The present invention provides a method for rapidly determining the
open pollination intensity of macadamia nuts and its application, belonging to the field of cultivation techniques. The present invention provides a method for rapidly determining the
open pollination intensity of macadamia nuts. At the early flowering stage, pistils with slightly swollen ovaries are sampled, fixed with FAA and then sectioned. The sections are observed and the percentage of pistils PLS with
pollen tubes growing to the base of the style and the
ovary is counted. The
open pollination intensity of macadamia nuts is evaluated according to the value of PLS. From the start of
sample collection to the completion of
pollen tube
fluorescence microscopy detection, the present invention only takes 30 h to know the open
pollination intensity of all tested varieties. It has a small
workload, intuitive, accurate and reliable results, and little damage. It can quickly detect the
insect pollination efficiency in the current year for
macadamia nut plantations, provide a basis for whether to carry out artificial supplementary
pollination in the current year and reasonably place beehives, and can be widely used in
macadamia nut plantations on a large scale.