163 results about "Agricultural development" patented technology

The invention discloses a SbWRKY51 gene, a promoter and application of the SbWRKY51 gene and the promoter in improvement of salt tolerance of sorghum, and belongs to the technical field of plant genetic engineering. The SbWRKY51 gene is screened from sorghum, and the expression level of the SbWRKY51 gene is up-regulated after salt stress treatment. The protein coded by the gene belongs to a WRKY transcription factor family, has transcriptional activation activity, and can start the expression of a reporter gene. Sorghum overexpression strains and complementary strains of the gene are obtained, salt tolerance related physiological indexes are measured, overexpression of the gene can improve the seed germination rate, increase the seed root length, regulate ion balance and enhance the free radical scavenging capacity of plants, and the effect of the gene in the aspect of improving the salt tolerance is embodied. A further research finds that the SbWRKY51 gene can enhance the plant salt tolerance by regulating a lignin synthesis pathway. The technical scheme provides a basis for cultivating resistant plants, and has important guiding significance for enhancing the production potential of sorghum under a high-salt condition and promoting agricultural development.

The invention provides a territorial space planning index dynamic evaluation system, which comprises the following steps of: determining index data for dynamic evaluation in territorial space planning, and acquiring and preprocessing the index data; analyzing and evaluating the change trend of the index data by using a time sequence analysis method; and setting a visual information display module, setting an early warning threshold value, synchronously completing real-time display of current situation information, analysis and evaluation information and early warning information of the index data, and completing dynamic evaluation operation. The method comprises the following steps: firstly, determining index data of dynamic evaluation in territorial space planning, the index data comprising an ecological protection direction, an agricultural development direction, an urban and rural development direction and an infrastructure direction; static-to-dynamic adjustment of evaluation of territorial space planning is realized, integration, analysis and evaluation of index data are facilitated, full-cycle dynamic monitoring of territorial space planning indexes can be completed, and territorial space planning operation can be completed efficiently.

The invention discloses application of leguminous green manure in preparation of a multifunctional soil conditioner for improving soil enzyme activity, relieving soil microbial metabolism limitation and / or enhancing a soil ecosystem. The leguminous green manure is small crown flowers. By means of the controllable decomposition characteristic and specific secretions, the activity of soil carbon (BG and CBH), nitrogen (NAG and LAP) and phosphorus (AKP) circulation key enzymes can be remarkably enhanced, the activity of the AKP is improved by 406%, the activity of the BG is improved by 158%, the microbial carbon limitation is effectively relieved by 12%, and the resource utilization strategy is optimized. The microcelosia cristata treatment can synchronously improve the soil ecological multi-functional index (EMF) and improve the nutrient circulation synergistic efficiency. Compared with the traditional leguminous green manure, the method has the advantages that the improvement effect of the microcelosia cristata in the degraded orchard is more continuous and ecologically adaptive, and an effective and feasible new soil remediation way is provided for agricultural sustainable development.

The invention discloses application of cordyceps javanica Bd01 and a dispersible oil suspending agent thereof to prevention and control of spodoptera frugiperda, and relates to the technical field of biology. The research finds that the cordyceps javanica Bd01 has a strong infection lethal effect on larvae of spodoptera frugiperda at all ages, the pupation rate and eclosion rate of the larvae can be remarkably reduced, and the breeding cycle of pests can be effectively blocked. The invention further develops a dispersible oil suspending agent, and the suspending rate is effectively improved through formula optimization. The dosage form takes soybean oil as a dispersion medium, does not contain harmful solvents such as methylbenzene, is free of dust pollution and is friendly to operators and the environment, and ascorbic acid is added to improve the ultraviolet resistance of spores and prolong the effective period of the pesticide effect. In addition, the preparation also has a certain control effect on caffeinidae, is wide in application scene, provides a new scheme for green control of spodoptera frugiperda, solves the problems of chemical pesticide resistance and residue, and accords with the development requirements of green agriculture.

