172 results about "Amino acid content" patented technology

The invention discloses saccharomyces cerevisiae FNFHSc18 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain FNFHSc18 for synthesizing immune nutrients-beta-carotene and astaxanthin, and the preservation number of the saccharomyces cerevisiae engineering strain is CGMCC (China General Microbiological Culture Collection Center) No.24838; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid state fermentation substrate, the content of crude protein and amino acid of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, more importantly, the fermented soybean meal is rich in beta-carotene and astaxanthin, and the content of the beta-carotene and the astaxanthin is greatly reduced. The soybean meal protein obtains new functional immune nutrition, so that the overall nutritional value of the soybean meal is improved, and the soybean meal reaches a leading level in the field of developing functional fermented soybean meal by utilizing saccharomyces cerevisiae.

The invention discloses a preparation process of high-efficiency absorption fish protein peptide and application of the high-efficiency absorption fish protein peptide in fertilizer, and belongs to the technical field of marine organisms. The aquatic product protein is directly used as a fermentation raw material, and the content of amino acid in the fish protein biological peptide is remarkably increased by utilizing the synergistic fermentation effect of microorganisms and assisted by biological enzymolysis, so that the absorptivity of the fish protein peptide is increased; the prepared fish protein peptide is used in the fertilizer to prepare the fish protein peptide functional fertilizer, the nutritional value and the utilization rate of the fertilizer are remarkably improved, and the fish protein peptide functional fertilizer has the remarkable effects of growth promotion, disease resistance, stress resistance and the like. According to the method, a conventional fish protein peptide preparation method of firstly preparing fermentation enzyme liquid and then performing biological enzymolysis is abandoned, the efficient absorption fish protein peptide is prepared by adopting a process of directly fermenting aquatic protein, a fermentation process and an enzymolysis process are integrated, and compared with a conventional process, the method has the advantages of simple production process, low production cost and the like; the method has significant effects and meanings for promoting green preparation of the fish protein peptide and efficient application of the fish protein peptide in fertilizers.

The invention provides a branched chain amino acid transaminase gene and application thereof, and belongs to the field of gene engineering. According to the invention, the branched chain amino acid transaminase gene HvBCAT5 is found for the first time, and the gene has obvious positive correlation with the accumulation of valine (Val), isoleucine (Ile) and leucine (Leu) of crops. The invention provides an important target gene HvBCAT5 for crop quality improvement, and has important value for increasing the content of branched chain amino acid of crops and improving dietary nutrition. The HvBCAT5 gene as well as a recombinant vector, a recombinant bacterium and a transgenic plant thereof have good application prospects in the fields of food and feed industry.

The invention relates to a method for detecting the content of amino acid in a pesticide through pre-column derivatization liquid chromatography, and relates to the technical field of chemical analys.The method comprises the steps that a pesticide sample to be detected is extracted, and a solution containing amino acid is obtained; then, under the optimized alkaline pH and heating conditions, 4-fluoro-7-nitro-2, 1, 3-benzoxadiazole (NBD-F) is adopted as a derivatization reagent to be subjected to a pre-column derivatization reaction with amino acid, and a stable high-fluorescence NBD-F derivative is formed; injecting the derivative liquid into a high performance liquid chromatography system provided with a phenyl bonded phase chromatographic column, and efficiently separating the NBD-F derivative by utilizing pi-pi interaction and hydrophobic interaction of the phenyl column; and finally, detecting and quantifying through a fluorescence detector. Through the synergistic effect of NBD-F derivation and the phenyl column, the separation selectivity and the detection sensitivity are remarkably improved, and the reaction efficiency and the derivative stability are guaranteed through optimized derivation conditions.

The invention belongs to the field of fertilizers, and particularly relates to a foliar fertilizer with high amino acid synthesis efficiency and a preparation method thereof.The preparation method comprises the steps that spermidine is taken to be subjected to formic acid esterification modification, and formic acid esterification spermidine is obtained; the preparation method comprises the following steps: hydrolyzing 2, 4-epibrassinolide to obtain carboxylated brassinolide; under the action of Tween, carboxylated brassinolide and formic acid esterified spermidine are combined to form a fertilizer base full of emulsified liquid drops; and adding other nutrients to obtain the foliar fertilizer. The leaf fertilizer for increasing the amino acid content of rice can increase the content of amino acids, especially lysine, in grains on the premise of increasing the yield of rice, and nitrogen absorption and utilization of the rice grains are improved.

