62 results about "Tree breeding" patented technology

The invention discloses a pest control management system and method for forest tree breeding, and relates to the technical field of forest tree breeding and seedling raising, and the method comprises the following steps: collecting environment data and soil rhizosphere data by collecting multi-mode images of leaves and trunks of a pinus massoniana young forest and videos that pinus massoniana caterpillars gnaw leaves and cluster diffusion; constructing a disease and insect pest recognition model by using a convolutional neural network, performing definite diagnosis on pine wood nematode disease, pine caterpillars and monochamus alternatus hope, and quantifying the disease and insect pest occurrence area and population density of each sub-region; calculating a pine wood nematode disease control demand index, comparing the pine wood nematode disease control demand index with a threshold value, and triggering a corresponding control strategy; the diffusion coefficient of the pine caterpillars is calculated and compared with a risk threshold value, and a prevention and control scheme is formulated; calculating the propagation coefficient of the monochamus alternatus hope, judging the propagation risk and giving a prevention and control strategy; according to the invention, intelligent pest identification, risk quantification and control strategy closed-loop control are realized, treatment and scientific prevention and control are facilitated, and the pest control efficiency of the pinus massoniana young forest and the forest stand health level are improved.

The invention provides a tree species screening method for high-altitude drought afforestation, an afforestation method and application, and belongs to the technical field of forest tree breeding and cultivation. According to the screening method, five physiological and biochemical indexes of soluble protein, malondialdehyde, proline, peroxidase and soluble sugar in the tree species are measured through a gradient drought stress experiment, and comprehensive scoring is performed by adopting a membership function, so that the drought-resistant tree species are scientifically screened. The tree species such as sophora moorcroftiana, rosa serrulata and amorpha fruticosa screened by the method are applied to afforestation in the high-altitude arid region, and the afforestation survival rate and preservation rate can be remarkably increased. The invention provides a reliable technical scheme for solving the long-term problem that the selection of tree species in the difficult site field lacks scientific basis.

The invention relates to the field of genome prediction, and particularly discloses a GEP-GANGP-based genome prediction system and method, and the method comprises the steps: generating synthetic phenotype data by using GEP-cGAN; processing real and synthetic data through a feature extraction network; calculating interaction characteristics of the genotype and the environment by adopting a cross-modal attention mechanism; a transfer learning module is used for realizing knowledge transfer and feature enhancement; synchronously outputting a phenotype prediction value, an effect weight and an interpretability characteristic through a multi-task decoder; the system comprises a GEP-cGAN module, a biological multi-head attention mechanism BMAM module, a cross-modal interaction feature extraction module, a transfer learning TrG2P module and a multi-task decoder module. According to the method, the problems of low prediction precision, poor generalization ability and weak model interpretability in a small sample scene in forest tree breeding are solved, the accuracy and practicability of genome prediction are remarkably improved, and reliable technical support is provided for forest tree genetic improvement.

The application provides a large-scale rapid tree planting device and method for forest tree breeding, which comprises a mobile vehicle body, a first circular hole is symmetrically formed in the mobile vehicle body; a lead screw motor is symmetrically installed on the mobile vehicle body; a lifting plate is slidably connected to the mobile vehicle body, and a lead screw of the lead screw motor is threadedly connected to the lifting plate; and a rotating cylinder is symmetrically rotatably connected to the lifting plate. Through the cooperation of the rotating cylinder, the first spiral blade, the conical plate and the second spiral blade, the tree seedling placing and soil covering processes can be automatically completed during the digging process. Specifically, the rotating cylinder is driven to rotate by the rotating assembly, the conical plate is controlled to open and close by the unfolding assembly, the tree seedling is accurately placed into the hole, and the soil covering assembly automatically backfills the soil. The series of actions are seamlessly connected, the continuity is high, the overall planting efficiency can be improved, and the device is suitable for large-scale rapid tree planting operation.

