103results about How to "Improve breeding efficiency" patented technology

The invention relates to the field of genes, in particular to molecular markers for judging flowering time of brassica napus L. The molecular markers are Indel-282, Indel-326 and Indel-353 respectively and are located on an A02 chromosome of the brassica napus L.. The molecular marker has high specificity and stability, and is significantly related to the flowering time. The markers show high specificity and stability in brassica napus plants under different genetic backgrounds. By detecting the molecular markers through PCR amplification and electrophoresis, the flowering time of different plants can be quickly and accurately identified in the early stage of breeding. Therefore, the flowering time of the plant can be determined in the early stage, so that the breeding efficiency is improved. The molecular markers can be used for molecular marker-assisted selective breeding, and plants with early flowering or late flowering characteristics can be quickly screened by detecting the markers, so that the breeding process of excellent varieties is accelerated, and the functions and regulation mechanisms of related genes are further disclosed.

The invention relates to the field of molecular biology and genetic breeding, and provides application of an SNP (Single Nucleotide Polymorphism) marker related to a body height character. The SNP marker is located at the 136651710 bp position of the No.3 chromosome of sheep, and is Cgt; and T base mutation (rs403130818) is carried out. Experiments show that the SNP marker developed by the invention has significant influence on the sheep body height character (Plt; 0.05), and the allele T has more excellent body height character, so that the SNP molecular marker in the MCRS1 gene can be applied to molecular marker breeding of the sheep body height character. The molecular marker provided by the invention is not limited by the age, sex and the like of the sheep, can be used for breeding excellent body height sheep varieties, and even can be accurately screened when the sheep are newly born, so that the breeding process of the excellent body height sheep varieties is greatly accelerated.

The invention relates to the technical field of molecular breeding, and discloses a molecular marker related to the LDL-C level of porcine serum, a detection reagent and a breeding method. The invention also discloses a primer group for identifying the molecular marker, and the primer group is used for amplifying the following SNP sites: SNP01, which are obtained on the basis of a pig reference genome Sscrofa11.1 and are positioned on 112bp, 008bp and 261bp of a third chromosome, and the genotype is A / G; sNP02 is located at 112bp, 008bp and 354 bp of a third chromosome, and the genotype of SNP02 is A / G; sNP03 is located at 112bp, 008bp and 583bp of the third chromosome, and the genotype of SNP03 is A / G. The primer group for identifying the molecular marker can quickly and effectively identify the LDL-C level of pig serum, and is used for assisting in breeding pig strains with low cholesterol or specific lipid metabolism characteristics.

The utility model discloses a soybean hybrid breeding auxiliary mirror image device with amplification function relates to agricultural hybrid breeding technical field, including mirror frame, the inner wall fixed mounting of mirror frame has the lamp ring, the inner wall fixed mounting of lamp ring has the magnifying glass, and the bottom of mirror frame is installed with operation clamp through connecting component, through the mutual cooperation between connecting component, operation clamp, mirror frame and magnifying glass, and the breeding personnel can cover operation clamp on the finger, then will the magnifying glass aim at the flower organ of soybean, and then will the flower organ enlarged presentation, improve the precision of detasseling and pollination, and connecting component can adjust the rotation angle of magnifying glass simultaneously, make magnifying glass aim at the soybean of different height and area, and the suitability is strong.

This invention discloses a selection method for meat geese that comprehensively considers feed conversion efficiency and subcutaneous fat properties, relating to the field of poultry genetics and breeding. The method includes the following steps: S1 determining feed conversion efficiency of meat geese; S2 determining subcutaneous fat properties of meat geese; S3 calculating the family mean values ​​for each trait; S4 analyzing the correlation and heritability of each trait; S5 constructing a comprehensive selection index for feed conversion efficiency and subcutaneous fat properties of meat geese; and S6 selecting breeding geese with high comprehensive selection indices. This invention utilizes individual measurements and single-cage determination of feed conversion efficiency in meat geese, establishes a three-point method for accurately measuring subcutaneous fat thickness, and constructs a comprehensive selection index for residual feed intake, subcutaneous fat percentage, and subcutaneous fat thickness. It provides a selection method that comprehensively considers feed conversion efficiency and subcutaneous fat properties in geese, simultaneously improving feed efficiency and reducing subcutaneous fat properties, achieving simultaneous selection and synergistic improvement of multiple traits, and significantly enhancing breeding efficiency.

