129 results about "Trichoderma sp." patented technology

The invention provides a control method for apple alternaria leaf spot, and belongs to the technical field of crop disease control. The prevention and treatment method comprises the following steps: spraying a foliar biological pesticide fertilizer to apple trees; the foliar biological medical fertilizer comprises a bacillus fermentation product and a trichoderma fermentation product, the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208, and the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208; the trichoderma is at least one of T6 and Ym. The combined action of bacillus and trichoderma is utilized, the morbidity of apple alternaria leaf spot can be effectively reduced, the growth of apple trees can be promoted, the photosynthetic capacity is improved, the combination of disease prevention and yield increase is realized, and the method is environment-friendly and suitable for green agricultural development.

The invention discloses a microbial agent and application thereof in agricultural production. The microbial agent is prepared by compounding seed solutions of two or more of seed solutions of Trichoderma harzianum, Trichoderma brevicompactum Tb12, Gliocladium roseum NF-06 and Trichoderma asperellum, and the seed solutions of the two or more of the Trichoderma harzianum, the Trichoderma brevicompactum Tb12, the Gliocladium roseum NF-06, the Trichoderma asperellum, the Trichoderma harzianum, the Trichoderma brevicompactum, the Gliocladium roseum NF-06 and the Trichoderma asperellum. The selected strain combination can effectively prevent and treat fungal diseases and nematode diseases and can promote plant growth. The trichoderma harzianum and the trichoderma brevicompactum Tb12 are used in a combined mode, the control effect on fusarium is obvious, the gliocladium roseum NF-06 and the trichoderma asperellum are used in a combined mode, the nematode killing effect is obvious, and the growth of rice is promoted through the trichoderma harzianum and the trichoderma asperellum.

The invention belongs to the technical field of agricultural microbial control, and particularly relates to a growth-promoting and disease-preventing compound microbial agent and a preparation method and application thereof, and the compound microbial agent specifically comprises microbial powder, a growth auxiliary material and a drying agent; the microorganisms comprise vermiform yellow, trichoderma harzianum, bacillus velezensis, bacillus licheniformis and brevibacillus laterosporus. According to the compound microbial agent, through the synergistic effect of multiple strains, the control effect of the compound microbial agent on verticillium dahliae is enhanced, and the defects that a single biocontrol bacterium is single in biocontrol mechanism, poor in environment adaptability, incapable of being colonized in soil for a long time and unstable in control effect are overcome; the compound microbial agent can increase the yield of cotton and pepper and improve the quality of cotton fibers, and provides scientific and technological support and decision service for realizing green, environment-friendly, cost-saving and efficiency-increasing modern agriculture.

The present invention relates to a composition comprising components (A) and (B) as active ingredients, wherein component (A) comprises a Streptomyces and component (B) is a compound selected from the group consisting of sulphur, phosphonate, copper hydroxide, copper oxychloride, bicarbonate, Bacillus sp, Trichoderma sp, aureobasidin A, jawsamycin, kasugamycin, natamycin, a polysaccharide elicitor, a plant biostimulant (PBS) comprising an algal extract, a plant extract and / or vinasse, orange oil, and / or tea tree oil. The invention further relates to a method of controlling or preventing phytopathogenic diseases, preferably phytopathogenic fungi, on a plant or on plant propagation material and / or on harvested food crops, which comprises applying to the plant, on plant propagation material, the locus thereof, and / or on harvested food crops the composition according to the present invention.