The application discloses a kind of saline-alkali resistant kandelia candel tree breeding soil salt content detection adjusting equipment, it is related to kandelia candel tree breeding technical field, it solves equipment when using, it cannot be automatically adjusted according to salt content, and when soil is watered to adjust salt content, it is usually watered to soil outside, moisture is difficult to soak to inner layer, leading to the adjustment of soil salt content has error, and device is difficult to use in combination, and the problem of large space occupation.A kind of saline-alkali resistant kandelia candel tree breeding soil salt content detection adjusting equipment, including breeding component, the rear side of the breeding component is equipped with adjusting component, the both sides of the adjusting component are respectively equipped with fresh water tank and salt water tank.The application is cooperated by flow pipe and mounting framework, so that water is directly discharged into the soil inside through the flow pipe, then sprayed outward through the spray head and enters the soil inside through the surface of the filter screen, so that the device can directly spray water inside the soil to prevent the situation that water does not penetrate into the soil inside.

The application provides a method for improving the germination rate and storage capacity of kiwi pollen, and belongs to the technical field of forest tree breeding. The method comprises the following steps: S1, placing the pollen of male kiwi flowers in 24-28 DEG C light treatment for 10-14 hours; S2, spreading the pollen treated in S1 on an in vitro germination medium for light culture; S3, observing the germination state of the pollen and calculating the survival rate of the pollen. According to the method, the pollen is first placed in 24-28 DEG C light pretreatment for 10-14 hours, so that the germination rate of the pollen is significantly higher than that of fresh pollen, and compared with the pollen without pretreatment, the method can further improve the pollen storage capacity of kiwi pollen under 4 DEG C and-20 DEG C storage conditions, and the decrease range of the pollen germination rate during storage is significantly reduced.

The invention provides a large-scale rapid tree planting device and method for forest tree breeding, and the device comprises a mobile vehicle body which is symmetrically provided with first round holes; the screw rod motors are symmetrically mounted on the mobile vehicle body; the lifting plate is slidably connected to the movable vehicle body, and a lead screw of the lead screw motor is in threaded connection with the lifting plate; and the rotating cylinders are symmetrically and rotationally connected to the lifting plate. Through cooperation of a rotating cylinder, a first spiral blade, a conical plate and a second spiral blade, sapling throwing and soil covering processes can be automatically completed in the pit digging process, specifically, the rotating cylinder is driven to rotate through a rotating assembly, opening and closing of the conical plate are controlled in combination with an unfolding assembly, saplings are precisely thrown into pits, and the pit digging efficiency is improved. Meanwhile, the soil covering assembly automatically backfills the soil, the series of actions are seamlessly connected, the continuity is high, the overall planting efficiency can be improved, and the device is suitable for large-scale rapid tree planting operation.

The utility model relates to the technical field of forest tree breeding devices, and discloses a forest tree breeding device with an adjustable spraying angle, which comprises a main body, soil is arranged on the inner side of the bottom of the main body, a water seepage diversion pipe is fixedly connected to the inner side of the bottom of the main body, and a water pump is fixedly connected to the left side of the water seepage diversion pipe. A water tank is fixedly connected to the left side of the water pump, a flexible hose is fixedly connected to the top of the water seepage flow dividing pipe, a lifting mechanism is arranged at the top of the main body, and a worker can quickly adjust the horizontal rotating angle of a spraying head through a rotating angle adjusting mechanism and a manual rotating worm; the support rotates with the connecting point of the connecting rod and the rotating column as the axis, so that the rotating vertical angle of the spraying head on the support can be rapidly adjusted, the device can accurately spray the pesticide to a target area, uneven pesticide receiving of forest trees is avoided, the control effect is improved, and waste is avoided.

The invention discloses an accurate pest expelling device for forest breeding, which comprises a monitoring station and a moving device, the upper end of the monitoring station is fixedly connected with a camera, the monitoring station is internally provided with an information assembly, a spraying assembly and an evaluation assembly, the moving device is provided with a shell, a feeding bin and an arc-shaped rail, and the arc-shaped rail is slidably connected with the evaluation assembly; through alternate use of two kinds of reverse pheromones built in the information assembly, pest expelling and luring effects can be achieved in different time periods, pests are caught into a collecting barrel in cooperation with the spraying assembly, then the situation of pest reproduction is effectively avoided and reduced, the caught pests can be collected through the evaluation assembly, meanwhile, a moving device is linked, and the pest trapping effect is improved. The general variety and number of pests are recognized through the recognition head, meanwhile, the distribution conditions of the pests in different areas can be obtained in cooperation with the camera, so that hostile organisms are automatically discharged, biological targeted releasing and repelling are conducted, and the pest repelling precision is improved.

