The invention discloses a preparation method of fishbone collagen
tripeptide, which comprises the following steps: soaking fishbone dust (30-60 meshes) in a
choline-
arginine eutectic
solvent, stirring, centrifuging or filtering, and collecting supernate; adding pure water with the volume 3-6 times that of the supernatant into the supernatant, standing for more than 24 hours, centrifuging or filtering again, and collecting the supernatant; carrying out tangential
filtration treatment by adopting a
nanofiltration membrane with the molecular weight cutoff of 200Da, recovering the eutectic
solvent in the filtrate through vacuum concentration, adding pure water into the trapped fluid, carrying out repeated
nanofiltration treatment to remove
solvent residues, and collecting the trapped fluid; and carrying out
spray drying on the trapped fluid to obtain a
powder product with the collagen
tripeptide content of more than 50%. Compared with a traditional
enzyme method for preparing fishbone collagen
tripeptide, the method disclosed by the invention has the advantages that the extraction rate is high, a deliming step is not needed, the
enzyme cost is saved, an extraction solvent is green and can be recycled, and the like.