138 results about "Fish skin" patented technology

The preparation method comprises the following steps: (1) fish skin pretreatment: (2) gas foaming treatment: weighing sodium borohydride with a certain molar concentration, dissolving the sodium borohydride in a phosphate buffer solution with a certain concentration, stirring until bubbles in the solution are obviously generated and most of solids are dissolved, and then, carrying out gas foaming treatment; putting the cleaned fish skin tissues in the step (2), and performing foaming treatment; (3) cleaning treatment; and (4) protection and storage. The gas foaming technology is innovatively adopted for decellularization treatment, the interface stripping effect between cells and a matrix is remarkably enhanced on the premise that the collagen three-dimensional structure and the scaffold morphology integrity are not damaged, and the method is suitable for the decellularization pretreatment process of soft tissue (such as fish skin and placenta) with a compact structure or cell-intensive type. The development of a biomedical material, especially a tissue engineering scaffold technology, is promoted, and the preparation method has a wide application prospect.

The invention discloses a preparation method of composite active peptide with anti-wrinkle, whitening and freckle-removing effects from marine fish byproducts, cod byproducts, namely fish heads, fish bones and fish skins, are used as raw materials and are mixed according to the mass ratio of 3: 3: 4, and the composite active peptide is prepared through the steps of pretreatment, enzymolysis, enzyme deactivation, centrifugal separation and purification. A compound enzyme used in the enzymolysis step is composed of trypsin and papain according to a certain proportion, and the enzyme addition amount, the enzymolysis time, the enzymolysis temperature and other conditions are optimized. The prepared composite active peptide with the anti-wrinkle, whitening and freckle-removing effects from the marine fish by-product has good anti-wrinkle, whitening and freckle-removing effects, resource utilization of the cod by-product is achieved, and the composite active peptide has high application value.

The invention relates to the technical field of mixing equipment, and discloses a stuffing mixing device for fish-skin mashed shrimp processing, which comprises a mixing machine body, a bottom plate for supporting is mounted at the bottom of the mixing machine body, a motor box is mounted at the top of the mixing machine body, a motor for driving rotation is mounted in the motor box, and the mixing machine body is internally provided with a motor for driving rotation. A mixing barrel for placing materials is mounted at the bottom of the outer wall of the mixing machine body, and a stirring assembly for mixing the materials is mounted at the output end of the motor; the stirring assembly comprises a rotating disc, a rotating shaft, stirring rods and stirring blades, the rotating disc is mounted at the output end of the motor, and the rotating shaft is eccentrically mounted at the bottom of the rotating disc. By arranging the control assembly and the adjusting assembly, the distance and the angle of the stirring blades can be adjusted according to the pressure of material extrusion on the stirring blades, and the material mixing effect is improved.

The invention belongs to the technical field of biological medicine manufacturing, and particularly relates to preparation and application of collagen peptide with whitening activity. The preparation method of the collagen peptide with the whitening activity comprises the following steps: step A, inoculating bacillus subtilis into a culture medium containing a moringa oleifera leaf extracting solution, fermenting for 6-12 hours, inoculating aspergillus niger, continuously fermenting for 36-48 hours, sterilizing, centrifuging, and taking supernate to obtain moringa oleifera leaf fermentation liquor; and step B, scaling the fish skin, cleaning, drying and crushing, adding the moringa oleifera leaf fermentation liquor, reacting for 6-10 hours at the pH value of 6.0-7.0 and the temperature of 35-45 DEG C, and performing ultrafiltration, filtrate concentration and freeze-drying to obtain the collagen peptide. The preparation process has the advantages of low cost, greenness and high efficiency, and the prepared collagen peptide shows the activity of inhibiting tyrosinase and can be applied to preparation of skin care products or medicines with a whitening function.

The utility model discloses a conveying assembly for fish processing, which comprises symmetrically arranged supporting frames, conveying box bodies are arranged on the supporting frames, one conveying box body is fixed on the supporting frame on the right side, a supporting seat is arranged on one side of each conveying box body, and conveying belt parts are symmetrically arranged in the conveying box bodies. The front side and the rear side of each conveying box body are provided with elastic parts which can elastically extrude the conveying belt parts which are symmetrically arranged up and down, each supporting seat is provided with a peeling part, the rear side of each supporting seat is provided with a driving mechanism, and the other supporting frame is provided with a rotating mechanism which can drive the conveying box body to rotate. Compared with the prior art, the fish skin can be automatically removed, the working efficiency is improved, and meanwhile fishes of different sizes can be treated respectively.