The invention discloses an ecological planting method for remarkably improving the amino acid content of citrus, which comprises the following steps: (1) mixing and fermenting pruned citrus branches, weed straws, livestock and poultry manure and indigenous probiotic ferment powder to prepare a basic microbial fertilizer, and adding a calcium magnesium phosphate fertilizer and monopotassium phosphate to prepare a special microbial fertilizer for citrus, applying as a base fertilizer in winter; (2) after citrus pruning, in key phenological periods such as a flower bud period, a young fruit period and a fruit swelling period, an indigenous probiotic enzyme diluent with a specific dilution ratio is used for systematic foliage spraying on a tree body. The indigenous probiotic enzyme contains bacillus amyloliquefaciens, saccharomycetes, active enzymes and a plurality of nutrient elements. The method can significantly increase the total amino acid content of citrus fruits, greatly increase the yield, improve the taste of the fruits, enhance the tree body resistance, reduce the use of chemical pesticides, improve soil, and realize quality improvement, yield increase and green sustainable development of citrus planting.

The invention discloses an amino acid water-soluble fertilizer and a production process thereof, and relates to the technical field of water-soluble fertilizers. Free amino acid is used as an organic nitrogen source, and the medium element calcium is combined, so that the crop quality is improved. The formula is low in cost, high in absorptivity, suitable for crop growth, simple and convenient to use and high in cost performance, and promotes the synergistic effect of nutrient elements. The method takes amino acid and calcium as raw materials, and has the characteristics of wide applicability, high output-input ratio and easiness in market promotion. The product is completely water-soluble, environment-friendly and safe, can regulate the physiological function of crops, increase the yield and amino acid content and improve the quality, and has remarkable economic benefits. In addition, activated ricinoleic acid carboxyl is combined with fucoidan enzymatic hydrolysis oligosaccharide to construct the microcapsule, so that efficient utilization of resources is realized.

The invention provides a branched chain amino acid transaminase gene and application thereof, and belongs to the field of gene engineering. According to the invention, the branched chain amino acid transaminase gene HvBCAT3 is found for the first time, and the gene is obviously positively correlated with the accumulation of leucine (Leu) of crops. The invention provides an important target gene HvBCAT3 for crop quality improvement, and has important value for increasing the content of branched chain amino acids of crops and improving dietary nutrition. The HvBCAT3 gene as well as a recombinant vector, recombinant bacteria and transgenic plants thereof have good application prospects in the fields of food and feed industry.

The invention provides a branched chain amino acid transaminase gene and application thereof, and belongs to the field of gene engineering. According to the invention, the branched chain amino acid transaminase gene HvBCAT1 is found for the first time, and the gene has obvious positive correlation with valine (Val) and isoleucine (Ile) accumulation of crops. The invention provides an important target gene HvBCAT1 for crop quality improvement, and has important value for increasing the content of branched chain amino acid of crops and improving dietary nutrition. The HvBCAT1 gene as well as a recombinant vector, recombinant bacteria and transgenic plants thereof have good application prospects in the fields of food and feed industry.

The invention discloses application of aloe polysaccharide in regulation and control of mitochondrial energy metabolism, and belongs to the field of new application of aloe polysaccharide. The aloe polysaccharide can regulate mitochondrial energy metabolism to be converted into anabolism, promote the content of protein and amino acid in cells and promote wound healing. The preparation method of the aloe polysaccharide comprises the following steps: removing two ends of fresh aloe barbadensis leaves, and soaking the fresh aloe barbadensis leaves with pure water for 1-2 days; washing with water and removing skin to obtain aloe gel to be ground; shearing the aloe vera gel to be ground in an automatic gel grinding device to finish gel grinding, so as to obtain aloe polysaccharide feed liquid; and putting the aloe polysaccharide feed liquid into a stirring tank, stirring at 4-15 DEG C, carrying out light treatment for at least 24 hours, and then collecting the aloe polysaccharide pervaporation membrane on the surface of the liquid in a high-temperature membrane forming tank at 75-95 DEG C, thereby obtaining the aloe polysaccharide pervaporation membrane.

