344results about How to "Improve breeding efficiency" patented technology

The invention provides a functional molecular marker for rice anti-blast gene Pi9 and application thereof, belonging to the field of crop molecular genetic breeding science. The invention finds out that the rice anti-blast gene Pi9 has a 10bp insertion/deletion site positioned between the gene promoter -516 and -517, wherein the nucleotide sequence is disclosed as SEQ ID NO.1; and on such basis, the invention develops a method of the functional molecular marker for gene Pi9. By detecting the functional molecular marker, the invention can accurately detect whether genomes of different rice species contain the Pi9 functional gene and detect the homozygotic state, and can be used for screening rice hybrid transformed descendant plants to enhance the breeding efficiency of the rice anti-blast material, thereby effectively controlling the size of the breeding population, obviously saving the breeding and screening cost and obtaining the anti-blast rice species containing the Pi9 functional gene.

The invention belongs to the technical field of cotton molecular breeding, and discloses SNP molecular markers associated with fiber strength of upland cotton as well as detection of the SNP molecular marker and application of the SNP molecular marker. The SNP molecular marker takes a cotton stable RIL group as a material, and is obtained through a genome re-sequencing method. The SNP markers are utilized to perform molecular marker assisted breeding, so that the breeding period can be greatly shortened, cotton fiber strength is enhanced, and the breeding efficiency is improved.

The invention belongs to the technical field of molecular breeding of cotton and discloses an SNP molecular marker associated with fiber strength of upland cotton as well as detection and application thereof. The SNP molecular marker is obtained by taking stable RIL group of cotton as the material through a genome resequencing method. When the SNP markers are used for carrying out molecular marker-assisted breeding selection, the breeding period can be greatly shortened; the breeding efficiency of the cotton can be improved; the cotton fiber strength can be improved.

A plant growing device (1) is provided with a ventilation unit (6) comprising an intake unit (61) for taking air into a growing chamber (R) and an exhaust unit (62) for exhausting air from the growing chamber (R). The intake unit (61) has an intake port (63), an intake damper (64) for opening and closing the intake port (63), a blower (65) for sending air in the direction of the growing chamber (R), a passage (66) for guiding air to the upstream side of the blower (65), and a circulation damper (67) for opening and closing the passage (66). The exhaust unit (62) has an exhaust port (68) and an exhaust damper (69) for opening and closing the exhaust port (68). The ventilation unit (6) operates under either an intake and exhaust mode for taking air into and exhausting air from the growing chamber (R) or a circulation mode in which air is caused to circulate in the growing chamber (R). By switching between these modes, CO2 is taken into the growing chamber (R) and gas density, temperature, and humidity within the growing chamber (R) can be made uniform, and as a result, the growing efficiency of a plant (P) can be improved.

The invention discloses a method for doubling management of corn haploid. The method for doubling management of corn haploid comprises the following steps: (1) cutting the tip of coleoptile of the corn haploid bud, but not damaging the main leaf and the growing point to obtain the corn haploid bud with coleoptile tip cut; and (2) soaking the corn haploid bud with coleoptile tip cut with a mixed solution of colchicine, dimethyl sulfoxide and water to obtain a chromosome doubled corn bud. The method for doubling management of the corn haploid can remarkably improve the doubling efficiency of the corn haploid, and the tassel pollination rate is over 30 percent which is much higher than the contrast tassel pollination rate (lower than 5 percent). The method solves the vital technical problem of low doubling efficiency in the haploid breeding process, so that the haploid breeding efficiency is greatly improved, and large-scale application of the haploid breeding technology becomes possible.

The invention discloses a rice dwarf-related protein and a coding gene and application thereof. The protein is protein (a) or (b): protein (a) is formed by an amino acid sequence shown as a sequence 3 of a sequence list; and protein (b) is formed by substituting and/or lacking and/or adding one or more amino acids in the amino acid sequence shown as the sequence 3 of the sequence list, related to rice dwarf and derived from the protein (a). The invention also provides the gene for coding the protein and a recombinant expression vector containing the gene, a transgenic cell system or recombinant bacteria. The gene is utilized to breed rice with reduced strain height. The rice dwarf-related protein and the coding gene have extremely important values of shortening the breeding period, improving the breeding efficiency and enriching rice dwarf sources.

