362 results about "Phloem" patented technology

The current invention provides methods to silence insect genes by using unpackaged dsRNA or siRNA, in one embodiment such dsRNA or siRNA is present in plant vascular tissue, preferably phloem, more particularly phloem sap, and the insect is a plant sap-sucking insect. Also provided are DNA sequences which when transcribed yield a double-stranded RNA molecule capable of reducing the expression of an essential gene of a plant sap-sucking insect, methods of using such DNA sequences and plants or plant cells transformed with such DNA sequences. Also provided is the use of cationic oligopeptides that facilitate the entry of dsRNA or siRNA molecules in insect cells, such as plant sap-sucking insect cells.

The invention discloses a new technique for culturing apocarya by seeding and grafting in the current year. The technique comprises the following steps: culturing the apocarya in an improved outdoor sowing method for seedling in the current year; cutting the root when seedlings and seeds are transplanted from a seedbed to a nursery, and building a free stock nursery; selecting the robust apocarya seedlings in current year as the free stock which has leading thread more than or equal to 0.5cm from the seedlings sown in the current year; cutting the part of the free stock on the ground above 25cm in a week before grafting; choosing branches grown in the current year having full bud, short inter-node and rich development from a grain ear picking tree, and carrying out patch budding on the branch on the part of the free stock about 10-15cm on the ground in the solar terms having average daily temperature from 25 to 30 DEG C between August and September; and before wrapping up, tearing down a small phloem from the part under the free stock notch so as to ensure that the bleeding can flow out. By utilizing the technical scheme, the apocarya which is sown in the current year can grow into a tree having the thickness meeting the demand of grafting; and through grafting in the current year, the seedling period is shortened, the tree is dwarf, the juvenile phase is shortened, and the fructification is obtained earlier.

The invention discloses a separation and culture method using ginseng cambium stem cells. The separation and culture method using ginseng cambium stem cells is characterized by comprising the following steps: (1) firstly performing disinfection treatment on the root tubers with the diameter of 2 cm of northeast ginseng by 0.1% mercury bichloride for 10 minutes, and then transversely cutting the northeast ginseng into semicircular slices with the thickness of 0.2 cm and containing periderms, phloems and cambiums; (2) placing the semicircular slices on a WPM (woody plant medium) containing 0.1-4.0 mg / L of picloram and 0.1-4.0 mg / L of 2,4-dichlorphenoxyacetic acid, and culturing at 25 DEG C in the dark; (3) taking out the obviously-multiplied explants of the cambiums after two weeks, separating the cambium stem cells, transferring the cambium stem cells on a subculture medium and culturing, wherein the subculture medium is a WPM containing 0.1-4.0 mg / L of picloram and 0.1-4.0 mg / L of 2,4-dichlorphenoxyacetic acid, and subculture is performed every two weeks. According to the method, separation and culture for ginseng stem cells are performed by using the unsplit cambium stem cells, and the cambium stem cells are infinitely multiplied, so that production can be effectively expanded.

The invention relates to a cutting device of phloem fiber, which is characterized in that the cutting device comprises a reverse U-shaped machine frame, one side of the machine frame is provided with a motor, the motor drives a group of worm and gear mechanisms through a transmission mechanism, worm wheels in the worm and gear mechanisms are coaxially connected with a crank disc of a crank connecting rod mechanism, a connecting rod is eccentricly connected on the crank disc, the other end of the connecting rod is connected with a cutter, the cutter spans between two vertical beams of the machine frame and is movably supported on the cross beam of the machine frame, the cutter is provided with a guide block and a guide groove in inclined movement correspondingly to the cross beams of the machine frame, both sides of the machine frame under the cutter are respectively provided with a material inlet support plate and a material outlet support plate, a cutter falling groove right aligned with the cutter is connected between the material inlet support plate and the material outlet support plate, and a hard pad block is arranged in the cutter falling groove. When the device provided by the invention is used for cutting the phloem fiber, the time and the labor can be saved, the cutting length required by the technology can be realized, the manual operation error can be reduced, and the production efficiency is high.