The invention discloses a DMP-based non-heading Chinese cabbage haploid creation method and application. According to the invention, gene editing is carried out on brassica rapa BrDMP, so that protein is lost or activity of brassica rapa BrDMP is reduced, and an obtained mutant plant is a non-heading Chinese cabbage haploid induction line; and carrying out selfing on the non-heading Chinese cabbage haploid induction line or the offspring of the non-heading Chinese cabbage haploid induction line, wherein the obtained selfing offspring is the non-heading Chinese cabbage haploid. The obtained haploid induction line of the non-heading Chinese cabbage can induce generation of haploid of the non-heading Chinese cabbage, and an effective way is provided for haploid breeding and double haploid creation of the non-heading Chinese cabbage.

The application discloses a sugarcane regeneration-promoting small peptide ScREF1 and application thereof, and belongs to the technical field of plant biotechnology and sugarcane tissue culture. The amino acid sequence of the ScREF1 is shown as SEQ ID NO. 1. The application provides a high-activity sugarcane-specific regeneration-promoting small peptide ScREF1, and provides a matching small peptide mother liquor preparation and callus induction / differentiation medium adding process, and the optimal application concentration is determined in stages. The ScREF1 provided by the application can overcome the defects of existing sugarcane tissue culture, such as serious callus browning, weak embryogenic callus proliferation, low regeneration efficiency and strong genotype dependence. The application can be widely applied to factory-like rapid propagation of sugarcane elite varieties, cultivation of virus-free seedlings, genetic transformation of transgenic materials, creation of CRISPR gene editing materials, and significantly improves the efficiency of sugarcane molecular breeding.

This invention relates to the field of plant genetic engineering technology, specifically providing... GmMYB4 Application of genes in regulating salt tolerance in soybeans. This invention utilizes cloning soybeans... GmMYB4 Genes were constructed, and overexpression and CRISPR / Cas9 gene knockout vectors were created. Soybeans were then transformed to obtain overexpression. GmMYB4 Strains and knockout mutants. Salt tolerance phenotype identification results showed that overexpression... GmMYB4 Significantly improves soybean tolerance to salt stress, while knockout GmMYB4 This reduces the salt tolerance of soybeans. Further DAP-seq and dual-luciferase experiments confirmed that the GmMYB4 protein can bind to and activate downstream peroxidase genes. GmPRX47 The expression of . This invention discloses for the first time GmMYB4 The function and mechanism of positive regulation of soybean salt tolerance provide important gene resources for breeding new salt-tolerant soybean varieties and have significant application value.

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to soybean mosaic virus resistance, an amplification primer and application of the SNP molecular marker in soybean breeding. The SNP molecular marker is located at the 910489bp site of a No.7 chromosome of a soybean genome, and the polymorphism of the SNP molecular marker is C / A. The invention provides application of the SNP molecular marker related to the soybean mosaic virus resistance in identification or auxiliary identification of the soybean mosaic virus resistance. By providing the accurate, simple, convenient and low-cost SNP molecular marker and the detection method thereof, early-stage, rapid and efficient molecular-assisted selection of soybean mosaic virus resistance characters is realized, so that the breeding process of a new variety of disease-resistant soybeans can be remarkably accelerated, and the SNP molecular marker has important breeding application value.

