15 results about "Hyphantria" patented technology

The invention discloses a pheromone binding protein derived peptide capable of effectively monitoring sex pheromones of fall webworms and a biosensor, and belongs to the field of biosensors. At present, methods such as sample plot survey and sex traps commonly used in fall webworm monitoring have the problems of poor timeliness, high labor cost, insufficient data continuity and the like. In order to solve the problem, the pheromone binding protein-derived peptide provided by the invention has an amino acid sequence as shown in SEQ ID NO: 2, the biosensor comprises a substrate and an interdigital electrode, a single-walled carbon nanotube is attached to the electrode, and the electrode is also connected with the pheromone binding protein-derived peptide. The sensor can specifically detect sex pheromones of fall webworms, has no response to 17 plant volatile matters, and can detect sex pheromones released by as low as five live female fall webworms under laboratory conditions. The method has application potential in the fields of early pest monitoring, prevention and control efficiency improvement, ecological safety guarantee and the like.

The invention discloses a method for artificially breeding white moth gathering cotesia sinensis and applying the white moth gathering cotesia sinensis to biological control of fall webworm. According to the method, the key factors such as the optimal age, the optimal bee inoculation proportion and the optimal temperature of the hyphantria cunea larvae which are hosts for artificially breeding the cotesia cunea are determined, the parasitism rate and the offspring number of the cotesia cunea are effectively increased, the breeding period is shortened, and the bee breeding efficiency is improved. Meanwhile, an optimal scheme for prolonging the shelf life of the cotesia sinensis gather through low-temperature storage of the cocoons or newly-emerged adults is also defined. The new natural enemy product breeding application technology provided by the invention is specially applied to biological prevention and control of the fall webworms in the larval stage, not only enriches the types of existing fall webworm natural enemy products, but also provides a more comprehensive solution for biological prevention and control of the fall webworms, can reduce the excessive use of chemical pesticides, and is beneficial to environmental protection. And a powerful technical guarantee is provided for balance and health of an ecological system.

The invention belongs to the technical field of plant insect pest biocontrol, and discloses a hyphantria cunea biocontrol bacterium and application thereof, the hyphantria cunea biocontrol bacterium has a strain number of JSAFC 2084, is preserved in China General Microbiological Culture Collection Center (CGMCC), has a preservation time of September 23, 2024, has a preservation number of CGMCC No.41563, and is classified and named Saksenaea sp. The hyphantria cunea biocontrol bacterium strain provided by the invention can obviously inhibit the survival activity of hyphantria cunea, the death rate of hyphantria cunea larvae can reach 100% after 7 days of treatment, and the hyphantria cunea biocontrol bacterium strain can be used for preventing and controlling hyphantria cunea, is typical biological prevention and control, can avoid the problems of drug resistance, environmental pollution and the like caused by chemical prevention and control, and has a relatively good application prospect.

The invention discloses a freshwater mucor and application thereof, the strain number of the freshwater mucor is JSAFC 2076, the freshwater mucor is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 23, 2024, and the preservation number is CGMCC No.41523. The invention further discloses a preparation method of the freshwater mucor. The strain provided by the invention is a biocontrol fungus which can be used for preventing and treating major insect hyphantria cunea, has the characteristics of strong pathogenicity to hyphantria cunea larvae, safety to the environment and difficulty in causing drug resistance of insects, and can be applied to biological prevention and treatment of hyphantria cunea.

The application discloses an information pheromone binding protein derivative peptide and a biosensor capable of effectively monitoring the sex pheromone of Hyphantria cunea, and belongs to the field of biosensors. At present, the monitoring of H. cunea is usually achieved by means of sample plot investigation, sex lures and other means, and problems such as poor timeliness, high labor cost and insufficient data continuity exist. In order to solve the above problems, the information pheromone binding protein derivative peptide provided by the application has an amino acid sequence as shown in SEQ ID NO: 2, the biosensor comprises a substrate and an interdigital electrode, single-walled carbon nanotubes are attached to the electrode, and the information pheromone binding protein derivative peptide is further connected. The sensor can specifically detect the sex pheromone of H. cunea and has no response to 17 kinds of plant volatiles, and can detect the sex pheromone released by as low as five live female H. cunea under laboratory conditions. The sensor has application potential in the fields of early-stage pest monitoring, improvement of prevention and control efficiency and guarantee of ecological safety.

