38 results about "Populus tomentosa" patented technology

Provided is the use of overexpression of the auxin transporter PtoPIN1a gene in increasing the biomass of Populus tomentosa. The nucleotide sequence of the auxin transporter PtoPIN1a gene is as shown in SEQ ID No. 1. By means of using a genetic engineering method, wild-type Populus tomentosa is transformed by means of Agrobacterium tumefaciens infection to obtain a tissue-specific PtoPIN1a overexpressing transgenic plant, and subsequently, a plant with a relatively high expression level is selected, by means of positive identification, for phenotype observation. Results show that compared with the wild type in the same period, the PtoPIN1a transgenic plant has an increased plant height, a thicker stem and a significantly increased aboveground biomass. By means of the provided technical cultivation method, a high-biomass high-quality poplar variety is cultivated. The method is of great significance for future molecular breeding to cultivate excellent poplar varieties.

The invention provides application of a poplar PtoHsfB1 gene in plant growth, and belongs to the technical field of gene engineering. The function of promoting plant growth of the populus tomentosa gene PtoHsfB1 is found for the first time. Experimental results show that the expression quantity of the PtoHsfB1 gene in the transgenic populus tomentosa is greatly improved compared with that of non-transgenic populus tomentosa, the height, dry weight and fresh weight of a transgenic plant are higher than those of a non-transgenic plant, ABA synthesis and signal transduction can be reduced through overexpression of the gene PtoHsfB1, growth and development of the populus tomentosa are promoted, and the yield of the populus tomentosa is increased. Therefore, a new genetic resource is provided for improvement and molecular breeding of the poplar and other woody plants.

The invention relates to the technical field of plant genetic engineering, in particular to a PtoNAC90 gene for regulating formation of poplar wood and application of the PtoNAC90 gene. The nucleotide sequence of the PtoNAC90 gene is as shown in SEQ ID No. 1; the amino acid sequence of the PtoNAC90 gene is as shown in SEQ ID No. 2. According to the invention, a transgenic plant is obtained by overexpressing PtoNAC90 in 741 populus tomentosa, and compared with a wild type, the transgenic populus with overexpressed PtoNAC90 has the advantages that xylem thickness and lignin content are increased, which indicates that the PtoNAC90 gene has potential application value in improvement of populus wood yield, and wood fast growth and wood property new variety improvement can be regulated and controlled by using a genetic engineering technology.

The invention relates to the technical field of plant genetic engineering, in particular to an application of a poplar PtbHLH47 gene and an encoding protein thereof in improving the salt tolerance of plants. The invention discloses application of a PtbHLH47 gene to improvement of plant salt tolerance and improvement of chlorophyll content of plant leaves. The nucleotide sequence of the PtbHLH47 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the expression protein of the PtbHLH47 gene is as shown in SEQ ID NO. 2. PtbHLH47 genes are cloned from populus tomentosa leaves, overexpressed PtbHLH47 transgenic populus tomentosa is obtained through an agrobacterium tumefaciens-mediated populus genetic transformation system, salt tolerance evaluation shows that the salt tolerance of the overexpressed PtbHLH47 transgenic populus tomentosa is remarkably improved, particularly, the chlorophyll content in the populus tomentosa leaves under salt stress can be remarkably improved, and the chlorophyll content in the populus tomentosa leaves under salt stress can be remarkably reduced. And gene resources are provided for cultivating salt-tolerant plants.

The invention discloses an application and a method of an overexpression transcription factor PtoLBD1.2 gene in promoting growth and development of populus tomentosa, a nucleotide sequence of the transcription factor PtoLBD1.2 gene is shown as SEQ ID NO.3. A transgenic plant is overexpressed through transgenic tissue specificity PtoLBD1.2, a plant with high expression quantity is screened out through subsequent positive identification for phenotypic observation, and a result shows that the transcription factor PtoLBD1.2 gene can be used as an overexpression transcription factor PtoLBD1.2 gene for promoting growth and development of populus tomentosa. Compared with the wild type in the same period, the transgenic plant of PtoLBD1.2 has the advantages that the plant height is increased, the stem is thickened, and the aboveground biomass is obviously improved. According to the cultivation method provided by the invention, the high-quality poplar variety with high growth speed and high biomass is cultivated. The method has great significance in molecular breeding and cultivation of excellent poplar in the future.

