-blocking irrigation mechanism, the anti-blocking irrigation mechanism comprises an irrigation cylinder, an irrigation pipe and a spiral head, the irrigation cylinder is fixedly installed in the middle of the bottom end of the water storage tank through a bolt, and the spiral head is connected with the irrigation pipe; the irrigation pipe is fixedly installed in the middle of the bottom end of the irrigation cylinder through bolts, and the spiral head is movably arranged at the bottom end in the irrigation cylinder. Water in the water storage tank and the irrigation pipe is sucked and then discharged through the suction cylinder, the irrigation water consumption can be accurately controlled conveniently, a spiral head is driven to move downwards and rotate while a piston reciprocates, so that when the device is inserted into soil, the bottom end, blocked by the soil, of the irrigation pipe can be rotationally conducted by the spiral head, and the irrigation efficiency is improved. Soil below the bottom end of the irrigation pipe is drilled out of a water seepage space through the spiral head which ascends and descends in a reciprocating mode, the irrigation effect is further improved, and the irrigation efficiency when alfalfa is cultivated in the saline-alkali soil is improved.

The invention relates to a special organic compound fertilizer for alfalfa as well as a preparation method and application thereof, and relates to the technical field of organic fertilizers, the special organic compound fertilizer for alfalfa comprises the following components in parts by weight: 50-60 parts of a basic organic fertilizer, 20-30 parts of a sodium humate-seaweed compound, 15-20 parts of modified phosphorus-potassium ore powder, 0.5-1 part of a phosphate-solubilizing bacterium agent and 0.5-1 part of an alfalfa rhizobium agent. On the basis of the existing basic organic fertilizer, the sodium humate-seaweed compound and the modified phosphorus-potassium ore powder loaded with the appropriate fungicide are mainly added, so that the yield of the alfalfa is remarkably increased, and the special organic compound fertilizer can be used as the special organic compound fertilizer for the alfalfa and is wide in application.

The invention relates to the technical field of plant genetic engineering, and particularly discloses an alfalfa salt-alkali-resistant and low-temperature composite stress-resistant gene MsNCED5 and application of encoded protein thereof in improving salt-alkali-resistant and low-temperature composite stress-resistant capability of plants. The nucleotide sequence of the gene disclosed by the invention is as shown in SEQ ID NO.1, the full length is 1833 bp, and 610 amino acids are encoded. According to the invention, an MsNCED5 overexpression vector is constructed and medicago sativa is converted, so that an MsNCED5-OE transgenic line is successfully created, and the MsNCED5 gene expression quantity of the MsNCED5-OE transgenic line is obviously increased compared with that of a wild type. Under the salt-alkali and low-temperature composite stress, the transgenic plant has stronger stress tolerance compared with the wild plant, which indicates that the overexpression of the MsNCED5 gene improves the resistance of the plant to the salt-alkali and low-temperature composite stress. The MsNCED5 gene disclosed by the invention provides an important theoretical basis for researching an alfalfa stress resistance molecular mechanism and breeding.

The present invention belongs to the technical field of saline-alkali land improvement, and specifically relates to a method for improving saline-alkali land based on an efficient cultivation mode of alfalfa, oats and okra, comprising the following steps: S1. Plant alfalfa on the saline-alkali land, and let the alfalfa overwinter after being mowed; S2. Interplant oats and alfalfa in rows, and harvest the oats after they mature; S3. After harvesting the oats, plant okra in the planting area of the oats. The okra and alfalfa are interplanted in rows. After the okra matures, pick the okra, then cut the above-ground part of the okra and spread it flat between the planting rows; S4. During the planting period of oats and okra, mow the alfalfa multiple times, and let the alfalfa overwinter after the last mowing. By means of the efficient cultivation mode of interplanting alfalfa and oats, interplanting alfalfa and okra, and rotating oats and okra, the present invention improves the yield and quality of alfalfa and enhances the improvement effect of alfalfa on saline-alkali land.