The invention relates to a mechanical degluing method and a device of phloem fiber. The method of the invention comprises the following steps: 1) spreading and flatly paving phloem fiber in bundles, manually or mechanically feeding the phloem fiber between two rows of upper rollers and lower rollers in arrangement in pairs; 2) driving the upper rollers and the lower rollers to do alternating movement in the positive direction and the reverse direction through a motor, in addition, ensuring that the movement time in the positive direction is longer than the movement time in the reverse direction, and repeatedly extruding, kneading and forwards conveying the phloem fiber; 3) carrying out manual or mechanical oscillation and impurity removal through flapping on colloidal particles and dust on the phloem fiber at the same time of conveying the processed phloem fiber in the second step by a conveying belt; and 4) carrying out manual and mechanical shaking for further impurity removal on the processed phloem fiber in the third step, and then, packing the phloem fiber into bundles. The invention has the advantages of reasonable structural design, ideal degluing effect, small degluing pollution and safe and reliable operation, and can be widely used in the degluing process of phloem fiber, leaf fiber and gramineae fiber raw materials and the like.

The invention discloses a mango grafting cap cut-grafting method which can improve the grafting survival rate, and belongs to the technical field of fruit tree grafting. The mango grafting cap cut-grafting method comprises the steps of slantly downwards cutting from the lower portion of a bud (3) to form a scion slant cutting face (11), then upwards cutting along a forming layer of a scion (1) from a scion tip part (12) to form a scion long cutting face (13) and a cut scion phloem (14), slantly upwards cutting from one side of a root stock (2) to form a root stock slant cutting face (21), then downwards cutting along a forming layer of the root stock (2) from a root stock tip part (22) to form a root stock long cutting face (23) and a cut root stock phloem (24), pasting the scion long cutting face (13) and the root stock long cutting face (23) together in an aligning mode, wherein the cut phloems are pasted to slant cutting faces respectively, and finally conducting tight sealing and binding to finish the grafting process. The mango grafting cap cut-grafting method is simple and practical, high in survival rate and capable of used in popularization and application of mango seedling nursery stock grafting, top grafting seed exchanging, stem cutting seed exchanging and other aspects.

The invention relates to a compound preparation for controlling rubber tree trunk phloem necrosis and a preparation method thereof. The compound preparation consists of anti-oxidation substance and methylcellulose. The mass ratio of the anti-oxidation substance to the methylcellulose is 0.5-3.0: 1, preferably 1.0-2.5: 1, more preferably 1.5-2.0: 1. The preparation method provided by the invention is simple and feasible. The prepared compound preparation can prevent dead bark and eliminate the occurrence of the dead bark, and has remarkable effect.

The invention discloses an ancient tree rejuvenation technology based on inverted bark-whip grafting for ginkgobiloba. According to the invention, a young seedling which is 3-5 years old and is good in growth condition, advanced in root system and free from diseases and pests is taken as a scion, an ancient ginkgobiloba tree which has a rot root system and weak in growth condition is taken as a tree stock, and an inverted bark-whip grafting method is applied to solve the rejuvenation problem of the ancient ginkgobiloba trees which are aged more than one hundred of years, are seriously damaged and have the root systems losing activity basically and solve problems which cannot be solved by using methods such as hole filling, pruning, soil replacement for root parts, fertilization and nutrient solution spraying in an ancient ginkgobiloba protection process. According to the ancient tree rejuvenation technology, the grafting principle is utilized to make phloems and cambiums of the ancient and young ginkgobiloba trees in complete butt-joint, the root system of the young ginkgobiloba seedling is used to replace the death root system of the ancient ginkgobiloba tree to be saved for absorbing nutrition and water so as to meet the growth requirements of the ancient tree, and the ancient tree rejuvenation technology has an obvious effect for the ancient ginkgobiloba trees which are aged more than one hundred of years, are seriously damaged and have the root systems losing activity basically, are simple and convenient to operate and are feasible.