The invention discloses a trichoderma virens preparation capable of preventing diseases and increasing yield as well as a preparation method and application of the trichoderma virens preparation. Belongs to the technical field of biological agents. According to the trichoderma virens strain T259 disclosed by the invention, the trichoderma virens strain T259 is respectively subjected to strain amplification culture, liquid amplification culture and solid fermentation culture, a solid fermentation product of the T259 strain is successfully prepared, the solid fermentation product is mixed with an auxiliary agent, and the trichoderma virens preparation capable of preventing diseases and increasing yield is prepared. The trichoderma virens spore content in the preparation is improved; solid fermentation can effectively improve the colonization ability of trichoderma virens in the field. The production of the trichoderma virens preparation has the characteristics of large sporulation quantity, short period, wide raw material source and low cost; the fertilizer is easy to use and operate, suitable for various crops and remarkable in disease prevention and yield increase effect. And a foundation is laid for quality and efficiency improvement and high-quality and high-efficiency development of modern agriculture.

The invention relates to the technical field of soil improvement, and particularly discloses a soil conditioner based on microbial community regulation and control and a preparation method thereof, the soil conditioner comprises: (a) a composite functional flora comprising nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium-dissolving actinomycetes and trichoderma, and the viable count ratio of the nitrogen-fixing bacteria to the phosphate solubilizing bacteria to the potassium-dissolving actinomycetes to the trichoderma is (1.0-1.5): (0.8-1.2): (0.5-1.0): (0.3-0.8); b) modified biochar carrier: the modified biochar carrier is prepared by oxygen-limited thermal cracking of agricultural wastes and is subjected to surface modification by a chitosan solution with a mass fraction of 3-5%; (c) a synergistic component which comprises humic acid particles and an enzymolysis seaweed extract; (d) a flora activator: compounding fructo-oligosaccharide and L-aspartic acid according to a mass ratio of 2: 1; through the synergistic effect of multifunctional composite flora of nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium-solubilizing actinomycetes and trichoderma, the biotransformation process of nitrogen, phosphorus and potassium elements in soil is synchronously activated, and the modified charcoal carrier is subjected to multi-stage pyrolysis and chitosan modification to form a graded pore structure.

The invention relates to the technical field of agricultural microorganisms, and discloses a complex microbial inoculant for improving soil micro-ecology and promoting plant growth and application thereof.The complex microbial inoculant is prepared from 30-40 parts of bacillus subtilis, 20-40 parts of pseudomonas, 10-20 parts of nitrogen-fixing bacteria, 5-10 parts of photosynthetic bacteria, 5-10 parts of trichoderma and 5-10 parts of penicillium; wherein the bacillus subtilis is bacillus subtilis L11 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.31274, the preservation date is July 11, 2024, and the bacillus subtilis is classified and named as bacillus subtilis. The complex microbial inoculant for improving the soil micro-ecology and promoting the plant growth is adopted, a multi-bacterium synergistic community is constructed, the complementary metabolism advantage of different strains at the rhizosphere is utilized, the nutrient conversion and stress resistance characteristics are improved, and therefore the soil micro-ecology is improved, and the plant root development is promoted.

The invention provides a trichoderma fungicide for promoting growth of allium plants and application of the trichoderma fungicide, the trichoderma fungicide is a trichoderma harzianum strain MCBC3, the trichoderma fungicide and diammonium phosphate are applied in a combined mode, the application amount of the diammonium phosphate is 15 kg / mu, and the application amount of the trichoderma fungicide is 1 L / mu; existing trichoderma resource development focuses on a normal soil environment, the trichoderma harzianum MCBC3 is screened out from the saline-alkali soil for the first time, the salt resistance and the alkali resistance of the trichoderma harzianum MCBC3 directly aim at double stress of high salt and high alkali of the saline-alkali soil, and the technical bottlenecks that a traditional strain is poor in adaptability and low in survival rate in an extreme environment are solved.

