25 results about "Amino acid content" patented technology

This invention discloses a method for producing amino acid-containing water-soluble fertilizer using sugarcane molasses and organic oilseed cake. The method involves first establishing an equation relating the composting time of organic oilseed cake to influencing factors, then performing material mixing, enzymatic hydrolysis, centrifugation, filtration, enzyme inactivation, preservation, and adjustment of the effective component content to obtain the amino acid-containing water-soluble fertilizer of this invention. This invention can rapidly compost organic oilseed cake within 12 hours, resulting in a stable fertilizer product with a storage period of ≥3 months. It will not undergo further enzymatic hydrolysis to produce odors or secondary metabolites, and its solubility is ≥95%, which is beneficial for the promotion of fertigation technology. The fertilizer has a high amino acid content, and the nutrient content can be adjusted as needed, significantly improving crop yield and quality after application.

A high-purity yeast protein and a preparation method therefor, wherein the method comprises the following steps: inoculating an activated yeast strain into a liquid seed culture medium, culturing same in a shaker, then inoculating same into a fermentation culture medium for culturing, and centrifuging and washing same to obtain a yeast strain; preparing the yeast strain into a suspension solution, and performing a heat treatment to collect a precipitate; preparing the precipitate into a suspension, adding 5-50 U / mL of dextranase and 5-50 U / mL of cellulase at a pH of 5.0-7.0 and 30-60°C, and performing enzymatic hydrolysis to obtain a first enzymatic hydrolysate; adding a non-ionic solubilizer to the first enzymatic hydrolysate at a certain pH, and incubating the resulting mixure at a certain temperature to obtain a second enzymatic hydrolysate; and subjecting the second enzymatic hydrolysate to solid-liquid separation, washing and drying to obtain a high-purity yeast protein. The method reduces the possibility of protein denaturation. The final yeast protein product has a low dietary fiber content, a high branched-chain amino acid content and a high protein content, and exhibits good nutritional value and processing characteristics.

This invention discloses a coat-enhancing composition for improving the quality of cat fur and its application, belonging to the field of pet food technology. The composition uses a high proportion of fresh chicken meat and fresh bone-in chicken thigh meat as the main protein source, combined with krill oil, fresh chicken liver, various natural plant powders, and compound nutrients, formulated in a scientific ratio. Animal experiments have verified that this invention can significantly increase the amino acid content of cat fur, improve fur luster and saturation, enhance tensile strength, and optimize hair cuticle structure, without adversely affecting the cat's weight, blood routine, blood biochemistry, or other health indicators, demonstrating safety and high efficacy. This invention can be widely applied to the preparation of functional fresh meat baked food for improving the quality of cat fur.

This invention discloses a hoof protein product and its preparation method, belonging to the field of component separation and extraction; using hoof as raw material, the method includes the following steps: (1) hoof pretreatment: the hoof is washed, crushed and sieved to obtain hoof powder (2) subcritical extraction: the hoof powder is taken and pure water containing food-grade acid-base regulator is added, and a reaction solution is prepared by subcritical hydrolysis reaction (3) the reaction solution is centrifuged, separated, dialyzed and dried to obtain hoof protein; the extracted hoof protein is rich in total essential amino acids (more than 40 g / 100g), among which the content of essential amino acids such as histidine, leucine, isoleucine, threonine and cysteine ​​exceeds the content of ideal protein amino acids recommended by WHO / FAO, and has high nutritional value. The essential amino acid index (EAAI), biological value (BV) and nutritional index (NI) are close to the gold standard of egg protein, and the digestibility is high. It has good functional characteristics and has the potential to be a protein supplement component; the preparation process of this invention is scientific and reasonable, easy to operate, low in cost, can realize continuous production, and has no pollution to the environment.

