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73 results about "Nucleoplasm" patented technology
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Similar to the cytoplasm of a cell, the nucleus contains nucleoplasm, also known as karyoplasm, or nucleus sap. The nucleoplasm is a type of protoplasm, and is enveloped by the nuclear envelope (also known as the nuclear membrane). The nucleoplasm includes the chromosomes and nucleolus. Many substances such as nucleotides (necessary for purposes such as DNA replication) and enzymes (which direct activities that take place in the nucleus) are dissolved in the nucleoplasm. The soluble, liquid portion of the nucleoplasm is called the nucleosol or nuclear hyaloplasm.
Method for agrobacterium tumefaciens-mediated genetic transformation of sugarcane
InactiveCN102154364AOvercome serious shortfalls in conversionAddress serious deficienciesPlant tissue cultureHorticulture methodsMetal ArtifactTransformation efficiency
The invention discloses a method for agrobacterium tumefaciens-mediated genetic transformation of sugarcane. The method comprises the steps: leading agrobacterium tumefaciens liquor carrying plant expression vectors to infect embryogenic callus of the sugarcane; and selecting and proliferating resistant calli to induce the resistant calli to obtain the resistant buds of the sugarcane, wherein matrix attachment region sequences are connected at two sides of a gene expression box of the plant expression vector. According to the method, MARs (metal artifact reduction) sequences are built at two sides of the gene expression box of the plant expression vector, thus greatly improving the exogenous gene transformation efficiency and seedling rate of the sugarcane, and being simple in process flow and low in cost.
Owner:广西作物遗传改良生物技术重点开放实验室
Reporter system for high throughput screening of compounds and uses thereof
ActiveUS20130005666A1High throughput screeningImprove throughputOrganic active ingredientsPeptide/protein ingredientsHigh-Throughput Screening MethodsNrf2 activation
The NF-E2-related factor 2 (Nrf2) is a key transcriptional regulator of antioxidant defense and detoxification. To directly monitor stabilization of Nrf2 we fused its Neh2 domain, responsible for the interaction with its nucleocytoplasmic regulator, Keap1, to firefly luciferase (Neh2-luciferase). It is shown herein that Neh2 domain is sufficient for recognition, ubiquitination and proteasomal degradation of Neh2-luciferase fusion protein. The novel Neh2-luc reporter system allows direct monitoring of the adaptive response to redox stress and classification of drugs based on the time-course of reporter activation. The novel reporter was used to screen a library of compounds to identify activators of Nrf2. The most robust and yet non toxic Nrf2 activators found—nordihydroguaiaretic acid, fisetin, and gedunin-induced astrocyte-dependent neuroprotection from oxidative stress via an Nrf2-dependent mechanism.
Owner:CORNELL UNIVERSITY
Method for imaging live nucleus and cytoplasm and application thereof in monitoring live nucleus and cytoplasm signal pathway
InactiveCN101738462AMaintain a functional environmentEliminate phototoxicityBiological testingFluorescence/phosphorescenceAbnormal tissue growthMicroscopic image
This invention discloses a method for imaging live nucleus and cytoplasm, which comprises the following steps that: (a) live cells are irradiated by a near-infrared femtosecond laser which is focused by a microscope; (b) the live cells produce two-photon fluorescence and second harmonic under the action of the near-infrared femtosecond laser focused by the microscope; and (c) the two-photon fluorescence and the second harmonic are subjected to microscopic imaging at the same time, the two-photon fluorescence performs microscopic imaging on target molecules which are marked by fluorescence, and the second harmonic performs microscopic imaging on the nucleus. The invention also discloses application of the method for imaging the live nucleus and cytoplasm in monitoring a live nucleus and cytoplasm signal pathway, eliminates photoinduced toxicity and photobleaching, and can observe the live cells for a long time without affecting the activity of the cells and furthest maintain the functional environment of the cells. The method can be applied to the level layer research of cell molecules in a tumor generation mechanism and the research of a pharmacodynamical mechanism of traditional Chinese medicinal herbs.
