380 results about "Cytoplasmic male sterility" patented technology

This disclosure concerns a plant of the genus, Brassica, or parts thereof, which comprise one or more traits selected from the group consisting of high oleic acid content, low linolenic acid content, increased herbicide resistance, restorer of cytoplasmic male sterility, and increased clubroot disease (Plasmodiophora brassicae) resistance, compared to a wild-type plant of the same species. This disclosure further relates to wild-type and mutant alleles of genes involved in these traits, molecular markers linked thereto, and methods of their use.

The present invention includes nuclear fertility restorer genes, proteins encoded by those genes and transgenic plants and plant cells containing those genes. More particularly, the nuclear fertility restorer genes can be used to restore fertility in cytoplasmic male-sterile plants such as Brassica napus. Preferably, the nuclear fertility restorer genes are used with the Ogura (ogu) CMS system in Brassica napus.

The present invention includes nuclear fertility restorer genes, proteins encoded by those genes and transgenic plants and plant cells containing those genes. More particularly, the nuclear fertility restorer genes can be used to restore fertility in cytoplasmic male-sterile plants such as Brassica napus. Preferably, the nuclear fertility restorer genes are used with the Ogura (ogu) CMS system in Brassica napus.

The invention relates to a method for selecting male sterility line of rape cytoplasm, wherein the invention comprises that: selecting wide mustard sterility male, using mustard rape as hold line; crossing and molecule mark to assist the selection, to obtain the mustard rape cytoplasm male sterility line and hold line with stable generation; cultivating said sterility line to obtain usable mustard rape cytoplasm male sterility line.

The invention discloses an onion cytoplasm male sterility SCAR marker, wherein the length of the specific fragment of the SCAR marker is 339bp, and the nucleotide sequence thereof is shown as SEQ ID No.2. The invention further discloses a primer of the SCAR maker, wherein the forward primer is Zt339-u:5'-GTTCTCAAGCAGATTCCGCAC-3', and the reverse primer is Zt339-d:5'-AGGACCAAACCGAGAGTGAGC-3'. Quick and accurate selection for onion cytoplasm fertility can be realized by using the obtained SCAR marker, thereby quickening the seed selection for new breed strain of onion cytoplasm male sterility, and establishing novel onion coenospecies parent selection system; first-filial generation breeds with independent intellectual property are selected and bred, thereby solving the difficult problems that conventional seeds are mainly used in the current production in China and large sums of foreign exchange is used for buying foreign coenospecies.

The present invention relates to methods for enhancement of naturally occurring cytoplastic male sterility and for restoration of male fertility and uses thereof in hybrid crop production. There is also disclosed a method for restoration of male fertility to cytoplasmic male sterile plants; which comprises the steps of: a) introducing into the nucleus of a plant cell a gene construct essentially consisting of a sequence encoding a mitochondrial transit peptide fused upstream of and in frame with an edited form of a normal mitochondrial gene that is co-transcribed with an usual CMS-associated mitochondrial gene; b) selecting for plant cells that have acquired the gene construct in step a); and c) inducing regeneration of selected plant cells to produce a mature plant.

The purpose of the present invention is to provide a method for providing and inhibiting the rice fertility to the rice BT type cytoplasmic male sterility, and discerning the presence of the rice restorer gene. The present invention employs a nucleic acid having the base sequence of SEQ ID NO.27, or a nucleic acid having a base sequence which is identical to at least 70% of the base sequence of SEQ ID NO.27, and which functions to restore fertility. Alternatively, a nucleic acid having the base sequence of bases 38538-54123 of SEQ ID NO.27, or having a base sequence which is identical to at least 70% of the base sequence of bases 38538-54123 of SEQ ID NO.27, and which functions to restore fertility, is used.

The invention relates to a breeding method of hybrid cabbage, in particular to a simplified breeding method for preparing hybrid cabbage by using cytoplasmic male sterile lines, which belongs to the technical field of plant culture. The invention directly adopts the cabbage varieties of Zhonggan No.22 and Hugan No.2 prepared by cytoplasmic male sterile lines sold in the market as maternal plants, and adopts cabbage inbred lines (with the same ball shape as the maternal plants, the same type of leaf ball mature performance as the maternal plants and stable botanical characters) as pollen suppliers for preparing good coenospecies. The invention has the advantages that when the advantages of using the cytoplasmic male sterile lines for preparing the cabbage coenospecies are maintained, good cabbage inbred lines do not need to be used as recurrent parents for fifth to seventh generation backcrossing and transformation with male sterilant materials, the labor is saved, the cost is saved, the breeding time is greatly shortened, and in addition, the hybrid vigour parts of the original varieties can be maintained into the new hybrid varieties.