This invention discloses a method for establishing a high-efficiency leaf regeneration system for aseptic seedlings of Populus tomentosa 'Bofeng No. 3', belonging to the field of agricultural biotechnology. This invention, through 5-AU combined with dark culture treatment, not only induces early leaf differentiation in aseptic seedlings of Populus tomentosa 'Bofeng No. 3', but also significantly increases the number of adventitious buds and the rooting rate. Through chemical treatment and optimized culture conditions, this invention successfully established a high-efficiency plant regeneration system for aseptic leaves of Populus tomentosa 'Bofeng No. 3', laying the foundation for the genetic transformation and gene editing of Populus tomentosa 'Bofeng No. 3', and also providing a reference for the establishment of tissue culture systems for other tree species.

The invention discloses a method for establishing a leaf gene editing system of populus feng No.3 aseptic seedlings, and belongs to the technical field of tissue culture and gene editing. The method comprises the following steps: carrying out 5-AU treatment, establishing and optimizing an efficient genetic transformation system of the populus tomentosa No.3, selecting a specific sgRNAs target, constructing and editing vectors 184kUV-PDS1 and 184kUV-PDS3, and transforming aseptic seedling leaves of the populus tomentosa No.3 with agrobacterium to obtain albino buds. According to the method disclosed by the invention, sgRNA1 is combined with 5-AU for pre-culture for 1d, and 86.11% of genetic transformation efficiency and 53.13% of gene editing efficiency are realized in leaves of aseptic seedlings of the populus feng No.3.

The application belongs to the technical field of genetic engineering, and particularly relates to application of Populus davidiana PtrSCZ1 and PtrSCZ3 genes in regulation of tree vascular cambium development. The problem of tree vascular cambium development is solved. The application includes application of a recombinant vector or an engineering bacterium of the Populus davidiana PtrSCZ1 and PtrSCZ3 genes in regulation of tree vascular cambium development. The application obtains Populus davidiana transgenic plants with overexpression of PtrSCZ1 and PtrSCZ3 through a Populus davidiana genetic transformation system mediated by Agrobacterium. It is found through a series of technical means that overexpression of the PtrSCZ1 and PtrSCZ3 transcription factors makes the Populus davidiana grow slowly, and the number of vascular cambium cell layers is increased. The research content of the application has important theoretical guiding significance for cultivation of high-quality wood tree new varieties.

The application discloses a molecular marker for identifying geographical origin of Populus tomentosa and application thereof, and the molecular marker is located at the 42th base of FPA gene in a poplar genome and has C / A polymorphism, wherein a P. tomentosa individual with a genotype AA of the molecular marker has a shorter annual average sunshine duration, and a P. tomentosa individual with a genotype CC of the molecular marker has a longer annual average sunshine duration. The molecular marker provided by the application has a clear function and a significant effect, and can be directly applied to molecular marker assisted breeding of the poplar; and the molecular marker has environmental adaptability and is suitable for directional introduction of the poplar.

The invention belongs to the technical field of gene engineering, and particularly relates to application of an expression promoter of a PubHLH47 gene to improvement of salt stress resistance of populus japonica, and the nucleotide sequence of the PubHLH47 gene is as shown in SEQ ID NO.1. The PubHLH47 gene provided by the invention is utilized to construct a plant expression vector, so that the PubHLH47 gene is over-expressed in the populus japonica, and the salt stress resistance of the populus japonica is improved. A PubHLH47 gene overexpressed plant has the characteristic of effectively improving the tolerance of the plant under the stress of high salt. The PubHLH47 gene capable of improving the salt stress resistance is found in forest for the first time, an excellent gene resource is provided for promoting forest genetic engineering breeding, and a scientific basis is provided for later improvement of populus nigra.

