15 results about "Nucleoplasm" patented technology

The invention discloses a gray clustering characteristic distribution-based cell nucleoplasm segmentation method, which comprises the following steps of: 1) constructing a self-supervised network, and solving a cell intensity distribution diagram of a cell image; 2) determining cell candidate regions in the intensity maps of the cell distribution, the staining intensities of the intensity maps of the cell distribution are different, but the deepest part in the image must belong to a cell nucleoplasm region, so that the candidate regions of the cell nucleoplasm are solved by adopting an iterative search mode; and 3) carrying out accurate segmentation on the obtained candidate cell region to obtain a segmentation result of the cell nucleoplasm, and finally, correspondingly drawing a minimum circumscribed contour of the cell nucleoplasm in the original color map. The invention belongs to the technical field of image processing and biomedicine, and solves the problems that in the prior art, when a cell image is processed, the extraction part is inaccurate, impurities are easy to mix, and the detection precision is influenced.

The invention belongs to the technical field of nucleic acid extraction, and particularly relates to a method for separating and extracting purified nucleic acid from different cell components. The invention develops a separation and purification method suitable for liquid-phase RNA (Ribonucleic Acid) of a cytoplasm component, a nuclear free component and the like, so that the extracted RNA can be compatible with the highest standard of nucleic acid mass spectrum and high-throughput sequencing, and the operation flow is simpler, the time is shorter, the cost is lower, and the extraction effect is better and more efficient; meanwhile, a corresponding nucleoplasm DNA / RNA separation buffer is developed on the basis, and an efficient nucleic acid purification process is matched, so that the genome DNA can be completely extracted with high quality and high purity while high-quality chromatin RNA is obtained, and another level of information, namely genome DNA information, is provided for a classical component separation scheme of RNA dynamic biology; therefore, all nucleic acid information of each component of the cell can be efficiently separated and purified.

The invention belongs to the cross technical field of cell engineering, nano-drugs and biological manufacturing, and particularly discloses a drug-loaded vesicle based on denucleated cells as well as a preparation method and application of the drug-loaded vesicle. According to the invention, a cell suspension and a cytoskeleton relaxant are co-incubated to relax an actin skeleton and weaken nucleoplasm connection; then loading the treated cell suspension on a multi-layer discontinuous density gradient centrifugal medium, realizing physical separation of cell nucleuses and cytoplasm through high-speed centrifugation according to buoyancy density difference, and collecting components of a specific interface to obtain high-purity denucleated cells with a complete membrane structure; then co-incubating the denucleated cells and ROS response type lipidosome (co-carrying therapeutic siRNA and a sound-sensitive agent Ce6) prepared in advance, so that the lipidosome is wrapped or anchored by a denucleated cell membrane; finally, the composite system is subjected to extrusion treatment through a microporous membrane, the drug-loaded vesicles uniform in particle size and stable in structure are obtained, and the drug-loaded vesicles are suitable for various application scenes such as anti-tumor treatment and RNA vaccine delivery.

The invention discloses application of carfilzomib in preparation of a medicine for preventing and treating Nipah virus, and belongs to the technical field of medicine. The method mainly aims at ubiquitination modification of nipah virus M protein, the carfilzomib is used for inhibiting a host proteasome path, free ubiquitination factors in cells are depleted to achieve the effect of inhibiting ubiquitination of the M protein, then nucleoplasm shuttle of the M protein is inhibited, replication of the nipah virus is inhibited, and the effect is good. At present, most of research and development of virus medicines and vaccines are designed or modified aiming at viruses, a new thought is provided for research and development of medicines and vaccines in the future, and a new application is also provided for Carfilzomib.

This disclosure provides methods and compositions to increase the efficiency of targeted coding RNA pseudouridylation by H / ACA snoRNA, by localizing the H / ACA snoRNA more to the nucleoplasm where pre-mRNAs are transcribed and processed into mature mRNAs versus the nucleolus, where H / ACA snoRNAs natively modify ribosomal RNAs.

