548results about How to "Improve breeding efficiency" patented technology

The invention relates to the technical field of molecular markers, and discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the litter size character of pigs and application of the SNP molecular marker. The SNP molecular marker is located at a 34977540bp site of a chromosome 2 of an international pig reference genome Sscrofa11.1 version, and the nucleotide sequence of the site is G or A and is located in an exon region of an LUZP2 gene. The invention also discloses an application of the SNP molecular marker in breeding of the litter size character of the boar, and provides a method for breeding or auxiliary breeding of the boar with high / low litter size based on the marker. The invention also provides a specific primer for identifying the SNP site. By applying the technologies, accurate prediction and efficient screening of the litter size of the large white pigs can be achieved, the breeding efficiency of excellent breeding pigs is remarkably improved, and the population improvement process is accelerated. The invention also provides important technical support for sustainable development of pig raising industry, also provides theoretical basis for research in related fields, and is expected to promote further development of pig genetic improvement work.

The invention discloses a molecular marker for detecting a gibberellic disease resistant QTL (Quantitative Trait Loci) QFhb-yaas-4AL and application of the molecular marker. Genotype data are obtained by utilizing a Wheat16K wheat high-throughput gene chip, a QTL locus QFhb-yaas-4AL which is derived from a resistant material and is related to the resistance of the gibberellic disease is detected, and a KASP marker and a detection primer group thereof are developed and are used for efficiently screening the resistance of the gibberellic disease. The primer group disclosed by the invention is used for performing PCR amplification on wheat genome DNA, whether a gene with high gibberellic disease resistance of a resistant material is carried or not can be directly judged through KASP typing, the detection method is simple to operate, the detection result is very intuitive, the detection effect is obvious and effective, and the primer group can be widely applied to the field of genetic engineering. When the molecular marker is used for screening, the working efficiency of molecular marker-assisted selection of wheat breeding with high and low gibberellic disease resistance can be greatly improved.

The invention belongs to the technical field of gene breeding, and discloses a rapid matching breeding improved parent composition, an obtaining method and application. According to the method, a basic SNP marker is obtained; carrying out GWAS analysis on the basis of the collected phenotypes, carrying out dominant genotype identification on markers associated with the phenotypes, and constructing a dominant genotype library of the functional sites; calculating the contribution degree PVE of the functional sites to phenotypes, analyzing the contribution degree PVE, calculating the linkage region Block of each material or group, and constructing a material PVE score library and a Block library; and matching and combining every two materials based on dominant genotypes, PVE of functional sites and linkage regions Blocks of the materials / groups to obtain an optimal combination parent of each material. According to the invention, more favorable genotype polymerization states can be realized.

The invention discloses a cultivation method for promoting rapid growth and development of spring wheat, and belongs to the technical field of wheat breeding and cultivation. The cultivation method comprises the following steps: 1, seed pretreatment; 2, field planting and soil culture: transplanting wheat seeds with white buds into a seedling culture hole tray, transferring the seedling culture hole tray into a culture room with a long photoperiod, culturing until the wheat seeds are mature, and harvesting; lED light is adopted in the culture room for illumination; the LED light is blue light, white light or a mixture of red light and blue light according to a light quantum ratio of 3: 1. The method has the beneficial effects that the condition combination of regulating and controlling the light period, the light quality and the light intensity is provided. Through the combination of fixed and widely applicable illumination conditions, the method significantly shortens the growth cycle of the spring wheat. The method has the beneficial effects that the time from seed germination to mature harvesting of the spring wheat is generally 50-60 days, so that 6-7 generations of spring wheat can be harvested in one year. The wheat growth cycle is shortened, the breeding efficiency is remarkably improved, and a solid technical foundation is laid for improvement and cultivation of chassis varieties.

The invention discloses an SNP molecular marker related to the meat percentage character of procambarus clarkia and application of the SNP molecular marker, genetic typing is performed on the procambarus clarkia through whole genome association analysis, whole genome association analysis is performed on the meat percentage of the procambarus clarkia, and the SNP marker remarkably related to the meat percentage character is screened out and comprises one or more of SNP1-SNP10. By detecting the SNP marker, the procambarus clarkii with high growth speed and high meat percentage can be effectively predicted and bred, the breeding efficiency and accuracy of the procambarus clarkii are remarkably improved, a new variety of the procambarus clarkii with high yield and high quality is bred, and the SNP marker has a wide application prospect in the aspect of molecular breeding of the procambarus clarkii with the meat percentage character.

