70 results about "Tree breeding" patented technology

The invention relates to 'a bark grafting method of apocarya' and belongs to the field of fruit trees and forest tree breeding. The method includes cutting a stock or a lignification branch of the apocarya in vigorous growth to the xylem, conducing inclining cutting on the portion above a cut to form a 45-degree cut, cutting downwards on an edge bark layer of the cut through a stem grafting knife for 3cm with cutting length as 3cm and cutting width determined by scion thickness and conducting scion branch processing and grafting. By means of the method, summer grafting survival rate of scions dormant in winter is greatly improved, the problem of scion grafting survival of ligneous plants like the apocarya which is introduced from the southern hemisphere in summer is effectively removed, and simultaneously grafting period of the apocarya can be prolonged.

The invention discloses an under-tree broiler chicken breeding method. The under-tree broiler chicken breeding method includes the following steps of selecting a high-quality-broiler-chicken under-tree stocking field; selecting a stocking house; selecting under-tree stocking high-quality broiler chicken varieties; managing high-quality-broiler-chicken under-tree breeding and feeding; selecting the formula of under-tree breeding feed; building a rotation grazing system of the forest land grazing field, and planting grazing; researching stocked chicken immune procedures, and selecting the brooding-stage immune procedure; controlling common parasitic diseases of high-quality-broiler-chicken under-tree stocking; conducting pollution-free treatment on chicken stocking waste.

The invention discloses a method for improving the germination percentage of Cyclocarya paliurus seeds, and belongs to the tree breeding field. The method is characterized in that the method comprises the steps of alkalization seed soaking treatment, oscillation pressure-reduction nitrogen-enriched treatment and germination accelerating treatment. Compared with the prior art, the method has the advantages of promotion of the germination of the Cyclocarya paliurus seeds, high germination percentage, and high seedling survival rate at low temperatures.

Important aims in nearly all tree-breeding programs around the world are to produce plants with increased growth rates and stem volumes, and shorter rotation times. Such trees would yield more biomass per area unit. Here the present inventors have shown that when over-expressing a key regulatory gene in the biosynthesis of the plant hormone gibberellin (GA) in hybrid aspen (Populus tremula x P. tremuloides), improvements in valuable traits such as growth rate and biomass are obtained. In addition, these trees also have longer xylem fibers than unmodified wild type plants. Long fibres are very desirable in the production of strong paper, but it has not (as yet) proved possible to influence this trait by traditional breeding techniques. A further advantage of the present invention is that it may reduce or eliminate the use of growth influencing chemicals in forestry.

The invention discloses a new application of a cotton and rape brassinolide synthetase GhDWF4 gene. The GhDWF4 gene can promote the extension of hypocotyls of plants and can also enlarge areas of plant leaves and increase the yield of fruits so as to promote the growth of the plants if being expressed in the plants, and the GhDWF4 gene can be used for crop breeding and forest tree breeding, thereby enhancing the vegetative growth of transgenic plants and increasing the biological yield of the plants. The invention also provides a plant expression vector which contains the GhDWF4 gene and a transformant thereof and provides a method for cultivating transgenic plants which contain the GhDWF4 gene. The invention has the advantages that the vegetative growth and the reproductive growth of the transgenic plants are promoted, the heights of the transgenic plants are increased, and the yield of fruits is increased.

The invention relates to a date tree manual control crossbreeding method assisted by molecule, that is, the hybrid progeny of the date tree is obtained by artificially selecting parent, cover net control hybridation, pollination assisted by insects, molecular marker hybrid identification, etc. According to target breeding, the parent is selected, two parents are adjacently planted or grafted on the same date tree; before the plants of the parents blossom out, a net that has the functions of ventilation and light transmission but can stop pollination insects from entering is used for completely covering a parent tree, the pollination insects that never collect pollen are put in the net for helping pollination, and the pollination insects and the net cover are removed after florescence is over; the fruit born by the two parents are all picked up, the kernels are taken out and sowed to obtain seedlings; the genome DNAs of all the seedlings and the two parents are extracted, the molecular marker techniques such as AFLP, etc., are adopted for hybrid identification; the seedlings of the hybrid progeny are systematically observed and evaluated for breeding improved varieties. The method is applied to the practices of date tree breeding, can break through the bottleneck of difficult emasculation in the crossbreeding of date trees and improve the idioplasm innovation and crossbreeding efficiency of the date trees.

