199 results about "Trichoderma sp." patented technology

The invention relates to the technical field of microorganisms, and discloses trichoderma yunnanense and application thereof. The Trichoderma yunnanense CCTCC NO: M 2025645 provided by the invention is a dominant strain of a high-yield siderophore, the activity of the siderophore is 69.79%, and the Trichoderma yunnanense can be used for synthesizing and secreting carboxylic acid type siderophore; the strain has a sulfur oxidation function; in addition, the invention also provides an application of the trichoderma sp. In regulation and control of plant diseases, the trichoderma sp. Disclosed by the invention can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae (Fr.) Kissler, and the trichoderma sp. Can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae (Fr.) Kissler, and can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae. The Trichoderma yunnanense provided by the invention is a strain separated from tobacco planting soil, and compared with a strain subjected to mutagenesis screening in an extreme environment and a laboratory, the Trichoderma yunnanense has better adaptability when being applied to a planting environment, and can be stably colonized in plant rhizosphere and soil, so that the Trichoderma yunnanense stably plays a growth promoting role for a long time, is safe and environment-friendly, and has a wide application prospect.

The invention relates to the technical field of microbial agents, in particular to a composite microbial agent and application of the composite microbial agent in prevention and treatment of forest root diseases, and the composite microbial agent comprises the following components in parts by weight: 15-25 parts of Trichoderma harzianum ATCC 20847; 10 to 20 parts of bacillus subtilis ATCC 605, 10 to 20 parts of bacillus subtilis ATCC 605, 10 to 20 parts of bacillus subtilis ATCC 606 and 10 to 20 parts of bacillus subtilis ATCC 607. 5-10 parts of a seaweed extract; 3 to 8 parts of chitosan; 2-5 parts of humic acid; 1 to 3 parts of glycine betaine; 0.5 to 1.5 parts of a trace element compound; 30 to 40 parts of perlite powder; according to the present invention, the fertilizer has characteristics of significant inhibition effect on a variety of forest root pathogenic bacteria, wide prevention and control spectrum, and long-term and stable disease prevention effect by adjusting the rhizosphere micro-ecosystem. Diseases are prevented and controlled, and the growth and root development of the forest trees are remarkably promoted.

The invention belongs to the technical field of soil improvement, and relates to a soil conditioner for saline-alkali soil and a soil improvement method. The soil conditioner comprises vinegar residues, chitin, glucose, a composite biological preparation, a modified gel and a functional additive, the composite biological agent comprises bacillus subtilis powder, bacillus licheniformis powder, trichoderma spore powder, brown algae powder and light calcium carbonate; the functional auxiliary agent comprises modified bentonite, polyglutamic acid, pregelatinized starch and biochar potassium humate. Through the synergistic effect of multiple components and structural design optimization, systematic remediation of physical properties, chemical properties and biological activity of the saline-alkali soil is achieved. According to the technology, the limitation of single function of a traditional improvement measure is broken through, and the long-standing technical problems of return salt rebound, low fertility, microbial community imbalance and the like in saline-alkali soil treatment are effectively solved by constructing a multi-layer and multi-dimensional soil improvement system.

The invention provides a control method for apple alternaria leaf spot, and belongs to the technical field of crop disease control. The prevention and treatment method comprises the following steps: spraying a foliar biological pesticide fertilizer to apple trees; the foliar biological medical fertilizer comprises a bacillus fermentation product and a trichoderma fermentation product, the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208, and the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208; the trichoderma is at least one of T6 and Ym. The combined action of bacillus and trichoderma is utilized, the morbidity of apple alternaria leaf spot can be effectively reduced, the growth of apple trees can be promoted, the photosynthetic capacity is improved, the combination of disease prevention and yield increase is realized, and the method is environment-friendly and suitable for green agricultural development.