The invention discloses a trichoderma complex microbial inoculant capable of promoting wheat growth and preventing and treating wheat stem rot and application of the trichoderma complex microbial inoculant. The trichoderma complex microbial inoculant comprises a trichoderma asperellum strain XX-57 and a trichoderma harzianum strain PY-127, and the preservation number of the trichoderma asperellum strain XX-57 is CGMCC No. 41937; the preservation number of the trichoderma harzianum strain PY-127 is CGMCC (China General Microbiological Culture Collection Center) NO. 41938. The invention further specifically discloses application of the trichoderma complex microbial inoculant in promoting wheat seed germination and wheat growth and preventing and treating wheat basal stem rot, the trichoderma complex microbial inoculant is applied 10-20 days after wheat seedling emergence, and it is found that compared with single trichoderma inoculation, the trichoderma complex microbial inoculant has the promoting effect on wheat plant height, root length, SPAD value, fresh weight and dry weight; the control effect on wheat stem rot is better, and particularly the control effect is more obvious when pathogenic bacteria are inoculated in advance.

The application discloses application of Trichoderma asperellum strain YN4 and fungicides in prevention and treatment of plant fungal diseases. The application research shows that the Trichoderma asperellum strain YN4 has a good inhibitory effect on pepper anthracnose, rubber anthracnose, mango anthracnose, soybean anthracnose and the like. Further, compared with the single use of fungicides or the Trichoderma asperellum strain, the inhibitory effect of the combination of the two is better. Under certain conditions, the Trichoderma asperellum strain is combined with 10% difenconazole water dispersible granules, 70% thiophanate-methyl wettable powder or 450g / L prochloraz emulsion in water, and has a synergistic effect. Not only can the use amount of fungicides be greatly reduced, but also the inhibitory effect on plant pathogenic fungi can be further improved, the incidence can be reduced, the inhibition on pathogenic bacteria can be enhanced, the use and residue of chemical pesticides can be reduced, and technical support is provided for the selection of fungicides for pepper anthracnose and the like and the combination of bacteria and drugs.

This invention relates to a composition comprising an active ingredient A and an active ingredient B, wherein the active ingredient A comprises a phosphorus-based compound, and the active ingredient B comprises a compound selected from Trichoderma sp.

The invention discloses an application of trichoderma griseaginii TR2122 in prevention and treatment of beet root rot, a strain of the trichoderma griseaginii TR2122 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.21025, and the strain has strong antagonism and hyperparasitism effects on fusarium oxysporum causing the beet root rot. Meanwhile, the invention further provides a microbial preparation containing the live spores of the strain, the spore content is not lower than 2 * 10 < 8 > CFU / g, and the microbial preparation is preferably wettable powder containing a parent drug, a dispersing agent, a wetting agent and filler. In addition, the invention further discloses a preparation method of the preparation, and the preparation method comprises the steps of fermentation culture, concentration drying and mixing preparation. The strain and the preparation have stable and lasting control effect on beet root rot, are superior to or equivalent to conventional chemical agents, are safe and environment-friendly, and provide an effective solution for beet green control.

The invention discloses a preparation method and a use method of a sorghum biological seed-dressing agent, the biological seed-dressing agent comprises a seed-dressing agent 1 and a seed-dressing agent 2, the preparation method comprises the following steps: (1) mixing bacillus subtilis, trichoderma and corn flour, adding water, stirring into paste, and culturing at 25-30 DEG C for 48-60 hours to obtain thalli; (2) kneading the thalli into cubes, drying at room temperature, and grinding to obtain a seed dressing agent 1; and (3) uniformly mixing sodium alginate with water to obtain a seed dressing agent 2. The biological seed dressing agent can significantly improve the emergence rate of sorghum seeds, promote the growth and development of seedlings, and enhance the smut resistance of sorghum plants.