The application discloses a high-sweetness-amino-acid-content Flammulina velutipes variety and a MNP molecular identification method and application thereof. The Flammulina velutipes variety has excellent properties, the cap is not easy to open, the content of sweet amino acids is high, the diversified market demand is met, the factory annual bottle cultivation is suitable, and the Flammulina velutipes variety has a good application and popularization prospect. The MNP molecular fingerprint of the Flammulina velutipes variety 'Shangyan A111' is comprehensive multiple amplification and sequencing technology, sequence analysis of all marker sites of multiple samples can be performed at one time, compared with ISSR, RAPD, SSR and other molecular markers and mushrooming test, the MNP molecular fingerprint has the advantages of high throughput, multi-target, high sensitivity and high precision. The MNP molecular fingerprint of the Flammulina velutipes variety 'Shangyan A111' has specificity and specificity in identifying the Flammulina velutipes strain 'Shangyan A111'.

The application of a soybean GmGASA1 gene, the application relates to the application of a gibberellin regulation related protein GmGASA1 coding gene of soybean.The application of the soybean GmGASA1 gene, namely the application of improving the protein content, the seed amino acid content and the grain weight of plants; the application of the soybean GmGASA1 gene in plant breeding; the application of the soybean GmGASA1 gene in cultivating transgenic plants.The experimental results show that three transgenic lines are obtained by overexpressing the GmGASA1 gene in soybean, the protein content and the grain weight of the transformed soybean seeds are significantly improved compared with the seeds of the untransformed receptor plants, which indicates that the GmGASA1 gene can regulate the protein content, the seed amino acid content and the grain weight of seeds.The GmGASA1 gene can be used for improving the quality of soybean and increasing the yield of soybean.The application of the GmGASA1 gene can effectively realize the breeding of new soybean varieties and the creation of new germplasm.

The application discloses a cultivation method for improving selenium content of Auricularia auricula, and comprises a cultivation material for improving selenium content of Auricularia auricula, wherein the cultivation material is obtained by mixing a slow-release sodium chlorate composite material and a slow-release selenium-containing mineral material with carbon nutrition, nitrogen nutrition and mineral nutrition respectively. In the application, the sodium chlorate and selenium are respectively prepared into specific slow-release systems, and the slow-release reaction in the cultivation material generates selenite which is easy to be absorbed and utilized by Auricularia auricula, so that the selenite concentration in the cultivation material is regulated, the growth of Auricularia auricula is promoted, the yield of Auricularia auricula is improved, the quality of Auricularia auricula is significantly improved, the selenium content and the amino acid content of Auricularia auricula are both significantly increased, and the cultivation material can be used for seedling cultivation of Auricularia auricula with high selenium content, so as to obtain high-quality seedlings of Auricularia auricula with high selenium content.

The invention discloses a method for fermenting vinasse feed through mixed bacteria, and belongs to the field of microbial fermentation. The invention firstly provides a microbial agent for increasing the content of crude protein in fermented vinasse. The microbial agent contains a bacillus subtilis liquid, a corynebacterium glutamicum liquid, a saccharomyces cerevisiae liquid, an enterococcus faecalis liquid and a bifidobacterium animalis liquid. The invention also provides an application of the microbial agent in preparation of a fermented vinasse feed. The contents of crude protein, crude fat and amino acid in the fermented vinasse feed are increased. Through mixed fermentation, the content of amino acid in the vinasse is increased, the content of lysine is increased by 108.33%, and the content of threonine is increased by 59.09%.

