37 results about "Open pollination" patented technology

The invention relates to a method for inducing industrial pure female marihuana. Pins of a main stem and side branches of a female plant are sprayed twice with a silver thiosulfate (STS) solution by adopting a method so as to induce the female plant to generate male flower buds, the female plant is converted into monoecism, and set seeds after open pollination are industrial pure female marihuana seeds. The concentration of the STS solution is 0.2-2mmol/L and the solution is prepared by mixing AgNO3 and Na2S2O3 mother liquor according to the molar concentration of Ag<+> to S2O3<2-> of 1:4. Based on the situation that the industrial marihuana in China is dioecious, the chemical induction is carried out on the industrial marihuana in a special growing period to obtain an industrial pure female marihuana group. The method is easy to operate and low in cost, and has a relatively good application prospect in the aspects such as simplification of a hybrid seed production process of the industrial marihuana, development of selfing purification of the industrial marihuana, and the like.

The invention discloses an artificial pollination breeding method of a pinus massoniana hybrid. The artificial pollination breeding method comprises the following steps of 1, selection of masson pine as a female parent, 2, selection of pinus elliottii, pinus elliottii-pinus caribaea hybrid, pinus caribaea and pine moth-resistant pinus massoniana as male parents, 3, collection of male parent pollen, 4, treatment on the male parent pollen, 5, bagging and artificial pollination by a home-made pollination tool, and 6, cultivation. Through the artificial pollination breeding method, seeds of the pinus massoniana hybrid having the excellent and stable properties of straight trunk, fast early-stage growth rate, high rosin yield and pest and disease resistance are obtained. The artificial pollination breeding method solves the problems of open pollination in a pinus massoniana seed garden and improves crossbreeding efficiency. The artificial pollination breeding method is a complete artificial pollination breeding method, has simple processes, is practical, has a low cost, low requirements on operators, and small influences by a natural environmental factor such as weather, can effectively improve cross pollination efficiency in a pinus massoniana seed garden, can improve accuracy and reliability of cross pollination, can accelerate seed selection of a novel forestall germplasm resource variety, and has good economic, social and ecological benefits.

The invention discloses a construction method for synthesis of warm corn artificial population Suwan-Lancaster. The construction method comprises the following steps: selecting strains respectively from Suwan and Lancaster class groups, carrying out artificial purposeful hybridization for synthesizing the warm corn Suwan-Lancaster population, carrying out open pollination under natural isolation conditions, and continuously carrying out genetic equilibrium for four times to form Suwan-Lancaster original population C0, named Sulan C0. The warm corn artificially synthesized Suwan-Lancaster population has high yield genetic gain and great population structural variation; a half sib recurrent selection method is adopted for improving the Suwan-Lancaster population for three times; and meanwhile, line selection is directly carried out on the improved population, a batch of good inbred lines are bred, and the population has the effect of promoting widening of the existing corn germplasm foundation of China.

The invention discloses a rapid propagation method of radish breeding material. The method comprises the following steps: (S010), mesh enclosure setting up, namely respectively matching, assembling, connecting and fixing long rods, cross rods, vertical columns, three-way connecting rings and vertical column bases; (S020) radish parent material planting, namely respectively, sequentially and intensively planting male parent and female reproduced by parent of radish sterile line and radish hybridized combination according to a certain line number; (S030) radish flowering phase management, namely removing heteromorphic plant after transplanted radish plant survives, removing fertile plant and keeping sterile plant for a sterile line from radish bolting to an initial flowering phase, keeping completely fertile plant with typical shapes and normal floral organs for maintainer line and male parent line, and applying insecticide for one time 7 to 10 days before osmia is released; (S040) osmia release and management, namely putting osmia into a mesh enclosure in the initial flower stage of radish, ensuring that osmia performs open pollination, and recycling and storing the osmia after pollination; and (S050) seed harvesting. The method is simple to operate and low in cost, and can obviously improve the seed output.

