19 results about "Silver carp" patented technology

According to the high-gel-quality minced fillet gel and the preparation method thereof, frozen minced silver carp is unfrozen and then subjected to ice-temperature vacuum dehydration and ultrahigh-pressure homogenization treatment, a myofibrillar protein molecular chain is promoted to stretch through mild shearing force, and a TG enzyme acting site is fully exposed; then chopping and mixing with table salt, starch, TG enzyme coated porous starch-curdlan / sodium alginate oligosaccharide nanosphere dispersion liquid and ice water at low temperature, shielding TG enzyme by nanospheres through a core-shell structure at low temperature so as to avoid advanced activation, and accurately releasing during heating so as to catalyze crosslinking; finally, step-by-step heating gelation is performed, so that the strength and uniformity of minced fillet gel are remarkably improved, the flavor is improved, and the method is suitable for industrial production.

The invention relates to the field of aquatic food traceability, in particular to a system for identifying wild and culture sources of chubs and application of the system. The system comprises: a sample processing module, wherein the sample processing module is at least used for processing a chub sample to obtain original data of a polypeptide as shown in any one of SEQ ID NO.1-4, and obtaining proteome component response of a to-be-detected sample; the data processing module is at least used for importing the proteome component response of the sample to be detected into an OPLS-DA classification discriminant analysis model to obtain a score plot for discriminant analysis of subsequent sample data; and the result output module is at least used for outputting a result. The system can be effectively used for identifying wild and culture sources of silver carps, is high in stability and specificity, does not need to use a large instrument, is low in detection cost, and has a wide application prospect in the aspect of aquatic product traceability.

This invention discloses a method for co-culturing giant freshwater prawns (Macrobrachium rosenbergii) with shrimp, grass, and fish based on probiotic regulation, and its application. This invention constructs a three-dimensional ecological system with shrimp as the main body, grass as the protector, and fish as a supplement. Elodea and water spinach purify the water, silver carp and crucian carp decompose uneaten feed and feces, and a compound microbial preparation of Lactococcus gasseri C6a2 regulates water quality, while a biological bottom conditioner improves the bottom sediment, achieving precise ecological regulation of the aquaculture environment. This invention does not rely on chemical agents, effectively inhibits ammonia nitrogen accumulation, reduces disease incidence, and significantly improves the survival rate and size of giant freshwater prawns, achieving a win-win situation for both ecological and economic benefits.

The invention discloses an edible fish collagen-based antibacterial active membrane as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing silver carp skin collagen; the preparation method comprises the following steps: preparing an epsilon-polylysine / sulfobutyl-beta-cyclodextrin / carvacrol inclusion compound; the preparation method comprises the following steps: dissolving an epsilon-PL / SBE-beta-CD / CAR inclusion compound and silver carp skin collagen in ultrapure water, carrying out a stirring reaction at a certain temperature, adding glycerin, and carrying out stirring so as to obtain a membrane casting solution; and performing ultrasonic degassing on the membrane casting solution, injecting the membrane casting solution into a mold, storing the mold in a constant-temperature and constant-humidity box with the temperature of 26 DEG C and the relative humidity of 50% for 48 hours, and uncovering the membrane to obtain the edible fish collagen-based antibacterial active membrane. The preparation method is simple in technological process, and the prepared edible fish collagen-based antibacterial active film is high in antibacterial performance and good in mechanical performance. The invention provides an effective prevention and treatment strategy for the problem of microbial pollution.

This invention relates to the field of aquatic compound feed technology, specifically to a special feed for mullet, silver carp, and golden carp cultured in saline-alkali land in Northwest China. The feed is characterized by being composed of the following raw materials by weight percentage: 10%–14% alfalfa meal, 16%–22% soybean meal, 16%–22% corn meal, 5%–7% molasses, 1%–3% yeast powder, 2%–5% lactic acid bacteria preparation, 2%–5% premixed feed for aquatic life in saline-alkali land in Northwest China, 1%–4% fish oil, and 1%–5% vegetable oil. This invention solves the problems of low survival rate, high feed conversion ratio, easy antagonistic inactivation of probiotics, fish oil oxidation, poor water resistance of pellets, and insufficient regional adaptability of traditional feeds. Compared with commercially available ordinary marine fish feeds, the survival rate of juvenile fish is increased by 15% to 30%, the feed conversion ratio is reduced by 10% to 20%, the Vibrio inhibition rate is ≥80%, the power consumption per ton of feed is reduced by 25% to 35%, and the raw material cost is reduced by 30% to 45%. It is suitable for large-scale aquaculture in saline-alkali land in Northwest China and has important value for improving the quality and efficiency of fisheries, ecological restoration, and rural revitalization.

