34 results about "Silver carp" patented technology

The invention discloses a silver carp 20K liquid phase chip and application thereof, the liquid phase chip comprises a probe combination of 20,909 SNP molecular markers covering a whole genome, the physical positions of the 20,909 SNP molecular markers are determined based on sequence alignment of the silver carp genome (GCA041475455.1), and the site information of the 20,909 SNP molecular markers is shown in the specification table 1. The invention provides a silver carp first type whole genome SNP liquid phase breeding chip, which effectively reduces the application cost of silver carp in genetic diversity analysis, QTL positioning, GWAS analysis and the like in scientific research, solves the problem that no applicable product exists in silver carp molecular auxiliary breeding and whole genome selective breeding, accelerates the progress of silver carp basic research and breeding, and has a wide application prospect. The method is of great significance in improving the utilization efficiency of silver carp germplasm resources, accelerating the breeding process of excellent characters and improving the economic value and environmental adaptability of bred varieties.

According to the high-gel-quality minced fillet gel and the preparation method thereof, frozen minced silver carp is unfrozen and then subjected to ice-temperature vacuum dehydration and ultrahigh-pressure homogenization treatment, a myofibrillar protein molecular chain is promoted to stretch through mild shearing force, and a TG enzyme acting site is fully exposed; then chopping and mixing with table salt, starch, TG enzyme coated porous starch-curdlan / sodium alginate oligosaccharide nanosphere dispersion liquid and ice water at low temperature, shielding TG enzyme by nanospheres through a core-shell structure at low temperature so as to avoid advanced activation, and accurately releasing during heating so as to catalyze crosslinking; finally, step-by-step heating gelation is performed, so that the strength and uniformity of minced fillet gel are remarkably improved, the flavor is improved, and the method is suitable for industrial production.

The invention discloses a preparation method and application of anti-photoaging collagen polypeptide. The preparation method comprises the following steps: unfreezing and cleaning silver carp skin, airing and cutting; naHCO3 is added, and soaking is carried out; adding Na2CO3 and carrying out ultrasonic impurity removal; performing ultrasonic-assisted treatment; adding protease for enzymolysis treatment; establishing an enzymolysis kinetic model under the alkaline protease condition; standing, cooling and centrifuging the enzymatic hydrolysate, collecting supernate, filtering, and performing vacuum freeze drying to obtain faint yellow SCSCP; the light aging resistant peptide SCSCP02 is separated, and the active peptide sequences of the light aging resistant peptide SCSCP02 are GPPGPPGTPGPQ, SGLPGPIGPPGPR and GLPGPIGPPGPR. The active substances which are extracted from the fish skin and have the anti-photoaging effect can reduce the risk of skin light damage caused by UVR by acting on various treatment targets, resource waste can be reduced, environmental pollution caused by biological resources can be relieved, and sustainable development of the industry can be promoted.

The invention discloses an application of plasma activated water in a minced fillet product. The invention provides a preparation method of plasma activated water. The plasma activated water is prepared by a plasma generating device under certain parameters. The plasma activated water is added into the unrinsed surimi to replace part of added water in the traditional surimi product, so that the salt-reduced surimi product is prepared. The addition mode maintains the gel strength of the salt-reduced surimi product and effectively improves the color and luster of the product on the premise of maintaining the whole moisture content of the surimi product.

The invention relates to the technical field of hypoxia-resistant silver carps, in particular to a molecular marker associated with hypoxia resistance of silver carps, a gene and application. The molecular marker comprises C and T single nucleotide polymorphism of 29647765th nucleotide of the LG8 chromosome number of silver carp, and the gene is CTSC. The molecular marker and the gene are both associated with hypoxia resistance. The hypoxia-resistant silver carp variety can be obtained by screening CC genotype individuals at the molecular marker or screening individuals with low gene expression quantity and expression deficiency, and technical support is provided for breeding hypoxia-resistant silver carp, increasing the breeding yield and reducing the economic loss of breeding.

