256 results about "Fish skin" patented technology

The invention particularly relates to a method for extracting ACE (Angiotensin Converting Enzyme) inhibitory peptide from tilapia skin. The tilapia skin is a by-product rich in collagen, and has important development significance as a screening raw material of the ACE inhibitor. The invention provides a method for extracting an ACE (Angiotensin Converting Enzyme) inhibitor by taking tilapia skin as a raw material, which comprises the following steps of: degreasing the tilapia skin, crushing, adding acid liquor, extracting to obtain fish skin collagen crude protein, adding enzyme into the fish skin collagen crude protein for enzymolysis, and reserving the part with the molecular weight of less than 3kDa in an enzymolysis product, namely the ACE inhibitory peptide. Particularly, the enzyme is a product of bacillus subtilis or bacillus coagulans under gelatin-induced fermentation.

Provided is a bionic rescue robot fish, including a fish body skeleton, a fish head skeleton, a fishtail swing mechanism, a pectoral fin swing mechanism, a rescue mechanism and a buoyancy adjusting mechanism. The fish body skeleton is movably connected to the fish head skeleton. The fishtail swing mechanism, the pectoral fin swing mechanism, the rescue mechanism and the buoyancy adjusting mechanism are arranged on the fish head skeleton. Bionic fish skin is attached to surfaces of the fish body skeleton and the fish head skeleton in a sealing manner. The fishtail swing mechanism, the pectoral fin swing mechanism, the rescue mechanism and the buoyancy adjusting mechanism are each communicated with a control unit. The rescue mechanism can release a lifesaving airbag, and the buoyancy adjusting mechanism can change the volume of fish belly part.

The present invention discloses a pectoral fin steering bionic fish driven by a dual-half-gear mechanism. The head of the fish comprises an outer shell formed by two interlocking shells, which is an asymmetrical structure with a waterproof cavity formed inside. Pectoral fins are arranged on either side of the outer shell, and a pectoral fin control mechanism for controlling the rotation of the pectoral fins is located within the waterproof cavity. The tail is formed by assembling a multi-section skeleton. A dual-half-gear tail swing mechanism for controlling the swing of the tail fin is located within the skeleton. The skeleton is covered with waterproof fish skin. The dual-half-gear tail swing mechanism includes a main shaft, two spaced-apart half-gears connected to the main shaft, and a rocker arm. The rocker arm has a mating gear, which is connected to a sleeve arm that is inserted into the rocker arm to form a linear bearing structure. The sleeve arm extends rearwardly through a pin to connect to the skeleton. The dual-half-gear mechanism and the linear bearing structure transmit swinging power to control the swing of the fish tail. The present invention utilizes a dual-half-gear tail swing mechanism to control tail swinging, resulting in high mechanical transmission efficiency, high control accuracy, and the ability to perform relatively complex motions.

The invention discloses a preparation method and application of anti-photoaging collagen polypeptide. The preparation method comprises the following steps: unfreezing and cleaning silver carp skin, airing and cutting; naHCO3 is added, and soaking is carried out; adding Na2CO3 and carrying out ultrasonic impurity removal; performing ultrasonic-assisted treatment; adding protease for enzymolysis treatment; establishing an enzymolysis kinetic model under the alkaline protease condition; standing, cooling and centrifuging the enzymatic hydrolysate, collecting supernate, filtering, and performing vacuum freeze drying to obtain faint yellow SCSCP; the light aging resistant peptide SCSCP02 is separated, and the active peptide sequences of the light aging resistant peptide SCSCP02 are GPPGPPGTPGPQ, SGLPGPIGPPGPR and GLPGPIGPPGPR. The active substances which are extracted from the fish skin and have the anti-photoaging effect can reduce the risk of skin light damage caused by UVR by acting on various treatment targets, resource waste can be reduced, environmental pollution caused by biological resources can be relieved, and sustainable development of the industry can be promoted.