The invention discloses a snail-shrimp-grass green three-dimensional planting and breeding and blue-green algae prevention and control method. The planting and breeding system is constructed through layered utilization of water resources. Chubs are stocked on the upper layer, crayfishes are bred on the middle-lower layer, bellamya is put on the bottom layer, and an underwater three-dimensional space is constructed in cooperation with waterweed and high-temperature-resistant vallisneria nana. According to the method, specific fry specifications, bait formulas and feeding management are adopted, the three-dimensional oxygenation technology is combined, the yield per mu of snails is 800-1000 jin, the yield per mu of crayfishes is 200 jin, more than 60 jin of egg-carrying crayfishes are obtained in batches per mu, and compared with a traditional mode, the benefit is increased by 150%. Meanwhile, by means of the synergistic effect of the vallisneria nana, the bellamya and the chub, growth of the blue-green algae is inhibited, the blue-green algae outbreak risk is reduced by 60-70%, and peripheral ponds can be connected in series to purify tail water for cyclic utilization. The mode gives consideration to economic benefits and ecological benefits, reduces the breeding cost, and realizes peak-shifting high-price marketing of the crayfishes.

The invention relates to a Chryseobacterium-derived protein glutaminase PG3, a coding gene and an application thereof, and belongs to the technical field of biological genetic engineering. The amino acid sequence of the protein glutaminase PG3 is as shown in SEQ ID No. 1, and the cDNA sequence of the coding gene of the protein glutaminase PG3 is as shown in SEQ ID No. 2. A large amount of protein glutaminase PG3 from Chryseobacterium is prepared through an Escherichia coli expression system, and the protein glutaminase is used for carrying out enzymatic deamidation modification on the spotted silver carp myofibrillar protein PG3, so that the solubility of the spotted silver carp myofibrillar protein under a salt-free system condition is remarkably improved. The invention provides an important tool enzyme for protein deamidation modification, and lays a theoretical foundation for development of low-salt fish protein processed products.

The invention provides application of protein glutaminase to preparation of salt-reduced, fresh-increased and fishy-smell-removed fish protein gel products, and belongs to the field of food processing. A large amount of protein glutaminase PG3 derived from Chryseobacterium is prepared through a heterologous expression technology, the protein glutaminase PG3 is used for carrying out enzymatic deamidation modification on spotted silver carp MP, and finally, a spotted silver carp MP solution obtained after deamidation is heated and refrigerated to prepare the salt-free thermally-induced MP gel. In addition, the contents of substances with fishy smell, such as hexanal, nonanal and 1-octylene-3-alcohol, in the salt-free heat-induced MP gel are remarkably reduced, and the delicate flavor perception is improved. The method not only plays an important role in fishy smell removal and freshness enhancement of the fish protein gel, but also provides scientific basis and technical support for healthy and sustainable development of high-quality salt-reduced MP gel products.

The present application belongs to the technical field of polypeptide processing, and particularly relates to a preparation method of protein peptide capable of improving flavor and function; the method uses silver carp as raw material, uses food-grade protease, and obtains full fish protein peptide through moderate enzymolysis; under mild conditions, nitrogen is used to replace air to perform glycosylation reaction, which can effectively improve the flavor and antioxidant function of the full fish protein peptide; compared with the original full fish protein peptide and the full fish protein glycopeptide prepared by using air for glycosylation reaction, the full fish protein glycopeptide prepared by the method has lighter fishy smell and bitterness, can effectively improve the acceptance of the public, has strong antioxidant function and good storage stability, and can be widely applied in special food and nutritional food.

The invention discloses a preparation method and application of a silver carp skin collagen-based antibacterial active coating. The silver carp skin collagen-based antibacterial and antioxidant active coating is prepared from the following raw materials: collagen, epsilon-polylysine, carvacrol, sulfobutyl-beta-cyclodextrin and ultrapure water; wherein the stoichiometric ratio of the sulfobutyl-beta-cyclodextrin to the epsilon-polylysine is 40: 1, and the mass ratio of the sulfobutyl-beta-cyclodextrin to the carvacrol is 2: 1. The silver carp skin collagen is used as a coating substrate, and the natural antibacterial peptide epsilon-polylysine and the plant essential oil carvacrol play a synergistic antibacterial role. In addition, the epsilon-polylysine and the sulfobutyl-beta-cyclodextrin are crosslinked through electrostatic interaction. The silver carp skin collagen-based antibacterial antioxidant active coating prepared by the invention does not contain a reagent toxic to a human body, is high in safety, and has a good coating preservation effect on fresh fish balls. In addition, the method is simple in technological process and can be easily applied to the field of aquatic food preservation.