The invention discloses a fertilizer containing herbaceous plant components and a preparation method thereof, and particularly relates to the technical field of agricultural waste, the formula is composed of a composite traditional Chinese medicine extract, a core-shell structure carrier, a functional probiotic agent and an auxiliary agent, and the preparation method comprises the steps of S1, graded extraction of traditional Chinese medicines, S2, carrier loading and microcapsule preparation, and S3, probiotic integration and granulation. Through scientific compatibility of the compound traditional Chinese medicine extracts, design of the core-shell structure carrier, directional addition of the functional probiotics and an optimized preparation process, the prepared fertilizer can synergistically improve the growth index and quality of crops, remarkably enhance the disease and pest resistance, effectively improve the physicochemical properties and micro-ecological environment of soil and improve the yield of the crops. The usage amount of chemical pesticides and fertilizers is reduced, integration of nutrition supply, disease prevention and control and soil improvement is achieved, and the requirements of green agriculture development are met.

The invention provides an ecological restoration method for backfilling dehydrated slime into a bauxite goaf, which comprises the following steps of: backfilling filtered and dehydrated slime into the goaf, and covering soil; and then land leveling, slope treatment, soil improvement, irrigation and drainage engineering, field road construction, farmland protection, ecological environment protection, science and technology supporting service and other processes are carried out. Organic fertilizers (such as pig manure and chicken manure) and a biological silicon fertilizer are added on the basis of an inorganic fertilizer, so that the physical and chemical properties of soil are more effectively improved, and meanwhile, the risk of heavy metals in the soil is reduced; by implementing a terraced field slope descending measure in the goaf, the land is smoother, water and soil loss is effectively prevented, the soil fertility is kept, the repaired land can be returned to farmers for planting common crops such as corn, peanuts and cassava, the income of the farmers can be increased, and local agricultural development can be promoted.

This application provides a land assessment method, system, electronic device, and computer-readable storage medium, relating to the field of land assessment technology. The land assessment method includes: acquiring multiple candidate evaluation indicators for a target area, and actual changes in saline-alkali land in the target area over historical periods; based on correlation analysis between the candidate evaluation indicators and the actual changes in saline-alkali land, selecting key evaluation indicators from the candidate indicators, determining the weights of the key evaluation indicators, and the membership functions of each key evaluation indicator; based on the weights and membership functions, calculating the suitability index of each assessment unit within the target area, and classifying suitability levels according to the suitability index to obtain the suitability assessment results. This results in a continuous index and level classification map that reflects the relative suitability of different geographic spatial units for agricultural development, providing an intuitive and comparable decision-making basis for the differentiated and refined development of land resources.

This invention relates to the fields of pesticide formulations and pesticide pest control, specifically to a composite nano-slow-release suspension of emamectin benzoate and chlorantraniliprole, its preparation, and application. The nano-slow-release suspension comprises emamectin benzoate, chlorantraniliprole, a nanocarrier, a wetting agent, a dispersant, a synergist, a thickener, an antifoaming agent, an antifreeze, a preservative, and water. The nanocarrier is an NX-CMCS & MOF composite nanocarrier. Spraying this composite nano-pesticide onto crops improves the adhesion and penetration of the pesticide on leaves, enhances its resistance to rain washout, and effectively kills major crop pests such as bollworms, diamondback moths, cowpea thrips, and rice planthoppers. Compared with traditional pesticide formulations, it has a significant effect of reducing dosage and increasing efficacy, and has broad application prospects in the field of agricultural pest control and promoting green agriculture.

The invention provides a strain of underground neosphingobacterium AR5-1 and application thereof in growth promotion and drought resistance, and the strain is named as Novosphingobacterium subraneum in taxonomy, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.35273. The invention also provides a method for preparing the underground neosphingobacterium AR5-1. The invention further provides an application of the underground neosphingobacterium AR5-1 in growth promotion and drought resistance. The underground neosphingosine bacterium is separated from tobacco rhizosphere drought stress soil, and through molecular identification (the similarity of 16S rRNA is 100%) and functional verification, the underground neosphingosine bacterium has the capacity of producing indoleacetic acid (the concentration of IAA is 0.142 + / -0.005 mg / mL) and fixing nitrogen. A bacterial suspension (OD600 = 0.3) prepared from the bacillus subtilis is applied to plant rhizosphere, so that the growth of crops such as tobacco and the like can be remarkably promoted (the biomass is increased by more than or equal to 20%), the drought resistance is enhanced (the drought rehydration recovery rate is increased by 35%), and the bacillus subtilis has the advantages of environmental friendliness and reduction of chemical fertilizer dependence, and is suitable for sustainable agricultural development.

