87 results about "Phloem" patented technology

The invention discloses an application of a populus tomentosa IPT5b gene in creating a high-yield shade-tolerant populus germplasm, belongs to forest breeding, and is characterized in that a phloem specific promoter CLE41b is utilized to express a cytokinin synthetic gene IPT5b in populus tomentosa, and an overexpression material of which cambium activity and xylem development are remarkably promoted is created. After shading treatment, the number of cambium cell layers and the number of xylem cell layers of CLE41bpro: IPT5b are remarkably higher than those of wild type CLE41bpro: IPT5b, and the phenotype increases the content of cytokinin (CK) in stems, so that the wood yield of trees under the shading condition can be increased. According to the method, a high-yield and shade-tolerant new poplar variety is created in a manner of finely regulating and controlling the content of CK in the phloem of the poplar. The gene source and scientific reference are provided for improving and improving the yield and shade tolerance of other trees, and the gene has important application value in the field of forest tree gene engineering.

The present invention provides methods and compositions for targeted delivery of chemical or biomolecular cargo and / or nanoparticles to plant phloem or fungi.

The invention belongs to the technical field of agriculture, and particularly relates to a method for promoting growth and development of rice based on illumination and nitrogen. One of the purposes of the invention is to provide a method for promoting growth and development of rice based on illumination and nitrogen. The method comprises the following steps: providing cultivation conditions of illumination intensity of 600-800 [mu] mol.m <-2 >. S <-1 > and nitrogen concentration of 5.51-8.11 mM for rice in a tillering stage of the rice. By accurately applying a specific combination of illumination intensity and nitrogen concentration in the tillering stage of rice, efficient loading and long-distance transportation of photosynthetic products (especially cane sugar and the like) in phloem are promoted, and the photosynthetic products are guided to be more effectively and directionally distributed to developed library organs (such as fruits, grains or root systems); therefore, the transfer and circulation efficiency of carbon-nitrogen substances in plants is improved synergistically on the whole, the waste phenomenon of nitrogen redundancy or local lack and ineffective accumulation of photosynthetic products is reduced, and finally, an important physiological regulation basis is provided for remarkably improving the yield and quality of crops.

The invention discloses application of a PdeTLP protein and a related biological material thereof in regulation and control of plant root system development. Experiments prove that compared with wild poplar, the number of adventitious roots, the number of adventitious root lateral roots, the root length and the root included angle of PdeTLP knockout poplar are obviously increased, and the plant height, the number of stem nodes and the stem diameter are also obviously increased. In addition, the xylem width, the phloem width and the xylem cell area of the poplar with the PdeTLP knocked out are obviously higher than those of the wild poplar. The PdeTLP gene participates in regulation and control of the root system number, the root system length, the root system included angle, the plant height, the stem node number, the stem diameter and the secondary microtubule tissue development of the poplar. The method not only has important significance on molecular mechanism and theoretical research of regulating and controlling the number and length of poplar root systems, plant height, number of stem nodes and stem diameter, but also has important guiding significance on directionally cultivating trees meeting actual production requirements.

The invention relates to a phenyl thiazole formic acid compound containing an aromatic amide structure and a preparation method and application thereof.In the phenyl thiazole formic acid compound containing the aromatic amide structure, a carboxylic acid group is beneficial to achieving phloem conductivity, and an amide active substructure introduced into an amino site effectively improves the bactericidal activity of the compound; therefore, the compound disclosed by the invention not only shows excellent agricultural fungicidal activity, but also has the capability of conducting in the phloem of the plant. The raw materials are easy to obtain and process, various pesticide formulations can be prepared, and the requirements of different conditions and different use scenes can be met. The specific structure of the phenyl thiazole formic acid compound is shown as a formula I in the specification.

The invention discloses a healing promoting agent for melon vegetable grafted seedlings. The healing promoting agent contains abscisic acid and ribulose phosphate. The effect of promoting communication of phloem and xylem of grafted seedlings can be achieved by spraying the healing promoting agent before grafting, the healing time of the melon vegetable grafted seedlings is shortened, the operation of film uncovering and film covering in the healing period is reduced, the healing period management is simplified, and the healing cost is reduced.