The invention discloses an ecological tree pool facilitating forest tree breeding, and relates to the field of forest tree breeding. The ecological tree pool facilitating forest tree breeding comprises an ecological tree pool body, a net plate fixedly connected to the bottom of the ecological tree pool body and a plurality of mounting holes formed in the surface of the net plate, movable sleeves are mounted on the hole walls of the mounting holes in a matched mode, and a plurality of fixed sleeves are fixedly connected to the surfaces of the movable sleeves; the movable sleeve is communicated with a plurality of fixed sleeves, a plurality of spray heads are fixedly connected to the surfaces of the fixed sleeves, convex blocks are fixedly connected to the hole walls of the mounting holes, spiral grooves are formed in the outer sides of the fixed sleeves, the convex blocks are in sliding connection with the spiral grooves, and first springs are arranged on the outer sides of the fixed sleeves in a sleeving mode; according to the method, water is supplied to the roots while soil in the ecological tree pool is loosened, so that root development is guaranteed, and the forest tree breeding effect is further guaranteed.

The invention provides a withered stump removing device for forest tree breeding and a using method thereof.The withered stump removing device comprises a supporting frame, moving wheels, a hydraulic rod, a lifting rod and a clamping mechanism, the symmetrical moving wheels are arranged at the bottom of the supporting frame, one end of the lifting rod is rotationally connected to the upper end of the supporting frame, and the other end of the lifting rod is connected with the clamping mechanism; the hydraulic rod is fixed in the supporting frame, and the movable end of the hydraulic rod is connected to the middle of the lifting rod through a hinge seat. The closed annular area formed by the first tooth clamp and the second tooth clamp is adjusted through the adjusting assembly so as to adapt to the size of a withered tree stump, compared with an existing pulling device, the size of the clamping mechanism can be conveniently adjusted, the clamp does not need to be repeatedly replaced, and the convenience and efficiency of tree stump pulling work are improved.

The invention relates to the technical field of forest tree breeding and cultivation, in particular to an integrated method for improved variety breeding, rapid propagation and container seedling cultivation of poplar pie suitable for semi-arid regions. The integration method comprises the following steps: hybridizing populus arabinosa or populus sinkiang as a female parent and populus tomentosa or populus arabinosa as a male parent, screening by using a molecular marker to obtain F1-generation plants with salt-tolerant and drought-resistant characteristics, and sequentially carrying out rapid propagation, container seedling cultivation and afforestation planting on the F1-generation plants. Compared with the prior art, the method can solve the problem of rooting of the poplar pie, the afforestation survival rate is remarkably increased, and the method has the advantages of shortening the breeding period and accurately improving the stress resistance.

The application relates to the technical field of irrigation devices, and discloses an irrigation device for forest tree breeding and a use method thereof, which comprises a total water delivery pipe, branch pipes fixedly connected to the total water delivery pipe, an adjusting part arranged on the branch pipes, a limiting part arranged on the adjusting part, a protection part arranged on the total water delivery pipe, an adjusting assembly arranged on the branch pipes, a reset assembly arranged on the adjusting assembly, and a sealing ring one fixedly connected to the inner wall of the branch pipes. The adjusting part is arranged, so that the problem that the existing forest tree breeding irrigation device is difficult to irrigate according to the water requirement of saplings in the using process, which leads to the root rot of part of saplings and affects the normal growth and development of the forest seedlings is solved.