The present invention discloses the application of overexpressing the auxin transporter PtoPIN1a gene in improving the biomass of Populus tomentosa. The nucleotide sequence of the auxin transporter PtoPIN1a gene is shown as SEQ ID No.1. By using genetic engineering methods, tissue-specific PtoPIN1a overexpressing transgenic plants are obtained after transforming wild-type Populus tomentosa by the Agrobacterium tumefaciens infection method, and plants with higher expression levels are screened out through subsequent positive identification for phenotypic observation. The results show that compared with the wild-type in the same period, the transgenic plants of PtoPIN1a have increased plant height, thicker stems, and significantly improved above-ground biomass. The cultivation technical method provided by the present invention has cultivated high-quality poplar varieties with high biomass. This invention has great significance for future molecular breeding of excellent poplars.

The invention relates to the technical field of forest tree breeding and cultivation, in particular to an integrated method for improved variety breeding, rapid propagation and container seedling cultivation of poplar pie suitable for semi-arid regions. The integration method comprises the following steps: hybridizing populus arabinosa or populus sinkiang as a female parent and populus tomentosa or populus arabinosa as a male parent, screening by using a molecular marker to obtain F1-generation plants with salt-tolerant and drought-resistant characteristics, and sequentially carrying out rapid propagation, container seedling cultivation and afforestation planting on the F1-generation plants. Compared with the prior art, the method can solve the problem of rooting of the poplar pie, the afforestation survival rate is remarkably increased, and the method has the advantages of shortening the breeding period and accurately improving the stress resistance.

The invention relates to the technical field of gene engineering, in particular to application of a populus tomentosa PtTCH4 gene in regulation and control of plant growth and development. The nucleotide sequence of the populus tomentosa PtTCH4 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the protein coded by the populus tomentosa PtTCH4 gene is as shown in SEQ ID NO.2. Through overexpression of the PtTCH4 gene, the inflorescence color of a transgenic plant is lighter than that of a wild plant, and meanwhile, the inflorescence is increased; the regulation of plant inflorescence development is of great significance to promotion of plant genetic improvement and breeding.

The invention relates to a group of SNP (Single Nucleotide Polymorphism) molecular markers associated with surface area characters of poplar roots and application, and belongs to the technical field of genetic breeding of poplar. The method comprises the following steps: identifying key sites influencing the root surface area through whole genome association analysis, and respectively positioning the key sites at the 10391007bp (SAsite1) and the 10391759bp (SAsite2) on the No.6 chromosome of a populus sibirica genome; the two sites are completely linked, and the root surface area of the heterozygous haplotype (GA / CG) is obviously larger than that of the homozygous haplotype (GG / CC). The molecular marker provided by the invention can be used for early molecular auxiliary selection of the surface area of the root of the poplar, is beneficial to accurately and efficiently screening a poplar clone with a relatively large root surface area, and provides reliable information for improving the survival rate of vegetative propagation and breeding an excellent strain.

The present invention relates to the use of the overexpression of a Populus tomentosa strigolactone receptor PtoD14 gene in increasing a wood yield. By means of cloning a Populus tomentosa strigolactone receptor PtoD14 gene from wild-type Populus tomentosa and introducing the PtoD14 gene into Populus tomentosa plants by means of a genetic engineering method, a Populus tomentosa line with enhanced secondary development is obtained. The number of secondary xylem cell layers in 3-month-old PtoD14 transgenic Populus tomentosa is 8 to 21 layers more than that in 3-month-old non-transgenic common Populus tomentosa, which is of important significance for providing a high wood yield for the large-scale production of Populus tomentosa.