The invention discloses an animal feed additive composition, an animal feed and application of the animal feed. The animal feed additive comprises marine animal-derived hydroxyapatite, takifugu fish skin glue, composite algae powder and euphausia superba powder. Marine animal source hydroxyapatite is derived from takifugu calthrop bone meal. The animal feed additive composition comprises the following components in percentage by mass: 40%-50% of takifugu calthrop bone meal; 10%-15% of takifugu fish skin glue; 30%-40% of composite algae powder; and 10%-15% of euphausia superba powder. The invention also discloses an animal feed, which comprises a basal feed and the animal feed additive composition accounting for 3.0-6.0% of the mass of the basal feed. According to the application, the takifugu calthrops are converted into the animal feed additive composition with high additional value from fish skin processing waste for the first time, so that single-component chemical additives such as stone powder and calcium hydrophosphate which are widely used at present can be replaced, and resource utilization and high-value utilization of marine fishery waste are realized.

The invention belongs to the technical field of nutrient solutions, and particularly relates to a concentrated nutrient solution helpful for improving special nutritional ingredients of animals and having health-care efficacy, the concentrated nutrient solution is prepared from the following components: composite small molecule peptides, composite vitamins, composite minerals, composite amino acids and fructo-oligosaccharide; wherein the composite small molecule peptide is formed by mixing fish skin oligopeptide, sea cucumber peptide and corn oligopeptide; the compound vitamin is formed by mixing vitamin A, vitamin B12, vitamin C and vitamin D3; the composite mineral substance is formed by mixing calcium lactate, calcium hydrophosphate, ferrous sulfate and zinc sulfate; the compound amino acid is formed by mixing lysine, methionine, threonine and tryptophan. The fish skin oligopeptide, the sea cucumber oligopeptide and the corn oligopeptide in the composite small molecule peptide can achieve a synergistic effect, can promote growth of young broilers, can promote nutrient absorption and can reduce feed consumption; in addition, the immunity of the chick broiler chicken can be improved, so that the survival rate is increased.

The present application belongs to the technical field of nutritional functional food and health food, and particularly relates to a preparation method of marine fish skin collagen oligopeptide beneficial to promoting skin health, and a product and application thereof. The method comprises the following steps: after deep sea fish skin is pretreated by salt dissolution with a NaCl solution, trisodium citrate is added as an enzymolysis aid, and neutral protease (from Trichoderma reesei) and papain are sequentially used for two-step enzymolysis, and after enzyme inactivation, impurities are removed, sterilization is performed, and concentration, drying are performed to obtain the product. In vitro cell experiment verification shows that the product can significantly promote human skin fibroblast proliferation; in vivo animal experiment verification shows that the product can significantly increase skin moisture content, reduce transdermal water loss, increase hydroxyproline content, improve SOD activity and reduce MDA content. The product is suitable for the field of nutritional functional food and health food, and has a good application prospect in promoting skin health.

The present application relates to the technical field of biology, and particularly relates to an antioxidant peptide and ACE inhibitory peptide from tuna, and a preparation method and application thereof. The amino acid sequence of the polypeptide provided by the present application is shown as SEQ ID NO. 1-6. The present application takes tuna skin waste as raw material, obtains tuna skin collagen through enzymolysis, then performs liquid chromatography-tandem mass spectrometry identification and molecular docking screening on the tuna skin collagen enzymolysis product, and obtains polypeptides with antioxidant activity and polypeptides effectively inhibiting ACE activity. The method simplifies the protein extraction step. Meanwhile, the method can improve the deep processing level of the tuna industry, achieve high-value utilization, and reduce the environmental pressure on processing waste.