Owner:SOUTH CHINA NORMAL UNIVERSITY
Whole breast tumor small-interval subserial pathological slice preparation method
ActiveCN109000956AAchieve preliminary cuttingAvoid bending deformationWithdrawing sample devicesPreparing sample for investigationAbnormal tissue growthParaffin wax
The invention discloses a whole breast subserial large slice preparation method. The method comprises the following steps of collecting a tumor sample; performing continuous parallel splitting to obtain sample blocks which are split in parallel; performing soaking and fixing in a neutral formaldehyde solution with the concentration of 4% for 24 hours; removing redundant fat tissues by clipping; performing continuous parallel cutting in parallel to an original slice again to form a slice shape; performing continuous fixing for 48 hours by using the neutral formaldehyde solution with the concentration of 4%; performing sequential dehydration by adopting gradient ethanol; performing transparency treatment by using xylene; performing wax dipping in an electric heating constant temperature incubator at 62-65 DEG C to obtain a paraffin pathological sample; and slicing the paraffin pathological sample by adopting a rotary paraffin slicing machine, wherein each sample block can be subjected toconventional pathological staining including HE through continuous slicing. The thickness of a large slice prepared with the method can reach the conventional slice thickness of 4-5 microns; the slice is complete in tissue structure, smooth, clean and free of folds and pollution; no slice stripping phenomenon occurs; the cell nucleoplasm proportion is clear; and a staining result is consistent with a conventional clinical staining result.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV
Breeding method of cabbage type rape woad oil cytoplasm male sterile line
InactiveCN105230486AStable sterility traitsExpansion of sterile cytoplasmic resourcesPlant tissue cultureHorticulture methodsProtoplastSomatic cell
The invention belongs to the technical field of rape molecular breeding, and specifically relates to a breeding method of cabbage type rape woad oil cytoplasm male sterile line. The invention also relates to a protoplast fusion technology to recombinate the cytoplasm and nuclear genome of cabbage type rape-woad so as to establish cytoplasmic male sterility of recombinant cytoplasm. The breeding process comprises the following steps: obtaining the leaf meat protoplast of cabbage type rape-woad through separation and extraction, obtaining the protoplast fused somatic cell hybrid of cabbage type rape-woad through a cell fusion method; taking the obtained somatic cell hybrid of cabbage type rape-woad and leaf meat protoplast cell hybrid of cabbage type rape-woad as the female parent, taking cabbage type rape as the male parent, carrying out multi-generation backcross, and obtaining cytoplasmic male sterile line with carpelloid stamen; wherein the mitochondrion gene PCR amplification verifies that mitochondrial genome carries out recombination during the fusion process, thus the nucleoplasm becomes uneven, and the sterile character appears.
Owner:HUAZHONG AGRI UNIV
Method for quickly extracting DNA from plant leaves
The invention discloses a method for quickly extracting DNA from plant leaves, and relates to a method for quickly extracting beet total DNA by using leaf tissues. The method comprises the following steps: directly grinding leaves in a grinding pestle centrifuge tube, performing high-temperature water bath treatment by using an extracting buffer solution, and adding a separation medium to perform high-speed centrifugation. The method disclosed by the invention is simple in step, less in required tissue sample, less in toxic chemical reagent, low in cost, short in operation time and good in experimental effect. The obtained DNA can be directly used for PCR (polymerase chain reaction) amplification of nucleoplasm gene fragments, and applied to molecular biology tests of molecular markers, fingerprint spectrums and the like, so that a DNA nucleic acid base material is provided for agricultural biotechnology molecular breeding and researches in the field of molecular biology.
Owner:HARBIN INST OF TECH
Method for transforming hot-pepper nucleo-cytoplasmic interactive male-sterile line, homo-maintainer line and homo-restorer line
InactiveCN101971774ASave labor costsReduce manufacturing costPlant genotype modificationHot peppersEconomic benefits
Owner:GUIZHOU HORTICULTURAL INST (GUIZHOU HORTICULTURAL ENG TECH RES CENT)
Cell sorting method for affine propagation clustering
ActiveCN101853507AEffective classificationImprove real-time performanceImage analysisCharacter and pattern recognitionAlgorithmDiagonal
The invention relates to a cell sorting method for an affine propagation clustering, which comprises the following steps: (1) selecting a circularity parameter C and a rectangularity parameter R of a cell image, designing a sample coordinate X sample=lambda C+(1-lambda) R, selecting an area parameter Area of the cell image as another sample coordinate Y sample and selecting a nuclear-cytoplasmic ratio parameter prop of the cell image as another sample coordinate Z sample, wherein lambda represents a prior input value; (2) using euclidean distance of the three-dimensional sample coordinates as sample distance, wherein a diagonal value of an S matrix of the affine propagation clustering is an average value of distances among the samples; (3) under the initial condition, setting an membership grade matrix A(i, k)=0, updating a matrix R and updating a matrix A; and (4) stopping after the number of iteration times is set and obtaining different types of cells from a sorting result. The invention provides the cell sorting method for the affine propagation clustering, which is suitable to process mass data, has excellent real-time property and can effectively carry out cell sorting.