The application discloses a solid-phase extraction agent and a preparation method and application thereof. Raw materials of the solid-phase extraction agent include cigar stems and shavings, and the mass ratio of the cigar stems to the shavings is 1:1-2. The above raw materials are all natural plants, and in addition to being rich in vascular systems and cellulose, the structure formed by xylem and phloem arranged in bundles and a variety of capillary pore structures make the pores rich, and the structure is provided with rich polar hydroxyl and hydrophobic aromatic functional groups. After being prepared as the solid-phase extraction agent, the structure can form pi-pi bonds and hydrogen bonds with benzo[a]pyrene, has strong adsorption capacity for the benzo[a]pyrene and high adsorption stability, and due to the rich pores, has good permeability. The speed of the benzo[a]pyrene in the solution after adsorption through the extraction column is faster, the adsorption enrichment and purification efficiency of the benzo[a]pyrene in the solution after adsorption is high, and the cost is low and the structure is green and environment-friendly.

The invention discloses a microbial fertilizer for treating citrus huanglongbing and a preparation method of the microbial fertilizer, and belongs to the field of agricultural biological control. 33 to 50 percent of sodium alginate-chitosan microspheres; 5 to 8% of hesperidin; 3-5% of nano zinc oxide; 2-4% of humic acid chelated boron; 2-4% of sucrose fatty acid ester; 2-4% of diatomite; wherein the preparation method of the sodium alginate-chitosan microspheres comprises the following steps: mixing sodium alginate with a microbial suspension, dropwise adding the mixture into a calcium chloride solution by using an injector, and standing and curing to form a microsphere core; and immersing the cured microspheres into a chitosan solution with the pH value of 6.0, taking out, and freeze-drying to obtain finished microspheres. Wherein the microbial suspension comprises trichoderma, bacillus and actinomycetes. The invention solves the problems of poor prevention and treatment effect of the existing Candidatus Liberobacter asiaticum, difficulty in colonization of the strain at phloem, short survival time and lack of targeted slow release function.

The application discloses a propiconazole-azoxystrobin- clothianidin seed treatment suspending agent and a preparation method and application thereof, and belongs to the technical field of pesticide preparations; the agent takes propiconazole, azoxystrobin and clothianidin as active ingredients, takes beta-nicotinamide mononucleotide (NMN) as a functional antimicrobial component, and is supplemented with styrene-maleic anhydride copolymer sodium salt, fatty alcohol polyoxyethylene ether, xanthan gum, magnesium aluminum silicate, glycerol, polydimethylsiloxane defoaming agent, sodium benzoate, polyvinyl alcohol and deionized water to prepare the agent. The application is suitable for seed coating treatment for preventing and treating rice false smut, thrips and wheat sharp eyespot and aphids; NMN promotes the accumulation of phenolic compounds in the phloem of plants, and cooperates with clothianidin on the same insecticidal target, thereby significantly improving the prevention and treatment effect on aphids and thrips; the degradation product of NMN promotes the proliferation of beneficial microorganisms in the rhizosphere, improves soil enzyme activity, and realizes soil micro-ecological restoration.

The invention provides a method for high-pressure propagation and rapid seedling culture of acanthopanax giraldii harms, and belongs to the technical field of rapid propagation of acanthopanax giraldii harms. The method comprises the following steps: 1-3 days before high-pressure propagation in April to August, shading seed trees of acanthopanax giraldii harms; the method comprises the following steps: selecting branches which grow for more than or equal to 1 year on an acanthopanax giraldii harms seed tree, girdling, and scraping away phloem of the girdled part; carbendazim, a rooting solution 1 and a rooting substrate are mixed and filled into a breeding box, the breeding box is pressed at the branch girdling position, a girdling notch is kept in the dead center of the breeding box, and the breeding box is fixed; the relative humidity of the rooting substrate in the breeding box is kept to be 70%-80%, and a rooting promoting nutrient solution is supplemented regularly. According to the method, the problems of microbial contamination and browning in traditional tissue culture are solved, only 4-6 months are needed, the seedling culture time of the acanthopanax giraldii harms is greatly shortened, and the reproduction rate and the branch utilization rate are remarkably increased; the rooting rate and the survival rate both reach 90% or above, and the material cost and the time cost are saved.