The invention belongs to the field of detection and analysis, and relates to a method for determining amino acid, protein and lignin in a sample, which comprises the following steps: extracting the sample by using an acetic acid solution containing ethyl acetate, and carrying out solid-liquid separation to obtain a first liquid phase and a first solid phase; measuring a first liquid phase by liquid chromatography-tandem mass spectrometry, and calculating the content of amino acid; extracting the first solid phase by using an ammonia-containing dimethyl sulfoxide solution to obtain extract liquor and residues; measuring the content of protein in the extract liquor by a coomassie brilliant blue method; carrying out acidolysis on the residues by using a trifluoroacetic acid solution to enable the concentration of trifluoroacetic acid in an acidolysis product to reach 8-12% by volume, carrying out acidolysis at the microwave power of 250-350W and the temperature of 115-130 DEG C, and carrying out solid-liquid separation to obtain a second liquid phase and a second solid phase; measuring the absorbance of the second liquid phase by using an ultraviolet and visible spectrophotometer to obtain the content of the acid-soluble lignin; performing second solid-phase roasting, and obtaining the content of the acid-insoluble lignin according to the mass change before and after roasting. The method disclosed by the invention can be used for rapidly and accurately determining the contents of amino acid, protein and lignin.

This invention relates to the field of marker-assisted breeding technology, and in particular to a molecular marker related to the muscle quality traits, amino acid content, and fatty acid content of beef cattle, and its application. Through sequencing and polymorphism analysis of 106 Yanhuang cattle, this invention identified an SNP site located in exon 2 of the CRYAB gene. Based on this, a molecular marker was developed. This marker not only enables rapid and accurate typing of the CRYAB gene in beef cattle (such as Yanhuang cattle), but also shows a significant correlation with individual meat quality traits, amino acid content, and fatty acid content. Based on the genotype of the molecular marker, individuals with higher overall meat quality traits can be screened at an early stage, thereby improving the speed of breeding superior beef cattle.

The invention discloses a method for increasing protein content and amino acid content of phaeodactylum tricornutum and application. According to the method, a DPH gene in wild type phaeodactylum tricornutum is knocked out through a gene knockout technology, so that the total protein content and the amino acid content of the phaeodactylum tricornutum are increased, a method for knocking out the DPH gene in the wild type phaeodactylum tricornutum comprises the steps that the DPH gene is knocked out in a targeted mode through a CRISPR / Cas9 editing carrier, the nucleotide sequence of gRNA of the DPH gene is knocked out in a targeted mode through the CRISPR / Cas9 editing carrier is shown as SEQ ID No.3, and the content of the total protein in the wild type phaeodactylum tricornutum is increased. The total protein content and the essential amino acid content of the DPH gene knockout phaeodactylum tricornutum strain are remarkably improved compared with those of a wild type strain. The transformed algal strain can be used as a high-quality protein source to be applied to the field of aquaculture, the nutritional value and the transformation efficiency of feed are effectively improved, and the growth performance and the health condition of bred animals are improved. Wide market prospects and important economic values are realized.

The group of inventions relates to the field of processing oily raw material, and more particularly to a novel product in the form of a protein-sugar hydrolysate (PSH) obtained from sunflower meal, a method for producing same by enzymatic hydrolysis, and PSH-based food and feed products. The PSH contains 40-70% protein, 20-40% sugar, and at least 0.02% caffeic acid in free form or in bound form as part of chlorogenic acid isomer molecules. In addition, the PSH has a total branched-chain amino acid content of at least 30 g / kg of the final product (PSH), and an arginine content of at least 20 g / kg PSH. The resulting PSH does not contain antinutrients and serves as an excellent substitute for animal protein in food products and in diets for aquatic organisms, livestock and domestic pets. The claimed novel method is safe, environmentally-friendly, waste-free and allows flexibility in terms of the operations and ingredients used in the production process.

A selenium-enriched biotin scalp care essence spray and its preparation method belong to the field of scalp care technology. The spray components are: selenium yeast extract, pearl active peptides, hair follicle repair core enzyme complex, biotin, plant extract micropowder, and the remainder being excipients and deionized water, with a pH of 5.0-5.5. The selenium yeast extract contains ≥60% selenocysteine; the pearl active peptides have a molecular weight <1kDa and an amino acid content ≥90mg / mL; the hair follicle repair core enzyme complex contains ≥2000U / mg superoxide dismutase and ≥50U / mg transglutaminase; and the plant extract micropowder is extracted using supercritical CO2. The use of ultrasonic high-pressure homogenization, iontophoresis encapsulation, and nitrogen-filled aluminum bottle packaging technology solves the problems of low transdermal penetration rate of active ingredients (traditional formulations <8%, this invention increases it to 28%) and poor stability.