The application provides a screening method for effectors of foreign invasive species Hyphantria cunea larvae saliva and female insect oviposition secretions. The application collects saliva of invasive species Hyphantria cunea larvae and concentrates female insect oviposition secretions, then performs mass spectrometry analysis, performs KEGG and GO cluster analysis on the obtained protein information, and screens possible key proteins. Further, CRISPR / Cas9 and RNAi means are used to explore the functions and roles of these effectors in key damage-causing processes such as Hyphantria cunea larvae feeding and female insect oviposition, effectively realizing the screening of effectors of the foreign invasive pest, and providing effective means for preventing the maintenance and outbreak of Hyphantria cunea population and the damage of the population to agricultural and forestry crops.

The application relates to the field of molecular biological technology, and discloses dsRNA of a Hyphantria cunea gene and application thereof.The dsRNA of the Hyphantria cunea gene comprises at least one of LKB1 gene dsRNA, Foxo3 gene dsRNA and ECR gene dsRNA;the nucleotide sequence of the LKB1 gene dsRNA is shown as SEQ ID NO.1;the nucleotide sequence of the Foxo3 gene dsRNA is shown as SEQ ID NO.2; and the nucleotide sequence of the ECR gene dsRNA is shown as SEQ ID NO.3.Through the technical scheme, the problem of insufficient prevention and treatment of the Hyphantria cunea in the related art is solved, and the blank of screening of efficient target genes of a core channel of energy metabolism and development regulation of the Hyphantria cunea in the field of RNA interference prevention and treatment is filled.

The invention discloses an odor receptor derived peptide for effectively monitoring sex pheromones of spodoptera frugiperda and a biosensor, and belongs to the field of biosensors. At present, methods such as sample plot survey and sex traps commonly used in fall webworm monitoring have the problems of poor timeliness, high labor cost, insufficient data continuity and the like. In order to solve the problem, the pheromone binding protein-derived peptide provided by the invention has an amino acid sequence as shown in SEQ ID NO: 1, the biosensor comprises a substrate and an interdigital electrode, a single-walled carbon nanotube is attached to the electrode, and the electrode is also connected with the pheromone binding protein-derived peptide. The sensor can specifically detect sex pheromones of fall webworms, and has no response to 17 plant volatile matters. The method has application potential in the fields of early pest monitoring, prevention and control efficiency improvement, ecological safety guarantee and the like.

The present invention discloses a Lunasporangiospora chienii and its application. The strain number of the Lunasporangiospora chienii is JSAFC 2261, which was deposited at the General Microbiological Center of the China Committee for Culture Collection of Microorganisms on September 23, 2024, and the deposit number is CGMCC No. 41524. The Lunasporangiospora chienii strain provided in this application has strong pathogenicity to the larvae of Hyphantria cunea and Anomala corpulenta, is environmentally friendly and pollution-free, and is not prone to drug resistance. It can be used as a live biological pesticide and has very strong application potential in the biological control of Hyphantria cunea and / or Anomala corpulenta.

