59 results about "Hyphantria" patented technology

The invention discloses a simple stereoselective synthesis method of sex pheromones of hyphantria cunea, which relates to epoxypropane compounds. The sex pheromones of hyphantria cunea III[(9S,10R)-9,10-epoxy-(3Z,6Z)-3,6-heneicosenediene] and IV[(9S,10R)-9,10-epoxy-(3Z,6Z)-3,6-heneicosenetriene] are prepared from cheap and readily available 2-propargyl alcohol serving as raw materials by eight steps with high efficiency, high enantioselectivity and high yield, wherein the total yield of (9S,10R)-III is 36 percent; the total yield of (9S,10R)-IV is 33 percent; and enantiomer excess e is more than 99 percent. The method has the advantages that: the operation and separation of each step are simple; the yield is high; all the adopted reagents are common reagents which are cheap and readily available; and the production line is short. The method is suitable for industrial production.

The present invention discloses a plurality of indolocarbazole alkaloids having strong insect disinfestation activities, and a production strain of the indolocarbazole alkaloids. The indolocarbazole alkaloid compound production strain CQT14-24 is a marine Streptomyces nitrosporeus CQT14-24 strain, wherein the strain is subjected to artificial culture, and the obtained fermentation product is extracted by the conventional chemical method so as to obtain four indolocarbazole alkaloids and an anthraquinone compound. According to the present invention, the indolocarbazole alkaloids and the one anthraquinone compound provide strong killing effects on a variety of cultivated plant pests, particularly the pests having characteristics of spreading in agriculture and forestry production and serious harm and comprising Pieris rapae, Brevicoryne brassicae, Hyphantria cunea, Plutella xyllostella, Mythimna separate and Helicoverpa armigera, wherein the relative mortality rate achieves 81.9-100%, the polypide is seriously reduced, the growth inhibition rate is 100%, and the imago pupation rate is reduced by 70.4-86.7%; and the strain is subjected to acclimation through the artificial culture, and has characteristics of stable feature, easy culture, simple and feasible operation method, no environmental pollution, short period, cheap raw materials, low production cost, good development prospect, and easy industrialization achieving.

The invention discloses isaria javanica for preventing and treating hyphantria cunea,wherein the isaria javanica is classified and named as isaria javanica BE01, which is preserved in China General Microbiological Culture Collection Center in No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing on November 28, 2016 with preservation number of CGMCC No.13192. The isaria javanica BE01 strain provided by the invention is separated from stiff hyphantria cunea larvae. Tests prove that the isaria javanica, under a spore concentration higher than 1.0*10<4> spores / mL, has relatively good virulence on the hyphantria cunea at different ages, and an infection rate exceeds 53.33%, and the infection rate of the isaria javanica, under a spore concentration of 1.0*10<8> spores / mL, exceeds 73.33%; therefore, the isaria javanica is capable of effectively preventing and treating the hyphantria cunea.

The invention discloses an artificial multiplication method for tetrastichus septentrionalis. The method includes the following steps of (1) selection of a substitute host; (2), field collection and selection of micromelalopha troglodyta pupa; (3), cultivation of bee species; (4) extraction of information matter; (5), processing of antheraea pernyi pupa; (6), bee receiving; (7), cultivation and development. According to the artificial multiplication method, pupa which is not subjected to parasitism is soaked with ethyl alcohol, pupa surface extract is obtained, then the pupa surface extract is sprayed on the antheraea pernyi pupa in an artificial spraying mode, the tetrastichus septentrionalis is induced to be subjected to parasitism, and the parasitism rate is raised. The tetrastichus septentrionalis is subjected to indoor large-scale multiplication, and a practical technical means is provided for preventing and controlling hyphantria cunea, micromelalopha troglodyta and other lepidoptera pests for forest creatures.