The invention relates to the technical field of fry domestication, in particular to domestication bait and domestication method for mandarin fish fries. According to the specific technical scheme, the domestication bait for the mandarin fish fries comprises live crucian carp fries and silver carp fries, half-dead crucian carp fries and half-dead silver carp fries, mandarin fish powder, a mixture of the dead crucian carp fries and the dead silver carp fries and mandarin fish feed 0 #; a phagostimulant is added into the powder, and the phagostimulant is one of squid paste, betaine and hermetia illucens pulp. According to the three phagostimulants disclosed by the invention, the feeding domestication efficiency can be improved, the squid paste has the most obvious effect in the aspect of promoting the early growth performance and is excellent in related physiological metabolism indexes, and the betaine relieves the liver metabolism burden in the mandarin fish domestication process.

The invention relates to the technical field of food processing, in particular to marinating soup for sauce-marinated chub, the sauce-marinated chub prepared from the marinating soup and a preparation method of the sauce-marinated chub. According to the preparation method of the novel marinating soup for the sauce-stewed chub, the fishy smell of the chub can be better removed by the marinating soup prepared by the method, and the quality of the sauce-stewed chub can be improved. Based on the marinated soup prepared by the method, the invention provides the preparation method of the sauce-marinated chub, and the sauce-marinated chub prepared by the method has the advantages of obviously reduced fishy smell, better delicate flavor, rich marinated taste, better overall smell, moderate hardness, chewiness, good chewiness, compact fish block structure and better appearance and color. Moreover, the method can reduce nutrient loss of fish meat and formation of potential harmful substances, the content of volatile basic nitrogen is obviously lower than that of a conventional marinating method, and the eating quality of the sauce-marinated chub can be effectively improved.

The utility model discloses a spotted silver carp powder feeding device which comprises a breeding net, a supporting frame is connected to an opening in the top of the breeding net, a floating ring is arranged in the middle of the opening of the breeding net, a feeding opening is formed in the middle of the floating ring, and a net bag used for storing powder is connected to the lower portion of the floating ring. An inner supporting structure used for forcing powder to support the net bag from inside to outside is arranged in the net bag. According to the spotted silver carp powder feeding device, spotted silver carps can be distributed on the outer side of the net bag for feeding, concentrated food grabbing of the spotted silver carps is avoided, and the breeding effect is good.

The invention relates to the field of aquatic food traceability, in particular to a system for identifying wild and culture sources of chubs and application of the system. The system comprises: a sample processing module, wherein the sample processing module is at least used for processing a chub sample to obtain original data of a polypeptide as shown in any one of SEQ ID NO.1-4, and obtaining proteome component response of a to-be-detected sample; the data processing module is at least used for importing the proteome component response of the sample to be detected into an OPLS-DA classification discriminant analysis model to obtain a score plot for discriminant analysis of subsequent sample data; and the result output module is at least used for outputting a result. The system can be effectively used for identifying wild and culture sources of silver carps, is high in stability and specificity, does not need to use a large instrument, is low in detection cost, and has a wide application prospect in the aspect of aquatic product traceability.

This invention discloses a method for co-culturing giant freshwater prawns (Macrobrachium rosenbergii) with shrimp, grass, and fish based on probiotic regulation, and its application. This invention constructs a three-dimensional ecological system with shrimp as the main body, grass as the protector, and fish as a supplement. Elodea and water spinach purify the water, silver carp and crucian carp decompose uneaten feed and feces, and a compound microbial preparation of Lactococcus gasseri C6a2 regulates water quality, while a biological bottom conditioner improves the bottom sediment, achieving precise ecological regulation of the aquaculture environment. This invention does not rely on chemical agents, effectively inhibits ammonia nitrogen accumulation, reduces disease incidence, and significantly improves the survival rate and size of giant freshwater prawns, achieving a win-win situation for both ecological and economic benefits.

