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40 results about "RAPE HONEY" patented technology

Identification method for rape honey doped in acacia honey

The invention discloses an identification method for rape honey doped in acacia honey. Firstly, chemical compounds of phenolic acids are extracted from honey by means of resin, and chromatographically pure methyl alcohol of a metered volume is utilized to prepare a test solution; then certain amounts of ten kinds of phenolic acid comparison products are respectively taken, and chromatographically pure methyl alcohol of a metered volume is utilized to prepare a comparison product solution. The determination method comprises the steps that the comparison product solution and the test solution are respectively absorbed to be injected into a high performance liquid chromatograph, a chromatogram is measured and recorded by making the comparison products as reference substances, an HPLC-ECD fingerprint spectrum of the acacia honey is obtained, an HPLC-ECD fingerprint spectrum of the rape honey and an HPLC-ECD fingerprint spectrum of the acacia honey containing the rape honey are obtained by means of the same method, the three spectrums are compared, and by combining a chemometrics method, whether the rape honey is doped in the acacia honey is identified. The identification method is reasonable in design, good in separation effect and high in sensitivity and feasibility, and resolves an important technical problem in acacia honey adulteration identification and quality control.
Owner:西安众天食品有限责任公司

Method for identifying rape honey and vervain family honey

The invention discloses a method for identifying rape honey and vervain family honey. The method is characterized by comprising the following steps of 1, constructing an identification model by treating provided samples of rape honey and vervain family honey, determining content of amino acids obtained by honey protein hydrolysis, wherein the number of each kind of the samples is greater than or equal to 15, setting Y variables as classified variables, setting values of the Y variables of rape honey and vervain family honey respectively as 0 and 1, setting a threshold value as 0.5, carrying out linear regression of the determined amino acid content and the classified variables of the samples by a partial least square discriminant analysis method, and building the identification model, and 2, carrying out identification by treating honey needing to be identified, determining content of amino acids obtained by honey protein hydrolysis, carrying out identification by the identification model obtained by the step 1 according to the determined amino acid content, and determining if the identified honey belongs to rape honey or vervain family honey. The method can effectively identify rape honey and vervain family honey.
Owner:BEE RES INST CHINESE ACAD OF AGRI SCI

All-natural bee pollen facial mask paste and production method thereof

The invention discloses an all-natural bee pollen facial mask paste and a production method thereof. According to weight percentage, the component raw materials include: 33-38% of wall-breaking rape pollen, 50-55% of rape honey, 7-9% of fresh royal jelly and 4-6% of pearl powder. According to the all-natural bee pollen facial mask paste and the production method thereof, effects of the normal facial mask products of separating air, infiltrating and softening cuticles, promoting skin blood circulation, tightening skins, removing surplus grease and ageing cutins are kept, facial alimentation absorption is enhanced, natural elasticity and gloss of skins are recovered, more importantly, side effects of the normal facial mask products cannot be generated, and the all-natural bee pollen facial mask paste has good protection effect for faces.
Owner:ANHUI WANGCHAO FOOD

Method for identifying linden honey, vicia villosa Roth honey and rape honey

The invention discloses a method for effectively identifying linden honey, vicia villosa Roth honey and rope honey. The method comprises the following steps: firstly extracting a phenolic compound from honey samples by using XAD-2 type resin, carrying out HPLC-ECD (High Performance Liquid Chromatography-Electrochemical Detector) analysis, establishing HPLC-ECD fingerprint spectrums of the linden honey sample, the vicia villosa Roth honey sample and the rape honey sample by using obtained HPLC-ECD spectrum data, and analyzing the fingerprint spectrums of the linden honey sample, the vicia villosa Roth honey sample and the rape honey sample to obtain a large amount of common peak information; carrying out analysis and comparison on the common peak information, then picking out 17 common peaks in total as characteristic common peaks, and classifying the three kinds of honeys by combining a chemometrics method. The method disclosed by the invention is reasonable in design; selected analysis instruments are high in sensitivity, good in separation effect and high in feasibility; an important technical problem in quality control of the linden honey and the vicia villosa Roth honey is solved.
Owner:NORTHWEST UNIV(CN)