This invention provides a gene related to loquat flowering. SVP1 This invention relates to the field of molecular biology, specifically to a tightly linked SNP marker and its applications. The nucleotide sequence of this SNP marker is shown in SEQ ID NO.1. A genotype of C at the locus corresponds to a spring-flowering phenotype in loquat, while a genotype of G corresponds to an autumn-flowering phenotype. The invention also provides specific primers (SEQ ID NO.2 and SEQ ID NO.3) for detecting this SNP marker, a kit containing these primers, and a screening method for detecting loquat flowering time using this marker. By extracting loquat DNA and performing PCR amplification and sequencing analysis, the flowering period of loquat can be quickly and accurately determined. The SNP marker of this invention is highly linked to loquat flowering time, providing an effective tool for marker-assisted selection breeding of loquat at different flowering periods. This significantly improves breeding efficiency, shortens the breeding cycle, and is of great significance for extending the loquat fruit supply period and optimizing the industrial structure.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker for detecting the color of watermelon pulp and application of the SNP molecular marker. The SNP molecular marker corresponds to a Chr06: 24308944 site on a No.6 chromosome of a watermelon Citrullus lanatus 97103 v2.5 genome, the polymorphism of the SNP molecular marker is G / A, and the SNP molecular marker is closely linked with the thick yellow / light yellow color character of watermelon pulp. When the basic group of the SNP site is G, the watermelon shows a thick yellow pulp character; and when the basic group is A, the watermelon shows a light yellow pulp character. The invention also provides a KASP primer group for detecting the SNP molecular marker, and a detection method based on the primer group, and KASP-PCR amplification and genetic typing are performed on a sample to be detected, so that the color character of watermelon pulp is judged. The invention also provides a kit containing the primer group and application of the kit in watermelon molecular marker-assisted breeding. The method has the advantages of being rapid in detection, high in accuracy, free of environmental influence, suitable for high-throughput application and the like, and the breeding efficiency of the yellow pulp watermelon variety is improved.

This invention relates to the field of biotechnology and discloses a molecular marker, method, and application for identifying green-core soybeans. The molecular markers for identifying green-core soybeans are D1-dCAPS and D2-Indel. The method comprises: 1. Amplifying the D1-dCAPS molecular marker using a semi-nested PCR method with the DNA of the soybean material as a template; 2. Amplifying the D2-Indel molecular marker using a triplet PCR method with the DNA of the soybean material as a template; 3. Simultaneously satisfying the identification in step 1 as D1-dCAPS. Δ Soybean materials identified as having the D2-In genotype in step two are considered green-core soybeans. The method of this invention can distinguish different molecular marker banding patterns using agarose gel electrophoresis, which offers advantages over non-denaturing acrylamide gel electrophoresis in terms of safety, speed, and ease of operation. The molecular marker and identification method described in this invention can be used for the identification and screening of green-core soybean phenotypes, and can rapidly, accurately, and effectively breed green-core soybean varieties, accelerating the crop breeding process and greatly improving breeding efficiency and success rate.

The invention discloses a corn ZmMORF3 gene, an excellent haplotype of the corn ZmMORF3 gene and application of the corn ZmMORF3 gene in grain development regulation and molecular breeding. A key gene ZmMORF3 which affects mitochondrial gene RNA editing is cloned by using a grain mutant m497 found in a corn field breeding line selection process, and the gene encodes MORF protein and regulates assembly and activity of a mitochondrial compound I, so that corn grain development is affected; the excellent allelic variation genotype of the ZmMORF3 gene in the aspect of the row grain number is further excavated, and the row grain number can be increased by utilizing the ZmMORF3 gene, so that the yield is increased. In addition, distribution and utilization potentials of different haplotypes in a selfing line are researched, and technical support and excellent germplasm resources are provided for utilization of the gene.

This invention discloses a SNP molecular marker associated with the growth traits of *Bellamya pear-shaped* and its application, belonging to the field of marker-assisted breeding technology. The SNP molecular marker is located at loci 131,293,660 on chromosome 4 of *Bellamya pear-shaped* (T>C variant), where the T allele (genotypes T / T and T / C) is significantly positively correlated with growth rate and body weight. This invention integrates genome-wide association analysis (GWAS) with an XGBoost machine learning model to screen key loci and designs specific primer pairs to achieve accurate genotyping. Population validation shows that individuals carrying the T allele have significantly higher growth rates and average body weights than individuals with the C / C genotype. This marker can be used for early prediction of growth performance in *Bellamya pear-shaped*, selection of high-yielding varieties, and construction of genetic maps, overcoming the bottlenecks of long breeding cycles and low efficiency in traditional breeding methods.