The present application relates to the technical field of fishbone separating device, in particular to a fishbone separating machine for fish food processing, which comprises a base, the upper end of the base is fixedly connected with a processing cylinder, the outer side of the processing cylinder near the upper position is a feeding port, the inner side of the processing cylinder is provided with an extrusion roller shaft, the lower side of the extrusion roller shaft of the processing cylinder is fixedly connected with a filter cartridge, the inner side of the filter cartridge is installed with a separating mechanism for discharging minced meat, the inner side of the separating mechanism is installed with a fishbone removing mechanism for processing fishbone inside the filter hole of the filter cartridge, the hollow shaft and the installation cylinder are axially advanced by cooperating with the material removing assembly, the minced meat inside the filter cartridge can be stably and non-residually pushed outwards, which effectively avoids the decrease of separation efficiency caused by the accumulation of minced meat and the blockage of fishbone; the fishbone, fish skin and other waste materials outside the filter cartridge are synchronously cleaned by the driving arc-shaped scraper, forming a linkage operation mode of synchronous minced meat pushing and fishbone waste discharging.

The invention belongs to the technical field of collagen peptide preparation, and particularly relates to a proline aminopeptidase mutant and application thereof in collagen peptide preparation. The amino acid sequence of the proline aminopeptidase mutant is as shown in SEQ ID NO. 2. The proline aminopeptidase mutant provided by the invention has the advantages of high enzyme activity, wide temperature and pH adaptation range, capability of adapting to a long-time high-temperature environment of collagen enzymolysis and the like, and is suitable for deep hydrolysis of collagen. Through cooperation with neutral protease, papain and collagenase, collagen raw materials from fish skin can be effectively degraded, the yield of low-molecular-weight peptide is increased, and collagen peptide with the molecular weight smaller than 1000 Da and accounting for 90% or above, collagen tripeptide accounting for 39% or above, collagen dipeptide accounting for 2% or above and the content of characteristic dipeptide Hyp-Gly being remarkably increased is generated. The method has important application value in the field of oriented preparation of collagen dipeptide.

This invention relates to a pufferfish TRPV1 inhibitory peptide, its preparation method, and its application. The amino acid sequence of the peptide is LDIF. The preparation method includes: 1. Enzymatically hydrolyzing pufferfish skin, then inactivating the enzyme, and screening for peptides with a molecular weight not greater than 1 kDa from the hydrolysate, followed by freeze-drying to obtain the pufferfish skin enzymatic hydrolysate peptide; 2. Performing mass spectrometry analysis on the pufferfish skin enzymatic hydrolysate peptide, using mass spectrometry analysis software, and selecting multiple non-repeating peptide sequences based on the criteria of confidence level -10lgP > 20 and amino acid count < 10; 3. Molecularly docking the selected peptide sequences with the TRPV1 receptor using software to select peptide sequences with strong binding affinity to the TRPV1 receptor; 4. Solid-phase synthesis of the selected peptide sequences to obtain the peptide LDIF. This invention uses pufferfish skin as raw material to obtain a peptide with good inhibitory effect on TRPV1, which can be used to prepare skin care products for soothing sensitive skin.

This application belongs to the field of biotechnology, specifically relating to giant salamander skin collagen peptides, their preparation methods, and applications. This application provides a method for preparing giant salamander skin collagen peptides. The method first pre-treats the fish skin, then performs ultrasonic-assisted extraction under acidic conditions to effectively promote collagen dissolution. The extract undergoes flocculation to remove impurities, significantly reducing interference from other proteins. The enzymatic hydrolysis stage employs a stepwise enzymatic hydrolysis strategy: first, a combination of alkaline protease and another specific protease is used for hydrolysis, sequentially cleaving the complex structure of giant salamander skin collagen to improve initial hydrolysis efficiency and generate specific intermediate peptides; subsequently, a flavor protease is added for further deep hydrolysis to achieve complete hydrolysis. This method has high enzymatic hydrolysis efficiency, controllable hydrolysis degree, concentrated molecular weight distribution of the obtained collagen peptides, high yield of the target product, and stable process.