The application discloses a Vibrio parahaemolyticus quorum sensing inhibiting peptide and application thereof, and belongs to the field of bioactive peptides. The inhibiting peptide comprises the following amino acid sequence: Ser-Phe; the application further discloses application of the quorum sensing inhibiting peptide in inhibiting Vibrio parahaemolyticus or preparing antibacterial drugs for inhibiting Vibrio parahaemolyticus. The application adopts a molecular docking technology to screen an active peptide having an inhibiting effect on a Vibrio parahaemolyticus LuxS / AI-2 quorum sensing system, ADMET property prediction is carried out on a peptide segment with better binding force, and finally, a bioactive peptide SF stably combined with LuxP, LuxQ and LuxS is obtained; the dipeptide has good in-vitro inhibiting activity on the Vibrio parahaemolyticus LuxS / AI-2 quorum sensing, and has no cytotoxicity. The dipeptide obtained by the application can be used for guiding development of a new type of antibacterial preparation applied to food, medicine and agriculture and other fields, and has great significance for human health and agricultural development.

The invention relates to the technical field of straw degrading microbial inoculants, in particular to a bacterium-algae composite microbial inoculant capable of efficiently degrading straw and reducing emission and sequestering carbon as well as a preparation method and application of the bacterium-algae composite microbial inoculant, and the bacterium-algae composite microbial inoculant is prepared by mixing blue-green algae and Cellulomonas illani according to a mass ratio of 1: (3-9), preferably 1: 7. The preparation method comprises the following steps: respectively culturing blue-green algae and strains to specific optical densities, centrifugally collecting algae and thalli, mixing according to a ratio, complementing the mass with humic acid, and drying and powdering to obtain the microbial inoculum. When the complex microbial inoculant is applied according to 10 g / m < 2 > after straw returning to the field, the straw degradation efficiency can be remarkably improved, meanwhile, through the carbon sequestration effect of blue-green algae, emission of CO2 and CH4 is reduced, straw carbon is promoted to be converted into soil organic carbon, the soil carbon sequestration function is enhanced, the crop growth performance is improved, the synergistic interaction of straw degradation, carbon emission reduction and soil recarburization is achieved, and the crop yield is improved. The method is suitable for agricultural waste recycling and green low-carbon agricultural development.

The utility model discloses an air-blowing type wheat and rice precision seeder which comprises a fertilizer box assembly, a seed box assembly, a rack assembly, a rotary cultivator, a pressing roller, a fertilizing disc, a lower frame assembly and a seeding single body, the fertilizer box assembly and the seed box assembly are both fixedly arranged on the front upper portion of the rack assembly, the rotary cultivator is arranged below the rack assembly, the pressing roller is connected with the rotary cultivator, and the fertilizing disc is connected with the lower frame assembly. The lower frame assembly is connected with the pressing roller, and the fertilizing disc and the seeding single body are sequentially arranged on the lower frame assembly and located below the rear portion of the seed box assembly. According to the utility model, various functions of rotary tillage, ditching, pressing, fertilization and seeding can be integrated through mutual cooperation of all the components, so that the seeding efficiency is improved, the cost is reduced, the seeding quality is ensured, the modern agricultural production requirements are met, the intelligent operation of the seeding machine is realized, and the agricultural development is facilitated.