The invention belongs to the technical field of whole-genome gene chips, and particularly relates to a leymus chinensis whole-genome 50K liquid chip and application thereof. According to the leymus chinensis whole genome 50K liquid phase chip, a whole genome re-sequencing technology is utilized, an SNP molecular marker combination of leymus chinensis is determined, and then the leymus chinensis whole genome 50K liquid phase chip is developed. The chip is combined with a high-density SNP probe, realizes high-throughput and high-precision genetic typing of leymus chinensis genomes, and particularly has wide application prospects in the field of molecular breeding.

The invention discloses an intelligent computing platform for animal gene breeding, which adopts RFID electronic ear tags or other biological recognition technologies to carry out individual marking and identity authentication on each breeding pig, collects basic information of the breeding pig in real time, and carries out intelligent computing on the basis of a statistical genetics model in combination with a machine learning algorithm. The method comprises the following steps: collecting phenotypic data and genotypic data, carrying out deep analysis on the collected phenotypic data and genotypic data, calculating a breeding value or a genome breeding value of each breeding pig, designing and applying an optimization algorithm according to a genetic evaluation result, formulating an optimal breeding pig mating plan, and realizing automatic monitoring of pig house environment parameters through an Internet of Things technology. The method comprises the steps of performing intelligent analysis on behaviors and postures of pigs in combination with an AI image recognition technology, developing a matched software system, integrating data analysis results, establishing a database based on a cloud computing architecture, constructing a visual platform containing various charts and reports, and converting complex data into visual and understandable information.

The invention discloses a linked molecular marker of a major QTL (quantitative trait loci) uq2 of a peanut legume singulation character as well as an identification method and application of the linked molecular marker. The molecular marker and a major QTL site uq2 of the peanut legume singulation character are co-localized on a second chromosome of a peanut genome, and the molecular marker comprises molecular markers SNP4 and SNP57. The physical position of the molecular marker SNP4 is the 11591222th base position of the second chromosome of a cultivated peanut genome, and the polymorphism is C / T; the physical position of the molecular marker SNP57 is the 11894442 base position of the second chromosome of the cultivated peanut genome, and the polymorphism is A / T. The linked molecular marker is obtained by using a primer group. The nucleotide sequences of the primer group are respectively as shown in SEQ ID No. 1 to SEQ ID No. 6. The major QTL locus uq2 of the peanut legume constriction character as well as the linked molecular marker and the identification method of the major QTL locus uq2 are suitable for gene localization of the peanut legume constriction character and molecular marker-assisted selective breeding.

The invention provides a molecular marker linked with a wheat stripe rust resistance gene YrZ41-2AL and application of the molecular marker, and belongs to the technical field of molecular markers. The nucleotide sequence of the molecular marker KASP-2AL-2 provided by the invention is as shown in SEQ ID NO. 1, and the polymorphism of the nineteenth base of the nucleotide sequence is T or C. The molecular marker KASP-2AL-2 has the advantages that the nucleotide sequence is shown in SEQ ID NO. The molecular marker KASP-2AL-2 is closely linked with the stripe rust resistant gene YrZ41-2AL on the wheat 2A chromosome, can rapidly and accurately detect the stripe rust resistant gene, and has high application value in the aspects of screening or identifying the wheat stripe rust resistance, cultivating wheat with stripe rust resistance, improving wheat germplasm resources and the like.

The invention belongs to the technical field of biology, and particularly relates to application of a QTL (Quantitative Trait Loci) Qsdss.saas.6A to identification of a wheat SDS (Sodium Dodecyl Sulfate) sedimentation value genotype and an identification kit. The QTL locus Qsdss.saas.6A is located on a short arm of a wheat 6A chromosome, the genetic interval is 28.14 cM to 48.75 cM, and corresponding molecular markers on the two sides of the QTL locus Qsdss.saas.6A are AX-110002278 and AX-111581648. Meanwhile, the invention also provides a KASP primer group, the detection kit prepared from the KASP primer group is applied to detection of the SDS sedimentation value of wheat grains, wheat strains with high SDS sedimentation value can be rapidly screened, and the breeding efficiency is greatly improved; the identification method provided by the invention is simple in steps, reliable and practical, has an important application value in wheat breeding, and has a wide application prospect.

