19 results about "Budding" patented technology

The invention discloses a compound microbial agent for efficiently promoting attachment and metamorphosis of coral larvae and growth of the larvae and application of the compound microbial agent. The microbial agent comprises two strains, namely, Phytobacter sp.SCSIO43973 and Salipiger sp.SCSIO43974, and the preservation numbers of the two strains are respectively as follows: GDMCC (China General Microbiological Culture Collection Center) NO: 65583 and 65650. The two strains are separated from healthy coral deerhorn-shaped corals. Researches show that by adding the strains, the adhesion rate and the metamorphosis rate of planula larvae and the survival rate and the germination reproduction rate of coral larvae can be remarkably increased. The characteristics show that the microbial agent has the potential of improving the attachment metamorphosis rate of coral larvae and promoting growth in a field coral reef ecological system, and has important significance in increasing the larva supplement amount of the damaged coral reef ecological system and promoting the recovery and sustainable development of the coral reef ecological system. In conclusion, the microbial agent provided by the invention provides a new technical means for recovery of a coral reef ecosystem by promoting attachment and metamorphosis of coral larvae and growth of the larvae.

The present invention discloses a composite microbial agent for degrading diazepam and its application. It belongs to the field of biodegradation technology. The composite microbial agent comprises the following components: Bacillus subtilis, yeast, Cladosporium cladosporium, Aspergillus oryzae and Aspergillus niger. The present invention prepares a composite microbial agent by screening suitable microbial strains and applies it to the degradation of DZP in aquaculture ponds. In terms of degradation rate index, the plant remediation method can reach up to about 70%, the nano zero-valent copper method can reach up to about 75%, and the degradation rate of the technical solution of the present invention can reach up to 100%. Comprehensive comparison shows that the technical solution of the present invention shows great superiority in degrading DZP residues in aquaculture water bodies and has significant promotion and demonstration prospects. At present, there is no report on the degradation of DZP in aquaculture pond water bodies by microbial methods.

The invention relates to the technical field of functional molecular markers, in particular to a DNA (deoxyribonucleic acid) molecular marker related to rice germination stage flooding-resistant seedling gene haplotype. The haplotype of the waterflooding-resistant seedling-forming gene OsTPP7 in the bud stage of the rice consists of SNP282722617, SNP282723164, SNP282723639, SNP282723858 and SNP282727202, and the haplotype of the waterflooding-resistant seedling-forming gene OsTPP7 in the bud stage of the rice consists of SNP282722617 When a corresponding basic group of the five SNPs is ACGCA, the five SNPs are favorable haplotype (Hap7) of the OsTPP7 gene, and the flooding resistance in the bud stage is high. The molecular marker provided by the invention can be used for haplotype analysis of the OsTPP7 gene with the flooding-resistant seedling-forming ability in the bud stage of different rice materials, breeding materials with the flooding-resistant ability in the bud stage of the rice are screened through the molecular marker, and compared with traditional phenotype screening, time and labor are saved, efficiency is high, and cost is saved; the method can be applied to molecular breeding, and the breeding efficiency is improved.

The invention relates to the technical field of biology, in particular to a tremella aurantialba strain DZHSSJE001 and an industrial cultivation method thereof, the tremella aurantialba strain DZHSSJE001 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.41289, the ITS sequence is shown as SEQ NO.1, the tremella aurantialba strain is in a yeast state in a culture state, conidia are oval, most of the conidia are top bud reproduction, 1.6-2.7 * 3.4-4.1 [mu] m, and the preservation number is CGMCC NO.41289. Meanwhile, the invention further discloses an industrial cultivation method of the tremella aurantialba strain DZHSSJE001, and the problem that the industrial cultivation yield of the tremella aurantialba strain is poor is solved.