The invention discloses a poplar transcription factor PagERF106, a coding gene and application of the poplar transcription factor PagERF106 to improvement of drought resistance of plants, and relates to the technical field of biology. The amino acid sequence of the poplar transcription factor PagERF106 is as shown in SEQ ID NO. 2. Researches find that the PagERF106 gene is overexpressed in a plant body, and the drought resistance of the plant can be remarkably improved. The overexpression of PagERF106 can significantly up-regulate the expression levels of drought stress related genes such as P5CS, DREB46, RD22, RD29 and the like, and further strengthen the drought resistance mechanism of plants. As a key transcription factor of poplar responding to drought stress, the gene provides important reference for analyzing a plant drought-resistant molecular mechanism, also provides an efficient and feasible way for creating novel plant drought-resistant germplasm resources through genetic engineering, and has important application value in forest breeding and ecological restoration in arid regions.

The invention belongs to the technical field of forest tree breeding and seedling raising, and particularly relates to a method for efficiently screening rubber tree germplasm resources with strong drought resistance on a large scale, which comprises the following steps of: observing a nursery stock group when a nursery stock grows to a seedling or a clonal seedling with three canopy leaves and canopy leaves are in a stable growth period; by taking a plant or a clonal line as a unit, observing whether the length of a lobular pillow of a leaf at the middle lower part of a second leaf canopy of the nursery stock from the top leaf canopy to the top and the space between three lobular leaves are separated or not as primary selection judgment character indexes; primarily selecting germplasm resources with strong drought resistance according to conditions of short and thick lobule pillows and non-separation of three lobule spaces at the same time; carrying out natural drought stress on the primarily selected germplasm resources, then carrying out secondary selection, and judging the plants of which only the tops of canopy leaf stems have a small number of wrinkles and the large petioles do not become soft or droop as germplasm with strong drought resistance. According to the method, the drought resistance of the rubber tree germplasm resources can be identified in a high-efficiency, low-cost, large-scale and precise manner, the identification and discrimination accuracy is up to 90% or above, the drought resistance identification time can be greatly shortened, and the drought resistance breeding process of the rubber trees is accelerated.

The invention discloses a method for transforming citrus by using a drosophila Drosocin gene to induce antibacterial peptide expression resistance to citrus huanglongbing, and relates to the technical field of forest breeding and seedling raising, the method comprises the following steps: introducing the drosophila Drosocin gene into a citrus receptor material, and inducing Drosocin antibacterial peptide expression to obtain a citrus huanglongbing-resistant citrus material, and the nucleotide sequence of the drosophila Drosocin gene is as shown in SEQ ID NO: 1. According to the invention, the Drosocin gene is introduced into a citrus receptor material to induce the expression of Drosocin antibacterial peptide, so as to obtain a citrus material capable of resisting Candidatus Liberobacter asiaticum, thereby achieving the purposes of inhibiting the proliferation of Candidatus Liberobacter asiaticum and providing a green and efficient Candidatus Liberobacter asiaticum prevention and control means.

The application provides a new rubber tree breeding material induction method based on budding, comprising the following steps: S1, selection of rootstock varieties and scion varieties; S2, juvenile of the rootstock varieties and the scion varieties; S3, preparation of the rootstock and the scion; S4, budding; S5, screening of the budding mutation material: phenotype identification screening, stress resistance identification screening or molecular identification screening; the application selects rubber tree varieties with complementary traits as the rootstock and the scion, effectively improves overall traits, gradually induces target traits of the scion through multiple budding of the juvenile bud strip, effectively optimizes the target traits, and improves trait stability; the target budding mutation plant is quickly and accurately screened through molecular identification screening, stress screening and phenotype identification screening, the new variety breeding process is accelerated, the breeding period is shortened, and the thought for creating and widening new rubber tree breeding materials is widened.

This invention provides a device for removing dead tree stumps used in forest tree breeding and its method of use. The device includes a support frame, movable wheels, a hydraulic rod, a lifting rod, and a clamping mechanism. Symmetrical movable wheels are arranged at the bottom of the support frame. One end of the lifting rod is rotatably connected to the upper end of the support frame, and the other end is connected to the clamping mechanism. The hydraulic rod is fixed inside the support frame, and its movable end is connected to the middle of the lifting rod via a hinge. The closed annular area formed by the first and second toothed clamps is adjusted by an adjusting component to fit the size of the dead tree stump. Compared with existing removal devices, this invention allows for convenient adjustment of the clamping mechanism size without repeated clamp replacements, improving the convenience and efficiency of tree stump removal.