The invention relates to the technical field of orchard planting and soil fertilization management, and discloses a mango orchard ecological fertilization planting method with root zone annular drip covering and inter-row pasture mixed sowing, which comprises the following steps: field foundation pretreatment: field shallow rotation no-tillage, ecological ditch digging and biological herbicide spraying; paving a domain composite covering layer constructed by root domain composite covering and intelligent drip irrigation, clamping a drip irrigation ring, and controlling drip irrigation by a sensor; mixed sowing of the ternary pasture between the rows in the rainy season is conducted, and the ternary pasture is cut and utilized in good time; after composting the mushroom dregs, the mushroom dregs of the double-microbial-agent synergistic compost, the straws and the trimmed mango tree branches and leaves, adding double microbial agents of bacillus subtilis powder and trichoderma powder, and precisely fertilizing; and precise fertilization, ecological disease prevention and control and field maintenance: irrigating roots with trichoderma in a flowering period, and maintaining drip irrigation and a covering layer. According to the mango garden ecological fertilizing planting method with root domain annular dripping covering and inter-row pasture mixed sowing, the soil fertility can be improved, diseases can be prevented, and the quality and yield of mangoes can be improved.

The present application relates to a kind of bacteria inhibiting and growth promoting microorganism and its compound microbial inoculant, preparation method and application.The present application provides a kind of bacillus subtilis (Bacillus subtilis) YCBA0319, preservation number is CGMCC No.33006;A kind of trichoderma longibrachiatum (Trichoderma longibrachiatum) YCFA0522, preservation number is CGMCC No.41655.In the technical solution of the present application, through bacillus subtilis and trichoderma longibrachiatum and the compound microbial inoculant of its composition, through the synergistic effect of two strains, it has the promotion effect to soil health and apple yield, quality etc., compared with control, soil bulk density is reduced, organic matter and available nutrient are improved, and apple single fruit size, yield and quality are all improved.

The invention discloses a Chinese angelica out-of-season single seedling raising method suitable for mechanical transplanting and application thereof, and belongs to the field of Chinese herbal medicine seedling raising and cultivation. According to the method, a sunlight greenhouse or a gutter-connected greenhouse with the altitude being 2300 m or above and the lowest temperature being larger than or equal to 8 DEG C in winter is selected, field soil without continuous cropping obstacles is matched with fried rapeseeds, trichoderma harzianum and bacillus subtilis to prepare a special matrix, a seedling raising paper book with the single-hole diameter being 19-25 mm and the height being 12 cm is adopted, pelletized seeds are dibbled in a double-grain mode from late November to early December, and the seeds are sown in the seedling raising paper book with the single-hole diameter being 19-25 mm and the height being 12 cm. Through management of precise temperature and humidity regulation and control, foliage dressing, seedling hardening and leaf cutting and the like, standardized single seedlings are cultivated and transplanted through a transplanter. According to the method, the early bolting rate of the angelica sinensis is reduced, the seedling uniformity and the transplanting survival rate are improved, the transplanting efficiency is improved, the labor cost is reduced, the yield of the angelica sinensis can be increased, efficient utilization of out-of-season resources is achieved, and support is provided for industrial large-scale standardized development.

The invention discloses a method for preventing and treating sclerotinia rot of colza by antagonistic trichoderma strains, and relates to the technical field of agricultural disease prevention and control, and the method comprises the following specific steps: S100, strain screening and mutagenesis: separating trichoderma strains from rhizosphere soil of colza in different environments, primarily screening strains with antagonistic action on sclerotinia sclerotiorum by a plate confrontation method, and carrying out mutagenesis on the strains with antagonistic action on sclerotinia sclerotiorum; carrying out chemical mutagenesis treatment on the primarily screened strains, carrying out confrontation culture on the primarily screened strains and sclerotinia sclerotiorum again, screening out high-yield antagonistic substances with larger inhibition zones and mutant strains with strong stress resistance, and culturing to obtain trichoderma fermentation liquor; by obtaining the trichoderma strains which can keep efficient antagonism and strong stress resistance under different environmental conditions, the stability of the trichoderma strains on disease control is enhanced, so that the rape can effectively resist invasion of sclerotiniose under different environmental conditions in the growth process, and the disease resistance of the rape is improved. And antagonistic active substances are extracted to prepare a biological fertilizer carrier, and the biological fertilizer carrier is applied to the planting process of oilseed rape, so that the growth and reproduction of sclerotinia sclerotiorum are effectively inhibited.

