142results about How to "Increased biomass" patented technology

The invention belongs to the technical field of livestock breeding and specifically relates to an ecological method of breeding chicken by planting grass under trees. The method includes that a fine forage grass breed which is shade-tolerant, trampling-resistant, strong in stress resistance and applicable to being planted in the forest is selected, and a forest land for grazing the chicken is modified to form a grassland under the trees for grazing the chicken; a contour grass and shrub zone in a stocking land is used for planting the grass, a chicken house is built in the middle of the grassland under the trees for grazing the chicken, zone division is performed for rotation grazing, and zones are separated by biological fences; and Chinese herbal plants are sowed in rotation grazing zones so that the grazed chicken can freely ingest the plants. A scientific evidence is provided for obvious economic benefits and ecological benefits which are achieved by means of rapid development of domestic fowls and effective ecological rehabilitation, wherein the domestic fowls of Hetian chicken and the like are applicable to stocking in forestland. By means of the model for breeding the chicken, high economic benefits are created, parts of the economic benefits are used for investing development of ecological chicken breeding model, and the created economic benefits and the development of ecological chicken breeding model are combined for creating better benefits, and accordingly, a virtuous cycle is achieved, ecological balance is kept, and the sustainable development is effectively achieved.

The invention discloses a method for rapidly improving plough-layer soil of newly cultivated barren lands in southern hilly and mountainous regions, belonging to the technical field of soil improvement. The method comprises the steps of cultivating the barren lands for preparation; digging latticed fertilizing and planting ditches; preparing and applying mixtures including a fertilizer and a water-retaining agent; sowing seeds of ryegrass for the first time, cradling and covering; sowing seeds of ryegrass next year, cradling and covering; and the like. The method disclosed by the invention is definite in step and simple and feasible in process, after two years continuous integrated improvement, the soil bulk density is reduced, the void fraction is increased, the level of nutritive substances such as organic matters, total nitrogen, quick-acting nitrogen, phosphorus and potassium is improved obviously, the plough-layer soil can reach a medium fertility level, and normal agricultural utilization can be carried out. The method can be popularized and applied in newly cultivated barren lands in southern hilly and mountainous regions of China.

The invention relates to a special controlled release fertilizer for wheat and a preparation method thereof and belongs to the technical field of sustained release fertilizer and controlled release fertilizer. According to the rule of the fertilizer requirement of the wheat, the invention remixes 5 percent, 6 percent and 7 percent thermoplastic resin coated urea, sulfur coated urea, potassium chloride complex fertilizer, the potassium chloride, diammonium phosphate and zinc sulfate exactly to form the special controlled release fertilizer for wheat. The nutrient release rule of the controlled release fertilizer is in accordance with the nutrition absorption rule of the wheat, and the cost of the controlled release fertilizer is reduced; no more fertilization is required after one time of the fertilization of the controlled release fertilizer before the wheat is seeded, which reduces the labor intensity of the farmer and the labor cost.

The invention discloses a method for restoration of high-concentration uranium-polluted soil by water spinach. The method provided by the invention comprises the following steps of refining and flatting uranium-polluted soil, applying 3000 to 4000kg of human and animal manure as base fertilizer to the uranium-polluted soil, carrying out accelerating germination of seeds, carrying out sowing according a sowing ratio of 2.0 to 2.5 kilograms per mu, when rattan has length of 40 to 50 centimeters, harvesting stems and leaves on the ground, transferring the stems and the leaves from the uranium-polluted soil, carrying out concentrated treatment on the stems and the leaves, wherein in harvest, stubble has length of 1 to 2 sections so that regeneration is promoted, when regenerated rattan has length of 40 to 50 centimeters, carrying out harvesting again, wherein harvesting frequency per year is in a range of 4 to 5, and carrying out topdressing after each harvesting so that regenerated seedling growth is promoted. The method provided by the invention has the advantages of simple processes, convenient management, low cost and good restoration effects.

The invention discloses a method for planting corn and cover crops, belongs to the field of crop planting, and particularly relates to a cultivating method of inter-annual crop rotation of corn and the cover crops. The technical problems of reduction of soil quality and non-point source pollution caused by large-scale use of chemical fertilizers, pesticides and herbicides in the corn planting process are solved. The cultivating method comprises the steps of 1, soil preparation in spring, 2, ridging and basal dressing, 3, sowing of corn, 4, sowing of the cover crops on ridges, 5, planting of the cover crops in furrows, 6, harvest of corn, 7, crushing of residual bodies of the cover crops and plowing in the spring of a next year, 8, ridging in the next year, and 9, field management in a second year. The cover crops such as gramineae are planted in the furrows, so that the field space is fully used, and the growth of weeds is avoided. The cover crops such as gramineae are planted in the furrows the biological yield is high, the absorption and utilization capacity for N in deeper layers of soil are good, and the protection effect for water resources and ecological environment is good.