The invention relates to a method for cultivating cross rice. Wherein, it comprises that (1), selecting the light-temperature sensitive sterile system with low-temperature sterile or short-light low-temperature sterile; (2), selecting the sterile masculinity as gene system; (3), crossing sterile system S with sterile masculinity system S', to obtain stable sterile masculinity S/S' F1; (4), screening and recovering system R; (5), using S/S' F1 as mother type to be crossed with recover system R, to find the needed cross combination, to produce cross rice. The invention can improve the match property of sterile system and reduce produce cost.

The invention relates to a breed conservation, screening and breed production method in tilapia complete set lines, comprising the following steps of: construction of complete set line base populations rich in hereditary variation, establishment of operation procedures of family production and management in the complete set lines, marking of individuals in the complete set lines, genetic evaluation analysis of important production traits and establishment of individual mating selection programs in the complete set lines. The invention also provides a hybrid breed production method between twolines of complete sets, which is established by utilizing the method. The method disclosed by the invention has the characteristics of high pertinence, high selection breeding efficiency, capability of effectively preventing parent inbreeding depression of the complete set lines and the like; the harvest weight of the hybrid fine breed of tilapia is averagely increased by more than 30% than that of control groups; and the culture survival rate is also obviously higher than that of the control groups. According to the invention, a new technical approach is developed for cultivation of fish fine breeds; particularly a new selection breeding method is applied in the breed conservation, the screening and the breed production of the complete set lines in fish hybrid breeding; and the method isalso suitable for being popularized and applied in all cultured fishes and has broad popularization and application prospects.

The invention discloses a molecular label linked with a major gene of a high-strength cotton fiber, which is obtained by the following method: taking the Xinluzao No.24 as a male parent, taking Lunianyan No.28 and Ji cotton 516 as female parents; respectively constructing two segregation populations of F2 and F2:3; carrying out polymorphism screening between parents (Lunianyan 28 and Xinluzao 24)by utilizing an SSR (simple sequence repeat) primer; constructing a genetic linkage map by utilizing F2; carrying out two generation quantitative trait locus (QTL); selecting an SSR label linked withthe high-strength cotton fiber to analyze the DNA polymorphism of the parent Xinluzao 24 and the Ji cotton 516; and constructing the genetic linkage map and positioning QTL; the fiber strength major QTL (qFS-62) can be stably detected in two populations and generations, and is positioned between the label interval of DPL0757-DPL0920 of chromosome c7; a synergtic gene is from the parent Xinluzao No.24; and the linking label of qFS-6-2 is DPL0757130, DPL0852170 and DPL0920130. By utilizing the label provided by the invention, molecule label assisted selection is carried out, thus improving the selection efficiency of the high-strength cotton fibre.

An efficient aggregate method for breeding seedless grap is disclosed, which features that the conventional hybridizing technique, the embryo retriever technique and molecular marker breeding technique are integrated. Its advantages are short breeding period shortened from 7-9 years to 3-4 months, high resistance to disease, high seedless percentage, and high quality of grape.

The invention provides a breeding method for a multi-resistance flue-cured tobacco hybrid. The breeding method for the multi-resistance flue-cured tobacco hybrid comprises the steps of firstly using MSG28 as a female parent, using high-quality, widely applicable and blackleg-resistant varieties which are sensitive to TMV and weather fleck and are used for production as recurrent parents, performing eight generations of backcrossing to breed male sterile lines, using fertile materials which are resistant to blackleg and highly resistant to TMV and weather fleck as original resources, performing six generations of selfing purification to breed selfing pure lines with common or better curing characteristics, then using the bred male sterile lines as female parents and the selfing pure lines as male parents to breed hybrid combinations, respectively evaluating resistance to different diseases and economic performance by using land parcels or areas seriously attacked by different diseases and normal land parcels in producing areas, and finally performing 2-3 years of multi-point tests for quality and adaptability evaluation to the hybrid combinations which are simultaneously resistant to different diseases and have good economic performance. By adopting the method, the aggregation of multiple characters such as high quality, simultaneous resistance to foliage diseases and root and stalk diseases and good adaptability can be realized, the breeding efficiency is improved.

