The invention provides a novel fourth-generation infant formula. The novel fourth-generation infant formula comprises the following ingredients: lactose, desalted whey powder, 1,3-dioleoyl 2-palmitoyl triglyceride, hydrolyzed whey protein, fructo-oligosaccharide, galactooligosaccharide, mineral substance premix compound, casein hydrolysate, whey protein concentrate, soyabean lecithin, vitamin premix, lactoferrin, L-tyrosine, nucleotide, L-tryptophan, L-methionine and the like. The infant formula is added with appropriate amount of 1,3-dioleoyl 2-palmitoyl triglyceride, appropriate amount of protein hydrolysate, monomer amino acid and the like, mineral contents such as phosphorus, sodium and the like in milk powder are reduced, therefore, the fat structure, the amino acid composition, the amino acid content and the osmotic pressure in the milk powder are close to those of the breast milk, the infant formula is balanced and comprehensive in nutrition and is appropriate in composition, all substances have synergistic effect, the bioavailability of protein and the fat is effectively improved, and the infant formula can promote digestive absorption and skeletal development of babies and can reduce the visceral organ burden.