The application discloses a wheat arabinoxylan content related molecular marker and application thereof. The application belongs to the technical field of biology, and provides a wheat genome SNP molecular marker and a substance for detecting the molecular marker, which are applied to any one of the following: (1) identifying or assisting in identifying wheat water-soluble arabinoxylan content; (2) wheat breeding; (3) preparing a product for identifying or assisting in identifying wheat water-soluble arabinoxylan content; and (4) preparing a wheat breeding product. The substance for detecting polymorphism and genotype of the above-mentioned SNP can be combined with other substances (such as a substance for detecting single nucleotide polymorphism or genotype of other wheat water-soluble arabinoxylan content related molecular markers) to prepare a product for identifying a wheat variety with wheat water-soluble arabinoxylan content, to perform early and efficient auxiliary screening on WE-AX content, and to improve breeding efficiency.

The application provides a SNP molecular marker related to a multiple lamb trait of sheep, a detection primer set, a kit and application thereof, and belongs to the technical field of SNP molecular markers. The application provides application of the SNP molecular marker related to the multiple lamb trait of sheep in detection of the multiple lamb trait of sheep or sheep breeding. ABCA1 The application provides a method for detecting single nucleotide polymorphism of ABCA1 gene of sheep and application thereof, and provides basic data for molecular assisted marker selection breeding of sheep and accelerates improvement of Chinese sheep germplasm resources.

The present application relates to the technical field of plant breeding, and particularly relates to a molecular marker and application thereof in breeding soybean varieties with high oil content and high protein content. The molecular marker is constructed based on a SNP site; based on the genome version number Wm82.a2.v1, the SNP site is located at the 8th chromosome of soybean at the 9020004th position, and the polymorphism is T / A. The application comprises: (1) predicting or detecting the oil content or protein content of soybean; (2) identifying or breeding soybean varieties with high oil content or protein content; (3) molecular marker assisted breeding of soybean; (4) improvement of soybean varieties related to oil content or protein content; (5) improvement of soybean germplasm resources. The molecular marker provided by the present application has the characteristics of double trait synergistic correlation, strong specificity, efficient detection process and the like, and can be widely applied to the scenes of molecular marker assisted breeding of soybean, rapid evaluation of germplasm resources and the like, and provides key technical support for breeding of soybean varieties with double optimal traits.

This invention discloses a specific molecular marker for identifying eggplant Saet CMS cytoplasmic male sterility lines and its application. The molecular marker, Saet218b, was selected by comparing the mitochondrial genomes of the eggplant Saet CMS cytoplasmic male sterility line and its maintainer line. Through bioinformatics analysis, the candidate specific gene for Saet CMS male sterility, orf218b, was obtained, and its nucleotide sequence is shown in SEQ ID NO: 1. Based on this sequence information, the molecular marker Saet218b for identifying eggplant Saet CMS cytoplasmic male sterility lines was successfully designed. The molecular marker of this invention can be used to identify eggplant Saet CMS cytoplasmic male sterility lines efficiently and with a simple procedure. The results can be used for marker-assisted breeding of eggplant Saet-derived cytoplasmic male sterility lines, improving breeding efficiency. It also provides technical support and theoretical basis for the identification of eggplant specific cytoplasmic germplasm.

This invention discloses a method for rapidly detecting conventional and hybrid varieties of industrial chili peppers and its application, relating to the field of molecular breeding technology. The method includes: extracting DNA from fresh leaves of the industrial chili pepper to be tested and performing whole-genome resequencing at a sequencing depth of 8×–12×; aligning the sequencing data to the chili pepper reference genome (Capsicum chinense cv. 'PBC932') to screen for high-quality heterozygous SNP loci; using a 1 Mb sliding window to statistically analyze the distribution density of heterozygous SNPs across the entire genome; and determining the variety type based on density thresholds: an average density <300 SNPs / Mb indicates a conventional variety, and >2000 SNPs / Mb indicates a hybrid variety. This invention eliminates the need for phenotypic observation in subsequent generations, requiring only leaves from current-generation plants to rapidly and accurately distinguish conventional and hybrid varieties at the molecular level. It features a short detection cycle, objective and reliable results, and is not limited by the number of molecular markers, providing strong technical support for efficient identification and early screening of industrial chili pepper germplasm resources.