The invention discloses an experimental device for fruit tree breeding, and relates to the technical field of forest tree breeding, and the experimental device comprises a planting assembly which comprises a planting box cylinder wall; the supporting assembly comprises a T-shaped circular rail fixedly installed on the cylinder wall of the planting box, a sliding base is arranged outside the T-shaped circular rail, a limiting wheel is rotationally connected to one side, located below the inner edge of the T-shaped circular rail, of the sliding base, a limiting arm is fixedly connected to the side, away from the limiting wheel, of the sliding base, and a limiting bolt is in threaded connection with the bottom end, located below the outer edge of the T-shaped circular rail, of the limiting arm; the top of the sliding base is fixedly connected with a supporting rod. Through the quick assembly and disassembly effect between the T-shaped circular rail and the sliding seat, the situation that supporting cannot be completely achieved due to the fact that the position and number of a fixed support cannot be changed at will is avoided, and through the circular rods arranged in a fan shape and a rigid structure, the situation that in the prior art, the bending strength of a rubber plate is difficult to maintain or the growth direction of branches is difficult to correct is avoided.

The invention relates to the technical field of forest tree breeding, in particular to a rosin tree seedling raising device and a layered temperature control cultivation method.The rosin tree seedling raising device comprises a device body, the device body is formed by splicing a plurality of seedling raising disc bodies, the distance between two inner disc wall plates is relatively close, and a compact planting space is provided for seedlings; the two inner disc wall plates can slide in the concentric-square-shaped disc body in the reverse direction to force the seedling growing cavity to expand and extend. The inner disc wall plates and the concentric-square-shaped disc body form the seedling growing cavities, the inner disc wall plates are close in the seedling stage, the temperature control mechanism keeps a distance with roots to prevent the roots from being damaged, the driving set drives the inner disc wall plates to slide reversely to expand the cavities in the seedling stage, the cavities are gradually close to the temperature control mechanism, and the inner disc wall plates are heated due to the fact that the distance is reduced, so that the temperature of the seedling growing cavities is increased step by step, and the temperature requirement of saplings is met; meanwhile, the winding spring and the separation belt are adaptively adjusted along with sliding, so that soil in the soil covering cavity enters the seedling culture cavity through the soil falling holes to supplement the substrate, and the saplings can obtain sufficient space and nutrients in all stages.

The application discloses a hilly and mountainous area seedling planting device and relates to the technical field of forest tree breeding. The device comprises a device body. A through groove is arranged at the middle position of the device body. A handle is fixedly connected to the top surface of the device body. Wheels are fixedly installed at the bottom surface of the device body. A pair of bottom plates are fixedly connected to the bottom surface of the device body. Rods are rotatably connected to the two ends of the pair of bottom plates. Docking seats are fixedly connected to the bottom ends of the rods. The docking seats are fixedly installed on scrapers. Circular rings are fixedly connected to the top surfaces of the docking seats. First springs are arranged on the rods. The device has a reasonable structure. The seedlings can be placed in the device through a feeding assembly. The seedlings can be placed in the earth pits through the cooperation of a transmission assembly and a feeding assembly. The earth pits can be simply covered with soil through the scrapers.

The application discloses a pruning device for garden greening tree breeding, and relates to the technical field of tree breeding pruning, which comprises an arm sleeve and a fixing rod, the fixing rod is installed at the front end of the arm sleeve, small and large cutting pieces are rotationally installed at the bottom of the fixing rod, and a first torsional spring is arranged between the small and large cutting pieces, the first torsional spring resets the small and large cutting pieces, a rear handle is installed at the bottom of the front end of the arm sleeve, and a worker presses the surface of the rear handle with the thumb and the web space part as a force point, so that the worker can easily cut off the branches by holding the device with one hand, compared with the existing pruning tool, the force mode is changed, the worker does not need to frequently bend the arm, wrist fatigue is avoided, and the worker can operate with one hand to release the other hand to adjust the angle of the required cut-off branches, so that the growth angle of the branches is prevented from blocking the small and large cutting pieces from extending to cut off.