The invention relates to the technical field of plant germplasm resources. The invention provides an SSR-PCR (Simple Sequence Repeat-Polymerase Chain Reaction) primer group for a robinia pseudoacacia genome SSR molecular marker, a robinia pseudoacacia core germplasm fingerprint spectrum obtained by utilizing the primer group and a method for constructing a robinia pseudoacacia core germplasm bank. The SSR-PCR primer group disclosed by the invention is relatively high in polymorphism, good in stability, relatively good in expression in the operation of the SSR molecular marker of the robinia pseudoacacia germplasm genome, and good in screening effect. By adopting the construction method disclosed by the invention, excellent germplasm with special characters can be screened out to construct the fingerprint spectrum of the robinia pseudoacacia core germplasm, a basis is better provided for scientific management, efficient utilization and preservation of robinia pseudoacacia germplasm resources, and meanwhile, the breeding efficiency can be effectively improved.

The invention provides a larimichthys crocea fast-growing character associated SNP marker combination and breeding application, the combination is prepared from a probe for detecting 10 SNP molecular marker combinations of larimichthys crocea fast-growing characters, and information of positions of all SNP molecular markers on chromosomes, alleles with changed sites and reference genes is shown in a table 1. According to the SNP marker combination disclosed by the invention, the optimal large yellow croaker individuals with fast-growing potential and a certain scale can be selected from the breeding offspring in advance for breeding and breed conservation, and the large yellow croaker individuals are bred to the parent without breeding and breed conservation of a large number of offspring seeds in the breeding process, so that the breeding cost can be greatly reduced. The SNP marker combination provided by the invention can solve the problem that non-lethal detection of the growth speed of the large yellow croaker cannot be realized in a traditional breeding mode, and can ensure smooth development of fast-growing character breeding work of the large yellow croaker.

The invention discloses SNP (Single Nucleotide Polymorphism) molecular markers of a Penaeus vannamei HIF-1alpha gene. The SNP molecular markers are respectively positioned at basic groups of the 67th site, the 119th site, the 133rd site, the 172nd site, the 340th site, the 409th site, the 437th site, the 474th site, the 577th site, the 647th site and the 749th site of a nucleotide sequence shown in a sequence 1 in a sequence table. The molecular marker is used as a functional marker of the nitrite-resistant character of the penaeus vannamei for breeding, specifically, genome DNA of muscular tissue of the penaeus vannamei to be detected is extracted, then the obtained genome DNA is used as a template for PCR amplification and purification of a PCR amplification product, then the obtained PCR amplification product is sequenced, and the content of the nitrite-resistant character of the penaeus vannamei is determined. The genotype of the molecular marker is determined, so that dominant genotype individuals are selected for breeding the penaeus vannamei boone, and a penaeus vannamei boone strain with good nitrite-resistant character can be obtained.

The invention belongs to the technical field of plant breeding, and relates to a transgenic potato event ST2400643 and a detection method thereof. The invention provides a nucleic acid molecule for detecting a transgenic potato event ST2400643. The sequence of the nucleic acid molecule is shown as SEQ ID NO: 1-2 or a complementary sequence of the SEQ ID NO: 1-2. Or the sequence of the nucleic acid molecule is shown as SEQ ID NO: 3 or a complementary sequence thereof; the nucleic acid molecule is derived from a transgenic potato event ST2400643, and a potato plant containing the transgenic potato event ST2400643 has been preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC NO.46132. A potato plant comprising the transgenic potato event ST2400643 of the invention has the following advantages: the potato plant can tolerate imidazolinone herbicides; the potato yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking transgenic insertion fragments in a breeding population and a progeny thereof.

The invention discloses a japonica rice amphiphilic hybridization target gene plant screening method based on high-throughput sequencing, and belongs to the technical field of agricultural biology. A high-throughput sequencing technology is utilized, the genetic difference of parent genomes is accurately analyzed, specific molecular markers covering target gene areas are developed, plants containing target genes can be rapidly recognized, the screening efficiency and accuracy are greatly improved, the breeding period is shortened, resources are saved, and the method is suitable for large-scale popularization and application. Through combination of molecular marker detection and phenotypic expression of target characters, in the construction process of hybridization and backcross populations, target genes are fixed generation by generation, stable inheritance of the target characters, joint analysis of genetic types and phenotypes and multi-environment testing are ensured, correlation between target genotypes and the target characters and environmental adaptability of the target genotypes and the target characters are verified, and the target genotypes and the target characters are identified. The stability and reliability of the breeding material are improved, in candidate plant screening and restorer line creation, an excellent hybrid combination is constructed, genotype optimization is realized, and the quality and yield potential of the breeding material are improved.

