92 results about "Mortierella" patented technology

A method for producing highly unsaturated fatty acids comprising culturing a microorganism, belonging to the genus Mortierella and having resistance to a carbon source, in a medium having a carbon source concentration of at least 4% by weight, and collecting highly unsaturated fatty acids from the cultured products. Culturing the microorganism for about a week gives at least about 7 g / L of highly unsaturated fatty acids.

A method for producing highly unsaturated fatty acids comprising culturing a microorganism, belonging to the genus Mortierella and having resistance to a carbon source, in a medium having a carbon source concentration of at least 4% by weight, and collecting highly unsaturated fatty acids from the cultured products. Culturing the microorganism for about a week gives at least about 7 g / L of highly unsaturated fatty acids.

According to the invention, delta-12 desaturase gene (FADS12, d12) from mortierella alpine ATCC32222 (M. alpina ATCC32222) is amplified, such that multi-copy expression plasmid with inserted opai / d12 co-expression gene and recombinant Yarrowia lipolytica yeast strain are prepared. According to the invention, the recombinant Yarrowia lipolytica yeast strain assists in increasing endogenous substrate linoleic acid (LA) amount and outer soybean oil increased substrate LA amount in fermentation medium, through expressing d12. In the strain, opai gene and d12 gene expression and transcript levels are increased with a multi-copy integration manner, such that conjugated linoleic acid t10 and c12-CLA specific isomer synthesis amount in recombinant Yarrowia lipolytica yeast strain can be further improved.

The present invention relates to a new microbial process for the preparation of the compound formula (I)from a compound of general formula (II)wherein R stands for an alkali metal or ammonium ion, by the submerged cultivation of a mold strain able to 6beta-hydroxylate a compound of the Formula (II) in aerobic fermentation and by the separation and purification of the product of Formula (I) formed in the course of the bioconversion. The process comprises cultivating a strain of Mortierella maculata filamentous mold species that is able to 6beta-hydroxylate a compound of the general Formula (II), on a nutrient medium containing assimilable carbon and nitrogen sources and mineral salts and separating the product formed from the fermentation broth, then isolating the compound of formula (I) and purifying the same. Novel strains of Mortierella maculata are also disclosed.

The invention relates to a method for preparing arachidonic acid (ARA) by utilizing Mortierella alpine mycelia, comprising the following steps: first carrying out deep culture on the inoculation amount of the Mortierella alpine in a constant temperature shaker; transferring and inoculating the Mortierella alpine into a container with fermentation media, obtaining fermented liquor after culturing; filtering the fermented liquor, collecting mould organisms, washing, drying and grinding the collected mould organisms, extracting or pressing the mould organisms with a nonpolar solvent, harvesting oil and fat and further processing the harvested oil and fat to obtain the target product, wherein, parts of commonly used nitrogen sources of growth media or / and fermentation media are substituted by fungal meal of Mortierella alpine and the substitution ratio of the nitrogen sources in the fungal meal of Mortierella alpine is 10-90 by mass. The residual solid matter after extracting the mould organisms with the nonpolar solvent is the recyclable fungal meal of Mortierella alpine. The wastes generated in the process of producing the ARA by utilizing the Mortierella alpine by fermentation can be recycled, thus avoiding discharge of the environmental pollutants.

The invention relates to a mortierella alpine CCFM442 strain and use thereof. The mortierella alpine CCFM442 strain is used for producing thalli feed additive, the method for producing the thalli feed additive comprises the steps of slant cultivation, seed cultivation, fermentation cultivation and post treatment. The thallis feed additive is high in security. The fatty acid in the thallis feed additive is reasonable in compositions, the content of the polyunsaturated fatty acid in the total fatty acid achieves 42.4-45.8%, omega-6 / omega-3 ratio is 4.5-5.3, and meets the recommendation standard of WHO and FAO. Cheap glucose and bean pulp which are low in market price and easy to obtain, are adopted asa culture medium for producing the thallis feed additive, so that the production cost is low.

