470 results about "Trichoderma sp." patented technology

Fungal / bacterial antagonist combinations, a seed coated with one of the combinations and a plant protected from plant pathogens by one of the combinations. The invention is also a fungal / bacterial antagonist combination comprising a Trichoderma virens fungal antagonist and a Bacillus subtilis var. amyloliquefaciens (Bacillus amyloliquefaciens) bacterial antagonist and its use for controlling plant pathogens as a biocontrol agent, bio-pesticide or bio-fungicide. The invention also finds utility as a fungal / bacterial antagonist combination applied to the seed, stalk or leaf that results in an increase in plant yield. Control of early and late season stalk and root rot caused by fungi such as Fusarium, Phythium, Phytophthora and Penicillium in tomatoes, peppers, turf grass, soybeans, sunflower, wheat and corn is achieved.

The invention relates to the field of environmental protection and discloses a soil conditioner, as well as a preparation method and application thereof. The soil conditioner comprises raw materials in parts by weight: 50 to 60 parts of wood fibers, 14 to 18 parts of biochar, 25 to 30 parts of humus, 3 to 6 parts of polysaccharide, 1 to 3 parts of seaweed essence, 0.001 to 0.005 part of Trichoderma and 0.001 to 0.005 part of nitrogen fixation bacteria. The invention also discloses a preparation method, the application and a using method of the soil conditioner, the soil conditioner has the advantages of improving soil physical and chemical properties and promoting the turf forming performance of plants, and the preparation and application methods are simple and convenient, so that the popularization and application are facilitated.

The present invention is one kind of soil improving agent for vegetable plot and its preparation process and application, and belongs to the field of soil improving microbe preparation technology. The soil improving agent consists of turf, diatomite, microbe preparation, peanut cake and fly ash, and its microbe preparation includes Bacillus subtilis fermenting liquid, actimomycetale fermenting liquid and trichoderma fermenting liquid in the weight ratio of 1 to 0.5-3.5 to 0.5-6. It is applied to soil after being mixed in the field, can improve soil, and raise crop yield and quality obviously.

The invention relates to a trichoderma atroviride preparation for preventing and controlling vegetable fungal diseases and a preparation method thereofof the trichoderma atroviride preparation. A trichoderma strain Tr1208 is trichoderma atroviride and preserved in the CGMCC (China General Microbiological Culture Collection Center), and the preservation number is CGMCC No. 6828. According to The the trichoderma atroviride preparation takes, the trichoderma atroviride Tr1208 is used as an effective active ingredient and is prepared by being matched with an agriculturally acceptable carrier and a protective agent for the preparation; the bacteria content of the effective active ingredient is (1.0-30.0)*108 spores / gram; and the bacteria protectant content is 0.05%-1.8%. The preparation can have the effects of effectively preventing and controlling leek Botrytis, cucumber brown patches, cucumber powdery mildew, cucumber downy mildew, tomato early blight, cucumber type damping off, cucumber type blight and cucumber type root rot and promoting plant growth and characteristics of efficiency, wide antimicrobial spectrum, simple usage, low cost, no environmental pollution and plant micro ecological environment improvement.

The invention discloses a trichoderma preparation for preventing and treating soil-borne diseases of crops and a preparation method thereof. The compositions of the preparation comprise that: a carrier comprises a useful quantity of Trichoderma harzianum strains Th-192 CGMCC NO.2634. The preparation method comprises the steps of propagation of the strains Th-192, liquid and solid fermentation culture, addition of an accelerant, and the like. The preparation has the advantages of wide sources of raw materials, short production period, low cost, large spore yield, convenient use, wide applicable crops and obvious functions of promoting growth and increasing production.

The invention relates to Trichoderma harzianum Rifai WRF-2 and use thereof. The bacterium strain was preserved in China General Microbiological Culture Collection Center on January 21st, 2009, with a preservation number of CGMCC No.2870. The bacterium belongs to deuteromycetes and is a lower fungus that is short in culture period and quick in growing and propagating speed and produces a large amount of uniform culture liquid for lignin degrading enzyme-generating bacteria in a short time compared with a major part of basidiomycetes to which whiterot fungi belong. The invention also provides a method for culturing and producing lignin peroxydase (Lip) and manganese peroxidase (MnP) by the fermentation of the bacterial strain in a liquid enzyme-generating culture medium, which can produce high-acitivity LiP and MnP in a short time, and is simple in fermentation process, stable, low in cost, and high in yield.