The invention discloses a Chinese yew tree high position grafting method, belonging to the forest tree breeding technology field. The Chinese yew belongs to the dioecism plant. The Chinese yew tree high position grafting method comprises steps of adopting a flowered male plant as a stock, snipping a branch of a flowered and fruited fine female plant as a scion, performing cut-grafting and canopy replacement on a main branch on an upper part of a rootstock tree to change an original breed, which is also called breed changing, and maintaining one to three rootstock top shoots as pollination branches. Compared with the prior art, the Chinese yew tree high position grafting method adopts the branch of the fruited Chinese yew fine female tree to graft on the upper part of the flowered male plant to realize high position grafting and breed changing; the grafting living rate achieves more than 90%; the grafted Chinese yew fruits next year and enters a fruit high yield period in a third year; and each plat can produce fresh fruits more than 20 kilo. The Chinese yew tree high position grafting method solves a problem that a male tree and a female tree are different plants, realizes a monoecious plant and can maintain good characters of the female tree; three years later, the grafted tree begins producing a large quantity of fruits after grafting, six years earlier than the seedling Chinese yews, which improves multiple times of the production.

The invention discloses a molecule marker, a screening method, a kit and an application for screening a stress-resistant poplar. The molecule marker is detected through an MSAP method and includes MS1, MS2, MS3, MS4, MS5, MS6, MS7, MS8, MS9 and MS10. By means of the molecule marker determined in the invention and for screening the stress-resistant poplar, plants which are strong in adversity stress adaptability and are high in vitality can be screened out in an early stage during growth of the poplars and a forest tree breeding selection period is greatly reduced. The invention has important strategic significance in development of a poplar fast-growing high-pulp-yield forest and of ensuring that a high-yield industrial pulp raw material can be provided stably and continuously in forestry in our country.

The invention discloses miR156 and application of a related biomaterial thereof in regulating disease resistance of plants. Transgenic miR156 white birch is used as a research object, wild type whitebirch serves as a control, the activity of superoxide dismutase, catalase, peroxidase, polyphenol oxidase and phenylalanine ammonialyase is determined by detecting the speck number after diseases occur, and the effects of miR156 on damage degree of disease infected plants is researched in the physiological aspect; by analyzing the transgenic miR156 white birch and disease-resistant related genes in the control, regulation of miR156 on disease resistant ways of plants is further proved. The miR156 has great significance in disclosing disease resistant functions of the miR156 and breeding disease-resistant white birch varieties and contributes to enrichment of forest tree breeding resources.

The invention discloses a seed soaking agent for promoting the germination of Folium Cylocaryae Paliuri seeds, and belongs to the tree breeding field. The seed soaking agent is characterized in that the liquid seed soaking agent is prepared by adding 0.6g of NaCl, 0.98g of KCl, 0.17g of CaCl2.2H2O, 0.11g of MgCl2.6H2O, 30-40mg of poly(ethyleneglycol)alkylmethacrylate, 40mg of gamma-aminobutyric acid and 0.2g of astaxanthin to 1000ml of distilled water. Compared with seed soaking agents in the prior art, the seed soaking agent in the invention can promote the germination of the Folium Cylocaryae Paliuri seeds, so a high germination percentage is obtained, and a high seedling survival rate can be obtained at a low temperature.

The invention discloses an idesia polycarpa superior tree breeding method, and solves the problem that idesia polycarpa is high in yield but idesia polycarpa oil is not high in quality in the prior art. According to the idesia polycarpa superior tree breeding method, the idesia polycarpa is subjected to superior tree selection first and then a superior tree is bred by adopting grafting breeding; the method specifically comprises the following steps: superior tree pre-selection, superior tree re-selection, superior tree final selection, and grafting propagation. The method disclosed by the invention is simple and convenient to operate; idesia polycarpa superior tree breeding is performed by adopting the method disclosed by the invention, so that obtained idesia polycarpa tree good variety is high in yield, and the idesia polycarpa oil is good in quality.

