11 results about "Budding" patented technology

This invention discloses an InDel marker, Pmct3, for cold tolerance during rice budding, along with its identification method and applications, belonging to the field of molecular marker technology. This invention develops an InDel molecular marker from the insertion / deletion sequence between the Dongxiang wild rice introgression line D2 (background of the cold-tolerant rice 9311) and the cold-sensitive rice 9311 at the Chr1:28249918 and Chr1:28249919 sites in the O. sativa reference genome. Primers are then developed based on this molecular marker for identifying cold tolerance during rice budding. The detection cycle of this invention is only 2-3 hours, does not rely on subjective experience in traditional detection methods, and can obtain accurate and objective detection and identification results in a short time. It is low-cost, high-throughput, and highly specific, making it suitable for rice breeding and production practices.

The invention discloses a villous amomum fruit seedling tissue culture rapid propagation method, which is characterized in that villous amomum fruit seeds are used as a material, MS (Murashige and Skoog) is used as a basic culture medium, growth hormones with different proportions are added, adventitious buds are induced to proliferate and propagate, a rapid propagation technical system of villous amomum fruit is established, and after kinetin is added into the induction and proliferation culture medium, the germination speed is high, the germination rate is high, the buds are robust, and the proliferation coefficient is high; after the indoleacetic acid is added into the rooting culture medium, the rooting is fast, the rooting rate is high, the roots are many, the stems are thick, the leaf emergence is fast, the leaves are fleshy and good in growth vigor, and the transplanting survival rate is greatly improved. According to the method, the problems that the fructus amomi seeds are not easy to store and the propagation of the fructus amomi is influenced are solved, the problems that the division propagation rate of the fructus amomi is low and large-scale planting is limited are solved, large-scale propagation of the fructus amomi can be carried out in a short time through the method, and the production and planting requirements are met.

The invention discloses an agrobacterium tumefaciens-mediated soybean sprout tip transformation method. The method comprises the following steps of explant infection and co-culture, bud induction and screening culture, elongation recovery and rooting culture and transgenic plant domestication culture. According to the method, the soybean sprout tips are selected as the explants, the soybean genetic transformation process is simplified, direct budding of apical meristem and synchronous rooting of underground parts can be achieved, only one month is needed from explant infection to obtaining of a complete regenerated transgenic plant with a developed root system, the transformation efficiency is high, the soybean transformation period is greatly shortened, and the method is suitable for large-scale popularization and application. The cost is effectively reduced. Meanwhile, the problem of soybean genotype limitation and the problem that northeast varieties bloom in advance in the tissue culture stage are solved, the method is applied to more than twenty main cultivated varieties difficult to transform, the transformation efficiency is high and stable, the highest efficiency can reach 81%, and reliable technical support is provided for soybean basic research and gene editing breeding work.

This invention discloses the application of the rice OsCK1a.1 gene in regulating seed dormancy and panicle budding resistance, belonging to the field of plant genetic engineering technology. To achieve panicle budding resistance breeding in rice, this invention targets the OsCK1a.1 gene, utilizes CRISPR / Cas9 gene editing technology to construct a knockout vector for this gene, and successfully transforms it into rice, obtaining homozygous mutant plants with the OsCK1a.1 gene knockout. Experimental results show that, compared with the control, homozygous mutants of different mutation types exhibit significantly enhanced seed dormancy levels and substantially improved panicle budding resistance. Therefore, this invention, by knocking out the OsCK1a.1 gene, provides an effective method for creating new rice varieties resistant to panicle budding, which has significant application value for ensuring high and stable rice yields.

The application belongs to the technical field of jellyfish prevention and control, and discloses application of DMX-5804 in preparation of a medicine for inhibiting budding reproduction of Aurelia sp.1. By inhibiting budding reproduction of A.coerulea hydrocladium, the outbreak of Aurelia sp.1 can be prevented and controlled, and the adverse effects on marine ecological environment and human coastal economic activities can be reduced. Through experimental verification, 1 μM DMX-5804 can reduce the number of hydrocladium buds without affecting the living state of A.coerulea hydrocladium, which indicates that the compound DMX-5804 can inhibit the asexual reproduction of Aurelia sp.1 without toxicity at this concentration. The application provides a new theory and method for controlling the number of jellyfish, preventing the outbreak of Aurelia sp.1, and studying the biological conservative cell proliferation strategy.

