Method for rapid screening of garden establishment parent materials of cedar seed garden
A screening method and parent technology, applied in botany equipment and methods, applications, plant genetic improvement, etc., can solve the problems of unresolved seed orchard yield, low and unstable number of effective parents in seed orchard, and reach coverage Broad, high polymorphism, less work effect
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[0021] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.
[0022] The invention provides a fast screening method for the parent material of Chinese fir seed garden construction. The separation method comprises the following steps:
[0023] combine figure 1 As shown, step 1, grafting 18 candidate parents in the control area, marked as (S1, S2, S3, S4, S5, S6, S7, S8, S9, S10, S11, S12, S13, S14, S15, S16 , S17, S18).
[0024] Step 2, the above-mentioned 18 candidate parents pollinate freely after flowering.
[0025] Step 3, collecting 18 candidate parents to extract DNA from leaves of the current year for SNP single nucleotide polymorphism typing;
[0026] Step 4. Randomly select 81 individuals among 18 free-pollinated offspri...
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