25 results about "Populus tomentosa" patented technology

The invention relates to the technical field of plant genetic engineering, in particular to an application of a poplar PtbHLH47 gene and an encoding protein thereof in improving the salt tolerance of plants. The invention discloses application of a PtbHLH47 gene to improvement of plant salt tolerance and improvement of chlorophyll content of plant leaves. The nucleotide sequence of the PtbHLH47 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the expression protein of the PtbHLH47 gene is as shown in SEQ ID NO. 2. PtbHLH47 genes are cloned from populus tomentosa leaves, overexpressed PtbHLH47 transgenic populus tomentosa is obtained through an agrobacterium tumefaciens-mediated populus genetic transformation system, salt tolerance evaluation shows that the salt tolerance of the overexpressed PtbHLH47 transgenic populus tomentosa is remarkably improved, particularly, the chlorophyll content in the populus tomentosa leaves under salt stress can be remarkably improved, and the chlorophyll content in the populus tomentosa leaves under salt stress can be remarkably reduced. And gene resources are provided for cultivating salt-tolerant plants.

This invention discloses a method for establishing a high-efficiency leaf regeneration system for aseptic seedlings of Populus tomentosa 'Bofeng No. 3', belonging to the field of agricultural biotechnology. This invention, through 5-AU combined with dark culture treatment, not only induces early leaf differentiation in aseptic seedlings of Populus tomentosa 'Bofeng No. 3', but also significantly increases the number of adventitious buds and the rooting rate. Through chemical treatment and optimized culture conditions, this invention successfully established a high-efficiency plant regeneration system for aseptic leaves of Populus tomentosa 'Bofeng No. 3', laying the foundation for the genetic transformation and gene editing of Populus tomentosa 'Bofeng No. 3', and also providing a reference for the establishment of tissue culture systems for other tree species.

The invention discloses a method for establishing a leaf gene editing system of populus feng No.3 aseptic seedlings, and belongs to the technical field of tissue culture and gene editing. The method comprises the following steps: carrying out 5-AU treatment, establishing and optimizing an efficient genetic transformation system of the populus tomentosa No.3, selecting a specific sgRNAs target, constructing and editing vectors 184kUV-PDS1 and 184kUV-PDS3, and transforming aseptic seedling leaves of the populus tomentosa No.3 with agrobacterium to obtain albino buds. According to the method disclosed by the invention, sgRNA1 is combined with 5-AU for pre-culture for 1d, and 86.11% of genetic transformation efficiency and 53.13% of gene editing efficiency are realized in leaves of aseptic seedlings of the populus feng No.3.

The application belongs to the technical field of genetic engineering, and particularly relates to application of Populus davidiana PtrSCZ1 and PtrSCZ3 genes in regulation of tree vascular cambium development. The problem of tree vascular cambium development is solved. The application includes application of a recombinant vector or an engineering bacterium of the Populus davidiana PtrSCZ1 and PtrSCZ3 genes in regulation of tree vascular cambium development. The application obtains Populus davidiana transgenic plants with overexpression of PtrSCZ1 and PtrSCZ3 through a Populus davidiana genetic transformation system mediated by Agrobacterium. It is found through a series of technical means that overexpression of the PtrSCZ1 and PtrSCZ3 transcription factors makes the Populus davidiana grow slowly, and the number of vascular cambium cell layers is increased. The research content of the application has important theoretical guiding significance for cultivation of high-quality wood tree new varieties.

The application discloses a molecular marker for identifying geographical origin of Populus tomentosa and application thereof, and the molecular marker is located at the 42th base of FPA gene in a poplar genome and has C / A polymorphism, wherein a P. tomentosa individual with a genotype AA of the molecular marker has a shorter annual average sunshine duration, and a P. tomentosa individual with a genotype CC of the molecular marker has a longer annual average sunshine duration. The molecular marker provided by the application has a clear function and a significant effect, and can be directly applied to molecular marker assisted breeding of the poplar; and the molecular marker has environmental adaptability and is suitable for directional introduction of the poplar.