A general-purpose selenium-enriched pearl-based basic skincare mother liquor, by weight, is composed of pearl active peptides, selenium yeast extract, and an enzyme complex in a weight ratio of 1:(0.00625~0.1):(0.0375~0.25). The pearl active peptides have a molecular weight <1kDa, an amino acid content ≥90mg / mL, and are colorless and transparent. The selenium yeast extract has a particle size <80nm, selenocysteine ​​≥60%, and a Zeta potential of +18 to +23mV, and is a colorless and transparent dispersion. The enzyme complex contains SOD ≥2000U / mg and CAT ≥1500U / mg, and is encapsulated in a chitosan-sodium alginate bilayer, making it colorless and semi-transparent. This basic skincare mother liquor addresses the problems of low dissolution and delivery rates of pearl's effective components, poor stability of selenium components, and lack of multi-effect synergistic mechanisms. It can be used to rapidly prepare various types of cosmetics, such as serums (for external or oral use) and masks.

The invention discloses kudzu root ferment and the preparation method thereof. The preparation method comprises the following steps: preparing kudzu root into kudzu root juice; adjusting the pH of the kudzu root juice; inoculating with Lactobacillus plantarum Z38 and yellow rice wine yeast; performing ultrasonication and conducting the first fermentation to obtain the primary fermentation broth; adding a carbon source to the primary fermentation broth and adjusting the pH; inoculating again with Lactobacillus plantarum Z38 and yellow rice wine yeast followed by a second fermentation to obtain the secondary fermentation broth; and adding a stabilizer and a flavoring agent to the secondary fermentation broth and adjusting the pH. The method of the present invention significantly enhancing the acetaldehyde dehydrogenase activity, pueraria flavonoids content, physicochemical components, and amino acid levels in the kudzu root ferment. This process improves the nutritional value, flavor profile, and alcohol-sobriety capacity of the kudzu root ferment.

This invention discloses an amino acid assay kit based on the o-phthalaldehyde (OPA) method, its preparation method, and its application. Belonging to the field of amino acid detection and analysis technology, it is used for rapid and accurate quantitative detection of amino acids in samples. The amino acid assay kit can not only measure the total amino acid content but also analyze specific amino acids and dipeptides such as alanine glutamine, meeting diverse analytical needs. This amino acid assay kit is suitable for batch testing and supports high-throughput laboratory applications. By optimizing the reagent formulation and operating procedures, this invention effectively simplifies the detection steps, improves detection sensitivity and specificity, and fills the gap in the market for OPA-based assay kits. This invention can be widely applied in nutrition, medicine, clinical diagnostics, and drug development, providing a convenient and efficient amino acid analysis tool for life science and medical research.

The application belongs to the technical field of microorganisms and particularly relates to a Pichia kudriavzevii and application thereof. Pichia kudriavzevii The Pichia kudriavzevii is Pichia kudriavzevii AMCC 31367 (Pichia kudriavzevii CCTCC NO: M 20251680). The Pichia kudriavzevii has the characteristics of multi-environment tolerance, broad-spectrum substrate applicability and high protein and amino acid content, and can tolerate high-concentration lactic acid, and the yeast can still grow under the lactic acid concentration of 170 g / L, thereby solving the problems of limited raw material selection, low production efficiency and high cost in the development of microbial cell protein, and the strain has application potential in the fields of biological materials, microbial engineering, environmental governance, feed and food processing.

This invention discloses a method and apparatus for evaluating the digestibility of compressed dry food. The method includes: using an in vitro dynamic human gastrointestinal digestive system to simulate the digestion of compressed dry food and standard food, respectively, to obtain corresponding digestive juices; measuring the protein content sequence, amino acid content sequence, apparent viscosity value sequence, and protein digestibility sequence of the digestive juices corresponding to the compressed dry food and the standard food, respectively; and performing quantitative evaluation of the digestibility of the digestive juices corresponding to the compressed dry food and the standard food to obtain the digestibility evaluation value of the compressed dry food.