The invention relates to the technical field of collagen peptide extraction, and particularly discloses aquatic collagen tripeptide and a processing technology thereof, and the processing technology comprises the following steps: step S1, fish skin pretreatment; step S2, performing enzymolysis; step S3, fermentation; step S4, separation and purification; starting from three aspects, firstly, a modified degreasing agent is added in the degreasing treatment process, the modified degreasing agent takes 1-ethyl-3-methylimidazole acetate, zinc phytate and calcium citrate as main components according to a specific mass ratio, and secondly, gel emulsion is added in the enzymolysis process, so that the modified degreasing agent is prepared. The gel emulsion has an oil gel structure, grape seed oil and sodium lignin sulfonate, and 3, a composite strain is added in the fermentation process, the composite strain is prepared by mixing bacillus natto and lactobacillus plantarum according to a specific proportion, and the purity and the extraction rate of the aquatic collagen tripeptide are improved through the synergistic effect of all the procedures.

The invention provides a fish collagen peptide with skin brightening and freckle removing effects as well as a preparation method and application thereof, and belongs to the technical field of protein peptides. The preparation method comprises the following steps: by taking fish skin and / or fish scales as raw materials, performing degreasing and fishy smell removal treatment, and then sequentially performing heat treatment, homogenization treatment, enzymolysis treatment and enzyme deactivation refining to obtain the fish collagen peptide. In the fish collagen peptide prepared by the invention, the proportion of polypeptide with the molecular weight of 189-3000Da is more than 90%, and the protein content is more than 99.23%. Experiments show that the fish collagen peptide can effectively reduce the skin color spot area proportion of a subject and improve the skin L value and ITA value of the subject at the same time. Therefore, the fish collagen peptide prepared by the invention has good skin brightening and freckle removing effects, and a new way is provided for preparing related products.

The present invention specifically relates to a method for extracting ACE inhibitory peptides from tilapia skin. Tilapia skin is a byproduct rich in collagen, and has important development significance as a raw material for screening ACE inhibitors. The present invention is designed to provide a method for extracting ACE inhibitors using tilapia skin as a raw material, comprising the following steps: defatting the tilapia skin and crushing it, adding acid to extract to obtain fish skin collagen crude protein, adding enzyme to the fish skin collagen crude protein for enzymolysis, and retaining the part of the enzymolysis product with a molecular weight less than 3kDa is the above-mentioned ACE inhibitory peptide; in particular, the above-mentioned enzyme is a product of Bacillus subtilis or Bacillus coagulans under gelatin-induced fermentation.

The invention belongs to the technical field of collagen peptide extraction, and discloses a preparation process of low-fishy-smell marine fish skin collagen peptide, which is used for solving the problems of incomplete fishy smell removal and high protein loss rate in the traditional fishy smell removal process. Comprising the following steps: (1) immersing fish skin into water, adding lipase, cleaning the fish skin with pure water, and homogenizing to obtain homogenate; (2) adding saccharomyces cerevisiae and purple perilla juice into the homogenate, carrying out heat preservation and enzymolysis at 30 DEG C, then heating to 45 DEG C, adding compound enzyme for enzymolysis, and heating for enzyme deactivation to obtain collagen peptide enzymatic hydrolysate; (3) adding activated carbon and diatomite into the collagen peptide enzymatic hydrolysate, keeping the temperature at 70 DEG C, stirring, and carrying out filter pressing and refined filtration to obtain a collagen peptide decoloring solution; (4) sterilizing and concentrating the collagen peptide decoloring solution to obtain a collagen peptide concentrated solution; and (5) drying the collagen peptide concentrated solution to obtain collagen peptide powder. The collagen peptide prepared by the method is almost free of fishy smell and low in protein loss.

