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344results about How to "Improve breeding efficiency" patented technology

Breed conservation, screening and breed production method in tilapia complete set lines as well as between two lines of complete sets

The invention relates to a breed conservation, screening and breed production method in tilapia complete set lines, comprising the following steps of: construction of complete set line base populations rich in hereditary variation, establishment of operation procedures of family production and management in the complete set lines, marking of individuals in the complete set lines, genetic evaluation analysis of important production traits and establishment of individual mating selection programs in the complete set lines. The invention also provides a hybrid breed production method between twolines of complete sets, which is established by utilizing the method. The method disclosed by the invention has the characteristics of high pertinence, high selection breeding efficiency, capability of effectively preventing parent inbreeding depression of the complete set lines and the like; the harvest weight of the hybrid fine breed of tilapia is averagely increased by more than 30% than that of control groups; and the culture survival rate is also obviously higher than that of the control groups. According to the invention, a new technical approach is developed for cultivation of fish fine breeds; particularly a new selection breeding method is applied in the breed conservation, the screening and the breed production of the complete set lines in fish hybrid breeding; and the method isalso suitable for being popularized and applied in all cultured fishes and has broad popularization and application prospects.

Molecular label from high-quality variety Xinluzao No.24 and relevant with cotton fiber strength

The invention discloses a molecular label linked with a major gene of a high-strength cotton fiber, which is obtained by the following method: taking the Xinluzao No.24 as a male parent, taking Lunianyan No.28 and Ji cotton 516 as female parents; respectively constructing two segregation populations of F2 and F2:3; carrying out polymorphism screening between parents (Lunianyan 28 and Xinluzao 24)by utilizing an SSR (simple sequence repeat) primer; constructing a genetic linkage map by utilizing F2; carrying out two generation quantitative trait locus (QTL); selecting an SSR label linked withthe high-strength cotton fiber to analyze the DNA polymorphism of the parent Xinluzao 24 and the Ji cotton 516; and constructing the genetic linkage map and positioning QTL; the fiber strength major QTL (qFS-62) can be stably detected in two populations and generations, and is positioned between the label interval of DPL0757-DPL0920 of chromosome c7; a synergtic gene is from the parent Xinluzao No.24; and the linking label of qFS-6-2 is DPL0757130, DPL0852170 and DPL0920130. By utilizing the label provided by the invention, molecule label assisted selection is carried out, thus improving the selection efficiency of the high-strength cotton fibre.

Breeding method for multi-resistance flue-cured tobacco hybrid

The invention provides a breeding method for a multi-resistance flue-cured tobacco hybrid. The breeding method for the multi-resistance flue-cured tobacco hybrid comprises the steps of firstly using MSG28 as a female parent, using high-quality, widely applicable and blackleg-resistant varieties which are sensitive to TMV and weather fleck and are used for production as recurrent parents, performing eight generations of backcrossing to breed male sterile lines, using fertile materials which are resistant to blackleg and highly resistant to TMV and weather fleck as original resources, performing six generations of selfing purification to breed selfing pure lines with common or better curing characteristics, then using the bred male sterile lines as female parents and the selfing pure lines as male parents to breed hybrid combinations, respectively evaluating resistance to different diseases and economic performance by using land parcels or areas seriously attacked by different diseases and normal land parcels in producing areas, and finally performing 2-3 years of multi-point tests for quality and adaptability evaluation to the hybrid combinations which are simultaneously resistant to different diseases and have good economic performance. By adopting the method, the aggregation of multiple characters such as high quality, simultaneous resistance to foliage diseases and root and stalk diseases and good adaptability can be realized, the breeding efficiency is improved.

SSCP marker closely linked with major wheat scab resistance QTL and application thereof

The invention relates to a single strand conformation polymorphism (SSCP) marker closely linked with a major wheat scab resistance quantitative trait locus (QTL). The SSCP marker is characterized in that: scab resistance candidate genes of a scab resistance QTL in a 3BS area of a wheat variety or strain resisting scab is PCR amplified by using a primer; after denaturalization, a PCR-amplified product has different single strand conformation; and the SSCP marker closely linked with the major wheat scab resistance QTL is established for detecting a genotype of the wheat variety or strain during breeding. The SSCP marker has the advantages of: overcoming the disadvantages that the wheat scab resistance screening can only be authenticated in a flowering period and is easily influenced by the environment in conventional breeding, predicting and screening wheat plants with the scab resistance by detecting a molecular marker at a seedling stage, eliminating disease plants, reducing waste of labor and materials and improving the breeding efficiency. Compared with an ABI DNA sequence analysis meter-based marker, the SSCP marker closely linked with the major wheat scab resistance QTL has the advantages of simple and convenient operation, low cost and same sensitivity.

Method for breeding raphanus sativus L. CMS (genic male sterility) lines by using marker assisted selection

The invention relates to a method for breeding raphanus sativus L. CMS (genic male sterility) lines by using marker assisted selection, which belongs to the technical field of crop breeding and is used for efficient breeding of excellent male sterility lines in raphanus sativus L. hybrid advantage utilization. The invention adopts the technical scheme that: the method comprises the following steps of: screening inbred lines with excellent traits and stable fertility; selecting specific primers NAUmsF4 and NAUmsR4 to perform PCR amplification on the screened inbred lines, and determining that the inbred lines capable of amplifying 1069bp specific strips cannot be used as candidate maintenance lines; performing restored gene functional marker analysis on the inbred lines incapable of amplifying the specific strips, and screening the inbred lines capable of serving as the candidate maintenance lines; and performing cross and back cross of 4 to 5 generations on the original sterile lines and the identified candidate maintenance lines to obtain new sterile lines and corresponding maintenance lines with excellent traits. The method for quickly screening the candidate maintenance lines by using molecular makers is not affected by a growth period and an environment, can remarkably improve the breeding efficiency of the raphanus sativus L. CMS lines, and accelerates the breeding progress of excellent F1 hybrids.