The invention discloses a method for expression of fatty acid desaturase by an acellular protein synthesis system, and belongs to the enzyme engineering field. The method uses the wheat germ acellular protein synthesis system for cloning and expression of omega 3 desaturase gene (FADS15) from mortierella alpine ATCC32222 (American type culture collection32222) without the need for preparation of mRNA, the expression amount is high, subsequent purification steps are simple, and the method lays the foundation for the next step of research of membrane protein crystal structures and functions.

It is intended to provide a breeding method whereby a lipid-producing fungus belonging to the genus Mortierella can be effectively and efficiently bred without resort to antibiotics. Transformation is carried out with the use of an auxotroph (for example, uracil auxotroph) of a fungus belonging to the Mortierella as a host. More specically speaking, a gene compensating for the auxotrophy, which is employed as a marker, is transferred into the host (the gene transfer step). Next, a transformant is selected by using the recovery from the auxotrophy as the marker (the selection step). The lipid-producing fungus may be M. aplina, for example.

The invention relates to a method for cultivating caterpillar fungus by using a ginseng type extract and application. According to the method, in a caterpillar fungus seed cultivation and fermentation cultivation process, the ginseng type extract is added into a culture medium for continuous cultivation and fermentation according to a conventional caterpillar fungus process. The caterpillar fungus can be agamotype Chinese mortierella, teleomorph Chinese mortierella, cephalosporium sinensis, tremellaceae, Oznyun sp or paecilomyces hepialid. The cultivation method provided by the invention is simple and convenient; the quantity of the needed ginseng extracts is small, and the cost is low; mycelium of a strain cultivated by the cultivation method is strong and can grow fast; the content of cordycepin in the mycelium obtained by fermentation is high; fermentation liquid and the mycelium which are cultivated by the method can be used for producing health-care caterpillar fungus products.

The invention relates to a mortierella alpine mutant strain ZR36 and applications thereof. Mortierella alpine ZR36 is preserved in China Center for Type Culture Collection (CCTCC) on 13th, September, 2013; preservation address is Wuhan university, Wuhan, China; and preservation number is CCTCC No.M2013419. The invention also provides applications of mortierella alpine ZR36 in production of arochidonic acid oil with low long-chain saturated fatty acid content. The content of long-chain saturated fatty acids, especially the content of long-chain saturated fatty acids containing more than twenty carbon atoms, in the arochidonic acid oil prepared using mortierella alpine ZR36 CCTCC No.M2013419 is relatively low (the total content of the long-chain saturated fatty acids containing more than twenty carbon atoms is less than 15wt%), so that freezing point of the obtained arochidonic acid oil can be reduced significantly, crystallization separation of saturated fatty acids caused by solidification of the arochidonic acid oil at relatively low temperatures is impossible, and oil quality is relatively high.

The invention provides a recombinant mortierella alpina strain Ma-oPaFADS17 with heterologous expression coming from pythium aphanidermatum Omega-3 desaturase genes and a method for constructing the mortierella alpina strain with heterologous expression coming from pythium aphanidermatum Omega-3 desaturase genes. The method adopts mortierella alpine uracil auxotrophic strain CCFM 501 as materials and uses an agrobacterium tumefaciens mediated genetic manipulation technology to obtain a recombinant strain with a high yield of EPA in the environment of normal temperature, wherein the yield of EPA reaches 617.1 mg / L, and the content of the EPA accounts for 18.7 percent of the total amount of fatty acid, which has an important meaning for the basic research and product development of oil-producing fungus mortierella alpine.

It is intended to provide a breeding method whereby a lipid-producing fungus belonging to the genus Mortierella can be effectively and efficiently bred without resort to antibiotics. Transformation is carried out with the use of an auxotroph (for example, uracil auxotroph) of a fungus belonging to the Mortierella as a host. More specically speaking, a gene compensating for the auxotrophy, which is employed as a marker, is transferred into the host (the gene transfer step). Next, a transformant is selected by using the recovery from the auxotrophy as the marker (the selection step). The lipid-producing fungus may be M. aplina, for example.