This invention relates to a bioactivator based on L-proline structural modification, its synthesis method, and its application, belonging to the field of agricultural chemistry. The activator employs a biomimetic molecular design strategy, prepared through the splicing of active structural units. L-proline's unique natural source advantage and the important active unit fragment N,N-diethylethanolamine from DA-6 undergo an esterification reaction to yield 2-(diethylamino)ethyl-L-proline ester. Bioactivity experiments were conducted on wheat and soybean, respectively. The results showed that this compound can promote plant growth at low concentrations, exhibiting significantly better growth effects than the blank control in terms of germination rate, root length, and stem length. Furthermore, the preparation process of this invention is simple, low-cost, and easily scalable for industrial production, and it possesses good environmental compatibility. Its unique natural source advantage provides a new option for activator development and has significant potential for agricultural development.

The application belongs to the technical field of microbial application, and specifically discloses application of roseofungin Streptomyces roseofunginus in plant disease prevention and treatment. The application finds that after R&M synthesis genes in roseofungin Streptomyces roseofunginus are knocked out, the strain can still synthesize oxazolomycin A. Further finding is that oxazolomycin A shows good inhibitory effect on pepper Phytophthora capsici and Phytophthora sansomei, wherein the EC 50 value of pepper Phytophthora capsici PC35 is 2.95 μg / mL, and the EC 50 value of atractylodes Phytophthora sansomei AMPH-1 is 2.39 μg / mL. The oxazolomycin A prepared by the application can be developed into a biological pesticide that can effectively meet the agricultural development demand of "green ecology", and provides key biocontrol resource support for green prevention and control of plant diseases.

The invention belongs to the technical field of wheat breeding, and particularly relates to a wheat breeding method capable of resisting wheat stem bee pests. Aiming at occurrence and damage of wheat insect pests, the invention provides a breeding method for highly resisting wheat stem bee pests, according to the cultivated wheat strain material, the stem tube wall under the spike is obviously thickened, so that the ovulation device of female adult wheat stem bees cannot puncture the stem tube wall under the spike, and eggs cannot be injected into the wheat stem, thereby effectively achieving the insect-resistant effect; even if individual female bees successfully inject eggs, stem pith injection is slow after larvae are hatched by the eggs, before wheat is mature, stem bases cannot be injected with the eggs, broken stem loops cannot be formed, wheat stems cannot lodging, and the larvae cannot make cocoons, pupate and overwinter at the roots, so that reproduction and offspring of the wheat stem bees are blocked. The method can effectively prevent the damage of the wheat stem bees, solves the problem of prevention and control of the damage of the wheat stem bees for wheat production, effectively reduces the dependence on pesticides, and has important guiding significance for sustainable development of agriculture.

The invention discloses a nitrogen fertilizer application method for promoting straw decomposition and efficient utilization of nitrogen, which comprises the following steps: determining the total nitrogen fertilizer application amount by using basic soil fertility yield, target yield and nitrogen fertilizer agronomic utilization efficiency indexes, returning straws to the field, burying the straws into a soil layer on the day, and determining the nitrogen application amount in different growth periods according to the straw decomposition rate of plants in different growth periods. The method does not need to measure the nitrogen content of soil / plants, reduces the material and labor cost, and is high in timeliness; the problems of nitrogen competition in the early stage, excessive nitrogen supply in the middle and later stages, high conventional technical requirements, long regulation and control period and the like after straw returning to the field are solved; release of straw nutrients (nitrogen, phosphorus, potassium and trace elements) is promoted, the soil organic matter and rice yield is increased, weight losing and efficiency increasing are assisted, and green agriculture development is achieved; the method is suitable for indica rice, japonica rice and hybrid rice varieties, and is convenient for large-area popularization.

The invention discloses a special forest-fruit water-fertilizer collaborative precise irrigation method using tunnel gushing wastewater, relates to the field of water-fertilizer collaborative irrigation, and aims to construct a tunnel gushing wastewater water quality dynamic database, establish a mapping relationship between special forest-fruit physiological signals and water-fertilizer demands, and integrate a drought valley microclimate mutation response mechanism. And calculating a single irrigation amount and an exogenous fertilizer amount based on a three-dimensional coupling water and fertilizer proportioning coefficient algorithm, and generating a dynamic water and fertilizer irrigation execution scheme. According to the method, the resource utilization of the tunnel gushing wastewater is realized, the gushing water subjected to pretreatment and deep purification is used for irrigation of characteristic forests and fruits, the problem of shortage of irrigation water in arid river valleys is effectively solved, and the waste of water resources is reduced. Part of exogenous fertilizer is replaced with gushing water natural mineral substances, chemical fertilizer input is reduced, planting cost is reduced, potential pollution of chemical fertilizer to soil and surrounding environment is reduced, and the requirement for green agriculture development is met.

