28results about How to "Improve enrichment effect" patented technology

The invention belongs to the technical field of intestinal flora transplantation, and discloses an intestinal flora enrichment microsphere as well as a preparation method and application thereof and an enrichment method of intestinal flora. The intestinal flora enrichment microsphere provided by the invention specifically comprises a calcium alginate gel substrate and a porous adsorbent doped on the calcium alginate gel substrate, and the intestinal flora-enriched microspheres have a special pore size distribution situation, namely the pore size is gradually increased along the direction from the center to the outer layer. When the intestinal flora enrichment microspheres are applied to enrichment of intestinal flora, active flora cells can be effectively separated from small impurities such as intestinal contents and flora metabolites while the proportion of the high-activity flora cells is kept, and the intestinal flora enrichment microspheres have an excellent intestinal flora enrichment effect.

The invention relates to a shunting-in-situ matrix purification liquid-liquid electric extraction device and method and application, and relates to the technical field of chemical analysis. The extraction assembly comprises a push-and-pull device, an injector electrode and a shunt tube, the injector electrode comprises an injector electrode wire and an injector which are connected, the injector is used for containing conductive liquid, the push-and-pull device drives a piston of the injector to do push-and-pull motion, one end of the injector electrode wire is connected with the injector, and the other end of the injector electrode wire is grounded or connected with a power supply; one end of the shunt tube is communicated with the injector, and the other end is used for immersing a to-be-extracted object; the sample assembly comprises a sample container and a sample electrode wire, one end of the sample electrode wire is used for immersing an object to be extracted, and the other end is grounded or connected with a power supply; the sample container is used for accommodating a to-be-extracted substance; the equipment can reduce the risk of bubble generation through shunting, and improve the baseline stability and the electric extraction efficiency.

According to the ginseng extraction system and method provided by the invention, the dual goals of high purity and high yield of ginsenoside extraction are achieved through raw material adaptive parameter pre-optimization, self-adaptive cascade purification and activity protection, the enrichment effect of high-value monomer saponin is remarkable, and the loss of active ingredients is accurately controlled. According to the application, a detection-decision-regulation full-process closed-loop intelligent control system is constructed, near infrared spectrum rapid screening and HPLC-MS / MS accurate quantitative dual-mode online detection are fused, real-time and accurate monitoring of the saponin content is realized, and off-line detection and artificial experience parameters are not needed; the multi-target central control unit can automatically decide a purification path and process parameters according to detection data, and designs such as membrane pollution on-line monitoring, automatic backwashing and solvent circulating preheating are combined, so that continuous operation of the process is ensured, and production interruption caused by equipment failure and process fluctuation is avoided.

The invention belongs to the technical field of comprehensive utilization of valuable element mineral resources in low-grade uranium polymetallic ore, and particularly relates to a separation and recovery method for the valuable element mineral resources in the low-grade uranium polymetallic ore. The problem that low-grade uranium polymetallic ore resources are difficult to sort and utilize is solved. Monomer dissociation of minerals containing valuable elements is achieved through the ore grinding process, and enrichment of three mineral resources of rare earth, zirconium and niobium is successfully achieved by combining multiple mineral separation processes of reselection, magnetic separation and flotation. The process not only can effectively realize the resource comprehensive utilization of the low-grade rare earth associated ore, but also provides an important reference basis for the separation and recovery of valuable elements in the low-grade uranium polymetallic ore.

The invention discloses a preparation method of a high-purity vanadium electrolyte, particularly relates to a method for directly preparing the high-purity vanadium electrolyte from a high-acid vanadium-containing solution, and belongs to the technical field of hydrometallurgy. The method mainly comprises the steps of hydrogen peroxide-alkaline extractant synergistic extraction, catalyst catalysis hydrogen peroxide decomposition, sulfuric acid reextraction simple evaporation and concentration and the like. According to the method, only vanadium is extracted through synergistic extraction, impurity cations are not extracted, the vanadium purity of a loaded organic phase is improved, hydrogen peroxide is catalytically decomposed (the catalyst can be recycled), vanadium is reversely extracted through sulfuric acid, the procedures of reduction or oxidation and the like are omitted, material consumption and energy consumption are reduced, introduction of impurities is reduced, the purity of the obtained vanadium electrolyte is larger than 99.9%, and the impurity concentration is lower than 10 mg / L; compared with an existing vanadium-containing liquid vanadium purification and enrichment technology, the total vanadium recovery rate is increased by 10%-20%, the process is simplified by 50%, the production cost is reduced, the environment is protected, and the method has high application and popularization value.