The invention provides a method for screening and verifying circMKLN1 circular RNA (Ribonucleic Acid) capable of regulating and controlling triple negative breast cancer, circMKLN1 is formed by transcription and reverse splicing cyclization of 4-7 exons of an MKLN1 gene, and the length of circMKLN1 is 535bp. The primer of the circMKLN1 is used for carrying out PCR (Polymerase Chain Reaction) amplification and then is sent to a living organism company for Sanger sequencing, and the result shows that the specific splicing site of the circMKLN1 is found. The cDNA and the gDNA are used as templates, PCR amplification is carried out by using a circMKLN1 convergence primer and a circMKLN1 divergent primer, and the result shows that the circMKLN1 divergent primer can only be amplified in the cDNA and is not amplified in the gDNA, so that the existence of the circMKLN1 is further proved. The RNase R and actinomycin D experiments show that the circMKLN1 has higher resistance to the RNase R, and the half-life period of the circMKLN1 is far longer than the half-life period of linear MKLN1 mRNA (messenger ribonucleic acid). A nucleoplasm separation experiment shows that circMKLN1 mainly exists in cytoplasm.

The invention belongs to the field of cell culture, and particularly discloses application of dominant follicle exosomes in oocyte in-vitro maturation culture, after the dominant follicle exosomes with the concentration of 10 ng / mL-200 ng / mL are added into a porcine oocyte in-vitro maturation culture solution for porcine oocyte in-vitro maturation culture, the first polar body discharge rate is remarkably increased, and the first polar body discharge rate is remarkably increased. Therefore, the dominant follicle exosome can effectively improve the in-vitro maturity of the porcine oocytes; the dominant follicle exosomes with different concentrations are added, so that the diffusion area of cumulus cells is remarkably increased; after the dominant follicle exosome is added, the ROS fluorescence intensity is obviously reduced, and the GSH fluorescence intensity is obviously increased; by adding the dominant exosome, the remodeling of the mitochondrial structure and function in the oocyte is accelerated; adding of the dominant exosome promotes functional remodeling of the mitochondrial complex I to increase ATP generation and reduce oxidative stress so as to promote nucleoplasm co-maturation of oocytes. The subsequent embryonic development can be promoted.

The invention relates to the technical field of medicines, and discloses an application of a tetra-substituted pyrrole derivative in preparation of a product for treating neurodegenerative diseases. According to the tetra-substituted pyrrole derivative or the pharmaceutically acceptable application of the tetra-substituted pyrrole derivative in preparation of products for treating neurodegenerative diseases, the tetra-substituted pyrrole derivative is high in cell safety, and the neuroprotective effect on glutamic acid injury is far better than that of atrasentan and clinical standard drugs edaravone and riluzole; not only can the nucleoplasm dislocation of the TDP-43 be effectively corrected, but also the capability of removing insoluble pathological aggregates, which is not possessed by the atrasentan, is achieved; the co-localization of the TDP-43 and the stress particle protein G3BP1 can be more effectively inhibited, and the formation of pathological particles is intervened from the source; on the whole animal model, the tetra-substituted pyrrole derivative successfully converts the advantage of the cellular level into remarkable survival benefit, and the life of the disease model fruit flies is remarkably prolonged.

The invention relates to the technical field of biology, in particular to an anti-root-knot nematode method and system for regulating and expressing hpRNA by targeting a plant root white body and application of the anti-root-knot nematode method and system. According to the invention, it is found for the first time that root-knot nematode can uptake a plastid endogenous nucleic acid RNA molecule rbcL and dsRNA of an expressed exogenous non-nematode target, so that a vector for starting hpPAS4 expression by a T7 promoter is constructed, and plant plastid transformation is mediated by a gene gun, so that a plastid-transformed transgenic plant is obtained. Besides, T-DNA insertion is mediated by agrobacterium tumefaciens, a root-specific promoter TobRB7 and a transport peptide RecA1 plastid are subjected to localization expression of T7RNA polymerase, and the T7RNA polymerase is integrated into a plant nuclear genome, so that nucleoplasm co-transformation is realized, and the plastid of the plant can efficiently express hpPAS4 by means of a T7 system. By utilizing the system, the expression of RNAi interference molecules is realized in the plastid (especially the white body of the root), the insufficient cell nucleus transformation is made up, a new way is opened up for the prevention and control of the root-knot nematode, and the system has great prevention and control value and application potential.