The invention provides a one-hole-multiple-plants inbred line breeding method capable of enhancing the density-tolerant property and lodging resistance of maize. The method comprises the following steps that firstly, hybrid seeds obtained through hybridization of two excellent maize varieties serve as S0 generation of seeds, superior plants selected from the S0 generation to be inbred, and S1 generation of seeds are obtained; secondly, the S1 generation is planted in a one hole-multiple-plants mode at high density, female and male coordinated individual plants are inbred, and seed reservation is conducted on each inbred ear with the grain weight per ear in the top 10%; thirdly, the S2 generation is planted in the one hole-multiple-plants mode at high density according to ear rows, the female and male coordinated individual plants are selected in five holes at one end of each ear row to be inbred, the other individual plants are subjected to open pollination, and triple screening is conducted on the ear rows; fourthly, the S3 generation is planted, the S3 generation and 4-5 existing common inbred lines are subjected to test cross, multi-point testing is conducted on the test cross F1, screening is conducted according to high adaptability, good quality, ordinary yield and high combining ability, and the selected ear rows are the maize density-tolerant lodging-resistant inbred lines. By means of the method, the underground root system and aerial roots of the bred inbred lines are very developed, and the maize has good density-tolerant and lodging-resistant abilities.

The invention discloses a method for breeding onion male sterile line and maintainer line by utilizing molecular markers. A PCR (polymerase chain reaction) experiment is performed to detect single onion ball nucleoplasm genotype by utilizing specific primers (disclosed as SEQ ID NO.1-6) to screen a sterile strain and a maintainer strain onion ball, thereby implementing two-line matching. A plurality of sets of primers are designed according to Ms allele flanking sequences (F1890 and S1887) and onion cytoplasmic male sterility specific sequence mt472; and scientific experiments are performed to develop a molecular marker detection system and detection kit based on multiplex PCR. The detection system can distinguish 6 karyoplasm fertility types of onions through one PCR experiment; and the detection system is utilized to directly breed the sterile line and maintainer line from the open pollination colony, thereby implementing one-step matching of two lines. The development of the multiplex PCR detection system has important meanings in accurate and quick identification of onion nucleo-cytoplasmic interreaction male sterility karyoplasm genotype.

The invention relates to Sesamum indicum (sesame) plants comprising quantitative trait loci (QTL) associated with shatter resistant capsules and improved organoleptic properties and having a protein content of about 18% to about 25% by weight, a fat content of about 48% to about 56% by weight, and/or an L value of greater than 60 as measured, for example, by Hunter Colorflex color meter. Provided are sesame plants and seeds having these characteristics (both open pollinated and hybrids) as well as methods for breeding sesame plants, growing sesame plants, and food products made with the sesame plants and parts thereof, preferably the sesame seeds.

The invention discloses a method for breeding a high-yield shiny-leaved yellowhorn strain. The method comprises the following steps: collecting open pollinated seeds of shiny-leaved yellowhorn, identifying a male parent with an SSR (signal sequence receptor) molecule marker after the seeds germinate and screening mother plants with the self-compatibility of above 60%, with the fertile ovule rate of above 90% and with the seed yield per plant of above 30% higher than that of a contrast tree under same conditions; taking a sapling in a shiny-leaved yellowhorn container as a rootstock, grafting ascion of a selected single plant and identifying a male parent of a seed produced by the single plant; performing second generation grafting and identifying a male parent after the seed is verified as a selfing seed; determining that a mother plant is a final selection single plant if the seed is verified as the selfing seed again; taking a scion of the final selection single plant, taking a seedling of five years plantation of shiny-leaved yellowhorn as a rootstock, grafting the scion of the final selection single plant and expanding propagation, so as to obtain the self-fertility high-yieldshiny-leaved yellowhorn strain. According to the method, a self-fertility high-yield fine breed of the shiny-leaved yellowhorn is cultivated by adopting means such as the SSR molecule marker, seedling raising in the container, grafting and the like, so that the problem of lack of the high-yield fine breed of the shiny-leaved yellowhorn is solved.

Abstract of the Disclosure

An enhanced, diploid pollenizer watermelon plant and method used to maximize the yield of triploid seedless watermelons per area. The enhanced pollenizer watermelon plant of the invention is either a hybrid variety, an open-pollinated variety or a synthetic variety, that exhibits the characteristics of small leaves and fruit with a brittle rind that splits when the fruit is overripe or breaks when relatively small physical forces are applied. The watermelon plant of the invention is also preferably characterized by extended flowering duration, thereby increasing the number of triploid watermelon flowers that are pollinated and set fruit. The method for producing a seedless watermelon fruit, includes the steps of providing a pollenizer diploid watermelon plant, extending the duration of flowering of the pollenizer plant while reducing the number of such plants needed to pollenize the same number of triploid watermelon plants, and maximizing dispersal of the pollenizer watermelon plant throughout the field of triploid watermelon plants.