The invention provides a preparation method of Myc tag antibody magnetic beads, and belongs to biomedicine. The preparation method comprises the following steps: washing magnetic beads, adding the washed magnetic beads into a magnetic bead activation buffer solution, activating for 20-60 minutes, coupling the activated magnetic beads with an ultra-filtered Myc tag antibody, adding the magnetically separated magnetic beads into a confining liquid, sealing at 4 DEG C for 2 hours, cleaning, and adding a preserving fluid. And the prepared Myc tag antibody magnetic bead also has relatively good specificity, enrichment capacity and stability.

The invention belongs to the technical field of hydrogel, and mainly relates to preparation and application of hydrogel with a doughnut structure. The invention aims to solve the problem that the existing seawater desalination material based on hydrogel is insufficient in recyclability. The preparation method comprises the following steps: mixing ZrOCl2. 8H2O and fumaric acid, and performing ultrasonic dispersion on the mixture and a multi-walled carbon nanotube to obtain a pretreated precursor; carrying out hydrothermal treatment on the pretreated precursor, and modifying the pretreated precursor with acryloyl chloride to obtain modified MOF801 (at) CNT; the modified MOF801-coated CNT, polyvinyl alcohol, cassava starch and a cross-linking agent are subjected to thermosensitive cross-linking treatment, and the hydrogel with the doughnut structure is obtained. The hydrogel has high application value in the field of seawater desalination, has self-adjustability of temperature response, is high in evaporation efficiency, can be recycled, and can efficiently desalinate seawater with different temperatures and components.

The invention relates to a passive sampler and a detection method for organophosphorus esters and converted products thereof, which are characterized in that on the basis of an organic silica gel wrist strap, the novel passive sampler is designed by combining the organic silica gel wrist strap with a solid-phase extraction filler technology, and an SPE filler is used as a sampling medium of OPEs in air, so that the defects of a single silica gel wrist strap are overcome; the OPEs monitoring can be better served; the passive sampler comprises a nylon glass fiber shell, a polyethylene sieve plate and a full fluororubber gasket are arranged in the nylon glass fiber shell, HC-C18 / CN composite filler is contained in a formed space, and a silica gel ring can be arranged on the outer side of the nylon glass fiber shell in a sleeving mode. The composite passive sampler disclosed by the invention has the beneficial effects that compared with a single silica gel material, the composite passive sampler prepared by the invention has the advantages that the adsorption curves of OPEs and di-OPEs are improved; particularly, the effect of remarkably increasing the adsorption rate and the adsorption capacity on aromatic OPEs such as TPHP and TCRP is achieved.

The trace substance enrichment filter sequentially comprises a filter support, a flow guide assembly, a plurality of sets of filter assemblies and a bottom plate which are coaxially arranged from top to bottom, the filter support comprises a top plate, a plurality of supporting rings are fixedly connected to the periphery of the bottom of the top plate in a surrounding mode, the flow guide assembly comprises an annular flow guide frame, and the flow guide frame is fixedly connected with the bottom of the top plate in a surrounding mode. A flow guide plate is installed in the flow guide frame, each group of filtering assemblies comprises an annular filtering frame, a filtering supporting plate is installed in the filtering frame, a filtering membrane is laid on the top of the filtering supporting plate, a water outlet is formed in the bottom plate, and a plurality of screw rods extending vertically and upwards are arranged on the periphery of the top of the bottom plate; and each screw rod sequentially penetrates through the plurality of filtering frames, the flow guide frame, the supporting ring and the top plate and is screwed and fixed with a nut. According to the device, trace substances can be directly filtered and enriched within 6000 meters underwater, the process of collecting a large amount of seawater to a laboratory is omitted, and the device is convenient to operate and high in working efficiency.

