143results about How to "Increased biomass" patented technology

The invention belongs to the technical field of livestock breeding and specifically relates to an ecological method of breeding chicken by planting grass under trees. The method includes that a fine forage grass breed which is shade-tolerant, trampling-resistant, strong in stress resistance and applicable to being planted in the forest is selected, and a forest land for grazing the chicken is modified to form a grassland under the trees for grazing the chicken; a contour grass and shrub zone in a stocking land is used for planting the grass, a chicken house is built in the middle of the grassland under the trees for grazing the chicken, zone division is performed for rotation grazing, and zones are separated by biological fences; and Chinese herbal plants are sowed in rotation grazing zones so that the grazed chicken can freely ingest the plants. A scientific evidence is provided for obvious economic benefits and ecological benefits which are achieved by means of rapid development of domestic fowls and effective ecological rehabilitation, wherein the domestic fowls of Hetian chicken and the like are applicable to stocking in forestland. By means of the model for breeding the chicken, high economic benefits are created, parts of the economic benefits are used for investing development of ecological chicken breeding model, and the created economic benefits and the development of ecological chicken breeding model are combined for creating better benefits, and accordingly, a virtuous cycle is achieved, ecological balance is kept, and the sustainable development is effectively achieved.

The invention discloses a liquid submerged fermentation culture method for hericium erinaceus, and belongs to the field of culture methods for edible fungi. The culture method comprises the following steps of: activating hericium erinaceus strains on a slant culture medium, then performing primary strain culture on a liquid seed culture medium, performing secondary strain culture on a liquid fermentation culture medium, and finally performing amplified culture on the liquid fermentation culture medium to obtain hericium erinaceus mycelium. The invention also discloses hedgehog hydnum 1 in the hericium erinaceus strains, a seed culture medium and a fermentation culture medium for hericium erinaceus culture. The biological yield of the hericium erinaceus mycelium obtained by the culture method is high and can reach 12g / L which is over two times as much as the fermentation production level of the current factory; the hedgehog hydnum 1 in the hericium erinaceus strains has high growth speed which can reach 5.8 millimeters every day; and moreover, the liquid seed culture medium and the fermentation culture medium have good culture effect so that the hericium erinaceus mycelium has quick growth and high yield.

The invention relates to a soilless culture trough and an application thereof, and belongs to soilless culture equipment. The soilless culture trough comprises a foamed plastic trough body and is characterized in that a plastic film is lined on the inner wall of the trough body, river sand is filled at the lower part of the trough body, a culture medium is arranged at the upper part of the trough body, a piece of braid shield fabric is arranged between the river sand and the culture medium, a vent pipe is buried at the bottom of the river sand, a piece of non-woven fabric wraps the outer wall of the vent pipe, one end of the vent pipe extends out of the trough body, the other end of the vent pipe is closed, and air vents are formed in the wall of the vent pipe. The purpose of uniform air outgoing of the vent pipe is realized by using a simple method in which the non-woven fabric and the sand are adopted, and the cost is low. Problems in four major medium soilless culture planting processes, including medium warming, medium ventilation, medium drainage and medium sterilization, are solved by one set of vent pipe, and one pipe can be used for multiple purposes.

The invention discloses a method for rapidly improving plough-layer soil of newly cultivated barren lands in southern hilly and mountainous regions, belonging to the technical field of soil improvement. The method comprises the steps of cultivating the barren lands for preparation; digging latticed fertilizing and planting ditches; preparing and applying mixtures including a fertilizer and a water-retaining agent; sowing seeds of ryegrass for the first time, cradling and covering; sowing seeds of ryegrass next year, cradling and covering; and the like. The method disclosed by the invention is definite in step and simple and feasible in process, after two years continuous integrated improvement, the soil bulk density is reduced, the void fraction is increased, the level of nutritive substances such as organic matters, total nitrogen, quick-acting nitrogen, phosphorus and potassium is improved obviously, the plough-layer soil can reach a medium fertility level, and normal agricultural utilization can be carried out. The method can be popularized and applied in newly cultivated barren lands in southern hilly and mountainous regions of China.