Owner:山东兰香食品有限公司第一分公司
Breeding method for chilli pepper nuclear male sterile dual purpose line and nuclear substance male sterile recovery line
ActiveCN1820581ASolve the problem of few recovery sourcesHigh purityPlant genotype modificationAgricultural scienceHot peppers
The present invention relates to plant breeding technology, and is especially anther cultivation technological method of breeding unclear sterile dual purpose line and unclear substance sterile recovery line of capsicum. Extracorporeal anther cultivation technology is used in selective breeding of unclear sterile dual purpose line and unclear substance sterile recovery line of capsicum. That is, the dual purpose line is obtained through test cross of the fertile plant and sterile plant in contemporary anther cultivation offspring of dual purpose line hybrid, F1 selfing, test cross of similar fertile plant and sterile plant in the offspring lines and continuous two generation selection; and the recovery line is obtained through the test cross of the fertile plant and sterile plant in the contemporary anther cultivation offspring of three line hybrid with available sterile line, selectively breeding the F1 with fertility recovering rate of 100 % and stable fertility to obtain fertile plant as the corresponding recovering plant and final selfing.
Owner:北京市海淀区植物组织培养技术实验室
Breeding method of polima genic-cytoplasmic male sterile line
InactiveCN102835303AImprove breeding efficiencyBroaden the Restoration SpectrumWater resource protectionPlant genotype modificationBiologyCytoplasmic male sterility
The invention discloses a breeding method of a polima genic-cytoplasmic male sterile line. The method mainly comprises the steps of obtaining a cytoplasmic male sterile plant by hybridizing, backcrossing, multiple crossing and an anther culture method; performing hybridizing, multi-generation backcrossing or multi-generation sister-crossing with strains with restoring gene, preparing a target sterile line and a target maintainer line; taking the target maintainer line as a recurrent parent, performing hybridizing and backcrossing with strains with fertile cytoplasm; replacing the sterile cytoplasm of the target maintainer line with fertile cytoplasm; after selfing of the cytoplasm-replaced target maintainer, performing test-crossing with the target sterile line; and identifying sterile maintaining ability of the maintainer plant and breeding the sterile line. The method can effectively remove minor restoring gene, improve outcrossing ability of the sterile line, improve breeding efficiency of the male sterile line, obtain excellent sterile line, breed full maintenance line from the sterile cytoplasmic resource which is difficult to abort thoroughly, expand utilization range of the polima genic-cytoplasmic male sterile resource and enlarge restoring spectrum of the male sterile line.
Owner:HUNAN HYBRID RICE RES CENT +1
Method and use of selective culture of male sterility line of rice
InactiveCN1489895AUniform agronomic traitsSpeed up and expandPlant genotype modificationDiseaseZoology
A selective culture method for the male sterility line of paddy rice features that the sterile cytoplasm of nucleoplasmic interaction type male sterility line of paddy rice is used to realize the hybridization between maintenance line and other excellent variethes, and the cultured said nucleoplasmic interaction type male sterility line as female parent and the male sterility recovery line as male parent are hybridized to obtain new combination. The resultant new combination has the advantages of high adaptability, stress resistance and disease and past resistance, and improved quality and yield of rice.