The invention relates to the technical field of biocontrol microbial agents, and provides a preparation method of trichoderma particles. Comprising the following steps: inoculating and culturing trichoderma in a PDB culture medium, centrifugally collecting trichoderma thalli, embedding the trichoderma by using sodium alginate powder as a wall material, carrying out microencapsulation treatment on the trichoderma to obtain a microencapsulated trichoderma agent, drying the microencapsulated trichoderma agent at low temperature, and mixing and granulating an organic matter base fertilizer and the microencapsulated trichoderma agent to obtain the trichoderma particles. The trichoderma is subjected to microencapsulation treatment, so that the release time can be delayed, the influence on the activity of the trichoderma in the germination process of the coated corn seeds is avoided, and meanwhile, the trichoderma can be slowly released in the subsequent corn growth process to achieve a continuous biocontrol effect; the trichoderma particles prepared by the method are high in stability, a relatively high viable count of trichoderma can be still kept within a relatively long storage time, the storage time of the trichoderma particles is greatly prolonged, and the actually required long-time storage requirement is met.

The invention provides a trichoderma Guizhou B10 strain and application thereof, and relates to the technical field of agricultural microbial control. The trichoderma Guizhou B10 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 3.25634. The invention overcomes the defects in the prior art, and the strain can inhibit pathogenic fungi of malania oleifera leaf spot disease, such as pestalotiopsis, pestalotiopsis, clostridium neocarpum, botryosphaeria and physophaga fungi, can promote the growth of malania oleifera plants, and has both disease-resistant and growth-promoting functions on malania oleifera seedlings, such as malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, and malania oleifera leaf spot disease resistance.

The invention discloses a microbial agent and application thereof in agricultural production. The microbial agent is prepared by compounding seed solutions of two or more of seed solutions of Trichoderma harzianum, Trichoderma brevicompactum Tb12, Gliocladium roseum NF-06 and Trichoderma asperellum, and the seed solutions of the two or more of the Trichoderma harzianum, the Trichoderma brevicompactum Tb12, the Gliocladium roseum NF-06, the Trichoderma asperellum, the Trichoderma harzianum, the Trichoderma brevicompactum, the Gliocladium roseum NF-06 and the Trichoderma asperellum. The selected strain combination can effectively prevent and treat fungal diseases and nematode diseases and can promote plant growth. The trichoderma harzianum and the trichoderma brevicompactum Tb12 are used in a combined mode, the control effect on fusarium is obvious, the gliocladium roseum NF-06 and the trichoderma asperellum are used in a combined mode, the nematode killing effect is obvious, and the growth of rice is promoted through the trichoderma harzianum and the trichoderma asperellum.

The invention relates to a soil remediation complex microbial inoculant composition and a preparation method thereof, and belongs to the technical field of soil remediation. Comprising the following raw materials: 10-15 parts of a microbial complex inoculant, 40-50 parts of a porous carrier, 20-25 parts of an organic matter and 3-5 parts of a surface protective agent, wherein the porous carrier is obtained by treating diatomite with phytic acid and polylysine. Paenibacillus polymyxa, trichoderma harzianum, bacillus subtilis and azotobacter chroococcum are taken as a microbial compound inoculant, modified diatomite is taken as a porous carrier, and the microbial compound inoculant is mixed with organic matters under the action of a protective agent, so that the soil remediation compound inoculant composition which can effectively improve soil and is excellent in strain activity is obtained.

The invention provides application of trichoderma in relieving continuous cropping obstacles of ginseng or salvia miltiorrhiza, and relates to the technical field of crop cultivation. Through deep research and creative labor, the trichoderma can effectively relieve soil chemical property deterioration and soil microorganism change after continuous cropping of the ginseng or the salvia miltiorrhiza, so that the soil chemical property after continuous cropping of the ginseng or the salvia miltiorrhiza can be improved, the relative abundance of beneficial bacteria in the soil is increased, the relative abundance of harmful bacteria is inhibited, and the yield of the ginseng or the salvia miltiorrhiza is increased. Meanwhile, the content of effective components of ginseng and salvia miltiorrhiza can be increased, and the quality of continuous cropping ginseng or salvia miltiorrhiza is improved.