The invention discloses a microbial decomposition agent for animal wastes and straws and a preparation method thereof. The microbial decomposition agent comprises the following strains in parts by weight: bacillus, saccharomycetes, Aspergillus niger, Aspergillus oryzae, trichoderma sp, lactic acid bacteria, photosynthetic bacteria and actinomycetes. The decomposition agent has diversified functions, can be simultaneously used for composting straws and / or animal wastes, contains multiple functional bacteria and is high in microbe activity, the selected strains achieve a synergistic effect, do not generate antagonism, and the decomposition agent has the advantages of fast temperature rise and high temperature of fermented materials, complete decomposition, short decomposition time and the like. The preparation method of the microbial decomposition agent disclosed by the invention has the advantages of simplicity, feasibility, simple operation and low production cost.

The invention relates to a high-efficiency hardening-resisting continuous-cropping-resisting soil-modifying biological agent. The biological agent is composed of: 20% of trichoderma, 20% of streptomyces microflavus, 30% of starch, 10% of grass peat, 8.15% of a siliceous fertilizer, 6% of humic acid, 5% of urea, 0.2% of biological strains (including 1 / 3 of bacillus subtilis, 1 / 3 of bacillus cereus, and 1 / 3 of bacillus licheniformis), 0.1% of vitamin C, 0.5% of compound sodium nitrophenolate, and 0.05% of DA-6. With the soil-modifying biological agent provided by the invention, defects of existing fertilizers are overcome, the pollution to the environment is reduced, soil is activated, soil hardening and continuous-cropping are overcome, and substantial influences are provided for healthy growth and bumper harvest of crops.

The invention relates a special organic culturing substrate capable of preventing and treating diseases for peppers and the preparation method of the special organic culturing substrate. The special organic culturing substrate is prepared from the following raw materials: furfural residues, edible fungus residues, oil residues, saw dust, a microbial fermentation fungicide EM composite fungicide, a trichoderma fungicide and a bacillus fungicide, wherein the bacteria liquid of the trichoderma fungicide has the effective bacteria content of 2X10<8> / g-5X10<8> / g, and the bacteria liquid of the bacillus fungicide has the effective bacteria content of 2X10<9> / g-4X10<9> / g. According to the invention, different organic wastes are compounded, are fermented, decomposed and sterilized, then the trichoderma fungicide and the bacillus fungicide which have the function of preventing and treating diseases of pepper roots are added, compositing is carried out, and finally, the special organic culturing substrate is obtained. The special organic culturing substrate prepared by the preparation method contains a large amount of active trichoderma, bacillus and the metabolic products of the active trichoderma and the bacillus, has an obvious inhibition effect on the diseases of the peppers, and improves the quality and the yield of the peppers.

The invention relates to a novel microbial organic fertilizer with anti-actinomyces active compound bacteria and a preparation method thereof. The novel microbial organic fertilizer comprises a microbial inoculum and an organic fertilizer, wherein the microbial inoculum comprises colonies of Bacillus siliceous, Nocardia actinomyces, Bacillus subtilis, Bacillus thuringiensis, Brevibacillus laterosporus, azotobacter, Beauveria bassiana, Metarrhizium anisopliae, Trichoderma and Saccharomyces, and peptide proteins and rare-earth elements having unique effects on inhibiting wireworms and enhancing plant root system stress tolerancen; and the organic fertilizer comprises animal manure and plant fibers. The novel microbial organic fertilizer is prepared by fermenting the animal manure and plant fibers by using the microbial inoculum. The microbial organic fertilizer can quickly and effectively kill wireworm ova in soil, has strong effects on inhibiting and killing larvae and imagos, can continuously improve the growth environment of the plant root by using the growth of the microbial colonies and the stress tolerance of the peptide proteins, enables the plant to have strong roots, thick stems and green leaves, postpones the aging of the plant, and promotes prematurity and yield increase.