The invention discloses a stereoscopic model for in-vitro culture of zebra fish intestinal organs and a preparation method thereof, and belongs to the technical field of aquaculture organs. The preparation method comprises the following steps: putting a zebrafish intestinal tract sample into PBS containing five antibodies, separating and cleaning to obtain intestinal tract tissues; carrying out vortex oscillation on intestinal tissues in the separation liquid, discarding the separation liquid, carrying out vortex oscillation in the chelating liquid, and filtering to obtain zebra fish crypt cell suspension; carrying out differential centrifugation on the cell suspension, and resuspending the precipitate in a DMEM culture medium to obtain an intestinal organ crypt suspension; the method comprises the following steps: inoculating crypt suspension into a mixed solution of a zebra fish intestinal organ culture medium and matrigel for in-vitro culture to obtain the zebra fish gut organ culture medium. The method solves the problem that the existing method for preparing the zebra fish intestinal organ in-vitro model with high budding rate and good effect lacks, provides a research tool for molecular mechanism research, disease simulation, drug effect screening and the like of intestinal diseases based on zebra fish intestinal organs, and has good application value.

This invention discloses an InDel marker, Pmct3, for cold tolerance during rice budding, along with its identification method and applications, belonging to the field of molecular marker technology. This invention develops an InDel molecular marker from the insertion / deletion sequence between the Dongxiang wild rice introgression line D2 (background of the cold-tolerant rice 9311) and the cold-sensitive rice 9311 at the Chr1:28249918 and Chr1:28249919 sites in the O. sativa reference genome. Primers are then developed based on this molecular marker for identifying cold tolerance during rice budding. The detection cycle of this invention is only 2-3 hours, does not rely on subjective experience in traditional detection methods, and can obtain accurate and objective detection and identification results in a short time. It is low-cost, high-throughput, and highly specific, making it suitable for rice breeding and production practices.

The invention discloses a villous amomum fruit seedling tissue culture rapid propagation method, which is characterized in that villous amomum fruit seeds are used as a material, MS (Murashige and Skoog) is used as a basic culture medium, growth hormones with different proportions are added, adventitious buds are induced to proliferate and propagate, a rapid propagation technical system of villous amomum fruit is established, and after kinetin is added into the induction and proliferation culture medium, the germination speed is high, the germination rate is high, the buds are robust, and the proliferation coefficient is high; after the indoleacetic acid is added into the rooting culture medium, the rooting is fast, the rooting rate is high, the roots are many, the stems are thick, the leaf emergence is fast, the leaves are fleshy and good in growth vigor, and the transplanting survival rate is greatly improved. According to the method, the problems that the fructus amomi seeds are not easy to store and the propagation of the fructus amomi is influenced are solved, the problems that the division propagation rate of the fructus amomi is low and large-scale planting is limited are solved, large-scale propagation of the fructus amomi can be carried out in a short time through the method, and the production and planting requirements are met.

The invention discloses an agrobacterium tumefaciens-mediated soybean sprout tip transformation method. The method comprises the following steps of explant infection and co-culture, bud induction and screening culture, elongation recovery and rooting culture and transgenic plant domestication culture. According to the method, the soybean sprout tips are selected as the explants, the soybean genetic transformation process is simplified, direct budding of apical meristem and synchronous rooting of underground parts can be achieved, only one month is needed from explant infection to obtaining of a complete regenerated transgenic plant with a developed root system, the transformation efficiency is high, the soybean transformation period is greatly shortened, and the method is suitable for large-scale popularization and application. The cost is effectively reduced. Meanwhile, the problem of soybean genotype limitation and the problem that northeast varieties bloom in advance in the tissue culture stage are solved, the method is applied to more than twenty main cultivated varieties difficult to transform, the transformation efficiency is high and stable, the highest efficiency can reach 81%, and reliable technical support is provided for soybean basic research and gene editing breeding work.

The invention discloses a cultivation method and a cultivation evaluation method of Amomum villosum stem tiller seedlings, and relates to the field of cultivation evaluation methods. The cultivation method comprises the following steps: adding 6-benzyladenine into a culture medium to obtain an induced budding culture medium; a tissue culture rapid propagation technology is adopted, leaves and sheaths are cut off, and stems with roots are left; and vertically inserting the stem segments into an induction budding culture medium, and culturing to obtain stem tillering seedlings. The rapid tissue propagation technology is adopted, pathogens and other viruses in the plant body can be effectively removed, the 6-benzyladenine with the high concentration is adopted to induce budding of stem segments, meanwhile, the vertical inoculation mode is combined, budding and proliferation of stem tillering seedlings are effectively improved, the seedling growing period is short, high-speed and high-yield high-quality seedlings can be achieved, and the method is suitable for large-scale popularization and application. And excellent germplasm resources required by industrial development are ensured.