The application discloses a kind of intelligent protection device for forest breeding in geological disaster ecological restoration, it is related to the technical field of ecological environment management, including: multidirectional impact base, horizontally set, fixed in soil layer, and forest seedlings are planted in middle part;Rotary connecting device, fixed at the top of multidirectional impact base;Anchor mechanism, annular distribution is equipped with multiple, fixed outside rotary connecting device, corresponding with the vertex of multidirectional impact base;Control adjusting device, fixed at the top of rotary connecting device;Dynamic protection device, fixed on control adjusting device;Auxiliary adjusting device, mobile setting is at the top of dynamic protection device, and fixedly connected with control adjusting device by telescopic arc plate.The application realizes intelligent impact protection under complex terrain and dynamic geological conditions in geological disaster area, and also considers ecological restoration function, significantly improves the protection effect and ecological benefit of forest breeding in geological disaster scene.

The invention discloses a breeding frame convenient to maintain for forest tree breeding, and relates to the technical field of horticultural forest tree breeding, the breeding frame comprises a breeding base, a breeding soil bed, a cleaning structure and a spraying structure, an adjustable soil turning structure is arranged in the middle of the breeding base, and a sowing and discharging structure is arranged on the upper side of the adjustable soil turning structure. Through a soil turning structure driven by double servo motors, horizontal sliding of a soil turning shovel and flexible depth adjustment are achieved by means of transmission of a threaded rod and a gear, and differentiated requirements of different seeds for the soil turning depth are accurately met; according to the seeding structure, a servo motor drives a connecting rod, a sliding sleeve and other components to be linked, the blanking amount and the seeding depth can be accurately controlled, and manual operation errors are reduced; the water pump and the multiple sets of spraying heads cooperate to guarantee uniform supply of water and nutrients, the cleaning structure can efficiently clean residues of the soil turning shovel to avoid cross contamination, the breeding diversity is enhanced through the overall design, the operation efficiency and the equipment reliability are greatly improved, and meanwhile the maintenance cost is effectively reduced.

The invention relates to the technical field of forest tree breeding and seedling raising, in particular to a seedling transplanting device and method for forestry planting, which comprises a steel frame, a mounting plate is slidably arranged in the steel frame, a through hole is formed in the center of the mounting plate, a support ring is rotatably arranged in the through hole, and a support plate rotating along the axis of the support ring is arranged on the outer side of the support ring. The device comprises a supporting ring and a supporting plate, two sets of cutters are fixedly connected to one end of the supporting plate, the supporting plate continuously reciprocates in the rotating process, and a pressing plate is slidably connected to the interior of the supporting ring. The device can effectively reduce falling of soil around seedling roots in the digging or transporting process, prevents soil loss caused by jolting, guarantees survival quality of seedlings in the transporting process, synchronously drives the multiple sets of protruding rods to stamp the upper surface of the soil in the pressing process of the pressing plate, maintains air permeability of the soil in the transporting process, and improves the survival rate of the seedlings. The survival rate after seedling transplantation is improved.

The utility model discloses a forest tree breeding device, and relates to the technical field, the forest tree breeding device comprises a wetting assembly, the wetting assembly comprises a water pipe, a water storage tank, a driving pump and a nozzle, and the driving pump is fixed in the water storage tank; a germination accelerating assembly is further included; the germination accelerating assembly comprises a box body, a storage box, an observation plate, a partition plate, a storage cavity and a breeding cavity; a plurality of breeding cavities are formed in the side wall of the box body, the storage box is slidably connected into the breeding cavities in the box body, the storage box is a rectangular box with an opening in the top, and a gap is reserved between the storage box and the box body; the storage cavities are located in the storage box, the storage cavities are separated by partition plates, and two seeds can be placed in each storage cavity; the partition plate is a rectangular plate; a plurality of rectangular holes are formed in the two opposite side walls of the storage box, and the observation plate is fixed to the rectangular holes and is a transparent plate. The inner side of the observation plate is attached to the seeds; the germination state of the seeds can be observed during germination accelerating, the seeds are transplanted when germinating to a proper state, the ungerminated seeds continue to be subjected to germination accelerating, and tender shoots are prevented from being damaged during transplanting.