A method for detecting subbark boring pests of trees based on X-rays belongs to the field of non-destructive detection technology. By utilizing the difference in density and thickness between the phloem of trees and the pests, it is possible to distinguish whether there are pests inside the trees, thereby being able to accurately monitor the boring and harmful behavior of the pests inside the trees. The present invention is implemented through the following steps: S1, installing an X-ray imaging detection system; S2, acquiring an image; S3, image processing (grayscale gamma correction); S4, image analysis. The present invention uses X-ray technology to scan trees suspected of being infested by boring pests. It can accurately and efficiently find the location of pest activity under the bark without damaging the growth of the trunk, thereby improving the accuracy of single-tree detection, and does not require a large amount of manpower and material resources. It is efficient and practical, and has a good development prospect.

The invention discloses a method for rapidly propagating populus lanceolata by using winter dormant coppice shoots, and belongs to the technical field of biological agriculture and related industries, forest tree breeding and seedling raising and seed and seedling cultivation. Comprising the following steps: (1) collecting annual coppice shoots of populus lanceolata and annual coppice shoots of populus taishanensis in a dormant state; (2) cutting the lower part of the dormant coppice shoot of the populus lanceolata to form an inclined surface, and splitting the upper part of the dormant coppice shoot of the populus tai from the middle; (3) inserting the cut dormant coppice shoot of the populus lanceolata into a split crack of the dormant coppice shoot of the populus lanceolata, and enabling phloems of the dormant coppice shoot of the populus lanceolata and the dormant coppice shoot of the populus lanceolata to be connected to obtain a populus lanceolata-populus (4) carrying out cleft grafting on coppice shoots of the populus lanceolata and the populus taishanensis and carrying out sand storage; and (5) taking out the sand-stored populus lanceolata-populus tai cleft grafting coppice shoots, and cutting the coppice shoots on outdoor open-air land. According to the method for rapidly propagating the populus sutchuenensis by using the winter dormant coppice shoots, the propagation speed of the populus sutchuenensis is increased, and the growth time of the populus sutchuenensis in the current year of propagation is prolonged.

The invention discloses a tree peripheral bark callus inducer as well as a preparation method and a use method thereof, and belongs to the technical field of plant tissue hyperplasia. The inducer is prepared by mixing the following components in parts by mass: 900 to 1100 parts of water, 90 to 130 parts of a humectant, 0.01 to 0.5 part of a proliferation agent and 0 to 15 parts of a protective agent. When the inducer is prepared, firstly, water is heated to 50-60 DEG C, under the condition of heat preservation, the humectant weighed according to the proportion is added and fully stirred to be dissolved, then the protective agent weighed according to the proportion is added and fully stirred to form a homogeneous system, then the temperature is reduced to the room temperature, the inducer weighed according to the proportion is added, and the homogeneous system is formed after the inducer is fully stirred again. When in use, the inducer is prepared according to the formula proportion and the method; the composition is sprayed on a fresh wound surface of a peeled bark, the dosage is 0.06-0.10 ml / cm < 2 >, and then the wound surface is wrapped by a degradable film. The inducer can effectively shorten the callus time of peridermis, promote the formation of hyperplastic tissues, increase the growth amount of cork and improve the quality uniformity of the cork.

A method of making a tree injection device includes identifying a species specific depth at which phloem is located beneath a tree's bark and preparing a tree injection device. The tree injection device has a head defining an input opening for receiving the solution and a shank. A shank of the device has a radially constricted section positioned between a first radially enlarged section and a second radially enlarged section. An output opening for delivering the solution is defined by the radially constricted section and is formed at a distance from a collar substantially equal to the depth.

The application discloses a method for detoxification of jujube tree stem tips, which comprises the steps of material pre-culture, secondary cutting of stem tips, loading, vitrification of ultra-low temperature treatment, thawing and recovery culture. The application effectively improves the survival rate of stem tip explants by optimizing the pre-culture in the medium of the formula and secondary cutting of stem tips, and to some extent, solves the contradiction among the size of stem tips, survival rate and detoxification rate. The phloem-associated virus is detoxified by the treatment of ultra-low temperature freezing therapy, and the survival rate of the explants reaches 50%, and the detoxification rate is 100%. Therefore, the jujube stem tip ultra-low temperature freezing detoxification technology can effectively remove the phloem-associated virus of winter jujube.