The invention provides a method for expressing and producing pseudoplectania nigrella mature peptide recombinant proteins in pichia pastoris cells in a constitutive type mode, which comprises the following steps of: 1) optimizing pseudoplectania nigrella mature peptide genes; 2) obtaining pichia pastoris glyceraldehyde-3-phosphate dehydrogenase promoter DNA sequences by adopting methods such as molecular cloning and the like, and placing the pseudoplectania nigrella mature peptide genes under the regulation and control of the glyceraldehyde-3-phosphate dehydrogenase promoters to connect with plasmids to obtain new, constitutive and secretory type pichia pastoris efficient expression vectors; 3) converting the pichia pastoris by using the vectors and obtaining the recombinant pichia pastoris strains to obtain the pseudoplectania nigrella mature peptide with bioactivity; and 4) optimizing the high-density fermentation conditions of the engineering strains and the expression and purification of the recombinant protein. The pseudoplectania nigrella mature peptide prepared by the method can be applied in the fields of medicinal treatment, food, feed, scientific research and the like and is suitable for the scale production of pseudoplectania nigrella protein.

The invention discloses a method for utilizing chenopdium album L to restore the soil polluted by high-concentration cesium. The method comprises the steps of flattening and refining the polluted soil, applying 2200-3000kg of thoroughly decomposed excreta of people and animals for each 666.7M<2>, and taking the thoroughly decomposed excreta of people and animals as base fertilizers, planting the chenopdium album L in March to August every year, sowing 100-150g of seeds for each 666.7M<2>, blending fine soils, and sowing uniformly; then keeping 50%-70% of water holding capacity in soil field so as to promote germination and seedling; using a herbicide to removing other plants before the sowing in bare places so as to avoid overshadowing; when the height of the seedling is 50-60cm, harvesting the overground parts, and processing the overground parts intensively; and cleaning the harvesting fields, sowing again, managing and harvesting the chenopdium album L according to the method, thus harvesting the chenopdium album L for three times in one year. According to the method, the operation is simple, the management is convenient, the cost is low and the restoring effect is good.

The invention belongs to the technical field of pesticides and in particular to relates to a multifunctional medicinal plant seed coating agent and preparation and coating methods thereof. The multifunctional medicinal plant seed coating agent comprises active ingredients, auxiliary agents and water. The multifunctional medicinal plant seed coating agent comprises the following raw materials in percentage by mass: the active ingredients: 6-18% of a bactericide, 5-15% of an insecticide, 3-6% of a balancing fertilizer and 0.03-0.04% of a growth regulator; 11-19% of the auxiliary agents; and the balance of water, totaling 100%. According to the technical scheme, the invention also provides the preparation method of the coating agent. The method is simple and feasible and easy to be industrially popularized.

The invention discloses a novel Hericium erinaceus bacterial strain 1 of Hericium erinaceus, which has the advantages that: the biological yield of the novel Hericium erinaceus mycelium in the Hericium erinaceus bacterial strain 1 which is obtained in a culture method disclosed by the invention is high and can reach 12g/L which is twice of the fermentation production capacity in present factories; and the growth speed of the novel Hericium erinaceus bacterial strain 1 is fast and can reach 5.8mm/d, and the yield is high.

The invention belongs to the technical field of plant molecular breeding and biology, and discloses application of a PLD (phospholipase D) epsilon gene in increasing nitrogen nutrition effective utilization and seed yield of crops. The nucleotide sequence of the PLD epsilon gene is shown as SEQ ID NO:1, or has a similarity more than or equal to 60 percent as the nucleotide sequence shown as SEQ ID NO:1. The invention also discloses a method for increasing nitrogen nutrition effective utilization and seed yield of rape. Tests prove that growth and occurrence of lateral roots of rape can be promoted due to overexpression of AtPLD epsilon either in nitrogen starvation or nitrogen sufficiency condition, increase of rape leaf tissue cell growth and biological yield can be reinforced, absorption transportation and assimilation metabolism processes of rape plants can be promoted, which are particularly indicated in that the expression quantities of genes NTR1.1 and NTR2.1 related to overexpressed plant N transportation are remarkably higher than that of a wild type, and the nitrogen absorption rate is remarkably higher than that of the wild type.