This invention provides an absorbent for capturing carbon dioxide and a method for capturing carbon dioxide. By volume percentage, the absorbent comprises 20-60% glycine salt aqueous solution, 10-50% triethylene glycol monomethyl ether, 5-10% ethylene glycol, and the balance being water. This absorbent combines the advantages of glycine salt systems, such as low volatility, non-toxicity, safety, and better antioxidant degradation resistance, while avoiding the risk of solid precipitates forming after absorption by the glycine salt system, which could cause pipeline blockage.

The invention relates to a method for recovering nickel through alkaline sodium salt roasting of an FCC waste catalyst, and relates to the technical field of waste catalyst recycling, the method for recovering nickel through alkaline sodium salt roasting of the FCC waste catalyst comprises the following steps: stirring and mixing an FCC waste catalyst raw material and an alkaline sodium salt agent to obtain sodium salt roasting mixed powder; the sodium salt roasting mixed powder is roasted, and roasted powder is obtained; and adding a sodium hydroxide solution into the roasted powder for heating and leaching treatment, and then filtering and washing to obtain nickel oxide. According to the method for recovering nickel through alkaline sodium salt roasting of the FCC waste catalyst, the ideal nickel enrichment effect can be achieved at the temperature of 900 DEG C in the roasting process, compared with a reduction smelting method, the process temperature is low in energy consumption, and the problems that a traditional high-temperature reduction roasting method is large in energy consumption and high in cost are solved.

The invention relates to a method for detecting trace nano plastic in biological fluid by combining magnetic solid-phase extraction and surface enhanced Raman spectroscopy. The method for detecting the trace nano plastic in the biological fluid by combining the magnetic solid-phase extraction and the surface enhanced Raman spectroscopy comprises the following steps: enriching the nano plastic in a biological fluid sample by using aminated magnetic beads to obtain an aggregate, and desorbing the aggregate to obtain a desorption solution, and mixing the desorption solution with an SERS enhanced substrate solution, drying and detecting. According to the method, the strong enrichment capacity of the aminated magnetic beads and the excellent performance of the SERS enhanced substrate are combined, qualitative and quantitative analysis of trace multi-size nano plastic in biological fluid is achieved, and the method has the advantages of being easy to operate, high in reproducibility and the like. The detection limit of polystyrene nano plastic in urine and serum is as low as 6 * 10 <-7 > g / L, and the quantitative analysis capability is strong (R2gt; and the standard addition recovery rate in urine and serum is excellent.

The invention discloses a preparation method of an ultra-high-sensitivity gold star nanoparticle / MOF-808 SERS (Surface Enhanced Raman Scattering) substrate, which comprises the following steps: firstly, synthesizing sea urchin-shaped gold star nanoparticles (GNS) with a multi-tip structure in the presence of an amphiphilic block copolymer by a seed mediated growth method; then, by taking zirconium oxychloride octahydrate and 1, 3, 5-benzene tricarboxylic acid as precursors, performing hydrothermal reaction to synthesize an MOF-808 metal organic framework carrier; finally, GNS and MOF-808 are combined in a self-assembly mode, and the solid SERS substrate is obtained through ultrasonic loading, centrifugation, freeze drying and grinding. The method is simple and convenient in preparation process and low in cost, and the prepared substrate is high in uniformity, good in signal reproducibility and high in sensitivity and shows excellent structural stability. Wherein the multi-tip structure of the sea urchin-shaped aventuris effectively enhances a local electromagnetic field, and the SERS detection sensitivity is remarkably improved.

This invention provides a front-end treatment device for electroplating wastewater contaminated with heavy metals, relating to the field of electroplating wastewater technology. The device includes a first treatment chamber, a transmission chamber, a mounting rod, a permanent magnet, a connecting rod, a second treatment chamber, a connecting pipe, and a stirring assembly. This invention uses neodymium iron boron (NdFeB) permanent magnets as the core element for heavy metal adsorption. These magnets possess extremely strong magnetic adsorption properties, rapidly adsorbing magnetic heavy metal ions such as iron, nickel, and cobalt, as well as pollutants containing magnetic components, from electroplating wastewater. Simultaneously, the permanent magnets are driven by a swing assembly to oscillate reciprocally at an angle of 30–60°. Combined with the opposite polarity of adjacent permanent magnets, a dynamically changing magnetic field environment is formed. This not only expands the magnetic field coverage but also avoids the formation of adsorption saturation regions, significantly improving the capture efficiency of heavy metal ions. The titanium alloy or polyvinyl chloride corrosion-resistant protective shell on the outer surface of the permanent magnets ensures magnetic stability and extends service life, guaranteeing long-term and efficient heavy metal separation.