Method for detecting rape honey aroma components by solid phase microextraction

The invention relates to a method for detecting rape honey aroma components by solid phase microextraction, and the method comprises the following steps: weighing 6 mL of rape honey, adding 400 mg of sodium chloride, aging an extraction head at an introduction port of a gas chromatograph at an aging temperature of 250 DEG C for 2 hours, inserting the aged extraction head into a headspace part of a sample bottle, pushing a fiber head out, carrying out constant temperature magnetic stirring adsorption at 60 DEG C for 50 min, inserting the extraction head into a GC-MS introduction port, carrying out desorption at 250 DEG C for 5 min, and then carrying out gas chromatography-mass spectrometry. The method of the invention can rapidly detect aroma components in rape honey, and can identify whether the rape honey is adulterated by comparison with standard rape honey aroma substances.
Owner:CHINA NAT INST OF STANDARDIZATION

Identification method of chaste honey, rape honey and acacia honey based on NMR (Nuclear Magnetic Resonance) technique

The invention discloses an identification method of chaste honey, rape honey and acacia honey based on an NMR (Nuclear Magnetic Resonance) technique. The identification method comprises the followingsteps: filtering a honey sample and then preparing the filtered honey sample into 0.03 to 0.5 g / L solution with heavy water; adding a phosphate buffered solution with the concentration of 0.5 to 2.0 mol / L, wherein the phosphate buffered solution contains a 3-trimethylsilyl-1-sodium propyl sulfonate internal standard solution with the volume fraction of 0.05 to 0.3 percent and 0.1 to 3.0 mM of sodium azide; mixing, adjusting the pH value of the phosphate buffered solution to 2.5 to 4.0 and measuring by a NMR spectrometer to obtain an NMR spectrum; and analyzing chemical shift of a honey samplemarker, and determining that the honey sample of which 1H chemical shift delta is 7.0 to 7.5 ppm and a deviation delta is within a range of 0.02 to 0.05 ppm is the chaste honey, otherwise the honey sample is the rape honey or the acacia honey. The method has the advantages that a large quantity of samples can be analyzed in a short time and the chaste honey, the rape honey and the acacia honey canbe quickly identified.
Owner:ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU +1

Extraction method of intelligent olfaction spectrum feature representing differences of honey based on independent component analysis

An extraction method of intelligent olfaction spectrum feature representing differences of honey based on an independent component analysis is characterized in that: the main component analysis is classified according to the maximization of variance between data, namely the secondary moment of data, and the independence of data in the high order moment is neglected; the independent component carries out transformation of matrix by utilizing the high order moment between data, thus related rows between feature vectors are further decreased, and the compressing effect of signals is strengthened; the discrimination rate is 94.94%, when the independent component is 14, the honey sample is 75 / 79, wherein the rape honey is 22 / 23, linden honey is 16 / 17, and acacia honey is 37 / 39.
Owner:CHINA NAT INST OF STANDARDIZATION

Honey detection method based on support vector machine algorithm optimization

The invention relates to a honey detection method based on support vector machine algorithm optimization. The honey detection method comprises the following steps: selecting 5 different honey sources including oilseed rape honey extracted from Fuling district and Yongchuan district of Chongqing of the western China, honey of lychee flowers extracted from Nanning of Guangxi of the south China, chaste honey extracted from Miyun of Beijing of the north China, acacia honey extracted from Laiyang of Shandong and basswood honey extracted from Dunhua of Jilin and Harbin of Heilongjiang of northeast as study samples; detecting the to-be-detected sample honey by utilizing adsorption difference of a gas sensor array with different volatile components; changing surface layer current intensity of a semiconductor sensor after the volatile components of the honey are adsorbed with sensor features, and obtaining response curves of each sample through digital conversion so as to detect and analyze the samples and establish a support vector machine discrimination model by utilizing extracted electronic nose feature information to classify the samples of different honey sources.
Owner:CHINA NAT INST OF STANDARDIZATION

Technology for producing greasy crystallized honey

ActiveCN102894247AMeet special high-end needsAvoid accidentFood preparationWater bathsWide mouth
The invention discloses a technology for producing greasy crystallized honey. The technology comprises the following steps of 1, a seed preparation comprising mixing rape honey, clean water and micro-grinded bee pollen, stirring to obtain a seed mixed solution, heating rape seed raw material honey in a water-bath, adding the heated rape seed raw material honey into the seed mixed solution with stirring, filtering by a sieve of 60 meshes, pouring the filtrate into a wide-mouth container, and putting the wide-mouth container into an inclosed thermostatic chamber having a temperature of 15 to 20 DEG C for 10 to 15 days to obtain crystal seeds, 2, a raw material honey selection process comprising selecting all natural honey having a light color, low water content less than 20% and high glucose content more than 35% as a raw material for production of the greasy crystallized honey, and 3, a raw material honey inoculation and crystallization process comprising a, inoculation, b, pre-crystallization, and c, after-crystallization. The greasy crystallized honey obtained by the technology has white and smooth appearance, a fine taste, and good stability, plasticity and repeatability.
Owner:XINJIANG TIANSHAN BLACK BEE IND PARK