The present invention relates to the technical field of active substance extraction, and specifically relates to a mineral-containing high-purity collagen tripeptide and a preparation method therefor. A high-purity collagen tripeptide containing mineral elements and having a high hydroxyproline content and no bad flavor, particularly no bitterness is obtained by using a byproduct of aquatic product processing, i.e., fish skin or fish scales, as a raw material, using a one-step enzymolysis method, and performing nanofiltration twice.

The invention provides collagen tripeptide liquid with moisturizing and anti-photoaging effects and a preparation method thereof, and relates to the technical field of bioactive peptides.The preparation method comprises the steps that pretreatment and first-time enzymolysis are conducted, specifically, pretreated fish skin and water are mixed, the pH value is adjusted, compound protease is added for enzymolysis, and first enzymatic hydrolysate is obtained; second-time enzymolysis: adjusting the pH value of the first enzymatic hydrolysate to 6.0-7.5, adding neutral protease for enzymolysis, and then adding aminopeptidase for enzymolysis for 20-40 minutes to obtain second enzymatic hydrolysate; and performing enzyme deactivation, decoloration, separation and purification to obtain collagen tripeptide liquid. Wherein the content of collagen tripeptide, the content of glycine-proline-hydroxyproline and the content of hydroxyproline are high, and good moisturizing and anti-photoaging effects are achieved.

Collagen peptide compositions and methods for producing these collagen peptide compositions from fish skin or fish scale are provided. The collagen peptide compositions are characterized by high protein content, minimal impurities, enriched hydroxyproline, and specific peptide sequences, making them suitable for use as supplements or additives in food and beverages.

The invention discloses a collagenase mutant, a gene segment, a recombinant plasmid, a recombinant expression system and application thereof, and a method for preparing the collagenase mutant and collagen tripeptide, and belongs to the field of gene engineering and fermentation engineering. The invention provides a collagenase mutant KT231 capable of realizing high-efficiency secretory expression in streptomyces S.lividans TK24 and a preparation method of the collagenase mutant KT231. The collagenase mutant KT231 can be used for efficiently carrying out enzymolysis on the skin of the narrow gadus at 60 DEG C, has excellent heat resistance and higher enzyme activity, and can be used for efficiently preparing the collagen tripeptide from the skin of the narrow gadus. The collagen peptide is compounded with commercially available papaya chymotrypsin, so that the proportion of collagen tripeptide in the fish skin enzymatic hydrolysate can be remarkably increased, and the fish skin enzymatic hydrolysate has no bitter taste. The method has wide application prospects in preparation of products, tissue engineering materials and wound healing accelerators for resisting wrinkles, preserving moisture, repairing damaged skin and improving bone and joint health.

The preparation method comprises the following steps: (1) fish skin pretreatment: (2) gas foaming treatment: weighing sodium borohydride with a certain molar concentration, dissolving the sodium borohydride in a phosphate buffer solution with a certain concentration, stirring until bubbles in the solution are obviously generated and most of solids are dissolved, and then, carrying out gas foaming treatment; putting the cleaned fish skin tissues in the step (2), and performing foaming treatment; (3) cleaning treatment; and (4) protection and storage. The gas foaming technology is innovatively adopted for decellularization treatment, the interface stripping effect between cells and a matrix is remarkably enhanced on the premise that the collagen three-dimensional structure and the scaffold morphology integrity are not damaged, and the method is suitable for the decellularization pretreatment process of soft tissue (such as fish skin and placenta) with a compact structure or cell-intensive type. The development of a biomedical material, especially a tissue engineering scaffold technology, is promoted, and the preparation method has a wide application prospect.

