329 results about "Amino acid content" patented technology

A non-alcoholic beer-flavored beverage is disclosed that has an aromatic amino acid content of 50 ppm or more and has an enhanced drinking experience.

The invention relates to the field of microorganisms, and discloses an Aspergillus oryzae strain and application thereof.The Aspergillus oryzae can produce protease, aminopeptidase, glutaminase, cellulase, amylase and pectinase at high yield, and when the Aspergillus oryzae is applied to soy sauce brewing, the utilization rate of raw materials can be increased, the content of amino acid in soy sauce can be increased, and the flavor of the soy sauce can be increased.

The invention relates to the field of microorganisms, and discloses an Aspergillus tubingensis strain and application thereof in preparation of meal seasonings, the Aspergillus tubingensis strain is not subjected to mutagenesis and acclimatization and has the capacity of high yield of glutaminase, feruloyl esterase, glucosidase, pectinase and protease, and when the Aspergillus tubingensis strain is applied to soy sauce brewing, the yield of soy sauce is increased. The high-value utilization of the meal raw materials can be realized, the amino acid content in the soy sauce is increased, and the flavor and nutritional value of the soy sauce are enhanced.

The invention relates to a preparation method of a cultivation material for cultivating selenium-rich black fungus, which comprises the following steps: respectively preparing a slow-release sodium chlorate composite material and a slow-release selenium-containing mineral material, and then mixing with carbon nutrition, nitrogen nutrition and mineral nutrition to obtain the cultivation material. The slow-release selenium mineral material is a polymer-coated slow-release selenium mineral material prepared by the following steps: preparing a selenium mineral core material from selenium powder, gypsum, calcium superphosphate, monopotassium phosphate, calcium sulfate, diatomite, nano silicon dioxide and a surfactant OP-10, preparing a coating material, adding the selenium mineral core material, and soaking with a Bis-Tris buffer solution. Sodium chlorate and selenium are respectively prepared into specific slow release systems, and are slowly released in the cultivation material to react to generate a seleninic acid component which can be easily absorbed and utilized by the black fungus, so that the concentration of seleninic acid in the cultivation material is regulated, the growth of the black fungus is promoted, the yield of the black fungus is increased, the quality of the black fungus is remarkably improved, and the cultivation cost is reduced. The selenium content and the amino acid content in the black fungus are obviously increased.

The invention discloses saccharomyces cerevisiae FNFHSc18 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain FNFHSc18 for synthesizing immune nutrients-beta-carotene and astaxanthin, and the preservation number of the saccharomyces cerevisiae engineering strain is CGMCC (China General Microbiological Culture Collection Center) No.24838; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid state fermentation substrate, the content of crude protein and amino acid of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, more importantly, the fermented soybean meal is rich in beta-carotene and astaxanthin, and the content of the beta-carotene and the astaxanthin is greatly reduced. The soybean meal protein obtains new functional immune nutrition, so that the overall nutritional value of the soybean meal is improved, and the soybean meal reaches a leading level in the field of developing functional fermented soybean meal by utilizing saccharomyces cerevisiae.

The invention discloses a preparation process of high-efficiency absorption fish protein peptide and application of the high-efficiency absorption fish protein peptide in fertilizer, and belongs to the technical field of marine organisms. The aquatic product protein is directly used as a fermentation raw material, and the content of amino acid in the fish protein biological peptide is remarkably increased by utilizing the synergistic fermentation effect of microorganisms and assisted by biological enzymolysis, so that the absorptivity of the fish protein peptide is increased; the prepared fish protein peptide is used in the fertilizer to prepare the fish protein peptide functional fertilizer, the nutritional value and the utilization rate of the fertilizer are remarkably improved, and the fish protein peptide functional fertilizer has the remarkable effects of growth promotion, disease resistance, stress resistance and the like. According to the method, a conventional fish protein peptide preparation method of firstly preparing fermentation enzyme liquid and then performing biological enzymolysis is abandoned, the efficient absorption fish protein peptide is prepared by adopting a process of directly fermenting aquatic protein, a fermentation process and an enzymolysis process are integrated, and compared with a conventional process, the method has the advantages of simple production process, low production cost and the like; the method has significant effects and meanings for promoting green preparation of the fish protein peptide and efficient application of the fish protein peptide in fertilizers.