This invention discloses a method for producing amino acid-containing water-soluble fertilizer using sugarcane molasses and organic oilseed cake. The method involves first establishing an equation relating the composting time of organic oilseed cake to influencing factors, then performing material mixing, enzymatic hydrolysis, centrifugation, filtration, enzyme inactivation, preservation, and adjustment of the effective component content to obtain the amino acid-containing water-soluble fertilizer of this invention. This invention can rapidly compost organic oilseed cake within 12 hours, resulting in a stable fertilizer product with a storage period of ≥3 months. It will not undergo further enzymatic hydrolysis to produce odors or secondary metabolites, and its solubility is ≥95%, which is beneficial for the promotion of fertigation technology. The fertilizer has a high amino acid content, and the nutrient content can be adjusted as needed, significantly improving crop yield and quality after application.

A high-purity yeast protein and a preparation method therefor, wherein the method comprises the following steps: inoculating an activated yeast strain into a liquid seed culture medium, culturing same in a shaker, then inoculating same into a fermentation culture medium for culturing, and centrifuging and washing same to obtain a yeast strain; preparing the yeast strain into a suspension solution, and performing a heat treatment to collect a precipitate; preparing the precipitate into a suspension, adding 5-50 U / mL of dextranase and 5-50 U / mL of cellulase at a pH of 5.0-7.0 and 30-60°C, and performing enzymatic hydrolysis to obtain a first enzymatic hydrolysate; adding a non-ionic solubilizer to the first enzymatic hydrolysate at a certain pH, and incubating the resulting mixure at a certain temperature to obtain a second enzymatic hydrolysate; and subjecting the second enzymatic hydrolysate to solid-liquid separation, washing and drying to obtain a high-purity yeast protein. The method reduces the possibility of protein denaturation. The final yeast protein product has a low dietary fiber content, a high branched-chain amino acid content and a high protein content, and exhibits good nutritional value and processing characteristics.

The invention relates to the technical field of amino acid detection, and particularly discloses a method for detecting the content of amino acid in a sodium hyaluronate solution, which is wide in application scene, reliable in determination result and easy to operate. The detection method comprises the following steps: preparing a plurality of standard solutions with different concentrations and containing glycine, proline, alanine, leucine, valine and lysine acetate to obtain a standard series of solutions, and deriving the standard series of solutions to obtain a standard solution to be detected; pretreating and deriving a sodium hyaluronate solution to be detected to obtain a sample to be detected; carrying out liquid chromatography detection on each amino acid in the standard to-be-detected solution and the to-be-detected sample by adopting a liquid chromatography, drawing a standard curve of each amino acid, and calculating a linear regression equation of each standard curve; and calculating the content of each amino acid according to the peak area of each amino acid in the sample and the corresponding linear regression equation.

This invention discloses a coat-enhancing composition for improving the quality of cat fur and its application, belonging to the field of pet food technology. The composition uses a high proportion of fresh chicken meat and fresh bone-in chicken thigh meat as the main protein source, combined with krill oil, fresh chicken liver, various natural plant powders, and compound nutrients, formulated in a scientific ratio. Animal experiments have verified that this invention can significantly increase the amino acid content of cat fur, improve fur luster and saturation, enhance tensile strength, and optimize hair cuticle structure, without adversely affecting the cat's weight, blood routine, blood biochemistry, or other health indicators, demonstrating safety and high efficacy. This invention can be widely applied to the preparation of functional fresh meat baked food for improving the quality of cat fur.

Belonging to the technical field of tea processing, the invention discloses an improved tea processing technology, which comprises the following steps: (1) fixation pretreatment; (2) fixation; (3) spreading for cooling; (4) rolling; and (5) drying. According to the improved tea leaf processing technology, the tea leaves finally prepared through the method are remarkable in amino acid content and tea polyphenol content, high in nutrition and health care value and relatively low in caffeine and water extract content, the bitterness of the tea leaves is effectively reduced, and the quality of the tea leaves is improved to a certain degree.

