Method for rapid screening of garden establishment parent materials of cedar seed garden

A screening method and parent technology, applied in botany equipment and methods, applications, plant genetic improvement, etc., can solve the problems of unresolved seed orchard yield, low and unstable number of effective parents in seed orchard, and reach coverage Broad, high polymorphism, less work effect

Pending Publication Date: 2020-06-19
GUANGDONG ACAD OF FORESTRY
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Problems solved by technology

At present, forest seed gardens in various countries in the world have many problems such as low yield and instability, but they are still regarded as an indispensable means by the breeding community
[0003] The construction of Chinese fir seed orchards first started in the mid-1960s. At present, Chinese fir has entered the construction of the fourth generation o

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  • Method for rapid screening of garden establishment parent materials of cedar seed garden

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Embodiment Construction

[0021] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0022] The invention provides a fast screening method for the parent material of Chinese fir seed garden construction. The separation method comprises the following steps:

[0023] combine figure 1 As shown, step 1, grafting 18 candidate parents in the control area, marked as (S1, S2, S3, S4, S5, S6, S7, S8, S9, S10, S11, S12, S13, S14, S15, S16 , S17, S18).

[0024] Step 2, the above-mentioned 18 candidate parents pollinate freely after flowering.

[0025] Step 3, collecting 18 candidate parents to extract DNA from leaves of the current year for SNP single nucleotide polymorphism typing;

[0026] Step 4. Randomly select 81 individuals among 18 free-pollinated offspri...

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Abstract

The invention provides a method for rapid screening of garden establishment parent materials of a cedar seed garden. The method comprises the following steps: conducting open pollination on candidateparents of a garden establishment region in a control area; obtaining a filial generation of each parent after the open pollination through monophyletic seed collection; using an SNP molecular markerfor genotyping of the parents and the filial generations so as to determine the parents of which the filial generations are dominant; and determining the parent materials for garden establishment according to traits of blossom and growth. Forest trees have large genomes in general. The method rapidly detects characteristics of differences of genomes among forest plant individuals with the SNP molecular marker and is characterized by fast screening, a large processing amount, high polymorphism, high stability and a wide coverage scope.

Description

technical field [0001] The invention relates to the technical field of forestry, in particular to a rapid screening method for parent materials for constructing Chinese fir seed orchards. Background technique [0002] Chinese fir( Cunninghamia lanceolata (Lamb.) Hook.) has survived on the earth for 700,000 years and has been exploited and utilized by humans for more than 2,000 years. Fir has many advantages such as fast growth, good material properties, and wide application. Its distribution range is very wide, covering 17 subtropical provinces (regions) in my country. The tree seed orchard is a plantation established from artificially selected excellent tree clones or progeny families, and it is an important link in tree breeding. Since the 1960s, forest seed gardens have been generally established in countries all over the world, and my country has established fir clone seed gardens since 1964. According to incomplete statistics, more than 50 countries have established ...

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Application Information

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IPC IPC(8): A01H1/04A01H1/02A01G2/30
CPCA01H1/04A01H1/02A01G2/30
Inventor 何祯祥王明怀张应中李小川邓智曦林军赖旭恩崔春意邓文剑刘晓东
Owner GUANGDONG ACAD OF FORESTRY
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