The application discloses a preparation method of sturgeon peptide and application of the sturgeon peptide in improving immunity and sexual function. The method comprises the following steps: S1, one or more of sturgeon cartilage, fish bone and fish skin are crushed after being thawed, and pure water is added according to a material-liquid ratio of 1:3-1:4 and is pumped into an enzyme hydrolysis tank; S2, the enzyme hydrolysis tank is heated to 85-90 DEG C under the condition of a closed low pressure of 0.06-0.08 MPa, and protein is denatured for 15-20 min; S3, the temperature is reduced to 60-65 DEG C, the pH is adjusted to 8.0-8.5, 2‰-3‰ alkaline protease of the raw material amount is added, and constant-temperature enzyme hydrolysis is carried out for 170-190 min; the temperature is increased to 85-90 DEG C after the enzyme hydrolysis is completed, and the enzyme is inactivated for 14-16 min; S4, decolorization is carried out; S5, filtration and concentration are carried out; S6, vacuum concentration is carried out; S7, sterilization and drying are carried out, and sturgeon peptide finished products are obtained. The sturgeon peptide obtained by the preparation method has small molecular weight and high active ingredients.

This invention relates to an efficient process designed to recycle materials from the food industry, preferably fisheries, such as materials extracted from fish skin, thereby enabling a universal, at least partially recyclable and sustainable process for consumer products, including bioplastic films.

This invention discloses an active peptide remover, method, and application for removing inappropriate decorations from the surface of stone cultural relics, relating to the field of stone cultural relic protection technology. The active peptide remover comprises: 0.5-5% surface active peptides, 0.1-1% auxiliary components, and the balance being deionized water; the surface active peptides are one or more selected from soybean peptides, deep-sea fish skin peptides, sea cucumber peptides, wheat peptides, corn peptides, silk fibroin peptides, and whey protein peptides; the auxiliary components are stabilizers and / or pH adjusters, the stabilizers being one or more selected from xanthan gum, carrageenan, bentonite, and kaolin, and the pH adjusters being citric acid or sodium hydroxide, with the active peptide remover having a pH value of 6.5-7.5. This active peptide remover not only avoids damage to stone cultural relics caused by high-temperature steam and sandblasting media; it also breaks down the adhesion between the decorations and the stone, helping to reduce the pressure, temperature, and time required for steam cleaning and sandblasting removal.

1. Name of the designed product: ornament (ice and snow fish skin picture). 2. Use of the designed product: the designed product is used for ornament and display. 3. Design points of the designed product: in shape. 4. Picture or photo showing the design points: perspective view.

The invention discloses energy-saving fish meat rapid separation equipment, and relates to the technical field of energy-saving aquatic product processing equipment.The separation equipment comprises a feeding bin, a meat separation barrel is arranged below the feeding bin, a meat separation belt is arranged below the meat separation barrel, and an adjusting mechanism used for adjusting the posture of fish meat is arranged in the feeding bin; through cooperation of a shifting plate and an auxiliary plate, the posture of fish meat fed into the feeding bin can be adjusted, so that the fish meat can enter in a horizontal posture when entering the meshing position of a meat separation barrel and a meat separation belt, the contact area of the fish meat with the meat separation barrel and the meat separation belt is increased, the fish meat is prevented from being wrapped by fish bones, fish skin and other impurities, and the meat separation efficiency is improved. According to the device, the posture of the fish meat is adjusted, the fish meat separation effect is influenced, secondary adjustment can be performed on the fish meat after posture adjustment through movement of the matching plate and the matching rod, part of the fish meat without posture adjustment can also be greatly adjusted, the requirement that the fish meat enters the meshing position of the meat separation barrel and the meat separation belt subsequently is met, and the fish meat separation effect is improved.

The invention discloses a special feeding method for sea farming of perches, and relates to the technical field of perch breeding and breeding, and the method comprises the following steps: S1, inner and outer support culture; s2, rotary feeding; s3, moving blocking; and S4, cleaning the feeding residual feed. After the movable sleeve is in contact with the culture box, the feeding area of the weever is temporarily closed relative to the side part of the culture box, so that the impact of seawater flow on feed is reduced, and the feed flowing out of the movable sleeve is reduced; therefore, attachments on the surface of the breeding box side net are rubbed and fall off, the cleanliness of the breeding box is improved, the time that the breeding box needs to be periodically pulled out of the water bottom to be cleaned and replaced is prolonged, and the situation that excessive algae are attached to the surface of the breeding box side net to block meshes or shellfish scratches fish skin is avoided; and the culture box can be supported.