The application relates to the technical field of new materials, in particular to a preparation method and application of a covalent organic framework nanometer noble metal composite material, which comprises the following steps: monomer dissolving, mixing, centrifugal precipitation, washing and drying, gold-silver ion reaction and composite material dispersion. The preparation method and application of the covalent organic framework nanometer noble metal composite material, the COF-Au@Ag composite material has good adsorption performance and enrichment capacity for new pollutants such as antibiotics and microplastics, and can be used as a surface enhanced Raman substrate to efficiently enrich and sensitively detect trace antibiotics and microplastics in complex plant samples.

The invention provides a magnetic solid-phase extraction method for indole compounds in Baijiu and application of the magnetic solid-phase extraction method, and belongs to the technical field of food detection. The magnetic solid-phase extraction method comprises the following steps: adsorbing indole substances (IAA and IPA) in Baijiu by using a GO-PAA-Fe3O4 composite material as an adsorbent, and then desorbing in an acidic acetone desorption solvent to complete the magnetic solid-phase extraction of the indole compounds. The method provided by the invention can effectively overcome the defects of low concentration of indole substances in Baijiu, complex matrix interference, poor enrichment effect, complex detection, consumption of a large amount of organic reagents and the like in the prior art.

The invention belongs to a fertilizer mixture containing an insecticide, and particularly relates to a dinotefuran-containing compound fertilizer and a preparation method thereof. The dinotefuran-containing compound fertilizer is composed of the dinotefuran slow-release agent and the water-soluble matrix, the dinotefuran slow-release agent has a good slow-release characteristic at different temperatures and different pH values, dinotefuran has two behaviors of diffusion and corrosion under the corresponding pH value condition, and the water-soluble matrix is wide in material source and low in cost. Agricultural and animal husbandry wastes are fully utilized, selection can be performed in combination with an actual application scene of a compound fertilizer, and the water-soluble matrix obtained by combining earthworm composting with acid treatment contains rich nutrient substances and can meet plant growth and development. The obtained water-soluble matrix has a good stabilizing effect on the dinotefuran slow-release agent and can be replaced by other water media, and the water-soluble matrix and the dinotefuran slow-release agent have a good matching effect. The compound fertilizer obtained by the invention has a relatively good insecticidal effect and a relatively good nutrient substance supplementing effect.

The invention relates to the technical field of biology, in particular to a detection method of gastrin 17. According to the invention, the gastrin 17 in serum / plasma is quantified by an immune enrichment method. According to the pretreatment method, the gastrin 17 in the serum is enriched by adopting the magnetic beads coated with the gastrin 17 specific antibody, the enrichment rate close to 100% can be obtained by uniformly mixing and incubating for 20 minutes at a low speed, the magnetic bead immune enrichment process is optimized in detail, the pretreatment operation is simple and convenient, the enrichment effect is good, and the detection sensitivity of the method is high (2pg / ml). Liquid phase conditions are optimized and determined, and a detection method with excellent performance is developed; according to the method, the magnetic bead enrichment efficiency and the absolute recovery rate are carried out, linearity, sensitivity and standard recovery performance index confirmation is carried out, and the performance is excellent.

The invention discloses a phage display library panning method based on elastic tag-like recombinant protein, and belongs to the technical field of biology. According to the method, the reversible phase change characteristic of elastin-like proteins (ELPs) is utilized, a target antigen sequence used for elutriation is fused with the ELPs, an antibody of an anti-specific antigen sequence displayed by phage is elutriated from a solution through multiple reversible phase change cycles, that is, the antibody displayed by the phage and combined with the specific antigen can be obtained through enrichment through multiple rounds of elutriation and amplification, and the specific antigen sequence of the ELPs is detected. The process only needs a conventional centrifugal machine and a constant-temperature water bath kettle, has the advantages of low cost, simplicity and convenience in operation and the like, and provides a new idea for elutriation of the phage display library.