The invention provides a Hyphantria cunea Drury attractant composition, its application and attracting core. The Hyphantria cunea Drury attractant composition includes Hyphantria cunea Drury sex pheromone and its synergistic agent. The synergistic agent contains one or more of the following plant source smell components: beta-ocimene, hexanal, cis-3-hexenal, diacetone alcohol, diisopropylcarbinol, 6-methyl-5-heptene-2-one, trans-3-hexenol, o-xylene, hemimellitene, trans-2-hexenal, and limonene. The attractant composition provided in the invention can be prepared into an attracting core so as to be used cooperatively with the existing Hyphantria cunea Drury sex pheromone. The invention provides the attracting core containing the Hyphantria cunea Drury attractant composition to prevent, control and detect Hyphantria cunea Drury, so that the defects of high cost and difficult popularization of imported and domestic Hyphantria cunea Drury sex pheromone attracting cores in the prior art are solved. The attracting core provided in the invention has the advantages of low cost, flexible use, and higher trapping efficiency.

The invention discloses isaria farinose HS05 and application. The classification of isaria farinose is named as Isaria farinose HS05, the strain is preserved in China General Microbiological Culture Collection Center on May, 9, 2016, the preservation number is CGMCC No.12247, and the preservation address is No.3, Courtyard No.1, Beichen West Road, Chaoyang District, Beijing. The isaria farinose HS05 is high in sporulation capacity and high in insecticidal activity on larvae of aphrodisium sauteri matsushita, and the fatality rate is larger than 80%. By means of the isaria farinose HS05, the population quantity of the aphrodisium sauteri matsushita can be effectively controlled. In addition, the isaria farinose has the excellent control effect on other forest pests such as dendrolimus spp., monochamus alternatus hope and hyphantria cunea, and very good application and popularization value is achieved.

The invention discloses a synthetic method of sex pheromone intermediate (2S,3R)-2,3-epoxy-1-tetradecanol (4) of Hyphantria cunea. According to the method, firstly, nickel acetate tetrahydrate, sodiumborohydride, ethylenediamine as well as an alcohol as a solvent are added to a hydrogenation reactor for a reaction, then, an alcoholic solution with 2-tetradecynol dissolved is added continuously, areaction is performed after hydrogen replacement, and (Z)-2-tetradecene-1-ol is obtained; (Z)-2-tetradecene-1-ol is dissolved in a halogenated hydrocarbon solvent, a carbonate solution and a halogenated hydrocarbon solution of 3-chloroperoxybenzoic acid are added for a reaction, and (2S,3R)-2,3-epoxy-1-tetradecanol is obtained. The method is environmentally friendly and suitable for industrial production, cost is low, and reaction conditions are mild.

The invention discloses a hyphantria cunea tachykinin receptor gene, dsRNA and application in preventing and treating hyphantria cunea. The tachykinin receptor gene has a nucleotide sequence shown asSEQ ID No.1 and encodes a protein having an amino acid sequence shown as SEQ ID No. 2. The tachykinin receptor gene dsRNA has a nucleotide sequence shown as SEQ ID No. 3. The hyphantria cunea tachykinin receptor gene dsRNA is applied to the prevention and treatment or preparation of products for preventing and treating hyphantria cunea. The hyphantria cunea tachykinin receptor gene and dsRNA can reduce the intake of hyphantria cunea larvae, significantly reduce the hunger resistance of larvae, weaken the vitality of hyphantria cunea, and finally achieve the prevention and treatment effect.

The invention provides a hyphantria cunea viral insecticide and a preparation method thereof. The preparation method comprises the following steps: feeding hyphantria cunea by virtue of artificial feed, preparing a nuclear polyhedrosis virus precipitate, and adding trace inorganic nano-materials, aromatic amino acids and a small amount of refined fluorescent whitening agent into the virus precipitate. According to the preparation method, the viral insecticide is complete in components and high in suspension property, the quality guarantee period is prolonged, the insecticide has a good protective effect against ultraviolet light during field application, the virus duration is long, and the control effect is greatly improved.