The invention discloses a preparation method and application of a fish skin albumen glue edible film, and belongs to the technical field of food film packaging and preparation.The method comprises the steps that a fish skin albumen glue solution and a low-acyl gellan gum solution are mixed, first homogenization treatment, high-pressure microjet treatment and spray drying treatment are sequentially conducted, and a fish skin albumen glue mixture is obtained; dissolving in water, adding glycerol and stirring to obtain a mixed solution; and after ultrasonic defoaming treatment, putting into a mold, drying and demolding to obtain the fish skin albumen glue edible film. When the fish skin albumen glue prepared by the preparation method provided by the invention is applied to the manufacturing process of the film, the safety performance of the film can be effectively improved, the advantage of degradability is beneficial to environmental protection, and in addition, the film produced by adopting the fish skin albumen glue has the advantages of degradability, edibility, good mechanical property, high safety and the like.

The invention relates to a preparation method and application of collagen tripeptide liquid, and belongs to the technical field of bioactive peptides, the preparation method comprises the following steps: A1, pretreating fish skin raw materials to obtain fish skin homogenate; a2, the pH value of the fish skin homogenate is adjusted to be neutral, compound protease accounting for 1-4% of the mass of the fish skin is added for enzymolysis, and a collagen peptide crude extracting solution is obtained; a3, performing enzyme deactivation and centrifugation, collecting supernate, and performing nanofiltration to obtain a filtered component 1 and an intercepted component 1; a4, putting the interception component 1 into a hydrochloric acid solution, and hydrolyzing to obtain hydrolysate; adjusting the hydrolysate to be neutral, and then carrying out nanofiltration to obtain a filtered component 2 and an intercepted component 2; and A5, mixing the filtered component 1 and the filtered component 2, carrying out nanofiltration, and collecting an intercepted component 3 to obtain a collagen tripeptide solution. The collagen tripeptide in the prepared collagen tripeptide liquid is high in yield, and the purity is also effectively improved.

The invention provides a preparation method of a double-layer guided tissue regeneration membrane, the double-layer guided tissue regeneration membrane and application of the double-layer guided tissue regeneration membrane, and relates to the field of biomedical materials. The preparation method comprises the following steps: carrying out enzymolysis treatment on pretreated fish skin by combining an acid method with a restrictive enzymolysis method to obtain telopeptide-removed collagen; carrying out electrostatic spinning on the blended spinning solution of the telopeptide-removed collagen and the polylactic acid to form a loose layer, and carrying out electrostatic spinning on the loose layer by adopting the polylactic acid spinning solution to form a compact layer, so as to obtain a double-layer composite membrane; and crosslinking the double-layer composite membrane to obtain the double-layer guided tissue regeneration membrane. The double-layer guided tissue regeneration membrane has low immunogenicity, good mechanical properties and excellent histocompatibility, the compact layer can play a barrier role and prevent fibroblasts from occupying an osteogenic space, the loose surface can promote cell adhesion and growth and accelerate bone tissue repair, and the interface of the double-layer structure is tightly combined.

The invention discloses a preparation method of composite active peptide with anti-wrinkle, whitening and freckle-removing effects from marine fish byproducts, cod byproducts, namely fish heads, fish bones and fish skins, are used as raw materials and are mixed according to the mass ratio of 3: 3: 4, and the composite active peptide is prepared through the steps of pretreatment, enzymolysis, enzyme deactivation, centrifugal separation and purification. A compound enzyme used in the enzymolysis step is composed of trypsin and papain according to a certain proportion, and the enzyme addition amount, the enzymolysis time, the enzymolysis temperature and other conditions are optimized. The prepared composite active peptide with the anti-wrinkle, whitening and freckle-removing effects from the marine fish by-product has good anti-wrinkle, whitening and freckle-removing effects, resource utilization of the cod by-product is achieved, and the composite active peptide has high application value.