The invention belongs to the technical field of molecular biology, and particularly relates to a cassava plant height SNP molecular marker, a KASP primer group and application of the cassava plant height SNP molecular marker and the KASP primer group. According to the cassava plant height SNP molecular marker, the allelic variation basic group of the SNP molecular marker site is C / T, and the allelic variation basic group is located at the 3091721 site of a cassava genome 6 # chromosome. The SNP molecular marker is remarkably related to the cassava plant height character, in practical application, the cassava plant height phenotype can be subjected to early-stage auxiliary prediction and identification only by utilizing the KASP primer group to carry out PCR amplification on cassava germplasm leaf sample DNA and detecting a fluorescence signal of the cassava germplasm leaf sample DNA, operation is easy and convenient, cost is low, selection efficiency is high, and the SNP molecular marker can be used for early-stage auxiliary prediction and identification of the cassava germplasm plant height phenotype. Target single plants or a large number of offspring with potential excellent quality can be efficiently screened, the cassava breeding period is shortened, and the breeding efficiency is improved.

The invention discloses an InDel molecular marker primer for identifying soybeans with high palmitic acid content and application of the InDel molecular marker primer. The nucleotide sequence of an upstream primer of the InDel molecular marker primer is as shown in SEQ ID NO.1, and the nucleotide sequence of a downstream primer of the InDel molecular marker primer is as shown in SEQ ID NO.2. Aiming at a QTLqPA21 interval, in combination with amphiphilic re-sequencing data, an Indel site with a difference basic group greater than 15 bp is screened out, and an InDel molecular marker is designed aiming at basic group deletion existing at Chr025018100. According to the InDel molecular marker, an InDel primer sequence is designed, PCR amplification is carried out, a detection result is observed, and the palmitic acid content phenotype is identified. The primer disclosed by the invention can be used for identifying and screening phenotypes of the palmitic acid content of soybeans, and can be used for rapidly, accurately and effectively breeding soybean varieties with high palmitic acid content, so that the breeding process of crops is accelerated, and the breeding efficiency and success rate are improved.

The invention discloses an SV molecular marker related to a corn nitrogen utilization efficiency character and application of the SV molecular marker. The SV molecular marker is located on the 6722060-6724624 basic group of the fifth chromosome of a corn B73 genome with the genome version number of GRAMENE-4. 0, and the sequence of the SV molecular marker is shown as SEQ ID NO.3. The invention further discloses a method for preparing the SV molecular marker related to the corn nitrogen utilization efficiency character and application of the SV molecular marker related to the corn nitrogen utilization efficiency character. The corresponding primers and the detection kit are designed according to the SV molecular marker, so that prediction and screening of the corn nitrogen utilization efficiency character are realized, and a scientific basis is provided for cultivation of corn inbred lines with different nitrogen utilization efficiency types.

The invention belongs to the field of molecular genetic breeding, and particularly relates to a KASP molecular marker method related to peanut aspergillus flavus seed kernel infection resistance and application of the KASP molecular marker method. Through whole genome association analysis, the SNP locus is located at the 133198521th bp position of the peanut B06 chromosome (the reference genome is peanut), the nucleotide sequence polymorphism is C / A (the antisense strand is G / T), and the SNP locus is named as qARIB06. Based on the SNP site, KASP molecular marker primer sequences (SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3) are designed and developed, and the KASP molecular marker primer sequences are used for rapidly and accurately identifying the genotype of a peanut sample.

The utility model relates to the technical field of rice breeding, and discloses a breeding device aiming at rice variety breeding, a box door is clamped on one side of a breeding box, a positioning frame is clamped on the position, close to the breeding box, of one side of the box door, a breeding plate is installed on the upper surface of the positioning frame, a nutrient solution box is arranged on the other side of the breeding box, and the nutrient solution box is arranged on the other side of the breeding box. A heating plate is arranged on the lower surface of the nutrient solution box, a liquid outlet pipe is arranged on the upper surface of the nutrient solution box in a penetrating mode, one side of the liquid outlet pipe is communicated with a connecting pipe, atomizing nozzles are symmetrically arranged on the lower surface of the connecting pipe in the breeding box, and a water return pipe is further arranged at the position, close to the breeding box, of the upper surface of the nutrient solution box. A nutrient solution in a nutrient solution box can enter a connecting pipe through a liquid outlet pipe under the work of a water pump, finally, the nutrient solution is sprayed outwards through an atomizing spray head to breed rice on the breeding plate, and meanwhile, the nutrient solution is recycled through a water return pipe, so that the cost is reduced.