The invention discloses an edible fish collagen-based antibacterial active membrane as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing silver carp skin collagen; the preparation method comprises the following steps: preparing an epsilon-polylysine / sulfobutyl-beta-cyclodextrin / carvacrol inclusion compound; the preparation method comprises the following steps: dissolving an epsilon-PL / SBE-beta-CD / CAR inclusion compound and silver carp skin collagen in ultrapure water, carrying out a stirring reaction at a certain temperature, adding glycerin, and carrying out stirring so as to obtain a membrane casting solution; and performing ultrasonic degassing on the membrane casting solution, injecting the membrane casting solution into a mold, storing the mold in a constant-temperature and constant-humidity box with the temperature of 26 DEG C and the relative humidity of 50% for 48 hours, and uncovering the membrane to obtain the edible fish collagen-based antibacterial active membrane. The preparation method is simple in technological process, and the prepared edible fish collagen-based antibacterial active film is high in antibacterial performance and good in mechanical performance. The invention provides an effective prevention and treatment strategy for the problem of microbial pollution.

This invention relates to the field of aquatic compound feed technology, specifically to a special feed for mullet, silver carp, and golden carp cultured in saline-alkali land in Northwest China. The feed is characterized by being composed of the following raw materials by weight percentage: 10%–14% alfalfa meal, 16%–22% soybean meal, 16%–22% corn meal, 5%–7% molasses, 1%–3% yeast powder, 2%–5% lactic acid bacteria preparation, 2%–5% premixed feed for aquatic life in saline-alkali land in Northwest China, 1%–4% fish oil, and 1%–5% vegetable oil. This invention solves the problems of low survival rate, high feed conversion ratio, easy antagonistic inactivation of probiotics, fish oil oxidation, poor water resistance of pellets, and insufficient regional adaptability of traditional feeds. Compared with commercially available ordinary marine fish feeds, the survival rate of juvenile fish is increased by 15% to 30%, the feed conversion ratio is reduced by 10% to 20%, the Vibrio inhibition rate is ≥80%, the power consumption per ton of feed is reduced by 25% to 35%, and the raw material cost is reduced by 30% to 45%. It is suitable for large-scale aquaculture in saline-alkali land in Northwest China and has important value for improving the quality and efficiency of fisheries, ecological restoration, and rural revitalization.

The invention discloses a snail-shrimp-grass green three-dimensional planting and breeding and blue-green algae prevention and control method. The planting and breeding system is constructed through layered utilization of water resources. Chubs are stocked on the upper layer, crayfishes are bred on the middle-lower layer, bellamya is put on the bottom layer, and an underwater three-dimensional space is constructed in cooperation with waterweed and high-temperature-resistant vallisneria nana. According to the method, specific fry specifications, bait formulas and feeding management are adopted, the three-dimensional oxygenation technology is combined, the yield per mu of snails is 800-1000 jin, the yield per mu of crayfishes is 200 jin, more than 60 jin of egg-carrying crayfishes are obtained in batches per mu, and compared with a traditional mode, the benefit is increased by 150%. Meanwhile, by means of the synergistic effect of the vallisneria nana, the bellamya and the chub, growth of the blue-green algae is inhibited, the blue-green algae outbreak risk is reduced by 60-70%, and peripheral ponds can be connected in series to purify tail water for cyclic utilization. The mode gives consideration to economic benefits and ecological benefits, reduces the breeding cost, and realizes peak-shifting high-price marketing of the crayfishes.

The invention relates to a Chryseobacterium-derived protein glutaminase PG3, a coding gene and an application thereof, and belongs to the technical field of biological genetic engineering. The amino acid sequence of the protein glutaminase PG3 is as shown in SEQ ID No. 1, and the cDNA sequence of the coding gene of the protein glutaminase PG3 is as shown in SEQ ID No. 2. A large amount of protein glutaminase PG3 from Chryseobacterium is prepared through an Escherichia coli expression system, and the protein glutaminase is used for carrying out enzymatic deamidation modification on the spotted silver carp myofibrillar protein PG3, so that the solubility of the spotted silver carp myofibrillar protein under a salt-free system condition is remarkably improved. The invention provides an important tool enzyme for protein deamidation modification, and lays a theoretical foundation for development of low-salt fish protein processed products.