According to the folium artemisiae argyi extract, the preparation method thereof and the application of the folium artemisiae argyi extract in growth promotion, the folium artemisiae argyi extract required by a plant biostimulant comes from folium artemisiae argyi, the folium artemisiae argyi extract has the advantages of being rich in source, easy to extract and the like, green and sustainable agricultural development is facilitated, and a new scheme is provided for yield increase of crops. According to the extract, leaves and stems of traditional Chinese medicine folium artemisiae argyi serve as raw materials and are subjected to two times of ultrasonic extraction (absolute ethyl alcohol), filtrate is combined, then a crude extract is prepared, a purified component is a 60% ethanol component obtained by separating the crude extract through macroporous resin, the purified component mainly comprises a flavonoid component, and the content of eupatilin is the highest. The plant height, leaf length and fresh weight of rice, pakchoi, cotton and soybean are all improved after the fertilizer is applied according to the application mode mentioned in the invention.

This invention discloses a deep-sea Bacillus strain and its applications, belonging to the field of agricultural microbiology and biocontrol technology. This invention screened and identified a deep-sea Bacillus strain NT-3-7. Experimental verification showed that the deep-sea Bacillus strain NT-3-7 provided by this invention is tolerant of saline-alkali environments (it can grow in 15% NaCl and pH 9.0 environments) and possesses good nitrogen-fixing, phosphorus-solubilizing, and potassium-solubilizing abilities; it has excellent growth-promoting effects on plants, increasing plant height, taproot length, root dry weight, and stem and leaf weight of chili pepper plants. Using the deep-sea Bacillus strain NT-3-7 provided by this invention to prepare products that promote plant growth can help promote sustainable agricultural development while significantly reducing environmental pollution, showing promising application prospects.

This invention discloses the application of the OsSR1IP1 gene in regulating tillering angle in rice. It belongs to the field of plant genetic engineering technology. This invention uses genetic engineering technology to obtain transgenic rice that stably overexpresses OsSR1IP1. By comparing the growth of wild-type and OsSR1IP1-overexpressing transgenic rice, it was found that the tillering angle of OsSR1IP1-overexpressing transgenic rice plants was significantly increased. Therefore, this invention successfully identified a key gene regulating rice plant architecture, which has theoretical significance and application value for enhancing the lodging resistance and increasing yield of rice varieties, and is of great importance to agricultural development and national food security.

The invention relates to the technical field of green prevention and control of agricultural pests, in particular to preparation and application of an insecticidal fungus compound for efficiently preventing and controlling spodoptera frugiperda. Diatomite is dispersed in a dispersing agent, and diatomite dispersion liquid is obtained; the preparation method comprises the following steps: dispersing chitosan in a dispersing agent to obtain a chitosan dispersion liquid; dispersing the insecticidal fungal spores in a dispersant to obtain an insecticidal fungal spore dispersion liquid; and mixing the diatomite dispersion liquid, the chitosan dispersion liquid and the insecticidal fungal spore dispersion liquid to obtain the insecticidal fungal compound. The insecticidal fungus compound has a good control effect on major agricultural pests such as spodoptera frugiperda and has a wide application prospect in the field of agricultural pest control, promotion of green agriculture development and the like.