The application discloses a kind of compressed dry food energy supply stability evaluation method and device, the method comprises: using in vitro dynamic human gastrointestinal tract digestive system, compressed dry food and standard food are respectively simulated digestion processing, respectively corresponding digestive juice is obtained;The corresponding digestive juice of the compressed dry food and the corresponding digestive juice of standard food are measured to obtain corresponding protein content sequence, amino acid content sequence, carbohydrate content sequence, fat content sequence respectively;The protein content sequence, amino acid content sequence, carbohydrate content sequence, fat content sequence are carried out energy supply stability evaluation processing, and the energy supply stability evaluation value of compressed dry food is obtained.

A strain of Bacillus licheniformis, its screening method and application, in the field of microbial fermentation technology, wherein the Bacillus licheniformis is classified as Bacillus licheniformis ( Bacillus licheniformis BL-2501, with accession number CCTCC NO: M20251206, accession date May 28, 2025. In this invention, BL-2501, using corn stalks as a carbon source for fermentation, achieved degradation rates of 38.52% for NDF and 31.61% for ADF. During the fermentation of corn stalks to prepare feed, it effectively increased the crude protein content and reducing sugar content of the feed, and significantly improved the sulfur-containing amino acid content, with methionine and cysteine ​​reaching 5.88 mg / g and 0.73 mg / g, respectively, thus significantly improving the nutritional value of the feed.

The application discloses a preparation method of bovine placenta peptide and application thereof, and belongs to the technical field of biological products. The bovine placenta peptide is prepared from the placenta of a Galla Sahiwal cow, and the specific preparation steps comprise raw material pretreatment, complex enzymolysis, enzyme inactivation and separation, desalting and decoloration, concentration and drying. The complex enzymolysis adopts trypsin and neutral protease, and the adding ratio of the trypsin to the neutral protease is 3:2. More than 90% of the peptide segments in the obtained bovine placenta peptide have molecular weights distributed in the range of 500-1500 Da, the protein content is greater than 90%, and the free amino acid content is less than 3%. The bovine placenta peptide has a narrow peptide molecular weight distribution range, strong antioxidant and immunoregulatory activities, and health care functions of preventing and assisting in treating lung nodules and premature ovarian failure.

The present application belongs to the technical field of molecular markers and animal breeding, and particularly relates to a molecular marker related to the content of glycine, alanine and histidine in beef cattle muscle and application thereof. The present application provides a molecular marker located at the 18184950th base of chromosome 17 of a beef cattle reference genome GCF_002263795.3 version, having C / T polymorphism, which can not only realize rapid and accurate genotyping of beef cattle population SETD7 gene, but also based on the significant correlation between the site and the amino acid content of individuals, the beef cattle individuals with high amino acid content can be screened early, so as to improve the selection speed of superior beef cattle. Compared with the traditional single genotype selection, the present application has higher accuracy and significant advantages for screening beef cattle individuals with high amino acid content, saving feeding cost and improving the selection of superior beef cattle. The amino acid in the present application is one or more of glycine, alanine and histidine.

This invention discloses a strain of Auricularia auricula-judae HN-7 ( Auricularia This strain (species name) and its applications belong to the field of edible fungi technology. It was deposited at the China Center for Type Culture Collection (CGMCC) on November 14, 2025, with accession number CGMCC No. 42368. The HN-7 strain exhibits rapid mycelial growth, strong resistance to mold, small auricle bases, large leaves, numerous folds on the ventral side, and a grayish-yellow dorsal surface with prominent villi. The polysaccharide and amino acid contents in its fruiting bodies are 28.9% and 39.5% higher than the control strain 781, respectively, indicating higher nutritional value and yield. This strain has significant application value in the cultivation of Auricularia auricula-judae. It enriches the germplasm resources of Auricularia auricula-judae and helps improve the economic benefits of the Auricularia auricula-judae industry.