The invention relates to a single strand conformation polymorphism (SSCP) marker closely linked with a major wheat scab resistance quantitative trait locus (QTL). The SSCP marker is characterized in that: scab resistance candidate genes of a scab resistance QTL in a 3BS area of a wheat variety or strain resisting scab is PCR amplified by using a primer; after denaturalization, a PCR-amplified product has different single strand conformation; and the SSCP marker closely linked with the major wheat scab resistance QTL is established for detecting a genotype of the wheat variety or strain during breeding. The SSCP marker has the advantages of: overcoming the disadvantages that the wheat scab resistance screening can only be authenticated in a flowering period and is easily influenced by the environment in conventional breeding, predicting and screening wheat plants with the scab resistance by detecting a molecular marker at a seedling stage, eliminating disease plants, reducing waste of labor and materials and improving the breeding efficiency. Compared with an ABI DNA sequence analysis meter-based marker, the SSCP marker closely linked with the major wheat scab resistance QTL has the advantages of simple and convenient operation, low cost and same sensitivity.

The invention relates to a breeding and cultivating method for a plant, in particular relates to a breeding and cultivating method for loose purple cauliflowers, and belongs to the technical field of plant cultivation. The breeding and cultivating method comprises the steps of hybridizing a loose purple cauliflower breeding material with a semi-compact purple cauliflower breeding material to obtain seeds F1, and selfing single strains with high comprehensive agronomic characters after the seeds F1 are sowed to obtain seeds F2; performing microspore culture on single loose purple cauliflowers through flower buds suitable for the microspore culture from the mononuclear edging stage to the double-core early stage to grow a large number of normal regenerated plants, selfing excellent DH plants of the loose purple cauliflowers to obtain a loose purple cauliflower excellent DH strain 03-12 serving as a recurrent parent which is hybridized with a cauliflower cytoplasmic male sterility source material CMS05-6, performing five generations of backcross transformation and selection to form a cytoplasmic male sterile line CMS08-2, and hybridizing the DH strain serving as a parent with the excellent cytoplasmic male sterile line CMS08-2.

The present invention discloses a method for rapid in-vitro propagation of a Crassulaceae plant. The method comprises the steps of carrying out aseptic germination of seeds, inducing adventitious bud, and carrying out in-vitro rooting of the adventitious buds. The method allows in-vitro germinated seedlings to normally grow and to be induced within a short period of time to continuously differentiate from original single-seed single seedlings into single-seed multiple seedlings, so the propagation cycle is greatly shortened, and the growth cycle of the differentiated seedlings is greatly shortened. The method provides a stable technical platform for improvement of the breeding efficiency of high-quality varieties of the Crassulaceae plant.

The invention discloses a culture method for triploid grape culture by embryo rescue and early identification of ploidy. The invention aims to cultivate triploid grape by embryo rescue and overcome the problem of seed abortion of hybrid embryo between diploid and tetraploid grape cultivars in conventional breeding. After a hybrid seed is obtained, chromosome count is performed by the combination of flow cytometry and wall degradation hypotonic method to identify the ploidy of the hybrid progeny. The invention increases the breeding efficiency of triploid grape, accelerates the breed selection of triploid grape variety and provides a new approach to the culture of seedless grape variety.

The invention relates to a method for breeding raphanus sativus L. CMS (genic male sterility) lines by using marker assisted selection, which belongs to the technical field of crop breeding and is used for efficient breeding of excellent male sterility lines in raphanus sativus L. hybrid advantage utilization. The invention adopts the technical scheme that: the method comprises the following steps of: screening inbred lines with excellent traits and stable fertility; selecting specific primers NAUmsF4 and NAUmsR4 to perform PCR amplification on the screened inbred lines, and determining that the inbred lines capable of amplifying 1069bp specific strips cannot be used as candidate maintenance lines; performing restored gene functional marker analysis on the inbred lines incapable of amplifying the specific strips, and screening the inbred lines capable of serving as the candidate maintenance lines; and performing cross and back cross of 4 to 5 generations on the original sterile lines and the identified candidate maintenance lines to obtain new sterile lines and corresponding maintenance lines with excellent traits. The method for quickly screening the candidate maintenance lines by using molecular makers is not affected by a growth period and an environment, can remarkably improve the breeding efficiency of the raphanus sativus L. CMS lines, and accelerates the breeding progress of excellent F1 hybrids.