The invention relates to a preparation method of trichoderma multifunctional soil modifying agents. The preparation method comprises the steps that: trichoderma harzianum SH2303 with the CGMCC (China General Microbiological Culture Collection Center) NO. 4963 is cultured on a potato dextrose agar (PDA) culture medium for 2 to 3 days, a bacterial cake is punched by a hole puncher with the diameter being 5mm, the bacterial cake is inoculated into a seed culture medium for 5 to 7 days at the bacterium inoculation quantity of three tablets per bottle and is then inoculated into a spore production culture medium, the fermentation is carried out for 5 to 7 days under the optimized culture condition in a 300 L fermentation tank, and the number of thick-wall spores is (6 to 7)*10<8> / mL; and fermenting liquid and carriers are mixed according to the weight ratio of 1:1, the secondary open type solid fermentation is carried out for 5 to 7 days at the normal temperature, and the number of trichoderma conidiospores and chlamydospores is 200 to 500 million / g. The soil modifying agents prepared by the method have the main purposes of improving the primary and secondary salinization of vegetable planting soil and degrading the organic phosphorus pesticide residue soil, and have the effects of treating soil-borne diseases, promoting the crop growth and improving the crop yield. Because only one trichoderma strain is utilized, the fermentation process is easy to optimize, the microbial inoculum preparation cost is low, the commercial large-scale popularization is easy to realize, and high field general use values are reached.

The invention relates to a trichoderma pseudokoningii (SMF2) strain and application thereof, which belong to the technical field of microbiology. The invention discloses the trichoderma pseudokoningii (SMF2) strain preserved in China Center for Type Culture Collection on February 24, 2009 with the preservation number being CCTCCNO: M209031, and the application of the strain to preparation of soil microbial agents of trichoderma preparations. The trichoderma pseudokoningii (SMF2) strain disclosed by the invention can be used for producing plant disease fungus cell wall digestive enzymes (CWDEs) and antibiotics of peptaibols at high yield, the CWDEs and the peptaibols cooperate in situ, and the inhibition effect of the trichoderma pseudokoningii strain to the plant disease fungus is further enhanced.

The invention discloses a compound microbial fish fertilizer special for silver carp and a preparation method thereof. The compound microbial fish fertilizer special for silver carp comprises the following components in parts by weight: 0.5-0.8 part of silicate bacteria, 1-3 parts of streptococcus faecalis, 0.6-0.8 part of trichoderma sp, 2-5 parts of rhodopseudomonas palustris, 0.3-0.5 part of saccharomycetes, 50-60 parts of fishbone dust, 65-70 parts of vinegar residue powder, 50-55 parts of effluent precipitate, 40-60 parts of rice bran, 12-14 parts of brown sugar, 10-14 parts of table salt, 80-85 parts of pig manure, 45-55 parts of waste of a pig meat slaughter house, 60-65 parts of aquatic plants, 12-16 parts of calcium bicarbonate, 14-17 parts of urea, 1-3 parts of ferric citrate, 0.6-0.8 part of vitamin B1, 0.2-0.6 part of Vitamin B12, 1-3 parts of rheum officinale, 2-6 parts of Astragalus membranaceus and 4-7 parts of medicated leaven. The fertilizer disclosed by the invention can promote silver carp to grow quickly, the EPA content of fish is increased, the fish diseases are reduced, and the number of dead fish is reduced, and meanwhile, beneficial microorganisms in water are increased. Finally, both the quality and the weight of silver carp are improved.