The present application relates to the fields of molecular biology and plant breeding technology, and particularly relates to a primer pair for distinguishing between one-flowering and continuous-flowering Cymbidium varieties, application and kit thereof. The primer pair comprises FRI-M-F and FRI-M-R. The molecular marker FRI-M-F / FRI-M-R provided by the present application can accurately and efficiently distinguish between one-flowering Cymbidium varieties and continuous-flowering Cymbidium varieties, and can be used for molecular marker assisted screening and identification of the continuous-flowering trait of Cymbidium, thereby significantly improving the breeding efficiency.

The invention relates to the technical field of plant genetic engineering and molecular biology, and provides a BcRPGE1 gene for regulating petiole color differentiation of non-heading Chinese cabbage, and the nucleotide sequence of the BcRPGE1 gene is as shown in SEQ ID NO.1. The invention also provides an application of the gene in adjusting the petiole color differentiation of the non-heading Chinese cabbage and a specific method for adjusting the petiole color of the non-heading Chinese cabbage. In addition, the invention further provides application of the method in directional improvement of the petiole color of the non-heading Chinese cabbage and cultivation of non-heading Chinese cabbage varieties with different petiole colors. The BcRPGE1 gene has the advantages that the BcRPGE1 is determined as a key gene for regulating and controlling the petiole color differentiation of the non-heading Chinese cabbage for the first time, the directional regulation and control of the petiole color can be realized by regulating and controlling the gene, and a new gene resource and a technical support are provided for molecular regulation and control and breeding application of the petiole color of the non-heading Chinese cabbage.

This invention provides a method for the germination and regeneration of mature rubber tree embryos into seedlings outside of test tubes, belonging to the field of seedling cultivation technology. The method uses somatic embryos derived from anther tissue culture as raw materials, and involves three stages of cultivation in a growth medium: dark culture, low-light culture, and strong-light culture. Then, the mature embryos that meet the requirements undergo closed-bottle / open-bottle acclimatization. After this acclimatization, they can be rooted and grown into seedlings in river sand outside the test tube. This method allows mature embryos to germinate and regenerate directly into seedlings outside the test tube, eliminating the need for a sterile culture room and specialized rooting medium, thus requiring less specialized knowledge from the operator. Furthermore, by combining the embryo germination and seedling regeneration with the in-test-tube and sand-bed acclimatization in one step, the cultivation process is simplified, the cultivation cycle is shortened by two months, and cultivation efficiency is improved while production costs are reduced.

The invention relates to a grouper crossbreeding method. The method comprises the four steps of parent breeding, temperature-sensitive domestication, artificial spawning and insemination, incubation and fry breeding, specifically, cold water-based clear water stone spots and hot water-based brown spot stone spots are selected as parents, the clear water stone spots are used as female parents, the brown spot stone spots are used as male parents, and the middle water temperature is adapted through gradient temperature-sensitive domestication; stress-resistant feed containing hilsa herring egg source peptide and haematococcus pluvialis astaxanthin ester and a temperature-sensitive blender are matched, a reproductive care agent is supplemented to strengthen sperm and egg performance, and normative spawning induction, insemination and graded cultivation processes are adopted. According to the method, the problems of poor water temperature adaptation and low hatching rate of grouper hybrid sperms and eggs in different temperature areas are solved, the wide-temperature property of filial generations is improved, the method adapts to the water temperature of 18-28 DEG C, the hatching rate, the fry survival rate, the adult fish survival rate and the average weight of single fish are remarkably improved, and technical support is provided for industrial application.

This invention belongs to the field of wheat breeding, specifically relating to a wheat Fusarium head blight resistance locus and molecular breeding method. This invention identified five stable Fusarium head blight resistance-associated loci, Qfhb.jaas-2D, Qfhb.jaas-3A, Qfhb.jaas-3B.1, Qfhb.jaas-5A.2, and Qfhb.jaas-6D, on chromosomes 2A, 2D, 3A, 5A, and 6D. For the identified resistance loci, this invention designed KASP molecular markers. These markers have high specificity and sensitivity, enabling accurate and rapid detection of resistance genotypes in wheat materials, providing a powerful tool for marker-assisted selection (MAS). This invention verified the effectiveness of the screened resistance loci. The results showed that the above five loci were significantly associated with Fusarium head blight resistance, providing a reliable basis for the breeding of resistant varieties.