The invention provides an SNP (Single Nucleotide Polymorphism) site which is located at the 296th site of a MeGRXC4 gene coding region, the basic group is G or C, and the sequence of the MeGRXC4 gene coding region is as shown in SEQ ID NO: 1 or SEQ ID NO: 2. The SNP located at the 296th site of the MeGRXC4 nucleotide sequence is identified to be closely associated with the drought resistance of the cassava from the big data level for the first time, detection is facilitated, accuracy and reliability are achieved, operation is convenient, the drought resistance of the cassava is identified through the SNP sites, the accuracy is high, the method is not affected by the growth period of the cassava, selection can be conducted in the seedling period, the breeding efficiency is remarkably improved, and the method is suitable for large-scale popularization and application. In addition, when the basic group of the SNP site is G, MeGRXC4 can significantly improve the drought resistance of cassava, and the invention has important significance for revealing the relationship between allele selection and drought resistance character formation in the domestication process of cultivated cassava, and provides gene resources and scientific basis for cultivating new varieties of drought-resistant high-yield cassava by using key genes.

The invention discloses a pea whole genome 20K SNP (Single Nucleotide Polymorphism) liquid phase breeding chip and application thereof, the liquid phase breeding chip comprises 21 and 659 SNP loci on a pea whole genome, the SNP loci comprise published 235 reliable functional loci obviously related to 57 characters, and examples for pea sample genetic typing are provided. The chip can be widely applied to important character analysis, germplasm resource genetic diversity analysis, variety identification analysis, auxiliary selective breeding, population structure analysis, heredity and evolution analysis, genetic relationship identification, whole genome association analysis or genome selection of peas, and is helpful for improving the pea breeding efficiency; the innovation and improvement of pea varieties are promoted.

The invention discloses a method for creating melatonin-rich soybeans by utilizing synthetic regulatory elements and application of the melatonin-rich soybeans. According to the invention, a soybean standard line Williams 82 (W82) is used as a receptor, melatonin pathway genes COMT and SNAT are optimized through codons to synthesize regulation elements to efficiently and accurately activate expression of COMT and SNAT, and specific accumulation in soybean seeds is realized, so that a new soybean material rich in melatonin in the seeds is created. Compared with a control receptor, the melatonin content of the modified soybean is increased by more than 30 times. The method for efficiently producing the melatonin by synthesizing the regulatory element has an important value for increasing the content of the melatonin, provides a new thought and tool for improving the quality of soybeans and crops, is beneficial for supplementing high-value active substances in daily diet, and has a wide application prospect. The method has strong application potential in the aspect of assisting biosynthesis of plant-derived natural products.

The invention discloses an introduced-variety pig 10K SNP liquid chip based on targeted capture sequencing and application, and belongs to the technical field of biomolecular breeding. Target SNP sites are collected, selected and filtered, an SNP site set with comprehensive performance is screened out, a probe is designed on the basis of the SNP site set, the introduced variety pig 10K SNP liquid phase chip is further prepared, the SNP sites of the chip have the characteristics of uniformity, polymorphism, compatibility, stability and functionality, pig genomes can be covered to the maximum extent, and the detection sensitivity is high. Meanwhile, the selection of pig genomes can be greatly reduced, especially the cost of early selection of variety pigs is reduced, in addition, the selection efficiency and filling accuracy of chip sites filled to more than 50K can be ensured, and the requirements of subsequent variety identification, lineage analysis, genetic relationship identification, genome selective breeding and whole genome association analysis are met.

The invention belongs to the technical field of biology, and particularly relates to an Indel molecular marker closely linked with the shape of a fleshy root of a radish, a primer, a kit and a method for amplifying the Indel molecular marker and application of the Indel molecular marker. The first Indel marker and the second Indel marker are located in the 19.6-20.1 Mb interval of the chromosome 7 of the radish genome. The nucleotide sequence of the first Indel marker is deleted by 4bp in the extreme long radish compared with the nucleotide sequence of the first Indel marker in the extreme round radish; the nucleotide sequence of the second Indel marker is deleted by 25bp in the extreme long radish compared with the nucleotide sequence of the second Indel marker in the extreme round radish. Quantitative character sites for controlling the shape of the fleshy root of the radish are excavated, the molecular marker closely related to the root shape is developed, and materials with excellent root shape characters can be identified and selected more quickly and accurately, so that the breeding process is accelerated, and the breeding efficiency is improved.