The invention discloses a fungus mortierella strain for straw decomposition in cold regions and an application of the fungus mortierella strain in decomposition of rice straw, and belongs to the fieldof isolation and application of fungi for straw decomposition in the cold regions. The invention discloses the isolated fungus mortierella strain which is used for straw decomposition in the cold regions for the first time and has microbial preservation number of CGMCC No.15188, and further discloses the application of the fungus mortierella strain for straw decomposition in cold regions in decomposition of the rice straw. The isolated fungus mortierella strain for straw decomposition in the cold regions is a cold-resistant microorganism, and has high cold resistance, higher cellulose decomposition capability and good fermentation effect on the rice straw at low temperature. The isolated fungus mortierella strain for straw decomposition in the cold regions has application prospect in preparation of straw decomposing agents, especially rice straw decomposing agents.

The invention discloses a single-carrier polycyclic aromatic hydrocarbon mixed bacterial agent with low temperature degradation-resistant effect and a preparation method and application thereof. The single-carrier polycyclic aromatic hydrocarbon mixed bacterial agent is prepared from a mixed bacterial agent and a corn cob which is used as a carrier, wherein the mixed bacterial agent is obtained bymixing pseudomonas SDR4 and mortierella alpine JDR4 which are screened from frozen and thawed soil according to a volume ratio of 1:1. The single-carrier polycyclic aromatic hydrocarbon mixed bacterial agent with low temperature degradation-resistant effect has the advantages that the biological activity is good, the cost is low, the preparation technology is simple, the secondary pollution is avoided, the recycling is not needed, the malicious competition, devouring and poisoning to microorganisms by indigenous flora, bacteriophage and toxic matters can be effectively shielded, and the highefficiency can be stably realized in the complicated environment; the effect of restoring the frozen and thawed soil which is polluted by polycyclic aromatic hydrocarbon in cold areas is obvious.

The invention relates to a mortierella alpine strain for producing arachidonic acid, belonging to the technical field of bioengineering. The strain is classified and named as mortierella alpine JN168, and is preserved in China center for type culture collection (CCTCC) with the CCTCC No. M2012073. The mortierella alpine strain for producing the arachidonic acid is obtained by separating and screening soil in forests of Hyesan in Wuxi of Jiangsu Province, is stable in characters and can produce the arachidonic acid with high yield by a fermentation method.

The invention relates to the field of pesticide compounding techniques, particularly relates to a penconazole and zhongshengmycin-containing sterilization composition, and also relates to an application thereof. The penconazole and zhongshengmycin-containing sterilization composition comprises effective ingredients of penconazole and zhongshengmycin with a weight part ratio of (90:1)-(1:90), and the penconazole and zhongshengmycin-containing sterilization composition is prepared into a wettable powder, a suspending agent, an emulsion in water, a water dispersible granule, emulsifiable concentrate and the like after being added with assistants and an excipient, and is used for controlling fungal diseases caused by germs such as erysiphe, uromyces, mortierella, septoria and venturia and bacterial diseases caused by germs such as Gram-positive bacteria, Gram-negative bacteria, mycobacteria and saccharomycetes. The penconazole and zhongshengmycin-containing sterilization composition has a relatively good synergism within a certain range, is superior to a single preparation on the aspect of control effects, is relatively durable in pesticide effect, has a special effect on controlling powdery mildew, rust diseases, scab, leaf spot diseases, gray mold, white rot, soft rot, streak diseases, rice blast, ring spot, gibberellic diseases and bacterial diseases, is relatively low in residue, is relatively easily decomposed after a lasting period, is relatively environment-friendly, and is beneficial to the increase of yield and income in an agricultural district.

