Method for identifying rape honey and vervain family honey

A technology of chattia honey and rapeseed honey, which is applied in the field of identification of rapeseed honey and chattia honey, can solve the problems of complex operation, high dependence on identification result model establishment, and high identification cost

Inactive Publication Date: 2013-09-04
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Application Information

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Problems solved by technology

This method requires the use of equipment such as Fourier transform near-infrared spectrometers, which has high identification costs and complicated operations. At the same time, the identification results are highly dependent on model establishment.

Method used

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  • Method for identifying rape honey and vervain family honey
  • Method for identifying rape honey and vervain family honey
  • Method for identifying rape honey and vervain family honey

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Embodiment 1

[0028] Embodiment one: the identification method of rapeseed honey and twig honey, comprises the following steps:

[0029] 1) Extraction and hydrolysis of honey protein: Accurately weigh 10.0g (accurate to 0.01g) of honey sample into a 50mL centrifuge tube, add 20mL of water, mix well, and centrifuge at 1500g at 4°C for 10min to remove the pollen grains at the bottom. The supernatant was transferred to another centrifuge tube. Add 4 mL of 100% trichloroacetic acid to the supernatant, mix thoroughly, and place at -20°C for 15 minutes, then place at 4°C for 1 hour to precipitate the protein in honey. Centrifuge the centrifuge tube at 4°C, 10000g for 10min, discard the supernatant, and transfer the protein precipitate at the bottom layer to the hydrolysis tube with 3mL, 3mL and 4mL of 6.0mol / L hydrochloric acid solution three times, and fill it with nitrogen Finally, use a vacuum pump to vacuum, seal the hydrolysis tube and hydrolyze it in a constant temperature drying oven at 1...

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Abstract

The invention discloses a method for identifying rape honey and vervain family honey. The method is characterized by comprising the following steps of 1, constructing an identification model by treating provided samples of rape honey and vervain family honey, determining content of amino acids obtained by honey protein hydrolysis, wherein the number of each kind of the samples is greater than or equal to 15, setting Y variables as classified variables, setting values of the Y variables of rape honey and vervain family honey respectively as 0 and 1, setting a threshold value as 0.5, carrying out linear regression of the determined amino acid content and the classified variables of the samples by a partial least square discriminant analysis method, and building the identification model, and 2, carrying out identification by treating honey needing to be identified, determining content of amino acids obtained by honey protein hydrolysis, carrying out identification by the identification model obtained by the step 1 according to the determined amino acid content, and determining if the identified honey belongs to rape honey or vervain family honey. The method can effectively identify rape honey and vervain family honey.

Description

technical field [0001] The invention relates to a method for identifying honey, in particular to a method for identifying rapeseed honey and twig honey. Background technique [0002] As a natural health care product and nutritional product, honey is favored by consumers. However, with the continuous improvement of people's awareness of product quality and safety, manufacturers are required to indicate the source of honey, especially the plant source, that is, the nectar source plant. name. Honey from different nectar plants has obvious differences in taste, smell, color and nutritional components. Due to the trend of interests, some unscrupulous manufacturers will shoddy and confuse real ones. Therefore, it is necessary to develop effective and reliable methods to identify honey plants. source type. [0003] Although there are some differences in the color and smell of honey from different nectar-source plants, it is difficult to distinguish and identify them through these...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 张金振陈兰珍李熠吴黎明赵静
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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