The invention discloses an MBTD1 gene molecular marker related to the body weight and body size of a goose and application of the MBTD1 gene molecular marker. The molecular marker is located at the 6290111th site of the 18th chromosome of the goose, and the site polymorphism is C or A. The polymorphic site is remarkably related to the body weight and body size characters of the geese, can accurately judge the final change of the body weight and body size in the future development process of the geese, can be used for early screening of the body weight and body size characters of the geese, greatly reduces the screening cost, accelerates the genetic breeding process, effectively improves the breeding efficiency, and has broad application prospects. The method has high economic application value and scientific research value.

The invention belongs to the technical field of breeding of cotton hybrids, and discloses a breeding method and an intelligent breeding system of high-temperature-resistant high-quality cotton hybrids, which improve the high-temperature resistance: breeding a cotton variety with remarkably improved high-temperature resistance through a gene screening and introducing technology, and reducing the negative influence of high temperature on cotton growth. An intelligent breeding system and a high-throughput genome sequencing technology are applied, so that the hybridization efficiency is remarkably improved, and the breeding period is shortened. By using the intelligent breeding platform, the automation and intelligence of the breeding process can be realized, the manual operation is reduced, and the breeding cost is reduced. Through multi-environment tests and data analysis, the bred cotton variety has better environmental adaptability and can be popularized and applied in different areas, and the cotton yield and quality are improved.

The invention belongs to the technical field of biology, and particularly relates to a barley grain weight related gene HvTGW1s and application thereof. The invention discloses a barley grain weight related gene HvTGW1s, the base sequence of the barley grain weight related gene HvTGW1s is shown as SEQ ID NO: 1, and the barley grain weight related gene HvTGW1s has the function of increasing the thousand grain weight, grain width and grain length of barley. The invention further discloses the KASP marker associated with the barley yield character and application of the KASP marker in barley yield assisted selective breeding. The invention further discloses a kit for assisting in identifying the barley yield. The method is applied to barley yield assisted selection breeding, the breeding efficiency can be effectively improved, and the breeding process is accelerated.

The invention relates to the field of genes, in particular to molecular markers for judging flowering time of brassica napus L. The molecular markers are Indel-282, Indel-326 and Indel-353 respectively and are located on an A02 chromosome of the brassica napus L.. The molecular marker has high specificity and stability, and is significantly related to the flowering time. The markers show high specificity and stability in brassica napus plants under different genetic backgrounds. By detecting the molecular markers through PCR amplification and electrophoresis, the flowering time of different plants can be quickly and accurately identified in the early stage of breeding. Therefore, the flowering time of the plant can be determined in the early stage, so that the breeding efficiency is improved. The molecular markers can be used for molecular marker-assisted selective breeding, and plants with early flowering or late flowering characteristics can be quickly screened by detecting the markers, so that the breeding process of excellent varieties is accelerated, and the functions and regulation mechanisms of related genes are further disclosed.

The utility model discloses a stem cell culture device which comprises a box body, sliding grooves, culture drawers, inserting rods and heat insulation plates, the four heat insulation plates are fixedly installed on the inner side of the box body in a vertical array mode, culture bins are formed in the top end of the inner side of the box body and between every two adjacent heat insulation plates, and the sliding grooves are formed in the inner side walls of the culture bins; the sliding grooves of every two adjacent culture bins are perpendicular to each other, sliding strips are fixedly installed on the left side wall and the right side wall of each culture drawer, a handle is fixedly installed in the center of the outer side wall of each culture drawer, the sliding strips are transversely and slidably connected with the inner sides of the sliding grooves, and a plurality of fixing holes are evenly formed in the bottom of the inner side of each culture drawer. The side wall of the culture bin is communicated with a ventilation pipe, and the other end of the ventilation pipe is communicated with the ventilation chamber; according to the stem cell culture device, the independent culture drawer is arranged for taking and placing, so that culture of other stem cells is not delayed, and the culture efficiency of the device is improved.