The invention discloses a forage hedgerow soil conservation planting technology, comprising the following steps of: tillage and land preparation, seeding and seedling, and plantation. The technology has the following advantages that: the forage hedgerow can prominently reduce sediment loss amount and runoff and has good effect of fast controlling water and soil loss; the forage hedgerow promotes value-cost ratio of energy of the entire system and becomes an effective measure to enhance the agricultural efficiency in hilly areas; bean forage hedgerow can offer excellent shelter to ground spider so as to enrich the diversity of spiders in farmland. The technology not only focuses on ecological and social benefits, but can also create economical benefit, in addition, the technology realizes sustainable agricultural development on dry hillside fields, effectually controls water and soil loss of dry hillside farmlands, enriches soil fertility, enhances grain yield and can also produce a certain amount of high-grade forage to further guarantee the supply of partial feed for the development of animal husbandry in agricultural regions.

The invention discloses a seed cold induction device and method based on an ultrasonic wave technology. The seed cold induction device includes a refrigeration module, a control module and a power supply module; the refrigeration module includes a two-stage refrigeration system and an inner box, and the two-stage refrigeration system includes a compression refrigerating machine and temperature difference refrigerating sheets; the inner box includes a heat insulation cover, a temperature-controllable box body, ultrasonic wave energy converters, coils, a stirrer and concentric drain caps; the control module includes a single-ship computer, a drive circuit and an ultrasonic wave generator; the inner wall face of the side, close to low-temperature anti-freezing liquid, of the temperature-controllable box body is a surface with gradient surface energy, and the inner wall face of the side, close to the temperature difference refrigerating sheets, of the temperature-controllable box body is of a multi-scale groove structure. According to the seed cold induction device and method based on the ultrasonic wave technology, ultrasonic waves are adopted to stimulate seeds, the germination rateof the seeds is increased, the germination potential of the seeds is improved, and the growth of seedlings is promoted; by adopting the cavitation and mass transfer effects of the ultrasonic waves, the configuration of enzyme molecules of the seeds is changed, the activity of enzymes is improved, the stress-resistant, antiviral and oxidation-resistant capabilities of the seeds are improved, and the biological yield is increased.

The invention discloses an isopentenyl isoquinoline alkaloid compound as well as a preparation method and application thereof. The compound is obtained by subjecting a whole plant of Thalictrum cirrhosum H.Lev. adopted as a raw material to extraction with ethyl acetate, silica gel column chromatography and high-pressure liquid chromatography separation and purification. The molecular formula of the compound is C18H21NO3, and the compound has the following structural formula as shown in the specification. The compound is named as 3-hydroxy-1-(6-methoxy-7-isopentenyl isoquinolin-1-yl)-propan-1-one. The inhibition effect of the compound on Phytophthora nicotianae is remarkably superior to that of agricultural streptomycin, and the prevention and treatment effect on tobacco black shank reaches(65.7 +/- 3.0)%. The raw material is widely distributed, high in biological yield, and high in alkaloid content, the compound preparation process is simple, the production cost is low, and the compound is prone to industrial application.

The invention relates to the field of marine microalgae, in particular to a heterotrophic culture medium used for large-scale cultivation of marine microalgae. The culture medium is prepared from 0.5-1.5 g of nitrate, 2-10 mg of monopotassium phosphate, 2-10 mg of dipotassium phosphate, 0.1-1 mg of ferric citrate, 0.5-2 mg of magnesium sulfate heptahydrate, 0.5-2 mg of calcium chloride dehydrate, 5-20 g of an organic carbon source and 1000 mL of filtered seawater. The nitrate is potassium nitrate or sodium nitrate, and the organic carbon source is glucose or sucrose. The heterotrophic culture medium is widely used for the large-scale cultivation of the marine microalgae, can increase growth velocity of the marine microalgae, shorten the growth cycle of the marine microalgae, increase the biological yield of the marine microalgae, meanwhile sharply reduce the cost of the culture medium, and is an ideal culture medium used for the large-scale cultivation.

The invention provides a breeding method of entire silo corn hybrids. The breeding method comprises the steps of breeding a parent selfing line, isolating spatially, artificially hybridizing, bagging for isolation and selfing for achieving homozygosity; performing test mating on a selfing line A and a selfing line B to obtain a single cross hybrid F1; and finally, carrying out test mating on the single cross hybrid F1 used as a female parent and a third selfing line C used as a male parent to breed the entire silo corn hybrid (three-way cross hybrid). The breeding method adopts three selfing lines to breed the entire silo corn hybrid and opens up a new method for breeding of the silo corn hybrid; the bred three-way cross hybrid has the characteristics of high quality, high biological yield and high seed production, contains more than 10% of crude protein, lower than 45% of neutral detergent fibers and lower than 25% of acid detergent fibers; two selfing lines are subjected to test mating to obtain the single cross hybrid, then the single cross hybrid used as the female parent and another one selfing line used as the male parent are used for seed production, and therefore, the seed production yield is ensured and the seed production cost can be reduced.