The invention discloses an artificial propagation method of Coccygomimus disparis, comprising the following steps of a, choosing a fresh pupa of hyphantria cunea as a host, half embedding the host into wet sandy soil and putting in a raising gauze cage; b, pairing male and female adults of the Coccygomimus disparis and leading in the raising gauze cage to ensure that the adults freely mate under the conditions of room temperature and natural light, spawn and parasitically live in the host; and c, taking out the hose, culturing the host under the conditions that the temperature is 21-28 DEG C and the relative humidity is 50-70% and obtaining next-generation Coccygomimus disparis through eclosion. The method is not limited by season and capable of massively propagating the Coccygomimus disparis indoors, therefore, long-term massive supply source of the Coccygomimus disparis is guaranteed and long-term effective biological control can be carried out on lepidopterous defoliators such as woodland gypsy moth, the hyphantria cunea, pine moth and the like.

The invention discloses a synthetic method of hyphantria cunea sex pheromone components, and belongs to the technical field of chemical production. (2S, 3R)-2, 3-epoxy-1-tetradecanol is used as a rawmaterial, and the hyphantria cunea sex pheromone components (3Z, 6Z, 9S, 10R)-9, 10-epoxy-3, 6-heneicosadiene and (3Z, 6Z, 9S, 10R)-9, 10-epoxy-1, 3, 6-heneicosatriene are obtained through bromination, coupling and hydrogenation. The process is mild and controllable in reaction, good in yield, simple and convenient to operate, few in reaction steps, low in production cost and suitable for large-scale production. The lure prepared from the sex pheromone components synthesized by the synthesis method can effectively trap and kill a large number of hyphantria cunea male moths in combination witha trap, is applied to hyphantria cunea pest situation forecasting and green prevention and control, and can reduce chemical pesticide application, thereby reducing pollution, protecting the environment and remarkably improving economic, social and ecological comprehensive benefits.

The invention relates to streptomycete and application thereof. The strain is Streptomyces regensis and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 16746. The streptomycete can be used in the field of biological prevention and control and is used for preventing and controlling camptoloma interiorata, hyphantria cunea, plutella xylostella, ostriniafurnacalis, tetranychus urticae, cabbage caterpillar, mamestra brassicae, henosepilachna vigintioctomaculata, myzus persicae and dolycoris baccarum Linnaeus. The streptomycete has the characteristicsof high insecticidal activity, wide insecticidal spectrum, good genetic stability and the like; a strain fermentation solution has the advantages of strong temperature tolerance, stable insecticidalstability, good light stability, storage resistance and the like, and has very good development and application values.

The invention discloses a vertical multi-layer closed nuclear polyhedrosis virus multiplication process, which is accomplished by the following steps: 1, selecting a land, which has an area of more than or equal to 50m<2> and has naturally or artificially growth plant feed around and a water source nearby, as a multiplication room; 2, building a vertical trapezoid platform in the multiplication room for breeding larvae; 3, closing the doors and windows of the multiplication room; 4, collecting 2-2.5-year-old larvae of hyphantria cunea and placing the larvae on the multiplication platform flatly; 5, spraying viral liquid onto the placed larvae of hyphantria cunea; and 6, collecting corpses of larvae dead of diseases in a container containing sterile water to soak the corpses for 2 to 4 days, grinding and crushing the corpses in a homogenizing crusher to obtain homogenate, filtering the homogenate with a gauze element, subjecting residue filtered out to secondary grinding, filtering with the gauze element, and obtaining hyphantria cunea polyhedrosis virus liquid. The process technique is simple, can improve the number of multiplied viruses, reduce the cost of virus multiplication, facilities the collection of dead larvae infected with the virus and is suitable to be promoted and used in vast rural areas.

The invention discloses a constant temperature detection kit for Hyphantria cunea nucleic acid and a detection method thereof. The kit includes: 1) a DNA detection device; 2) a Hyphantria cunea nucleic acid constant temperature amplification reaction solution; 3) a positive control, which is a DNA fragment containing a Hyphantria cunea mitochondrion COI gene; and 4) a negative control, which is sterile double distilled water. The detection kit provided by the invention has the characteristics of high specificity, high sensitivity and fast reaction speed. From sample treatment to the end of detection on a single sample, only 1-1.5 hours are needed. The kit can meet middle flux and low flux sample detection, is especially suitable for field detection, and the whole reaction process only needs a constant temperature instrument.