This invention discloses the application of the rice OsCK1a.1 gene in regulating seed dormancy and panicle budding resistance, belonging to the field of plant genetic engineering technology. To achieve panicle budding resistance breeding in rice, this invention targets the OsCK1a.1 gene, utilizes CRISPR / Cas9 gene editing technology to construct a knockout vector for this gene, and successfully transforms it into rice, obtaining homozygous mutant plants with the OsCK1a.1 gene knockout. Experimental results show that, compared with the control, homozygous mutants of different mutation types exhibit significantly enhanced seed dormancy levels and substantially improved panicle budding resistance. Therefore, this invention, by knocking out the OsCK1a.1 gene, provides an effective method for creating new rice varieties resistant to panicle budding, which has significant application value for ensuring high and stable rice yields.

The application belongs to the technical field of jellyfish prevention and control, and discloses application of DMX-5804 in preparation of a medicine for inhibiting budding reproduction of Aurelia sp.1. By inhibiting budding reproduction of A.coerulea hydrocladium, the outbreak of Aurelia sp.1 can be prevented and controlled, and the adverse effects on marine ecological environment and human coastal economic activities can be reduced. Through experimental verification, 1 μM DMX-5804 can reduce the number of hydrocladium buds without affecting the living state of A.coerulea hydrocladium, which indicates that the compound DMX-5804 can inhibit the asexual reproduction of Aurelia sp.1 without toxicity at this concentration. The application provides a new theory and method for controlling the number of jellyfish, preventing the outbreak of Aurelia sp.1, and studying the biological conservative cell proliferation strategy.

The invention provides a bud grafting-based rubber tree breeding new material induction method. The method comprises the following steps: S1, selecting a stock variety and a scion variety; s2, the rootstock variety and the scion variety are rejuvenized; s3, stock and scion preparation; s4, budding; s5, screening of bud grafting mutagenesis materials: phenotype identification screening, stress resistance identification screening or molecular identification screening; according to the method, rubber tree varieties with complementary characters are selected as stocks and scions, so that the overall characters are effectively improved; target traits of the scions are gradually induced to be effectively optimized through multiple budding of the young budlings, and the stability of the traits is improved; through molecular identification screening, adversity stress screening and phenotype identification screening, a target budding mutagenesis plant is rapidly and accurately screened out, the new variety breeding process is accelerated, the breeding period is shortened, and the idea is broadened for creation of new rubber tree breeding materials.

The invention discloses an application of capsaicin in intestinal organ culture and a culture solution containing capsaicin, and belongs to the technical field of tissue culture. The intestinal organ culture solution comprises capsaicin with the concentration of 1.25-5 [mu] mol / L and a stem cell culture solution. When the culture solution is used in pig jejunum organ culture, generation and budding of jejunum crypt organs can be remarkably promoted, and cell growth and intestinal organ amplification are effectively promoted. According to the method for promoting amplification of the intestinal organs provided by the invention, many limitations of a traditional model can be overcome, and the development of intestinal pathology is assisted.

The present invention relates to the field of viral vectors, and in particular to a fusion polypeptide and a use thereof. Disclosed are a fusion polypeptide and a use thereof. The fusion polypeptide can mediate the budding of a pseudotyped LVV or RVV in packaging cells. Also disclosed is a particle comprising the fusion polypeptide, wherein the non-specific transduction capability of the particle is reduced.