A method of breeding a lipid-producing strain belonging to the genus Mortierella. According to the method of breeding a lipid-producing strain which involves an expression-inhibiting step of inhibiting the expression of a specific gene in the lipid-producing strain as described above, a lipid-producing strain belonging to the genus Mortierella can be efficiently and effectively bred.

The present invention relates to a process for producing arachidonic acid. In one embodiment, Mortierella sect. schmuckeri microorganisms are cultured in fermentation medium, preferably containing a component of a complex nitrogen source. Further disclosed is a food product which includes Mortierella sect. schmuckeri microorganisms or lipid isolated from such microorganisms to enhance the arachidonic acid content of the food product.

It is intended to provide novel compositions usable in improving the flavor of an alcoholic beverage made from grapes typified by wine. Namely, a composition usable in improving the flavor of an alcoholic beverage made from grapes which contains the culture of a strain belonging to a genus Aspergillus, Penicillium, Rhizopus, Rhizomucor, Talaromyces, Mortierella, Cryptococcus, Microbacterium, Corynebacterium or Actinoplanes and being capable of producing diglycosidase.

The invention relates to a microbial agent. The microbial agent is formed by three fungi including mortierella, trichoderma asperellum and mucor circinelloides. The fungi are activated, propagated anddiluted into 1-9*10<7>CFU / ml fungal liquid respectively, and the three types of fungal liquid are subjected to isovolumetric mixing to obtain the microbial agent. The application of the microbial agent to passivation of heavy metals in soil refers to a method of plant infection with the microbial agent to form infected okra, alfalfa and soybean plants, and removal rates of copper, zinc, lead, cadmium and manganese is 75.69%, 57.60%, 89.61%, 82.83% and 68.77% respectively. Preparation and application methods of the microbial agent are simple and economical, soil physiochemical properties can be effectively improved, fertilizer and water retention capacity of soil is increased, secondary pollution is avoided, and a short application period is realized.

The present invention relates to an acyl-CoA synthetase homolog protein from microorganisms of the genus Mortierella, a polynucleotide encoding the protein, and so on. The invention provides polynucleotides comprising an acyl-CoA synthetase homolog protein gene from, e.g., Mortierella alpina, expression vectors comprising these polynucleotides and transformants thereof, a method for producing lipids or fatty acids using the transformants, food products containing the lipids or fatty acids produced by the method, etc.

The invention relates to a method for planting and greening of tailing by a microbial agent and a modifier. The tailing is covered with soil dressing with the thickness of 2-4cm, the modifier made ofcharcoal, organic fertilizer, husk and haycite is applied, wherein the dosage is 2.0-2.5kg / m<2>; turning cover is conducted, wherein the depth of turning over is 15-20cm; after turning over, the microbial agent containing mortierella, trichoderma asperellum and mucor circinelloides is sprayed, wherein the dosage is 15-20g / m<2>; leveling and watering are conducted, the water holding rate of the tailing is 60%+ / -5%; and okra, alfalfa and kochia scoparia are planted, harvesting is conducted after 2-3 months. The method solves the problem that mass waste tailing produced by mining is hard to use.The adopted microbial agent is screened from the tailing, adaptation is achieved. The modifier is a green and non-polluting material, and the cost is low. Soil utilization of the tailing is achieved,and secondary pollution in the process of tailing utilization can be eliminated.

The invention relates to a mortierella alpina, M. alpina genetic engineering strain of an overexpression omega 3 desaturase gene (FADS15) and a construction method of the strain. According to the invention, a mortierella alpina, M. alpina uracil nutritional-deficient strain MAU1 (CCFM501) is taken as a material, an agrobacterium tumefaciens-mediated genetic manipulation technology is applied to obtain an omega 3 desaturase (omega 3Des) overexpression strain of high-yield EPA (eicosapentaenoic acid), and the strain and the construction method have important significance on basic theoretical research and product development of mortierella alpina, M. alpine as an oil-producing fungus.