The invention provides a branched chain amino acid transaminase gene and application thereof, and belongs to the field of gene engineering. According to the invention, the branched chain amino acid transaminase gene HvBCAT5 is found for the first time, and the gene has obvious positive correlation with the accumulation of valine (Val), isoleucine (Ile) and leucine (Leu) of crops. The invention provides an important target gene HvBCAT5 for crop quality improvement, and has important value for increasing the content of branched chain amino acid of crops and improving dietary nutrition. The HvBCAT5 gene as well as a recombinant vector, a recombinant bacterium and a transgenic plant thereof have good application prospects in the fields of food and feed industry.

A preparation method of a black fungus cultivation material containing pleurotus eryngii mushroom dregs comprises the following steps: adding the pleurotus eryngii mushroom dregs into dry chicken manure to form a fermentation base material, then spraying a tannic acid solution for pretreatment, then inoculating microorganisms for fermentation treatment to obtain pleurotus eryngii fermentation mushroom dregs, and then adding the fermentation base material into the fermentation base material for fermentation treatment to obtain the black fungus cultivation material containing the pleurotus eryngii mushroom dregs. Mixing the pleurotus eryngii fermentation mushroom dregs, wood chips, bran, soybean cake powder, gypsum powder and calcium superphosphate to form the cultivation material. Pleurotus eryngii fermentation mushroom residues are pretreated with a tannic acid solution with a specific concentration, then specific fermentation treatment is conducted, the obtained fermentation mushroom residues replace part of wood chips to prepare the cultivation material, the growth rate and growth vigor of black fungus hyphae are effectively increased, the full-bag period of the hyphae is shortened, and the yield of the black fungus hyphae is increased. Meanwhile, the growth of the black fungus sporocarp is promoted, the yield and quality of the black fungus are improved, the yield of the black fungus is improved by 11.29% compared with that of a cultivation material without pleurotus eryngii mushroom dregs, the content of auricularia auricula polysaccharide and amino acid in the black fungus is remarkably increased, and the breeding quality of the high-quality black fungus is effectively promoted.

The invention belongs to the technical field of microbial fermentation, and discloses torulopsis variabilis and application thereof. Wild torulopsis variabilis is separated from soy sauce mash, a strain of torulopsis variabilis with high yield of aspartic acid, threonine, glutamic acid, valine, methionine, isoleucine, leucine and lysine is obtained through ARTP mutagenesis, the collection number of the torulopsis variabilis ZB444 is GDMCC No: 65240, the classification name of the Latin name of the torulopsis variabilis is Wickhamiella versitis, the collection number of the torulopsis variabilis ZB444 is CGMCC No: 65240, and the collection number of the torulopsis variabilis ZB444 is CGMCC No: 65240. The strain is preserved in Guangdong Microbial Culture Collection Center on October 12, 2024. The strain is applied to soy sauce fermentation, the content of various amino acids and the content of total amino acids in soy sauce can be increased, the nutritional value of the soy sauce can be increased, and meanwhile the flavor of the soy sauce is enhanced.

The invention relates to a method for detecting the content of amino acid in a pesticide through pre-column derivatization liquid chromatography, and relates to the technical field of chemical analys.The method comprises the steps that a pesticide sample to be detected is extracted, and a solution containing amino acid is obtained; then, under the optimized alkaline pH and heating conditions, 4-fluoro-7-nitro-2, 1, 3-benzoxadiazole (NBD-F) is adopted as a derivatization reagent to be subjected to a pre-column derivatization reaction with amino acid, and a stable high-fluorescence NBD-F derivative is formed; injecting the derivative liquid into a high performance liquid chromatography system provided with a phenyl bonded phase chromatographic column, and efficiently separating the NBD-F derivative by utilizing pi-pi interaction and hydrophobic interaction of the phenyl column; and finally, detecting and quantifying through a fluorescence detector. Through the synergistic effect of NBD-F derivation and the phenyl column, the separation selectivity and the detection sensitivity are remarkably improved, and the reaction efficiency and the derivative stability are guaranteed through optimized derivation conditions.