The invention relates to a special controlled release fertilizer for wheat and a preparation method thereof and belongs to the technical field of sustained release fertilizer and controlled release fertilizer. According to the rule of the fertilizer requirement of the wheat, the invention remixes 5 percent, 6 percent and 7 percent thermoplastic resin coated urea, sulfur coated urea, potassium chloride complex fertilizer, the potassium chloride, diammonium phosphate and zinc sulfate exactly to form the special controlled release fertilizer for wheat. The nutrient release rule of the controlled release fertilizer is in accordance with the nutrition absorption rule of the wheat, and the cost of the controlled release fertilizer is reduced; no more fertilization is required after one time of the fertilization of the controlled release fertilizer before the wheat is seeded, which reduces the labor intensity of the farmer and the labor cost.

The invention discloses a method for producing a docosahexaenoic acid (DHA) by using schizochytrium limacinum. The method comprises the following steps of (1) activating and culturing schizochytrium limacinum into a schizochytrium limacinum seed solution; (2) introducing the schizochytrium limacinum seed solution into a fermentation tank for culturing under the culture condition of 25-28 DEG C, aerating and stirring at a constant rotation speed, meanwhile fluidly feeding materials including carbon sources, nitrogen sources and glycerin, and keeping the concentration of glycerin at 0-10.0 g / liter; (3) controlling the temperature of a fermentation stable period at 18-23 DEG C, aerating and stirring at a constant rotation speed; and (4) when the concentration of the carbon source in a fermentation liquor is less than 5.0 g / liter, completing fermentation and collecting wet cells to obtain docosahexaenoic acid-rich greases. After the method is applied to large-scale fermentation and production of docosahexaenoic acid, the biomass of schizochytrium limacinum and the DHA content can increase, the quality of a DHA grease product is greatly improved, the grease quality is further improved and the energy consumption and the production cost are reduced.

The invention relates to a double-layered growing method for lucern in mountainous areas of arid regions. The method is characterized in that: grandifoliate lucern is adopted as a main grown variety, an annual associated plant variety, such as rape, oat or highland barley, is chosen, and by means of a orthogonal test design and sowing order (position), growing spacing, growing depth, amount of applied fertilizer and other technical measures, and by utilizing the concealing and protectin effect generated by the double-layered growing method for the lucern in mountainous areas on lucern seedlings, the double-layered growing method can increase the current survival rate of the lucern seedlings. The double-layered growing method can promote the success of desert grassland improvement and artificial pasture construction under the environmental conditions of arid mountainous areas, becomes an optimal technology portfolio for growing lucern at medium and high altitudes in mountainous areas of arid regions, and greatly increases the survival rate of lucern in desert grassland restoration and artificial pasture construction and the yield of currently grown lucern in mountainous areas of arid regions at the altitude of 3000 to 1800 meters.

The invention discloses a method for restoration of high-concentration uranium-polluted soil by water spinach. The method provided by the invention comprises the following steps of refining and flatting uranium-polluted soil, applying 3000 to 4000kg of human and animal manure as base fertilizer to the uranium-polluted soil, carrying out accelerating germination of seeds, carrying out sowing according a sowing ratio of 2.0 to 2.5 kilograms per mu, when rattan has length of 40 to 50 centimeters, harvesting stems and leaves on the ground, transferring the stems and the leaves from the uranium-polluted soil, carrying out concentrated treatment on the stems and the leaves, wherein in harvest, stubble has length of 1 to 2 sections so that regeneration is promoted, when regenerated rattan has length of 40 to 50 centimeters, carrying out harvesting again, wherein harvesting frequency per year is in a range of 4 to 5, and carrying out topdressing after each harvesting so that regenerated seedling growth is promoted. The method provided by the invention has the advantages of simple processes, convenient management, low cost and good restoration effects.

The invention discloses a method for restoring wet land and mud-flat soil polluted by high-concentration cesium. The method comprises the steps of transplanting 100 sections of subterraneous stems and roots of alternanthera philoxeroides for each square meter of wet land and mud-flat soil polluted, and keeping more than 80% of water holding capacity in soil fields; harvesting and intensively processing overground parts of the plants when the main stems are about 50cm; in harvesting, reserving 1-2 sections of stubbles so as to be beneficial to regeneration, and harvesting and intensively processing the plants again when the main stems of the seedlings are about 50cm; harvesting the plants for three times in one year, and harvesting for a plurality of years; and applying a compound fertilizer additionally in 15-20 days after the seedlings are transplanted or harvested. According to the method, the operation is simple, the management is convenient, the cost is low, and the restoring effect is good.