The invention relates to the technical field of carya illinoensis, and particularly discloses a method for controlling vigorous growth of carya illinoensis by inversely cutting the peel of carya illinoensis. The invention relates to a carya illinoensis' inverted bark cutting 'vigorous growth control method, which comprises the following steps: S1, girdling: girdling a vigorous tree trunk or branch of a carya illinoensis tree by using a girdling cutter, cutting off bark and phloem, forming a strip-shaped annular groove in the tree trunk or branch, and retaining the cut annular bark; s2, inversely cutting the barks: taking the cut annular barks, inverting the annular barks, enabling the A ends of the annular barks to face the ground, enabling the B ends of the annular barks to face the crown, and embedding the barks ends of the inverted annular barks into the strip-shaped annular grooves to complete embedding of the inversely cut barks; and S3, post-processing. The method has the advantages of being easy to operate and excellent in vigorous growth control effect.

The invention provides a method for carrying out histological staining by using a protein marker and application of the method in distinguishing sieve molecules and parasporal cells, and belongs to the technical field of biological histology. The invention discloses a method for distinguishing early tubular cells of plant phloem sieve molecules and paragrams, which can distinguish two symbiotic tubular molecules, namely sieve molecules and paragrams, of the phloem by utilizing an apparent protein marker and adopting a histological staining method before cell nucleus of a specific structure such as the sieve molecules disappears by utilizing whether the cells are colored or not; meanwhile, the specific position of interaction between the methylated histone and the paragraph can be distinguished according to the coloring position and the coloring depth, the development degree of the paragraph can be evaluated, and the sensitivity and the precision are high.

The application discloses a method for improving survival rate of palm-leaf wood budding, and belongs to the palm-leaf wood grafting technical field.The grafting method is as follows: (1) selection of stock: selecting palm-leaf wood with a result rate and oil content lower than normal for more than three years as the stock; (2) selection of scion: selecting one-year-old branch of palm-leaf wood with excellent traits and stable yield for more than two years as the scion; (3) grafting: cutting out bud pieces on the scion and waiting for use; cutting out incisions with the same size as the bud pieces on the stock, reaching the phloem, and embedding the bud pieces into the incisions on the stock after taking out, and then binding tightly, so that the problem of low survival rate of palm-leaf wood grafting propagation is solved, and the economic benefit of economic forest is improved.

The invention belongs to the field of molecular biology, and particularly relates to application of an MdHT13.2 gene in regulation and control of plant growth. According to RNA-seq (Ribonucleic Acid-Seq) data analysis, MdHT13.2 with high expression quantity in apple leaves is screened out. Mutant yeast sugar absorption complementation experiments show that MdHT13.2 not only has transport activity on hexose such as glucose, fructose and the like, but also has stronger transport capacity on cane sugar. The results of in-situ hybridization of apple leaves and GUS staining experiments of arabidopsis show that MdHT13.2 has a high expression level in the phloem of the vein. The MdHT13.2 as a member in a hexose transporter gene family can regulate and control sugar accumulation in plants and promote growth of the plants.

The application discloses a method for improving the growth and fruiting rate of 2-3-year-old Carya illinoensis container grafting seedlings after afforestation, which comprises the following steps: selecting 2-3-year-old Carya illinoensis container grafting seedlings and young seedling container seedlings, transplanting the container seedlings and the young seedling container seedlings into planting ponds in an afforestation area, setting up a wound on the stem bark of the container seedlings and the young seedling container seedlings respectively after scratching the stem bark, binding the wounds of the container seedlings and the young seedling container seedlings by using plastic cloth, filling soil, and normally maintaining; after the wounds are fully healed, the plastic cloth is removed, the part above the wound joint of the young seedling container seedlings is cut off, and the young seedling container seedlings are normally maintained until the roots of the young seedling container seedlings and the Carya illinoensis container seedlings are alive. The method can not only protect the root system of the container seedlings by the root system protection matrix, but also can release the root system winding, enhance the root system activity, and promote the rapid growth and development of the root system after transplanting.