The invention belongs to the technical field of gene engineering, and particularly relates to application of an expression promoter of a PubHLH47 gene to improvement of salt stress resistance of populus japonica, and the nucleotide sequence of the PubHLH47 gene is as shown in SEQ ID NO.1. The PubHLH47 gene provided by the invention is utilized to construct a plant expression vector, so that the PubHLH47 gene is over-expressed in the populus japonica, and the salt stress resistance of the populus japonica is improved. A PubHLH47 gene overexpressed plant has the characteristic of effectively improving the tolerance of the plant under the stress of high salt. The PubHLH47 gene capable of improving the salt stress resistance is found in forest for the first time, an excellent gene resource is provided for promoting forest genetic engineering breeding, and a scientific basis is provided for later improvement of populus nigra.

The invention relates to the technical field of forest tree breeding and cultivation, in particular to an integrated method for improved variety breeding, rapid propagation and container seedling cultivation of poplar pie suitable for semi-arid regions. The integration method comprises the following steps: hybridizing populus arabinosa or populus sinkiang as a female parent and populus tomentosa or populus arabinosa as a male parent, screening by using a molecular marker to obtain F1-generation plants with salt-tolerant and drought-resistant characteristics, and sequentially carrying out rapid propagation, container seedling cultivation and afforestation planting on the F1-generation plants. Compared with the prior art, the method can solve the problem of rooting of the poplar pie, the afforestation survival rate is remarkably increased, and the method has the advantages of shortening the breeding period and accurately improving the stress resistance.

The invention relates to the technical field of gene engineering, in particular to application of a populus tomentosa PtTCH4 gene in regulation and control of plant growth and development. The nucleotide sequence of the populus tomentosa PtTCH4 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the protein coded by the populus tomentosa PtTCH4 gene is as shown in SEQ ID NO.2. Through overexpression of the PtTCH4 gene, the inflorescence color of a transgenic plant is lighter than that of a wild plant, and meanwhile, the inflorescence is increased; the regulation of plant inflorescence development is of great significance to promotion of plant genetic improvement and breeding.

The invention relates to a group of SNP (Single Nucleotide Polymorphism) molecular markers associated with surface area characters of poplar roots and application, and belongs to the technical field of genetic breeding of poplar. The method comprises the following steps: identifying key sites influencing the root surface area through whole genome association analysis, and respectively positioning the key sites at the 10391007bp (SAsite1) and the 10391759bp (SAsite2) on the No.6 chromosome of a populus sibirica genome; the two sites are completely linked, and the root surface area of the heterozygous haplotype (GA / CG) is obviously larger than that of the homozygous haplotype (GG / CC). The molecular marker provided by the invention can be used for early molecular auxiliary selection of the surface area of the root of the poplar, is beneficial to accurately and efficiently screening a poplar clone with a relatively large root surface area, and provides reliable information for improving the survival rate of vegetative propagation and breeding an excellent strain.

The present invention discloses the application and method of the PtobZIP29 gene in regulating the wood development of white poplar. By overexpressing the PtobZIP29 gene in white poplar, the division activity of the vascular cambium of the poplar is promoted, and the formation of secondary xylem is accelerated, thereby obtaining transgenic white poplar germplasm with increased wood biomass and improved wood fast-growing ability. This provides a method and germplasm basis for the breeding of fast-growing and superior forest varieties, is conducive to improving the wood yield and economic benefits of economic forests, and also provides materials for the construction of shelter forests and urban and rural greening.

The invention belongs to the technical field of biology, and particularly relates to application of a PtoHSFC1 gene in regulating and controlling cold resistance and heat resistance of a poplar, and the nucleotide sequence of the PtoHSFC1 gene is shown as SEQ ID NO.1. Through bioinformatics analysis, inducible expression analysis, target gene overexpression and temperature treatment of a knockout plant, the result shows that the heat resistance and cold resistance of the populus tomentosa can be improved by improving the expression level of the PtoHSFC1 gene.