Owner:RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
Method for reproducing and production of hybrid seeds of rice karyoplasmic interaction type incomplete male sterility line
InactiveCN101044833AImprove efficiencyIncrease outcrossing ratePlant genotype modificationRice cultivationHybrid seedInteraction type
A reproductive and seed culturing method for the karyoplasmic interaction type incomplete male sterility line of rice includes such steps as choosing male parent and female parent (Karyoplasmic interaction type incomplete male sterility line), sowing, irrigating with cold water when the ambient temp is higher than the critical temp of fertility transform, hybridizing, growing and harvesting.
Owner:HUNAN HYBRID RICE RES CENT +1
Method for improving compatibility and development rate of interracial nuclear transfer embryo nucleoplasm
InactiveCN102382857AGood compatibilityIncrease development rateEmbryonic cellsGenetic engineeringEmbryoPerivitelline space
The invention provides a method for improving compatibility and development rate of interracial nuclear transfer embryo nucleoplasm. The method includes the steps: (a) a cell nucleus of a nuclear donor cell is moved into a perivitelline space of an enucleated oocyte of a non-human mammal and integrated by means of electric stimulation, so that a reconstructed ovum is formed, (b) a human oocyte is moved into the reconstructed ovum in the step (a), and (c) the reconstructed ovum in the step (b) is electrically stimulated so as to form a reconstructed ovum composed of the cell nucleus of a human cell, the enucleated oocyte of the non-human mammal and the human oocyte. The embryo development rate can be increased remarkably by the method.
Owner:SHANGHAI TRANSGENIC RES CENT
Method for transplanting consubstantial cell nucleus
The present invention belongs to the field of bioengineering technology, and is especially biological consubstantial cell nucleus grafting and cloning technology. By means of biological cloning technology, the present invention takes consubstantial nucleus donor cell and denucleated egg mother cell with clear genetic background for consubstantial cell nucleus grafting to prepare cloned animal. The present invention has relatively high matching between the donor nucleus DNA and acceptor cytoplasm and the electronic compatibility inside nuclear substance, and can improve the development of cloned embryo and raise cloning efficiency. The said technology may be used in cloning animal to obtain high quality livestock.
Owner:SHANGHAI INST OF MEDICAL GENETICS SHANGHAI CHILDRENS HOSPITAL
Kit for building a model of a cell
A method of creating a model of a biological cell. The method features providing a circular base with indentations disposed therein, forming a cell membrane and at least one organelle from a mixture formed by mixing all-purpose flour and water. Organelles may include a nuclear membrane, a cell wall, mitochondria, a smooth endoplasmic reticulum (ER), a rough ER, a vacuole, a golgi apparatus, a nucleolus, a nucleoplasm, chromatin, a ribosome, DNA, RNA, transcription machinery, a histone, and a microtubule. The outside edge of the base is lined with the cell membrane. The organelles are inserted into indentations or atop the base. The organelles can be secured with glue.
Owner:POMPEY AUDREY SIOUX
Method for combining hybrid first generation seed with cytoplasmic male sterility system
The invention relates to a method for combining the first generation of hybrid seeds by kenaf cytoplasmic male sterile line, and belongs to the technical field of combination of crop hybrid seeds. The method is characterized in that the kenaf cytoplasmic male sterile line (also called cytoplasmic-nuclear male sterile line) is taken as female parent, and is combined and crossbred with any one of male fertile varieties (lines), and the male fertile variety (line) is in accordance with the breeding target and is taken as male parent, without considering whether the first generation of hybrid restores the fertility. The method can greatly improve the freedom degree of kenaf hybridized combination, and is easy to breed the hybridized combination with comparative advantages.
Owner:GUANGXI UNIV
Method for carrying out breeding crossbred crop by using male sterile line in type of nucleo-cytoplasmic interaction
InactiveCN101049082AEasy to controlFavorable another leap forwardPlant genotype modificationNucleoplasmGene
A method for preparing the seeds of hybrid crops by using the minor restoring gene of nuclear-cytoplasm interactive male sterility line includes such steps as choosing the nuclear-cytoplasm interactive male sterility line A, its maintenance line B and its recovery line R, preparing the subisopolar gene line B' or B' of said B and with the minor restoring gene, discriminating the fully male sterility A / B' F1 or A / B' / / B' F1, and using A / B' / / R or A / B' / / B' / / / R to produce the seeds of hybrid crops.