The invention relates to a method for recyclingly producing a trichoderma sp. biological control agent from potato starch waste water and mushroom residues and belongs to the technical field of microbial pesticides and environmental protection. The method adopts high-concentration organic wastewater produced in potato starch production and mushroom residues as main medium raw materials and utilizes a liquid state-solid state combined fermentation production method to produce the trichoderma sp. biological control agent. The method comprises the following steps of 1, inoculating potato starch wastewater as a culture medium with a trichoderma sp. strain and carrying out liquid-state culture to obtain a liquid strain, 2, inoculating potato starch waste water as a fermentation raw material with the liquid strain to obtain a large number of liquid trichoderma sp. products, 3, adding the cultured liquid strain or the liquid trichoderma sp. products into mushroom residues, and carrying out solid state fermentation to obtain solid trichoderma sp. products, and 4, carrying out drying, crushing and packaging of the solid trichoderma sp. products. The method for producing trichoderma sp. biological control agent has the characteristics of low production cost and good environmental benefits. The trichoderma sp. biological control agent obtained by the method can control a plurality of fungal diseases of plants. The method provides an effective treatment approach for high-efficiency treatment on potato starch wastewater and mushroom residues, and reduces environmental pollution.

The present invention belongs to the technical field of crop organic biological fertilizer, in order to solve the increasingly seriously continuous cropping obstacle of vegetable planting, lighten the occurrence of disease and improve the output and the quality of the vegetable, biological soil additive which can prevent the disease and as well as increase the production is confected, comprising two microorganisms, nonmetal mineral, fermented organic fertilizer, industrial waste residue and mineral microelement. The components comprise the weight proportion as follow: 10 percent of trichoderma functional microbial agent, 2 percent of VA arbuscular mycorrhizal fungi, 6 percent of inorganic nonmetal mineral zeolite, 69 percent of fermented organic fertilizer, 4 percent of shell powder, 7 percent of calcium superphosphate and 2 percent urea. The present invention is widely applied to the vegetable planting at the protected field with seriously continuous cropping and successive cropping and has the functions of improving the soil, promoting the crop nutrient absorption, increasing the output and preventing the disease.

The present invention relates to Trichoderma atroviride strains and their use as biological control agents or plant growth promoters. Methods and compositions for biological control of soil borne plant pathogens, and increasing plant yield using T. atroviride are also provided.

The invention discloses a production method for a biological organic fertilizer containing medium trace element chelates of humic acid. The biological organic fertilizer belongs to the field of manufacturing of composite fertilizers. The production method comprises the following steps: with pig manure, human feces, cow dung, rabbit manure, vinasse, cassava residue, leftovers in production of monosodium glutamate, trench mud, corn straw, soybean straw, vetch straw, a rapeseed cake, a tea seed cake, a deodorant, a biological nitrogen fixation bacterium, a phosphate solubilizing bacterium, a potassium solubilizing bacterium, a nodule bacterium, actinomyces, a silicate bacterium, trichoderma sp., microzyme, a photosynthetic bacterium, calcium humate, zinc humate, magnesium humate, iron humate, copper humate, and manganese humate used as raw materials, carrying out tank continuous fermentation to allow the raw materials become thoroughly decomposed; carrying out deodorizing and removing water; and carrying out granulation to obtain the granular biological organic fertilizer containing medium trace element chelates of humic acid. The fertilizer has a complete range of nutrient elements and can fully satisfy needs of a crop for a fertilizer during growth.

The preparation method of organic ecological soilless culture medium belongs to the field of agricultural bio technology. It is made up by using 100 portions of straw powder, 50-60 portions of cumulose fermented livestock and fowl dried excrements and 3-5 portions of compound bacterial preparation formed from azotogen and trichoderma preparation through the processes of uniformly mixing and stirring. The preparation of azotogen adopts cellulose Nocardia HD-86 with functions of decomposing cellulose and fixing nitrogen as strain and adds the corn stover, bran and water, and utilizes the aseptic fermentation process to obtain the azotogen, and the preparation of trichoderma preparation uses pseudo-Kan's trichodermin S28 as strain, and uses cob, bran, ammonium sulfate as culture medium through the processes of pulverizing, mixing, moistening, inoculating S28 and fermentation so as to obtain the trichoderma preparation. Said invention is favorable for industrial production of vegetables.