The invention discloses a method for planting corn and cover crops, belongs to the field of crop planting, and particularly relates to a cultivating method of inter-annual crop rotation of corn and the cover crops. The technical problems of reduction of soil quality and non-point source pollution caused by large-scale use of chemical fertilizers, pesticides and herbicides in the corn planting process are solved. The cultivating method comprises the steps of 1, soil preparation in spring, 2, ridging and basal dressing, 3, sowing of corn, 4, sowing of the cover crops on ridges, 5, planting of the cover crops in furrows, 6, harvest of corn, 7, crushing of residual bodies of the cover crops and plowing in the spring of a next year, 8, ridging in the next year, and 9, field management in a second year. The cover crops such as gramineae are planted in the furrows, so that the field space is fully used, and the growth of weeds is avoided. The cover crops such as gramineae are planted in the furrows the biological yield is high, the absorption and utilization capacity for N in deeper layers of soil are good, and the protection effect for water resources and ecological environment is good.

The invention provides a method for expressing and producing pseudoplectania nigrella mature peptide recombinant proteins in pichia pastoris cells in a constitutive type mode, which comprises the following steps of: 1) optimizing pseudoplectania nigrella mature peptide genes; 2) obtaining pichia pastoris glyceraldehyde-3-phosphate dehydrogenase promoter DNA sequences by adopting methods such as molecular cloning and the like, and placing the pseudoplectania nigrella mature peptide genes under the regulation and control of the glyceraldehyde-3-phosphate dehydrogenase promoters to connect with plasmids to obtain new, constitutive and secretory type pichia pastoris efficient expression vectors; 3) converting the pichia pastoris by using the vectors and obtaining the recombinant pichia pastoris strains to obtain the pseudoplectania nigrella mature peptide with bioactivity; and 4) optimizing the high-density fermentation conditions of the engineering strains and the expression and purification of the recombinant protein. The pseudoplectania nigrella mature peptide prepared by the method can be applied in the fields of medicinal treatment, food, feed, scientific research and the like and is suitable for the scale production of pseudoplectania nigrella protein.

The invention discloses a method for utilizing chenopdium album L to restore the soil polluted by high-concentration cesium. The method comprises the steps of flattening and refining the polluted soil, applying 2200-3000kg of thoroughly decomposed excreta of people and animals for each 666.7M<2>, and taking the thoroughly decomposed excreta of people and animals as base fertilizers, planting the chenopdium album L in March to August every year, sowing 100-150g of seeds for each 666.7M<2>, blending fine soils, and sowing uniformly; then keeping 50%-70% of water holding capacity in soil field so as to promote germination and seedling; using a herbicide to removing other plants before the sowing in bare places so as to avoid overshadowing; when the height of the seedling is 50-60cm, harvesting the overground parts, and processing the overground parts intensively; and cleaning the harvesting fields, sowing again, managing and harvesting the chenopdium album L according to the method, thus harvesting the chenopdium album L for three times in one year. According to the method, the operation is simple, the management is convenient, the cost is low and the restoring effect is good.

The invention belongs to the technical field of pesticides and in particular to relates to a multifunctional medicinal plant seed coating agent and preparation and coating methods thereof. The multifunctional medicinal plant seed coating agent comprises active ingredients, auxiliary agents and water. The multifunctional medicinal plant seed coating agent comprises the following raw materials in percentage by mass: the active ingredients: 6-18% of a bactericide, 5-15% of an insecticide, 3-6% of a balancing fertilizer and 0.03-0.04% of a growth regulator; 11-19% of the auxiliary agents; and the balance of water, totaling 100%. According to the technical scheme, the invention also provides the preparation method of the coating agent. The method is simple and feasible and easy to be industrially popularized.