The invention relates to the technical field of cultivation for jujube tree breeding, and discloses a cultivation device for jujube tree breeding, which comprises a breeding box, a supporting plate fixedly connected to the surface of the breeding box, supporting legs fixedly connected to the bottom of the breeding box, a seedling bed lifting device arranged in the breeding box, and a ventilation and illumination device arranged on the surface of the breeding box, a circulating stirring device is arranged on the surface of the breeding box, the seedling bed lifting device comprises a limiting block, and the surface of the limiting block is fixedly connected with the inner wall of the breeding box. When a motor is started, an output end drives a threaded rod to rotate together through a rotating rod, when the threaded rod rotates, a lifting leakage plate is driven to drive a soil box to move up and down through extrusion, a round telescopic rod enables the position of the lifting leakage plate to be more stable when the lifting leakage plate moves, and meanwhile when the soil box moves upwards, a breeding plate drives breeding to move upwards together. Therefore, the machine can fully perform ventilation and illumination work on breeding, and the culture stability of the machine is improved.

The invention discloses an application of an antibacterial peptide Hinnavin I in improvement of citrus liberobacter asiaticum resistance, and relates to the technical field of forest breeding, and the application comprises the following steps: introducing a nucleotide sequence for coding the antibacterial peptide Hinnavin I into a citrus plant through a genetic transformation method, and expressing the antibacterial peptide Hinnavin I in the citrus plant to inhibit proliferation of citrus liberobacter asiaticum pathogenic bacteria. According to the invention, the animal-derived antibacterial peptide can be expressed in a transgenic hairy root after a nucleotide sequence for coding Hinnavin I is introduced into a citrus plant; the Hinnavin I inhibits the proliferation of the pathogenic bacteria of the citrus huanglongbing by destroying the membrane structure of the pathogenic bacteria of the citrus huanglongbing, and as the Hinnavin I is an animal-derived peptide, and the expression of the Hinnavin I in plants does not involve chemical pesticides, an environment-friendly and high-targeting green prevention and control means is achieved, and meanwhile, the purpose of cross-species application of animal immune elements in plant disease prevention and control is expanded.

The application relates to a European spruce mature cotyledon embryo phenotype intelligent detection method and system and a storage medium, and relates to the cross technical field of forest tree breeding and computer vision. The detection method in the application first constructs a mobile phone image data set of European spruce somatic embryo differentiation and performs boundary box labeling; secondly, a MultiSeamHead module is introduced based on a YOLOv11n framework, the detection capability for small targets, dense occlusion and complex backgrounds is enhanced through multi-scale feature fusion and a spatial attention mechanism; then, the improved detection model is trained by using the labeled data set; finally, a visual interactive platform is constructed based on PySide6, the trained model is integrated, and the functions of image import, automatic detection, result visualization and data export are realized. Experiments show that the precision of the improved model reaches 0.861, the recall rate reaches 0.863, and the mAP@50 reaches 0.927, which is significantly improved compared with the original model, efficient, accurate and high-throughput mature cotyledon embryo phenotype automatic detection is realized, and the technical application threshold is reduced.

The present application relates to the technical field of rubber tree breeding, and particularly relates to a method for promoting rubber tree somatic embryo secondary somatic embryogenesis and plant regeneration and application thereof. The present application provides application of magnesium hydride in promoting rubber tree somatic embryo callus induction, secondary somatic embryogenesis or plant regeneration. Based on the research results, magnesium hydride exhibits a significant regulation effect in the process of rubber tree somatic embryogenesis, development and plant regeneration. Through treatment of magnesium hydride with a suitable concentration, the induction efficiency can be effectively improved, and the growth state of callus is optimized, so that the callus presents a high-quality morphology with loose structure and fresh color. In terms of plant regeneration, magnesium hydride treatment significantly improves the germination rate, promotes the optimization of growth indexes such as plant height, stem diameter, root length and leaf number of regenerated plants, and improves the construction quality and growth potential of plants. In summary, magnesium hydride provides a new technical approach for the establishment of efficient and stable somatic embryogenesis system and plant regeneration of rubber tree.