The invention discloses a method for improving the salt tolerance of a woody plant through interspecific grafting, in particular to a method for improving the salt tolerance of a scion through grafting by taking a salt-tolerant variety as a stock, and relates to the field of grafting planting. The method comprises the following steps: (1) cutting an annual branch of a salt-tolerant woody plant as a stock, and cutting a phloem at the upper end and the middle part of a cambium from top to bottom; (2) cutting an annual branch of the woody plant and reserving 2-3 buds as scions; (3) aligning and abutting cambiums on the two sides of the long cutting surface of the scion and cambiums on the two sides of the cut of the stock, and binding with a plastic film strip; after the grafting succeeds, one vigorous leaf bud is reserved in the scion. According to the method, the populus nigra scion is grafted to the salt-tolerant American black poplar stock, so that the salt tolerance of the populus nigra scion is improved, the obtained grafted seedling has better growth advantage and salt tolerance, and the method has important application value in increasing the forest yield and cultivating new salt-tolerant varieties.

The invention discloses a traditional Chinese medicine preparation and method for preventing and treating navel orange liberobacter asiaticum. The prevention and treatment method comprises the following steps: a trunk infusion step: infusing a diluent of the traditional Chinese medicine preparation into xylem and / or phloem of a plant trunk; and a foliage spraying step: spraying a foliage fertilizer containing pyroligneous liquor to the foliage of the plant. The traditional Chinese medicine preparation comprises a Chinese herbal medicine extracting solution and aminated xylan. According to the method, a synergistic system of bacteriostasis, nutrition and immunological enhancement is formed through a comprehensive prevention and treatment method of trunk infusion and foliage spraying of the pyroligneous liquid foliage fertilizer. The traditional Chinese medicine preparation infused into the trunk inhibits pathogenic bacteria from the inside in a long-acting mode, the pyroligneous leaf fertilizer provides nutrition from the outside, regulates growth and induces system resistance, internal and external synergism is achieved, diseases are directly inhibited, the nutritional status of diseased trees is remarkably improved, the autoimmunity of the trees is enhanced, and both symptoms and root causes of the huanglongbing are treated.

Disclosed is method for converting a beta-lactam antibiotic into a “masked” beta-lactam antibiotic to permit it to cross the waxy cuticle of a plant and then subsequently unmasking the beta-lactam and converting it into an active beta-lactam antibiotic in the plant phloem. The method permits the use of beta-lactam antibiotics to be used to treat a variety of plant bacterial infections that was not previously possible because the native beta-lactam antibiotics cannot cross the waxy cuticle of plants. In one embodiment the disclosure finds special use in the treatment of bacterial infection of citrus plants with Huanglongbing disease.

The invention belongs to the technical field of insect investigation, and particularly relates to a plug-in type insect trap and an insect trapping method. The plug-in type insect trap is reasonable in design, all parts cooperate with one another, pine branch rods and pine branch leaves are simulated through branch simulants, an atomizer is installed in a supporting rod, and the insect trapping effect is improved. The atomizer can atomize attractant liquid, the attractant liquid is transmitted and attached to pine branches and leaves through the gas path system, the attractant variety can attract adults of monochamus alternatus to come forward, and when the adults fall into the branch simulants sufficiently, the vibration exciter drives the branch simulants to vibrate through the movable plate and the supporting rod, the adults are induced to fall off due to fake death, and the adults fall into the branch simulants. The adults are guided into the adult longhorn beetle collecting pipe through the sliding cover assembly; the trunk simulant in the adult longhorn beetle collecting pipe is used for simulating the phloem and the outer skin of the pine, and a spawning site is provided; pine wood sections can be reserved in the adult monochamus alternatus collecting pipe, twigs of bark of the pine wood sections are reserved to serve as nutrient supplements, and adult monochamus alternatus and worm eggs can be efficiently captured.