The invention provides a PtoPHA14D gene for improving drought resistance of plants and application of the PtoPHA14D gene, and belongs to the technical field of gene engineering. The overexpression of the PtoPHA14D gene promotes the generation of adventitious roots of the populus tomentosa, enriches the root structure of the populus tomentosa, and further enhances the tolerance of the populus tomentosa to drought stress. The influence of overexpression PtoPHA14D on the external growth phenotype of the plant is mainly reflected, and the PtoPHA14D has a regulation effect on the adventitious roots of the populus tomentosa: the number and length of the adventitious roots of the transgenic plant are increased, the drought resistance is enhanced, and malondialdehyde and the relative conductivity value are remarkably reduced. The PtoPHA14D gene disclosed by the invention has important theoretical guiding significance for cultivating new varieties of high-quality forest trees.

This invention relates to the field of genetic breeding technology, and specifically discloses a type of Populus tomentosa. PdbRAP2 Genes and their applications, the aforementioned PdbRAP2 The CDS sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence it encodes is shown in SEQ ID NO.2. This invention utilizes genetic engineering to overexpress *Populus sanguisorbus*. PdbRAP2 Genes have improved the drought resistance of Populus tomentosa.

The present invention discloses PCR primers, a detection method, and a PCR detection kit for detecting the genome of Xinjiang poplar. Based on the genomes of Populus alba, Xinjiang poplar, Populus rapa, and Populus tremula, the present invention finds fragments with large differences in the genome through whole genome sequence comparison analysis, and designs specific primers for each poplar accordingly, from which specific primers that accurately distinguish the Xinjiang poplar genome from the genomes of other poplars are screened. The present invention further provides a PCR detection method and a detection kit for quickly distinguishing Xinjiang poplar from other poplar species using the specific primers. The present invention can quickly distinguish Xinjiang poplar from other poplar species. The PCR detection method is easy to operate and has a simple banding pattern. It does not require sequencing to directly distinguish Xinjiang poplar-specific genomic fragments by amplifying fragments. It has application prospects in the classification and utilization of poplar planting resources and the identification of hybrids.

The present invention discloses the application of the PtoELF3.1 gene in creating non-dormant evergreen poplars, belonging to the fields of biotechnology and molecular biology. The poplar ELF3.1 gene is a protein encoding the amino acid sequence shown in SEQ ID No. 1. By targeting the PtoELF3.1 gene with the CRISPR / Cas9 gene editing system, a Ptoelf3.1 mutant strain of Populus tomentosa was obtained. Compared with wild-type (WT) Populus tomentosa plants, the Ptoelf3.1 mutant plants still maintained an evergreen phenotype with non-dormant terminal and lateral buds under short-day conditions in autumn and winter. The present invention shows that knocking out the PtoELF3.1 gene causes the poplar to be insensitive to short-day conditions and lacks a response to seasonal changes, thereby maintaining an evergreen, non-dormant growth state. The present invention has great application prospects in improving photosynthetic carbon fixation in forests, increasing timber yield, and creating ornamental tree germplasm.

The application discloses application and a method of knocking out a SHOU4 gene of Populus tomentosa in improving wood biomass, and a new poplar variety with high cellulose, high biomass, significantly increased xylem cell layers and fiber cell wall thickness is successfully created by knocking out the SHOU4 gene. The application lays a foundation for genetic improvement and directional molecular breeding of forest biomass, provides a new gene resource for forest gene engineering, and has a wide application prospect.