The invention discloses a method, a system, equipment, a medium and a product for predicting the wadding floating period of willow, and relates to the field of environmental pollution, and the method comprises the steps: constructing wadding floating period prediction models corresponding to different types of willow based on a unary linear regression modeling method; the different willow types comprise salix matsudana, populus tomentosa and black populus tomentosa; obtaining the activity accumulated temperature of different willow types in the current year; and inputting the current-year activity accumulated temperatures of different willow types into the corresponding willow wadding floating period prediction models to obtain predicted willow wadding floating period daily ordinal numbers corresponding to the different willow types, so that the accuracy of predicting the willow wadding floating period can be improved, accurate willow wadding floating period time is provided for municipal and garden departments, and the method is suitable for popularization and application. Therefore, pollution caused by poplar catkins is prevented in advance, and poplar catkins harm is reduced.

The present invention discloses the application and method of the PtobZIP29 gene in regulating the wood development of white poplar. By overexpressing the PtobZIP29 gene in white poplar, the division activity of the vascular cambium of the poplar is promoted, and the formation of secondary xylem is accelerated, thereby obtaining transgenic white poplar germplasm with increased wood biomass and improved wood fast-growing ability. This provides a method and germplasm basis for the breeding of fast-growing and superior forest varieties, is conducive to improving the wood yield and economic benefits of economic forests, and also provides materials for the construction of shelter forests and urban and rural greening.

The invention belongs to the technical field of biology, and particularly relates to application of a PtoHSFC1 gene in regulating and controlling cold resistance and heat resistance of a poplar, and the nucleotide sequence of the PtoHSFC1 gene is shown as SEQ ID NO.1. Through bioinformatics analysis, inducible expression analysis, target gene overexpression and temperature treatment of a knockout plant, the result shows that the heat resistance and cold resistance of the populus tomentosa can be improved by improving the expression level of the PtoHSFC1 gene.

The invention provides a PtoPHA14D gene for improving drought resistance of plants and application of the PtoPHA14D gene, and belongs to the technical field of gene engineering. The overexpression of the PtoPHA14D gene promotes the generation of adventitious roots of the populus tomentosa, enriches the root structure of the populus tomentosa, and further enhances the tolerance of the populus tomentosa to drought stress. The influence of overexpression PtoPHA14D on the external growth phenotype of the plant is mainly reflected, and the PtoPHA14D has a regulation effect on the adventitious roots of the populus tomentosa: the number and length of the adventitious roots of the transgenic plant are increased, the drought resistance is enhanced, and malondialdehyde and the relative conductivity value are remarkably reduced. The PtoPHA14D gene disclosed by the invention has important theoretical guiding significance for cultivating new varieties of high-quality forest trees.

This invention relates to the field of genetic breeding technology, and specifically discloses a type of Populus tomentosa. PdbRAP2 Genes and their applications, the aforementioned PdbRAP2 The CDS sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence it encodes is shown in SEQ ID NO.2. This invention utilizes genetic engineering to overexpress *Populus sanguisorbus*. PdbRAP2 Genes have improved the drought resistance of Populus tomentosa.

The present invention discloses PCR primers, a detection method, and a PCR detection kit for detecting the genome of Xinjiang poplar. Based on the genomes of Populus alba, Xinjiang poplar, Populus rapa, and Populus tremula, the present invention finds fragments with large differences in the genome through whole genome sequence comparison analysis, and designs specific primers for each poplar accordingly, from which specific primers that accurately distinguish the Xinjiang poplar genome from the genomes of other poplars are screened. The present invention further provides a PCR detection method and a detection kit for quickly distinguishing Xinjiang poplar from other poplar species using the specific primers. The present invention can quickly distinguish Xinjiang poplar from other poplar species. The PCR detection method is easy to operate and has a simple banding pattern. It does not require sequencing to directly distinguish Xinjiang poplar-specific genomic fragments by amplifying fragments. It has application prospects in the classification and utilization of poplar planting resources and the identification of hybrids.