The application discloses a cotton topping regulator with the functions of topping control and fruit promotion, and belongs to the technical field of crop plant growth regulators.The application adopts special compound active components and special inactive additive systems, does not add any exogenous hormones, insecticides, fungicides and other auxiliary materials, and constructs a differential target regulation mechanism.The regulator can act on the cotton apical meristem, flower bud differentiation tissue and phloem nutrient transport channel, and simultaneously realizes four functions of apical vigorous shoot inhibition, invalid bud removal, bud and boll shedding inhibition and photosynthetic product directional transport, thereby replacing artificial topping operation.The application can reduce the cotton bud and boll shedding rate by 18.2%-23.7%, increase the per mu seed cotton yield by 12.6%-19.3%, and simultaneously optimize the cotton fiber quality;the preparation has strong storage stability, is suitable for unmanned aerial vehicles, large-scale traction spraying machines and other full-process mechanized plant protection equipment, has a green and waste-free preparation process, meets the development needs of cotton simplified and green cultivation, and has high field popularization value.

The present disclosure relates to a single stranded RNA vector suitable for introducing a therapeutic agent, such as a peptide, a protein or a small RNA, into a host plant. The vector does not encode for any movement protein or coat protein, but is capable of capable of systemic and phloem-limited movement and replication within the host plant.

The utility model discloses an electric girdling device for winter jujube tree branches, which comprises a direct current brushless motor, a rotating output shaft of the direct current brushless motor is connected with one end of a planetary reducer, and the other end of the planetary reducer is connected with a driving bevel gear. The driving bevel gear is in transmission connection with a girdling transmission gear arranged on the periphery of the turnover girdling tool bit through a gear set. The phloem of a winter jujube tree branch is surrounded and stripped through the girdling tool bit driven by the motor, the clamping mechanism can change along with the diameter of the winter jujube tree branch, operation is easy and convenient, the labor intensity of orchard workers is greatly relieved, and working efficiency is improved.

The invention discloses a method for promoting branching of a central trunk of an apple tree in the field of fruit tree cultivation, which comprises the following steps: step 1, according to regional climate, branching is carried out when a central trunk bud of an apple sapling in early spring is selected; step 2, selecting a bud at the middle-upper part of the central trunk, and cutting off phloem and a small amount of xylem of the apple sapling by using a cutter to form a bud notching opening at the upper side of the bud; 3, a shaping bag is prepared, the two ends of the shaping bag are open, and an inclined supporting frame is arranged in the shaping bag; sleeving one end of a shaping bag at the bud at the middle upper part of the central trunk through a first fixing rope, propping one end, close to the bud, of the shaping bag against the bud carving opening, bundling one end, far away from the bud, of the shaping bag through a second fixing rope, and smearing gum on the upper surface of the shaping bag; 4, when the top tips on the central trunks of the apple saplings grow to 3-5 cm, the shaping bags are removed. By the adoption of the technical scheme, branching of the central trunk can be promoted through multiple ways, and the included angle between the branch and the trunk is increased.

The invention discloses application of a PdeAPL protein and a coding gene thereof in regulating and controlling properties of a poplar material. According to the invention, a transgenic poplar overexpressed with PdeAPL and a transgenic poplar with reduced PdeAPL gene expression quantity are constructed. Experiments prove that compared with a control group, the plant height, the number of stem nodes and the length of the stem nodes of the transgenic poplar subjected to overexpression of the PdeAPL are all remarkably increased, and leaves become smaller; and the plant height and the number of stem nodes of the transgenic poplar with reduced PdeAPL gene expression quantity are reduced, the length of the stem nodes is increased, and the leaves are enlarged. In addition, the xylem width of the transgenic poplar with reduced PdeAPL gene expression quantity is reduced, and the areas of xylem conduit cells, xylem fiber cells and phloem cells are increased. The method disclosed by the invention is of great significance to reveal the regulation and control molecular mechanism of the development of the stem vascular of the forest, the tree form of the forest, the growth speed and the improvement of the wood quality, and directionally cultivate the forest with the tree form or the material meeting the actual production requirements.

The invention discloses a promoter with a nucleotide sequence as shown in SEQ ID NO: 1, which can improve the expression level of a target gene in a plant, is used for accurately modifying transporter to regulate and control nutrition distribution and heavy metal resistance at phloem, and can be used for cultivating transgenic plant varieties.