The invention discloses a paraffin slicing method of tree stem tissue, and belongs to the technical field of plant microscopic structure slicing. The paraffin slicing method of the tree stem tissue comprises the operations of tissue fixing, primary softening, dehydration, transparency, waxing, embedding, secondary softening, slicing, slice folding, slice gluing, slicing roasting, dewaxing, rehydration, dyeing, slice sealing and the like, wherein the addition of softening treatment can solve the problem that phloem, a forming layer and xylem in the stem tissue are easy to break in the slicing process due to inconsistent hardnesses. The softening treatment comprises the steps of firstly after the tissue is fixed, adding the operation of using ethyl alcohol / glycerinum mixed liquor to soften the tissue; secondly after embedding and coarse machining, adding the operation of using warm water to soak so as to soften the tissue to be sliced. Experiments demonstrate that a softened tissue can lower probability that the stem tissue is easy to break during slicing, and improve slicing success rate.

The present invention relates to a strain capable of producing bast fiber degumming enzyme and its application for degumming ramie and hemp, belonging to the field of microbial technology. Said strain is bacillus subtilis No.13, and its preservation number is CCTCC, No.M200038. Its degumming rate can be up to 95%, its degumming effect is good, and its degumming process can be completed within 2-6hr., and the fibre separation index is 100%.

The invention discloses a bridging therapeutic method for seriously damaged trees which means that the technical principles of grafting and regeneration are utilized to engraft and communicate with the damaged part caused by insect pest or band girdle of trees, and then the branches or bark of the same trees are taken as a cion to cure the damaged part by means of bridge connection to make the tree grow normally. The method can avoid the abnormal growth or life lost of the grand tree or noble tree caused by the damage of phloem. The method of operation is that firstly, the cion is made ready according to the damaged shape of the tree, and then partial dry and decayed xylem and phloem of the damaged part are taken out to make the interface of the fresh and undamaged xylem and phloem reveal, the edges of the two ends of the cion are cut into a wedge shape dovetailing with the interface and then are closely pressed on the damaged part of the tree; the plastic cloth or parchment paper is used to bind up the damaged part; finally, the ligature is taken out after the callus outgrows and becomes firm. The invention has a survival rate up to as much as more than 95 percent, and has characteristic of simple and convenient manipulation.

The invention provides a seaweed chelate tree nutrient solution and a preparation method thereof. The seaweed chelate tree nutrient solution comprises seaweed extracting solution, sugar alcohol, secondary element of metal salt and microelements. According to the seaweed chelate tree nutrient solution, rich sugar alcohol material in the seaweed is utilized for being combined with various nutrient substances so as to form stable complex, a sugar alcohol complex has low molecular weight and can carry the nutrient and enter phloem for rapidly transporting the nutrient to the part of a crop needed the nutrient. The method can prepare natural nonhazardous tree nutrient solution, improve the survival rate of the transplanted trees, improve the disease resistance of the trees, and further improve the urban landscaping efficiency, and reduce environment contamination.

The invention discloses a grafting method of Magnolia wufengensis. A Magnolia denudate nursery-grown plant is taken as a rootstock, a current-growth branch of the Magnolia wufengensis is taken as a scion, and when the branch has a lignifying tendency in autumn, grafting can be started. The grafting method has the following steps: cutting an angular cut reaching xylem closely at the inner side of a phloem portion, then, performing longitudinal vertical sliding cutting downwards from right above the angular cut to the angular cut so as to cut a flat and smooth long tangent plane and ensure that the exposition position of the long tangent plane is mainly disposed on a cambium layer; performing sliding peeling downwards at the opposite side of a scion axillary bud to form a flat and smooth long-horse-ear-shaped tangent plane, wherein the tangent plane bridges over the diameter of the whole branch, a heart center is seen below the middle of the long-horse-ear-shaped tangent plane, and the axillary bud is sheared at a position 0.8 to 1cm away from the upper portion of the axillary bud; and carrying out scion colligation. According to the grafting method provided by the invention, the grafting butt joint surface is enlarged, the more callus a wound generates, healing can be speeded up, through such a means, the technical difficulty of rapid breeding of the Magnolia denudate is solved, and the technical defect is detected that the survival rate by use of a conventional Magnolia denudate grating method is low.