The invention provides application of protein glutaminase to preparation of salt-reduced, fresh-increased and fishy-smell-removed fish protein gel products, and belongs to the field of food processing. A large amount of protein glutaminase PG3 derived from Chryseobacterium is prepared through a heterologous expression technology, the protein glutaminase PG3 is used for carrying out enzymatic deamidation modification on spotted silver carp MP, and finally, a spotted silver carp MP solution obtained after deamidation is heated and refrigerated to prepare the salt-free thermally-induced MP gel. In addition, the contents of substances with fishy smell, such as hexanal, nonanal and 1-octylene-3-alcohol, in the salt-free heat-induced MP gel are remarkably reduced, and the delicate flavor perception is improved. The method not only plays an important role in fishy smell removal and freshness enhancement of the fish protein gel, but also provides scientific basis and technical support for healthy and sustainable development of high-quality salt-reduced MP gel products.

The present application belongs to the technical field of polypeptide processing, and particularly relates to a preparation method of protein peptide capable of improving flavor and function; the method uses silver carp as raw material, uses food-grade protease, and obtains full fish protein peptide through moderate enzymolysis; under mild conditions, nitrogen is used to replace air to perform glycosylation reaction, which can effectively improve the flavor and antioxidant function of the full fish protein peptide; compared with the original full fish protein peptide and the full fish protein glycopeptide prepared by using air for glycosylation reaction, the full fish protein glycopeptide prepared by the method has lighter fishy smell and bitterness, can effectively improve the acceptance of the public, has strong antioxidant function and good storage stability, and can be widely applied in special food and nutritional food.

The invention discloses a co-cultivation method of large-scaled paraloach and mandarin fish seedlings, and belongs to the field of freshwater fish seedling cultivation. The operation steps are as follows: 1. preparing a cultivation pond, 2. inducing spawning and hatching of mandarin fish, 3. matching breeding of large-scaled paraloach, and 4. co-cultivation of large-scaled paraloach seedlings and mandarin fish seedlings. The invention utilizes the characteristics of large-scaled paraloach that the breeding cycle is long, high-density cultivation is possible, and it is rich in nutrition to co-cultivate large-scaled paraloach seedlings and mandarin fish seedlings; the unique horizontal swimming period and morphological characteristics of large-scaled paraloach seedlings are easy to be preyed on and digested by mandarin fish seedlings, which can improve the survival rate of mandarin fish seedlings during the opening period; large-scaled paraloach seedlings are rich in nutrition and high in protein content, which promotes the rapid growth of mandarin fish seedlings, and are high-quality bait fish for mandarin fish fry to open. The invention supplies a sufficient amount of palatable bait fish when the mandarin fish opens its mouth, avoids daily catching of silver carp, bream and other fry to feed the mandarin fish fry, saves the heavy workload of manually catching bait fish fry, creatively prolongs the breeding cycle of the mandarin fish fry, and greatly increases the annual output of the mandarin fish fry.

The present application relates to the technical field of silver carp identification or confirmation, and specifically to amino acid biomarkers and applications of wild and farmed silver carp. The biomarkers include at least one of proline, valine, leucine, Nε-formyl-L-lysine, γ-aminobutyric acid, N-formylglycine, N-acetyl-L-lysine, creatinine, DL-2-aminocaprylic acid, and 5-aminolevulinic acid. Biomarkers can be accurately used to distinguish wild and farmed silver carp, provide scientific basis and technical support for relevant law enforcement departments, strengthen the supervision and crackdown on illegal fishing in the Yangtze River Basin, reduce overfishing of wild resources, and provide a technical basis for better management and protection of silver carp resources.