Owner:HUNAN HYBRID RICE RES CENT +1
Method for culture of new sterile line of rice
InactiveCN1401221APromote safe productionComplete male sterilityPlant genotype modificationBiotechnologyGene
A method for culturing a novel sterile line of paddy rice is characterized by that the cytoplasm of male sterility line of non-flutinous rice is used as the doner of the cytoplasm for novel sterile line and the photosensitive karyosterile line of non-glutinous rice containing male sterile maintainer gene is used as the maintainer line of novel sterile line. Its advantage is full male sterility in either longer or shorter day condition.
Owner:RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
Method for breeding onion male sterile line and maintainer line by utilizing molecular markers
ActiveCN103981281AMark stableEasy to operateMicrobiological testing/measurementDNA/RNA fragmentationAgricultural scienceGenotype
The invention discloses a method for breeding onion male sterile line and maintainer line by utilizing molecular markers. A PCR (polymerase chain reaction) experiment is performed to detect single onion ball nucleoplasm genotype by utilizing specific primers (disclosed as SEQ ID NO.1-6) to screen a sterile strain and a maintainer strain onion ball, thereby implementing two-line matching. A plurality of sets of primers are designed according to Ms allele flanking sequences (F1890 and S1887) and onion cytoplasmic male sterility specific sequence mt472; and scientific experiments are performed to develop a molecular marker detection system and detection kit based on multiplex PCR. The detection system can distinguish 6 karyoplasm fertility types of onions through one PCR experiment; and the detection system is utilized to directly breed the sterile line and maintainer line from the open pollination colony, thereby implementing one-step matching of two lines. The development of the multiplex PCR detection system has important meanings in accurate and quick identification of onion nucleo-cytoplasmic interreaction male sterility karyoplasm genotype.
Owner:VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
Method for combining hybrid bibasic seed with cytoplasmic male sterility system
InactiveCN101401541BYield is not adversely affectedYield impactPlant genotype modificationHybrid seedPollination
Owner:GUANGXI UNIV
Method for combining hybrid bibasic seed with cytoplasmic male sterility system
InactiveCN101401541AYield is not adversely affectedYield impactPlant genotype modificationHybrid seedPollination
The invention relates to a method for combining the first generation of hybrid seeds by kenaf cytoplasmic male sterile line, and belongs to the technical field of combination of crop hybrid seeds. The method is characterized in that the cytoplasmic male sterile line (also called cytoplasmic-nuclear male sterile line) taken as female parent and restoring line taken as male parent are combined into the first generation of the hybrid seeds which breed the second generation of the hybrid seeds through inbred breeding. The method is novel due to that the separation of male sterile plants and fertile plants exists in the second generation of the hybrid seeds, but kenaf takes the harvest of stalks or bast fibre as breeding aim and does not need seeds, so the male sterile plants have no adverse affection to the yield of the bast fibre. The method saves the labor input of kenaf chemical hybridizing and artificial pollination, and can guarantee the purity of the hybrid seeds because of stable fertility of the kenaf cytoplasmic male sterile line.
Owner:GUANGXI UNIV
Culture method of dental pulp mesenchymal stem cells
InactiveCN108277202AClosely arrangedIll-definedCell dissociation methodsSkeletal/connective tissue cells3D cell cultureHeart disease
The invention discloses a culture method of dental pulp mesenchymal stem cells. The culture method comprises the following steps: preparing a dental pulp sample, separating and culturing the dental pulp mesenchymal stem cells, carrying out colonized culture on separated dental pulp mesenchymal stem cells, detecting the dental pulp mesenchymal stem cells, and detecting and staining the dental pulpmesenchymal stem cells. The culture method of the dental pulp mesenchymal stem cells disclosed by the invention has the beneficial effects that 1, the cells cultured by being cloned and separated areclosely arranged, so that central cells are unclear in boundaries, round or irregular and cell nodules are formed, peripheral cells are polygonal or short fusiform, less in cytoplasm, large in proportion of nucleoplasm and obvious in nucleoli and some cells are fibrillar; 2, the problem of serious immunological rejection is not generated; 3, the dental pulp mesenchymal stem cells are convenient intaking materials and safe and small in cross-infection risk, thereby being capable of being used for repairing defective teeth and tooth regeneration; and 4, the dental pulp mesenchymal stem cells can promote skin wounds to heal and regenerate, delay aging or cure blindness, and further can be used for treating treat heart diseases, rheumatoid arthritis, burns, stroke or cartilage damage and thelike and is wide in use.
