The invention relates to a formula of health-preserving rhizome gastrodiae wine for middle-aged and aged people. The formula is characterized by comprising, by mass, 38 parts of rhizome gastrodiae, 5 parts of American ginseng, 15 parts of rock sugar, 3 parts of aspergillus oryzae, 5 parts of green plums, 8 parts of longan pulp, 10 parts of angelica, 8 parts of semen cannabis, 9 parts of rehmannia glutinosa, 15 parts of radix solanum melongena, 6 parts of fruits of Chinese wolfberry, 6 parts of eucommia ulmoides, 5 parts of brewer's yeast, 9 parts of rhizoma polygonati, 50-150 parts of distilled spirit, 20-30 parts of bee milk, 15 parts of rose pollen, 15 parts of hawthorn fruits, 30 parts of robinia flower pollen, 10 parts of mulberry fruits, 10 parts of jujube fruits, 8 parts of processed radix aconiti lateralis, 15 parts of paeoniae radix rubra, and 20-30 parts of schisandra chinensis. According to the formula and the preparation, the health-preserving rhizome gastrodiae wine resistant to fatigue and capable of nourishing yin and yang and enhancing immunity of a body is obtained, original flavor of the rhizome gastrodiae is kept to the utmost, the flavor of existing rhizome gastrodiae wine is improved, the aroma degree of the rhizome gastrodiae wine is increased, consumer's drinking needs are satisfied, and the consumer market is expanded.