The invention relates to the technical field of green prevention and control of plant diseases, in particular to application of a cucumber-marigold intercropping mode to prevention and control of cucumber root knot nematode diseases. Cucumbers and marigold are intercropped and cultivated, and cucumber root-knot nematode infection and / or reproduction are / is inhibited by the allelopathy of the marigold; wherein marigold plants are planted in soil and / or a culture medium, so that stable root systems are formed before cucumber planting; 7-21 days after field planting of the marigold, cucumber seedlings are planted in the same cultivation container or the adjacent rhizosphere space, the root system of the cucumber and the root system of the marigold are located in the same cultivation environment but not directly twined, and the marigold keeps surviving and growing in the early stage and the middle stage of cucumber growth. The method solves the problem that an existing intercropping mode lacks a system method with a cultivation structure and a planting time sequence as the core, and has the advantages that operation is easy and convenient, applicability is high, popularization is easy, compared with cucumber single cropping, the root knot index of cucumbers under an intercropping system is remarkably reduced, and the requirement for green agriculture development is met.

The invention discloses a preparation method and application of a radix angelicae pubescentis extract, and particularly relates to the technical field of agricultural biology. The preparation method comprises the following steps: crushing radix angelicae pubescentis, sieving with a 40-100-mesh sieve, extracting with absolute ethyl alcohol, and concentrating under reduced pressure to obtain a pasty extract; in the aspect of application, the seed soaking agent is applied to rice, wheat or corn planting, for example, seed soaking treatment is performed on rice, wheat or corn, and the concentration is 10-5000 mg / L, preferably 500 mg / L. The extract is natural, safe and environment-friendly, and conforms to the development strategy of green agriculture. The fertilizer can promote rapid growth of rice, wheat or corn in the seedling stage, help to establish ecological competitive advantages, and relieve weed prevention and control pressure; and the resistance of rice, wheat or corn to adversities such as drought, salt stress and the like can be enhanced. Compared with a traditional growth promoting material and a compound preparation, the preparation process is simple and convenient, the cost is low, more farmers can accept the growth promoting material, breakthrough progress is brought to the rice, wheat or corn planting industry, and the development of a planting area is promoted.

The utility model relates to the technical field of agricultural irrigation equipment, in particular to buffering type water outlet equipment which comprises a box body, a first opening is formed in the box body, a flow guide cylinder is arranged in a water containing cavity in the box body, a stud is rotatably installed in an inner cavity of the flow guide cylinder, and a water baffle is fixedly sleeved on the outer wall of the stud. The peripheral wall of the water baffle is in sliding fit with the inner wall of the guide cylinder, the side wall of the guide cylinder is connected with a water outlet cylinder which is horizontally arranged, and an inner cavity of the guide cylinder is communicated with an inner cavity of the water outlet cylinder; a water outlet is formed in the side wall of the box body, and the arrangement height of the water outlet is the same as that of the water outlet barrel. The flow guide cylinder can bear pressure fluctuation when a water pump or a valve is started or stopped, damage to the water outlet equipment structure is avoided, meanwhile, water discharged from the water outlet plug can slowly flow into farmland through the water containing cavity, direct impact on the soil surface is avoided, the surface soil loss rate is reduced, the soil fertility is protected, the crop yield is further ensured, and agricultural development is guaranteed.

This invention belongs to the field of harvesting technology and discloses a multifunctional green Sichuan pepper harvesting machine and method based on stakes. The multifunctional green Sichuan pepper harvesting machine includes a fruit-picking device, a sieving device installed on one side of the fruit-picking device, and a crushing device installed on the other side of the sieving device. This invention enables the grasping, fixing, and harvesting of green Sichuan peppers through the fruit-picking device, separates the green Sichuan pepper branches and green Sichuan pepper granules through the sieving device, and then crushes the separated green Sichuan pepper branches and impurities through the crushing device. This allows for large-scale harvesting of green Sichuan pepper granules, facilitating subsequent disposal or reuse. The smooth connection between the various parts of the device makes green Sichuan pepper harvesting simpler and more convenient, thus contributing to agricultural development.