Owner:安莱(西安)健康产业有限公司
Method suitable for in-situ hybridization of cherax quadricariratus gonadal tissue mRNA paraffin section
ActiveCN109988846ARealize the display effectClear specificityMicrobiological testing/measurementClimate change adaptationBiologyNucleoplasm
The invention provides a method suitable for in-situ hybridization of a cherax quadricariratus gonadal tissue mRNA paraffin section, and belongs to the technical field of in-situ hybridization. The method comprises the steps of gonadal tissue embedding, paraffin sectioning, in-situ hybridization, and photographing and recording. The sequence of a probe used in in-situ hybridization is as shown inSEQ ID NO.3. The probe has clear specificity on tissue positioning of a dsx gene, so that the display effect on the mRNA level of the dsx gene is realized. The method for mRNA in-situ hybridization ofthe paraffin section is established in cherax quadricariratus to research expression modes of related genes in the gonad, and the expression location of the sex-related genes of the cherax quadricariratus in the gonad can be clearly described. The method avoids tissue shrinkage and cell deformation while the dewaxing effect of the paraffin section is good, so that the tissue is still kept in theoriginal position, the tissue morphology is kept complete, the enzymolysis effect can be improved, cell coloring is good, nucleoplasm is clear, and the in-situ hybridization accuracy is finally improved.
Owner:ZHEJIANG INST OF FRESH WATER FISHERIES
Temperature-sensitive rice male sterility line breeding method with Honglian cytoplasm
InactiveCN1817098AIncrease useImprove efficiencyVector-based foreign material introductionPlant genotype modificationBiotechnologyGenome
A method for selectively culturing the light and temp sensitive male karyosterile line of rice with the red lotus type cytoplasm features the continuous backcrosses between the red lotus type sterile line as female parent and the light and temp sensitive karyosterile line carrying the red lotus type sterile line's restoring gene. If said light and temp sensitive karyosterile line does not carry the red totus type sterile line's restoring gene, said continuous backcross must be performed after the red lotus type cytoplasm restoring gene is introduced to it by hybridization or molecular marking.
Owner:HUBEI UNIV
Reporter system for high throughput screening of compounds and uses thereof
ActiveUS9200046B2Improve throughputOrganic active ingredientsPeptide/protein ingredientsNrf2 activationHigh-Throughput Screening Methods
Owner:CORNELL UNIVERSITY
Method for induced breeding of cytoplasmic-nuclear male-sterile line by using core haploidy
InactiveCN104823840AImprove efficiencyImprove the purity of seed productionPlant genotype modificationHybrid seedGermplasm
The invention relates to the field of corn hybridization, in particular to a method for induced breeding of a cytoplasmic-nuclear male-sterile line by using core haploid. The method comprises the following steps of performing hybridization by using cytoplasmic male sterility cytogene S, gene (ig) of which cytogene contains indeterminate gametophyte and an inducing line of a selection marker gene as a female parent and using an inbred line or double haploid or an excellent low-generation material of which the cytogene is N and the cell nucleus fertility recovery gene is rfrf as a male parent; screening to obtain progeny of haploid of which the cytogene is S and the cell nucleus fertility recovery gene is rf; and performing backcross on the progeny and the male parent to obtain the cytoplasmic-nuclear male-sterile line of a selfing line or double haploid of which the cytogene is S and the cell nucleus fertility recovery gene is rfrf. By the method, the improvement efficiency of the cytoplasmic-nuclear male-sterile line is improved obviously, an excellent germplasm can be quickly applied to sterilization production of hybrid seeds, purity of production of the hybrid seeds can be greatly improved, and the labor cost is saved.