The invention relates to a breeding and cultivating method for a plant, in particular relates to a breeding and cultivating method for loose purple cauliflowers, and belongs to the technical field of plant cultivation. The breeding and cultivating method comprises the steps of hybridizing a loose purple cauliflower breeding material with a semi-compact purple cauliflower breeding material to obtain seeds F1, and selfing single strains with high comprehensive agronomic characters after the seeds F1 are sowed to obtain seeds F2; performing microspore culture on single loose purple cauliflowers through flower buds suitable for the microspore culture from the mononuclear edging stage to the double-core early stage to grow a large number of normal regenerated plants, selfing excellent DH plants of the loose purple cauliflowers to obtain a loose purple cauliflower excellent DH strain 03-12 serving as a recurrent parent which is hybridized with a cauliflower cytoplasmic male sterility source material CMS05-6, performing five generations of backcross transformation and selection to form a cytoplasmic male sterile line CMS08-2, and hybridizing the DH strain serving as a parent with the excellent cytoplasmic male sterile line CMS08-2.

The invention discloses a transferring method for head cabbage cytoplasm male sterile line characterized by that, wild cabbage self-bred lines are utilized for 5-7 generation backcrossing with cabbage cytoplasmic protein sterility source. The advantages of the invention include stabilized male sterility, high sterility strain rate and sterility degree. The crossing rate of the obtained hybrid in strictly isolated area can be as high as 100%.

The invention relates to a breeding method and application of a cytoplasmic male sterility restoring line of brassica napus rapeseed and radish. The method comprises the following steps of: choosing rapeseed S1628 with the preservation number of CCTCC NO: P201203, performing continuous inbreeding directional selection for six years to breed an excellent, high-oil and stable inbred line; using the cytoplasmic male sterility restoring line of the brassica napus rapeseed and radish as a female parent and using the bred excellent and stable inbred line as a male parent to select out strains comprising radish cytoplasmic male sterility restoring genes from large amounts of filial generations; using the strains comprising the radish cytoplasmic male sterility restoring genes as the female parent and using Zhongshuang No.2, Huashuang No.3, Huyou 17, Zunyou No.1 and Zheyou 758 of double-low winter rapeseed varieties in China as recurrent male parents to hybridize, performing the continuous inbreeding directional selection for six years to breed the cytoplasmic male sterility restoring line of the double-low and high-oil brassica napus rapeseed and radish. The restoring line has been crossed with hybrid varieties. A gap of producing the hybrid varieties by the cytoplasmic male sterility restoring line of the double-low radish in China is filled, and the application prospect is broad.

This disclosure concerns high-resolution mapping and candidate gene cloning of Rf4, a maize restorer of fertility gene that restores fertility to C-type cytoplasmic male sterility. The disclosure also relates to molecular markers that are tightly-linked to, or reside within, the Rf4 gene. In some embodiments, methods are provided whereby hybrid seeds may be produced from crosses of a male plant comprising nucleic acid molecular markers that are linked to or that reside within the Rf4 gene and a female plant carrying C-type CMS.

The invention discloses a breeding method for a hybrid rice, particularly relating to a method of getting the cytoplasm male sterility three lines from the photo temperature sensing nucleon sterility line; comprising: choosing the photo temperature sensing nucleon sterility line and high quality rice cytoplasm male sterility keeping line for crossing, planting the filial generation in the high temperature and long day to charge off the photo temperature sensing nucleon sterility genes, separating the cytoplasm male sterility recovery line from the filial generation through the test-mating of cytoplasm sterility line, getting the rice cytoplasm male sterility line and recovery line respectively by carrying out the backcross and auto-copulation at the same time. In this method, the introducing the sound characteristics of the photo and temperature sensing nucleon sterility line such as the straight strain and high chapiter exposing rate to the keeping line, promotes the improvement of the cytoplasm male sterility line and establishes a mew way of generating the cytoplasm male sterility line, and at the same time overcomes the shortage of fertility of the rice photo and temperature sensing sterility line easy to be influenced by the light and temperature environment, which is favorable to increase the hybrid rice seed purity and the seed production.