The invention provides a probe, a primer, a kit, a method and application for human adenovirus whole genome sequencing. Aiming at a conserved sequence in an adenovirus genome, a plurality of pairs of primer probes are explored. According to the primer pair and the probe, the coverage degree of different adenovirus type genomes is greatly improved, and meanwhile, the amplification capacity of the primer aiming at targets with different lengths is enhanced. Eight adenovirus whole-genome sequences can be uniformly amplified, so that not only is the coverage degree of the whole-genome sequences improved, but also the capturing capacity for targets with different lengths is enhanced. In addition, the method has high tolerance for samples with different qualities, samples with low concentration or samples degraded to a certain degree can be subjected to high-uniformity total-sequence capture, and the method has high sensitivity and uniformity.

The invention relates to the technical field of detection of materials for cigarettes, in particular to a method for detecting Pb < 2 + > in glyceryl triacetate for cigarettes. The method comprises the following steps: 1, adding an actual sample into a centrifugal tube, and then adding a nitric acid solution with the mass fraction of 5%; 2, an APDC solution with the concentration of 0.01 M is added, the ratio of the concentration of APDC to the concentration of Pb < 2 + > in an actual sample solution is made to be larger than 100: 1 all the time, and it is ensured that Pb < 2 + > is fully complexed; step 3, adding N, N-dimethyl benzylamine; 4, sodium hydroxide with the concentration being 200 g / L is added; 5, centrifuging, transferring 200 microliters of an upper-layer N-N dimethyl benzylamine phase into a centrifugal tube, and adding a nitric acid solution with the mass fraction of 5% for dilution; step 6, detecting Pb < 2 + > in the diluted solution by adopting square wave anodic stripping voltammetry; the detection method has the advantages of simple pretreatment process, accurate detection result and low cost.

The invention discloses a method for determining 13 metabolic regulation core molecules under a same chromatographic system and the construction of a detection discrimination model thereof; 13 metabolic regulation core molecules are methionine, homocysteine, cysteine, vitamin B2, 5-methyltetrahydrofolate, folic acid, pyridoxal phosphate, 4-pyridoxic acid, methylmalonic acid, betaine, choline, S-adenosylmethionine, S-adenosylhomocysteine. Low concentration acetic acid is used as a mobile phase additive in the detection system of the present invention, so that choline with very high polarity can be well retained on the chromatographic column, the response is also greatly increased, and the S-adenosylmethionine matrix effect is greatly reduced by WCX enrichment, and trace S-adenosylmethionine in serum can be accurately determined, further combined with specific chromatographic column selection, etc., it is achieved that 13 compounds are accurately determined under the same chromatographic system, and a discrimination model is established simultaneously, and a rapid interpretation of the clinical determination results of the 13 compounds is achieved.

The invention relates to the technical field of new materials, in particular to a magnetic solid-phase extractant based on carbon-coated Fe3O4 and a preparation method and application thereof.The solid-phase extractant comprises a Fe3O4 core, a carbon layer and a covalent organic framework, the carbon layer coats the surface layer of the Fe3O4 core, and the covalent organic framework is fixed to the carbon layer; the surface of the carbon layer is modified with hydroxyl and amino chemical bonding sites, the carbon layer covering the surface can effectively protect the magnetic inner core from being oxidized, the stability of the magnetic material structure is kept, and the agglomeration phenomenon caused by long-time exposure is avoided; the solid-phase extraction agent has good adsorption and enrichment effects on estrogen compounds, and is expected to be used for enrichment and determination of the estrogen compounds in samples such as food and plasma through liquid-phase system analysis; the preparation method of the solid-phase extraction agent is simple and convenient, easy to operate and beneficial to large-scale production.