The application discloses a kind of low temperature resistant strain YW-1 for preventing and treating rice seedling wilt, and belongs to the field of plant protection.The low temperature resistant strain YW-1 is a pseudo-odor class fungus, and the application proves that the strain YW-1 can maintain normal growth and metabolism in a low temperature environment of 15 DEG C, and can effectively destroy the hyphal structure of the wilt pathogen by secreting cellulase and chitinase, thereby inhibiting the occurrence of the disease from the source.The strain YW-1 has no antagonism with the commonly used functional fungus Trichoderma in the seedling raising substrate, and the two can be compounded and coexist, thereby realizing the synergistic effect of functional microorganisms.The application overcomes the defects of the existing biocontrol strain, such as reduced activity in a low temperature environment and unstable prevention effect, and provides a stable and reliable biological control scheme for factory seedling raising in a low temperature season without increasing the amount of chemical pesticides, thereby significantly improving the quality of seedlings and the seedling raising success rate.

The invention discloses a Trichoderma cererum and application thereof, the name of the Trichoderma cererum TR1186A is Trichoderma cererum TR1186A, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.21427. The Trichoderma cererum is named as Trichoderma cererum TR1186A. The Trichoderma cererum is named as Trichoderma cererum TR1186A. The trichoderma cereus provided by the invention is an effective biocontrol strain and can antagonize a tobacco black shank strain, and test results of a plate confrontation test, an indoor pharmacodynamic test, an indoor safety test, a field pharmacodynamic test and the like prove that the strain can be developed into a biological pesticide which is environment-friendly, high in safety and good in control effect; particularly, the bacillus subtilis has a good application prospect in biological prevention and control of tobacco black shank.

This invention relates to the field of fertilizers, specifically to an organic fertilizer containing *Penicillium oxalate* and *Trichoderma echinococcosis*, and its preparation method. The organic fertilizer, by weight percentage, comprises: 10%-25% *Penicillium oxalate*, 10%-25% *Trichoderma echinococcosis*, 10%-20% sheep manure, 5%-10% ammonium phosphate, 5%-10% potassium fertilizer, 10%-20% straw ash, 1%-5% glyphosate, 1%-5% 6-benzylaminopurine, with a binder to make up 100%. The active ingredients of this invention are *Penicillium oxalate* and *Trichoderma echinococcosis*. It is mainly used to increase the yield of vegetables, flowers, and other crops while preventing fungal diseases such as anthracnose and black rot.

The invention discloses a botanical alkaloid and trichoderma synergistic preparation and application thereof, and belongs to the technical field of biological control. The invention provides a preparation containing trichoderma haworthia and chelerythrine in order to solve the technical problems of long period, low prevention and treatment efficiency and easiness in causing harm to environment and human health in a tobacco mosaic disease prevention and treatment method in the prior art. According to the preparation provided by the invention, under the condition that the dilution multiple is 50, the control effect on the tobacco mosaic virus is improved through multi-mechanism synergism, the provided preparation can be applied to tobacco planting production, the control efficiency of the tobacco mosaic disease can be effectively improved, and the economic benefit of tobacco planting is improved.

The invention relates to the technical field of water body remediation, and particularly discloses a trichoderma sp. XH5, a hybrid system as well as a construction method and application of the trichoderma sp. XH5, the trichoderma sp. XH5 is preserved in China Center for Type Culture Collection on September 29, 2025, the preservation number is CCTCC: M 20252142, and the trichoderma sp. XH5 is classified and named as Trichoderma sp. XH5. The Trichoderma sp. XH5 provided by the invention has the capability of denitrification, and specifically, the removal rate of nitrate, total nitrogen and nitrite is improved.