The invention discloses a pheromone binding protein derived peptide for effectively detecting sex pheromones of fall webworms and a biosensor, and belongs to the field of biosensors. At present, methods such as sample plot survey and sex traps commonly used in fall webworm monitoring have the problems of poor timeliness, high labor cost, insufficient data continuity and the like. In order to solve the problem, the pheromone binding protein-derived peptide provided by the invention has an amino acid sequence as shown in SEQ ID NO: 2, the biosensor comprises a substrate and an interdigital electrode, a single-walled carbon nanotube is attached to the electrode, and the electrode is also connected with the pheromone binding protein-derived peptide. The sensor can specifically detect sex pheromones of fall webworms, and has no response to 17 plant volatile matters. The method has application potential in the fields of early pest monitoring, prevention and control efficiency improvement, ecological safety guarantee and the like.

The invention belongs to the technical field of insect-resistant research, and particularly relates to application of a small-molecule compound SPD to resistance of hyphantria cunea pests of poplars. Comprising the following steps: (1) taking a plurality of populus tomentosa plant seedlings growing for 3 months in a greenhouse; (2) adding water into the spermidine solution in proportion, and respectively diluting the spermidine solution into working concentrations of 0.5 mM, 1.0 mM, 2.0 mM and 4.0 mM; (3) taking the diluted spermidine solution as a treatment group and an aqueous solution group or a control group, respectively spraying the leaves of the poplar twice a day, continuously spraying for 3 days at the interval of 6 hours, and then culturing in a greenhouse; and (4) 36 hours after the last time of spraying, taking the seventh leaf and the contrast, and carrying out a hyphantria cunea feeding experiment. According to the method, a new green protection strategy is provided for the artificial forest to cope with the fall webworm pests, pesticide dependence is reduced, and agriculture and forestry and sustainable development are promoted.

The application discloses a method and system for realizing all-year reproduction of Hyphantria cunea by using an intelligent artificial climate chamber, and belongs to the technical field of artificial feeding and breeding of insects. In the intelligent artificial climate chamber with programmable segmented control functions of temperature, relative humidity and illumination, a day / night two-segment cycle control program is continuously run, long illumination and suitable temperature are provided in the day segment, illumination is turned off and temperature is reduced in the night segment, constant humidity and timed fresh air ventilation are maintained throughout the process, and a stable development environment for the Hyphantria cunea is provided throughout the year. The all-year reproduction system of the Hyphantria cunea comprises an intelligent artificial climate chamber, a mating and egg-laying unit, an egg hatching unit, a larva feeding unit, a pupa induction unit and a transfer unit. The method can be used to stably obtain a continuous source of the Hyphantria cunea throughout the year, reduce the influence of seasonal interruption of the insect source on experiments, and improve the repeatability and standardization level of the feeding process.

The present invention relates to the technical field of biological control, and discloses a biological control fungus for Hyphantria cunea and its application. The biological control fungus is taxonomically named Entomortierella chlamydospora, deposited in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms on September 23, 2024, with the deposit number CGMCC 41564. The biological control fungus strain for Hyphantria cunea provided in this application can significantly inhibit the survival activity of Hyphantria cunea. After 7 days of treatment, the mortality rate of Hyphantria cunea larvae can be increased to 96.7%, and the pupae can die during the pupal stage and cannot emerge. It can be used to control Hyphantria cunea, which is a typical biological control method, and can avoid problems such as drug resistance and environmental pollution caused by chemical control, and has good application prospects.

The invention is applicable to the field of microbial pesticides, and provides serratia marcescens and application thereof in prevention and treatment of fall webworm, the serratia marcescens is YZNUH-02, and the preservation number is CGMCC No.36002. The serratia marcescens YZNUH-02 strain provided by the invention is separated from naturally infected fall webworm larvae, and the serratia marcescens YZNUH-02 strain can be applied to prevention and treatment of fall webworm. The composition has strong pathogenicity on hyphantria cunea larvae, has high insecticidal efficiency, takes effect more quickly compared with the existing biological control means, and can quickly control the population quantity of hyphantria cunea. The strain and the related biopesticide belong to microbial source preparations, are green and environment-friendly, cannot cause environmental pollution after being used, cannot cause drug resistance of pests, and accord with the sustainable prevention and control concept.