The invention discloses a novel Hericium erinaceus bacterial strain 1 of Hericium erinaceus, which has the advantages that: the biological yield of the novel Hericium erinaceus mycelium in the Hericium erinaceus bacterial strain 1 which is obtained in a culture method disclosed by the invention is high and can reach 12g / L which is twice of the fermentation production capacity in present factories; and the growth speed of the novel Hericium erinaceus bacterial strain 1 is fast and can reach 5.8mm / d, and the yield is high.

The invention belongs to the technical field of plant molecular breeding and biology, and discloses application of a PLD (phospholipase D) epsilon gene in increasing nitrogen nutrition effective utilization and seed yield of crops. The nucleotide sequence of the PLD epsilon gene is shown as SEQ ID NO:1, or has a similarity more than or equal to 60 percent as the nucleotide sequence shown as SEQ ID NO:1. The invention also discloses a method for increasing nitrogen nutrition effective utilization and seed yield of rape. Tests prove that growth and occurrence of lateral roots of rape can be promoted due to overexpression of AtPLD epsilon either in nitrogen starvation or nitrogen sufficiency condition, increase of rape leaf tissue cell growth and biological yield can be reinforced, absorption transportation and assimilation metabolism processes of rape plants can be promoted, which are particularly indicated in that the expression quantities of genes NTR1.1 and NTR2.1 related to overexpressed plant N transportation are remarkably higher than that of a wild type, and the nitrogen absorption rate is remarkably higher than that of the wild type.

The invention relates to an apparatus for collecting root secretions through an aerosol culture process, and a collecting method thereof, and belongs to the technical field of the agriculture engineering. The apparatus for collecting the root secretions through the aerosol culture process is formed by a culture box, a planting bowl, a nutritional solution box, a collecting bottle, a liquid storage bottle and a resin column, the planting bowl is installed on the top cover of the culture box, the nutritional solution box is provided with an ultrasonic atomizer and a supply pump, the collecting bottle is fixed on the net plate at the top of the nutritional solution box, the bottleneck of the collecting bottle is provided with a collecting funnel, the collecting bottle is connected with the liquid storage bottle, and the liquid storage bottle is connected with the resin column. The collecting method comprises the following steps: selecting plant seedlings, planting in the planting bowl, carrying out atomization injection of a nutritional liquid on plant roots, and culturing until harvesting. The detection data of the plant root secretions in the invention are accurate and precise, and the method is especially suitable for scientific research workers to research the ecology of the plant roots, the root nutrients and the relevant cultivation technology of the roots through utilizing the apparatus.

The invention discloses a forage hedgerow soil conservation planting technology, comprising the following steps of: tillage and land preparation, seeding and seedling, and plantation. The technology has the following advantages that: the forage hedgerow can prominently reduce sediment loss amount and runoff and has good effect of fast controlling water and soil loss; the forage hedgerow promotes value-cost ratio of energy of the entire system and becomes an effective measure to enhance the agricultural efficiency in hilly areas; bean forage hedgerow can offer excellent shelter to ground spider so as to enrich the diversity of spiders in farmland. The technology not only focuses on ecological and social benefits, but can also create economical benefit, in addition, the technology realizes sustainable agricultural development on dry hillside fields, effectually controls water and soil loss of dry hillside farmlands, enriches soil fertility, enhances grain yield and can also produce a certain amount of high-grade forage to further guarantee the supply of partial feed for the development of animal husbandry in agricultural regions.

The invention discloses a seed cold induction device and method based on an ultrasonic wave technology. The seed cold induction device includes a refrigeration module, a control module and a power supply module; the refrigeration module includes a two-stage refrigeration system and an inner box, and the two-stage refrigeration system includes a compression refrigerating machine and temperature difference refrigerating sheets; the inner box includes a heat insulation cover, a temperature-controllable box body, ultrasonic wave energy converters, coils, a stirrer and concentric drain caps; the control module includes a single-ship computer, a drive circuit and an ultrasonic wave generator; the inner wall face of the side, close to low-temperature anti-freezing liquid, of the temperature-controllable box body is a surface with gradient surface energy, and the inner wall face of the side, close to the temperature difference refrigerating sheets, of the temperature-controllable box body is of a multi-scale groove structure. According to the seed cold induction device and method based on the ultrasonic wave technology, ultrasonic waves are adopted to stimulate seeds, the germination rateof the seeds is increased, the germination potential of the seeds is improved, and the growth of seedlings is promoted; by adopting the cavitation and mass transfer effects of the ultrasonic waves, the configuration of enzyme molecules of the seeds is changed, the activity of enzymes is improved, the stress-resistant, antiviral and oxidation-resistant capabilities of the seeds are improved, and the biological yield is increased.