The invention discloses dark baked barley rich in wheat fragrance as well as a preparation method and application of the dark baked barley, and belongs to the technical field of wheat making. Comprising the following steps: selecting barley of which the basic amino acid content is more than or equal to 30mg / L and the lignin content is less than or equal to 100mg / g as The barley baking method is characterized in that barley is baked in a sectional manner, and the barley baking method comprises the following steps: in the first stage, the barley is heated to 50-55 DEG C at a constant speed, and the water content of the barley is 12-15%; in the second stage, the temperature is rapidly increased to 90 DEG C, and the water content of the barley is reduced to 8-10%; in the third stage, the baking temperature continues to be increased to 210-220 DEG C, and baking is conducted for 20-30 min; in the fourth stage, when the crispness of the baked barley reaches 98% or above, the baking temperature is lowered to 190-210 DEG C, and then the baked barley is rapidly cooled to the room temperature. The preparation method is applied to the aspect of drinks, and solves the problems that although the color of the baked barley obtained by the existing dark-color baked barley preparation process meets the requirement, the barley fragrance cannot be highlighted due to the low pyrazine content, the smoky flavor is large due to the high guaiacol content, and the technical requirement that pure and rich barley fragrance can be obtained by extracting a small amount of the baked barley cannot be met.

The invention discloses a fish gelatin jelly gel performance evaluation method based on multi-scale correlation analysis, which comprises the following steps: extracting multi-scale characteristics of molecular characteristics, network structures and macro performance of three fish gelatin jellies, performing data correlation based on three dimensions of molecular characteristics-network structures-macro performance, and combining a stoichiometric analysis method to evaluate the gel performance of the fish gelatin jellies. And a multi-level systematic evaluation system is constructed. According to the method, data of molecular characteristics (secondary structure ratio, particle size, Zeta potential and amide A wave number), network structure (aperture size) and macroscopic performance (imino acid content and gel strength) are integrated to construct the relevance of molecular characteristics-network structure-macroscopic performance, and full-dimensional analysis of the quality of the fish gelatin jelly is realized for the first time. The method breaks through the limitation that the traditional method only depends on single indexes such as protein or collagen content and the like, and clarifies the regulation and control of molecular aggregation behavior, imino acid content and electrostatic interaction on the formation of a gel network.

The invention discloses application of a rice malic acid synthase gene in improving the nutritional quality of seeds. The invention reports that the OsIPMS1 and OsIPMS2 genes can regulate and control the nutritional quality in polished rice grains for the first time in rice, and tests show that the mutation of the OsIPMS1 and OsIPMS2 genes improves the content of protein and amino acid in the polished rice grains. It is proved that the OsIPMS1 and OsIPMS2 genes regulate and control the nutritional quality of rice seeds, and the genes are beneficial to cultivation of rice varieties with high protein and amino acid.

The present invention belongs to the technical field of microorganisms. Provided are Saccharomyces cerevisiae FNFH_Sc18 and the use thereof. In one aspect, provided is an engineered Saccharomyces cerevisiae strain FNFH_Sc18 for synthesizing immunonutrients β-carotene and astaxanthin, which strain has a deposit number of CGMCC No. 24838. In another aspect, provided is the use of the Saccharomyces cerevisiae. The strain uses soybean meal as a solid-state fermentation substrate, the resulting fermented soybean meal product exhibits significantly increased contents of crude proteins and amino acids, and substantially reduced anti-nutritional factors, such as oligosaccharides and urease. More importantly, the fermented soybean meal is rich in β-carotene and astaxanthin, which imparts new functional immunonutritional properties to a soybean meal protein, thereby improving the overall nutritional value of the soybean meal, and attaining a leading level in the field of developing functional fermented soybean meal by using Saccharomyces cerevisiae.

The application discloses a method for rapidly detecting amino acid content, which comprises the following steps: firstly, subjecting a substrate such as earthworm protein powder to microbial fermentation; and then rapidly detecting the free amino acid content in the fermentation liquor by means of an optimized indantrione color developing method (boiling water bath for 2-3 minutes, determination at 570 nm). The rapid detection technology is deeply combined with a strain screening process, and the amino acid production capacity of bacillus, lactic acid bacteria and mixed strains in a ratio of F14:MLB36:LAB2 (2:3:3) is utilized, so that high-quality strains capable of significantly improving the amino acid yield are directly screened. The application overcomes the problems of low efficiency and high cost of a traditional detection method, and provides a solution for efficient biological manufacturing and rapid detection of amino acids.