The invention discloses a sporosarcina sp. Strain RH34 and an application of the sporosarcina sp. Strain RH34 and the application of the sporosarcina sp. Strain RH34. The sporosarcina strain RH34 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33199. The sporosarcina strain RH34 can be used for preparing the sporosarcina strain RH34. The alfalfa rhizosphere microorganism sporosarcina strain RH34 is separated from alfalfa roots in salinized soil, and has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing biological membranes, producing siderophores, producing IAA and producing ACC deaminase. Seed soaking and germination under salt stress can obviously improve the germination index of alfalfa seeds. The growth condition of alfalfa seedlings can be improved under salt stress.

The invention discloses a fertilizer special for lotus roots and a preparation method thereof, and belongs to the technical field of planting. The special fertilizer for the lotus roots is prepared from the following raw materials in parts by mass: 170 to 190 parts of urea, 25 to 35 parts of P2O5, 105 to 120 parts of K2O, 10 to 15 parts of trace elements, 10 to 15 parts of alfalfa, 8 to 12 parts of a mixed fermentation product of Chinese ilex and willow branches, 10 to 15 parts of plant waste activated carbon and 20 to 25 parts of a slow release agent. The special fertilizer for the lotus roots can prolong the fertilizer efficiency and promote the growth of the lotus roots; direct contact between the fertilizer and the outside is reduced, the release rate of nutrients in the special fertilizer for lotus roots is controlled, the physical and chemical properties of the fertilizer and the soil structure are improved, and the yield of lotus roots is increased; the special fertilizer for the lotus roots, prepared by the invention, can be applied with chemical fertilizer once in the whole growth period of the lotus roots without topdressing, so that the harm of excessive fertilizer to the environment and crops is reduced, and the fertilization cost is reduced.

The invention relates to paenibacillus polymyxa capable of preventing diseases, resisting stress and promoting growth and application of the paenibacillus polymyxa, and belongs to the technical field of microorganisms, the paenibacillus polymyxa SS44 has the characteristics of degrading protein, degrading fat, degrading cellulose, dissolving potassium, fixing nitrogen, dissolving organic phosphorus, dissolving inorganic phosphorus and producing exopolysaccharide. The strain is capable of antagonizing pathogenic bacteria fusarium oxysporum and fusarium fusiforme which can cause potato fusarium wilt, sweet potato root rot, soybean root rot, alfalfa root rot and the like, can improve sandy soil, can generate a good growth promoting effect on wheat, soybean and corn in saline-alkali soil and sandy barren soil environments, and is very wide in application prospect.

The present invention provides a biological seed treatment agent, which comprises: a microbial agent, which is selected from at least one of Bacillus polymyxa and Bacillus amyloliquefaciens; and an iron-containing compound. The biological seed treatment agent of the present invention has the advantages of increasing seed germination rate, improving seedling quality, improving stress resistance, preventing and controlling pests and diseases, increasing crop yield, reducing fertilizer usage, etc., and is particularly suitable for the prevention and control of wheat sheath blight, soybean root rot and alfalfa damping-off, and has good application prospects.

The invention belongs to the technical field of plant genetic engineering, and relates to medicago sativa polygalacturonase MsPG2, a coding gene thereof and application of the medicago sativa polygalacturonase MsPG2 in plant salt stress resistance, and an amino acid sequence of the polygalacturonase MsPG2 is shown as SEQ ID NO.1 or a derivative sequence or a homologous sequence of the amino acid sequence shown as SEQ ID NO.1. The invention further discloses a preparation method of the medicago sativa polygalacturonase MsPG2. The invention also provides a coding gene of the polygalacturonase MsPG2, wherein the nucleotide sequence of the coding gene is shown as SEQ ID NO. 2; the coding gene can be expressed by salt induction. According to the invention, the cultivation of the MsPG2 transgenic plant is realized by utilizing a genetic engineering technology, the salt tolerance of the plant is obviously improved, an important theoretical basis is provided for the cultivation and breeding work of a new salt-tolerant variety of medicago sativa, and the MsPG2 transgenic plant has a great application value.