Method for identifying mature rape honey

The invention provides a method for identifying mature rape honey. The method comprises the following steps that: to-be-detected honey is detected by adopting liquid chromatography-quadrupole rod-time-of-flight mass spectrometry; and excimer ion peaks with the mass number of 207.07 + / -0.01 are adopted as characteristic peaks, when it is judged that the to-be-detected honey is mature rape honey when the time-of-flight mass spectrometry contains the characteristic peaks. According to the method, it is determined that the excimer ion peaks with the mass number of 207.07 + / -0.01 can serve as the characteristic peaks for identifying the mature rape honey, only the mature rape honey contains the characteristic peaks, and non-mature rape honey does not contain the characteristic peaks. The methodfor identifying the mature rape honey is quick and accurate, has important significance for identifying the mature rape honey, and provides a guarantee for controlling the quality of the honey and maintaining the market order.
Owner:BEE RES INST CHINESE ACAD OF AGRI SCI

Method for identifying mature and concentrated rape honey, acacia honey and vitex negundo honey by using pyruvic acid

The invention provides a method for identifying mature and concentrated rape honey, acacia honey and vitex negundo honey by using pyruvic acid, and belongs to the technical field of food detection. The method comprises the steps of respectively dissolving a proper amount of pyruvic acid and various mature and thermally concentrated honey in a phosphate buffer solution, adjusting the pH value to 3.0+ / -0.1, and obtaining hydrogen spectrum data of pyruvic acid and various honey by adopting a 400MHz nuclear magnetic resonance spectrometer with a BBI probe. The hydrogen spectrum data of pyruvic acid shows that the characteristic peak of pyruvic acid is a single peak near the displacement of 2.37+ / -0.02ppm, for example, the hydrogen spectrums of honey samples of rape honey, acacia honey and vitex negundo honey have a single peak at the same position and are higher than the peripheral peaks, wherein if the hydrogen spectrum is higher than the peripheral peaks, the honey is concentrated honey,otherwise, the honey is mature honey. The method provided by the invention is simple and efficient, and can be used for accurately identifying mature and thermally concentrated rape honey, acacia honey and vitex negundo honey.
Owner:JIANGSU SINOGRAPHY TESTING

Honey detection composition, kit, detection method and application

InactiveCN103215373ADetection of initial copy numberReal-time monitoring of PCR reaction processMicrobiological testing/measurementLocustOligonucleotide Primer
The invention relates to an oligonucleotide primer and a probe for detecting the longan, lychee, locust and rape honey plant components in honey, a kit comprising the primer and the probe, and a method for detecting the honey plant components in honey. According to the invention, the honey plant components in honey can be simply, quickly, specifically and sensitively detected.
Owner:CHINESE ACAD OF INSPECTION & QUARANTINE

Method for identifying mature rape honey

The invention provides a method for identifying mature rape honey. The method comprises the following steps that: to-be-detected honey is detected by adopting liquid chromatography-quadrupole rod-time-of-flight mass spectrometry; excimer ion peaks with retention time of 8.99 + / -0.10 min and the mass number of 285.04 + / -0.01 as characteristic peaks, and when it is judged that the to-be-detected honey is mature rape honey when a detection spectrum contains the characteristic peaks. The liquid chromatography detection conditions are as follows: a C18 chromatographic column is adopted; a mobile phase A is 0.1% formic acid aqueous solution; a mobile phase B is methanol; the volume fraction of the mobile phase B is increased to 5% within 0-1 min of gradient elution; the volume fraction of the mobile phase B is increased from 5% to 55% within 1-6 min of gradient elution; the volume fraction of the mobile phase B is increased from 55% to 95% within 6-20 min of gradient elution; the volume fraction of the mobile phase B is maintained at 95% within 20-26 min of gradient elution; and the volume fraction of the mobile phase B is reduced to 5% within 26-27 min of gradient elution. The method provided by the invention can be used for identifying the mature rape honey quickly and accurately.
Owner:BEE RES INST CHINESE ACAD OF AGRI SCI