The invention discloses an active peptide remover for improper makeup on the surface of a stone cultural relic, a method and application, and relates to the technical field of stone cultural relic protection. The active peptide remover comprises 0.5-5% of surface active peptide, 0.1-1% of auxiliary components and the balance of deionized water, the surface active peptide is one or more of soybean peptide, deep sea fish skin peptide, sea cucumber peptide, wheat peptide, corn peptide, silk fibroin peptide and whey protein peptide; the active peptide remover comprises an auxiliary component and an auxiliary component, the auxiliary component is a stabilizer and / or a pH regulator, the stabilizer is one or more of xanthan gum, carrageenan, bentonite and kaolin, the pH regulator is citric acid or sodium hydroxide, and the pH value of the active peptide remover is 6.5-7.5. According to the active peptide remover, the stone cultural relics can be prevented from being damaged by high temperature of steam and a sand blasting medium; and the binding force of makeup color and stone can be destroyed, and the pressure, temperature and time required during steam cleaning and sand blasting removal can be reduced.

The utility model relates to the technical field of kitchen utensils, in particular to a fish skin black skin removing device which comprises an inner cylinder, an outer cylinder, a handle, a head fixator and a tail fixator. The inner cylinder is elastically connected with the outer cylinder, and the head fixator and the tail fixator are rotationally connected with the outer cylinder; the head fixator is used for clamping the head of a fish, the tail fixator is used for clamping the tail of the fish, the handle is arranged on the side where the head fixator is located, and the handle is fixedly connected with the head fixator; a skin scraper is arranged on the inner surface of the inner cylinder, and the inner cylinder is formed by rolling up an elastic plate. Fish is placed in the inner cylinder, the head fixing device and the tail fixing device are used for fixing the fish body, the handle is rotated, mucus and black skin on the fish body are scraped off by the skin scraping device, the mucus and black skin treatment efficiency of the device is higher, the treatment effect is better, and the effect is particularly remarkable under the condition of large-scale treatment of the leiocassis longirostris.

1. The name of the design product: fish skin packaging bag. 2. The use of the design product: containing fish skin. 3. The design points of the design product: the combination of patterns and colors. 4. The picture or photo that best shows the design points: front view. 5. The design claimed contains colors.

1. Name of the designed product: packing bag (fish skin). 2. Use of the designed product: for packing products. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo showing the design points: perspective view 1.

The application discloses collagen tripeptide and a preparation method and application thereof, and belongs to the technical field of bioengineering. The preparation method comprises the following steps: obtaining bacillus velezensis with high collagenase yield through separation and identification, the bacillus velezensis belongs to probiotics, and high-activity collagenase is obtained through fermentation culture and purification; taking shark cartilage or blue fish skin as raw materials, and obtaining collagen tripeptide through the following steps of combined enzymolysis of alkaline protease and collagenase after pretreatment, inactivation, centrifugal separation and freeze-drying. The collagen tripeptide prepared by the application has a molecular weight of less than 500 Dalton, contains active components such as GHK tripeptide, is easy to absorb and has high safety, and can be used for preparing antioxidant products, anti-inflammatory products, memory-enhancing products or immunity-enhancing products. The application solves the problems of unsafe collagenase source and low collagen tripeptide preparation efficiency, and has a good industrial application prospect.

The present application relates to a kind of preparation method of collagen tripeptide liquid and its application, belong to the technical field of bioactive peptide, preparation method includes the following steps: A1, fish skin raw material is pretreated, and fish skin homogenate is obtained;A2, fish skin homogenate pH value is adjusted to neutral, and 1-4% of the complex protease of fish skin quality is added to carry out enzymolysis, and collagen peptide crude extract is obtained;A3, enzyme is killed, and centrifugation is carried out, and supernatant is collected to carry out nanofiltration, and obtain filter component 1 and cut-off component 1;A4, cut-off component 1 is placed in hydrochloric acid solution, and hydrolysis is obtained hydrolysate;Hydrolysate is adjusted to neutral, then nanofiltration is carried out, and obtain filter component 2 and cut-off component 2;A5, after filter component 1 and filter component 2 are mixed, nanofiltration is carried out, and cut-off component 3 is collected, and collagen tripeptide liquid is obtained.The collagen tripeptide liquid prepared in the present application has high collagen tripeptide yield, and the purity is also effectively improved.