The invention relates to a recombinant Mortierella alpine strain GPD-1 overexpressing G3PD1 and a construction method. According to the invention, uracil-auxotrophic Mortierella alpine MAU1 is used as a material, Agrobacterium tumefaciens is used for mediation, and a G3PD1-overexpressing recombinant strain with obviously increased lipid content is obtained. Compared with wild Mortierella alpine, the total lipid content of the strain provided in the invention is increased by about 50%, and the transcription amount of G3PD1 is increased by about 2 times; so theoretical and application foundations are laid for subsequent industrial application.

The invention discloses a method for fermentation production of arachidonic acid by adopting two-stage pH control. According to the method, mortierella alpine is taken as a production strain; a two-stage pH control strategy is adopted; the pH of a fermentation solution is regulated to be 4 to 6 in an early stage of fermentation; after the strain is transformed from a growth type to a grease accumulation type, the pH of the fermentation solution is regulated to 5 to 8 for fermentation culture. By adopting the method disclosed by the invention, the yield of the arachidonic acid can be remarkably increased.

The invention relates to a method for culturing cordyceps sinensis by utilizing astragalus membranaceus and an application thereof. The method comprises the following steps of: in the culture and / or fermentation process of cordyceps sinensis seeds, adding the astragalus membranaceus into a culture medium of the cordyceps sinensis, and continuously culturing and fermenting according to the conventional process of the cordyceps sinensis, wherein the preferred cordyceps sinensis is anamorph Chinese mortierella, teleomorph Chinese mortierella, cephalosporium sinensis, tolypocladium sinense, synnema or paecilomyces hepiali. The method provided by the invention is simple and convenient, the needed astragalus membranaceus extract is less and the cost is low; and the strain is fast in growth and excellent in quality, and the obtained fermentation liquid and mycelia are high in cordycepin content. The fermentation liquid and mycelia cultured by the method can be used for producing cordyceps sinensis health-care products.

The invention discloses a mortierella alpine mutant strain, a method for producing arachidonic acid oil by using the same and arachidonic acid oil. The mortierella alpine mutant strain (Mortierella alpine Y16) is preserved in the China Center for Type Culture Collection (CCTCC) on July 2, 2015, the preservation address is Wuhan University in Wuhan City of China, and the preservation number is CCTCC NO: M2015421. In the arachidonic acid oil produced by using the mortierella alpine mutant strain, the content of triglyceride is more than or equal to 92wt%, the content of arachidonic acid is at least 48wt%, and the content of long-chain saturated fatty acid with more than 20 carbons is lower than 6.3wt%.

The invention belongs to biotechnology field and particularly relates to high throughput screening of an effective anti-hepatic fibrosis compound by means of picric acid-sirius red staining method on a collagenous fiber. The cell is hepatic stellate cell line CFSC-8B, transforming growth factor TGF-beta1 with the dose of 10ng / mL can obviously induce the CFSC-8B cell to secrete collagen when acting for 48 hours, and the secretion of the CFSC-8B cell collagen induced by the TGF-beta 1 can be effectively detected by means of picric acid-sirius red staining method. The picric acid-sirius red staining method on the collagenous fiber is applied to screening the compounds with anti-hepatic fibrosis activities in different polarity extracts of antrodia camphorata, ganoderma lucidum, cephalosporium sinensis, cordyceps mortierella, armillaria and hericium erinaceus powder. Tests prove that n-hexane and chloroform extract of the antrodia camphorata, ethyl acetate extract from the cordyceps mortierella, and n-hexane, chloroform extract and ethyl acetate extract from the armillaria can effectively inhibit the secretion of the CFSC-8B cell collagen induced by the TGF-beta 1, the above extracts have dose dependent relationship, are significant in difference, and have an anti-hepatic fibrosis function, and other extracts do not have significant inhibition effect.