The present invention discloses a silver carp skin and bone ACE inhibitory peptide, a preparation method and application thereof, and relates to the technical field of ACE inhibitory peptides. The preparation method of the silver carp skin and bone ACE inhibitory peptide provided by the present invention is as follows: steam explosion treatment is performed on a mixture of silver carp fish skin and fish bones, and the intermediate layer is centrifuged to obtain a steam explosion liquid; the pH of the steam explosion liquid is adjusted, and alkaline protease is added to the mass of the steam explosion liquid for enzymatic hydrolysis until the pH is neutral; then, flavor protease is added to the mass of the steam explosion liquid for enzymatic hydrolysis to obtain a primary enzymatic hydrolysis product containing the silver carp skin and bone ACE inhibitory peptide. Five ACE inhibitory peptides are obtained by ultrafiltration and screening analysis of the primary enzymatic hydrolysis products. These primary enzymatic hydrolysis products, ultrafiltration products and ACE inhibitory peptides all have good ACE activity inhibition rates and can be widely used in the preparation of blood pressure lowering drugs.

The utility model discloses a silver carp and bighead carp hook with a rotating anti-entanglement tube capable of fixing the direction of a hook tip, comprising a fishing plate and a sub-line anti-entanglement tube. A plurality of plugging posts are evenly distributed in an annular manner on the surface of the fishing plate. The sub-line anti-entanglement tube is provided with a plurality of groups and respectively plugged into the surface of the plugging posts. A plurality of sub-line holes are evenly distributed in an annular manner on the surface of the fishing plate. A main line hole is provided in the middle of the fishing plate and a main line is movably passed through the main line hole. One end of the main line is bound to a fishing rod line group and the other end is bound to a plurality of sub-lines. After the sub-lines are bound to the sub-line holes, they pass through the sub-line anti-entanglement tube and are connected to the fish hook. A rubber plug is inserted into the sub-line anti-entanglement tube. The sub-line and the fish hook can be driven to rotate by rotating one end of the sub-line anti-entanglement tube plugged with the plugging post, thereby adjusting the direction of the fish hook so that the hook tip faces outwards. After adjustment, the sub-line can be fixed by the inserted rubber plug, thereby orienting the direction of the hook tip. By making the hook tip face outwards, the fish hook is easier to penetrate into the fish mouth, thereby reducing the possibility of fish detachment and facilitating replacement of the worn sub-line.

The invention discloses a preparation method and application of a silver carp skin collagen-based antibacterial active coating. The silver carp skin collagen-based antibacterial and antioxidant active coating is prepared from the following raw materials: collagen, epsilon-polylysine, carvacrol, sulfobutyl-beta-cyclodextrin and ultrapure water; wherein the stoichiometric ratio of the sulfobutyl-beta-cyclodextrin to the epsilon-polylysine is 40: 1, and the mass ratio of the sulfobutyl-beta-cyclodextrin to the carvacrol is 2: 1. The silver carp skin collagen is used as a coating substrate, and the natural antibacterial peptide epsilon-polylysine and the plant essential oil carvacrol play a synergistic antibacterial role. In addition, the epsilon-polylysine and the sulfobutyl-beta-cyclodextrin are crosslinked through electrostatic interaction. The silver carp skin collagen-based antibacterial antioxidant active coating prepared by the invention does not contain a reagent toxic to a human body, is high in safety, and has a good coating preservation effect on fresh fish balls. In addition, the method is simple in technological process and can be easily applied to the field of aquatic food preservation.

The invention discloses a making method of a Wujiang tofu fish pot bottom, and belongs to the field of food processing. The preparation method comprises the following steps: preparing silver carp, Wujiang bean curd, self-made glutinous rice cake chili (slightly spicy 500g / pot, medium spicy 750g / pot and extra-spicy 800g / pot) with graded dosage, 1000g of vegetable oil, 500g of lard oil and the like according to a ratio, pickling fish blocks, soaking and pretreating bean curd, frying materials at controlled temperature, decocting the fish first and then the bean curd, embellishing and taking out of the pot. The problems of traditional pungency degree fluctuation, single flavor and difficulty in copying are solved, the pungency degree is controllable, the flavor is stereoscopic, and the method adapts to family and catering scenes.