The present invention discloses the application of the PtoELF3.1 gene in creating non-dormant evergreen poplars, belonging to the fields of biotechnology and molecular biology. The poplar ELF3.1 gene is a protein encoding the amino acid sequence shown in SEQ ID No. 1. By targeting the PtoELF3.1 gene with the CRISPR / Cas9 gene editing system, a Ptoelf3.1 mutant strain of Populus tomentosa was obtained. Compared with wild-type (WT) Populus tomentosa plants, the Ptoelf3.1 mutant plants still maintained an evergreen phenotype with non-dormant terminal and lateral buds under short-day conditions in autumn and winter. The present invention shows that knocking out the PtoELF3.1 gene causes the poplar to be insensitive to short-day conditions and lacks a response to seasonal changes, thereby maintaining an evergreen, non-dormant growth state. The present invention has great application prospects in improving photosynthetic carbon fixation in forests, increasing timber yield, and creating ornamental tree germplasm.

The invention provides a molecular marker closely linked with a populus tomentosa seedling stage growth vigor character. The method can accurately evaluate the growth vigor of the populus tomentosa at the molecular level in the seedling stage, high-yield new populus germplasm can be bred through an efficient molecular marker-assisted breeding means in the seedling stage, and compared with mature-stage adult breeding in traditional industrial breeding, the method greatly shortens the populus breeding period and improves the breeding efficiency of populus tomentosa. And an efficient means is provided for improved variety breeding of industrial materials of populus tomentosa.

The application discloses application and a method of knocking out a SHOU4 gene of Populus tomentosa in improving wood biomass, and a new poplar variety with high cellulose, high biomass, significantly increased xylem cell layers and fiber cell wall thickness is successfully created by knocking out the SHOU4 gene. The application lays a foundation for genetic improvement and directional molecular breeding of forest biomass, provides a new gene resource for forest gene engineering, and has a wide application prospect.

The invention belongs to the technical field of machine learning, and provides a poplar callus ultralow-temperature preservation method based on machine learning and molecular dynamics simulation, and the main scheme is as follows: constructing a poplar stress-resistant phenotype prediction model based on machine learning, screening out poplar with stress-resistant phenotype based on the poplar stress-resistant phenotype prediction model, and storing the poplar callus ultralow-temperature preservation method based on machine learning and molecular dynamics simulation. The tissue is used as a populus albus callus tissue material; the method comprises the following steps: determining pre-culture medium components of a populus albus callus material based on molecular dynamics simulation, and performing pre-culture treatment on the populus albus callus material by using a determined pre-culture medium; a cryoprotectant is screened based on molecular dynamics simulation, and the screened cryoprotectant is used for conducting freezing treatment on the populus albus callus material subjected to pre-culture treatment; and determining a thawing mode based on molecular dynamics simulation, and unfreezing and re-culturing the frozen populus albus callus material by using the determined thawing mode.

The invention relates to a populus tomentosa PtoYABBY2 gene and application thereof, and belongs to the field of plant genetic engineering. The nucleotide sequence of the gene is as shown in Seq ID NO.1, and the gene encodes a protein of Seq ID NO.2. The gene can obviously advance the flowering time of plants by regulating and controlling key factors (such as up-regulation of AtFT and the like and inhibition of AtFLC) of a flowering pathway. Through overexpression of the PtoYABBY2 in arabidopsis thaliana, the flowering time can be advanced by about 4 days on average, and floral organs grow normally. The gene shows tissue specific expression (mature leaves > young leaves > stems / roots) and dynamic expression in a flower bud development stage in populus tomentosa. The invention provides a construction method of a cloning primer, a fluorescent quantitative primer and an overexpression vector. The invention also discloses a method for cultivating populus tomentosa varieties with different flowering time by using the gene. By applying the gene, the breeding cycle of populus tomentosa can be shortened, the problems of long juvenile phase and flying catkins pollution are solved, and a new tool is provided for forest molecular design breeding.