The invention belongs to the field of fertilizers, and particularly relates to a foliar fertilizer with high amino acid synthesis efficiency and a preparation method thereof.The preparation method comprises the steps that spermidine is taken to be subjected to formic acid esterification modification, and formic acid esterification spermidine is obtained; the preparation method comprises the following steps: hydrolyzing 2, 4-epibrassinolide to obtain carboxylated brassinolide; under the action of Tween, carboxylated brassinolide and formic acid esterified spermidine are combined to form a fertilizer base full of emulsified liquid drops; and adding other nutrients to obtain the foliar fertilizer. The leaf fertilizer for increasing the amino acid content of rice can increase the content of amino acids, especially lysine, in grains on the premise of increasing the yield of rice, and nitrogen absorption and utilization of the rice grains are improved.

The invention discloses an ecological planting method for remarkably improving the amino acid content of citrus, which comprises the following steps: (1) mixing and fermenting pruned citrus branches, weed straws, livestock and poultry manure and indigenous probiotic ferment powder to prepare a basic microbial fertilizer, and adding a calcium magnesium phosphate fertilizer and monopotassium phosphate to prepare a special microbial fertilizer for citrus, applying as a base fertilizer in winter; (2) after citrus pruning, in key phenological periods such as a flower bud period, a young fruit period and a fruit swelling period, an indigenous probiotic enzyme diluent with a specific dilution ratio is used for systematic foliage spraying on a tree body. The indigenous probiotic enzyme contains bacillus amyloliquefaciens, saccharomycetes, active enzymes and a plurality of nutrient elements. The method can significantly increase the total amino acid content of citrus fruits, greatly increase the yield, improve the taste of the fruits, enhance the tree body resistance, reduce the use of chemical pesticides, improve soil, and realize quality improvement, yield increase and green sustainable development of citrus planting.

The invention belongs to the technical field of detection methods, and provides a method for determining the content of amino acid in chyme in digestive tracts of livestock and poultry, which comprises the following steps: mixing a sample and a peroxyformic acid solution, and reacting to obtain an oxidation system; mixing the oxidation system, a hydrochloric acid solution and a chelating agent solution, and hydrolyzing to obtain a hydrolysis system; mixing the hydrolysis system and alkali liquor, then adjusting the pH value, and then mixing with acid liquor to obtain liquid to be detected; centrifuging the to-be-measured liquid, filtering, injecting a filtering system and the mixed amino acid standard liquid into an automatic amino acid analyzer, and measuring. According to the method, a certain amount of disodium ethylene diamine tetraacetate is added to chelate heavy metals in chyme in digestive tracts of livestock and poultry, and meanwhile, the heavy metals and redundant impurities are removed by adopting the steps of centrifuging, filtering and the like, so that the accuracy of amino acid detection is ensured, and the addicability of digestible amino acid content in daily ration is better. The hydrolysate is treated by adopting simple steps such as pH regulation and centrifugation, so that the operation process is simplified, and the operation error is reduced.

The invention discloses a processing method of fermented cottonseed meal for replacing soybean meal. The method comprises the following steps: uniformly mixing cottonseed meal, feather meal, corn starch and water to obtain a fermentation substrate, adding a first-stage fermentation inoculant into the fermentation substrate for aerobic fermentation, adding a second-stage fermentation inoculant for anaerobic fermentation after fermentation is finished, and performing low-temperature drying and crushing on a fermentation product after fermentation is finished to obtain the cottonseed meal fermented feed. The first-stage fermentation inoculant comprises candida utilis, aspergillus oryzae, papain and cellulase; and the second-stage fermentation inoculant comprises lactobacillus reuteri and lactobacillus acidophilus. According to the method, a bacterium-enzyme synergistic two-step fermentation mode is adopted, bacterium-enzyme synergistic aerobic fermentation is carried out firstly, then anaerobic fermentation is carried out with compound probiotics, and the obtained cottonseed meal fermented feed is high in crude protein and amino acid content and good in palatability, can completely replace soybean meal, can effectively regulate fat metabolism and can improve the lean meat percentage of fattening pigs.