The invention discloses a making method of a Wujiang tofu fish pot bottom, and belongs to the field of food processing. The preparation method comprises the following steps: preparing silver carp, Wujiang bean curd, self-made glutinous rice cake chili (slightly spicy 500g / pot, medium spicy 750g / pot and extra-spicy 800g / pot) with graded dosage, 1000g of vegetable oil, 500g of lard oil and the like according to a ratio, pickling fish blocks, soaking and pretreating bean curd, frying materials at controlled temperature, decocting the fish first and then the bean curd, embellishing and taking out of the pot. The problems of traditional pungency degree fluctuation, single flavor and difficulty in copying are solved, the pungency degree is controllable, the flavor is stereoscopic, and the method adapts to family and catering scenes.

This invention provides a targeted silver carp isg15 This invention relates to the sgRNA of a gene and its application in enhancing the resistance of fish to herpesviruses. It utilizes CRISPR / Cas9 technology to target and knock out the sgRNA of a silver carp. isg15-a Genes and isg15-b Genes were used to obtain F0 generation chimeras. The F0 generation underwent gynogenesis and selection to obtain... isg15 homozygous mutant silver carp isg15 ‑ / ‑ / ‑ Silver carp. After infection with crucian carp herpesvirus... isg15 ‑ / ‑ / ‑ The survival rate of silver carp was significantly increased; the expression of isg15 protein in tissues was significantly reduced; and viral genes were observed in the kidneys. 39ring The transcription level was significantly reduced. This demonstrates that the gene-edited fish prepared using the method of this invention have improved the crucian carp's resistance to herpesvirus, providing a foundation for disease-resistant breeding of silver crucian carp and also providing important reference value for disease-resistant breeding of other farmed fish.

The invention discloses a symbiotic freshwater shrimp green culture system and method based on localized film laying, and belongs to the technical field of aquaculture, and the technical scheme is as follows: geomembranes are laid on the pond wall and the pond bottom of a pond, the geomembranes are fixed through ground nails and gaskets, joints are bonded through hot melt adhesive, and the geomembranes form a main shrimp channel, a longitudinal auxiliary shrimp channel and a transverse auxiliary shrimp channel; the enclosed exposed pond bottom grids are used for planting aquatic plants; a long PVC drainage pipe with dense small holes is laid at the bottom of the pond, the front end is sealed by a plug, and the tail end is connected with a valve to control drainage; sectional type self-sinking nanometer aeration pipes are laid in the same direction of the drainage pipe, and multi-point sectional type bottom aeration is achieved through connection of a main ventilation pipe and a roots blower; the culture system comprises several nutritional levels of shrimps, aquatic plants, chubs and escargots to form a symbiotic system. The system is mainly used for green ecological breeding of freshwater shrimps, can effectively maintain the water body to be clear and guarantee stable growth of aquatic plants, realizes self-purification of water quality through multi-nutrition-level symbiosis, and improves the stability of the breeding system and the breeding capacity of a unit area.

The invention belongs to the technical field of polysaccharide preparation, and particularly relates to chub fishbone polysaccharide as well as a preparation method and application thereof. The method comprises the following steps: drying and crushing pretreated silver carp processing byproducts to obtain fish bone meal; then mixing and stirring with petroleum ether, degreasing and drying to obtain degreased silver carp bone meal; the preparation method comprises the following steps: dissolving degreased silver carp bone meal in water, adjusting the pH value to 4-10, adding papain, carrying out water bath heating extraction, centrifugation and rotary evaporation concentration, adding absolute ethyl alcohol, standing, centrifuging, redissolving, dialyzing and freeze-drying to obtain the silver carp bone polysaccharide. The preparation process is simple, the silver carp fishbone polysaccharide is high in purity and extraction rate, and the prepared silver carp fishbone polysaccharide has good free radical scavenging capacity and can be suitable for extraction of fishbone polysaccharide in processing by-products of grass carp and other freshwater fishes, and the utilization rate of the processing by-products of freshwater fish products is increased.