The invention discloses a salviae miltiorrhizae planting method. The method comprises the steps of field selection and preparation, seedling transplantation, field management and pest and disease prevention. Field management further comprises defloration management and water and fertilizer management. During defloration management, inflorescences of salviae miltiorrhizae are sheared off in the morning, at noon and in the evening in the squaring stage, initial flowering stage and full-bloom stage. During water and fertilizer management, base fertilizer is applied during field preparation, wherein the base fertilizer comprises organic fertilizer 3000-5000 t / hm<2>; 50-80 kg / hm<2> potash fertilizer is applied in the 15-20 leaf stage; 40-60 kg / hm<2> potash fertilizer and 25-35 kg / hm<2> phosphate fertilizer are applied every 30 days in the flowering stage and fruiting stage from June to September. By the adoption of the method, vegetative growth and reproductive growth are strictly controlled in the salviae miltiorrhizae growing process, the probability of root splitting is reduced, growth of the phloem of salviae miltiorrhizae is promoted, accumulation of effective constituents of the roots of salviae miltiorrhizae is improved, and then the rhizome quality of salviae miltiorrhizae is improved.

A trunk ring type trunk cultivation modeling method of ornamental trees is characterized by comprising the following steps that in the early spring, after the soil is unfrozen and when the sap flows, trees are selected for standby application; the trees with different determined trunk heights are combined and planted together, the highest one is selected and cultivated as the main trunk and used for the sheared modeling at the highest layer, and the others are mutually bent in the direction perpendicular to the main trunk, serve as lateral brunches to be cultivated and are used for modeling at other layers; the trunk modeling is combined according to the classification of an annular trunk modeling, the trunk modeling is decomposed to obtain a single-tree technical graph, the shape is determined depending on the trees, the height positions of the highest and lowest trunks and the trunks at the front, back, left and right sides of the whole tree are determined, and unnecessary brunches are removed; the contacting surfaces between the trunks in the planting process are cut to the phloems through a grafting tool and used for inarching, and the outside is wrapped and bound tightly through a plastic thin film with the thickness of 0.02-0.06 mm; a supporting frame and a ring type trunk cultivation modeling ladder frame are manufactured and installed; in the growth period and after the inarching surfaces are healed, the plastic thin film is untied.

The invention discloses a multilayer structure electrolytic capacitor paper and a preparation method of the electrolytic capacitor paper. The multilayer structure electrolytic capacitor paper consists of voltage withstanding layer paper and absorption layer paper. The paper pulp of the voltage withstanding layer paper is selected from Agave sisalana, and the beating degree of the paper pulp employed by the voltage withstanding layer paper is 32-38degree SR. The absorption layer paper is a one layer or multilayer structure, the paper pulp of the absorption layer paper is one of or a mixture of several of bast fiber, phloem fiber and grass fiber, and the beating degree of the paper pulp employed by the absorption layer paper is 15degree SR-45degree SR. The preparation method of the electrolytic capacitor paper includes the preparation method of the voltage withstanding layer paper and the absorption layer paper. The method consists of: beating, net paper making, molding of the absorption layer paper and the voltage withstanding layer paper on net respectively, then compounding, and drying and reeling. The electrolytic capacitor paper designed by the invention can realize serialized voltage withstanding, and has good voltage withstanding performance and low short circuit rate. The designed preparation method can effectively produce the multilayer structure electrolytic capacitor paper, and avoids defective products in the production process.

The invention provides microbial compound bacteria, a preparation method thereof and application of the microbial compound bacteria to prevention and treatment of citrusyellowshoot. The microbial compound bacteria comprise Cribben paenibacillus and bacillus subtilis, the biological preservation serial number of the Cribben paenibacillus is CGMCC NO.7996, and the microbial compound bacteria can be specifically microbial compound bacterium liquid or a microbial compound bacterium agent. The preparation method of the microbial compound bacteria includes the specific steps that the Cribben paenibacillus and the bacillus subtilis are mixed in proportion after being fermented. According to the microbial compound bacteria for preventing and treating citrusyellowshoot and the preparation method and application thereof, procreation and propagation of gram negative bacteria are inhibited in a root filling and leaf surface spraying mode, 'bacteria are controlled through bacteria', harmful bacteria in soil are killed, soil quality is optimized, root and stem phloem sieve tubes can be dredged, the problem of nutrient substance conveying barrier is solved, diseased or infected plant nutrients are recovered, growth of new roots is promoted, tree roots are more developed, tree tubular cells are smoother, yellow leaves of fruit trees are turned green, and healthy new tips grow.