The utility model relates to the technical field of pig manure deep processing, in particular to a deep processing device using pig manure as alternative fuel, which comprises a pig manure solid-liquid separation mechanism, the pig manure solid-liquid separation mechanism is connected with a pig manure stacking mechanism through a conveying device, the pig manure stacking mechanism is connected with a pig manure crushing mechanism through a conveying device, and the pig manure crushing mechanism is connected with a pig manure conveying device. And the pig manure crushing mechanism is connected with a pig manure fuel distribution mechanism through feeding equipment. Waste pig manure of a live pig breeding enterprise is utilized to generate heat value for use, the value of the pig manure is created, environmental pollution is reduced, water obtained after separation of the waste pig manure is filtered and reused, water resources are saved, cement enterprises utilize the pig manure subjected to filter pressing for calcination, coal consumption is reduced, energy is saved, environmental pollution is reduced, and the economic benefit is increased. And moreover, conditions are created for deep cooperation of industrial and agricultural development, more values are created for agricultural development and farmer income creation, an optimal cooperation point is found for comprehensive utilization of wastes, and mutual complementation is realized.

The invention discloses a novel vegetable health magnesium resistance inducer as well as a preparation method and application thereof, the main components of the vegetable health magnesium resistance inducer are trimethylglycine (GB) and magnesium oxide nanoparticles (MgONPs), and the components have the characteristics of no toxicity, harmlessness, no pollution and the like. The invention finds that the'vegetable health magnesium 'resistance inducer can activate plant hormone signals, enhance plant immunity and resist virus diseases. The composition can be sprayed to vegetable crops before the high-incidence period of vegetable virus diseases to prevent and treat virus diseases, is simple and convenient, has strong operability, and can effectively improve the broad-spectrum resistance of plants to various vegetable virus diseases. Compared with traditional pesticides, the composition is more green and safer, meets the requirements of sustainable and healthy development of agriculture, and has huge application potential.

The application discloses a bacillus licheniformis-derived nematocidal peptide Peptide-1 and application of the nematocidal peptide Peptide-1 in the prevention and control of root-knot nematode diseases, wherein the amino acid sequence of the nematocidal peptide Peptide-1 is shown as SEQ ID NO. 1. The application further provides a medicament for preventing and controlling root-knot nematodes and a prevention and control method thereof. The activity verification result shows that under the condition of 1.00 mmol / L, the corrected mortality of the nematocidal peptide Peptide-1 to the second instar larvae of root-knot nematodes reaches 90.7% after 24 hours, and the nematocidal peptide Peptide-1 has a clear and efficient lethal effect and concentration dependence. The nematocidal peptide Peptide-1 can be used for the green prevention and control of root-knot nematodes and the creation of new germplasm resources of root-knot nematode-resistant crops, meets the requirements of green agricultural development, and can effectively solve the problems of pesticide residues, drug resistance and ecological pollution caused by traditional chemical nematocidal agents.

The invention discloses an FvePREs transcription factor and application thereof in promoting curling of strawberry petals, in a general agricultural cultivation technology, the petals are curled by gibberellin (GA3) or a growth regulator for actively inducing the curling of the petals, and excessive hormone may cause malformation and curling of the petals and also may cause side effects such as fruit hollowness, quality reduction and the like. According to the scheme, the gene technology is ingeniously utilized to directly act on a plant endogenous regulation and control system, the synergistic effect of strawberry petal curling and quality optimization is achieved by activating or inhibiting specific gene expression (such as cell division and nutrient distribution), and in addition, the over-expression FvePREs transcription factor can be used for improving the yield of strawberry petals by changing the hereditary characteristics of strawberries. The good petal curling effect can be kept in the long-term planting process, dependence on external chemical substances can be reduced, and sustainable agricultural development is facilitated.

The application discloses an application of Medicago sativa MsCEPR1 gene and protein coded by the same in low-phosphorus stress tolerance of Medicago sativa and belongs to the technical field of plant genetic engineering. The Medicago sativa MsCEPR1 gene provided in the application has a nucleotide sequence as shown in SEQ ID NO. 1. The application preliminarily reveals the regulation of CEPR1 on phosphorus absorption related genes by exploring the function of CEPR1 in phosphorus absorption, provides a basis for constructing a molecular network of CEPR1 regulating phosphorus absorption, and provides a direction for cultivating Medicago sativa varieties with high phosphorus absorption, which has important significance for reducing the dependence on phosphorus fertilizer and sustainable agricultural development.