The invention relates to the technical field of biology, in particular to antioxidative peptide and ACE (angiotensin converting enzyme) inhibitory peptide from tuna as well as a preparation method and application of the antioxidative peptide and the ACE inhibitory peptide. The amino acid sequences of the polypeptide provided by the invention are as shown in SEQ ID NO.1-6. The preparation method comprises the following steps: by taking processing waste tuna skin as a raw material, carrying out enzymolysis to obtain tuna skin collagen, then carrying out liquid chromatography-tandem mass spectrometry identification and molecular docking screening on a tuna skin collagen zymolyte, and extracting to obtain polypeptide with antioxidant activity and polypeptide capable of effectively inhibiting ACE (Angiotensin Converting Enzyme) activity. According to the method, the protein extraction step is simplified; meanwhile, on one hand, the deep processing level of the tuna industry can be improved, high-value utilization is achieved, and on the other hand, the bearing pressure of the environment on the processing waste can be reduced.

The invention relates to the technical field of cosmetics, and aims to provide a micromolecular protein peptide for enhancing transdermal absorption, which is prepared by the following steps: cleaning a salmon skin raw material, and performing water extraction to obtain a salmon collagen stock solution; papain is added into the salmon collagen stock solution for primary enzymolysis, carboxypeptidase B is added for secondary enzymolysis, enzyme deactivation is performed, and a salmon collagen peptide solution is obtained; and sequentially carrying out centrifugation and ultrafiltration treatment on the salmon collagen peptide solution, and freeze-drying to obtain the micromolecular protein peptide for enhancing transdermal absorption. According to the preparation method disclosed by the invention, the salmon collagen is decomposed into larger peptide fragments by adopting papain, and then the peptide fragments are further hydrolyzed by using carboxypeptidase B, so that the extracted salmon collagen peptide is low in bitter taste degree and has high transdermal absorbability through cooperative use of the papain and the carboxypeptidase B.

A rope-driven X-shaped parallel structure tensegrity flexible bionic fish, relating to a bionic fish. An MCU controller, a battery and a servo motor are installed in the fish head shell, a waterproof switch is installed on the top to control the MCU controller, a servo motor fixes a swing plate, a first fish body joint, a second fish body joint, a third fish body joint and a fish tail joint are arranged in sequence behind the fish head shell and wrapped with bionic fish skin, the first fish body joint and the fish tail joint are connected by two springs, the first fish body joint and the second fish body joint, the second fish body joint and the third fish body joint, and the third fish body joint and the fish tail joint are respectively hinged by an upper connecting rod and a lower connecting rod, the corresponding upper connecting rod and the lower connecting rod are arranged in an X shape, the swing plate is connected to the fish tail joint by two active drive ropes, and the tail fin is installed at the rear end of the fish tail joint through a tension joint. The shortcomings of the hinge mechanism with low rotational stiffness and inability to absorb lateral impact loads are solved, so that the swimming process of the bionic fish is more realistic and stable.

The invention relates to the technical field of mixing equipment, and discloses a stuffing mixing device for fish-skin mashed shrimp processing, which comprises a mixing machine body, a bottom plate for supporting is mounted at the bottom of the mixing machine body, a motor box is mounted at the top of the mixing machine body, a motor for driving rotation is mounted in the motor box, and the mixing machine body is internally provided with a motor for driving rotation. A mixing barrel for placing materials is mounted at the bottom of the outer wall of the mixing machine body, and a stirring assembly for mixing the materials is mounted at the output end of the motor; the stirring assembly comprises a rotating disc, a rotating shaft, stirring rods and stirring blades, the rotating disc is mounted at the output end of the motor, and the rotating shaft is eccentrically mounted at the bottom of the rotating disc. By arranging the control assembly and the adjusting assembly, the distance and the angle of the stirring blades can be adjusted according to the pressure of material extrusion on the stirring blades, and the material mixing effect is improved.

The invention provides a fishy smell-free DHA (docosahexaenoic acid) small dropping pill and a preparation method thereof, the fishy smell-free DHA small dropping pill comprises: a capsule skin, the capsule skin is prepared from a gelatin solution comprising gelatin, glycerin and water, the capsule skin presents a frosted appearance, and the gelatin comprises mixed pigskin gelatin and fish skin gelatin; the DHA dropping pill comprises a capsule skin and a content wrapped in the capsule skin, the content comprises grease and N-acetylneuraminic acid, the grease at least comprises DHA algal oil, and the DHA dropping pill prepared by the scheme is not prone to oxidative deterioration under illumination, free of fishy smell and not prone to adhesion under high temperature or high humidity.