The invention belongs to the technical field of biological medicines, and particularly relates to discovery and application of an anti-cancer signal channel ALKBH5-LRPPRC-elF4E-XPO1. According to the invention, it is found for the first time that LRPPRC and ALKBH5 have interaction in a cell nucleus, and the interaction depends on m6A modification of RNA. Meanwhile, a brand new signal channel, namely ALKBH5-LRPPRC-elF4E-XPO1, is successfully disclosed by further exploring the physiological functions of the ALKBH5-LRPPRC-elF4E-XPO1. In addition, a complex formed by the interaction is also separated out, and the complex is used as a structural basis of RNAm6A demethylation nuclear export, so that RNA epigenetics and a specific nuclear export mechanism are organically combined in a breakthrough manner. In addition, experimental concept verification is carried out aiming at whether the new signal channel can regulate the expression of the proto-oncogene or not, and a new way and possibility are provided for accurately intervening elF4E nucleoplasm shuttle regulation.

The invention relates to a method for detecting nucleoplasm gene loci having an interaction effect with agronomic traits, which comprises the following steps: pairing nuclear genome SNP data and mitochondrial genome SNP data, calculating a genetic linkage imbalance coefficient between the nuclear genome SNP data and the nucleoplasm of each mitochondrial genome SNP, taking each nuclear genome SNP as a core, and calculating the genetic linkage imbalance coefficient between the nuclear genome SNP data and each mitochondrial genome SNP; nuclear genome SNP and mitochondrial genome SNP pairs with abnormal and prominent linkage strength are screened out to serve as candidate cytoplasmic interaction coupling pairs, a mixed linear model is constructed to implement GWAS analysis, and cytoplasmic gene loci with an interaction effect with agronomic traits are screened out. According to the method, the nucleoplasm gene loci with high relevance with the agronomic traits to be detected are screened out, so that the calculation power and time required by analysis can be greatly reduced, and the workload of molecular genetic research on the agronomic traits of the crops is reduced. According to the method, the result is obtained through analysis by software, the analysis steps are simple, the consumed time is short, the nucleoplasm gene loci are accurately detected, and the application range is wide.

The invention belongs to the technical field of biological medicine, and particularly relates to application of exosome miRNA as a gastric cancer diagnosis marker and a treatment target. The regulation and control effects of circulating exosome miRNA in early gastric cancer diagnosis and malignant progression are systematically analyzed, and it is found that miR-412-5p is remarkably low in expression in early gastric cancer. The scheme of the invention further confirms that the nucleoplasm transporter gene XPO1 is a direct target gene of miR-412-5p, and activation of a PI3K-AKT pathway can be driven by expression imbalance of the nucleoplasm transporter gene XPO1.

The application discloses a solution for separating fat cell nucleus and cytoplasm, and the solution comprises solution 1 and solution 2; wherein the solution 1 comprises 8-12 mM hydrochloride buffer, 0.03-0.15% Tween-20, 8-12 mM sodium chloride, 2-4 mM magnesium chloride, 0.8-2% bovine serum albumin and 0.4-0.6% ethyl phenyl polyethylene glycol; the solution 2 comprises 8-12 mM hydrochloride buffer, 8-25 mM sodium chloride, 1-15 mM magnesium chloride and 0.8-2.5% bovine serum albumin; the percentage is volume percentage. The application can realize the research on all cell types of fat tissues, greatly reduces the required amount of fat tissues and improves the possibility of carrying out single cell research. The application can be used for both transcriptome research and epigenetic research.

The invention discloses application of ixazomib in preparation of a medicine for preventing and treating Nipah virus, and belongs to the technical field of medicine. The method mainly aims at ubiquitination modification of the nipah virus M protein, the host proteasome pathway is inhibited through the ixazomib, free ubiquitination factors in cells are depleted to achieve the effect of inhibiting ubiquitination of the M protein, then nucleoplasm shuttle of the M protein is inhibited, replication of the nipah virus is inhibited, and a good effect is achieved. The ixazomib is used as a nipah virus inhibitor, belongs to a new application of an old medicine, and provides a new application for the ixazomib. And the safety of the ixazomib has no problem, so that the ixazomib can be applied clinically. In addition, at present, most of research and development of virus drugs and vaccine research are designed or modified aiming at viruses, and a new thought is provided for research and development of drugs and vaccines in the future.

This disclosure provides methods and compositions to increase the efficiency of targeted coding RNA pseudouridylation by H / ACA snoRNA, by localizing the H / ACA snoRNA more to the nucleoplasm where pre-mRNAs are transcribed and processed into mature mRNAs versus the nucleolus, where H / ACA snoRNAs natively modify ribosomal RNAs.