The invention belongs to the technical field of nano material application and chemical detection, and particularly discloses a method and a kit for detecting the content of D-amino acid based on a Pt / Ti3C2Tx nano material and application of the method and the kit. The method comprises the following steps: incubating a sample to be detected and D-amino acid oxidase together; after the incubation is completed, adding a Pt / Ti < 3 > C < 2 > T < x > nano material and a chromogenic substrate TMB (Tetramethylbenzidine), and reacting under laser irradiation; and after the reaction is finished, determining the content of D-amino acid by a colorimetric detection method. The kit comprises D-amino acid oxidase, a Pt / Ti < 3 > C < 2 > T < x > nanometer material and TMB (Tetramethylbenzidine). The Pt / Ti < 3 > C < 2 > T < x > nanometer material has local surface plasma resonance effect and peroxidase-like catalytic activity, a colorimetric detection platform constructed based on the Pt / Ti < 3 > C < 2 > T < x > nanometer material is applied to clinical saliva sample detection, has the characteristics of high sensitivity, high selectivity and the like, and can provide a rapid, simple and painless new path for early screening of gastric cancer.

The invention discloses an amino acid water-soluble fertilizer and a production process thereof, and relates to the technical field of water-soluble fertilizers. Free amino acid is used as an organic nitrogen source, and the medium element calcium is combined, so that the crop quality is improved. The formula is low in cost, high in absorptivity, suitable for crop growth, simple and convenient to use and high in cost performance, and promotes the synergistic effect of nutrient elements. The method takes amino acid and calcium as raw materials, and has the characteristics of wide applicability, high output-input ratio and easiness in market promotion. The product is completely water-soluble, environment-friendly and safe, can regulate the physiological function of crops, increase the yield and amino acid content and improve the quality, and has remarkable economic benefits. In addition, activated ricinoleic acid carboxyl is combined with fucoidan enzymatic hydrolysis oligosaccharide to construct the microcapsule, so that efficient utilization of resources is realized.

The invention discloses rhodotorula mucilaginosa PL10 and application thereof in improving the quality of sweet rice wine, and belongs to the technical field of microorganisms, and the preservation number of the rhodotorula mucilaginosa PL10 is CCTCC NO: M20241214. The invention finds that the sweet rice wine cooperatively fermented by the rhodotorula mucilaginosa PL10 and the rhizopus oryzae has the highest sensory evaluation score, the content of delicate flavor and sweet amino acids in each group is the highest, the proportion of the delicate flavor and sweet amino acids in the total amino acid content is higher than 75%, the bitter amino acids are less, the types of ester compounds and aldehyde ketone compounds in each group are the highest, and the content of the sweet rice wine is the highest. Therefore, when the sweet rice wine is fermented by the rhodotorula mucilaginosa PL10, the content of bitter amino acid can be obviously reduced, the content of sweet amino acid and the content of delicious amino acid can be obviously increased, the variety of ester compounds and aldehyde ketone compounds of the sweet rice wine can be obviously increased, and the problems that the commercial sweet rice wine is boring in wine taste and fragrance and insufficient in flavor can be effectively solved. The research result provides reference and theoretical basis for preparation of the Yunnan sweet rice wine.

The invention discloses a method for preparing fish protein peptide through a biological method and application of the fish protein peptide in fertilizer, and belongs to the technical field of biology. The content of amino acid in the fish protein biological peptide is remarkably increased by utilizing the synergistic fermentation effect of microorganisms and assisting with biological enzymolysis, and the fish protein biological peptide organic fertilizer is further developed, so that the nutrition of the biological organic fertilizer is improved, the disease resistance and stress resistance of plants are improved, and the growth of the plants is promoted; therefore, an efficient and novel animal protein fertilizer is provided, and iterative updating of the fish protein biological peptide organic fertilizer is realized. The fish protein peptide prepared by the invention has the advantages of high amino acid content, degradability, no toxicity, high biocompatibility, high safety and the like, and provides a high-quality raw material for developing a fish protein peptide fertilizer capable of remarkably improving disease resistance and stress resistance of plants and promoting growth. At present, the fish protein peptide prepared by the method is applied to actual production, and remarkable economic benefits are obtained.

The invention provides a branched chain amino acid transaminase gene and application thereof, and belongs to the field of gene engineering. According to the invention, the branched chain amino acid transaminase gene HvBCAT3 is found for the first time, and the gene is obviously positively correlated with the accumulation of leucine (Leu) of crops. The invention provides an important target gene HvBCAT3 for crop quality improvement, and has important value for increasing the content of branched chain amino acids of crops and improving dietary nutrition. The HvBCAT3 gene as well as a recombinant vector, recombinant bacteria and transgenic plants thereof have good application prospects in the fields of food and feed industry.

