10 results about "Populus tomentosa" patented technology

This invention discloses a method for establishing a high-efficiency leaf regeneration system for aseptic seedlings of Populus tomentosa 'Bofeng No. 3', belonging to the field of agricultural biotechnology. This invention, through 5-AU combined with dark culture treatment, not only induces early leaf differentiation in aseptic seedlings of Populus tomentosa 'Bofeng No. 3', but also significantly increases the number of adventitious buds and the rooting rate. Through chemical treatment and optimized culture conditions, this invention successfully established a high-efficiency plant regeneration system for aseptic leaves of Populus tomentosa 'Bofeng No. 3', laying the foundation for the genetic transformation and gene editing of Populus tomentosa 'Bofeng No. 3', and also providing a reference for the establishment of tissue culture systems for other tree species.

The invention belongs to the technical field of gene engineering, and particularly relates to application of an expression promoter of a PubHLH47 gene to improvement of salt stress resistance of populus japonica, and the nucleotide sequence of the PubHLH47 gene is as shown in SEQ ID NO.1. The PubHLH47 gene provided by the invention is utilized to construct a plant expression vector, so that the PubHLH47 gene is over-expressed in the populus japonica, and the salt stress resistance of the populus japonica is improved. A PubHLH47 gene overexpressed plant has the characteristic of effectively improving the tolerance of the plant under the stress of high salt. The PubHLH47 gene capable of improving the salt stress resistance is found in forest for the first time, an excellent gene resource is provided for promoting forest genetic engineering breeding, and a scientific basis is provided for later improvement of populus nigra.

The invention relates to the technical field of forest tree breeding and cultivation, in particular to an integrated method for improved variety breeding, rapid propagation and container seedling cultivation of poplar pie suitable for semi-arid regions. The integration method comprises the following steps: hybridizing populus arabinosa or populus sinkiang as a female parent and populus tomentosa or populus arabinosa as a male parent, screening by using a molecular marker to obtain F1-generation plants with salt-tolerant and drought-resistant characteristics, and sequentially carrying out rapid propagation, container seedling cultivation and afforestation planting on the F1-generation plants. Compared with the prior art, the method can solve the problem of rooting of the poplar pie, the afforestation survival rate is remarkably increased, and the method has the advantages of shortening the breeding period and accurately improving the stress resistance.

The invention relates to the technical field of gene engineering, in particular to application of a populus tomentosa PtTCH4 gene in regulation and control of plant growth and development. The nucleotide sequence of the populus tomentosa PtTCH4 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the protein coded by the populus tomentosa PtTCH4 gene is as shown in SEQ ID NO.2. Through overexpression of the PtTCH4 gene, the inflorescence color of a transgenic plant is lighter than that of a wild plant, and meanwhile, the inflorescence is increased; the regulation of plant inflorescence development is of great significance to promotion of plant genetic improvement and breeding.

The invention relates to a group of SNP (Single Nucleotide Polymorphism) molecular markers associated with surface area characters of poplar roots and application, and belongs to the technical field of genetic breeding of poplar. The method comprises the following steps: identifying key sites influencing the root surface area through whole genome association analysis, and respectively positioning the key sites at the 10391007bp (SAsite1) and the 10391759bp (SAsite2) on the No.6 chromosome of a populus sibirica genome; the two sites are completely linked, and the root surface area of the heterozygous haplotype (GA / CG) is obviously larger than that of the homozygous haplotype (GG / CC). The molecular marker provided by the invention can be used for early molecular auxiliary selection of the surface area of the root of the poplar, is beneficial to accurately and efficiently screening a poplar clone with a relatively large root surface area, and provides reliable information for improving the survival rate of vegetative propagation and breeding an excellent strain.

The invention belongs to the technical field of biology, and particularly relates to application of a PtoHSFC1 gene in regulating and controlling cold resistance and heat resistance of a poplar, and the nucleotide sequence of the PtoHSFC1 gene is shown as SEQ ID NO.1. Through bioinformatics analysis, inducible expression analysis, target gene overexpression and temperature treatment of a knockout plant, the result shows that the heat resistance and cold resistance of the populus tomentosa can be improved by improving the expression level of the PtoHSFC1 gene.

This invention relates to the field of genetic breeding technology, and specifically discloses a type of Populus tomentosa. PdbRAP2 Genes and their applications, the aforementioned PdbRAP2 The CDS sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence it encodes is shown in SEQ ID NO.2. This invention utilizes genetic engineering to overexpress *Populus sanguisorbus*. PdbRAP2 Genes have improved the drought resistance of Populus tomentosa.

This invention provides a salt-tolerant Populus tomentosa RHB1A This invention relates to genes and their applications, belonging to the field of genetic engineering technology. The salt-tolerant gene from Populus tomentosa provided by this invention... RHB1A The nucleotide sequence is shown in SEQ ID NO.1, and the encoded amino acid sequence is shown in SEQ ID NO.2. This invention screened an E3 ubiquitin ligase RHB1A and cloned its full-length CDS sequence. This was then demonstrated by overexpression in Populus tomentosa plants. RHB1A The gene significantly improved the plant's salt tolerance, providing theoretical support for the breeding and widespread promotion of salt-tolerant tree varieties.

The invention belongs to the technical field of insect-resistant research, and particularly relates to application of a small-molecule compound SPD to resistance of hyphantria cunea pests of poplars. Comprising the following steps: (1) taking a plurality of populus tomentosa plant seedlings growing for 3 months in a greenhouse; (2) adding water into the spermidine solution in proportion, and respectively diluting the spermidine solution into working concentrations of 0.5 mM, 1.0 mM, 2.0 mM and 4.0 mM; (3) taking the diluted spermidine solution as a treatment group and an aqueous solution group or a control group, respectively spraying the leaves of the poplar twice a day, continuously spraying for 3 days at the interval of 6 hours, and then culturing in a greenhouse; and (4) 36 hours after the last time of spraying, taking the seventh leaf and the contrast, and carrying out a hyphantria cunea feeding experiment. According to the method, a new green protection strategy is provided for the artificial forest to cope with the fall webworm pests, pesticide dependence is reduced, and agriculture and forestry and sustainable development are promoted.

The invention discloses a poplar cold-resistant whole genome SNP site combination, a probe, a chip and application thereof, and relates to the technical field of molecular breeding. The invention provides a cold-resistant whole genome SNP (Single Nucleotide Polymorphism) site combination of a poplar. The cold-resistant whole genome SNP site combination comprises 40141 SNP sites. According to the method, 639 poplar re-sequencing materials are utilized, a plurality of algorithms are selected to correlate with the cold resistance related SNP markers, whole genome selection models of different data sets are constructed, and the prediction effect is good. The marker combination is distributed on a whole genome, can provide theoretical and data support for the cold-resistant breeding work of the poplar, has relatively important theoretical value and application significance, and effectively solves the problems that the traditional cold-resistant breeding of the poplar depends on manual selection, the workload is large and the efficiency is low.