The invention relates to the field of selective breeding of wheat, and in particular discloses a selective breeding method of a high-yield and disease-resistant purple grain wheat line. The selective breeding method comprises the steps of carrying out backcross on purple grain wheat Luozhen No. 1 and dwarf-male-sterile wheat for a plurality of times to breed purple grain dwarf-male-sterile wheat; after that, carrying out crossbreeding through fully open free pollination by taking the purple grain dwarf-male-sterile wheat as a female parent and taking Zhoumai No. 22 and Jimai No. 22 as male parents to obtain purple grain hybrid F0 generation; screening the purple grains in the purple grain hybrid F0 generation for planting, and breeding the high-yield and disease-resistant purple grain wheat line by means of self-cross selective breeding of more generations, wherein the purple grain wheat grains of the last generation are selected for planting in the process of self-cross selective breeding. The method takes the Luozhen No. 1, the dwarf-male-sterile wheat, the Zhoumai No. 22 and the Jimai No. 22 as parents, and implements open pollination in the selective breeding process so as to enable genes to make full exchange; good genes and target shapes are effectively aggregated, so that a large number of outstanding single plants appear in the group, and the purple grain wheat progress is accelerated; the new purple grain wheat line obtained by selective breeding is high in yield and good in disease resistance.

The invention provides a method for rapid screening of garden establishment parent materials of a cedar seed garden. The method comprises the following steps: conducting open pollination on candidateparents of a garden establishment region in a control area; obtaining a filial generation of each parent after the open pollination through monophyletic seed collection; using an SNP molecular markerfor genotyping of the parents and the filial generations so as to determine the parents of which the filial generations are dominant; and determining the parent materials for garden establishment according to traits of blossom and growth. Forest trees have large genomes in general. The method rapidly detects characteristics of differences of genomes among forest plant individuals with the SNP molecular marker and is characterized by fast screening, a large processing amount, high polymorphism, high stability and a wide coverage scope.

The invention discloses a method for preparing synthetic hybrid seeds by utilizing a cytoplasmic male sterile line of kenaf. The method comprises the following specific steps of: (1) hybridizing the cytoplasmic male sterile line of the kenaf as a female parent with a male sterile maintainer line to reproduce the cytoplasmic male sterile line, hybridizing the cytoplasmic male sterile line as the female parent with a restoring line to prepare F1-generation hybrid seeds, and hybridizing the F1-generation hybrid seeds as a male parent with the cytoplasmic male sterile line of the female parent of the F1-generation hybrid seeds as the female parent so as to construct permanent separated groups; (2) carrying out mixed planting on the plurality of permanent separated groups, and selecting relatively consistent single sterile plants into one group to obtain a plurality of basic groups; (3) carrying out open pollination on all the groups, and after multiple generations, combining plant lines without separation in all the groups to obtain a plurality of groups with balanced inheritance; (4) identifying the groups with balanced inheritance and selecting the groups conforming to the breeding object, i.e., the synthetic hybrid seeds. The method disclosed by the invention has the advantages that the prepared synthetic hybrid seeds can be utilized by multiple generations, and farmers do not need to buy the hybrid seeds for every year, so that the planting cost of the kenaf is reduced.

The invention discloses a method for establishing a distant hybridization open pollination breeding system of stone fruits and belongs to the technical field of stone fruit breeding. The method comprises the following steps: 1, selecting parents; 2, selecting an environment; 3, removing pollinizers; 4, selecting tree types; 5, arranging the parent trees; 6, performing open hybrid pollination; 7, obtaining hybrid seeds; 8, accelerating germination and sowing; 9, performing grafting of high branches; 10, screening. According to the method, through parent selection of self-incompatibility and cross-compatibility, a large number of hybrid seeds can be obtained under natural conditions. Compared with an artificial pollination mode, the method has the advantages that the labor cost is greatly reduced, and the efficiency is remarkably improved.

The invention discloses a method for cultivating sea buckthorn with large fruit and high resistance, which is characterized in that a plurality of superior individual plants with large fruit and less thorn are selected from the wild Mongolian subspecies of sea buckthorn, and are configured with the female-male proportion of 10 : 1 for open pollination and hybridization; the generated progeny seedlings are cultivated; and a hybrid clone with small plants, large fruits without thorns and strong fruit fly resistance is selected and named as Sarin No.1. The sea buckthorn cultivated through the method has the advantages of fine variety and strong adaptability.