The invention discloses a trichoderma strain NAU-TL11-7 and an application of the trichoderma strain NAU-TL11-7 in oat growth and seed yield increase. According to the invention, a Trichoderma strain with a remarkable growth promoting effect is screened and separated, the strain NAU-TL11-7 is classified and named as Trichoderma lacumbens, and is preserved in the China Center for Type Culture Collection on November 15, 2024, the preservation address is Wuhan University, Wuhan, China, and the strain preservation number is CCTCC NO: M20242565. The trichoderma fertilizer product prepared by adopting the strain can obviously promote the growth of oat plants, improve the yield of oat seeds, reduce the application of chemical fertilizers in oat fields, realize the reduction and synergism of chemical fertilizers and the safe and efficient production of oats, and is an effective way for realizing the green and high-quality cultivation of artificial oat fields.

The invention belongs to the technical field of agricultural microbial control, and particularly relates to a growth-promoting and disease-preventing compound microbial agent and a preparation method and application thereof, and the compound microbial agent specifically comprises microbial powder, a growth auxiliary material and a drying agent; the microorganisms comprise vermiform yellow, trichoderma harzianum, bacillus velezensis, bacillus licheniformis and brevibacillus laterosporus. According to the compound microbial agent, through the synergistic effect of multiple strains, the control effect of the compound microbial agent on verticillium dahliae is enhanced, and the defects that a single biocontrol bacterium is single in biocontrol mechanism, poor in environment adaptability, incapable of being colonized in soil for a long time and unstable in control effect are overcome; the compound microbial agent can increase the yield of cotton and pepper and improve the quality of cotton fibers, and provides scientific and technological support and decision service for realizing green, environment-friendly, cost-saving and efficiency-increasing modern agriculture.

The invention belongs to the technical field of microorganisms, and particularly discloses trichoderma harzianum NBU-1 and application thereof. The Trichoderma harzianum NBU-1 is preserved in the Guangdong Microbial Culture Collection Center on March 26, 2025, the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCCNO: 66053. The invention discloses trichoderma harzianum NBU-1 and application thereof, the trichoderma harzianum NBU-1 is applied to prevention and treatment of wheat sharp eyespot, and the activity of rhizoctonia cerealis R0301 and the growth of scab are effectively inhibited.

The present invention relates to a composition comprising components (A) and (B) as active ingredients, wherein component (A) comprises a Streptomyces and component (B) is a compound selected from the group consisting of sulphur, phosphonate, copper hydroxide, copper oxychloride, bicarbonate, Bacillus sp, Trichoderma sp, aureobasidin A, jawsamycin, kasugamycin, natamycin, a polysaccharide elicitor, a plant biostimulant (PBS) comprising an algal extract, a plant extract and / or vinasse, orange oil, and / or tea tree oil. The invention further relates to a method of controlling or preventing phytopathogenic diseases, preferably phytopathogenic fungi, on a plant or on plant propagation material and / or on harvested food crops, which comprises applying to the plant, on plant propagation material, the locus thereof, and / or on harvested food crops the composition according to the present invention.

The invention discloses a trichoderma virens preparation capable of preventing diseases and increasing yield as well as a preparation method and application of the trichoderma virens preparation. Belongs to the technical field of biological agents. According to the trichoderma virens strain T259 disclosed by the invention, the trichoderma virens strain T259 is respectively subjected to strain amplification culture, liquid amplification culture and solid fermentation culture, a solid fermentation product of the T259 strain is successfully prepared, the solid fermentation product is mixed with an auxiliary agent, and the trichoderma virens preparation capable of preventing diseases and increasing yield is prepared. The trichoderma virens spore content in the preparation is improved; solid fermentation can effectively improve the colonization ability of trichoderma virens in the field. The production of the trichoderma virens preparation has the characteristics of large sporulation quantity, short period, wide raw material source and low cost; the fertilizer is easy to use and operate, suitable for various crops and remarkable in disease prevention and yield increase effect. And a foundation is laid for quality and efficiency improvement and high-quality and high-efficiency development of modern agriculture.