The invention discloses a method for promoting lake pollution reduction, carbon sequestration and sink increase through combination of bottom groove construction and silver carp, which comprises the following steps: constructing a bottom groove downwards at the bottom of a lake in a range of 50-100m from lakeside wetland water to an outer edge water area of a middle and large lake; one or more closed silver carp captive rearing areas are formed at the position 120 m away from the lake shoreline to the lakeside wetland water towards the outer edge water area, and then silver carps are stocked in the silver carp captive rearing areas. Wetland plants grow to absorb water nutritive salt, atmosphere and water carbon dioxide and bicarbonate, and a large amount of carbon can be fixed in the plants; lake algae gather in a lakeside water area under the action of wind power, so that the algae filtering and feeding efficiency of silver carps captive on the lakeside is improved, and nitrogen, phosphorus, carbon and the like are fixed in fish bodies during fish growth; aquatic plant litter chippings and silver carp feces are brought into the constructed bottom groove by lake flow and stormy waves and are mutually buried with silt and other particles in the sedimentation process to form nitrogen phosphorus and carbon burying, and therefore the effects of reducing pollution, fixing carbon and increasing sink in lakes are achieved. The method can significantly improve the pollution-reducing, carbon-sequestration and sink-increasing effects of lakes.

This invention provides a targeted silver carp isg15 This invention relates to the sgRNA of a gene and its application in enhancing the resistance of fish to herpesviruses. It utilizes CRISPR / Cas9 technology to target and knock out the sgRNA of a silver carp. isg15-a Genes and isg15-b Genes were used to obtain F0 generation chimeras. The F0 generation underwent gynogenesis and selection to obtain... isg15 homozygous mutant silver carp isg15 ‑ / ‑ / ‑ Silver carp. After infection with crucian carp herpesvirus... isg15 ‑ / ‑ / ‑ The survival rate of silver carp was significantly increased; the expression of isg15 protein in tissues was significantly reduced; and viral genes were observed in the kidneys. 39ring The transcription level was significantly reduced. This demonstrates that the gene-edited fish prepared using the method of this invention have improved the crucian carp's resistance to herpesvirus, providing a foundation for disease-resistant breeding of silver crucian carp and also providing important reference value for disease-resistant breeding of other farmed fish.

The invention discloses a symbiotic freshwater shrimp green culture system and method based on localized film laying, and belongs to the technical field of aquaculture, and the technical scheme is as follows: geomembranes are laid on the pond wall and the pond bottom of a pond, the geomembranes are fixed through ground nails and gaskets, joints are bonded through hot melt adhesive, and the geomembranes form a main shrimp channel, a longitudinal auxiliary shrimp channel and a transverse auxiliary shrimp channel; the enclosed exposed pond bottom grids are used for planting aquatic plants; a long PVC drainage pipe with dense small holes is laid at the bottom of the pond, the front end is sealed by a plug, and the tail end is connected with a valve to control drainage; sectional type self-sinking nanometer aeration pipes are laid in the same direction of the drainage pipe, and multi-point sectional type bottom aeration is achieved through connection of a main ventilation pipe and a roots blower; the culture system comprises several nutritional levels of shrimps, aquatic plants, chubs and escargots to form a symbiotic system. The system is mainly used for green ecological breeding of freshwater shrimps, can effectively maintain the water body to be clear and guarantee stable growth of aquatic plants, realizes self-purification of water quality through multi-nutrition-level symbiosis, and improves the stability of the breeding system and the breeding capacity of a unit area.