The invention discloses a construction method for a breeding pedigree of lutraria sieboldii. The lutraria sieboldii is a rare bivalve shellfish, and excessive breeding causes problems such as germplasm degeneration and frequent diseases; and pedigree breeding becomes a first choice for industrial development, but because of a subtidal open water area breeding environment of the lutraria sieboldii,complex resources, and a large number of individuals to be marked, common marking and molecular marking are difficult, and an effective pedigree breeding mechanism is not established in China. The method disclosed by the invention adopts the technical steps of artificial directional insemination, breeding, pedigree individual marking and cultivation to solve the difficult problem of pedigree construction of pedigree breeding of the lutraria sieboldii, can quickly realize the pedigree breeding by combining genetic polymorphism analysis, and provides technical references for pedigree breeding of the lutraria sieboldii and related subtidal bivalve shellfish.

The invention relates to a cultivation method of cluster Chinese tallow trees, the cultivation method comprises the steps that a sodium azide mutagenesis solution is added into Chinese tallow tree seeds, then the seeds are planted in a salt pond, salt-tolerant mutants are screened out, irrigation and fertilization are conducted on seedlings in the mode of leaning one sides, and root systems of theseedlings are distributed in oval shapes with sparse ends and flourishing ends in soil balls; and a plurality of seedlings are circumferentially arranged in sequence and spliced and clustered again,large roots at the sparse ends of the soil balls are crossed and spliced, and then clustered Chinese tallow trees with good conditions are obtained through clustered shaping, supporting and fixing, pruning and shaping and regular maintenance. According to the cultivation method, sodium azide is selected as a salt-tolerant clustered Chinese tallow tree breeding mutagenic agent, damage to mutagenicseeds is small, salt-tolerant genes of Chinese tallow trees can be fully expressed, and drought resistance and dry hot air resistance can also be fully expressed; then irrigation and fertilization areconducted on the seedlings in the mode of leaning one sides, so that the sparse ends and the flourishing ends are generated at the roots of the Chinese tallow tree seedlings; and then, soil ball digging and cutting are carried out on the Chinese tallow tree seedlings, and splicing planting is carried out with dense seams, so that the spacing among the seedlings is effectively reduced, bending andcompressive death of the seedlings are avoided, and the ornamental effect of round crowns of the clustered Chinese tallow trees is guaranteed.

The invention provides a top-grafting method for weeping trees, and relates to the technical field of tree breeding. The top-grafting method for the weeping trees comprises the following steps of selection and treatment of stocks; selection and treatment of scions; top grafting of the weeping trees; management after top grafting; water and fertilizer management. In the top-grafting method for theweeping trees, the selected scions are soaked with a treatment agent, the anti-bacterial and disease-resistant ability of the scions is improved, the situation that the scions are infected with pathogenic bacteria to result in low surviving rate is prevented, the activity of cambiums is promoted, cell division wound healing is facilitated, the callus growth is accelerated, and moreover, the surviving rate of the top-grafting trees is greatly improved.

The invention relates to an underground cicada nymph breeding device. It solves technical problems such as unreasonable design of the prior art. It includes a number of cultivation cylinders buried horizontally in the soil and located on the periphery of the tree. The cultivation cylinder is in the shape of a hollow arc-shaped tube with a cultivation cavity inside. The two ends of the cultivation cylinder are respectively open. It is a closed structure with the opening closed, and each cultivation cylinder is arranged in sequence in the circumferential direction, and two adjacent cultivation cylinders are connected by a detachable structure so that each cultivation cylinder is spliced ​​into a ring structure. The access holes connected with the cultivation cavity are provided with a plurality of partition plates located in the cultivation cavity in the cultivation cylinder, and at least one access hole is corresponding between two adjacent partition plates. The method has the advantages that: by placing the cicada egg branches with the cicada nymph seedlings in the cultivation cylinder, the cicada nymphs can be managed in a centralized manner and the survival rate of the cicada nymph seedlings can be improved.