The invention discloses an isopentenyl isoquinoline alkaloid compound as well as a preparation method and application thereof. The compound is obtained by subjecting a whole plant of Thalictrum cirrhosum H.Lev. adopted as a raw material to extraction with ethyl acetate, silica gel column chromatography and high-pressure liquid chromatography separation and purification. The molecular formula of the compound is C18H21NO3, and the compound has the following structural formula as shown in the specification. The compound is named as 3-hydroxy-1-(6-methoxy-7-isopentenyl isoquinolin-1-yl)-propan-1-one. The inhibition effect of the compound on Phytophthora nicotianae is remarkably superior to that of agricultural streptomycin, and the prevention and treatment effect on tobacco black shank reaches(65.7 + / - 3.0)%. The raw material is widely distributed, high in biological yield, and high in alkaloid content, the compound preparation process is simple, the production cost is low, and the compound is prone to industrial application.

The invention relates to the field of marine microalgae, in particular to a heterotrophic culture medium used for large-scale cultivation of marine microalgae. The culture medium is prepared from 0.5-1.5 g of nitrate, 2-10 mg of monopotassium phosphate, 2-10 mg of dipotassium phosphate, 0.1-1 mg of ferric citrate, 0.5-2 mg of magnesium sulfate heptahydrate, 0.5-2 mg of calcium chloride dehydrate, 5-20 g of an organic carbon source and 1000 mL of filtered seawater. The nitrate is potassium nitrate or sodium nitrate, and the organic carbon source is glucose or sucrose. The heterotrophic culture medium is widely used for the large-scale cultivation of the marine microalgae, can increase growth velocity of the marine microalgae, shorten the growth cycle of the marine microalgae, increase the biological yield of the marine microalgae, meanwhile sharply reduce the cost of the culture medium, and is an ideal culture medium used for the large-scale cultivation.

The invention provides a breeding method of entire silo corn hybrids. The breeding method comprises the steps of breeding a parent selfing line, isolating spatially, artificially hybridizing, bagging for isolation and selfing for achieving homozygosity; performing test mating on a selfing line A and a selfing line B to obtain a single cross hybrid F1; and finally, carrying out test mating on the single cross hybrid F1 used as a female parent and a third selfing line C used as a male parent to breed the entire silo corn hybrid (three-way cross hybrid). The breeding method adopts three selfing lines to breed the entire silo corn hybrid and opens up a new method for breeding of the silo corn hybrid; the bred three-way cross hybrid has the characteristics of high quality, high biological yield and high seed production, contains more than 10% of crude protein, lower than 45% of neutral detergent fibers and lower than 25% of acid detergent fibers; two selfing lines are subjected to test mating to obtain the single cross hybrid, then the single cross hybrid used as the female parent and another one selfing line used as the male parent are used for seed production, and therefore, the seed production yield is ensured and the seed production cost can be reduced.

The invention relates to a method for improving salt stress tolerance of medicago truncatula. The method comprises the steps: (1) preparing a solid CM complete medium for piriformospora indica; (2) culturing the piriformospora indica and collecting a spore suspension; (3) inoculating the piriformospora indica to the medicago truncatula; and (4) transplanting medicago truncatula seedlings and carrying out later-stage management. According to the method, the piriformospora indica spore suspension is adopted to soak seeds in advance, so that the piriformospora indica can be parasitized to surfaces of the medicago truncatula seeds, the seeds are promoted to germinate ahead of time, and the germination percentage of the seeds is remarkably increased (increased to 95.0% from the original 80.0%); the biological yield of the medicago truncatula can be remarkably increased; and through parasitizing the piriformospora indica to roots of the medicago truncatula, the accumulation of sodium ions in the roots of the medicago truncatula can be lowered, and thus the salt stress tolerance of the medicago truncatula is improved.