The invention relates to the technical field of plant genetic engineering, in particular to a medicago sativa MsAST gene and application thereof in regulation and control of plant salt tolerance. The nucleotide sequence of the medicago sativa MsAST gene is as shown in SEQ ID NO. 1. According to the invention, the salt tolerance-related MsASTT gene is cloned from alfalfa, a recombinant vector of the MsASTT gene is constructed, the function of improving the salt tolerance of plants by the MsASTT gene is verified in arabidopsis thaliana, and the MsASTT gene can be used for improving the salt tolerance of alfalfa and creating an alfalfa germplasm material with strong salt tolerance.

The invention relates to the technical field of plant growth regulation, in particular to a pesticide preparation and method for promoting yield increase and branching of medicago sativa L. The pesticide preparation is prepared from, by weight, 1-4 parts of benayl aminopurine, 0.1 part of compound sodium nitrophenolate, 1-4 parts of brassinolide, 1-3 parts of triacontanol and 1-4 parts of uniconazole. When the medicago sativa grows to a seedling stage after being sown, the pesticide preparation is applied after the medicago sativa is cut on the ground, branching of the medicago sativa can be effectively promoted, the yield of the medicago sativa is increased, and the quality of the medicago sativa cannot be reduced.

The invention relates to the technical field of plant breeding, and particularly discloses a metabonomics-based alfalfa stress resistance gene analysis function and a breeding method. The core of the method is that screening is carried out by combining a tissue culture technology with metabolic intervention; an alfalfa explant is inoculated into an induction culture medium containing a specific metabolic pathway regulator for metabolic intervention culture; then transferring the obtained callus to a verification culture medium containing the metabolism regulator and an abiotic adversity stress factor at the same time, and carrying out metabolism mark verification and screening; and finally regenerating the screened cell line into a complete plant. According to the method, metabolic pressure is actively applied to the cellular level, and dual verification screening is implemented, so that early-stage, efficient and accurate breeding of the stress-resistant alfalfa material is realized, and the technical problems of long period, low efficiency and insufficient utilization of metabolic information in traditional breeding are solved.

Provided are a Medicago sativa SNP molecular marker combination and a use thereof. The SNP molecular marker combination consists of 61351 SNP molecular markers. The physical positions of the 61351 SNP molecular markers are determined by sequence alignment on the basis of the Medicago sativa reference genome ZM-4 alfalfa genome. The SNP molecular markers have good site specificity, strong versatility, and high polymorphism and can effectively discriminate Medicago sativa germplasms from different sources. A Medicago sativa genome-wide liquid-phase gene chip is prepared from the SNP molecular markers, and can be used for genetic diversity assessment of Medicago sativa germplasm resources, genetic map construction and functional gene mapping, genome-wide association study, etc., providing an important tool and technical support for Medicago sativa molecular design breeding and variety authenticity identification, and achieving important significance for high-quality development of the alfalfa seed industry in China.

The invention provides an MsLEA3 gene and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, the alfalfa MsLEA3 gene is subjected to overexpression or gene editing is carried out on the MsLEA3 gene in alfalfa through a CRISPR / Cas9 system, so that an overexpression transgenic line of the alfalfa MsLEA3 gene or a gene editing transgenic line aiming at the alfalfa MsLEA3 gene is obtained. The salt tolerance of an overexpressed or gene-edited medicago sativa transgenic line of the MsLEA3 gene is remarkably improved, the MsLEA3 gene can be used for cultivating medicago sativa varieties with high salt resistance, a new theoretical basis and candidate genes are provided for cultivating new varieties of high-resistance leguminous forage, and the application of medicago sativa in production is greatly promoted.

The invention discloses a method for identifying and counting alfalfa seed pods with different maturity degrees based on RHE-DETR, and belongs to the technical field of target detection. The method comprises the following steps: acquiring alfalfa seed pod images under different illumination conditions and shielding conditions in a field; labeling software is used for labeling the collected images, and an alfalfa seed pod data set is constructed and divided; the model is improved, and a lightweight target detection model RHE-DETR for a small target is obtained; inputting the constructed alfalfa seed pod data set into an RHE-DETR target detection model for training to obtain a weight file of the model after the alfalfa seed pod data set is trained; and deploying a weight file trained by the RHE-DETR target detection model to an edge computing platform to detect a field alfalfa seed pod image to obtain a real-time tracing frame, confidence and counting result of the alfalfa seed pod. According to the method, the conditions of error detection and missing detection in a complex field environment are reduced, the accuracy of identifying and counting the maturity of the alfalfa seed pods is improved, and meanwhile, the identifying and counting time is also shortened.