The invention discloses an under-forest konjak annual plantation method comprising following steps: (1) forest land selecting; (2)wild konjak ecological environment imitating; (3) forest land reclaiming; (4) konjak cormlet selecting; (5) konjak cormlet disinfecting; (6) sowing; (7) weeding; (8) konjak planted forest land overwintering; (9) harvesting. The method adopts under-forest plantation of konjak to realize the original ecological planting from the forest to the forest, and creates the habitat close to the origin of the konjak, satisfies the requirement on external environment for konjak; the purposes of healthy planting and healthy growth are fulfilled. The method of under-forest plantation of konjak has advantages such as good growth condition, low water content in seed konjak, and safe overwintering in forest land; the konjak can be directly transplanted in field in the second spring, the seed konjak has good quality, low bacterium, and high emergence rate; there is no need to harvest seed konjak in the autumn and to winter indoor, which is labor saving and reduces production processes.

The method of utilizing fuel coal desulfurizing product in improving alkali soil includes first sampling soil; testing and calculating Na2CO3, NaHCO3, Mg(HCO3)2, Na and Mg amount to be increased or decreased and molar CaSO4 amount needed for improvement based on the formulae; calculating the needed desulfurizing product amount according to the desulfurizing product components; and applying the desulfurizing product to the surface of soil and irrigation. Compared with available technology, the present invention has the advantages of fast improving speed and high improving effect.

The invention belongs to the technical field of green manure plowing field-returning and discloses a method for Dongmu No.70 green manure plowing field-returning in a northern cold tobacco growing area. According to the YC/T142-1998 Investigation Methods of Tobacco Agronomic Characters, main agronomic characters of tobacco plants in each field are measured; grading and then statistics of tobacco yields, mean prices, output values and proportions of high, medium and low grade tobacco are carried out according to standard requirements in the GB 2635-1992 Flue-cured Tobacco, and main economic characters are investigated; field survey and sampling are carried out for tobacco in different plots, and C3F tobacco is extracted in processing and delivered to the Tobacco Quality Supervision and Inspection Test Center of the Ministry of Agriculture for testing and assessment; in harvesting of tobacco uppermost leaves is realized by a cutting-ring method, and a relative water content and a volumeweight of soil are measured; and soil organic matters are measured by a potassium dichromate volumetric method-an external heating method, and the content of water soluble chlorine ions is measured bya silver nitrate titration method. The method applies a green manure plowing technology for the tobacco area through green manure variety tests, and provides a theoretical basis for improving soil, fertility and tobacco quality.

The invention belongs to the field of biological control of crop diseases, and provides application of cis-abienol in control of bacterial wilt and application of a tobacco labdanum compound in control of bacterial wilt directed at the problems of unsatisfactory control effect, environmental pollution and food safety in existing crop bacterial wilt control technology. According to the invention, bacterial wilt is controlled by improving the resistance of crops, and compared with currently reported biological source preparations, the labdanum compound has the characteristics of low effective concentration, high control effect, no toxicity, durability and safety.

The invention provides a method for quickly planting fracturing alkali soil switchgrass, which mainly aims to solve problems such as high alkalization degree, heavy texture and low fertility of fracturing alkali soil, low emergence rate of switchgrass and difficult planting. The technical scheme adopted by the method for quickly planting the fracturing alkali soil switchgrass, provided by the invention, comprises the following steps of: 1, laser soil leveling; 2, improved material application; 3, fertilizing in a composite organic and inorganic way; 4, deeply tilling and upturned soil sunning; 5, irrigating, salt leaching and alkali pressing; 6, soil preparing before transplantation; 7, seedling transplantation; 8, field management; 9, timely harvesting; 10, winter irrigating, alkali pressing and soil moisture conservation. According to the method for quickly planting the alkali soil switchgrass, disclosed by the invention, the quick planting of the switchgrass on the fracturing alkali soil can be realized, the switchgrass can well grow, a higher biological yield can be obtained at the same year when the switchgrass is planted, the method is suitable for planting the fracturing alkali soil switchgrass on Yinchuan Plain of Ningxia, the method is beneficial for resource development and utilization and biomass energy production, and obvious economic, social and ecological benefits are obtained.