The invention discloses a method for establishing rapid prediction of important quality indexes of crops based on near infrared spectrum and deep learning. The method comprises the following steps: preparing a green soy bean sample; determining the content of amino acid and starch in the green soy bean sample by using a specific instrument; acquiring near infrared spectrum data and preprocessing the spectrum data; constructing a deep neural network model; feature band selection is carried out on the preprocessed near infrared spectrum data, and then a test set and a training set are divided according to the proportion of 7: 3; training a deep neural network model; and inputting near infrared spectrum data on the basis of the deep neural network model trained in the step S6 so as to obtain the amino acid content and the starch content of the corresponding green soy beans. The method can be used for monitoring the content of important quality indexes in the green soy bean production process in real time, the production efficiency and the quality control are improved, a novel and efficient important quality index detection method is provided for the field of agricultural product detection, and the method has a wide application prospect.

The invention provides a branched chain amino acid transaminase gene and application thereof, and belongs to the field of gene engineering. According to the invention, the branched chain amino acid transaminase gene HvBCAT1 is found for the first time, and the gene has obvious positive correlation with valine (Val) and isoleucine (Ile) accumulation of crops. The invention provides an important target gene HvBCAT1 for crop quality improvement, and has important value for increasing the content of branched chain amino acid of crops and improving dietary nutrition. The HvBCAT1 gene as well as a recombinant vector, recombinant bacteria and transgenic plants thereof have good application prospects in the fields of food and feed industry.

The invention discloses application of aloe polysaccharide in regulation and control of mitochondrial energy metabolism, and belongs to the field of new application of aloe polysaccharide. The aloe polysaccharide can regulate mitochondrial energy metabolism to be converted into anabolism, promote the content of protein and amino acid in cells and promote wound healing. The preparation method of the aloe polysaccharide comprises the following steps: removing two ends of fresh aloe barbadensis leaves, and soaking the fresh aloe barbadensis leaves with pure water for 1-2 days; washing with water and removing skin to obtain aloe gel to be ground; shearing the aloe vera gel to be ground in an automatic gel grinding device to finish gel grinding, so as to obtain aloe polysaccharide feed liquid; and putting the aloe polysaccharide feed liquid into a stirring tank, stirring at 4-15 DEG C, carrying out light treatment for at least 24 hours, and then collecting the aloe polysaccharide pervaporation membrane on the surface of the liquid in a high-temperature membrane forming tank at 75-95 DEG C, thereby obtaining the aloe polysaccharide pervaporation membrane.

The invention belongs to the field of tea making methods, and discloses a novel fermented sour tea and a preparation method thereof. The invention uses Aspergillus niger RAF106 strain to make lightly fermented sour tea, and the method is short in time and simple in method. The free amino acid content of tea leaves obtained by fermentation by the method is significantly increased, the gallic acid content is increased and the tea leaves are rich in non-ester catechins, and the tea soup is weakly acidic and has obvious sweet aftertaste.

The preparation method comprises the following steps: (1) fresh tea leaves, distilled water, glucose and protein are mixed, the mass content of the glucose is 10%-15%, the content of the protein is 0.5-1.0 g / L, and the pH value of the fermentation liquor is adjusted to 3.5-7.0; (2) after sterilization, inoculating 3%-5% of lactobacillus and 5%-15% of saccharomycetes to obtain a mixed fermentation suspension, and carrying out primary fermentation under a closed condition at the fermentation temperature of 25-30 DEG C; (3) secondary fermentation is carried out, the fermentation temperature is 23-27 DEG C, the secondary fermentation time is 5-10 h, and the oxygen introduction amount is 0.10-0.30 vvm; and (4) carrying out solid-liquid separation to remove insoluble fermentation products and plant residues so as to obtain the black tea fermentation liquor. The fermentation liquor prepared by the preparation method of the black tea fermentation liquor is obviously improved in the aspects of polysaccharide content, tea polyphenol content, amino acid content and the like, and the small molecule polysaccharide content is improved.