The invention belongs to the technical field of agriculture, and discloses a comprehensive control method for hyphantria cunea. The control method comprises the following steps that (1) in the larvalphase and the pupating early stage of hyphantria cunea, hyphantria Chouioia cunea Yang are released continuously for prevention and control for two generations; (2) in the low-age larvae period, a larvae net curtain and harmful branches are pruned, the cut branches and net curtain are removed from an orchard to be burnt down in a concentrated mode; plant insecticides are sprayed to the low-age larva; and (3) before the old aged larva begin to leave a tree for pupating, a place of 1-1.5 meters from the ground of the trunk is bundled by grass for one circle for trapping aged larva to pupate in aconcentrated manner; and (4) hyphantria cunea adults are trapped by a trapper, wherein the trapper is provided with a trapping agent composition, and hyphantria cunea are cleared from the trapper intime. According to the method, different insecticidal means are adopted according to different growth periods of the hyphantria cunea, and artificial physical control, biological control and chemicalcontrol combined means are adopted, so that the insect mouth cardinality and propagation diffusion of the hyphantria cunea are thoroughly controlled.

The invention discloses a hyphantria cunea sex attractant containing a synergist. The sex attractant contains the following components: 85%-95% of hyphantria cunea sex pheromone and 5%-15% of a synergist, wherein the synergist is a long-carbon chain olefin substance. Compared with an existing hyphantria cunea sex attractant, the moth attraction effect of the high-efficiency hyphantria cunea sex attractant disclosed by the invention is obviously enhanced.

The invention discloses a control system of a spraying robot for a hyphantria cunea larva net curtain. The control system comprises an industrial control computer unit and is characterized in that the industrial control computer unit is connected with a four-axis driver unit, an electric spray head unit and an industrial camera unit, and the four-axis driver unit is connected with a step motor drive unit and a micro-switch limit detection unit. The industrial control computer unit sends control signals to the four-axis driver unit, industrial camera unit and electric spray head unit, the four-axis driver unit controls the step motor drive unit to work according to the received control signals, the micro-switch limit detection unit acquires the position information of a step motor and sends signals to the four-axis driver unit, and the four-axis driver unit controls the work or stop of the step motor drive unit according to the received signals. The control system of the spraying robot for the hyphantria cunea larva net curtain realizes the automatic spraying of the intelligent target spraying robot, improves the spraying efficiency and lowers the pesticide pollution.

The invention belongs to the technical field of pesticide, in particular to application of osthole for preparing a preparation for killing hyphantria cunea drury. The invention provides a preparation which contains 0.0001 percent-99.9999 percent of osthole and 99.9999 percent-0.0001 percent of excipient and a method which does not need an organic solvent when the preparation is prepared and has good water dispersibility, high chemical stability and convenient storage and transportation. The prepared preparation has more favorable touch killing effect on the hyphantria cunea drury and is safe to people, livestock and other mammals. Compared with common chemical pesticides, the invention is more beneficial to the environmental protection, has the characteristics of safety, high efficiency, short residual period, no poison, no pollution and convenient use, storage and transportation, and is novel hyphantria cunea drury insecticide.

The invention discloses Hyphantria cunea Iap gene dsRNA and a bacterial expression liquid thereof. The nucleotide sequence of the Iap gene dsRNA fragment is shown in SEQ ID NO. 2. According to the invention, a large amount of required exogenous target dsRNA is expressed in Escherichia coli HT115 by using recombinant plasmid; after the Escherichia coli HT115 bacterial liquid for IPTG induced expression of the target dsRNA is continuously fed, the bacterial expression liquid has high lethal ability to Hyphantria cuneas and significant inhibitory effect on the growth and development of the Hyphantria cuneas; and a method for preventing and treating the Hyphantria cuneas by using the bacterial expression liquid provided by the invention has the advantages of strong practicability, convenient operation, good effectiveness and sensitivity, high insecticidal efficiency, environmental friendliness, etc., and has good application prospects.