The application relates to the technical field of genetic engineering, in particular to a soybean GmPEAMT1 and GmPLMT gene and an application of the coded protein in regulating soybean seed quality. GmPEAMT1 and GmPLMT The nucleotide sequence of the gene is shown in the sequence table SEQ ID NO. 1-2, GmPEAMT1 and GmPLMT The amino acid sequence of the protein coded by the gene is shown in the sequence table SEQ ID NO. 3-4. Regulating soybean seed quality includes reducing the water content of soybean seeds, and increasing the total protein content and amino acid content of soybean seeds. Advantages are that, by using the CRISPR-Cas9 technology to knockout the GmPEAMT1 and GmPLMT gene at the same time, a GmPEAMT1 and GmPLMT double-gene-edited soybean is constructed, materials are provided for soybean quality improvement, and the situation that the oil content and the protein and amino acid content in soybean seeds are negatively correlated in traditional molecular breeding is broken.

The application discloses a kind of compressed dry food digestion performance prediction method and device, the method comprises: the component information set of compressed dry food is obtained by acquisition;The component information set includes protein content information, mineral content information, starch content information and fat content information;The preset digestion performance prediction model is trained and handled, and the digestion performance prediction model that training is completed is obtained;Using the digestion performance prediction model that training is completed, the component information set of compressed dry food obtained by acquisition is fused and predicted and handled, and the digestion performance prediction information set is obtained;The digestion performance prediction information set includes protein content prediction value, amino acid content prediction value, apparent viscosity prediction value, protein digestion rate prediction value.

The present application relates to the technical field of nondestructive testing of agricultural products, and particularly relates to a nondestructive testing model for amino acid content of shelled walnut and an amino acid content detection method thereof. The construction of the detection model first screens an optimal pretreatment method through spectral data of walnut kernels, then establishes a mapping relationship between the spectrum of shelled walnut and the spectrum of walnut kernels to correct the interference of the shell, and then processes the corrected near-infrared spectrum using the optimal pretreatment method. Furthermore, the characteristic wavelength screening is used to extract characteristic wavelength variables highly correlated with the amino acid content from the spectrum after pretreatment. Finally, based on the preferred characteristic wavelength subset, the amino acid content detection model suitable for shelled walnut is constructed by using the amino acid content basic detection model. The detection model does not need to damage the walnut shell or use chemical reagents, and realizes true nondestructive testing, which meets the actual needs of modern walnut industry for online, rapid and nondestructive testing of a large number of samples.

The application provides application of a CsPPC3 gene in regulation of carbon and nitrogen metabolism of cucumber fruits, and takes a main-effect gene CsPPC3 coding phosphoenolpyruvate carboxylase (PEPC) in carbon fixation of cucumber fruits as a research target, the function of the CsPPC3 gene in carbon fixation of fruit photosynthesis and carbon and nitrogen metabolism is researched by constructing overexpression and gene knockout transgenic plants, it is found that the transgenic plant of the overexpression of the CsPPC3 gene has the following characteristics: the amount of CO2 released by respiration of the fruit is reduced, the CO2 refixing capacity is enhanced, the single fruit weight is increased, the content of malic acid, soluble sugar and starch is increased, and the content of amino acid is reduced; the transgenic plant of the knockout of the CsPPC3 gene has the following characteristics: the amount of CO2 released by respiration of the fruit is increased, the single fruit weight is reduced, the content of malic acid, soluble sugar and amino acid is reduced, the content of starch is increased, and the CO2 refixing capacity is reduced. The application has practical guiding significance for cultivating transgenic plants with high CO2 fixation efficiency.

This invention provides a branched-chain amino acid transaminase gene and its applications, belonging to the field of genetic engineering. This invention is the first to discover the branched-chain amino acid transaminase gene HvBCAT3, which shows a significant positive correlation with leucine (Leu) accumulation in crops. This invention provides an important target gene, HvBCAT3, for crop quality improvement, and is of great value in increasing the content of branched-chain amino acids in crops and improving dietary nutrition. The HvBCAT3 gene, its recombinant vectors, recombinant bacteria, and transgenic plants all have promising applications in the food and feed industries.