The invention belongs to the technical field of machine learning, and provides a poplar callus ultralow-temperature preservation method based on machine learning and molecular dynamics simulation, and the main scheme is as follows: constructing a poplar stress-resistant phenotype prediction model based on machine learning, screening out poplar with stress-resistant phenotype based on the poplar stress-resistant phenotype prediction model, and storing the poplar callus ultralow-temperature preservation method based on machine learning and molecular dynamics simulation. The tissue is used as a populus albus callus tissue material; the method comprises the following steps: determining pre-culture medium components of a populus albus callus material based on molecular dynamics simulation, and performing pre-culture treatment on the populus albus callus material by using a determined pre-culture medium; a cryoprotectant is screened based on molecular dynamics simulation, and the screened cryoprotectant is used for conducting freezing treatment on the populus albus callus material subjected to pre-culture treatment; and determining a thawing mode based on molecular dynamics simulation, and unfreezing and re-culturing the frozen populus albus callus material by using the determined thawing mode.

The invention relates to a populus tomentosa PtoYABBY2 gene and application thereof, and belongs to the field of plant genetic engineering. The nucleotide sequence of the gene is as shown in Seq ID NO.1, and the gene encodes a protein of Seq ID NO.2. The gene can obviously advance the flowering time of plants by regulating and controlling key factors (such as up-regulation of AtFT and the like and inhibition of AtFLC) of a flowering pathway. Through overexpression of the PtoYABBY2 in arabidopsis thaliana, the flowering time can be advanced by about 4 days on average, and floral organs grow normally. The gene shows tissue specific expression (mature leaves > young leaves > stems / roots) and dynamic expression in a flower bud development stage in populus tomentosa. The invention provides a construction method of a cloning primer, a fluorescent quantitative primer and an overexpression vector. The invention also discloses a method for cultivating populus tomentosa varieties with different flowering time by using the gene. By applying the gene, the breeding cycle of populus tomentosa can be shortened, the problems of long juvenile phase and flying catkins pollution are solved, and a new tool is provided for forest molecular design breeding.

This invention provides a salt-tolerant Populus tomentosa RHB1A This invention relates to genes and their applications, belonging to the field of genetic engineering technology. The salt-tolerant gene from Populus tomentosa provided by this invention... RHB1A The nucleotide sequence is shown in SEQ ID NO.1, and the encoded amino acid sequence is shown in SEQ ID NO.2. This invention screened an E3 ubiquitin ligase RHB1A and cloned its full-length CDS sequence. This was then demonstrated by overexpression in Populus tomentosa plants. RHB1A The gene significantly improved the plant's salt tolerance, providing theoretical support for the breeding and widespread promotion of salt-tolerant tree varieties.

The invention belongs to the technical field of insect-resistant research, and particularly relates to application of a small-molecule compound SPD to resistance of hyphantria cunea pests of poplars. Comprising the following steps: (1) taking a plurality of populus tomentosa plant seedlings growing for 3 months in a greenhouse; (2) adding water into the spermidine solution in proportion, and respectively diluting the spermidine solution into working concentrations of 0.5 mM, 1.0 mM, 2.0 mM and 4.0 mM; (3) taking the diluted spermidine solution as a treatment group and an aqueous solution group or a control group, respectively spraying the leaves of the poplar twice a day, continuously spraying for 3 days at the interval of 6 hours, and then culturing in a greenhouse; and (4) 36 hours after the last time of spraying, taking the seventh leaf and the contrast, and carrying out a hyphantria cunea feeding experiment. According to the method, a new green protection strategy is provided for the artificial forest to cope with the fall webworm pests, pesticide dependence is reduced, and agriculture and forestry and sustainable development are promoted.