A biological control method for centipede breeding belongs to the field of special economic animal breeding technology. This invention uses a compound probiotic strain of *Trichoderma longicornis*, *Streptomyces flavus*, *Bacillus subtilis*, *Bacillus licheniformis*, *Clostridium butyricum*, and lactic acid bacteria, which is fully incorporated into the breeding substrate. After 24-72 hours of static setting, the bacteria fully colonize and form a stable antibacterial state. The bacterial community can remain dormant during periods of nutrient deficiency, low-temperature overwintering, or year-end static setting, and will revive upon contact with organic matter. The method is supplemented with springtails and pink woodlice, which preferentially consume harmful bacteria, clean up uneaten feed, and primarily feed on and decompose centipede feces, forming a mutually beneficial symbiotic relationship with the biocontrol bacteria. Their feces further nourish the bacterial community, extending the stable effective period of the bacterial community from 2 months to 4-6 months or more. Simultaneously, probiotics and vitamins are added to the feed to strengthen the centipede's constitution. This invention constructs a three-dimensional synergistic prevention and control system that integrates matrix inhibition of pathogenic bacteria, environmental residue removal, and internal administration for enhanced protection. This system enables drug-free ecological disease control, significantly reduces the incidence of diseases such as green muscardine, and improves the survival rate of centipede farming. It is suitable for large-scale centipede farming.

The invention relates to the technical field of agricultural biological agents, and particularly provides trichoderma harzianum as well as a compound preparation and application thereof. The trichoderma harzianum has a remarkable inhibition effect on six common plant pathogenic bacteria such as sclerotinia sclerotiorum, botrytis cinerea and fusarium oxysporum. The fermentation liquor has a remarkable growth promoting effect on various plants such as pakchoi, spinach and flowering Chinese cabbage when being diluted by 10-100 times, and the fresh weight, the dry weight and the chlorophyll content of the plants can be increased. The compound liquid is formed by compounding trichoderma harzianum fermentation liquor and a hypecoum erectum alkaloid extract with the concentration of 50 ppm, the effective dilution interval of the trichoderma harzianum fermentation liquor in the compound liquid is 10-100 times, and the compound liquid has an inhibition effect on cowpea fusarium wilt germs, promotes growth of cowpeas and nicotiana benthamiana at the same time, achieves integration of disease prevention and growth promotion and has a good application prospect. Wide agricultural application prospects are realized.

The invention discloses application of trichoderma xerophyllum to prevention and treatment of plant root-knot nematodes, and belongs to the technical field of microorganisms, the trichoderma xerophyllum is preserved in Guangdong Microbial Culture Collection Center, the number is GDMCC No: 66977, and the preservation address is No.100, Xianlie Middle Road, Xiaolian District, Guangzhou City, Guangdong Province. The preparation method of the fungicide prepared based on the trichoderma xerophyllum comprises the following steps: transferring the strain into a seed culture medium, and culturing on a shaking table at the temperature of 25-30 DEG C and the speed of 150-200r / min for 24-36 hours to obtain a fermentation seed solution; inoculating the fermentation seed solution into a fermentation culture medium, and culturing for 72-96 hours on a shaking table at the temperature of 25-30 DEG C and the speed of 150-200 r / min to obtain a fermentation solution, namely the target fungicide. The trichoderma xerophyllum has a remarkable lethal effect on root-knot nematodes, the fatality rate of fermentation liquor of the trichoderma xerophyllum on the root-knot nematodes exceeds 90%, the fatality rate of 80% or above can still be kept even if the trichoderma xerophyllum is diluted by 50 times, the trichoderma xerophyllum acts quickly, nematodes can be quickly killed within 12 hours, larvae are effectively prevented from invading plants, and the trichoderma xerophyllum shows a good application prospect in the field of prevention and treatment of the plant root-knot nematodes.