The invention discloses a bacillus subtilis strain for producing amide compounds and application of the bacillus subtilis strain. The preservation number of the bacillus subtilis DZNBS-11-032 is CGMCC (China General Microbiological Culture Collection Center) NO.29593, and the preservation number of the bacillus subtilis DZNBS-11-032 is CGMCC NO.29593. The invention provides bacillus subtilis DZNBS-11-032 capable of generating amide compounds, and the bacillus subtilis DZNBS-11-032 is added into a feed for culturing crisped grass carps, so that the content of amino acids in muscle fat and the content of highly unsaturated fatty acids can be increased, and the interest of people in the application of the bacillus subtilis in the culture of the crisped grass carps can be stimulated.

The present invention provides a culture medium for cultivating Agaricus blazei Murr. rich in selenium amino acids and β-glucan, Agaricus blazei Murr., and its preparation method and application. The raw materials for preparing the culture medium for cultivating Agaricus blazei Murr. rich in selenium amino acids and β-glucan include the following components in parts by mass: Cardamine violifolia 4 - 12 parts; β-glucan 2 - 8 parts; corncob 6 - 14 parts; corn straw 15 - 25 parts; reed 12 - 18 parts; bamboo chips 12 - 18 parts; cow dung 15 - 25 parts; sheep dung or rapeseed cake 12 - 18 parts; rice bran 2 - 10 parts; lime 0.5 - 5 parts; superphosphate 0.5 - 5 parts. The selenium amino acid content in the fruiting bodies of Agaricus blazei Murr. cultivated with this culture medium is as high as 160 - 214 mg / kg, and the organic selenium content is higher than 90%. The active ingredient complex extracted from this Agaricus blazei Murr. contains rich nutrients such as selenium amino acids, selenocysteine, selenomethionine, selenoprotein, β-glucan, various vitamins, and trace elements.

The invention belongs to the technical field of forest tree genetic engineering breeding, and particularly relates to a CbuLBD19 gene for regulating and controlling root system development and nitrogen absorption and utilization of catalpa bungei and application of the CbuLBD19 gene. The nucleotide sequence of the CbuLBD19 gene is as shown in SEQ ID No.7, and the amino acid sequence coded by the CbuLBD19 gene is as shown in SEQ ID No.8. It is found that overexpression of the CbuLBD19 gene can cause reduction of the total root length and the root biomass of catalpa bungei. Meanwhile, the net absorption rate of nitrate ions in the root system, the activity of nitrate reductase (NR) and the content of amino acid are reduced, and the comprehensive expression is that nitrogen metabolism is reduced. The negative regulation and control function of the CbuLBD19 gene on the nitrogen absorption and utilization of the catalpa bungei is defined, and a technical route for improving the nitrogen absorption and utilization of the catalpa bungei by reducing the expression of the CbuLBD19 gene is provided.

Plants, plant parts, and a population of plants or plant parts comprising reduced BIG SEEDS (BS) activity, and compositions and methods of producing such plants, plant parts, and a population of plants and plant parts are provided. The plants, plant parts, or population can have a genetic mutation or a modification of the DNA methylation pattern that reduces the BIG SEEDS activity, which can be located at least partially in at least one BS gene or homolog or in its regulatory region. The plants, plant parts, and population can have increased organ size and / or protein or amino acid content. Also provided are seed, protein, and / or oil compositions and food and beverage products produced from the plants or plant parts provided herein.

To produce tobacco which provides consumers with new flavors and sensory experiences while still maintaining commercially acceptable yields and traits.SOLUTION: A plant cell comprising (i) a polynucleotide comprising or consisting of a sequence having at least 90% sequence identity to a specified nucleic acid sequence, (ii) a polypeptide encoded by the polynucleotide described in (i), (iii) a polypeptide comprising or consisting of a sequence having at least 90% sequence identity to a specified amino acid sequence, or (iv) a construct, vector, or expression vector comprising the isolated polynucleotide described in (i). Also provided is a tobacco product comprising the plant cell.SELECTED DRAWING: None