The invention belongs to the technical field of rape molecular breeding, and specifically relates to a breeding method of cabbage type rape woad oil cytoplasm male sterile line. The invention also relates to a protoplast fusion technology to recombinate the cytoplasm and nuclear genome of cabbage type rape-woad so as to establish cytoplasmic male sterility of recombinant cytoplasm. The breeding process comprises the following steps: obtaining the leaf meat protoplast of cabbage type rape-woad through separation and extraction, obtaining the protoplast fused somatic cell hybrid of cabbage type rape-woad through a cell fusion method; taking the obtained somatic cell hybrid of cabbage type rape-woad and leaf meat protoplast cell hybrid of cabbage type rape-woad as the female parent, taking cabbage type rape as the male parent, carrying out multi-generation backcross, and obtaining cytoplasmic male sterile line with carpelloid stamen; wherein the mitochondrion gene PCR amplification verifies that mitochondrial genome carries out recombination during the fusion process, thus the nucleoplasm becomes uneven, and the sterile character appears.

The invention discloses a cabbage type rape Ogura cytoplasmic male sterility (OGU CMS) restorer and an obtained derivative restorer material after the cabbage type rape Ogu CMS restorer is transferred, wherein the preservation number of the Chinese microbial culture preservation management committee center for general microbiology is CGMCC No. 6001, and the plant name is Ogu CMS restorer CLR650. A transformation method of the cabbage type rape OgU CMS restorer comprises the following steps: using a cruciferae resource containing Ogu CMS exogenous restoring genes as a restoring gene donor parent, utilizing a grafting technology to overcome distant hybridization reproductive dysfunction, leading intergeneric radish restoring genes into cabbage type rapes, continuously culturing fertile plants with chlorosis, and obtaining the restorer CLR650 which can be fertile in selfing and test-crossing after screening, selfing and backcross screening for multiple generations. The restorer material is low in cost, good in safety and capable of being applied to a cabbage type rape Ogu CMS pollination system.

The present invention provides the nucleotide sequence and encoded amino acid polypeptide sequence of one cytoplamis male sterile restoring gene OsRf1 originating from rice. The gene is member of PPR gene family, and is one constitutive expression gene. The present invention also relates to the application of utilizing the gene in transforming rice and other plant breeding restorer and the application of the molecular label produced based on the gene sequence in breeding.

The invention belongs to the technical field of rape breeding, and discloses a selection breeding method for a Brassica napus-turnip cabbage cytoplasmic male sterility line (CMS). The method is characterized in that an artificially-bred turnip cabbage allotetraploid as a female parent is hybridized with a Brassica napus variety as a male parent, male sterile plants are isolated from the progeny of hybrid selfing, and a Brassica napus-turnip cabbage cytoplasmic male sterility line with genetic stability is obtained by successive backcross breeding. Restorer line and maintainer line relationship and molecular biological methods are employed to prove genetically and on DNA level respectively that the Brassica napus-turnip cabbage cytoplasmic male sterility line has a sterile cytoplasmic type different from those of Polima cytoplasmic male sterility (pol CMS), turnip cytoplasmic male sterility (ogu CMS, kos CMS) and Brassica juncea cytoplasmic male sterility (hau CMS). The invention adds a new rape cytoplasmic male sterility type for China and the rest of the world.

The invention relates to the field of biotechnology-assisted seed breeding, and particularly discloses a CAPS molecular marker primer for detecting CMS (cytoplasmic male sterility) restoring genes ofcapsicum annuum and application of the CAPS molecular marker primer. CAPS molecular markers are tightly linked with the CMS restoring genes of the capsicum annuum and are M1 markers. The CAPS molecular marker primer and the application have the advantages that detection can be facilitated by the CAPS molecular markers, stable and reliable results can be obtained, the CAPS molecular markers are co-dominant markers, and accordingly sterility restoring genes of capsicum annuum strains can be detected on a large scale only by means of simple conventional experiment operation such as hereditary substance extraction, PCR (polymerase chain reaction), restriction incision enzyme digestion and routine agarose electrophoresis; the selective breeding ranges of restoring line materials for the capsicum annuum can be expanded by development and application of the CAPS molecular markers, the seed breeding efficiency can be improved, seed breeding progresses can be accelerated, and the CAPS molecularmarker primer and the application have important significance in molecular marker-assisted seed breeding for CMS three-line matched seed production and restoring line materials for the capsicum annuum.