Mixed granulated honey and preparation method thereof

The invention relates to the technical field of foods and provides mixed granulated honey and a preparation method thereof. The mixed granulated honey specifically comprises following components, by weight component, that 10-20 crystallization revulsant and 78-92 liquid honey, wherein the liquid honey, by weight component, comprises 14-16 acacia honey, 50-60 rape honey and 14-16 sophora alopecuroide honey; and the crystallization revulsant is one or more than one kind of single granulated honey. The mixed granulated honey has the advantages of low temperature in granulating process, fast speed, uniform crystallization, and easiness in honey conservation and original freshness maintenance. And moreover, the nutrition of the honey is lossless, and the taste and flavor of the single granulated honey are enriched.
Owner:湖南明园蜂业科技有限公司

Method for identifying litchi honey

The invention discloses a method for identifying litchi honey. The method comprises the following steps: firstly, constructing an HPLC-DAD (High Performance Liquid Chromatography-Diode Array Detection) fingerprint chromatogram of litchi honey, chaste honey and rape honey so as to determine characteristic markers of the litchi honey; then, separating and purifying the determined characteristic markers according to a preparative liquid chromatography so as to respectively obtain two kinds of characteristic markers with higher purity; and finally, analyzing the molecule information of the characteristic markers by using a quadrupole / time-of-flight mass spectrometer so as to finally determine that the molecular weight of the two kinds of characteristic markers is 264 and the molecular formulais C15H20O4, thereby conjecturing that the two kinds of characteristic markers are isomers. The method disclosed by the invention is reasonable in design, and the selected analytical instrument is high in sensitivity, high in analysis speed, high in separation efficiency and highstrong in feasibility and provides a basis for authenticity identification and quality control of the litchi honey.
Owner:NORTHWEST UNIV(CN) +1

Honey not easy to crystallize and making process thereof

The invention provides honey not easy to crystallize. The honey is characterized by being made from, by weight, 10-20% of robinia honey, 20-40% of thorn honey and 30-60% of rape honey. The invention further provides a preparation method of the honey not easy to crystallize. The method includes the following steps of firstly, melting honey; secondly, stirring the mixed honey, wherein three types of melted honey including 10-20% of robinia honey, 20-40% of thorn honey and 30-60% of rape honey are evenly stirred according to the weight percentages; thirdly, conducting rough filtering, intermediate filtering and fine filtering sequentially; fourthly, conducting vacuum dewatering and concentrating. Three types of raw honey in different proportions are mixed to form the honey, the honey not easy to crystallize is obtained by strictly controlling the technological parameters of all procedures in the honey production technological process on the premise of not adding any additives, and the honey has the advantages of being attractive in appearance, capable of being absorbed by human bodies, good in taste and low in price.
Owner:SUZHOU SHANDING BEE PROD CO LTD

Method of identifying whether rape honey is mixed in bee honey

ActiveCN109490432ASensitiveUniversal identificationComponent separationBees honeyRAPE HONEY
The invention provides a method of identifying whether rape honey is mixed in bee honey and belongs to the technical field of food detection. The method includes detecting the content of methyl syringate in single-flower bee honey under identification; if the content is greater than 10 mu g / 100 g, indicating that rape honey is mixed in the single-flower bee honey under identification; if the content if less than 10 mu g / 100 g, indicating that no rape honey is mixed in the single-flower bee honey under identification. The method has the advantages that detection results are reliable and stable,judgment degree is high, operation is convenient, and the cost is low.
Owner:新疆新兴源农业有限公司

All-natural bee pollen facial mask paste and production method thereof

The invention discloses an all-natural bee pollen facial mask paste and a production method thereof. According to weight percentage, the component raw materials include: 33-38% of wall-breaking rape pollen, 50-55% of rape honey, 7-9% of fresh royal jelly and 4-6% of pearl powder. According to the all-natural bee pollen facial mask paste and the production method thereof, effects of the normal facial mask products of separating air, infiltrating and softening cuticles, promoting skin blood circulation, tightening skins, removing surplus grease and ageing cutins are kept, facial alimentation absorption is enhanced, natural elasticity and gloss of skins are recovered, more importantly, side effects of the normal facial mask products cannot be generated, and the all-natural bee pollen facial mask paste has good protection effect for faces.
Owner:ANHUI WANGCHAO FOOD