The invention discloses weeding granule used for paddy field and applicable to prevention and treatment of Hyphantria cunea through aerial application of pesticides. The weeding granule is characterized by comprising 0.2%-20% of effective ingredients, 0.1%-0.5% of a wetting agent, 1%-5% of a dispersing agent, 0.5%-5% of an emulsifying agent, 0.5%-2% of a binding agent, 0.1%-0.5% of a warning color, with the balance being a carrier, wherein the total content of active components accounts for 0.2-20% of the granule. The granule is characterized in that the diameter of the granule is in a range of 1-4 mm, the specific gravity of the granule is 0.9-1.5 g / cm<3>, and the granule has an anti-drifting effect, and can be applied by an unmanned aerial vehicle after transplanting, thereby reducing labor intensity and improving working efficiency.

The invention provides a method for preventing and treating major diseases of walnut trees, and relates to the technical field of fruit tree plant disease and insect pest prevention and treatment. The method is characterized by mainly comprising the steps including walnut heliodinidae prevention and treatment, hyphantria cunea prevention and treatment and scarab prevention and treatment. The method provided by the invention has the advantages that the method is reasonable, the implementation is convenient, and the effect is good.

The invention discloses a method for quickly detecting hyphantria cunea. The method comprises the following steps of preparing DNA, carrying out a PCR reaction system and reaction conditions, and judging PCR results. The method for quickly detecting the hyphantria cunea is an efficient, accurate and convenient hyphantria cunea molecule detection technology, and has the advantages of capability of distinguishing the hyphantria cunea and other species of arctiidae at a molecule level, capability of meeting the usage requirement and good practicability, so that good economic benefits and social effects can be generated.

The invention relates to a method for determining a remote sensing optimal diagnosis time period of the harm of hyphantria cunea. The method comprises the following steps of (1) determining a diagnosis threshold of the harm of the hyphantria cunea based on measured data and a mutation theory; (2) obtaining a multi-time-point remote sensing image based on the available multi-time-point remote sensing image; and obtaining the remote sensing response characteristics including the leaf area index (LAI), the characteristic spectrum index (CSI), the normalized difference mountain vegetation index (NDMVI), the global vegetation humidity index (GVMI) and the like of bamboo poison moth hazard detection respectively, establishing the time sequence data of all the indexes according to the characteristics, and extracting the bamboo poison moth hazard level; and (3) counting insect pest risk evaluation index values, drawing a curve graph, and respectively determining the optimal monitoring and early warning time period of the insect pests. The method can provide an important basis for remote sensing detection and accurate prevention and control of the hyphantria cunea insect pests.

The invention discloses a hyphantria cunea cecropin A gene as well as expression protein and application thereof. The hyphantria cunea cecropin A gene has a DNA (deoxyribonucleic acid) sequence as shown in SEQ ID NO.1 and an amino acid sequence as shown in SEQ ID NO.2. According to the hyphantria cunea cecropin A gene, the full-length cDNA (complementary DNA) sequence and the expression protein of the cecropin A gene cloned from hyphantria cunea for the first time are obtained, hyphantria cunea cecropin A antibacterial peptide with amidated C end is synthesized for the first time by a chemical method according to the protein sequence, and the antibacterial activity of synthetic hyphantria cunea cecropin A antibacterial peptide on escherichia coli K12D31 (E.coli K12D31) and agrobacterium EHA105 is tested by an agarose hole diffusion method; the hyphantria cunea cecropin A gene is very high in antibacterial activity and has a wide application prospect in bacterial inhibition.

The invention relates to an insecticidal composition containing a methoprene compound, and application of the insecticidal composition. The composition comprises an active component A and an active component B, wherein the active component A is one selected from (S)-methoprene and methoprene, the active component B is one selected from indoxacarb, metaflumizone, spinosad, spinetoram and chlorfenapyr, and the weight ratio of the active component A to the active component B is 80:1-1:80. According to the invention, the action mechanisms of the two active components in the composition are greatlydifferent, the composition has an obvious synergistic effect on mosquitoes, hyphantria cunea, locusts, solenopsis invicta and other pests after being compounded, and the production and application value is high.