The invention belongs to the technical field of biological medicine manufacturing, and particularly relates to preparation and application of collagen peptide with whitening activity. The preparation method of the collagen peptide with the whitening activity comprises the following steps: step A, inoculating bacillus subtilis into a culture medium containing a moringa oleifera leaf extracting solution, fermenting for 6-12 hours, inoculating aspergillus niger, continuously fermenting for 36-48 hours, sterilizing, centrifuging, and taking supernate to obtain moringa oleifera leaf fermentation liquor; and step B, scaling the fish skin, cleaning, drying and crushing, adding the moringa oleifera leaf fermentation liquor, reacting for 6-10 hours at the pH value of 6.0-7.0 and the temperature of 35-45 DEG C, and performing ultrafiltration, filtrate concentration and freeze-drying to obtain the collagen peptide. The preparation process has the advantages of low cost, greenness and high efficiency, and the prepared collagen peptide shows the activity of inhibiting tyrosinase and can be applied to preparation of skin care products or medicines with a whitening function.

The utility model discloses a conveying assembly for fish processing, which comprises symmetrically arranged supporting frames, conveying box bodies are arranged on the supporting frames, one conveying box body is fixed on the supporting frame on the right side, a supporting seat is arranged on one side of each conveying box body, and conveying belt parts are symmetrically arranged in the conveying box bodies. The front side and the rear side of each conveying box body are provided with elastic parts which can elastically extrude the conveying belt parts which are symmetrically arranged up and down, each supporting seat is provided with a peeling part, the rear side of each supporting seat is provided with a driving mechanism, and the other supporting frame is provided with a rotating mechanism which can drive the conveying box body to rotate. Compared with the prior art, the fish skin can be automatically removed, the working efficiency is improved, and meanwhile fishes of different sizes can be treated respectively.

The invention provides an application of a fish skin gelatin composite coating as a preservative in aquatic products, the aquatic products are oysters, the fish skin gelatin composite coating is an aqueous solution containing 1-1.5 g of fish skin gelatin and 20-30 mg of UVC-GA, and the fish skin gelatin composite coating is prepared by dissolving fish skin gelatin freeze-dried powder in water, adding a proper amount of a preservative, and uniformly stirring. The preparation method comprises the following steps: preparing a fish skin gelatin solution with the volume fraction of 5%, adding a UVC-GA antibacterial solution with the volume fraction of 0.2%, uniformly stirring, adjusting the pH value of the mixed solution to 5.5-6.5, placing the obtained mixed solution in a water bath with the temperature of 55 DEG C, keeping the temperature for 35 minutes, and carrying out ultrasonic treatment for 25 minutes for defoaming. The method effectively prolongs the shelf life of the oysters, provides a new method for aquatic products, is a promising method for delaying the deterioration of the quality of the aquatic products and reducing the loss after capturing, and has huge application potential.

The invention discloses an animal feed additive composition, an animal feed and application of the animal feed. The animal feed additive comprises marine animal-derived hydroxyapatite, takifugu fish skin glue, composite algae powder and euphausia superba powder. Marine animal source hydroxyapatite is derived from takifugu calthrop bone meal. The animal feed additive composition comprises the following components in percentage by mass: 40%-50% of takifugu calthrop bone meal; 10%-15% of takifugu fish skin glue; 30%-40% of composite algae powder; and 10%-15% of euphausia superba powder. The invention also discloses an animal feed, which comprises a basal feed and the animal feed additive composition accounting for 3.0-6.0% of the mass of the basal feed. According to the application, the takifugu calthrops are converted into the animal feed additive composition with high additional value from fish skin processing waste for the first time, so that single-component chemical additives such as stone powder and calcium hydrophosphate which are widely used at present can be replaced, and resource utilization and high-value utilization of marine fishery waste are realized.