The invention discloses a Trichoderma asperellum TC01. The Trichoderma asperellum TC01 is collected in the China General Microbiological Culture Collection Center, and the collection number is CGMCC NO. 3.19218. The Trichoderma asperellum TC01 has the remarkable inhibiting effect on a tea-tree anthracnose pathogenic bacterium Colletotrichum gloeosporioides and an apple valsa canker pathogenic bacterium Phytophthora plurivora, and antibacterial substances generated by the Trichoderma asperellum TC01 can also inhibit growth of the tea-tree anthracnose pathogenic bacterium and the apple valsa canker pathogenic bacterium. The trichoderma asperellum can also promote growth of tea seedlings, and is a strain having the good environmental safety, the biological control capacity and the growth promotion function.

The invention discloses trichoderma biological humic acid and a product thereof. The trichoderma biological humic acid comprises trichoderma mycelia, trichoderma spores, trichoderma metabolite, biological humic acid, biochemical fulvic acid, amino acid, nucleic acid, gibberellic acid, indolic acid, cellulose and xylogen. The trichoderma biological humic acid is prepared by the following methods: uniformly mixing one or more raw materials of straws, pot ale, wooden meal, pomace and livestock manure and crushing; then carrying out degradation by virtue of alkali or ammonia, so as to obtain a degraded product; adding a 0.02wt%-0.1wt% of a trichoderma agent and 50wt%-60wt% of water on the basis of weight of the degraded product, uniformly mixing, carrying out aerobiotic or aerobic fermentation at 20-35 DEG C for 5-7 days so as to prepare trichoderma biological humic acid, wherein the trichoderma agent is trichoderma harzianum or trichoderma viride. The trichoderma biological humic acid can replace chemical fertilizers, so that the consumption of pesticides can be reduced, water, the fertilizerand the pesticides can be integrated, the total amount of water, the fertilizer and the pesticides is saved by 20%-50%, soil can be effectively improved, soil organic matter is improved, the micro-ecological environment of soil is repaired, and the disease resistance and the adverse resistance of crops are improved, soil-borne diseases are inhibited, the successive cropping obstacle is prevented and controlled, the consumption of the chemical fertilizer and the pesticide can be reduced, and all the effects are obvious.

The invention discloses a manufacturing method and the application for a wood fungus conidium dust agent. It gains wood fungus from separating leaf of vegetables, purifying and transplanting to PDA test tube bevel to be used as mother fungus, adjusting temperature, pH value, and oxygen supplying quantity to produce spore liquid, and gaining large quantity of dry proofing conidium by adopting solid fermenting, mixing crashed condium and superfine diatomite to form wood fungus conidium dust agent. The method could be used to prevent vegetable producing.

The invention discloses a method for preparing a trichoderma fungicide, which is characterized by comprising the following steps: 1) carrying out seed culture on trichoderma strains; 2) diluting the trichoderma seeds obtained in the step 1) by using sterile water so as to obtain a trichoderma spore suspension; 3) carrying out liquid state fermentation on the trichoderma spore suspension obtained in the step 2) so as to obtain liquid trichoderma fermentation broth; and 4) placing the liquid trichoderma fermentation broth obtained in the step 3) in a destarched potato residues solid medium to carry out solid state cultivation. The method disclosed by the invention has the advantages that: 1, according to the technical scheme provided by the invention, easy-to-take and cheap destarched potato residues are adopted for producing the trichoderma fungicide, therefore, the problems of high cost and complex formula and the like are solved, and the problem that potato residues cause environmental pollution and can not be fully recycled is also solved; and 2, according to the technical scheme provided by the invention, the number of produced trichoderma conidia can be as high as 3.2*1012 cfu / g substrates, and the spore production quantity is 100 times as large as that in the prior art, thereby improving the output capacity, and further saving the cost.

The invention provides a Trichoderma wettable powder and a preparation method thereof. The method comprises the following steps: mixing Trichoderma liquid, a filling material and a raw powder protective agent, drying to obtain the Trichoderma raw powder, crushing an auxiliary agent containing a carrier, a dispersant, vitamin C, a sticker and a wetting agent and mixing; mixing the Trichoderma raw powder and the auxiliary agent, and drying to obtain the Trichoderma wettable powder. The method of the invention employs liquid fermentation, can be directly used for preparing the wettable powder; dextrin and vitamin C are added as the protective agents, viability stage of Trichoderma can be protected and prolonged under room temperature and drying condition, loss rate of Trichoderma is greatly reduced, shelf period of the product is increased; the raw powder and the auxiliary agent are separately prepared, Trichoderma spore mixing and stirring time can be reduced, Trichoderma spp loss rate is greatly reduced; the materials of the auxiliary agent are chemical substances, so that large scale production can be realized, and Trichoderma survival rate is not influenced.