The invention belongs to the technical field of agriculture, and particularly relates to a fertilization method for improving the quality and yield of oat seeds, diammonium hydrogen phosphate and biochar are used as base fertilizers, the application amount of the diammonium hydrogen phosphate is 10-12 kg / mu, the application amount of the biochar is 10-15 kg / mu, bacterial fertilizer dressing is carried out in the seedling emergence period of crops, and chemical fertilizer dressing is not carried out in the later period; the bacterial fertilizer is an arbuscular mycorrhizal fungus inoculant and a trichoderma inoculant. The use amount of the chemical fertilizer is reduced, and the negative effect of excessive use of the chemical fertilizer on crop growth is reduced; the soil environment is improved by additionally applying biochar, and the nutrient utilization efficiency is improved; by utilizing a hypha network formed by arbuscular mycorrhizal fungi and root systems, the absorption area of the oat root systems is increased, and the nutrient utilization efficiency of plants is improved; the application of the trichoderma can promote the development of lateral roots of plants and accelerate the growth speed of the plants in the seedling stage; the fertilization method can improve the quality and yield of the oat seeds, is simple and easy to implement, is suitable for large-scale popularization and application, and has a wide market prospect.

The invention relates to the technical field of soil improvement, and particularly discloses a soil conditioner based on microbial community regulation and control and a preparation method thereof, the soil conditioner comprises: (a) a composite functional flora comprising nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium-dissolving actinomycetes and trichoderma, and the viable count ratio of the nitrogen-fixing bacteria to the phosphate solubilizing bacteria to the potassium-dissolving actinomycetes to the trichoderma is (1.0-1.5): (0.8-1.2): (0.5-1.0): (0.3-0.8); b) modified biochar carrier: the modified biochar carrier is prepared by oxygen-limited thermal cracking of agricultural wastes and is subjected to surface modification by a chitosan solution with a mass fraction of 3-5%; (c) a synergistic component which comprises humic acid particles and an enzymolysis seaweed extract; (d) a flora activator: compounding fructo-oligosaccharide and L-aspartic acid according to a mass ratio of 2: 1; through the synergistic effect of multifunctional composite flora of nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium-solubilizing actinomycetes and trichoderma, the biotransformation process of nitrogen, phosphorus and potassium elements in soil is synchronously activated, and the modified charcoal carrier is subjected to multi-stage pyrolysis and chitosan modification to form a graded pore structure.

The invention relates to the technical field of agricultural microorganisms, and discloses a complex microbial inoculant for improving soil micro-ecology and promoting plant growth and application thereof.The complex microbial inoculant is prepared from 30-40 parts of bacillus subtilis, 20-40 parts of pseudomonas, 10-20 parts of nitrogen-fixing bacteria, 5-10 parts of photosynthetic bacteria, 5-10 parts of trichoderma and 5-10 parts of penicillium; wherein the bacillus subtilis is bacillus subtilis L11 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.31274, the preservation date is July 11, 2024, and the bacillus subtilis is classified and named as bacillus subtilis. The complex microbial inoculant for improving the soil micro-ecology and promoting the plant growth is adopted, a multi-bacterium synergistic community is constructed, the complementary metabolism advantage of different strains at the rhizosphere is utilized, the nutrient conversion and stress resistance characteristics are improved, and therefore the soil micro-ecology is improved, and the plant root development is promoted.