The invention belongs to the technical field of molecular biology, and particularly relates to a molecular marker related to the content of crude protein of medicago sativa and application of the molecular marker. The invention solves the technical problems that the traditional high-protein high-quality alfalfa new variety breeding is single plant selection according to the crude protein content of breeding offspring, and the method is time-consuming, labor-consuming and low in accuracy. The technical scheme of the invention is a molecular marker related to the content of crude protein of medicago sativa. The specific information is as follows: chromosome: chr3; the position is 25995949; sNP (Single Nucleotide Polymorphism) typing: C / A. The invention also designs a primer combination aiming at the molecular marker, and the primer combination can be used for directly and specifically distinguishing and detecting A or C basic groups of SNP mutation sites.

The invention provides bacillus atrophaeus LYZ1127, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the bacillus atrophaeus LYZ1127 is CGMCC (China General Microbiological Culture Collection Center) No.31718, the preservation number of the bacillus atrophaeus LYZ1127 is CGMCC No.31718, and the preservation number of the biocontrol microbial inoculum is CGMCC No.31718. The bacillus atrophaeus LYZ1127 has relatively good bacteriostatic activity on 11 pathogenic bacteria of six common diseases of alfalfa and verticillium dahliae (separated from oxytropis aureocauda plants) belonging to the same genus and different species with verticillium wilt of alfalfa, and pot experiment shows that the bacillus atrophaeus LYZ1127 can promote the growth of alfalfa root systems and plants, can also remarkably reduce the incidence rate of verticillium wilt of alfalfa, and can be applied to the field of agricultural chemical engineering, such as agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering and agricultural chemical engineering. The method has a good control effect on the verticillium wilt of the alfalfa, and provides an important theoretical basis for biological control of the verticillium wilt of the alfalfa.

The invention discloses an alfalfa planting management method based on high-altitude saline-alkali soil, and relates to the technical field of agricultural cultivation, and the method comprises the following steps: S1, an irrigation water preheating step: enabling irrigation water to flow through a deep-color pipeline laid on the ground surface for solar preheating; the preheated irrigation water is used for irrigating the field in a drip irrigation mode, and the irrigation water amount is determined according to the initial salt content of the soil and the target salt leaching depth. According to the alfalfa planting management method based on the high-altitude saline-alkali soil, through combination of irrigation water preheating treatment and precise drip irrigation, the contradiction between salt leaching operation and ground temperature keeping in a high-altitude area is effectively solved. Preheated irrigation water avoids soil temperature sudden drop caused by cold water irrigation, the layered covering technology synergistically inhibits water evaporation and salt upward returning, meanwhile, the soil temperature is kept stable, a proper water temperature and salt environment is created for alfalfa seed germination, and therefore the seedling emergence uniformity and the seedling survival rate are improved.

A novel lettuce variety designated CS33201 is provided. This disclosure thus relates to the seeds of lettuce variety CS33201, to the plants of lettuce variety CS332013, to plant parts of lettuce variety CS33201, to methods for producing a lettuce plant by crossing a plant of lettuce variety CS33201 with a plant of another lettuce variety, and to methods for producing a plant of lettuce variety CS33201 containing in its genetic material one or more backcross conversion traits or transgenes and to the backcross conversion lettuce plants and plant parts produced by those methods.

The present application relates to the technical field of elicitor peptide MsPep and its application, and discloses the use of elicitor peptide MsPep in improving the genetic transformation efficiency of alfalfa, wherein the application takes the leaf of alfalfa as an explant, introduces an exogenous gene through Agrobacterium tumefaciens, and adds the elicitor peptide MsPep in each stage of genetic transformation, so as to significantly improve the dedifferentiation rate, the out-growth rate, the differentiation rate, the out-bud rate, the rooting rate and the transformation efficiency, and shorten the transformation period, and the present application has application prospect in improving the genetic transformation of alfalfa and other plants.