The invention provides a herbicide-resistant rape directive breeding method based on acetolactate synthase (ALS) target esterase, which belongs to a plant trait breeding method. The method comprises the following steps of selecting materials needed by production of rape or breeding of the rape, processing seeds with ethylmethane sulfonate (EMS), mutagenizing the seeds to be isolated and propagated to a M2 generation, and directionally screening herbicide-resistant mutant characters in a large group under the selection pressure of the herbicide adopting the ALS as the target esterase. In order to increase the probability for acquiring the mutant characters, the directive screening can be continuously conducted in multiple years, or conducted in multiple places in the same year or conducted in multiple places in multiple years until the needed mutant material is screened out.

The invention relates to a cultivation method of economic seaweed, in particular to a full-artificial seed cultivation method for sargassum horneri. Concretely, the method comprises the following steps: selecting male and female sargassum horneri parent strains with good pyrenium growth to be cultivated for 10 to 20 days under the conditions that the illumination intensity is between 2500 and 10000 luxes and the temperature is between 10 and 16 DEG C for realizing the synchronous sexual development and sexual maturation of the sargassum horneri so that ova and sperms are discharged synchronously for impregnation; completing the collection and the seedling distribution of germ cells in 10 to 24 hours after the impregnation; carrying out static cultivation for 2 to 4 days after the seedling distribution; and maintaining the water flowing under the condition of the illumination intensity between 400 and 8000 luxes for completing the cultivation. The seed cultivation process of the invention is carried out in an artificial cultivation system, so the impregnation rate can be further and greatly improved, the spatfall efficiency and the full-artificial seed cultivation efficiency can be improved.

The invention discloses a method for rapidly obtaining a large number of wheat double-haploid homozygous populations. According to the method, based on a wheat and corn double-haploid breeding technology, through the key links of wheat and corn sowing at different stages, haploid induction, transplantation and vernalization, reduplication and transplantation and winter sowing treatment, after wheat young embryos differentiate into haploid seedlings, transplantation, vernalization and haploid plant reduplication are completed in the same year without over-summer treatment, wheat double-haploid seeds can be harvested in the same year, the requirement for sowing once more can be met after the harvest on the perspective of time, a large number of stable new strains of the wheat homozygous populations can be obtained within one year, the requirement for identifying the new strains at the next stage is met, the natural environment is utilized to the maximum degree, and the time of wheat double-haploid breeding is shortened to one year from two to three years. In this way, the wheat breeding speed is increased, the breeding efficiency is improved, the breeding cycle is shortened, and the breeding cost is lowered.

The invention relates to a floating seedling raising method for sweet potato true seeds, and belongs to the technical field of sweet potato true seed seedling raising.The method specifically comprises the steps that 1, a floating seedling raising pond is built and filled with water; 2, 200-pore foam floating discs are selected and filled with a seedling raising matrix with the water content of 60%; 3, the germinated sweet potato true seeds which are treated through 98% concentrated sulfuric acid and subjected to hot water and agentia soaking treatment are selected to be seeded and covered with soil, the soil surface is leveled, and the seeds are put into the seedling raising pond to be cultured; 4, water is supplemented for once every about 15 days during culturing; 5, seeding raising fertilizer is applied for the first time 3-5 days after seedlings are neat, the application amount is 15 g/disc, the concentration of nitrogen is kept to be 300 mg/L, and top dressing is performed for once every 7 days; 6, the seedlings reach the strong seeding standard after the seeds are seeded for 30-40 days and can be transplanted together with the soil.According to the floating seedling raising technique, quick and neat seedling emergence of the seeds can be promoted, the seedling emergence rate can reach 100%, the seedlings can be neat after emerging for 3-4 days, growth of the true seedlings can be kept consistent, the seedlings are strong, the root systems are developed, the root-top ratio is high, the plant height is appropriate, and time and labor are saved in the whole process.

The invention relates to molecular markers of paddy endosperm low amylose content gene Wx-mq, which belong to the field of molecular genetics. Two pairs of PRC molecular marker primers for specifically amplifying paddy endosperm low amylose content control gene Wx-mq and an allelic gene Wx-b thereof are used, wherein a forward general primer 1Wx-b-F of the Wx-b and the Wx-mq, a reverse primer 11Wx-b-R of the Wx-b and a reverse primer 11Wx-mq-R of the Wx-mq perform common amplification on the DNA of japonica rice varieties or materials, and can distinguish genotypes of the Wx-mq gene and the allelic gene thereof accurately. Developed peculiar PCR markers of the allelic gene are utilized to perform the paddy endosperm low amylose seed breeding and the improvement of rice quality.