The invention belongs to the technical field of polysaccharide preparation, and particularly relates to chub fishbone polysaccharide as well as a preparation method and application thereof. The method comprises the following steps: drying and crushing pretreated silver carp processing byproducts to obtain fish bone meal; then mixing and stirring with petroleum ether, degreasing and drying to obtain degreased silver carp bone meal; the preparation method comprises the following steps: dissolving degreased silver carp bone meal in water, adjusting the pH value to 4-10, adding papain, carrying out water bath heating extraction, centrifugation and rotary evaporation concentration, adding absolute ethyl alcohol, standing, centrifuging, redissolving, dialyzing and freeze-drying to obtain the silver carp bone polysaccharide. The preparation process is simple, the silver carp fishbone polysaccharide is high in purity and extraction rate, and the prepared silver carp fishbone polysaccharide has good free radical scavenging capacity and can be suitable for extraction of fishbone polysaccharide in processing by-products of grass carp and other freshwater fishes, and the utilization rate of the processing by-products of freshwater fish products is increased.

The invention discloses photodynamic antibacterial liquid for fresh keeping of silver carp balls as well as a preparation method and application of the photodynamic antibacterial liquid, and belongs to the technical field of food fresh keeping. According to the invention, two coating solutions are prepared to research the influence of the coating solutions on the quality change of the fish balls in the storage period: one is a chitosan coating solution loaded with cinnamyl aldehyde-tannic acid nano-emulsion, cinnamyl aldehyde and tannic acid are natural compounds and are proved to have strong oxidation resistance, chitosan has broad-spectrum antibacterial property, and the coating solution is a chitosan coating solution loaded with cinnamyl aldehyde-tannic acid nano-emulsion; the shelf life of the food can be obviously prolonged; according to the photodynamic mediated gallic acid antibacterial liquid, research shows that a polyphenol aqueous solution can be subjected to photooxidation after being subjected to light irradiation treatment, active oxygen is generated, and the antibacterial liquid has high antibacterial activity. The silver carp balls which are more easily deteriorated are researched and selected, and the fish balls are treated by adding a coating preservation technology, so that the influence of different coating solutions on the quality of the fish balls in the storage period and an antibacterial mechanism are researched, and an instructive effect is provided for later application of the coating technology and preservation of the fish balls.

The invention relates to the technical field of microbial fermentation, in particular to pediococcus acidilactici, a microbial preparation and application of the pediococcus acidilactici and the microbial preparation. According to the invention, a lactic acid bacteria strain, namely pediococcus acidilactici H24-8, with good acid production capacity is screened from naturally fermented minced silver carp fillet, and the preservation number of the pediococcus acidilactici H24-8 is CGMCC No.35481. The invention also discloses a preparation method of the pediococcus acidilactici H24-8. The gel property and flavor of the minced fillet treated by the pediococcus acidilactici H24-8 are remarkably improved, and an important technical support is provided for development of high-quality minced silver carp products. Experimental data show that the gel strength, the hardness, the chewiness and the elasticity of the minced silver carp treated by the pediococcus acidilactici H24-8 are all remarkably improved, the content of pleasant volatile flavor substances in the minced silver carp is remarkably increased, the content of fishy smell volatile flavor substances is remarkably reduced, and the obtained minced silver carp product has a wide market application prospect.

The invention relates to the technical field of silver carp judgment or confirmation, in particular to an amino acid biomarker for judging wild and cultured silver carps, a method and application. The biomarker comprises at least one of asparaginic acid, alanine, phenylalanine, histidine and arginine. The biomarker can accurately distinguish wild silver carps and cultured silver carps, provides scientific basis and technical support for related law enforcement departments, strengthens supervision and attack of illegal fishing behaviors in the Yangtze River basin, reduces excessive fishing of wild resources, and further provides a technical basis for better management and protection of silver carp resources.

1. The name of the product of this design: packaging bottle (Silver Carp Safe). 2. Purpose of this design product: used for containing veterinary drugs. 3. The design key point of this appearance design product lies in the combination of shape and pattern. 4. The picture or photo that best illustrates the key points of the design: main view.