Matcha-custard-puerariae radix starch freezing product production method

InactiveCN106560087AFood ingredient functionsPuerariae radixXanthan gum
The invention discloses a matcha-custard-puerariae radix starch freezing product production method, wherein the raw materials comprise fresh and wild puerariae radix, edible matcha, rape honey, condensed milk, nata de coco, xanthan gum, custard powder, sodium dehydroacetate, and purified water. The preparation steps of the invention comprise puerariae radix starch preparing, processing molding, and vacuum packaging.
Owner:TIANCHANG HIGH TECH ENTREPRENEURSHIP SERVICE CENT

Rape honey authenticity recognition method based on principal component analysis and gradient boosting decision tree

The invention discloses a rape honey authenticity recognition method based on principal component analysis and a gradient boosting decision tree. Comprising the steps of collecting and preparing true and false honey samples and generating honey data, performing true and false labeling on honey data records to obtain a rape honey data set, performing dimensionality reduction on the rape honey data set by using a principal component analysis (PCA), constructing a rape honey true and false recognition model by using a gradient boosting decision tree (GBDT) algorithm, and evaluating the effectiveness of the model. And carrying out authenticity recognition on to-be-detected honey by using the rape honey authenticity recognition model. According to the method, the root-mean-square error and the AUC value are effectively improved, the data feature dimension, the model training time and the model complexity are reduced, the authenticity of the rape honey can be effectively and accurately recognized, the error caused by manually checking the spectrogram for authenticity recognition is avoided, and the accuracy of the authenticity recognition of the rape honey is improved.
Owner:CHINESE ACAD OF INSPECTION & QUARANTINE +1

Halal sesame oil cake and preparation method thereof

The invention discloses a halal sesame oil cake and a preparation method thereof. The halal sesame oil cake is prepared from the following raw materials in parts by weight: 28-45 parts of flour, 20-28 parts of white granulated sugar, 30-40 parts of eggs, 0.1-0.5 part of honey, 0.1-0.5 part of cake oil 0.2-0.8 part of baking powder, 5-10 parts of sesame oil, 2-5 parts of salad oil and 10-15 parts of fresh milk, and is prepared by the steps of feeding, mixing, molding and baking. According to the conditions that the thermal stability of bioactive substances of enzyme, vitamins and the like in rape honey is relatively poor, the nutrient value of the rape honey is easily reduced, and the rape honey is not suitable for being cooked at a high temperature, so that the nutrient ingredients of the rape honey in the sesame oil cake are ensured through short-term baking of the surface, a mixed liquid of the sesame oil and the rape honey is brushed in the cooling process after baking is completed, and the nutrient ingredients are added while the taste is ensured.
Owner:安庆市柏兆记食品有限公司

Detection method of trigonelline and application of trigonelline in honey identification

The invention relates to a detection method of trigonelline and application of the trigonelline in honey identification, and the detection method of the trigonelline comprises the following steps: (1) dissolving a sample with an aqueous solution, and diluting with acetonitrile; (2) detecting the trigonelline in the honey sample by adopting a liquid chromatography-tandem mass spectrometer; furthermore, the rape honey can be effectively identified according to the content of the trigonelline in the honey.
Owner:秦皇岛海关技术中心

Royal jelly product preparation and storage method

The invention relates to a royal jelly product preparation and storage method. The royal jelly product preparation method comprises the following steps of (1) royal jelly producing frame production, wherein each royal jelly producing frame is made of a plurality of division bars, and one stylobate is produced every 1-2 cm on each division bar, wherein 60-80 stylobates are arranged on each royal jelly producing frame; (2) larva transplantation, wherein each stylobate is internally coated with a layer of royal jelly, bee larvae are transplanted into the stylobates, and 4-5 royal jelly producing frames are placed into each beehive side by side; (3) royal jelly preparation, wherein bees in the beehives secret beewax to heighten the stylobates so that queen cells can be formed while pouring royal jelly into the stylobates, and after 60-72 h, the royal jelly producing frames are taken out of the beehives; (4) royal jelly collecting, wherein when the royal jelly accounts for 3 / 4-4 / 5 of the size of each queen cell, the bee larvae are moved out or not, and the queen cells are detached from the royal jelly producing frames to obtain royal jelly products. Ripe honey is poured into the queen cells of the royal jelly products, and the queen cells are sealed; the sealed queen cells are soaked into rape honey for storage. The royal jelly products have the advantages of being high in activity and convenient to store.
Owner:北京天宝康高新技术开发有限公司