Floating seedling raising method for sweet potato true seeds

The invention relates to a floating seedling raising method for sweet potato true seeds, and belongs to the technical field of sweet potato true seed seedling raising.The method specifically comprises the steps that 1, a floating seedling raising pond is built and filled with water; 2, 200-pore foam floating discs are selected and filled with a seedling raising matrix with the water content of 60%; 3, the germinated sweet potato true seeds which are treated through 98% concentrated sulfuric acid and subjected to hot water and agentia soaking treatment are selected to be seeded and covered with soil, the soil surface is leveled, and the seeds are put into the seedling raising pond to be cultured; 4, water is supplemented for once every about 15 days during culturing; 5, seeding raising fertilizer is applied for the first time 3-5 days after seedlings are neat, the application amount is 15 g/disc, the concentration of nitrogen is kept to be 300 mg/L, and top dressing is performed for once every 7 days; 6, the seedlings reach the strong seeding standard after the seeds are seeded for 30-40 days and can be transplanted together with the soil.According to the floating seedling raising technique, quick and neat seedling emergence of the seeds can be promoted, the seedling emergence rate can reach 100%, the seedlings can be neat after emerging for 3-4 days, growth of the true seedlings can be kept consistent, the seedlings are strong, the root systems are developed, the root-top ratio is high, the plant height is appropriate, and time and labor are saved in the whole process.

SSR (simple sequence repeat) markers on No.3 chromosome, closely linked to RBSDV (rice black-streaked dwarf virus) resistant QTL (quantitative trait locus) and application thereof

The invention discloses molecular markers closely linked to an RBSDV (rice black-streaked dwarf virus) major QTL (quantitative trait locus). The essence of the invention is that according to the linkage and segregation rules, the F2 population of the hybrid generation of Tetep and RBSDV susceptible varieties is taken as the mapping population and the SSR (simple sequence repeat) markers RM5626 and RM7097 on the No.3 chromosome, which are closely linked to the RBSDV resistant major QTL are obtained through analysis of linkage between the results of evaluation of disease resistance and the SSR marker data. Whether a plant has resistance to RBSDV can be judged by detecting the SSR marker banding pattern of the single rice plant of the hybrid combination generation of Tetep and derived varieties (lines) thereof and the RBSDV susceptible varieties. After the SSR markers are applied to assistant selection breeding of the RBSDV resistant varieties of Tetep and derived varieties (lines) thereof/RBSDV susceptible varieties, the defects of poor stability and repeatability of RBSDV resistance evaluation, time and labor waste, high technical threshold and the like in conventional breeding can be overcome and the results can conduce to simplifying the selection method and improving the breeding efficiency and then accelerating the breeding process of RBSDV resistant varieties.

A kind of breeding method of radish rich in anthocyanins

The invention relates to a crop breeding method, in particular to a radish breeding method, belonging to the technical field of plant breeding and cultivation. The breeding method is characterized in that Kexing red-core and red-skin radishes are taken as female parents, wherein the Kexing red-core and red-skin radishes come from Sichuan province, have a moderate maturity period and upright leaf bunches, contain high-content anthocyanin, are high-yield, disease-resistant, red-skinned and have red flesh and long cylindrical flesh roots; Hengfeng transparent-core red radishes as male parents, wherein the Hengfeng transparent-core red radishes come from Chongqing, have a moderate maturity period and half upright leaf bunches, contain high-content anthocyanin, are heat-resistant and red-skinned and have red flesh, long cylindrical flesh roots and slightly larger lower parts; and the female parents are hybridized with the male parents to obtain intervarietal hybrids F1, and the intervarietal hybrids F1 are continuously self-bred to obtain a new high-quality, high-yield, high-anthocyanin-content and disease-resistant red-skin and red-core radish variety, i.e. Yanzhi No.1. The radish breeding method has the beneficial effects that the breeding efficiency is high, the obtained products are suitable for the extraction processing of anthocyanin and the problem existing in the raw material supply in the anthocyanin extraction processing industry is solved.

Novel method for artificially simulating crop growth shade and humidity environment

The invention discloses a method of a novel technology for artificially simulating a crop growth shade and humidity environment and particularly relates to a novel method for artificially simulating the crop growth shade and humidity environment. According to the method, on the basis of a steel frame greenhouse, power-driven sunshade nets are installed at the top and on the two sides of the greenhouse for controlling illumination in the greenhouse, a spraying system composed of a PPR pipe and a spray nozzle is erected inside the greenhouse for controlling humidity and temperature in the greenhouse, ventilation systems composed of gauzes and power-driven exhaust fans are installed in the middle portions of the two sides of the greenhouse and used for controlling the temperature and the carbon dioxide concentration in the greenhouse, and according to comparison of temperature, humidity and other meteorological data on agricultural environment monitors installed inside and outside the greenhouse, light controlling, humidifying, cooling and ventilating measures are taken, so that the shade and humidity environment which is remarkably different relative to local natural environment conditions is formed. The step that according to the comparison of the temperature, humidity and other meteorological data on the two agricultural environment monitors inside and outside the greenhouse, the corresponding light controlling, humidifying, cooling and ventilating procedures are adopted is the key. The shade and humidity environment is simulated through the method, and the method has the advantages of being wide in geographical adaptability and suitable for different types of crops needing shade-tolerance and humidity-resistance screening and improving shade-tolerance and humidity-resistance breeding efficiency.
Owner:重庆三峡农业科学院 +8
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