Extraction and preparation method for micromolecule fishskin collagen peptide

A technology of fish skin collagen and collagen peptide, which is applied in the preparation method of peptide, chemical instrument and method, peptide and other directions, can solve the problems of wide molecular weight distribution, high price of protease, limited application scope, etc. The effect of reducing the amount of protease and shortening the time of enzymatic hydrolysis

Active Publication Date: 2012-10-03
NINGDE XIAWEI FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the high price of protease, if the extraction process is not properly designed, not only a large amount of protease needs to be added, but also the enzymatic hydrolysis time is long, resulting in high produc

Method used

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  • Extraction and preparation method for micromolecule fishskin collagen peptide
  • Extraction and preparation method for micromolecule fishskin collagen peptide
  • Extraction and preparation method for micromolecule fishskin collagen peptide

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Add 50L of 0.01M NaOH solution containing 20% ​​(v / v) absolute ethanol to 10 kg of tilapia fish skin, soak for 12h at 20°C, rinse with tap water, then add 50L of 0.05M HCl solution Soak at 20°C for 1h, rinse with tap water until the pH is 3, use a separate high-consistency refiner to grind the treated fish skin, heat at 90°C for 60min, and then use sodium hydroxide to adjust its pH Adjust to 8.0, then add 2 g of collagenase, enzymolysis at 55°C for 4h, use hydrochloric acid to adjust the pH of the enzymolysis solution to 5.0, add 20 g of papain, after enzymolysis at 55°C for 1h, The ultrafiltration membrane with a molecular weight cut-off of 5000 Daltons is used for separation, and the solution that fails to pass through the ultrafiltration membrane is recovered to the papain enzymatic hydrolysis step for continued use. Desalting and concentrating a sodium filter membrane with a molecular weight cut-off of 200 Daltons can obtain 7L of collagen peptide solution with a Br...

Embodiment 2

[0033] Add 40L of 0.05M NaOH solution containing 20% ​​(v / v) absolute ethanol to 10 kg silver carp skin, soak at 4°C for 24h, rinse with tap water, add 70L of 0.20M HCl solution in the Soak at 4°C for 3 hours, rinse with tap water until the pH is 5, use a separate high-consistency refiner to grind the treated fish skin, heat at 100°C for 30 minutes, and then use sodium hydroxide to adjust its pH to 9.0, then add 5 g of collagenase and 5 g of alkaline protease, enzymolysis 1h at 45 ° C, utilize hydrochloric acid to adjust the pH of the enzymolysis solution to 6.0, add 50 g of bromelain, and hydrolyze at 50 ° C After 4 hours of enzymatic hydrolysis, use an ultrafiltration membrane with a molecular weight cut-off of 2000 Daltons to separate, the solution that fails to pass through the ultrafiltration membrane is recovered to the bromelain enzymatic hydrolysis step for continued use, and the filtrate that passes through the ultrafiltration membrane is heated at 90°C Inactivate the...

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Abstract

The invention discloses an extraction and preparation method for micromolecule fishskin collagen peptide, which comprises the following technical steps of: taking the fishskin of freshwater fish as raw material; removing non-collagenous protein; carrying out steps of degreasing, deodorizing, smashing and the like; under the acidic condition, carrying out heating hydrolysis processing to collagenous protein; controlling the degradation degree of the collagenous protein with an enzymolysis method; obtaining a target micromolecule collagen peptide with a membrane separation method; recycling protease, and then carrying out nanofiltration desalting; and after activated carbon binding resin is decolored and deodorized, carrying out spray drying to obtain the colorless and tasteless micromolecule fishskin collagen peptide. The extraction and preparation method disclosed by the invention has a simple technology, the protease is recycled by membrane separation, and therefore the cost is saved. The prepared micromolecule collagen peptide is colorless and tasteless and can be widely applied to industries relevant to the health of the human body, such as health care food, biomedicine, and cosmetics.

Description

technical field [0001] The present invention relates to the field of preparation of small-molecule collagen peptides extracted from freshwater fish skin, in particular to an extraction of colorless and odorless small-molecule fish skin collagen peptides prepared by controlled enzymatic hydrolysis, membrane separation, and activated carbon combined with resin decolorization Preparation. Background technique [0002] Generally, small molecule collagen peptides can be widely used as active ingredients in industries related to human health, such as medicine, high-end cosmetics, and health food. At present, the raw materials used to prepare small molecule collagen peptides at home and abroad mainly come from pigskin and cowhide. However, due to the panic of mad cow disease, pig foot-and-mouth disease and religious beliefs, the preparation of small molecule collagen peptides from aquatic product processing waste, especially fish skin, has attracted continuous attention. Accordin...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K2/00C07K1/36C07K1/34
Inventor 翁武银王宝周曹敏杰刘光明苏文金
Owner NINGDE XIAWEI FOOD
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