The invention discloses a special disease-resistant compound microbial fertilizer for tea trees and a preparation method, and belongs to the technical field of agricultural fertilizers. The bacterial fertilizer consists of a microbial flora, a macro-element fertilizer, a trace element fertilizer and a carrier, wherein the microbial flora contains bacillus, trichoderma and actinomycetes. The preparation method comprises the steps of strain culture, fermentation, drying, crushing and mixing. Through the synergistic effect of various microorganisms, the bacterial fertilizer forms a protective barrier on tea tree root systems, inhibits growth of pathogenic bacteria, induces tea trees to generate resistance and enhances the disease resistance of the tea trees; the fertilizer is rich in various nutrient elements, meets the growth requirements of tea trees and promotes the growth of the tea trees; the soil structure is improved, the soil fertility is improved, and soil sustainable utilization is achieved; the content of nutritional ingredients such as tea polyphenol and amino acid in the tea can be improved, and the tea quality is improved. A plurality of embodiments verify that the bacterial fertilizer can significantly reduce the incidence rate of tea cake disease and tea anthracnose, increase the germination number and length of new shoots of tea trees, improve the amino acid content of tea leaves and the like, and has a good application effect.

The invention relates to a preparation method of compound microorganism bacterium soil, microorganisms in the compound microorganism bacterium soil comprise bacillus subtilis YCBA0319 with the preservation number of CGMCC No.33006 and trichoderma longibrachiatum YCFA0522 with the preservation number of CGMCC No.41655. The preparation method comprises the following steps: selecting relatively clean clay or loam, and removing stones and plant residues; mixing the fine soil, the soybean meal and the cottonseed cake, stirring by using a double-shaft paddle type mixer, and spraying sterile water to adjust the water content; sub-packaging into polypropylene woven bags, sterilizing, and cooling for later use; preparing a seed solution of the bacillus subtilis YCBA0319; preparing a spore suspension of the trichoderma longibrachiatum YCFA0522; the bacillus subtilis is 2.5 * 10 < 9 > CFU / g, and the trichoderma longibrachiatum is 5.0 * 10 < 9 > CFU / g. According to the technical scheme, a granular structure is formed with soil in the fermentation process, so that an appropriate living microenvironment is provided for microorganisms, effective microbial communities are protected from being influenced by external adverse conditions, and the activity and function exertion of the effective microbial communities after field application are ensured.

The invention provides a trichoderma fungicide for promoting growth of allium plants and application of the trichoderma fungicide, the trichoderma fungicide is a trichoderma harzianum strain MCBC3, the trichoderma fungicide and diammonium phosphate are applied in a combined mode, the application amount of the diammonium phosphate is 15 kg / mu, and the application amount of the trichoderma fungicide is 1 L / mu; existing trichoderma resource development focuses on a normal soil environment, the trichoderma harzianum MCBC3 is screened out from the saline-alkali soil for the first time, the salt resistance and the alkali resistance of the trichoderma harzianum MCBC3 directly aim at double stress of high salt and high alkali of the saline-alkali soil, and the technical bottlenecks that a traditional strain is poor in adaptability and low in survival rate in an extreme environment are solved.

The invention discloses a trichoderma complex microbial inoculant capable of promoting wheat growth and preventing and treating wheat stem rot and application of the trichoderma complex microbial inoculant. The trichoderma complex microbial inoculant comprises a trichoderma asperellum strain XX-57 and a trichoderma harzianum strain PY-127, and the preservation number of the trichoderma asperellum strain XX-57 is CGMCC No. 41937; the preservation number of the trichoderma harzianum strain PY-127 is CGMCC (China General Microbiological Culture Collection Center) NO. 41938. The invention further specifically discloses application of the trichoderma complex microbial inoculant in promoting wheat seed germination and wheat growth and preventing and treating wheat basal stem rot, the trichoderma complex microbial inoculant is applied 10-20 days after wheat seedling emergence, and it is found that compared with single trichoderma inoculation, the trichoderma complex microbial inoculant has the promoting effect on wheat plant height, root length, SPAD value, fresh weight and dry weight; the control effect on wheat stem rot is better, and particularly the control effect is more obvious when pathogenic bacteria are inoculated in advance.