The invention provides a breeding method of fast-growing high-quality Dongzhihuo. Comprising the following steps: identifying the genotype of an SNP (Single Nucleotide Polymorphism) locus which is located at the 79th site of a gene sequence as shown in SEQ ID NO.1, and if the genotype of the locus is TT type, determining that the locus is a candidate parent; selecting candidate parents with uniform and robust body types as parent fishes; carrying out spawning induction on the male fish and the female fish of the Dongstellate star; collecting ova and sperms for artificial insemination; uniformly putting the fertilized eggs into an indoor nursery pond for hatching and breeding. The euryale ferox bred through the method is high in growth and development speed, high in muscle fat content, high in crude protein content, high in flavor amino acid content, delicious in taste, more popular with consumers and good in market prospect.

The invention discloses an application of an NtGS2 gene in increasing amino acid and chlorophyll content of tobacco leaves. According to the research, two GS2 family genes NtGS2-1 and NtGS2-2 are cloned from a tobacco cultivated variety K326; the glutamine synthetase activities of NtGS2-1 and NtGS2-2 overexpressed plant leaves are respectively increased by 41% and 13% compared with those of a control group, and the total amino acid contents are respectively 1.46 times and 1.93 times of those of a wild type. NtGS2-1 and NtGS2-2 play an important role in amino acid metabolism of tobacco, and besides glutamine, the NtGS2-1 and NtGS2-2 can promote synthesis of various other amino acids, which shows that the NtGS2-1 and NtGS2-2 have great application potential in tobacco quality improvement and production.

The invention discloses a non-fried instant noodle formula capable of improving amino acid score and a preparation method thereof, and the formula comprises the following raw materials in percentage by mass: 40% of chickpea powder, 8% of vital gluten, 44% of wheat flour, 8% of foreign protein, 1% of salt and 30% of distilled water. Wherein the foreign protein is one or a composition of egg white protein and soybean protein isolate. The protein content, the essential amino acid content and the protein digestibility are effectively improved, and the amino acid score is corrected. When the addition amount of the two exogenous high-quality proteins is 2% of egg white protein and 6% of soybean protein isolate, compared with a blank sample, the content of protein in a group added with the high-quality proteins is increased by 136.24%, the content of essential amino acid is increased by 56.85%, and the PDCAAS value is increased by 72.09%.

The application discloses a high-sweetness-amino-acid-content Flammulina velutipes variety and a MNP molecular identification method and application thereof. The Flammulina velutipes variety has excellent properties, the cap is not easy to open, the content of sweet amino acids is high, the diversified market demand is met, the factory annual bottle cultivation is suitable, and the Flammulina velutipes variety has a good application and popularization prospect. The MNP molecular fingerprint of the Flammulina velutipes variety 'Shangyan A111' is comprehensive multiple amplification and sequencing technology, sequence analysis of all marker sites of multiple samples can be performed at one time, compared with ISSR, RAPD, SSR and other molecular markers and mushrooming test, the MNP molecular fingerprint has the advantages of high throughput, multi-target, high sensitivity and high precision. The MNP molecular fingerprint of the Flammulina velutipes variety 'Shangyan A111' has specificity and specificity in identifying the Flammulina velutipes strain 'Shangyan A111'.

The invention belongs to the technical field of quality detection of traditional Chinese medicinal materials, and particularly relates to a method for determining the content of amino acid in periplaneta americana. The method comprises the following steps: a, preparation of a test solution: taking American cockroach medicinal material powder, adding water, carrying out ultrasonic treatment, carrying out solid-liquid separation to obtain a separation solution, enabling the separation solution to pass through a cation exchange resin column, soaking, eluting, collecting the eluent, drying by distillation, adding a hydrochloric acid solution, dissolving, filtering, and taking a subsequent filtrate to obtain the test solution; b, preparing a reference substance solution; and c, determination: respectively and precisely sucking the reference substance solution and the test solution, injecting into a high performance liquid chromatograph, determining, and calculating the content by using an external standard two-point method logarithmic equation. The method provided by the invention has good linearity, repeatability, stability and recovery rate, and can effectively monitor the content of various amino acids in periplaneta americana.

The strains and methods of the present invention relate to yeast cells that overproduce selected endogenous proteins having a high amino acid content of selected amino acids. The yeast can be used in conventional bioethanol production facilities to produce alcohol and increased amounts of selected amino acids, thereby improving the quality and commercial value of fermentation products and co-products such as animal feed ingredients.