The invention discloses a target gene, a PCR (Polymerase Chain Reaction) detection primer, a kit and a PCR identification method for identifying the gender of populus tomentosa. Aiming at the technical bottleneck that early phenotypes of male and female plants of populus tomentosa are difficult to distinguish, a gender-specific nucleotide sequence linked with characters of populus tomentosa is obtained by screening through a differential phenotype grouping strategy by adopting a cluster separation analysis method, and a specific PCR primer pair is obtained by screening based on the gender-specific sequence; on the basis, a PCR detection method or a detection kit for accurately distinguishing male and female plants is established, large-scale sample verification shows that the accuracy rate of sex identification of the populus tomentosa reaches 100%, and efficient and accurate sex identification of the populus tomentosa seedling-stage plants is realized. The detection method disclosed by the invention has the characteristics of high accuracy, simplicity and convenience in operation and high repeatability, and has application prospects in the aspects of improved variety breeding of populus tomentosa, directional cultivation management, germplasm resource library construction, sex-related gene function research and the like.

The invention further discloses application of the overexpression auxin transporter PtoPIN1d gene in improving the growth index of the populus tomentosa. The invention discloses application of an overexpressed auxin transporter PtoPIN1d gene in increasing the length of wood fibers of populus tomentosa. The nucleotide sequence of the auxin transporter PtoPIN1d gene is shown as SEQ ID No.2. A genetic engineering method is adopted, wild populus tomentosa is transformed through an agrobacterium tumefaciens infection method, then a tissue-specific PtoPIN1d overexpressed transgenic plant is obtained, and the tissue-specific PtoPIN1d overexpressed transgenic plant is obtained. And screening out plants with relatively high expression quantity for phenotypic observation through subsequent positive identification. Results show that compared with a wild type in the same period, the transgenic plant of PtoPIN1d has the advantages that the plant height is increased, xylem development is accelerated, xylem cells are enlarged, and the fiber length of mature wood is remarkably increased. According to the cultivation technology method provided by the invention, the high-quality poplar variety with long wood fibers is cultivated. The method has great significance in molecular breeding and cultivation of excellent poplar in the future.

The invention discloses application of a populus tomentosa PtomABI1a gene in maintaining cambium activity and promoting xylem development, and belongs to the field of genetic engineering.An LMX5pro: PtomABI1a expression vector is transferred into populus tomentosa, compared with a wild type, transgenic populus tomentosa over-expressing PtomABI1a in cambium and xylem areas has the advantages that growth stop of terminal buds is slightly delayed after short-day treatment, and growth of the transgenic populus tomentosa over-expressing PtomABI1a in the cambium and xylem areas is delayed; after short-day and low-temperature treatment, the internode number is remarkably increased, the xylem is remarkably increased, and the sensitivity to short-day and low-temperature treatment is reduced. The results show that the use of the LMX5pro promoter for specifically weakening ABA signals in cambium and xylem is crucial to regulation of cambium activity and wood development of populus tomentosa under environmental stress. The invention provides a new gene source for regulating and controlling the activity of a tree cambium and wood development, and has an important application value in the field of forest tree gene engineering.

This invention provides a salt-tolerant Populus tomentosa RHB1A This invention relates to genes and their applications, belonging to the field of genetic engineering technology. The salt-tolerant gene from Populus tomentosa provided by this invention... RHB1A The nucleotide sequence is shown in SEQ ID NO.1, and the encoded amino acid sequence is shown in SEQ ID NO.2. This invention screened an E3 ubiquitin ligase RHB1A and cloned its full-length CDS sequence. This was then demonstrated by overexpression in Populus tomentosa plants. RHB1A The gene significantly improved the plant's salt tolerance, providing theoretical support for the breeding and widespread promotion of salt-tolerant tree varieties.