The present application belongs to the technical field of plant breeding, and particularly relates to a method for breeding alfalfa. The method comprises the following steps: 1) land preparation and seeding; 2) parent alfalfa growth management; 3) promoting the present budding and flowering of the parent alfalfa (accelerating the conversion of vegetative growth to reproductive growth) and pollination; 4) promoting seed plumping and maturation; 5) seed harvesting and seed treatment; 6) land preparation and summer seeding of the first generation seeds; 7) summer growth and development management of the first generation alfalfa; 8) present budding, pollination and seed harvesting of the first generation alfalfa; 9) autumn seeding and winter protection of the second generation alfalfa seeds. The method can significantly shorten the alfalfa breeding cycle, effectively solve the problem of low alfalfa breeding efficiency in China, and perfect the grass breeding technology system in China, which has far-reaching significance for the development of grass and animal husbandry, food security and ecological environment protection in Henan and even in China.

The present application relates to the tissue culture technical field of Yunnan rhizoma paridis, and particularly relates to a method for establishing a high-efficiency tissue culture seedling system of Yunnan rhizoma paridis, comprising the following steps: S1. inducing Yunnan rhizoma paridis secondary buds; S2. activating the budding of Yunnan rhizoma paridis secondary buds; S3. growing Yunnan rhizoma paridis seedlings; S4. transplanting Yunnan rhizoma paridis seedlings; S5. repeating steps S3-S4 to grow and transplant seedlings for multiple times to obtain perennial seedlings of Yunnan rhizoma paridis. The present application overcomes the problem of "false seeds" of Yunnan rhizoma paridis, prevents the waste of a large number of seedlings, and first establishes a high-efficiency tissue culture system of Yunnan rhizoma paridis. The perennial tissue culture seedlings obtained through secondary bud induction, transplanting and re-induction have biological significance, a large number of Yunnan rhizoma paridis species resources are obtained, and the dilemma of the scarcity of Yunnan rhizoma paridis resources is effectively solved.

A method for high-efficiency regeneration of a potato protoplast is provided, including the following steps: S1, conducting test-tube seedling cultivation; S2, conducting protoplast preparation; S3, conducting callus proliferation; S4, conducting callus synchronization; and S5, conducting callus induction regeneration. Protoplasts derived from treated potato leaves are induced to form a callus. Through callus synchronization and callus induction regeneration, high-efficiency and synchronized budding is achieved, significantly shortening the regeneration timeline of protoplasts and markedly enhancing the bud regeneration rate. This method provides abundant experimental materials for potato genetic improvement and cultivar development, and ensures consistency and stability of regenerated plantlets. This method also offers technical support for downstream applications, including: genetic theory research, high-throughput virus-free plantlet regeneration, and gene-editing breeding in potatoes.

This invention discloses an InDel marker, Pmct1, for cold tolerance during rice budding, along with its identification method and applications, belonging to the field of molecular marker technology. This invention develops an InDel molecular marker by combining the insertion / deletion sequences of the Dongxiang wild rice introgression line D2 (background of the cold-tolerant rice 9311) and the cold-sensitive rice 9311 at the Chr1:32106831..32106871 locus in the O. sativa reference genome. Primers are then developed based on this molecular marker for identifying cold tolerance during rice budding. The detection cycle of this invention is only 2-3 hours, does not rely on subjective experience in traditional detection methods, and can obtain accurate and objective detection and identification results in a short time. It is low-cost, high-throughput, and highly specific, making it suitable for rice breeding and production practices.

The invention discloses application of PSK to improvement of asexual propagation efficiency of lily scales, belongs to the technical field of plant asexual propagation, and particularly aims to solve the problems that existing lily scale cutting propagation is low in efficiency, slow in budding and rooting and the like. The PSK with the effective concentration (preferably 10 nM <-1 > mu M) is prepared into a treatment solution; during cuttage, the water is applied as first-time irrigation liquid, and the physiological response of the scales can be systematically regulated and controlled. Experiments show that the application can significantly improve the budding rate of longya lilium scales, promote the expansion of new bulblets, and synchronously improve the rooting rate. The invention provides a novel efficient asexual propagation method for lily, which is simple and convenient to operate, remarkable in effect and safe, and is suitable for large-scale production of lily seedlings.