The invention relates to a termite attractant comprising the following components by weight percent: (1) 60%-85% of ground material of eucalyptus; ground material of camphor tree or mixture of the two ground materials; (2) 3%-9% of sugars; (3) 11.97%-30.95% of flour; (4) 0.03%-0.05% of preservative. ground material of eucalyptus and ground material of camphor tree come from root, trunk phloem or mixture of root and trunk phloem. The preservative uses sodium dehydroacetate or sodium benzoate. The termite attractant is prepared by mixing the ground material of eucalyptus, ground material of camphor tree or mixture of the two ground materials as main material together with flour and sugars. Cooperating with technical product used for killing termite, the attractant is environmental friendly. It is proved by repeated tests that the attractant effect of attracting termite is very ideal when cooperating with technical product and thereby the attractant can be popularized.

The invention belongs to the field of phloem fiber resource utilization, in particular relates to a method for preparing high-quality paper pulp such as rice paper and the like by steam-explosion, degumming and cleaning of broussonetia papyrifera bark. The method comprises the steps as follows: firstly, the steam-explosion treatment of the broussonetia papyrifera bark is carried out; subsequently, rinsing and bleaching are carried out, thus obtaining the high-quality paper pulp. The no-pollution steam-explosion treatment degrades 80% of the pectin and hemicellulose and part of the lignin in the broussonetia papyrifera bark fiber, completely separates the external epidermis (black skin) of the broussonetia papyrifera from the internal layer phloem thereof simultaneously, and removes the black tissue of the external epidermis by the bleaching; furthermore, during the steam-explosion process, the bark fiber is torn to a loose structure, which leads the chemical drug to sufficiently permeate during the bleaching process and improves the whiteness of the paper pulp. The method reduces the industrial cost of the papermaking by broussonetia papyrifera bark, avoids the environmental pollution caused by chemical pulp preparation process and can improve the yield and quality of the paper pulp; and the paper pulp can be used for preparing high-quality paper such as rice paper, bank paper and the like. The invention provides a new method for clean high-value utilization of the broussonetia papyrifera bark resource and generates greater economic and social benefits.

The invention relates to an asexual propagation method for afforestation trees. The method is characterized by comprising the following steps: during the growth stage of the afforestation trees, circularly cutting the bast part of an afforestation tree on the position which needs to root; covering the circular cut position of the tree taking with a nutrition medium used for overhead propagation by layering to serve as filler; covering by a common plastic film; growing to form callus; after root points and fibril grow, cutting a trunk from the callus or the lower end of the fibril; trimming the branches of the cut trunk; covering the trunk and the branches with a colorless transparent plastic film; and transplanting to a destination. The method is suitable for arbor and dungarunga plants, and only the trunk and the branches need to be transported in the transportation process so as to greatly lower transportation difficulty and transportation cost. In addition, the biodiversity of afforestation tree native lands is fully kept, and water and soil loss is prevented. The afforestation trees propagated with the asexual propagation method for afforestation trees is characterized in that the survival rate for transplantation is 90-95% which is 30% higher than that of the traditional soil ball transportation method, and a great quantity of social resource is saved.

The present invention relates to cotton tissue specific and pathogenic bacterium inducing promoter and their application, and belongs to the field of biotechnology. The promoter contains CAAT-box, TATA-box, ethylene, methyl jasmonate, abscisic acid response element, pathogenic bacterium inducer response elements W boxes, GT-1, MYB, MYBST1, MYB1LEPR, etc. There are also specific root expressing elements to enhance the expression of GUS gene, which expresses specifically in the phloem of root, bud and stem, the vein of foliage and the guard cell of air pore. Paraffin section observation shows that GHNBS promoter expresses in phloem companion cell. After treatment with SA, MeJA, Ethylene, pathogenic bacteria of blight and pseudomonas syringae DC3000, GUS will have obviously raised activity, so that it is one organ specific and pathogenic bacterium relevant promoter.