The present invention discloses the application and method of overexpressing the PtoCRY2 gene in delaying the dormancy of the terminal bud of Populus tomentosa and promoting wood development. By transferring the PtoCRY2 gene into wild-type (WT) plants of Populus tomentosa, the plant height and biomass of the PtoCRY2-OE overexpressing plants of Populus tomentosa are significantly increased under short photoperiod conditions compared with the WT plants. Compared with WT, the PtoCRY2-OE overexpressing plants of Populus tomentosa have a longer growth period, which is manifested by the delay in the time of cessation of terminal bud growth and entry into dormancy. This shows that the blue light receptor PtoCRY2 is an important regulatory factor that promotes poplar growth and wood development. The present invention provides ideas and references for promoting poplar wood yield and ecological adaptability using the PtoCRY2 gene, and has important application value in the field of forest genetic engineering.

The invention relates to a populus tomentosa PtoYABBY12 gene and application thereof, and belongs to the field of plant genetic engineering. The nucleotide sequence of the gene is as shown in Seq ID NO.1, and an amino acid sequence as shown in Seq ID NO.2 is encoded. The gene plays a key role in regulating and controlling the flowering time of plants, and the flowering time can be obviously advanced through overexpression. The invention provides a specific primer pair for cloning the gene, a fluorescent quantitative primer pair and a constructed overexpression vector. Through arabidopsis thaliana transgenosis verification, the flowering time of a plant over-expressing PtoYABBY12 is advanced by about 4 days compared with that of a wild type, and regulation and control are realized by up-regulating a flowering promoting gene and inhibiting AtFLC expression. The gene is highly expressed in a specific development period of female / male flower buds of populus tomentosa and in leaves, and is positioned in a cell nucleus. By applying the gene, the breeding cycle of populus tomentosa can be shortened, a new variety with improved flowering time can be cultivated, and technical support is provided for molecular design breeding.

The invention discloses a poplar cold-resistant whole genome SNP site combination, a probe, a chip and application thereof, and relates to the technical field of molecular breeding. The invention provides a cold-resistant whole genome SNP (Single Nucleotide Polymorphism) site combination of a poplar. The cold-resistant whole genome SNP site combination comprises 40141 SNP sites. According to the method, 639 poplar re-sequencing materials are utilized, a plurality of algorithms are selected to correlate with the cold resistance related SNP markers, whole genome selection models of different data sets are constructed, and the prediction effect is good. The marker combination is distributed on a whole genome, can provide theoretical and data support for the cold-resistant breeding work of the poplar, has relatively important theoretical value and application significance, and effectively solves the problems that the traditional cold-resistant breeding of the poplar depends on manual selection, the workload is large and the efficiency is low.

The invention discloses an efficient genetic transformation method suitable for Sibin 1, Nanlin 895 poplar and populus tomentosa, and relates to the technical field of plant genetic engineering. An efficient genetic transformation system is established by taking a stem segment or a root segment of a poplar as an explant through the steps of preparation, agrobacterium infection and co-culture, recovery culture, resistance screening and bud induction, rooting culture and positive plant identification. Wherein the stem explant preparation comprises the following steps: taking 1-2cm of sterile tissue culture seedling stem, and removing axillary buds and epidermis on one side; the step of preparing the explant of the root section comprises the steps of taking the root section of 1-2cm and cutting off thin lateral roots. By optimizing the infection liquid and the culture medium formula in each stage, the browning rate is remarkably reduced, the transformation period is shortened, the positive rate of different varieties is improved, the operation is simple and convenient, the cost is low, and efficient and universal technical support is provided for poplar gene function research and molecular breeding.

The present invention discloses a molecular marker for accurately predicting the spring bud break time of Populus tomentosa. The molecular marker is located at position 395 of the nucleotide sequence shown in SEQ ID NO: 1 and has a G / T polymorphism. Populus tomentosa individuals with a genotype of GG have a late spring bud break time, Populus tomentosa individuals with a genotype of TT have an early spring bud break time, and Populus tomentosa individuals with a genotype of GT have a moderate spring bud break time. The molecular marker for accurately predicting the spring bud break time provided by the present invention has a clear function and significant effect, and can be directly applied to molecular marker-assisted breeding of poplars, significantly improving breeding efficiency and shortening the breeding cycle of poplars.