The invention belongs to the technical field of transcription factors, and discloses a xanthoceras sorbifolia bunge drought induction transcription factor XsMYB308L and application thereof. The invention relates to a xanthoceras sorbifolia bunge drought induction transcription factor XsMYB308L, which has a nucleotide sequence as shown in SEQ ID NO.1. The invention also provides a protein coded by the xanthoceras sorbifolia bunge drought induction transcription factor XsMYB308L, and the protein has an amino acid sequence shown as SEQ ID NO.2. The invention further provides a pair of primers for amplifying the xanthoceras sorbifolia bunge drought induction transcription factor XsMYB308L. The pair of primers has a nucleotide sequence as shown in SEQ ID NO.3. The XsMYB308L gene provided by the invention has important significance in cultivating transgenic crops of drought-resistant varieties and improving the stress resistance of the crops.

The invention discloses an under-forest konjak annual plantation method comprising following steps: (1) forest land selecting; (2)wild konjak ecological environment imitating; (3) forest land reclaiming; (4) konjak cormlet selecting; (5) konjak cormlet disinfecting; (6) sowing; (7) weeding; (8) konjak planted forest land overwintering; (9) harvesting. The method adopts under-forest plantation of konjak to realize the original ecological planting from the forest to the forest, and creates the habitat close to the origin of the konjak, satisfies the requirement on external environment for konjak; the purposes of healthy planting and healthy growth are fulfilled. The method of under-forest plantation of konjak has advantages such as good growth condition, low water content in seed konjak, and safe overwintering in forest land; the konjak can be directly transplanted in field in the second spring, the seed konjak has good quality, low bacterium, and high emergence rate; there is no need to harvest seed konjak in the autumn and to winter indoor, which is labor saving and reduces production processes.

This invention relates to a new usage of desulfurized wastes of coal smoke and a method for improving alkali earth, in which, the method includes: sampling an earth-sample to test it and computing the volume of Na2CO3, NaHCO3, Mg(HCO3)2,+)Na and Mg, computing the mol volume of CaSO4 necessary for the reform then computing the necessary volume for getting the smoke desulfurized wastes based on their composition to spread the wastes on the earth's surface and irrigate it and applying the field management measure same to the ordinary earth.

The method of utilizing fuel coal desulfurizing product in improving alkali soil includes first sampling soil; testing and calculating Na2CO3, NaHCO3, Mg(HCO3)2, Na and Mg amount to be increased or decreased and molar CaSO4 amount needed for improvement based on the formulae; calculating the needed desulfurizing product amount according to the desulfurizing product components; and applying the desulfurizing product to the surface of soil and irrigation. Compared with available technology, the present invention has the advantages of fast improving speed and high improving effect.

The invention belongs to the technical field of green manure plowing field-returning and discloses a method for Dongmu No.70 green manure plowing field-returning in a northern cold tobacco growing area. According to the YC / T142-1998 Investigation Methods of Tobacco Agronomic Characters, main agronomic characters of tobacco plants in each field are measured; grading and then statistics of tobacco yields, mean prices, output values and proportions of high, medium and low grade tobacco are carried out according to standard requirements in the GB 2635-1992 Flue-cured Tobacco, and main economic characters are investigated; field survey and sampling are carried out for tobacco in different plots, and C3F tobacco is extracted in processing and delivered to the Tobacco Quality Supervision and Inspection Test Center of the Ministry of Agriculture for testing and assessment; in harvesting of tobacco uppermost leaves is realized by a cutting-ring method, and a relative water content and a volumeweight of soil are measured; and soil organic matters are measured by a potassium dichromate volumetric method-an external heating method, and the content of water soluble chlorine ions is measured bya silver nitrate titration method. The method applies a green manure plowing technology for the tobacco area through green manure variety tests, and provides a theoretical basis for improving soil, fertility and tobacco quality.

The invention belongs to the field of biological control of crop diseases, and provides application of cis-abienol in control of bacterial wilt and application of a tobacco labdanum compound in control of bacterial wilt directed at the problems of unsatisfactory control effect, environmental pollution and food safety in existing crop bacterial wilt control technology. According to the invention, bacterial wilt is controlled by improving the resistance of crops, and compared with currently reported biological source preparations, the labdanum compound has the characteristics of low effective concentration, high control effect, no toxicity, durability and safety.