Method for detecting rape honey aroma components by solid phase microextraction

The invention relates to a method for detecting rape honey aroma components by solid phase microextraction, and the method comprises the following steps: weighing 6 mL of rape honey, adding 400 mg of sodium chloride, aging an extraction head at an introduction port of a gas chromatograph at an aging temperature of 250 DEG C for 2 hours, inserting the aged extraction head into a headspace part of a sample bottle, pushing a fiber head out, carrying out constant temperature magnetic stirring adsorption at 60 DEG C for 50 min, inserting the extraction head into a GC-MS introduction port, carrying out desorption at 250 DEG C for 5 min, and then carrying out gas chromatography-mass spectrometry. The method of the invention can rapidly detect aroma components in rape honey, and can identify whether the rape honey is adulterated by comparison with standard rape honey aroma substances.
Owner:CHINA NAT INST OF STANDARDIZATION

A Feature Extraction Method of Intelligent Olfactory Atlas Based on Single Item Discriminant Method to Represent the Difference of Honey

A feature extraction method based on the single-item discriminant method to characterize the difference of honey in the intelligent olfactory spectrum, which is characterized in that all feature points are pattern-recognized one by one, and the difference in discrimination accuracy is compared when each signal point is used as a single feature. The recognition method is embedded in the feature selection. By combining the discriminant method, the ability of each signal point to predict the sample can be obtained. This method is more dependent on the selected pattern recognition method, and the selection result will change with the discriminant method. Single vector selection The difference in the accuracy rate among different sensors is small, while the difference between the information points at different detection times in the same sensor is relatively large, but the change trend of the Bayesian discrimination accuracy rate at different time points in different sensors is consistent with the change trend of the variance ratio, that is, the detection The initial signal discrimination accuracy rate is relatively high, concentrated in the first 30s detection time of each sensor, but the effect in the later detection period is poor, and the signal points with a discrimination accuracy rate greater than 60% are selected as feature points, a total of 598 feature points, of which rapeseed honey 20 / 23, linden honey 13 / 17, acacia honey 34 / 39.
Owner:CHINA NAT INST OF STANDARDIZATION

A honey detection method based on parameter selection of support vector machine classifier based on particle swarm optimization

A honey detection method based on particle swarm optimization and support vector machine classifier parameter selection, which is characterized in that random individuals are initialized, and individual changes are performed by calculating the gap between the current individual fitness function value and the optimal fitness value of the group. Compared with genetic Algorithm, the particle swarm optimization algorithm converges faster, and reaches the optimal point in about 6 generations. The optimization result is: the highest accuracy rate of the training set is 91.25%, c=32.3362, r=0.0100. Under this condition, the prediction accuracy rate is 88.61%, Among them, rapeseed honey is 21 / 23, linden honey is 14 / 17, and acacia honey is 36 / 39.
Owner:CHINA NAT INST OF STANDARDIZATION

An identification method based on nuclear magnetic resonance technology for vitex honey, rapeseed honey and acacia honey

The invention discloses an identification method of chaste honey, rape honey and acacia honey based on an NMR (Nuclear Magnetic Resonance) technique. The identification method comprises the followingsteps: filtering a honey sample and then preparing the filtered honey sample into 0.03 to 0.5 g / L solution with heavy water; adding a phosphate buffered solution with the concentration of 0.5 to 2.0 mol / L, wherein the phosphate buffered solution contains a 3-trimethylsilyl-1-sodium propyl sulfonate internal standard solution with the volume fraction of 0.05 to 0.3 percent and 0.1 to 3.0 mM of sodium azide; mixing, adjusting the pH value of the phosphate buffered solution to 2.5 to 4.0 and measuring by a NMR spectrometer to obtain an NMR spectrum; and analyzing chemical shift of a honey samplemarker, and determining that the honey sample of which 1H chemical shift delta is 7.0 to 7.5 ppm and a deviation delta is within a range of 0.02 to 0.05 ppm is the chaste honey, otherwise the honey sample is the rape honey or the acacia honey. The method has the advantages that a large quantity of samples can be analyzed in a short time and the chaste honey, the rape honey and the acacia honey canbe quickly identified.
Owner:ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU +1
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