1. The name of the design product: packaging bag (non-Hyman - fish skin with pickled peppers). 2. The use of the design product: used as a packaging bag. 3. The design points of the design product: in the combination of shape, pattern and color. 4. The picture or photo that best indicates the design points: front view. 5. The design for which protection is sought includes color. 6. The design product is a flat product, and other views are omitted.

The invention relates to a salmon fish skin xanthine oxidase inhibitory peptide and application thereof. The preparation method comprises the following steps: by taking salmon fish skin as a raw material, performing enzymolysis on the degreased salmon fish skin subjected to degreasing treatment by using pepsin to obtain a salmon fish skin xanthine oxidase inhibitory peptide compound, and separating and purifying the salmon fish skin xanthine oxidase inhibitory peptide compound to obtain the peptide DLGP. The salmon skin xanthine oxidase inhibitory peptide DLGP prepared by the invention has xanthine oxidase inhibition and uric acid reduction activity, has no toxic effect on HK-2 cells, has a uric acid reduction effect on hyperuricemia HK-2 cells, and can be used as a functional component to be applied to products for reducing uric acid or resisting hyperuricemia.

The invention belongs to the technical field of food processing, and relates to a preparation method and application of low-fishy-smell aroma-enhanced fish skin collagen peptide. The invention provides a preparation method of low-fishy-smell aroma-enhanced fish skin collagen peptide, which comprises the following steps: S1, flushing fish skin, mincing, and adding water; s2, adding neutral protease and flavourzyme to carry out composite enzymolysis; s3, after the enzymolysis is finished, respectively adding glucose, saccharomyces cerevisiae and lactobacillus plantarum, carrying out short-time mixed fermentation to produce fragrance, and filtering to remove impurities to obtain filtrate; s4, performing impurity removal and decoloration on the filtrate through a nanofiltration membrane; s5, adding 95% ethanol into the filtrate treated by the nanofiltration membrane, then carrying out low-temperature deposition, and collecting the precipitate; and S6, adding distilled water into the precipitate for dissolving, and freeze-drying after the residual alcohol is completely volatilized, thereby obtaining the product. The fish skin collagen peptide with fermentation fragrance, less fishy smell, high solubility, low molecular weight, light color and luster and high freshness can be prepared.

The invention relates to the technical field of ship antifouling materials and environmental protection, and discloses an environment-friendly and efficient surface fish-skin-imitated protective material, a construction method and a product. The environment-friendly and efficient surface fish-skin-imitated protective material is prefabricated in a factory and comprises a release layer and a coiled material of an elastic low-surface-energy fish-skin-imitated coating protective film layer; the elastic low-surface-energy fish-skin-imitating coating protective film layer is formed by coating a release layer with a low-surface-energy fish-skin-imitating coating in advance and drying the coating, and then winding the coating and the release layer together to form a coiled material which is continuous in the length direction. By means of material structure simplification and collaborative improvement of a formula and a construction process, the effects of saving labor, labor and materials, being high in efficiency, environmentally friendly, long in service life and the like are achieved, the cost of multiple links such as material manufacturing, transportation and construction is greatly reduced, and the requirements of industries such as manufacturing and maintenance of ships and other marine traffic tools, equipment and facilities can be met.

The invention discloses a takifugu fish skin collagen peptide and an extraction method thereof, and the extraction method comprises the following steps: adding takifugu fish skin without thorns into a calcareous alkali solution for stirring and washing to remove impure proteins and pigments, filtering and washing with pure water to obtain takifugu fish skin subjected to alkali treatment; adding an acidic extracting solution into the alkali-treated takifugu fish skin, and carrying out hydrothermal reaction under the protection of inert gas, so that the alkali-treated takifugu fish skin is fully dissolved to obtain a takifugu fish skin polypeptide extracting solution; the takifugu fish skin polypeptide extracting solution is filtered, dialyzed and subjected to vacuum freeze drying, and the fish skin collagen peptide is obtained. Under the condition of low energy consumption, the defects that an existing enzymolysis method is high in cost and complex in process and a traditional chemical method seriously damages a polypeptide structure are overcome, and the three-helix structure of the extracted collagen peptide can be effectively protected, so that the collagen peptide can be applied to the fields of cosmetics, functional food and medical materials.