The application of broccoli genes BoSULTR1;1 and BoSULTR1;2 in improving plant stress resistance belongs to the technical field of plant genetic engineering. In order to solve the technical problem of how to improve the stress resistance of broccoli plants, the application provides the broccoli genes BoSULTR1;1 and BoSULTR1;2, and the application uses an expression vector or a recombinant bacterium overexpressing BoSULTR1;1 and BoSULTR1;2 genes to prepare a transgenic broccoli plant. The stress resistance related detection results prove that the BoSULTR1;1 and BoSULTR1;2 genes have the ability to improve the resistance of the transgenic broccoli plant to cotton bollworm, pathogenic bacteria and drought. The transgenic material obtained by the application has important theoretical significance and practical value for accelerating the breeding process of stress-resistant plants and improving the breeding efficiency.

The invention relates to the technical field of eucommia ulmoides breeding. The invention provides a breeding method and an identification method of triploid eucommia ulmoides, which comprises the following steps: taking eucommia ulmoides branches, cutting, treating with colchicine solution, and culturing to obtain adventitious buds; cultivating the adventitious buds to obtain seedlings, performing ploidy identification on the seedlings, and screening out tetraploid plants; taking the tetraploid plant as a female parent, hybridizing with the diploid male parent, and harvesting triploid eucommia ulmoides seeds. The invention provides the method for efficiently and stably obtaining the homozygous tetraploid eucommia ulmoides clone, the problems of common chimeras and unstable materials in chemical induction are solved, and reliable parents are provided for cross breeding. A set of complete technical system for directionally creating the triploid eucommia ulmoides by taking tetraploid as a female parent and diploid as a male parent (reverse crossing) is established, and the technical blank from creation of a polyploid parent to breeding of a new triploid variety is filled.

The invention discloses a multi-technology integrated rapid ginseng breeding method and application, and the method comprises the following steps: S1, explant screening and gradient sterilization: selecting rhizome buds of three-year-old healthy ginseng as explants, firstly soaking the explants in 75% ethanol for 30 seconds, then disinfecting the explants with a 0.1% mercury bichloride solution for 8-10 minutes, then washing the explants with sterile water for 5-6 times, and carrying out gradient sterilization on the explants; after moisture is absorbed by sterile filter paper, inoculating to a callus induction culture medium, and culturing for 25-30 days at the temperature of 22 + / -1 DEG C under the dark condition, so as to obtain healthy ginseng calluses; the callus obtained in the step 1 is subjected to ray irradiation mutagenesis, the irradiation dose is controlled to be 20-30 Gy, and the irradiation rate is 1 Gy / min. According to the method, the defect that a traditional ginseng breeding period is too long can be overcome, the breeding period is shortened, the callus induction rate, the cluster bud differentiation rate and the acclimatization and transplantation survival rate are greatly improved, precise screening of ginseng strains is achieved, and the industrial application cost is greatly reduced.

The invention relates to the technical field of animal breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker and application thereof in identifying pheasant laying number traits. On the basis of a genome version ASM 414374v1, the SNP molecular marker comprises any one or more of the following components: NW022205446.1: 5714958, NW022205446.1: 5715290, NW022205446.1: 5726623, and NW022205446.1: 5743232, and the SNP molecular marker comprises any one or more of the following components: NW022205446.1: 5743232. Four SNP molecular markers related to the pheasant laying number character are obtained through research and screening, and prediction and identification of the pheasant laying number can be achieved by detecting the polymorphism of the SNP molecular markers. The SNP molecular marker provided by the invention can be used for improving the laying number character of pheasants, and has important application value.

The present application relates to a kind of KASP molecular markers related to common pakchoi leaf purple and its application, by QTL-seq technology and genetic map location, the main effect QTL site related to leaf purple located on the 3rd chromosome of common pakchoi is found, and further developed the molecular marker closely linked to site, by the seed or seedling leaf extraction DNA of color unknown common pakchoi material, combined with the KASP primer of the present application carries out KASP molecular marker genotyping test, the color of common pakchoi can be predicted by the genotyping result of marker, without using artificial identification method, can obviously improve breeding efficiency, save breeding cost.