The present invention relates to a method for producing disaccharides using beta-glucosidase and its cofactors, and a composition for inducing enzyme production in a Trichoderma strain, which contains the produced disaccharides. Specifically, the method comprises inducing beta-glucosidase and a divalent metal ion, manganese ion (Mn ion), as a cofactor, on a glucose substrate. 2+ ), magnesium ion (Mg 2+ ), zinc ion (Zn 2+ ) or copper ions (Cu 2+ ), the disaccharide production rate can be increased and disaccharides can be obtained at a high concentration. In addition, since the composition with a high content of disaccharides produced by the production method of the present invention has an excellent effect of inducing enzyme production in Trichoderma strains, it is possible to improve the enzyme productivity of Trichoderma by applying a fermentation process that utilizes the produced disaccharides at a high concentration.

The invention discloses trichoderma sp. Y3 and application thereof in promoting rice growth, and belongs to the technical field of microorganisms. The classification name of the trichoderma sp. Y3 is Trichoderma sp. Y3, the trichoderma sp. Y3 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2026214. And biological characteristics and molecular identification are combined to identify that the trichoderma Y3 belongs to a new trichoderma species. When the strain or a fermentation product thereof is applied to a rice culture medium, the growth of rice can be effectively promoted, and the plant biomass is increased. Trichoderma Y3 is developed and prepared into the biological bacterial fertilizer, and a new way is provided for application of microorganisms to development of crop production.

The invention discloses a special microbial compound fertilizer for improving quality and enhancing aroma of pandan leaves and a preparation method of the special microbial compound fertilizer, and belongs to the technical field of bacterial fertilizers. The microbial compound fertilizer is obtained by utilizing compound bacteria to ferment and treat waste, the compound bacteria are formed by compounding yarrowia lipolytica, trichoderma and bacillus subtilis, and the waste is formed by compounding pineapple leaves and banana stems. In the compound bacteria, yarrowia lipolytica, trichoderma and bacillus subtilis are compounded in the form of bacterial powder, and the mass ratio is (1-5): (1-2): (1-4). The preservation number of the yarrowia lipolytica is ACCC (China General Microbiological Culture Collection Center) 21176, and the preservation number of the trichoderma sp. Is ACCC 31526; the mass ratio of pineapple leaves to banana stems in the waste is 3: 4. When the microbial fertilizer is applied to pandan leaf planting soil, the aroma components of the pandan leaves can be remarkably improved, and a novel bacterial fertilizer is provided for improving the quality and aroma of the pandan leaves.

The invention relates to the various uses of a fungus strain which belongs to the Trichoderma genus and in which the cel1a gene has been knocked out. The invention relates in particular to a method of producing proteins by a fungus strain which belongs to the Trichoderma genus and in which the cel1a gene has been knocked out, comprising at least two steps:a first step of growth in a batch phase in the presence of at least one carbon growth substrate, anda second step of producing proteins in a fed-batch phase in the presence of a composition comprising at least lactose and a second sugar, the lactose content in the composition representing approximately between 0 and 30% by weight of the total sugar content of the composition, in particular between 5 and 30%.

The invention discloses a compound root humectant for improving the transplanting survival rate of cassava seedlings and a preparation method of the compound root humectant, and relates to the technical field of functional preparations for agricultural planting. Comprising 8-13 parts of a cassava homologous extract, 2-4 parts of trichoderma spore powder, 1-3 parts of an allicin derivative, 6-10 parts of modified konjac glucomannan, 11-17 parts of an intelligent water absorption component and 4-6 parts of a betaine-humic acid chelated nutrition component. Through the specific matching of the cassava homologous extract and the trichoderma spore powder-allicin derivative composite disease prevention component, and in combination with a customization process of low-temperature activation and targeted layered coating, the effectiveness of bioactive components is effectively retained, and the three-layer structure microparticles realize a closed loop of activity retention, precise attachment and step-by-step release, so that the disease prevention effect is improved. The inner-layer biological components play a role in root promotion and disease prevention after transplanting, the middle-layer intelligent water absorption components continuously lock water, and the outer-layer chelating nutrient components are slowly supplied, so that the absorption rule of thin and weak root systems of cassava seedlings is accurately adapted.