The invention discloses a nursery stock grafting method which comprises the following steps of (1) selection of a stock, selection of a scion, (3) combination, (4) treatment of the combined portion and (5) timely watering after combination. In the combination process, the combined part of the stock is cut open to form a 30-degree oblique plane through a disinfected knife, the phloem of the lower portion of the scion is cut off by 1 cm to 1.2 cm, so that the cambium is exposed, the cambium of the stock is combined with the cambium of the scion. The grafted nursery stock is managed in a conventional method. According to the nursery stock grafting method, the combination area between the cambium of the stock and the cambium of the scion is enlarged, combination is tighter, the grafting survival rate can be increased, the survival rate can achieve 80 percent to 90 percent, the germination rate of the nursery stock after grafting can be increased at the same time, and the nursery stock after grafting can survive ahead of time by 10 days to 15 days.

The invention relates to a method for cultivating citrus germplasm infected with citrus vein phloem degeneration. The method comprises the steps of cultivation and heat treatment of rootstock, collecting of scions, treating drugs, grafting, managing and detecting. According to the method, heat treatment is conducted on rootstock seedlings, the rootstock without the citrus vein phloem degeneration is cultivated, drug treatment is conducted on citrus branches which are infected with the citrus vein phloem degeneration while still have vitality, and the scions are taken from the citrus branches after drug treatment and grafted on the rootstock without the citrus vein phloem degeneration. Samples are collected in four months, eight months and twelfth months after grafted seedlings are survived, and PCR detection and morbidity situation survey are conducted. The result shows that pathogenic bacteria of the citrus vein phloem degeneration of infected scions of citrus elite germplasm or variety can be completely eliminated, the recovery and cultivation effect of infected germplasm resources can be visually and accurately evaluated, and the citrus germplasm resources can be quickly protected and cultivated.

The invention discloses an ointment for preventing and treating insect pest longicorn of a poplar, belonging to the field of pesticide and preparation thereof. The ointment is prepared from the following raw materials by weight percent: 1-10% of Chlorantraniliprole, 1-50% of dichlorvos and the balance of an addition agent. The bark on the trunk of the poplar, with perpendicular distance of 0.3-1.8m from the root of the poplar, is cut off from top to bottom in a spiral ring manner, so as to form a spiral groove, the width of the spiral groove is 2-20cm, the depth of the spiral groove is the distance between the surface of the poplar to the phloem thereof, and the ointment is uniformly spread in the spiral groove. The ointment spread on the part of which the bark is peeled, on the trunk can be absorbed inside through the plant, thus having good adhesive force and long effect, and the ointment is convenient and simple to apply, improves the use ratio, lowers the environment pollution, has certain touch out action, and can prevent pests from creeping on the trunk up and down, thus greatly relieving the harm of the pests to the plant.

The invention relates to a musa fiber, which is prepared from stems of musa plants, which are used as raw materials, wherein the musa fiber comprises the following components: cellulose is 81-95 percent, hemicellulose is 1.0-5.0 percent, lignin is 1.0-2.8 percent, other components are pectin water soluble matter and is based on the dry weight of the musa fiber. The above musa fiber is prepared through the mechanical treatment, acid preimpregnation, washing, soda boil, washing, dehydration and oil supply, the above musa fiber is used to be spun into 10-60 single yarn, the process has low cost, compared with ancient process, the strength and the fineness of the made fiber are improved, and the process of preparing the musa fiber of the invention is also capable of being used to made mulberry fiber. The invention opens a new way for the development and utilization of phloem resources, has an important significance at the present that a part of prior fiber resources are run out, and accords with strategic demands of sustainable development.

The invention discloses a method for extracting total plant proteins, in particular to a method for extracting the total proteins in the phloem of tomato plants. The method includes the following steps: (1) adding crosslinked polyvinylpyrrolidone (PVPP) and quartz sand in plant materials, and grinding the mixture into powder through liquid nitrogen; (2) adding protein extraction buffer solution into the powder, oscillating to homogenate the slurry, and then adding Tris saturated phenol for extracting to obtain an organic phase; (3) evenly mixing the organic phase with the protein sedimentation liquid, standing, and centrifuging to collect the sediments; and (4) sequentially performing methanol cleaning, acetone cleaning, freezing and drying on the sediments to obtain the total plant proteins. The main improvement of the method for extracting total plant proteins is that: (1) the PVPP is added during the grinding and smashing of samples to absorb phenolic substances, and the PVPP can be removed through centrifugal after the phenolic substances are adsorbed, so that the downstream analysis process cannot be affected; and (2) a plurality of protein enzymes are added during the extracting, so that the activity and natural characteristics of the proteins in the plant materials can be maintained to the largest extent.