The application discloses application of Trichoderma asperellum strain YN4 and fungicides in prevention and treatment of plant fungal diseases. The application research shows that the Trichoderma asperellum strain YN4 has a good inhibitory effect on pepper anthracnose, rubber anthracnose, mango anthracnose, soybean anthracnose and the like. Further, compared with the single use of fungicides or the Trichoderma asperellum strain, the inhibitory effect of the combination of the two is better. Under certain conditions, the Trichoderma asperellum strain is combined with 10% difenconazole water dispersible granules, 70% thiophanate-methyl wettable powder or 450g / L prochloraz emulsion in water, and has a synergistic effect. Not only can the use amount of fungicides be greatly reduced, but also the inhibitory effect on plant pathogenic fungi can be further improved, the incidence can be reduced, the inhibition on pathogenic bacteria can be enhanced, the use and residue of chemical pesticides can be reduced, and technical support is provided for the selection of fungicides for pepper anthracnose and the like and the combination of bacteria and drugs.

The invention discloses an application of graphene oxide in degrading straws by synergistic trichoderma. The application comprises the following steps: crushing the straws, and mixing the crushed straws with soil; spraying the trichoderma suspension into the mixture of the straws and the soil; spraying the graphene oxide hydrosol into a mixture of trichoderma, straws and soil; the concentration of the trichoderma suspension is 0.5 * 10 < 5 > cfu / mL to 5 * 10 < 5 > cfu / mL; the concentration of the graphene oxide hydrosol is 5 mg / L-50 mg / L; the water content of the mixture of the trichoderma, the straw and the soil is 50-70 wt%; trichoderma is treated through graphene oxide hydrosol, graphene oxide can promote mycelial growth of the trichoderma, meanwhile, lignin peroxidase activity and cellulase activity of the trichoderma can be promoted, and the straw degradation efficiency is improved; the expressions in the aspects of straw phenotype, mass loss rate, mycelial growth, cellulase and ligninase activity in the straw degradation process are greatly improved.

This invention relates to a composition comprising an active ingredient A and an active ingredient B, wherein the active ingredient A comprises a phosphorus-based compound, and the active ingredient B comprises a compound selected from Trichoderma sp.

The invention relates to the technical field of kitchen waste treatment, and particularly discloses compound bacteria and application thereof in kitchen waste recycling. The compound bacteria disclosed by the invention comprise a component A and a component B, the component A is formed by mixing bacillus velezensis, bacillus amyloliquefaciens, pseudomonas, bacillus atrophaeus, brevibacillus laterosporus and trichoderma harzianum; and the component B is prepared by mixing bacillus parlicheniformis, bacillus licheniformis, urea bacillus thermophilus and fat bacillus thermophilus. According to the technical scheme of resourceful treatment on the kitchen garbage by using the compound bacteria provided by the invention, the degradation effect of the kitchen garbage can be effectively improved, and the purposes of reduction, harmlessness and resourceful treatment of the kitchen garbage and energy consumption reduction in the treatment process are achieved; and the kitchen waste resourceful treatment material has an excellent application effect as a soil conditioner and a feed.

The invention discloses an application of trichoderma griseaginii TR2122 in prevention and treatment of beet root rot, a strain of the trichoderma griseaginii TR2122 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.21025, and the strain has strong antagonism and hyperparasitism effects on fusarium oxysporum causing the beet root rot. Meanwhile, the invention further provides a microbial preparation containing the live spores of the strain, the spore content is not lower than 2 * 10 < 8 > CFU / g, and the microbial preparation is preferably wettable powder containing a parent drug, a dispersing agent, a wetting agent and filler. In addition, the invention further discloses a preparation method of the preparation, and the preparation method comprises the steps of fermentation culture, concentration drying and mixing preparation. The strain and the preparation have stable and lasting control effect on beet root rot, are superior to or equivalent to conventional chemical agents, are safe and environment-friendly, and provide an effective solution for beet green control.