The invention relates to a method for breeding kenaf H040 cytoplasmic male sterile line, and belongs to the technical field of breeding of crop male sterile lines. The method takes kenaf wild species H040 or filial generation inherited with cytoplasm of the kenaf wild species H040 as female parent, and takes UG 93 cytoplasmic male sterile maintainer line or kenaf common culture variety as male parent; the female parent and the male parent carry out saturated backcross; the H040 cytoplasmic male sterile line, which is similar to the male parent and is orderly and consistent and exhibits male sterility, is selected and bred; and the backcross female parent is the male sterile maintainer line. The method provides new source of the cytoplasm for the breeding of the kenaf male sterile line, and can combine hybridized combination with comparative advantages.

The invention discloses an Allium cepa cytoplasmic male sterile SCAR marker. Specifically, the specific fragment of the SCAR marker is 2175bp long, and the nucleotide sequence is shown as SEQ ID No.2; a common fragment is 1053bp long, and the nucleotide sequence is shown as SEQ ID No.3. The invention also discloses primers of the SCAR marker, and the primers include a forward primer and a reverse primer respectively shown as SEQ ID No.4 and SEQ ID No.5. The cytoplasmic type of the Allium cepa material can be rapidly and accurately judged by the SCAR molecular marker and the primers involved in the invention, misclassification caused by experimental misoperation and invalid amplification of PCR can be avoided, breeding of Allium cepa cytoplasmic male sterile new lines can be accelerated, and a novel Allium cepa hybrid parent selection system is established.

The invention relates to a breeding method for a plant, in particular to a breeding method for broccoli, belonging to the technical field of plant cultivation. The breeding method comprises the following steps of: performing continuous selfing and directional selection for at least five generations through collected broccoli material to obtain a self compatible line of the broccoli to serve as a transformation male parent to be hybridized with a cytoplasmic male sterile material; breeding a cytoplasmic male sterile line with superior comprehensive performance and stable genetic character through backcross transformation of five generations; then hybridizing the cytoplasmic male sterile line with stable genetic character with the superior self compatible line of the broccoli; and performing field repeated identification and screening to obtain superior hybridized combined variety. According to the method, an optimal growth and development environment for the broccoli is created though low-temperature treatment of seeds in coordination with related facilities and equipment; double blossom and seed setting are realized in one year; the breeding progress can be quickened; and the seed quality is improved.

The invention discloses a preparation method and an application of a molecular marker of a rape male sterility restoring gene. The preparation method comprises the following steps of A, designing degenerate primers according to PPR gene conservative amino acid sites, B, extracting a lamina DNA of a rape cytoplasm male sterility restoring line through a cetyltrimethyl ammonium bromide (CTAB) method and magnifying the lamina DNA through a touchdown polymerase chain reaction (PCR) process to obtain a specific restoring gene candidate segment, C, carrying out recovery, conversion, sequencing and sequence analysis processes on PCR products, D, carrying out 5 ' RACE and 3 ' RACE processes and splicing a sequencing result to obtain a full-length cDNA sequence, and E, designing specific primers in 5 ' UTR and 3 ' UTR zones of the full-length cDNA sequence according to the full-length cDNA sequence to obtain a molecular marker. The invention also discloses an application of a restoring gene molecular marker in hybrid rape parent breeding. The preparation method has the advantages of improvements of a speed of restorer seeding and an efficiency of verification, simple and easy method, good operability, rapid identification speed of restoring genes, great reduction of necessary workload of test cross and progeny planting observation in a restorative verification process, and improvement of an efficiency of hybrid seeding.