The invention discloses an application of capsaicin in intestinal organ culture and a culture solution containing capsaicin, and belongs to the technical field of tissue culture. The intestinal organ culture solution comprises capsaicin with the concentration of 1.25-5 [mu] mol / L and a stem cell culture solution. When the culture solution is used in pig jejunum organ culture, generation and budding of jejunum crypt organs can be remarkably promoted, and cell growth and intestinal organ amplification are effectively promoted. According to the method for promoting amplification of the intestinal organs provided by the invention, many limitations of a traditional model can be overcome, and the development of intestinal pathology is assisted.

The present invention relates to the field of viral vectors, and in particular to a fusion polypeptide and a use thereof. Disclosed are a fusion polypeptide and a use thereof. The fusion polypeptide can mediate the budding of a pseudotyped LVV or RVV in packaging cells. Also disclosed is a particle comprising the fusion polypeptide, wherein the non-specific transduction capability of the particle is reduced.

The present application relates to the tissue culture technical field of Yunnan rhizoma paridis, and particularly relates to a method for establishing a high-efficiency tissue culture seedling system of Yunnan rhizoma paridis, comprising the following steps: S1. inducing Yunnan rhizoma paridis secondary buds; S2. activating the budding of Yunnan rhizoma paridis secondary buds; S3. growing Yunnan rhizoma paridis seedlings; S4. transplanting Yunnan rhizoma paridis seedlings; S5. repeating steps S3-S4 to grow and transplant seedlings for multiple times to obtain perennial seedlings of Yunnan rhizoma paridis. The present application overcomes the problem of "false seeds" of Yunnan rhizoma paridis, prevents the waste of a large number of seedlings, and first establishes a high-efficiency tissue culture system of Yunnan rhizoma paridis. The perennial tissue culture seedlings obtained through secondary bud induction, transplanting and re-induction have biological significance, a large number of Yunnan rhizoma paridis species resources are obtained, and the dilemma of the scarcity of Yunnan rhizoma paridis resources is effectively solved.

A method for high-efficiency regeneration of a potato protoplast is provided, including the following steps: S1, conducting test-tube seedling cultivation; S2, conducting protoplast preparation; S3, conducting callus proliferation; S4, conducting callus synchronization; and S5, conducting callus induction regeneration. Protoplasts derived from treated potato leaves are induced to form a callus. Through callus synchronization and callus induction regeneration, high-efficiency and synchronized budding is achieved, significantly shortening the regeneration timeline of protoplasts and markedly enhancing the bud regeneration rate. This method provides abundant experimental materials for potato genetic improvement and cultivar development, and ensures consistency and stability of regenerated plantlets. This method also offers technical support for downstream applications, including: genetic theory research, high-throughput virus-free plantlet regeneration, and gene-editing breeding in potatoes.

This invention discloses an InDel marker, Pmct1, for cold tolerance during rice budding, along with its identification method and applications, belonging to the field of molecular marker technology. This invention develops an InDel molecular marker by combining the insertion / deletion sequences of the Dongxiang wild rice introgression line D2 (background of the cold-tolerant rice 9311) and the cold-sensitive rice 9311 at the Chr1:32106831..32106871 locus in the O. sativa reference genome. Primers are then developed based on this molecular marker for identifying cold tolerance during rice budding. The detection cycle of this invention is only 2-3 hours, does not rely on subjective experience in traditional detection methods, and can obtain accurate and objective detection and identification results in a short time. It is low-cost, high-throughput, and highly specific, making it suitable for rice breeding and production practices.

The invention discloses application of PSK to improvement of asexual propagation efficiency of lily scales, belongs to the technical field of plant asexual propagation, and particularly aims to solve the problems that existing lily scale cutting propagation is low in efficiency, slow in budding and rooting and the like. The PSK with the effective concentration (preferably 10 nM <-1 > mu M) is prepared into a treatment solution; during cuttage, the water is applied as first-time irrigation liquid, and the physiological response of the scales can be systematically regulated and controlled. Experiments show that the application can significantly improve the budding rate of longya lilium scales, promote the expansion of new bulblets, and synchronously improve the rooting rate. The invention provides a novel efficient asexual propagation method for lily, which is simple and convenient to operate, remarkable in effect and safe, and is suitable for large-scale production of lily seedlings.