The invention discloses photodynamic antibacterial liquid for fresh keeping of silver carp balls as well as a preparation method and application of the photodynamic antibacterial liquid, and belongs to the technical field of food fresh keeping. According to the invention, two coating solutions are prepared to research the influence of the coating solutions on the quality change of the fish balls in the storage period: one is a chitosan coating solution loaded with cinnamyl aldehyde-tannic acid nano-emulsion, cinnamyl aldehyde and tannic acid are natural compounds and are proved to have strong oxidation resistance, chitosan has broad-spectrum antibacterial property, and the coating solution is a chitosan coating solution loaded with cinnamyl aldehyde-tannic acid nano-emulsion; the shelf life of the food can be obviously prolonged; according to the photodynamic mediated gallic acid antibacterial liquid, research shows that a polyphenol aqueous solution can be subjected to photooxidation after being subjected to light irradiation treatment, active oxygen is generated, and the antibacterial liquid has high antibacterial activity. The silver carp balls which are more easily deteriorated are researched and selected, and the fish balls are treated by adding a coating preservation technology, so that the influence of different coating solutions on the quality of the fish balls in the storage period and an antibacterial mechanism are researched, and an instructive effect is provided for later application of the coating technology and preservation of the fish balls.

The invention relates to the technical field of microbial fermentation, in particular to pediococcus acidilactici, a microbial preparation and application of the pediococcus acidilactici and the microbial preparation. According to the invention, a lactic acid bacteria strain, namely pediococcus acidilactici H24-8, with good acid production capacity is screened from naturally fermented minced silver carp fillet, and the preservation number of the pediococcus acidilactici H24-8 is CGMCC No.35481. The invention also discloses a preparation method of the pediococcus acidilactici H24-8. The gel property and flavor of the minced fillet treated by the pediococcus acidilactici H24-8 are remarkably improved, and an important technical support is provided for development of high-quality minced silver carp products. Experimental data show that the gel strength, the hardness, the chewiness and the elasticity of the minced silver carp treated by the pediococcus acidilactici H24-8 are all remarkably improved, the content of pleasant volatile flavor substances in the minced silver carp is remarkably increased, the content of fishy smell volatile flavor substances is remarkably reduced, and the obtained minced silver carp product has a wide market application prospect.

The application provides a high-gel-quality surimi gel and a preparation method thereof. After frozen white silver carp surimi is thawed, ice temperature vacuum dehydration and ultrahigh pressure homogenization treatment are carried out, the myofibrillar protein molecular chain is stretched by mild shearing force, and the TG enzyme action site is fully exposed; then, the surimi is chopped and mixed with salt, starch, TG enzyme porous starch-pectin oligosaccharide nanosphere dispersion and ice water at low temperature, wherein the nanosphere shields the TG enzyme at low temperature to avoid early activation, and is accurately released to catalyze cross-linking when heated; finally, the surimi is gelled by step heating, the strength and uniformity of the surimi gel are significantly improved, the flavor is improved, and the surimi gel is suitable for industrial production.

The invention relates to the technical field of fish genetic breeding, in particular to a method for improving carassius auratus gibelio herpesvirus resistance based on herc4 gene editing. The invention discloses three specific knockout targets of a serc4-B gene, and utilizes a CRISPR / Cas9 gene editing technology to specifically knock out the serc4-B gene of carassius auratus gibelio to obtain a mutant genotype individual with at least two alleles of serc4-B terminated in advance, namely the carassius auratus gibelio with high resistance to carassius auratus gibelio herpesvirus. According to the invention, the function of the herc4-B in the prussian carp is verified for the first time, a new germplasm with improved capability of resisting the herpesvirus of the prussian carp is rapidly obtained by knocking out the single gene of the herc4-B, and the herpesvirus of the prussian carp has important industrial value.

The invention discloses a free amino acid multi-index combination for identifying wild silver carps, a distinguishing method and application. The free amino acid multi-index combination comprises at least four of phosphoserine, taurine, threonine, sarcosine, beta-aminoisobutyric acid and hydroxyproline. The distinguishing method comprises the following steps: measuring the content of amino acids in a specific combination in a to-be-detected sample, inputting the content into a preset logistic regression model to calculate a wild probability value, and comparing the wild probability value with a distinguishing threshold value optimized and determined through an ROC curve so as to judge a source. According to the technical scheme, the specific biomarker for discriminating the source of the silver carp is defined for the first time, and the problem that a traditional experience discriminating method is high in subjectivity is solved. Through construction of the quantitative model, rapid, objective and high-precision discrimination is realized, and the discrimination accuracy of the optimal combination model can reach 97.8%. The method provides reliable technical support for identifying illegal fished catches in fishery administration law enforcement in the Yangtze River basin, and has important significance for protecting wild silver carp resources.