The present invention relates to crossbreeding, back crossing, etc. with temperature sensitive polCMS as female parent and selfing incompatible line capable of maintaining its incompatibility as male parent to shift the selfing incompatibility gene into the temperature sensitive polCMS and breeding cytoplasmic male sterility+ selfing incompatible line selectively from the offspring with high temperature sterility and low temperature fine powder selfing incompatibility; top crossing and back crossing of the line with available rape variety and screening to breed maintainer line and restoring line; maintaining the sterile and selfing incompatibility of the cytoplasmic male sterility+ selfing incompatible line with the maintainer line and restoring the bearing capacity of the cytoplasmic male sterility+ selfing incompatible line with the restoring line to form the matched three lines. The present invention has low breeding cost and risk, high hybrid purity and high heterosis.

A directional culture method for the restoring line of three-line hybridized rice includes such steps as hybridization between the three-line hybridized rice as female parent and the excellent rice as recurrent male parent, and backcrossing multiple times. Its advantages are high speed and efficiency, and excellent characteristics of resultant cross rice.

The invention belongs to the field of plant new species breeding technique, specifically to a method of transferring citrus cytoplasm male sterility trait, which is characterized in that based on the phenomenon that the citrus cell symmetrical amalgamation can regenerate diploid diachyma parent-type cybrid and its constituting features at molecule level, the cytoplasm male sterility trait controlled by the mitochondrial genome is transferred by amalgamation compounding matching and the cybrid obtained by culturing, thereby improving the diploid nuclear or multi-core citrus breeds. The invention discloses the symmetric amalgamation between cytoplasm male sterility Wenzhou Satsuma and Chinese characteristic nuclear or multi-core citrus, successfully invents a batch of diploid cybrids, realizes the effective transfer of Wenzhou Satsuma mitochondrial genome, and provides a novel method and new germplasm materials for diploid Satsuma breeding.

Provided is a method for producing a rice F1 seed including crossing a rice male sterile line of Koshihikari containing one or more genes selected from the group consisting of the Pb1 gene derived from a rice (Oryza sativa L) cultivar Modan and the Cr1 gene derived from Oryza nivara, as a seed parent, with a rice fertility restorer line as a pollen parent, and collecting the first filial generation seed (F1 seed) from the post-crossing seed parent; a rice F1 seed which is obtained by the above-described method for producing a rice F1 seed; and a rice cytoplasmic male sterile line including one or more genes selected from the group consisting of the Pb1 gene derived from rice (Oryza sativa L) cultibar Modan, and the Cr1 gene derived from Oryza nivara.

The invention discloses a method for quickly cultivating a novel cytoplasm male sterility line of rice. The method comprises the following steps of: using dongxiang wide rice as a female parent and a variety or a strain which is good in column exposure, excellent in comprehensive character and good in combining ability as a male parent to hybridize to obtain F1; and then, performing backcrossing by using the varieties and strains as the backcrossing male parents; and from second backcrossing generate, selecting a single plant with pollen sterility phenotype similar to that of the backcrossing male parent to perform backcrossing with the backcrossing male parent, and continuously backcrossing for 5-6 times to obtain the male sterility line with dongxiang wide rice as an aborted cytoplasm source. The backcrossing male parent variety or strain is a maintainer line of the male sterility line. The novel cytoplasm male sterility line of rice can be cultivated in an accelerated manner by the technical method provided by the method disclosed by the invention, and the method is good in repeatability and reliable in result.

The invention provides a method for identifying soybean RN type cytoplasmic male sterile line seed purity through a molecular marker. The method comprises the steps of: extracting genomic DNA of an RN type cytoplasmic male sterile line seed; carrying out PCR (Polymerase Chain Reaction) amplification by taking the genomic DNA as a template and utilizing a primer InDel-cms1, and carrying out gel electrophoresis analysis on an amplification product, wherein the length of a sterile line fragment after amplification is 200bp, and the length of a maintainer line fragment after amplification is 212bp; and accurately distinguishing the sterile line and the maintainer line through the length difference of the fragments, wherein the seed with the amplified fragment different from the amplified sterile line fragment is a false sterile line seed, and the percentage of the number of sterile line fragments with specific length obtained by detection accounting for all the detected seeds is the purity of RN type cytoplasmic male sterile line seeds. The method can be used for rapidly, accurately and reliably identifying the maintainer line mixed in the RN type soybean cytoplasmic male sterile line by a molecular biology experiment means so as to ensure the purity of sterile line seeds.