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Budding is a type of asexual reproduction in which a new organism develops from an outgrowth or bud due to cell division at one particular site. The small bulb-like projection coming out from the yeast cell is called a bud. The new organism remains attached as it grows, separating from the parent organism only when it is mature, leaving behind scar tissue. Since the reproduction is asexual, the newly created organism is a clone and is genetically identical to the parent organism.
Application of rice OsCK1a.1 gene in regulating seed dormancy and resistance to panicle emergence
This invention discloses the application of the rice OsCK1a.1 gene in regulating seed dormancy and panicle budding resistance, belonging to the field of plant genetic engineering technology. To achieve panicle budding resistance breeding in rice, this invention targets the OsCK1a.1 gene, utilizes CRISPR / Cas9 gene editing technology to construct a knockout vector for this gene, and successfully transforms it into rice, obtaining homozygous mutant plants with the OsCK1a.1 gene knockout. Experimental results show that, compared with the control, homozygous mutants of different mutation types exhibit significantly enhanced seed dormancy levels and substantially improved panicle budding resistance. Therefore, this invention, by knocking out the OsCK1a.1 gene, provides an effective method for creating new rice varieties resistant to panicle budding, which has significant application value for ensuring high and stable rice yields.
Owner:HUAIYIN TEACHERS COLLEGE