This invention relates to a epiphyte co-culture ferment Laccase production method, which is to inoculate the bacterium detergent of the Trametes AH28-2(Trametes sp. AH28-2) in the ferment culture medium under 22-37deg C for 1-4 days, then adding the bacterium detergent of Trichoderma ZH1(Trichoderma sp. ZH1) to culture for 5-10 days, finally separating to obtain the Laccase preparation, wherein the enzyme activity is up to 6, 000U / L(guaiacol method). The culture medium is the liquid culture medium, which mainly contains a certain quantity of carbon, azotes, phosphate of kalium and natrium, sulfate of copper and ferrum, adenine and vitamin B and so on. This invention doesn't' use the aromatic compound and heavy metal iron induce, which is safe and clean, and the enzyme activity is high and stable. '

The invention provides trichoderma koningii with a collection number CGMCC No.9726. The invention also provides a compound microorganism bacterial agent; and the active ingredients of the compound microorganism bacterial agent include the trichoderma koningii, bacillus subtillis and bacillus amyloliquefaciens. The invention also provides application of the trichoderma koningii or the microorganism bacterial agent in the prevention of cylindrocarpon destructans. Through the technical scheme, the trichoderma koningii and the compound microorganism bacterial agent can effectively prevent cylindrocarpon destructans.

The invention relates to a method for cultivating a fungus capable of poisoning and killing plant parasitic nematodes and a method for preparing and using metabolites thereof, belonging to the technical field of microbial pesticides. The Trichoderma strain Snef85 involved in the present invention is Trichoderma harzianum, which has been preserved on October 30, 2007 in the General Microorganism Center of China Committee for the Collection of Microorganisms (address: Datun Road, Chaoyang District, Beijing, postal code 100101), The deposit number is CGMCC No.2235. The taxonomic status of the Trichoderma strain Snef85 is Deuteromycotina, Hyphomycetes, Trichosporales, Trichosporaceae, Trichoderma, and Trichoderma harzianum. It is prepared by liquid fermentation culture, and its metabolites have high toxicity to plant parasitic nematodes, especially root-knot nematodes incognita, rice stem-point nematodes and soybean cyst nematodes, and outstanding nematicidal effect. The fermentation broth of the strain has obvious control effects on root-knot nematode (Meloidogynes pp.), rice stem-point nematode (Aphelenchoides besseyi) and soybean cyst nematode (Heterodera glycines). There are significant differences between the treatment of fermented liquid with different dilution concentrations and the treatment of aseptic water, and the nematode killing effect of fermented liquid can reach more than 95%, which has good application and development prospects.

The invention discloses a microbial fertilizer, which comprises a microbial zymotic fluid consisting of photosynthetic bacteria, lactic acid bacteria, actinomyces, Corning trichoderma and a bacillus subtilis zymotic fluid in the volume ratio of 1:(0.8-1):(1.2-1.4):(0.6-0.8):(1-1.2). The utilization efficiency of the fertilizer prepared with the method can be increased, and the effects of preventing withering and plant diseases of plants can be achieved.

The invention provides a Trichoderma wettable powder and a preparation method thereof. The Trichoderma wettable powder is compounded from 10-10000 million CFU / g of Trichoderma, 1-40% of dispersant, 1-40% of wetting agent and 5-98% of filler. The preparation method comprises the following steps: pulverizing Trichoderma spore powder at low temperature, commonly pulverizing other assistants, and evenly mixing the Trichoderma spore powder and other assistants. The Trichoderma wettable powder is mainly used for preventing and treating soil-borne disease of vegetables and flowers, has favorable effect of preventing root rot of flowers, and seedling blight and cataplexis of vegetables, has the advantages of low toxicity and no residue, and can be used for producing organic food.