The invention provides a cross breeding method for a Pelteobagrus fulvidraco variety namely Huangyou No. 1 with improved growth character. The cross breeding method comprises the following steps: 1, selection of rapidly-growing cross parents: 1.1, selection of a female parent, and 1.2, selection of a male parent; 2, strengthening breeding of parents; 3, artificial parturition hastening; 4, artificial cross fertilization; 5, artificial incubation; and 6, fry breeding. The cross breeding method provided by the invention breeds the first-filial generation of Huangyou No. 1 by cross breeding of female Pelteobagrus fulvidraco and pelteobagrus vachelli; and the first-filial generation of Huangyou No. 1 is tawny in body color, and has extremely significant growth vigor and strong stress resistance.

The invention discloses a magnaporthe oryzaea virulence gene AvrPik/kp/km/kh/7 and an application thereof. The new magnaporthe oryzae avirulence gene AvrPik/kp/km/kh/7 has DNA (Deoxyribose Nucleic Acid) sequences with promoters indicated by SEQ ID No. 1 and cDNA (Complementary Deoxyribose Nucleic Acid) sequences indicated by SEQ ID No. 2. The gene has three SNP (Single Nucleotide Polymorphism) haplotypes in the natural world. The invention also relates to a molecular target for designing novel pesticides according to the structure of the gene and the function thereof; the gene and a corresponding disease-resistant gene are covalently guided into oryza sativa L and other host plants to cultivate disease-resistant varieties; a specific molecular marker generated by the gene sequences is applied to the monitoring of magnaporthe oryzaea populations in a field; and the disease-resistant varieties are guided to be applied to the reasonable layout according to the monitored results.

The invention belongs to the field of rape breeding, in particular to a method for breeding a cabbage rape self-incompatible line. The invention aims at the problem of limiting the large area popularization of self-incompatible hybrids caused by the difficult massive propagation of the cabbage rape self-incompatible line, adopts the methods of auxiliary pollination of NaCl liquid plus honey and system selection to overcome the breeding barrier of the cabbage cole self-incompatible line, adopts the measures of early sowing, thin planting and high fertilizer and the like to culture a large-plant type cabbage rape self-incompatible line with a plurality of branches and adopts the selection methods of quality analysis and compatibility authentication and the like to obtain the protospecies of the cabbage rape self-incompatible line, thus further obtaining the protospecies of the cabbage rape self-incompatible line. The invention provides a simple and effective method for the massive propagation of the cabbage rape self-incompatible line and promotes the utilization of the advantages of the self-incompatible hybrids.

The invention discloses molecular markers closely linked to an RBSDV (rice black-streaked dwarf virus) major QTL (quantitative trait locus). The essence of the invention is that according to the linkage and segregation rules, the F2 population of the hybrid generation of Tetep and RBSDV susceptible varieties is taken as the mapping population and the SSR (simple sequence repeat) markers RM5626 and RM7097 on the No.3 chromosome, which are closely linked to the RBSDV resistant major QTL are obtained through analysis of linkage between the results of evaluation of disease resistance and the SSR marker data. Whether a plant has resistance to RBSDV can be judged by detecting the SSR marker banding pattern of the single rice plant of the hybrid combination generation of Tetep and derived varieties (lines) thereof and the RBSDV susceptible varieties. After the SSR markers are applied to assistant selection breeding of the RBSDV resistant varieties of Tetep and derived varieties (lines) thereof/RBSDV susceptible varieties, the defects of poor stability and repeatability of RBSDV resistance evaluation, time and labor waste, high technical threshold and the like in conventional breeding can be overcome and the results can conduce to simplifying the selection method and improving the breeding efficiency and then accelerating the breeding process of RBSDV resistant varieties.

The invention relates to a breeding method of multi-resistance rice germplasm, belonging to the technical field of biology. The breeding method is as follows: a parent P1 simultaneously carrying anti-xanthomonas oryzae gene Xa21 and Xa23 and a parent P2 simultaneously carrying anti-rice blast disease gene Pi-Kh and Pi-1 as parent raw materials, and Pi1, PiKh, Xa21 and Xa23 dispersed in different resistant sources are guided into the same rice germplasm by convergent cross and molecular marker-assisted selection technology, so that the new rice germplasm carrying multi-resistance-section is created, and the resistance of the parent of good hybrid rice can be improved rapidly by utilizing the multi-resistance germplasm. The germplasm can be directly used for preparing anti-disease hybrid rice combination, and also can be taken as the resistant source of the parent of a new circle of anti-disease hybrid rice. More importantly, a plurality of multi-disease genes can be guided into the same one breeding group by one-time hybridization of the multi-resistance germplasm, so that the breeding time is shortened greatly, and the breeding efficiency is improved.