The invention discloses a preparation method and a use method of a sorghum biological seed-dressing agent, the biological seed-dressing agent comprises a seed-dressing agent 1 and a seed-dressing agent 2, the preparation method comprises the following steps: (1) mixing bacillus subtilis, trichoderma and corn flour, adding water, stirring into paste, and culturing at 25-30 DEG C for 48-60 hours to obtain thalli; (2) kneading the thalli into cubes, drying at room temperature, and grinding to obtain a seed dressing agent 1; and (3) uniformly mixing sodium alginate with water to obtain a seed dressing agent 2. The biological seed dressing agent can significantly improve the emergence rate of sorghum seeds, promote the growth and development of seedlings, and enhance the smut resistance of sorghum plants.

The invention relates to the technical field of fertilizers, in particular to a microbial amino acid-containing water-soluble fertilizer and a preparation method thereof. The method comprises the following steps: providing a first corn steep liquor and a second corn steep liquor; adding a Trichoderma harzianum-Purpureocillium lilacinum complex microbial inoculant and lactic acid bacteria into the first corn steep liquor, and carrying out first fermentation to obtain a first fermentation broth; mixing the first fermentation liquor with the second corn steep liquor to obtain mixed corn steep liquor; adding bacillus subtilis into the mixed corn steep liquor, and carrying out second fermentation to obtain second fermentation liquor; and sequentially adding a nitrogen fertilizer, a phosphate fertilizer and a potash fertilizer into the second fermentation liquor to obtain the water-soluble fertilizer containing microbial amino acids. The microbial amino acid-containing water-soluble fertilizer prepared by the preparation method provided by the invention can effectively improve the yield and quality of crops, improve the soil structure, enhance the soil fertility, promote the root development of the crops and improve the resistance of the crops to adversity.

The invention relates to the technical field of orchard planting and soil fertilization management, and discloses a mango orchard ecological fertilization planting method with root zone annular drip covering and inter-row pasture mixed sowing, which comprises the following steps: field foundation pretreatment: field shallow rotation no-tillage, ecological ditch digging and biological herbicide spraying; paving a domain composite covering layer constructed by root domain composite covering and intelligent drip irrigation, clamping a drip irrigation ring, and controlling drip irrigation by a sensor; mixed sowing of the ternary pasture between the rows in the rainy season is conducted, and the ternary pasture is cut and utilized in good time; after composting the mushroom dregs, the mushroom dregs of the double-microbial-agent synergistic compost, the straws and the trimmed mango tree branches and leaves, adding double microbial agents of bacillus subtilis powder and trichoderma powder, and precisely fertilizing; and precise fertilization, ecological disease prevention and control and field maintenance: irrigating roots with trichoderma in a flowering period, and maintaining drip irrigation and a covering layer. According to the mango garden ecological fertilizing planting method with root domain annular dripping covering and inter-row pasture mixed sowing, the soil fertility can be improved, diseases can be prevented, and the quality and yield of mangoes can be improved.

The present application relates to a kind of bacteria inhibiting and growth promoting microorganism and its compound microbial inoculant, preparation method and application.The present application provides a kind of bacillus subtilis (Bacillus subtilis) YCBA0319, preservation number is CGMCC No.33006;A kind of trichoderma longibrachiatum (Trichoderma longibrachiatum) YCFA0522, preservation number is CGMCC No.41655.In the technical solution of the present application, through bacillus subtilis and trichoderma longibrachiatum and the compound microbial inoculant of its composition, through the synergistic effect of two strains, it has the promotion effect to soil health and apple yield, quality etc., compared with control, soil bulk density is reduced, organic matter and available nutrient are improved, and apple single fruit size, yield and quality are all improved.