The invention belongs to the technical field of surface enhanced Raman scattering (SERS) substrates, and provides a magnetic ternary composite SERS substrate capable of being degraded and recycled through photocatalysis as well as a preparation method and application of the SERS substrate. The method comprises the following steps: carrying out modification treatment on Co / C or Co / C / g-C3N4 by using (3-aminopropyl) triethoxysilane, so as to obtain modified Co / C or modified Co / C / g-C3N4; mixing the modified Co / C with the Ag NPs solution to obtain Co / C / Ag; co / C / Ag and g-C3N4 are mixed for incubation, Co / C / Ag / g-C3N4 is obtained, or modified Co / C / g-C3N4 and an Ag NPs solution are mixed, and Co / C / g-C3N4 / Ag is obtained. The magnetic ternary composite SERS substrate capable of being degraded and recycled through photocatalysis is designed and prepared, and very excellent enhanced Raman performance and visible light catalysis effect are achieved.

This invention discloses a covalent organic framework composite membrane, its preparation method, and its applications, belonging to the fields of materials chemistry and analytical chemistry. The covalent organic framework composite membrane comprises a porous polymer substrate membrane, a polydopamine-mediated layer, and a covalently bonded functional layer. The functional layer is formed by linking 2,5-dihydroxy-1,4-phenylenedialdehyde and 5',5''-bis(4-aminophenyl)-[1,1':3',1'':3'',1'''-tetraphenyl]-4,4'''-diamine via imine bonds. This invention achieves controllable and robust growth of a novel COF on the surface of an inert polymer membrane through a "polydopamine-mediated layer + aldehyde pre-anchoring" strategy. As a solid-phase extraction medium, the composite membrane exhibits excellent enrichment performance and good reusability for trace amounts of carbamate pesticides in food and environmental samples, and has significant application value in sample pretreatment.

The invention provides a gene combination and a polygene expression cassette for improving cadmium enrichment and cadmium resistance of plants, and relates to the technical field of biological gene engineering. A PPKS gene, an MT1ES gene, an MT2S gene, an MT3S gene and an MT4S gene are combined, gene expression cassettes are constructed respectively after codon optimization is performed according to codon preference of a target plant, and the five gene expression cassettes are connected in series into a plant transformation vector to obtain a recombinant plant transformation vector. And transforming the recombinant plant transformation vector into a target plant to obtain an engineering plant capable of efficiently expressing an exogenous gene in the target plant. Experimental determination results prove that transfer of the PPKS gene, the MT1ES gene, the MT2S gene, the MT3S gene and the MT4S gene improves the enrichment capacity and tolerance of the plant to cadmium, so that the engineering plant has a more ideal cadmium-contaminated soil remediation effect.

This invention proposes a method for recovering potato protein based on dielectrophoretic microfluidic technology, belonging to the field of plant protein separation and recovery technology. This invention addresses the problems of existing dielectrophoretic microfluidic technologies, which are mostly designed for pure protein systems, have poor adaptability to complex wastewater, and have limited enrichment capacity for low-concentration, small-molecule proteins. The steps of this invention include: S1: frequency response and force analysis of potato protein; S2: separation chip simulation analysis; S3: introducing starch wastewater into a microchannel and applying an AC voltage to the electrode structure according to the AC electric field parameters, causing the potato protein to migrate directionally under the action of dielectrophoretic force in a non-uniform electric field, thus separating it from other particles; S4: collecting the directionally migrated potato protein to achieve recovery, and evaluating the recovery effect of the potato protein.

This application belongs to the field of hydrometallurgical technology, specifically relating to an efficient method for recovering molybdenum during the sulfuric acid-enhanced decomposition of tungsten ore. The method includes the following steps: S1, obtaining tungsten ore, mixing it with concentrated sulfuric acid, and roasting it to obtain a roasting product. The roasting product, by mass percentage, includes: WO3: 10%-60%, Mo: 0.01%-5%; S2, mixing the roasting product with acidic washing liquid to obtain a first mixture. Under stirring conditions, a selective complexing agent is added to the first mixture to induce a complexation reaction, resulting in a second mixture. The second mixture is then subjected to solid-liquid separation to obtain a solid slag and a complexing liquid. The selective complexing agent includes at least one of thiourea and its derivatives or sulfur-containing organic compounds; S3, recovering molybdenum from the complexing liquid. This application, by introducing selective complexation during the washing stage, enriches the complexing liquid with over 98.5% of the molybdenum, while the residual rate of tungsten in the complexing liquid is ≤0.01%, thus solving the problem of molybdenum dispersion and loss in the slag phase and washing water.