Owner:JILIN ACAD OF AGRI SCI
Ovine oocyte in-vitro double-phase maturation method, pre-incubation liquid for ovine oocyte in-vitro double-phase maturation and kit
ActiveCN108118027AImprove in vitro development abilityPromote healthy developmentCulture processCell culture active agentsBiotechnologyPyrrolidinones
The invention discloses an ovine oocyte in-vitro double-phase maturation method, pre-incubation liquid for ovine oocyte in-vitro double-phase maturation and a kit and belongs to the field of embryo engineering. The pre-incubation liquid is prepared from an oocyte in-vitro basic culture medium TCM199 added with C-type natriuretic peptide and polyvinylpyrrolidone (PVP). Therefore, the C-type natriuretic peptide provided by the invention is pre-incubated and is combined with a double-phase culture system of in-vitro maturation, so that nucleoplasm synchronous maturation of an ovine oocyte can beeffectively facilitated and the in-vitro development capability of the oocyte is improved; high-quality ovum sources and embryos can be provided for ovine in-vitro embryo production; requirements on scientific researches and production are met better.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY
Method for rapidly breeding nucleo-cytoplasmic interaction sterile lines
The invention belongs to the technical field of agricultural seed breeding and discloses a method for rapidly breeding nucleo-cytoplasmic interaction sterile lines. The method comprises the following steps of: utilizing the existing male sterile lines and a plurality of homozygous and excellent selfing line male parent materials to carry out paired testcross; carrying out simultaneous selfing on male parents, and analyzing offspring fertility; selecting 12 sterile plants in a fertility-separation testcross combination for one-to-one backcross with male-parent selfing offspring; and rapidly transbreeding out male sterile lines and maintainer lines in a third-generation backcross combination; and the method comprises the detailed steps of: screening a first generation, screening a second generation and screening a third generation. The method disclosed by the invention has the advantages that excellent selfing lines can be rapidly transbreeded into the male sterile lines, the maintainer lines are screened out, and the sterile degree reaches 100%; 12 plants are selected in the male-parent selfing offspring to carry out testcross with 12 sterile plants in F1 generation for three times repeatedly, so that the probability of obtaining the sterile lines and the maintainer lines is increased.
Owner:GUIZHOU SERICULTURE RES INST GUIZHOU PEPPER RES INST
Method for acquiring heterogeneity interspecies-cloned yak premium embryos
InactiveCN103409467AIncrease formation rateIncrease cell numberFermentationGenetic engineeringAgricultural scienceGermplasm
The invention discloses a preparation and in-vitro culture method of interspecies-cloned yak embryos. According to the method, yak-cattle interspecies-cloned embryos are constructed by using yak mammary glandular cells as donor cells and cattle or milch cow enucleated oocytes as receptor cytoplasts by a somatic nucleus transplantation technique; and an electrical activation-chemical activation-reelectrical activation mode and a self-made sequential culture system are utilized to acquire the high-efficiency high-activity yak-cattle interspecies-cloned embryos. The method disclosed by the invention can increase the yak breeding speed, shorten the yak breeding cycle, lower the yak production cost, enhance the breeding efficiency, reduce the damage level of the grassland to some extent, and effectively protect the species resource of the yak. Besides, the invention has important meanings for promoting research of interspecies-cloned yak, transgenic cloned yak embryos and nucleo-cytoplasmic interaction.
Owner:SOUTHWEST UNIVERSITY FOR NATIONALITIES
Cancer associated gene mina 53, protein mina 53 and monoclonal antibody thereof
InactiveUS20060234318A1Prevent proliferationAnimal cellsImmunoglobulins against cell receptors/antigens/surface-determinantsTranscription initiation siteFhit gene
Myc protein is an unevenly distributed intermediate agent for cell proliferation, and activates a gene expression via E.box. Mina 53 gene encodes a protein of 53 kDa molecular weight and is present in the nucleoplasm and nucleolus. Mina 53 mRNA and protein expression are induced by artificial introduction of c-Myc activity. E.box site is present in the vicinity of the transcription initiation site of mina 53 gene, and the expression from mina 53 promoter is activated by the c-Myc through the medium of E.box. Specific inhibition of the mina 53 expression in HeLa cells and rat fibroblast cells 3Y1 having high expression c.myc strikingly inhibited the cell proliferation. Combination of these results shows that the mina 53 is a Myc target gene and is associated with the cell proliferation of mammal.
Owner:GAKKOUHOUJIN KURUME UNIV
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