The invention discloses a bisexual flower breeding method of manihot esculenta grant, and belongs to the technical field of agricultural cultivation. The breeding method comprises steps as follows: planting, top application, secondary top application, hormone spraying, nutritional regulation before flowering, pinching, pollen viability determination, crossbreeding, pollination, selfing isogeneity and final-period management. With the adoption of the breeding method, on the one hand, the problem of the small quantity of pollinating female flowers during crossbreeding of manihot esculenta grant is solved, more female flowers with different genotypes are provided, and the crossbreeding efficiency is improved; on the other hand, the bisexual flower of manihot esculenta grant is discovered for the first time, and an effective isogenic method adopting a manihot esculenta grant inbred line is provided; and further, the bisexual flower is formed through induction for the first time in the manihot esculenta grant breeding technology, the bisexual flower is used for crossbreeding, a novel crossbreeding method is created, and a new field is created for manihot esculenta grant hybrids.

The invention relates to a crop breeding method, in particular to a radish breeding method, belonging to the technical field of plant breeding and cultivation. The breeding method is characterized in that Kexing red-core and red-skin radishes are taken as female parents, wherein the Kexing red-core and red-skin radishes come from Sichuan province, have a moderate maturity period and upright leaf bunches, contain high-content anthocyanin, are high-yield, disease-resistant, red-skinned and have red flesh and long cylindrical flesh roots; Hengfeng transparent-core red radishes as male parents, wherein the Hengfeng transparent-core red radishes come from Chongqing, have a moderate maturity period and half upright leaf bunches, contain high-content anthocyanin, are heat-resistant and red-skinned and have red flesh, long cylindrical flesh roots and slightly larger lower parts; and the female parents are hybridized with the male parents to obtain intervarietal hybrids F1, and the intervarietal hybrids F1 are continuously self-bred to obtain a new high-quality, high-yield, high-anthocyanin-content and disease-resistant red-skin and red-core radish variety, i.e. Yanzhi No.1. The radish breeding method has the beneficial effects that the breeding efficiency is high, the obtained products are suitable for the extraction processing of anthocyanin and the problem existing in the raw material supply in the anthocyanin extraction processing industry is solved.

The invention discloses a method of a novel technology for artificially simulating a crop growth shade and humidity environment and particularly relates to a novel method for artificially simulating the crop growth shade and humidity environment. According to the method, on the basis of a steel frame greenhouse, power-driven sunshade nets are installed at the top and on the two sides of the greenhouse for controlling illumination in the greenhouse, a spraying system composed of a PPR pipe and a spray nozzle is erected inside the greenhouse for controlling humidity and temperature in the greenhouse, ventilation systems composed of gauzes and power-driven exhaust fans are installed in the middle portions of the two sides of the greenhouse and used for controlling the temperature and the carbon dioxide concentration in the greenhouse, and according to comparison of temperature, humidity and other meteorological data on agricultural environment monitors installed inside and outside the greenhouse, light controlling, humidifying, cooling and ventilating measures are taken, so that the shade and humidity environment which is remarkably different relative to local natural environment conditions is formed. The step that according to the comparison of the temperature, humidity and other meteorological data on the two agricultural environment monitors inside and outside the greenhouse, the corresponding light controlling, humidifying, cooling and ventilating procedures are adopted is the key. The shade and humidity environment is simulated through the method, and the method has the advantages of being wide in geographical adaptability and suitable for different types of crops needing shade-tolerance and humidity-resistance screening and improving shade-tolerance and humidity-resistance breeding efficiency.

The invention provides a hibernating conservation asexual reproduction method for jellyfish hydranth. The method is characterized in that when normal cultivation of young jellyfish is finished in early summer every year, an enough quantity of jellyfish intestinal-shape young fingerlings are purchased and subjected to conservation, illumination, feeding, trace-amount inflation, water changing, asexual reproduction and development under the condition of low temperature. The method improves the efficiency and benefit of cultivating jellyfish fingerlings by reducing or avoiding a sexual reproduction process of fingerling cultivation, has a simple structure, reduces production cost, and is suitable for extensive popularization.