The utility model discloses a skinning machine which comprises a main body, the main body is provided with a tool apron frame with a blade, a soft roller, a tooth roller and a scraping comb plate, the soft roller and the tooth roller are rotatably arranged on the main body, the scraping comb plate is fixedly connected to the lower portion of the tooth roller, a working wheel is arranged between the soft roller and the tooth roller, and the working wheel is fixedly connected with the tooth roller. And a water spraying device is also arranged below the main body. The added water spraying device can flush away fascia remaining on the knife edge, so that the knife edge is kept sharp, the fish skin can be completely peeled, the surface of the peeled fish can be smooth, meanwhile, the knife holder and the fish can be lubricated by sprayed water, and the fish can be smoothly moved and conveyed.

The invention relates to the technical field of collagen peptide extraction, and specifically discloses an aquatic collagen tripeptide and a processing technology thereof. The process comprises the following steps: step S1, pretreating fish skin; step S2, enzymatic hydrolysis; step S3, fermentation; and step S4, separation and purification. The invention proceeds from three aspects: first, a modified degreasing agent is added during the degreasing process, wherein the modified degreasing agent comprises 1-ethyl-3-methylimidazole acetate, zinc phytate and calcium citrate in a specific mass ratio as main components; second, a gel emulsion is added during the enzymatic hydrolysis process, wherein the gel emulsion has an oil gel structure, grape seed oil and sodium lignin sulfonate; and third, a composite strain is added during the fermentation process, wherein the composite strain is prepared by mixing Bacillus natto and Lactobacillus plantarum in a specific ratio. Through the synergistic effect of the above steps, the purity and extraction rate of the aquatic collagen tripeptide are improved.

The invention belongs to the technical field of nutrient solutions, and particularly relates to a concentrated nutrient solution helpful for improving special nutritional ingredients of animals and having health-care efficacy, the concentrated nutrient solution is prepared from the following components: composite small molecule peptides, composite vitamins, composite minerals, composite amino acids and fructo-oligosaccharide; wherein the composite small molecule peptide is formed by mixing fish skin oligopeptide, sea cucumber peptide and corn oligopeptide; the compound vitamin is formed by mixing vitamin A, vitamin B12, vitamin C and vitamin D3; the composite mineral substance is formed by mixing calcium lactate, calcium hydrophosphate, ferrous sulfate and zinc sulfate; the compound amino acid is formed by mixing lysine, methionine, threonine and tryptophan. The fish skin oligopeptide, the sea cucumber oligopeptide and the corn oligopeptide in the composite small molecule peptide can achieve a synergistic effect, can promote growth of young broilers, can promote nutrient absorption and can reduce feed consumption; in addition, the immunity of the chick broiler chicken can be improved, so that the survival rate is increased.

The present application belongs to the technical field of nutritional functional food and health food, and particularly relates to a preparation method of marine fish skin collagen oligopeptide beneficial to promoting skin health, and a product and application thereof. The method comprises the following steps: after deep sea fish skin is pretreated by salt dissolution with a NaCl solution, trisodium citrate is added as an enzymolysis aid, and neutral protease (from Trichoderma reesei) and papain are sequentially used for two-step enzymolysis, and after enzyme inactivation, impurities are removed, sterilization is performed, and concentration, drying are performed to obtain the product. In vitro cell experiment verification shows that the product can significantly promote human skin fibroblast proliferation; in vivo animal experiment verification shows that the product can significantly increase skin moisture content, reduce transdermal water loss, increase hydroxyproline content, improve SOD activity and reduce MDA content. The product is suitable for the field of nutritional functional food and health food, and has a good application prospect in promoting skin health.