The invention discloses a citrus vein phloem degeneration detection and classification method based on the D-S theory through multi-source data fusion. The method includes utilizing a hyperspectral image collecting platform, a visible spectrum image collecting platform and a fluorescence spectra image collecting platform to collect image information of citrus leaf samples, performing classification of the symptoms of the citrus vein phloem degeneration on the various spectrum collected image information, and acquiring the identification rate of each classification of the symptoms of three varieties of spectrum; allowing the identification rates of three varieties of spectrum to serve as evidence sources, allocating PBA values to each proposition of an evidence identifying frame, and establishing credibility vectors of the evidence sources; allowing the credibility to serve as a discount factor to adjust the BPA values to each proposition of the evidence identifying frame; fusing the adjusted BPA values according to a Dempster combination rule, acquiring a comprehensive identification rate of the symptoms of the citrus vein phloem degeneration, and implementing correct diagnosis of the symptoms of the citrus vein phloem degeneration. According to the method, the identification rates of the hyperspectrum, visible spectrum and fluorescence spectra are fused, the comprehensive identification rate of the symptoms of the citrus vein phloem degeneration is acquired, and the correct diagnosis of the symptoms of the citrus vein phloem degeneration is implemented.

The invention discloses a method for treating the citrus yellow shoot. Particularly, the method includes the following steps of 1, the soil sterilization process; 2, the soil improvement process; 3, the process of treating root systems of diseased plants; 4, the nutrition supplementing process conducted after the plants are cured. According to the method, the citrus yellow shoot in rutaceous plants (especially rutaceous fruit trees) can be effectively treated, and prevention and treatment effects on the aspects of soil bad bacterium treatment, plant root system phloem bacillus treatment, plant nutrition supplementing, maintenance after cure and the like are provided. The whole technology is complete, effective and safe, organic biological material products used in the treatment process are all safe and free of toxins, no other pesticide needs to be used, the production cost is reduced, harvested fruits can achieve the purpose of zero pesticide residue, the freshness and taste are kept, and the yield of the fruits is increased.

The invention discloses a novel method for degumming a paper mulberry bast fiber for pulping, which performs degumming to aqueous solution of the paper mulberry bast fiber for pulping at a temperature of 30 to 60 DEG C and by pectinase; the pH value of the aqueous solution of the paper mulberry bast fiber is 5 to 9. The degumming method provided by the invention fundamentally eliminates the pollution problem generated by a chemical degumming method, has no chemical residue in the waste water negated by degumming, and has no pollution to the environment; in addition, the utilization rate of the raw materials is increased by 5 percent compared with the prior chemical method, the fiber raw material used for paper making and obtained after the degumming fully meets the requirement of pulp paper making, and the obtaining rate is above 70 percent and is increased by 15 percent compared with the prior art. The method realizes that the environmental pollution while increasing the utilization rate of resource is reduced. The method has wide application prospect.

The invention provides a method for identifying pathogen of the paroxysmal foliage wilting disease in myrica rubra, comprising separating the xylem from the phloem of a myrica rubra branch, respectively cutting the phloem and xylem into small sheets of tissue without the surface disinfection, placing them on a PDA flat, culturing in a thermotank of 25 DEG C for 2 days, picking out fluffy fungal hyphae and placing the fungal hyphae to another new PDA flat, continuously culturing in the thermotank of 25 DEG C for 5 days, and picking out purified hypha blocks of 0.5cm<2> for the inoculation experiment of the pathogenicity. The method for testing the pathogen of the paroxysmal foliage wilting disease in myrica rubra in vitro is reliable and fast, needs no expensive materials, comprises simplesteps, and has high accuracy.