The invention discloses a biological control method for hyphantria cunea. The method comprises the following steps: in a low-age larva stage of the hyphantria cunea, cutting off a larva net curtain and harmful branches, bagging and sealing the cut branches and the net curtain, and taking the branches and the net curtain away from a garden for centralized burning; in a hyphantria cunea mature larva stage, before hyphantria cunea mature larvas begin to get out of a tree and pupate, tightly binding the trunk with hay at a position 0.8-1.8 m away from the ground for a circle, trapping the hyphantria cunea mature larvas to pupate in the hay, replacing hay bundles every 5-7 days during pupation, bagging and sealing the replaced hay bundles, and taking the replaced hay bundles away from the garden for centralized burning; and in the initial stage of hyphantria cunea pupation, releasing parasitic wasps, and continuously releasing the parasitic wasps for control twice. The biological control method for the hyphantria cunea is simple, the means of combining artificial physical control and biological control is adopted, occurrence and propagation of the hyphantria cunea are controlled, the population base number and propagation and diffusion of the hyphantria cunea are thoroughly controlled, the use amount of chemical pesticide is reduced, and the yield and quality of agricultural products are improved.

The invention discloses an infrared sensing-based automatic-counting trap of adult hyphantria cunea and an application method of the infrared sensing-based automatic-counting trap. The infrared sensing-based automatic-counting trap of adult hyphantria cunea comprises a hanging handle, a cover cap, an upper cap of a cavity structure, a pest collecting bottle, a lure storage box, a power supply device and an automatic counting device, wherein the automatic counting device is fixedly installed in the upper cover, the upper cover is located at the opening of the top of the pest collecting bottle,and the upper cover is connected with the pest collecting bottle in a sealed mode, and the infrared sensing-based automatic-counting trap is characterized in that a drop hole is formed in the center of the top of the upper cover in a penetrating mode, and the lure storage box is installed at the drop hole of the upper cover. Through the infrared sensing-based automatic-counting trap, the number and time distribution of the adult hyphantria cunea which is trapped in a certain area can be recorded in real time and accurately, so that the activity rules of the local adult hyphantria cunea are understood more conveniently, and high cost which is caused by laborious manual investigation is reduced.

The invention discloses fluorescent quantitative reference genes of hyphantria cunea adults with different sexes as well as primers and application of the fluorescent quantitative reference genes, and belongs to the field of molecular biology of forestry insects. The fluorescent quantitative reference genes are RPL13 and RPS15 genes, and the nucleotide sequences of the fluorescent quantitative reference genes are shown as SEQ ID NO. 1 and SEQ ID NO. 2. According to a transcriptome sequencing database of hyphantria cunea, eight candidate reference genes commonly used as reference genes are selected, the stability of the candidate reference genes is evaluated through five algorithms, the fluorescent quantitative reference genes of hyphantria cunea adults with different sexes are obtained, the real-time fluorescent quantitative PCR primers of the reference genes are designed, and the primers are high in specificity, and high in amplification efficiency. The current situation that the hyphantria cunea adults with different sexes have no suitable reference genes is filled up, and a reliable analysis basis is provided for expression quantification of related functional genes of the hyphantria cunea under different sex experiment conditions.

The invention discloses new use of a terpene compound (3-carene), and relates to application of the terpene compound in biological prevention and control of hyphantria cunea. It is proved through experiments that the 3-carene which is a main component of monoterpene in essential oil has a repellent effect on the hyphantria cunea and can be combined with olfactory binding proteins CcOBP2 to triggeran olfactory reaction so that natural enemy insects chouioia cunea Yang can be attracted. Through the application, research on the behavioral ecology of the hyphantria cunea and the natural enemies chouioia cunea Yang thereof plays an important role in the biological prevention and control of the hyphantria cunea, and the application prospects are broad.