The invention relates to a method for promoting rapid germination of seeds of acer griseum, and a seedling-raising method of the acer griseum, belonging to the technical field of forest tree breeding. The method for promoting the rapid germination of the seeds of the acer griseum comprises the following steps: (1) soaking samara of the acer griseum into a potassium permanganate solution for 30-60min, then washing the samara with sterile water, putting the washed samara into a sterile container, and sealing the container by using a breathable film; (2) taking out embryo of the samara, obtained in the step (1), and soaking the embryo into a gibberellin solution for 2-3h; (3) carrying out bactericidal treatment on the embryo soaked in the step (2), and then culturing for promoting germination. The obtained germinating seeds are raised into seedlings in cultivating bags or cultivating holes. After the method is adopted, the seeds of the acer griseum are rapid in germination speed and high in germination rate; the methods provided by the invention are convenient to operate and easy to popularize and apply, and are free of special nursing, thus having good application prospect.

The invention provides a method for raising seedlings of cherry trees and belongs to the field of tree breeding. The method comprises: 1) acquiring explants of the stem segments of a cherry tree, 2) inducing and cultivating the explants of the stems of the cherry tree, 3) proliferating and cultivating multiple shoots of the cherry tree, 4) carrying out rooting culture of the cherry trees through afirst stage: culturing the single bud cut from the multiple shoots through a first rooting medium and carrying out culture under 2500-2800 Lux and illumination for 20h every day for 7 days and a second stage: transferring the seedling to a second rooting medium and carrying out rooting culture for 25-30d until good roots grow, 5) hardening and transplanting tissue culture seedlings of the cherrytree through hardening seedlings at the normal temperature for 7-14d, and transferring the seedlings into the matrix and carrying out culture for 25-30d, and 6) transplanting the tissue culture seedlings in a culture field. Compared with the traditional cherry tree grafting or cutting propagation method, the method saves labor and time, realizes a low cost, is easy to operate, realizes the survival rate of cherry tree seedlings of 97%, and can realize large-scale standard production.

The invention discloses a cutting propagation method for Carpinus tientaiensis, and belongs to the technical field of forest tree breeding. The cutting propagation method is characterized in that thecutting propagation method mainly comprises the steps of cutting shoot preparation, root growth processing, substrate preparation, cutting, root growth process management, transplantation, later-period management and the like, wherein a cutting shoot is from a 3-5-year-old Carpinus tientaiensis seedling. According to the cutting propagation method, the technical problem that the Carpinus tientaiensis cutting is extremely difficult to grow roots can be solved. After measures, including seedling adoption, refrigeration, NaOH solution processing, rooting agent processing, seedbed warming and thelike, a great quantity of cutting shoots successfully grow roots and form seedlings, and after activating treatment is added, a cutting shoot rooting percentage and a planting percent are independently 83.31% and 80.67%.

The invention discloses a method for efficiently separating lignin based on dilute acid preimpregnation. The method comprises the following steps: a, removing impurities from a biomass raw material, crushing, and drying; b, impregnating the biomass raw material with an aqueous dilute acid solution, then lyophilizing, and then performing ball-milling and crushing to obtain ball-milled biomass; andc, performing hydrolytic treatment on the ball-milled biomass obtained in the step b by using composite cellulose to remove carbohydrates from the biomass and obtain lignin in a form of residue, and repeatedly washing the residue to obtain a high-purity enzymolysis residue, namely the lignin. The invention provides an environmentally-friendly and high-efficiency lignin separating method; the environmentally-friendly and high-efficiency lignin separating method can be used for preparing high-purity and structurally intact lignin and is directly applied to structural characterization; a novel method is provided for interpreting the original lignin structure in the biomass, and a theoretical basis and a technical support are also provided for forest tree breeding and further high-value application of the lignin.

The invention discloses a forest tree precise breeding method and application thereof, and the method comprises the following steps: soaking seeds of different trees before sowing, and then sowing and raising seedlings of the seeds; after the seedlings grow to 0.4-0.6 m, selecting saplings with thick and strong trunks and multiple branches, soaking the roots of the saplings in a compound microbial agent for 20-40 minutes, and then transferring the saplings into heavy metal polluted soil for mixed planting. By means of the breeding method, the forest trees capable of repairing the heavy metal contaminated soil can be accurately screened out, growth of the forest trees is promoted, and the heavy metal contamination degree of the soil is improved.